CN108931648A - A kind of cystatin C detection kit based on latex immunoturbidimetry - Google Patents
A kind of cystatin C detection kit based on latex immunoturbidimetry Download PDFInfo
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Abstract
The invention discloses a kind of cystatin C detection kits based on latex immunoturbidimetry, it includes reagent R1 and reagent R2, reagent R1: buffer, inorganic salts, preservative, increasing milk agent, stabilizer, reagent R2: buffer, increasing milk agent, surfactant, stabilizer, preservative, the latex particle for being coated with cystatin C antibody sensitized；The latex particle of the sensitization is the polystyrene latex microspheres after quadratic convergence polymerization, reduces range of linearity high level sensitivity；The increasing milk agent balances influence of the quadratic convergence polymerization to low value sensitivity, improve antigen excess detection range, this kit effect ground improves measured object concentration caused by HOOK effect and is underestimated or even occurred false negative result, and the performances such as its repeatability and stability are unaffected, it is easy to operate, there is extensive versatility.
Description
Technical field
The invention belongs to technical field of immunoassay, and in particular to a kind of cystatin C inspection based on latex immunoturbidimetry
Test agent box.
Background technique
Cystatin C (CystatinC, abbreviation Cys C) is also known as cysteine proteinase inhibitor C, is one kind by 120 ammonia
Base acid composition, low molecular weight, alkaline non-glycated protein of the molecular weight for 13kDa.The gene of coding Cys C belongs to house keeper's base
Cause, can be in all karyocytes with constant speed processive transcription and expression, inorganization specificity.Cys C is present in various
Among body fluid, it is especially height with content in cerebrospinal fluid and refining, also may be present in blood, in saliva, it is minimum in urine, not by year
The factors such as age, gender, weight, inflammation influence, research shows that Cys C is from after 1 years old to constant concentration in blood before 60 years old.Cause
Cys C molecular weight is small, positively charged under physiological condition, can be inhaled again by renal cells completely freely from glomerular filtration
Receive and in intracellular degradation, do not come back in blood；Simultaneously renal cells also do not secrete to lumen, make kidney at
For the unique place for removing Cys C in circulation.
Clinical evaluation kidney trouble progress and severity are generally reference with renal function, and renal function is generally with glomerulus
Filtration rate (GFR) reflection.The endogenous marker of common evaluation detection of glomeruli filtration function has serum creatinine (Scr), urea at present
Nitrogen (Urea), endogenous creatinine clearance rate (Ccr) etc..But these indexs will receive the age, gender, height, gender, muscle mass,
The influence of diet structure, organism disease situation, drug and renal tubule to factors such as the secretions of creatinine, is not able to satisfy ideal endogenous
The requirement of marker.Kidney is the unique place for removing Cys C in circulation, and renal tubule does not secrete Cys C, thus change of serum C ys C
Concentration mainly determines by GFR, and not by the heart not, the age, inflammatory reaction, tumour, muscle activity, the factors such as diet intake shadow
It rings.Cys C high sensitivity is good with GFR correlation.Many clinical observations confirm that, when renal function is slightly damaged, Scr variation is unknown
When aobvious, change of serum C ys C has increased, either slightly, moderate, severe renal function reduce, corresponding change of serum C ys C value is all shown
Good sensitivity.Cys C is the endogenous material for substantially meeting ideal endogenous GFR marker requirement so far, is newest hair
The index that a kind of sensibility for the assessment renal function that exhibition is got up is good, specificity is high.
Latex immunoturbidimetry is relatively stable, the accurate immunoturbidimetry method of one kind occurred in recent years.Basic principle
It is that test substance corresponding antibodies are linked to latex particle surface in a specific way, when the latex particle and antigen for being crosslinked with antibody
In conjunction with rear, aggregation certain turbidity can be generated rapidly, change the light transmission of reaction solution.The change of reaction solution light transmission and anti-
Original content has correlation, can reflect the antigen concentration in test substance.The method can be applied to automatic clinical chemistry analyzer,
And the used time is short, is suitable for clinical detection.
