CN106932594A - A kind of preferred cystatin C detection kit and preparation method thereof - Google Patents
A kind of preferred cystatin C detection kit and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to a kind of cystatin C detection kit and preparation method thereof, the kit includes that reagent R1 independent of each other and reagent R2, the reagent R1 are mainly made up of buffer solution 1, inorganic ion 1, stabilizer 1, preservative 1, solubilizer 1 and accelerator 1；The reagent R2 is mainly made up of the polystyrene microsphere of crosslinking bladder chalone C antibody, buffer solution 2, stabilizer 2, solubilizer 2, preservative 2 and protective agent 2; the polystyrene microsphere is connected with bladder chalone C antibody by physical absorption or chemical crosslinking, and is included by beta cyclodextrin.Cystatin C detection kit of the present invention has preparation cost cheap, has good stability, it is easy to store, the advantage that data accuracy is good and detection sensitivity is high, can be widely applied to clinical biochemical instrument.
Description
Technical field
The invention belongs to biomedicine technical field, and in particular to a kind of preferred cystatin C detection kit and its preparation
Method.
Background technology
Kidney trouble is a kind of clinically common disease, so far, early diagnosis, early treatment and reverses the kidney for damaging
Function is the approach that can uniquely prevent kidney trouble from deteriorating.Clinical evaluation kidney trouble is in progress and the order of severity is general with renal function
Be reference, using glomerular filtration rate(GFR (GFR) as reflection the most important index of renal function.
Bladder chalone C, is a kind of cystatin, also referred to as γ-trace of albumin or γ-postglobulin,
And be a kind of low-molecular-weight, alkaline non-glycated protein, its molecular weight is 13.3KD, is made up of 122 amino acid residues, can be by
The all karyocytes of body are produced, and generation rate is constant.Bladder chalone C in circulation is only eliminated through glomerular filtration, is reflection
The endogenous mark of glomerular filtration rate(GFR change, and in proximal convoluted tubule reabsorption, but by complete metabolic breakdown after reabsorption, no
Return to blood.Therefore, it can reflect by the concentration of bladder chalone C in blood the filtering rate of glomerulus.And, bladder chalone C
Serum-concentration is not influenceed by quick disease process, sex, age and muscle weight, is the preferable homologous of reflection glomerular filtration rate(GFR change
Property mark, and progressively develop into that a sensitiveness of assessment renal function is good, specificity index high.
At present, the clinically main detection for relying on immunological method to complete bladder chalone C, by the specificity knot of antigen-antibody
Close, in the way of double antibodies sandwich in quantitative determination serum bladder chalone C content.Specific detection method includes again：Enzyme linked immunological
Adsorption measurement, chemoluminescence method and immunoturbidimetry.Above two methods due to it is cumbersome, time-consuming and expense is high etc.
Deficiency, seriously limits its application.As the popularization of full automatic biochemical apparatus and various tachysynthesises are than the development of turbid detection technique, exempt from
Epidemic disease turbidimetry has that the reaction time is short concurrently, and precision is good, and detection range is wide, and the influence of jaundice, haemolysis and piarhemia sample is small, it is easy to automatic
The advantages such as change, it has also become the main flow detection method of clinical bladder chalone C.
But, because the Cleaning Principle of immunoturbidimetry lacks enzymatic iodine, cause the immune ratio of existing bladder chalone C
Inevitably there is the more low deficiency of sensitivity in turbid detection kit.Meanwhile, in kit preparation process, to ensure product
Required sensitivity is reached, compared with enzyme-linked immunosorbent assay and chemoluminescence method, immunoturbidimetry will to the quality of antibody used
Ask higher, package amount and antibody usage amount are bigger, also result in the increase of kit cost.And macromolecule glue is widely used at present
The surface-crosslinked monoclonal antibody of newborn microballoon, after the microballoon of antibody is crosslinked with antigen binding, can gather rapidly in a short time
Gather together, change the astigmatism performance or light transmission of reaction solution.The preparation of existing cystatin C detection kit there is also
Complex operation, time-consuming, to shortcoming that the is limitation of operator's professional skill and cannot ensureing product stability and accuracy.
The content of the invention
The purpose of the present invention is that for above-mentioned the deficiencies in the prior art, there is provided a kind of preferred bladder chalone C detection reagent
Box, the kit can further improve the stability of bladder chalone C detection and accurate compared with existing cystatin C detection kit
Property, and cost is lower.
It is a further object of the present invention to provide the preparation method of above-mentioned preferred cystatin C detection kit, the method operation
Simply, it is easy to promote the use of.