But presently commercially available detection kit is in process of clinical application, one timing of antibody concentration in reaction system, with
The increase of antigen concentration, antigen antibody complex precipitation capacity increase, progressively reach balance, when antigen concentration is excessively high, will occur
Depolymerization even non-setting situation, i.e. HOOK effect or antigen excess phenomenon are precipitated, in immune detecting system, which will be led
It causes measured object concentration to be underestimated or even occurred false negative result appearance, may cause mistaken diagnosis or fail to pinpoint a disease in diagnosis.Those skilled in the art's root
According to the experience of a large amount of experimental study, antibody and antigen ratio etc. are adjusted using reagent sensitivity, antibody dosage, dilution method is improved
Method, there is HOOK effect in delay in detection reagent to a certain extent, i.e. expansion antigen excess detection range.But it is simple
The reagent detection range of linearity is reduced while improving reagent sensitivity；Adjust antibody dosage or dilution method adjustment antibody and antigen
The R&D cycle that ratio needs is long, R&D work amount is big and cost is excessively high.
CN107894509A discloses a kind of method for improving latex immunoturbidimetry antigen excess and the range of linearity, mainly
It is by the way of multiple cross-flow filtration, using lithium chloride as regulator, neopelex (SDBS) is emulsifier, over cure
Change potassium is initiator, has directly carried out quadratic convergence polymerization to latex particle and has improved.But this method process needs to recycle cross-flow
Filtering, it is complicated for operation.The present invention is to optimize on the basis of improving to this method to kit of the present invention, is expanded
Reagent linearity test and antigen excess range improve the accuracy to high concentration pattern detection, effectively improve HOOK effect and lead
False negative result is underestimated or even occurred to the measured object concentration of cause.
Summary of the invention
To solve the above-mentioned problems, the cystatin C detection reagent based on latex immunoturbidimetry that the present invention provides a kind of
Box, the kit forms are simple, have extensive versatility, can effectively improve HOOK effect, improve the accuracy of testing result.
Realize above-mentioned purpose, the invention adopts a technical scheme as:
A kind of cystatin C detection kit based on latex immunoturbidimetry, including reagent R1 and reagent R2, the reagent R1 packet
Include inorganic salts 0.1-120g/L, buffer 20-200 mmol/L, increasing milk agent 0.8mmol/L-4.2 mmol/L, preservative 0.8-
3.2g/L, stabilizer 1-200g/L；The reagent 2 includes buffer 20-200 mmol/L, increasing milk agent 0.8mmol/L-4.2
Mmol/L, surfactant 1-20g/L, stabilizer 1-200g/L, the latex particle 0.1- for being coated with cystatin C antibody sensitized
3g/L, preservative 0.5-5g/L, surplus are water, and wherein water is purified water.
It is 0.1-1.5g/L magnesium chloride and calcium chloride or 15-120g/L chlorine that inorganic salts, which are by melting concn, in the reagent R1
Change sodium, the latex microsphere that selected magnesium chloride can effectively inhibit quadratic convergence to polymerize with the mixture of calcium chloride or sodium chloride
It is swollen, enhances the stability of kit high level sensitivity passivation.
The buffer is one of trihydroxy methyl amino buffer, phosphate buffer or glycine buffer.
The stabilizer is that one of trehalose, sucrose, glycine and brij-35 or multiple combinations form.The increasing
Emulsion is PEG2000~PEG20000.
The buffer is trihydroxy methyl amino buffer；The preservative is PC300；The stabilizer is trehalose.
The increasing milk agent is PEG6000, and the main function of selected PEG6000 is the sensitivity for improving kit, balance
Low value sensitivity decrease caused by quadratic convergence polymerize.
The surfactant is one of polysorbas20, polysorbate60, Tween 80 and Triton X-100.
The preservative is one of Sodium azide or PC300.
Coated on the latex particle of the sensitization is that the anti-human cystatin C of animal is polyclonal or monoclonal antibody.
The latex particle of the sensitization is the polystyrene latex microspheres after quadratic convergence polymerization, reduces range of linearity height
It is worth sensitivity, and low value sensitivity is unaffected, improves antigen excess detection range, the repeatability not influenced and stability etc.
Performance.
The latex microsphere preparation method of quadratic convergence polymerization the following steps are included:
1) use the method for high speed refrigerated centrifuge that latex microsphere solution replacement for distilled water, is placed in reaction kettle；
2) mixed emulsifier and regulator is added, after being passed through N2 displacement O2, initiator is added, causes after polymerization；
3) buffer that emulsion reagent label reaction needs is added to be centrifuged.
The refrigerated centrifuge is under the conditions of 4 DEG C, is that 20000r/min is centrifuged with revolving speed, the time is 15 minutes.
The mixed emulsifier be in mass ratio be 1:1SDS and NP-40；The regulator is lauryl mercaptan；Institute
Stating initiator is over cure potassium.
0.72 times for completing partial size when the latex microsphere partial size of convergence polymerization reaction is unpolymerized.
The invention has the following advantages:
The present invention makes this under the premise of not influencing with antibody-binding energy using the method for latex microsphere quadratic convergence polymerization
Kit expands antigen excess detection range, Lower result caused by reducing because of HOOK effect to the sensitivity decrease of high level
Or a possibility that false negative.It is balanced using increasing milk agent because quadratic convergence polymerize bring low value sensitivity decrease, makes the reagent
The low value sensitivity of box is unaffected.Kit of the present invention can be effectively prevented generation using certain density inorganic ion
The latex microsphere of convergence polymerization is swollen in the solution, stablizes its high level sensitivity decrease property retention.Examination of the invention
Agent box can effectively expand antigen excess detection range, and the polymerization of easy to operate and latex microsphere quadratic convergence will not influence its essence
The performances such as density, stability.
Specific embodiment
Below with reference to example, invention is further described in detail, it should be understood that the scope of protection of the invention not only limits
In the range of these examples.
The preparation of embodiment 1 does not contain the kit of the present invention of increasing milk agent
Kit of the present invention includes reagent R1 and reagent R2 biliquid component independent of each other, wherein
Reagent R1:
Trihydroxy methyl amino buffer 100mmol/L
Magnesium chloride 0.5g/L
Calcium chloride 0.5g/L
PC300 1.2g/L
Trehalose 5g/L
Surplus is purified water
Reagent R2:
Trihydroxy methyl amino buffer 100mmol/L
Glycine 5g/L
Polysorbas20 5g/L
PC300 1.2g/L
Bovine serum albumin(BSA) 5g/L
Anti-human cystatin C polyclonal antibody sensitizing latex particle 5g/L
Surplus is purified water.
The used specific preparation process of latex microsphere:
Firstly, the displacement of latex microsphere solution.The latex microsphere solution for being 10% by 200ml mass concentration
In refrigerated centrifuge, wherein the partial size of latex microsphere is 141nm, is centrifuged with the condition of 4 DEG C, 20000r/min,
Time is 15 minutes.Latex microsphere solution after centrifugation is discarded, distilled water is added, is uniformly mixed；
Secondly, the latex microsphere solution after displacement distilled water is added in the reaction kettle that specification is 1L, the quality of 0.6g is added
Mixed emulsifier and 0.16g lauryl mercaptan than SDS and NP-40 for 1:1, are adjusted to 50- for the temperature of reaction kettle
60 DEG C, after temperature is constant, setting revolving speed is that 300r/min stirs 20min.Then pass to the oxygen 3min in nitrogen removal solution
Afterwards, 0.08g potassium peroxydisulfate is added, causes the quadratic convergence polymerization reaction of latex microsphere, continues to stir with identical temperature and revolving speed
Mix 12h；
Finally, by complete quadratic convergence polymerization reaction after latex microsphere solution with the condition of 4 DEG C, 20000r/min carry out from
The heart, time are 15 minutes.Latex microsphere solution after centrifugation is discarded, the Tris solution of 50mM/L concentration, pH5.0 is added, is mixed
It closes uniform.
The partial size of latex microsphere is 141nm in latex solution before quadratic convergence polymerization reaction, and the partial size of latex is after reaction
D2=0.72D=102nm。
Embodiment 2 prepares the kit of the present invention containing increasing milk agent
Kit of the present invention includes reagent R1 and reagent R2 biliquid component independent of each other, wherein
Reagent R1:
Trihydroxy methyl amino buffer 200mmol/L
Magnesium chloride 0.3g/L
Calcium chloride 0.3g/L
PC300 2.0g/L
Trehalose 5g/L
PEG6000 2.0mmol/L
Surplus is purified water
Reagent R2:
Trihydroxy methyl amino buffer 200mmol/L
Glycine 5g/L
Polysorbas20 5g/L
PC300 2.0g/L
Bovine serum albumin(BSA) 5g/L
PEG6000 2.0mmol/L
Anti-human cystatin C polyclonal antibody sensitizing latex particle 5g/L
Surplus is purified water.