The purpose of the present invention is achieved through the following technical solutions：
A kind of preferred cystatin C detection kit, including reagent R1 independent of each other and reagent R2, the reagent R1 is main
It is made up of buffer solution 1, inorganic ion 1, stabilizer 1, preservative 1, solubilizer 1 and accelerator 1；The reagent R2 is main by handing over
Polystyrene microsphere, buffer solution 2, stabilizer 2, solubilizer 2, preservative 2 and the protective agent 2 for joining bladder chalone C antibody are constituted, and are protected
Shield agent 2 is beta-schardinger dextrin；
The polystyrene microsphere is connected or Surfaces of Polystyrene Microparticles ammonia with bladder chalone C antibody by physical absorption
Base, carboxyl, hydrazides or epoxy radicals and bladder chalone C antibody surface amino covalence are coupled, and by beta-schardinger dextrin (protective agent 2) bag
Close, polystyrene microsphere particle diameter is 100nm.
Preservative 1 and 2 in the technical program refers to the class reagent that can suppress bacterium and microorganism pollution in reagent,
There is the effect of antisepsis and sterilization to reagent.Protective agent 2 in reagent R2 is the antibody that a class can protect latex particle surface
Reagent.Inorganic ion 1 can keep the charge balance in reagent R1.
Preferred cystatin C detection kit in the technical program, bladder chalone C is antibody linked in polystyrene microsphere table
Face, and by beta-cyclodextrin inclusion compound, when the bladder chalone C in blood and bladder chalone C antibody response, drive polystyrene microsphere aggregation
Certain turbidity is produced, and turbidity is directly proportional to the bladder chalone C content in blood in certain limit, can be existed with full automatic biochemical apparatus
The content of bladder chalone C in blood is detected under 400-800nm wavelength.
Preferably, the composition of above-mentioned preferred cystatin C detection kit is as follows：
Reagent R1, its component is：
Reagent R2, its component is：
It is further preferred that the concrete composition of above-mentioned preferred cystatin C detection kit is as follows：
Reagent R1, its component is：
Reagent R2, its component is：
Preferably, the buffer solution 1 in the reagent R1 is selected from Hepes buffer solutions, Tris-HCl buffer solutions, MOPS bufferings
The combination of any one or more in liquid, phosphate buffer, borate buffer solution, its pH is 7.0~9.0；The reagent R2
In buffer solution 2 be selected from phosphate buffer, borate buffer solution, Hepes buffer solutions, Tris-HCl buffer solutions, MOPS buffer solutions
In the combination of any one or more, its pH be 7.0~9.0.
Preferably, the stabilizer 1 in the reagent R1 is selected from bovine serum albumin(BSA), mannose, sorbierite, ethylenediamine tetrem
One or more in acid disodium, fructose of combination；Stabilizer 2 in the reagent R2 be selected from bovine serum albumin(BSA), mannose,
One or more in sorbierite, disodium ethylene diamine tetraacetate, fructose of combination.
Preferably, the inorganic ion 1 in the reagent R1 is selected from any one in potassium chloride, sodium chloride, calcium chloride
Or various combinations, have the advantages that cheap, raw material is easy to get.
Preferably, the preservative 1 in the reagent R1 is Sodium azide, thimerosal or ProClin300；The reagent R2
In preservative 2 be Sodium azide, thimerosal or ProClin300.Preservative 1 and 2 has excellent antisepsis and sterilization performance.
Preferably, the accelerator 1 in the reagent R1 be Polyethylene glycol-2000, PEG-4000, polyethylene glycol-
8000th, the combination of one or more in polyvinylpyrrolidone.
Preferably, the solubilizer 1 in the reagent R1 and reagent R2 is polysorbas20.
Also include the calibration object of bladder chalone C reagent, calibration object is that bladder chalone C content is 0.1mg/L, 0.5mg/L, 1.0mg/
Six gradient standard items of L, 2.0mg/L, 4.0mg/L and 8.0mg/L, solution is bovine serum albumin(BSA) 10g/L, tween
200.3mmol/L, Sodium azide 10mmol/L, the buffer solution of phosphate buffer 1 0mmol/L.