The used specific preparation process of latex microsphere:
Firstly, the displacement of latex microsphere solution.The latex microsphere solution for being 10% by 200ml mass concentration
In refrigerated centrifuge, wherein the partial size of latex microsphere is 141nm, is centrifuged with the condition of 4 DEG C, 20000r/min,
Time is 15 minutes.Latex microsphere solution after centrifugation is discarded, distilled water is added, is uniformly mixed；
Secondly, the latex microsphere solution after displacement distilled water is added in the reaction kettle that specification is 1L, the quality of 0.6g is added
Mixed emulsifier and 0.16g lauryl mercaptan than SDS and NP-40 for 1:1, are adjusted to 50- for the temperature of reaction kettle
60 DEG C, after temperature is constant, setting revolving speed is that 300r/min stirs 20min.Then pass to the oxygen 3min in nitrogen removal solution
Afterwards, 0.08g potassium peroxydisulfate is added, causes the quadratic convergence polymerization reaction of latex microsphere, continues to stir with identical temperature and revolving speed
Mix 12h；
Finally, by complete quadratic convergence polymerization reaction after latex microsphere solution with the condition of 4 DEG C, 20000r/min carry out from
The heart, time are 15 minutes.Latex microsphere solution after centrifugation is discarded, the Tris solution of 50mM/L concentration, pH5.0 is added, is mixed
It closes uniform.
The partial size of latex microsphere is 141nm in latex solution before quadratic convergence polymerization reaction, and the partial size of latex is after reaction
102nm。
Embodiment 3 is not added with the linear low value of PEG6000 reagent and high level sensitivity technique
Using 1 reagent preparation of above-described embodiment, the sensitivity of linear low value and high level concentration samples is detected, and with it is current
Bladder chalone C determining reagent kit (the latex immunoturbidimetry for being D=100nm without quadratic convergence polymerization partial size to circulate in the market
Method) it is compared (purchased from hundred Ao Taikang Bioisystech Co., Ltd of Beijing) as control.Compound concentration be S=0.45,0.90,
1.80,3.75,7.50mg/L cystatin C sample solution repeats each concentration samples solution using embodiment 1 and contrast agent
Detection 3 times, testing result is shown in Table 1.
Table 1
As can be seen from Table 1, the kit of the present invention that latex microsphere is prepared after being polymerize using quadratic convergence is being not added with PEG6000
When, low value concentration measurement (S=0.45) lower than contrast agent measured value 10%.When high level concentration value (S=7.50) measures, compare
Lower than reagent measured value 33%.When illustrating that reagent does not add increasing milk agent, low value sensitivity and high level sensitivity are in passivation shape
State.
Embodiment 4 adds the linear low value of PEG6000 reagent and high level sensitivity technique
Using 2 reagent preparation of above-described embodiment, the sensitivity of linear low value and high level concentration samples is detected, and with it is current
Bladder chalone C determining reagent kit (the latex immunoturbidimetry for being D=100nm without quadratic convergence polymerization partial size to circulate in the market
Method) it is compared (purchased from hundred Ao Taikang Bioisystech Co., Ltd of Beijing) as control.Compound concentration be S=0.45,0.90,
1.80,3.75,7.50mg/L cystatin C sample solution repeats each concentration samples solution using embodiment 1 and contrast agent
Detection 3 times, testing result is shown in Table 2.
Table 2
As can be seen from Table 2, the kit of the present invention that latex microsphere is prepared after being polymerize using quadratic convergence is in addition PEG6000
When, low value concentration measurement (S=0.45) is not much different with contrast agent measured value.When high level concentration value (S=7.50) measures, though
So increased, but it is still lower by 20% or more than contrast agent measured value.Illustrate after adding increasing milk agent PEG6000, low value is sensitive
Degree balances the influence of quadratic convergence polymerization, and high level sensitivity is still within passive state.