The preparation method of preferred cystatin C detection kit, comprises the following steps as described above：
(1), the preparation of reagent R1：
Prepare buffer solution 1：5~15mmol/L of phosphate buffer；
5~15g/L of bovine serum albumin(BSA), 0.1~0.3mmol/L of polysorbas20, polyethylene glycol 2.0 are added in buffer solution 1
~5.0mmol/L, 0.08~0.16mol/L of sodium chloride, 5~15mmol/L of Sodium azide, are uniformly mixed, and obtain final product reagent R1；
(2), the preparation of reagent R2：
Step one：Bladder chalone C antibody is carried out into 4 DEG C of dialysis, then is diluted to bladder chalone C antibody with phosphate buffer
0.05~5mg/ml, obtains bladder chalone C antibody diluent；Polystyrene microsphere is centrifuged with purified water, is washed 3 times；
Step 2：The polystyrene microsphere after step one is washed is diluted into mass concentration with phosphate buffer is
0.1%~3%, mass concentration is added for 0.1~0.5% beta-schardinger dextrin, reaction 30min is stirred at room temperature, reaction terminates
15000rpm centrifuge washings are subsequently adding the bladder chalone C antibody dilution that step one is obtained to remove unreacted beta-schardinger dextrin afterwards
Liquid, stirring reaction 30min, adds terminate liquid terminating reaction at room temperature, the reaction solution 15000rpm centrifugations that will be obtained, and uses phosphoric acid
W salt buffer washes are precipitated, and repeated centrifugation is washed 3 times, is eventually adding 5~15mmol/L of phosphate buffer, bovine serum albumin(BSA)
5~15g/L, 0.1~0.5mmol/L of polysorbas20,5~15mmol/L of Sodium azide are uniformly mixed and obtain final product reagent R2；
(3), the preparation of calibration object：Bladder chalone C content as required be 0.1mg/L, 0.5mg/L, 1.0mg/L,
Six gradient standard items of 2.0mg/L, 4.0mg/L and 8.0mg/L, corresponding bladder chalone C standard items are separately added into containing ox blood
In pure protein 10 g/L, polysorbas20 0.3mmol/L, Sodium azide 10mmol/L, phosphate buffer 1 0mmol/L buffer solutions.
Preferably, the preparation method of above-mentioned preferred cystatin C detection kit, specifically includes following steps：
(1), the preparation of reagent R1：
Prepare buffer solution 1：Phosphate buffer 1 0mmol/L；
Added in buffer solution 1 bovine serum albumin(BSA) 10g/L, polysorbas20 0.2mmol/L, polyethylene glycol 3.5mmol/L,
Sodium chloride 0.12mol/L, Sodium azide 10mmol/L, are uniformly mixed, and obtain final product reagent R1；
(2), the preparation of reagent R2：
Step one：Bladder chalone C antibody is carried out into 4 DEG C of dialysis, then is diluted to bladder chalone C antibody with phosphate buffer
2mg/ml, obtains bladder chalone C antibody diluent；Polystyrene microsphere is centrifuged with purified water, is washed 3 times；
Step 2：The polystyrene microsphere after step one is washed is diluted into mass concentration with phosphate buffer is
1.0%, mass concentration is added for 0.3% beta-schardinger dextrin, reaction 30min is stirred at room temperature, reaction terminates rear 15000rpm
Centrifuge washing is subsequently adding the bladder chalone C antibody diluent that step one is obtained to remove unreacted beta-schardinger dextrin, stirs at room temperature
Reaction 30min is mixed, terminate liquid terminating reaction is added, the reaction solution 15000rpm centrifugations that will be obtained are washed with phosphate buffer
Precipitation is washed, repeated centrifugation is washed 3 times, is eventually adding phosphate buffer 1 0mmol/L, bovine serum albumin(BSA) 10g/L, polysorbas20
0.3mmol/L, Sodium azide 10mmol/L are uniformly mixed and obtain final product reagent R2；
(3), the preparation of calibration object：Bladder chalone C content as required be 0.1mg/L, 0.5mg/L, 1.0mg/L,
Six gradient standard items of 2.0mg/L, 4.0mg/L and 8.0mg/L, corresponding bladder chalone C standard items are separately added into containing ox blood
In pure protein 10 g/L, polysorbas20 0.3mmol/L, Sodium azide 10mmol/L, phosphate buffer 1 0mmol/L buffer solutions.
Compared with existing detection technique, beneficial effects of the present invention are：
1st, polystyrene microsphere be connected with bladder chalone C antibody by physical absorption or the amino by its surface, carboxyl,
Amino of the functional group such as hydrazides or epoxy radicals and antibody surface etc. combines to form covalent coupling structure, makes bladder chalone C antibody jail
Solid combination in microsphere surface.Further by the inclusion of beta-schardinger dextrin, it is ensured that the stability of reagent R2, reagent is extended
The R2 terms of validity.
2nd, it is the bulky grain polystyrene microsphere particle of 100nm to use particle diameter, and with beta-cyclodextrin inclusion compound, make it have compared with
Big particle diameter, kit while with higher sensitivity, compared to the stability that available reagent box not only increases reagent, and
And turbidity when increased bladder chalone C and bladder chalone C antibody response in blood, so as to improve the accuracy of test reaction and anti-
Interference performance, and shorten the reaction time, it is ensured that test result stabilization, reliability.
3rd, using beta-schardinger dextrin as protective agent 2, not only cause that kit has more preferably sensitivity and color developing effect, also
So that the term of validity is extended compared with available reagent box.Kit is stored in 2-8 DEG C, in light protected environment, the term of validity is 24
Month；Uncap and be stored in 2-8 DEG C, in light protected environment, the term of validity is 90 days.Available reagent box and unused beta-schardinger dextrin are made on year-on-year basis
For protectant kit term of validity rises appreciably, it is more convenient for depositing and uses.
4th, kit low cost, preparation method is simple to operate, it is easy to promote the use of.
Specific embodiment
A kind of preferred cystatin C detection kit, including reagent R1 independent of each other and reagent R2, the reagent R1 is main
It is made up of buffer solution 1, inorganic ion 1, stabilizer 1, preservative 1, solubilizer 1 and accelerator 1；The reagent R2 is main by handing over
Polystyrene microsphere, buffer solution 2, stabilizer 2, solubilizer 2, preservative 2 and the protective agent 2 for joining bladder chalone C antibody are constituted, and are protected
Shield agent 2 is beta-schardinger dextrin；
The polystyrene microsphere is connected or Surfaces of Polystyrene Microparticles ammonia with bladder chalone C antibody by physical absorption
Base, carboxyl, hydrazides or epoxy radicals and bladder chalone C antibody surface amino covalence are coupled, and by beta-schardinger dextrin (protective agent 2) bag
Close, polystyrene microsphere particle diameter is 100nm.
Preferably, the buffer solution 1 in the reagent R1 is selected from Hepes buffer solutions, Tris-HCl buffer solutions, MOPS bufferings
The combination of any one or more in liquid, phosphate buffer, borate buffer solution, its pH is 7.0~9.0；The reagent R2
In buffer solution 2 be selected from phosphate buffer, borate buffer solution, Hepes buffer solutions, Tris-HCl buffer solutions, MOPS buffer solutions
In the combination of any one or more, its pH be 7.0~9.0.
Preferably, the stabilizer 1 in the reagent R1 is selected from bovine serum albumin(BSA), mannose, sorbierite, ethylenediamine tetrem
One or more in acid disodium, fructose of combination；Stabilizer 2 in the reagent R2 be selected from bovine serum albumin(BSA), mannose,
One or more in sorbierite, disodium ethylene diamine tetraacetate, fructose of combination.
Preferably, the inorganic ion 1 in the reagent R1 is selected from any one in potassium chloride, sodium chloride, calcium chloride
Or various combinations, have the advantages that cheap, raw material is easy to get.
Preferably, the preservative 1 in the reagent R1 is Sodium azide, thimerosal or ProClin300；The reagent R2
In preservative 2 be Sodium azide, thimerosal or ProClin300.Preservative 1 and 2 has excellent antisepsis and sterilization performance.
Preferably, the accelerator 1 in the reagent R1 be Polyethylene glycol-2000, PEG-4000, polyethylene glycol-
8000th, the combination of one or more in polyvinylpyrrolidone.
Preferably, the solubilizer 1 in the reagent R1 and reagent R2 is polysorbas20.
Also include the calibration object of bladder chalone C reagent, calibration object is that bladder chalone C content is 0.1mg/L, 0.5mg/L, 1.0mg/
Six gradient standard items of L, 2.0mg/L, 4.0mg/L and 8.0mg/L, solution is bovine serum albumin(BSA) 10g/L, tween
200.3mmol/L, Sodium azide 10mmol/L, the buffer solution of phosphate buffer 1 0mmol/L.
Embodiment 1
Kit citing of the invention is double reagent, wherein：
Reagent R1
Reagent R2：
Embodiment 2
Preferably, the invention discloses a kind of preferred bladder chalone C determining reagent kit, including reagent R1 independent of each other and
Reagent R2 constitute kit, including composition and corresponding content be：
It is coated with bladder chalone C antibody polystyrene microsphere and beta-schardinger dextrin particle, particle diameter 100nm, concentration 1%.
Calibration object：
Corresponding bladder chalone C standard items are separately added into above-mentioned buffering by bladder chalone C reference calibrations product concentration as required
Liquid, prepares one group of calibration object of various concentrations.