The detection of 5 antigen excess sample of embodiment
Using 2 reagent preparation of above-described embodiment, antigen excess sample is detected, and with circulate currently on the market without
It is (difficult to understand safe purchased from Beijing hundred to cross the bladder chalone C determining reagent kit (latex immunoturbidimetry) that quadratic convergence polymerization partial size is D=100nm
Health Bioisystech Co., Ltd) it is compared as control.Compound concentration be S=7.50,15.00,30.00,60.00,
100.00,200.00,400.00,800.00,1000.00,2000.0mg/L cystatin C sample solution uses 2 He of embodiment
Contrast agent repeats detection 3 times to each concentration samples solution, and testing result is shown in Table 3.
Table 3
Table 3 is the results show that reagent of the present invention, and on the basis of the range of linearity does not change, antigen excess detection range is obtained
Great raising.When concentration of specimens reaches 2000.00mg/L, detected value still can exceed that the range of linearity, and contrast agent is in sample
Detected value is in the range of linearity when this concentration is greater than 200.00mg/L, and with the increase of concentration of specimens, detected value is gradually
Reduce, even in reference range.Therefore, cystatin C detection kit of the present invention can satisfy antigen excess range and reach
To the requirement of 2000.00mg/L.
6 repeatability detection of embodiment
Using 2 reagent preparation of above-described embodiment, the repeatability of kit of the present invention is studied, and with circulate currently on the market
Bladder chalone C determining reagent kit (latex immunoturbidimetry) (be purchased from hundred Ao Taikang Bioisystech Co., Ltd of Beijing) as control
It compares.Calibration object and quality-control product are Landau 1206CY and 1202CY respectively, the use of instrument are automatic clinical chemistry analyzer
TBA-40FR, parameter setting is as follows, wavelength 546nm, and the Direction of Reaction is positive direction, sample size: reagent 1: reagent 2(embodiment 2
Reagent or control)=3 μ L:200 μ L:50 μ L.Detection 10 times is respectively repeated to embodiment 2 and contrast agent, calculates separately the equal of measured value
The ratio of value and standard deviation (SD), mean value and standard deviation is variation within batch coefficient (CV), the results are shown in Table 4.
Table 4
Table 4 the result shows that, the variation within batch coefficient CV of reagent of the present invention is respectively less than 10%, the variation within batch coefficient with contrast agent
CV is close, and the variation within batch coefficient for meeting general diagnostic reagent is not more than 10% requirement.Thus illustrate the repetition of reagent of the present invention
Property it is good, the polymerization of the quadratic convergence of latex microsphere will not influence reagent repeatability.
7 Detection of Stability of embodiment
Using 2 reagent preparation of above-described embodiment, the stability of kit of the present invention is studied, and with circulate currently on the market
Bladder chalone C determining reagent kit (latex immunoturbidimetry) (be purchased from hundred Ao Taikang Bioisystech Co., Ltd of Beijing) as control
It compares.Calibration object and quality-control product are Landau 1206CY and 1202CY respectively, the use of instrument are automatic clinical chemistry analyzer
TBA-40FR, parameter setting is as follows, wavelength 546nm, and the Direction of Reaction is positive direction, sample size: reagent 1: reagent 2(embodiment 2
Reagent or control)=3 μ L:200 μ L:50 μ L.2 reagent of embodiment and contrast agent are respectively divided into 6 bottles, every bottle is 2L, in room
It is detected after placing 0 month, 3 months, 6 months, 9 months, 12 months, 15 months under warm environment respectively, to Landau quality-control product
Detection 3 times is repeated, calculates separately the difference of measured value Yu quality-control product target value, the ratio of difference and target value is its relative deviation (CV),
The results are shown in Table 5.
Table 5
Its relative deviation is calculated, deviation is smaller, illustrates that reagent stability is better.According to table 5 the results show that reagent of the present invention in
After placing 15 months in room temperature environment, the accuracy for detecting sample is still good, illustrates the excellent in stability of reagent of the present invention,
The quadratic convergence polymerization of latex microsphere will not influence reagent stability.
The above embodiment is only the preferred embodiment of the present invention, cannot be limited the scope of protection of the present invention with this,
The variation and replacement for any unsubstantiality that those skilled in the art is done on the basis of the present invention belong to institute of the present invention
Claimed range.