Embodiment 3
Kit application method：
1st, reagent prepares, and reagent is liquid double reagent, and corkage is used, wherein：
(1), the preparation of reagent R1：
Configuration buffer solution 1：Phosphate buffer 1 0mmol/L；
Added in buffer solution 1 bovine serum albumin(BSA) 10g/L, polysorbas20 0.2mmol/L, polyethylene glycol 3.5mmol/L,
Sodium chloride 0.12mol/L, Sodium azide 10mmol/L, are uniformly mixed, and obtain final product reagent R1；
(2), the preparation of reagent R2：
Step one：Bladder chalone C antibody is carried out into 4 DEG C of dialysis, then is diluted to bladder chalone C antibody with phosphate buffer
2mg/ml, obtains bladder chalone C antibody diluent；Polystyrene microsphere is centrifuged with purified water, is washed 3 times；
Step 2：The polystyrene microsphere after step one is washed is diluted into mass concentration with phosphate buffer is
1.0%, mass concentration is added for 0.3% beta-schardinger dextrin, reaction 30min is stirred at room temperature, reaction terminates rear 15000rpm
Centrifuge washing is subsequently adding the bladder chalone C antibody diluent that step one is obtained to remove unreacted beta-schardinger dextrin, stirs at room temperature
Reaction 30min is mixed, terminate liquid terminating reaction is added, the reaction solution 15000rpm centrifugations that will be obtained are washed with phosphate buffer
Precipitation is washed, repeated centrifugation is washed 3 times, is eventually adding phosphate buffer 1 0mmol/L, bovine serum albumin(BSA) 10g/L, polysorbas20
0.3mmol/L, Sodium azide 10mmol/L are uniformly mixed and obtain final product reagent R2；
(3), the preparation of calibration object：Bladder chalone C content as required is 0.1,0.5,1.0,2.0,4.0 and 8.0mg/L
Six gradient standard items, add 0.3mmol/L containing polysorbas20, Sodium azide 10mmol/L, phosphate buffer 1 0mmol/L slow
In fliud flushing.
2nd, full automatic biochemical apparatus parameter setting
(a), Detection wavelength：Dominant wavelength is 546nm, a length of 700nm of complementary wave；
(b), detection temperature：37℃；
(c), the reaction time：10min, wherein, incubation time 5min determines reading absorbance immediately after adding reagent R2
A1, reads absorbance A 2 after 5 minutes, calculate absorbance change.
Δ A=A2-A1；
(d) the Direction of Reaction：Negative direction.
3rd, detecting step
(a), take 200ul reagent R1 and 5ul serum sample (avoiding haemolysis) mixings；
(b), by the solution after mixing in 37 DEG C of incubation time 5min；
(c), 50ul reagent R2 are added, reading absorbance A is determined immediately absorbance A 2 is read after 1,5 minutes, calculated and inhale
Light varience Δ A=A2-A1.
4th, the content of bladder chalone C bladder chalone C in sample is calculated by calibration curve.
Following tests are the performance test to the kit of embodiment 2
Cystatin C detection kit prepared by embodiment 2 carries out performance test, mainly tests its sensitivity for analysis, most
Low detection limits, precision, accuracy, stability and anti-interference etc..
1) sensitivity：Sensitivity technique result is as follows：
2) LDL：Using 10g/L bovine serum albumin(BSA)s normal saline solution as dummy, dummy should
Without measured object.Detection 20 times is continuously repeated on Biochemical Analyzer, testing result is recorded.Result shows that its lowest detection is limited to
0.007ug/L。
3) detect that the human serum sample of basic, normal, high 3 bladder chalone C contents of value is each with the bladder chalone C kit in the present invention
20 times, determine the withinrun precision of detection kit of the present invention.1 human serum sample is determined respectively using 3 lot number kits
20 times, calculate detection kit of the present invention batch between with respect to extreme difference.Result shows, the withinrun precision of detection kit of the present invention
It is preferable with betweenrun precision.
The withinrun precision result of the kit of preparation
| Sample 1 | Sample 2 | Sample 3 | |
| Determine number of times | 20 | 20 | 20 |
| Average value (mg/L) | 0.51 | 3.82 | 5.04 |
| Minimum value (mg/L) | 0.50 | 3.78 | 4.97 |
| Maximum (mg/L) | 0.53 | 3.84 | 5.11 |
| Standard deviation (SD) | 0.005 | 0.04 | 0.06 |
| The coefficient of variation (CV%) | 0.98 | 1.05 | 1.19 |
The betweenrun precision result of the kit of preparation
Kit withinrun precision (CV% is between 0.99~1.2) and betweenrun precision (with respect to extreme difference % 0.9~
Between 1.3) show good, data are not listed one by one herein.
4) accuracy：The conventional detection sample of suitable concn is selected, to adding different amounts of numeraire in conventional sample
Product are fabricated to recovery sample, and definite value sample purified water is used as solvent；The purified water of same amount is added to make in conventional sample
Into basic sample, the amount of numeraire product is added no more than the 1/10 of cumulative volume, each is repeated 3 times detection and takes its average to reclaim
Concentration.Result display average recovery rate is 99.86%, and the degree of accuracy is higher.
5) stability：Taking cystatin C detection kit carries out stability test, 2-8 DEG C place respectively temporally 0,3,6,
Detected within 9,12,13,14,15,16,17,18,19,20,21,22,23,24 months；Stability test uncap respectively by 2-8
DEG C place 0 day, 7 days, 14 days, 16 days, 18 days, 20 days, 22 days, 24 days, 26 days, 28 days, 30 days, 32 days, 34 days, 36 days, 38
My god, 40 days, 42 days, 44 days, 46 days, 48 days, 50 days, 52 days, 54 days, 56 days, 58 days, 60 days, 62 days, 64,66,68,70,
72nd, it is measured within 74,76,78,80,82,84,86,88 and 90 days.Result display cystatin C detection kit is stored in 2-8
DEG C, in light protected environment, the term of validity is 24 months.Uncap and be stored in 2-8 DEG C, in light protected environment, the term of validity is 90 days.
6) anti-interference：The chaff interference of various concentrations is added in serum specimen, control group adds purified water, to add
Three detection average values of serum of interfering material, as measured value, to add three detections of control group of serum of purified water flat
It is worth on the basis of average.Measured value subtracts a reference value and then obtains deviation divided by a reference value.Result display sample mesobilirubin≤300 μ
Interference when mol/L, hemoglobin≤1mg/mL, chyle≤0.30% to cystatin C detection kit testing result is less than
9.7%.
Embodiment 4
The preparation of contrast agent box and use：
Buffer solution 1 in reagent R1 uses phosphate buffer 1 0mmol/L, and other reagents and experimental technique are with implementation
Example 2.
It is coated with bladder chalone C antibody polystyrene microsphere particle, particle diameter 100nm, concentration 1%.
Calibration object：
According to 0.1mg/L, six gradient standard items of 0.5mg/L, 1.0mg/L, 2.0mg/L, 4.0mg/L and 8.0mg/L,
Corresponding bladder chalone C standard items are separately added into above-mentioned buffer solution, one group of calibration object of various concentrations is prepared.
Cystatin C detection kit prepared by embodiment 4 carries out performance test, and method is with embodiment 3.
1) LDL：Using 10g/L bovine serum albumin(BSA)s normal saline solution as dummy, dummy should
Without measured object.Detection 20 times is continuously repeated on Biochemical Analyzer, testing result is recorded.Result shows that its lowest detection is limited to
1.0g/L。
2) accuracy：The conventional detection sample of suitable concn is selected, to adding different amounts of numeraire in conventional sample
Product are fabricated to recovery sample, and definite value sample purified water is used as solvent；The purified water of same amount is added to make in conventional sample
Into basic sample, no more than the 1/10 of cumulative volume, each is repeated 3 times detection and takes its average and is back the amount of the numeraire product of addition
Receive concentration.Result display average recovery rate is 99.41%, and the degree of accuracy is higher.
3) stability：Taking the detection kit of the present embodiment carries out normal storage stability test, and 2-8 DEG C of placement is pressed respectively
Time 0,3,6,9,12,13,14 and detected for 15 months；Uncap stability test respectively by 2-8 DEG C place 0 day, 7 days, 14
My god, be measured within 16 days, 18 days, 20 days, 22 days, 24 days, 26 days, 28 days, 30 days and 32 days.Result shows the inspection of the present embodiment
Test agent box is stored in 2-8 DEG C, in light protected environment, the term of validity is 13 months.Uncap and be stored in 2-8 DEG C, in light protected environment, effectively
Phase is 25 days.
4) anti-interference：The chaff interference of various concentrations is added in serum specimen, control group adds purified water, to add
Three detection average values of serum of interfering material, as measured value, to add three detections of control group of serum of purified water flat
It is worth on the basis of average.Measured value subtracts a reference value and then obtains deviation divided by a reference value.Result display sample mesobilirubin≤300 μ
Interference when mol/L, hemoglobin≤1mg/mL, chyle≤0.30% to cystatin C detection kit testing result is less than
15%.
In sum, preferred cystatin C detection kit provided by the present invention has good anti-interference and special
Property, and with good sensitivity, accuracy, extension of validity effectively overcomes various shortcoming of the prior art and has height
Degree industrial utilization.