Claims (10)
1. a kind of cystatin C detection kit based on latex immunoturbidimetry, it is characterised in that: including reagent R1 and reagent
R2, the reagent R1 include inorganic salts 0.1-120g/L, buffer 20-200 mmol/L, increasing milk agent 0.8mmol/L-4.2
Mmol/L, preservative 0.8-3.2g/L, stabilizer 1-200g/L；The reagent 2 includes buffer 20-200 mmol/L, increases cream
Agent 0.8mmol/L-4.2 mmol/L, surfactant 1-20g/L, stabilizer 1-200g/L, it is coated with cystatin C antibody sensitized
Latex particle 0.1-3g/L, preservative 0.5-5g/L, surplus is water.
2. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 1, feature exist
In: it is 0.1-1.5g/L magnesium chloride and calcium chloride or 15-120g/L sodium chloride that inorganic salts, which are by melting concn, in the reagent R1.
3. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 1, feature exist
In: the buffer is one of trihydroxy methyl amino buffer, phosphate buffer or glycine buffer；The anti-corrosion
Agent is one of Sodium azide or PC300；The stabilizer is one of trehalose, sucrose, glycine and brij-35 or more
Kind is composed；The increasing milk agent is PEG2000~PEG20000.
4. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 3, feature exist
In: the buffer is trihydroxy methyl amino buffer；The preservative is PC300；The stabilizer is trehalose；The increasing
Emulsion is PEG6000.
5. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 1, feature exist
In: the surfactant is one of polysorbas20, polysorbate60, Tween 80 and Triton X-100.
6. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 1, feature exist
In: coated on the latex particle of the sensitization is that the anti-human cystatin C of animal is polyclonal or monoclonal antibody.
7. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 1, feature exist
In: the latex particle of the sensitization is the polystyrene latex microspheres after quadratic convergence polymerization.
8. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 7, feature exist
In: quadratic convergence polymerization latex microsphere preparation method the following steps are included:
Use the method for high speed refrigerated centrifuge that latex microsphere solution replacement for distilled water, is placed in reaction kettle；
Mixed emulsifier and regulator is added, is passed through N2Replace O2Afterwards, initiator is added, causes after polymerization；
The buffer that emulsion reagent label reaction needs is added to be centrifuged.
9. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 8, feature exist
Be in: the mixed emulsifier is 1:1SDS and NP-40 in mass ratio；The regulator is lauryl mercaptan；It is described to draw
Hair agent is over cure potassium.
10. a kind of cystatin C detection kit based on latex immunoturbidimetry according to claim 8, feature exist
In: 0.72 times for completing partial size when the latex microsphere partial size of quadratic convergence polymerization reaction is unpolymerized.
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| CN109813908A (en) * | 2018-12-29 | 2019-05-28 | 宁波普瑞柏生物技术股份有限公司 | Eliminate the method and kit of hook effect in cystatin C detection |
| CN111551744A (en) * | 2020-05-15 | 2020-08-18 | 安徽中起生物科技有限公司 | Newcastle disease virus N protein IgY antibody colloidal carbon detection test paper and application thereof |
| CN112903994A (en) * | 2021-01-20 | 2021-06-04 | 南京立顶医疗科技有限公司 | High-linearity human serum amyloid kit, preparation method and application |
| CN114217071A (en) * | 2021-12-01 | 2022-03-22 | 柏荣诊断产品（上海）有限公司 | High-specificity transmission-scattering integrated ferritin latex turbidimetric detection kit |
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| CN107894509A (en) * | 2017-10-20 | 2018-04-10 | 柏荣诊断产品（上海）有限公司 | A kind of method for improving latex immunoturbidimetry antigen excess and the range of linearity |
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| CN106932594A (en) * | 2017-03-14 | 2017-07-07 | 吉林省富生医疗器械有限公司 | A kind of preferred cystatin C detection kit and preparation method thereof |
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| CN109813908A (en) * | 2018-12-29 | 2019-05-28 | 宁波普瑞柏生物技术股份有限公司 | Eliminate the method and kit of hook effect in cystatin C detection |
| CN111551744A (en) * | 2020-05-15 | 2020-08-18 | 安徽中起生物科技有限公司 | Newcastle disease virus N protein IgY antibody colloidal carbon detection test paper and application thereof |
| CN112903994A (en) * | 2021-01-20 | 2021-06-04 | 南京立顶医疗科技有限公司 | High-linearity human serum amyloid kit, preparation method and application |
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