Claims (10)
1. a kind of preferred cystatin C detection kit, it is characterised in that：It is described including reagent R1 independent of each other and reagent R2
Reagent R1 is mainly made up of buffer solution 1, inorganic ion 1, stabilizer 1, preservative 1, solubilizer 1 and accelerator 1；The reagent
R2 is mainly by the polystyrene microsphere of crosslinking bladder chalone C antibody, buffer solution 2, stabilizer 2, solubilizer 2, preservative 2 and protective agent
2 compositions, wherein protective agent 2 are beta-schardinger dextrin；
The polystyrene microsphere is connected or Surfaces of Polystyrene Microparticles amino, carboxylic with bladder chalone C antibody by physical absorption
Base, hydrazides or epoxy radicals and bladder chalone C antibody surface amino covalence are coupled, and by beta-cyclodextrin inclusion compound, polystyrene microsphere
Particle diameter is 100nm.
2. a kind of preferred cystatin C detection kit according to claim 1, it is characterised in that composition is as follows：
Reagent R1, its component is：
Reagent R2, its component is：
3. a kind of preferred cystatin C detection kit according to claim 2, it is characterised in that concrete composition is as follows：
Reagent R1, its component is：
Reagent R2, its component is：
4. a kind of preferred cystatin C detection kit according to claim 1, it is characterised in that：In the reagent R1
Buffer solution 1 is appointing in Hepes buffer solutions, Tris-HCl buffer solutions, MOPS buffer solutions, phosphate buffer, borate buffer solution
The combination that one or more of meaning, its pH is 7.0~9.0；Buffer solution 2 in the reagent R2 is phosphate buffer, borax delays
The combination of any one or more in fliud flushing, Hepes buffer solutions, Tris-HCl buffer solutions, MOPS buffer solutions, its pH be 7.0~
9.0。
5. a kind of preferred cystatin C detection kit according to claim 1, it is characterised in that：In the reagent R1
Stabilizer 1 is the group of one or more in bovine serum albumin(BSA), mannose, sorbierite, disodium ethylene diamine tetraacetate, fructose
Close；Stabilizer 2 in the reagent R2 is in bovine serum albumin(BSA), mannose, sorbierite, disodium ethylene diamine tetraacetate, fructose
One or more of combination.
6. a kind of preferred cystatin C detection kit according to claim 1, it is characterised in that：In the reagent R1
Preservative 1 is Sodium azide, thimerosal or ProClin300；Preservative 2 in the reagent R2 is Sodium azide, thimerosal or
ProClin300。
7. a kind of preferred cystatin C detection kit according to claim 1, it is characterised in that：In the reagent R1
Inorganic ion 1 is KCl, NaCl, CaCl2, in the combination of any one or more；Accelerator 1 in the reagent R1 is
One or more in Polyethylene glycol-2000, PEG-4000, PEG-8 000, polyvinylpyrrolidone of combination；
Solubilizer 1 in the reagent R1 and reagent R2 is polysorbas20.
8. a kind of preferred cystatin C detection kit according to claim 1, it is characterised in that：Also tried including bladder chalone C
The calibration object of agent, calibration object be bladder chalone C content be 0.1mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L, 4.0mg/L and
Six gradient standard items of 8.0mg/L, solution is bovine serum albumin(BSA) 10g/L, polysorbas20 0.3mmol/L, Sodium azide
The buffer solution of 10mmol/L, phosphate buffer 1 0mmol/L.
9. a kind of preparation method of preferred cystatin C detection kit as claimed in claim 2, it is characterised in that including with
Lower step：
(1), the preparation of reagent R1：
Prepare buffer solution 1：5~15mmol/L of phosphate buffer；
Added in buffer solution 1 bovine serum albumin(BSA) 5~15g/L, 0.1~0.3mmol/L of polysorbas20, polyethylene glycol 2.0~
5.0mmol/L, 0.08~0.16mol/L of sodium chloride, 5~15mmol/L of Sodium azide, are uniformly mixed, and obtain final product reagent R1；
(2), the preparation of reagent R2：
Step one：Bladder chalone C antibody is carried out into 4 DEG C of dialysis, then bladder chalone C antibody is diluted to 0.05 with phosphate buffer~
5mg/ml, obtains bladder chalone C antibody diluent；Polystyrene microsphere is centrifuged with purified water, is washed 3 times；
Step 2：The polystyrene microsphere after step one is washed is diluted into mass concentration with phosphate buffer is
0.1%~3%, mass concentration is added for 0.1~0.5% beta-schardinger dextrin, reaction 30min is stirred at room temperature, reaction terminates
15000rpm centrifuge washings are subsequently adding the bladder chalone C antibody dilution that step one is obtained to remove unreacted beta-schardinger dextrin afterwards
Liquid, stirring reaction 30min, adds terminate liquid terminating reaction at room temperature, the reaction solution 15000rpm centrifugations that will be obtained, and uses phosphoric acid
W salt buffer washes are precipitated, and repeated centrifugation is washed 3 times, is eventually adding 5~15mmol/L of phosphate buffer, bovine serum albumin(BSA)
5~15g/L, 0.1~0.5mmol/L of polysorbas20,5~15mmol/L of Sodium azide are uniformly mixed and obtain final product reagent R2；
(3), the preparation of calibration object：Bladder chalone C content as required be 0.1mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L,
Six gradient standard items of 4.0mg/L and 8.0mg/L, corresponding bladder chalone C standard items are separately added into containing bovine serum albumin(BSA)
In 10g/L, polysorbas20 0.3mmol/L, Sodium azide 10mmol/L, phosphate buffer 1 0mmol/L buffer solutions.
10. a kind of preparation method of preferred cystatin C detection kit as claimed in claim 9, it is characterised in that specific bag
Include following steps：
(1), the preparation of reagent R1：
Prepare buffer solution 1：Phosphate buffer 1 0mmol/L；
Bovine serum albumin(BSA) 10g/L, polysorbas20 0.2mmol/L, polyethylene glycol 3.5mmol/L, chlorination are added in buffer solution 1
Sodium 0.12mol/L, Sodium azide 10mmol/L, are uniformly mixed, and obtain final product reagent R1；
(2), the preparation of reagent R2：
Step one：Bladder chalone C antibody is carried out into 4 DEG C of dialysis, then bladder chalone C antibody is diluted to 2mg/ with phosphate buffer
Ml, obtains bladder chalone C antibody diluent；Polystyrene microsphere is centrifuged with purified water, is washed 3 times；
Step 2：The polystyrene microsphere after step one is washed is diluted into mass concentration with phosphate buffer is
1.0%, mass concentration is added for 0.3% beta-schardinger dextrin, reaction 30min is stirred at room temperature, reaction terminates rear 15000rpm
Centrifuge washing is subsequently adding the bladder chalone C antibody diluent that step one is obtained to remove unreacted beta-schardinger dextrin, stirs at room temperature
Reaction 30min is mixed, terminate liquid terminating reaction is added, the reaction solution 15000rpm centrifugations that will be obtained are washed with phosphate buffer
Precipitation is washed, repeated centrifugation is washed 3 times, is eventually adding phosphate buffer 1 0mmol/L, bovine serum albumin(BSA) 10g/L, polysorbas20
0.3mmol/L, Sodium azide 10mmol/L are uniformly mixed and obtain final product reagent R2；
(3), the preparation of calibration object：Bladder chalone C content as required be 0.1mg/L, 0.5mg/L, 1.0mg/L, 2.0mg/L,
Six gradient standard items of 4.0mg/L and 8.0mg/L, corresponding bladder chalone C standard items are separately added into containing bovine serum albumin(BSA)
In 10g/L, polysorbas20 0.3mmol/L, Sodium azide 10mmol/L, phosphate buffer 1 0mmol/L buffer solutions.
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| CN108107201A (en) * | 2017-12-20 | 2018-06-01 | 青岛汉唐生物科技有限公司 | A kind of cystatin C detection kit and preparation method thereof |
| CN108872604A (en) * | 2018-07-27 | 2018-11-23 | 金华市强盛生物科技有限公司 | A kind of cystatin C detection kit |
| CN108931648A (en) * | 2018-07-18 | 2018-12-04 | 沈阳医陆生物科技有限公司 | A kind of cystatin C detection kit based on latex immunoturbidimetry |
| CN109613237A (en) * | 2018-12-28 | 2019-04-12 | 上海高踪医疗器械科技有限公司 | A kind of cystatin C detection kit |
| CN110846303A (en) * | 2019-11-23 | 2020-02-28 | 吉林省富生医疗器械有限公司 | Peroxidase protective agent |
| CN113687075A (en) * | 2021-09-18 | 2021-11-23 | 北京安图生物工程有限公司 | Ceruloplasmin detection kit and preparation method thereof |
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| CN108107201A (en) * | 2017-12-20 | 2018-06-01 | 青岛汉唐生物科技有限公司 | A kind of cystatin C detection kit and preparation method thereof |
| CN108931648A (en) * | 2018-07-18 | 2018-12-04 | 沈阳医陆生物科技有限公司 | A kind of cystatin C detection kit based on latex immunoturbidimetry |
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| CN113687075A (en) * | 2021-09-18 | 2021-11-23 | 北京安图生物工程有限公司 | Ceruloplasmin detection kit and preparation method thereof |
| CN113687075B (en) * | 2021-09-18 | 2024-03-12 | 北京安图生物工程有限公司 | Ceruloplasmin detection kit and preparation method thereof |
| CN117783541A (en) * | 2023-12-30 | 2024-03-29 | 中拓生物有限公司 | Directional coupling cystatin C determination kit and preparation method and application thereof |
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