CN108835428A - A kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation method - Google Patents

A kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation method Download PDF

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CN108835428A
CN108835428A CN201810927798.2A CN201810927798A CN108835428A CN 108835428 A CN108835428 A CN 108835428A CN 201810927798 A CN201810927798 A CN 201810927798A CN 108835428 A CN108835428 A CN 108835428A
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feed
growing
fattening pigs
full price
leavening
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史新娥
赵博
李晓
韩启春
卢军锋
樊路杰
谢宝财
张旋
马国际
陶泽宇
孙超
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Northwest A&F University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/30Feeding-stuffs specially adapted for particular animals for swines
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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Abstract

The present invention provides a kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation methods.Preparation method of the invention includes:Fermentation prepares fermented feed under closed environment after bacterium solution and basal feed are mixed, and the bacterium solution is formulated by leavening and water of the invention;The basal feed is formulated by corn, dregs of beans, wheat bran and premix.Simultaneously the present invention provides leavening, which includes lactobacillus fermenti, Bifidobacterium, clostridium butyricum and streptococcus thermophilus.The present invention is after bacterium solution is fermented, and the nutriments such as crude protein, crude fat, coarse ash are decomposed in feed, and the constituent contents such as calcium, phosphorus, which also have, largely to be changed, to play the effect for promoting growth of animal, maintaining animal body health.

Description

A kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation method
Technical field
The invention belongs to field of biotechnology, are related to the production of animal fermented feed, in particular to a kind of growing and fattening pigs full price Fermentative feedstuff of microbe preparation method.
Background technique
China is Feed Manufacturing big country of the world, and wherein pannage accounting is 45%.The quality of pannage directly influences pig Meat, and then influence the health of human body.However existing feed feeding pig has a disadvantage that:Palatability is poor, feed cost compared with The growth performance of height, pig is more low.
Summary of the invention
For the defects or inadequacies for existing technologies, present invention firstly provides a kind of growing and fattening pigs full price fermentative feedstuff of microbe Leavening is used in preparation.
Leavening provided by the invention includes lactobacillus fermenti, Bifidobacterium, clostridium butyricum and streptococcus thermophilus.
Dosage relation is between each component in leavening of the invention:1kg powder starter by following mass percent component It is made:Lactobacillus fermenti 63.18%, clostridium butyricum 23.70%, Bifidobacterium 10.56%, streptococcus thermophilus 2.56%.
Further, the present invention provides a kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation methods.Preparation of the invention Method includes:Fermentation prepares fermented feed under closed environment after bacterium solution and basal feed are mixed, and the bacterium solution is by the present invention Leavening and water be formulated;The basal feed is formulated by corn, dregs of beans, wheat bran and premix.
Preferably, the basal feed by mass percent be 67% corn, 21% dregs of beans, 8% wheat bran and 4% Premix be formulated;The bacterium solution adds the ratio of 1kg bacterium powder to be formulated by every 40L water;The basal feed and bacterium solution Dosage relation be:Basal feed per ton adds 400L to dilute bacterium solution.
Further, it is preferred that 30~35 DEG C of fermentation temperature in preparation method of the present invention fermented through 5~7 days, fermented Feed.
The present invention is after bacterium solution is fermented, and the nutriments such as crude protein, crude fat, coarse ash are decomposed in feed, calcium phosphorus etc. Constituent content, which also has, largely to be changed, to play the effect for promoting growth of animal, maintaining animal body health.Wherein ferment Lactobacillus has the function of promoting growth of animal and prevents diarrhea;Bifidobacterium has a variety of prebiotic functions, including improves animal Immune performance, inhibit pathogen intrusion enteron aisle, alleviate body oxidative damage, improve because of disease caused by intestinal bacilli illness Deng to promote the health of animal body.
Specific embodiment
Lactobacillus fermenti, clostridium butyricum used in the present invention, Bifidobacterium and streptococcus thermophilus are commercial product.Such as table Product type shown in 1.
Corn, dregs of beans used in the present invention, wheat bran and premix are the feed of this field routine.Premix therein is commercially available Product, for example, the s414 premix of Da Bei Nong group production and the similar functions product of other relevant enterprises production.
Fermentation of the present invention for growing and fattening pigs YH bacterium full price fermentative feedstuff of microbe preparation bacterium solution and feeding growing and fattening pigs is raised Material, should prepare in time, be made within 2 days preferably before feeding.
Below in conjunction with specific embodiment, the present invention is further described in detail.
Below in an example, the preparation of growing and fattening pigs YH bacterium full price fermentative feedstuff of microbe uses bacterium solution and feeds growing and fattening pigs Fermented feed need to be made for 2 days before feeding.
(1) leavening of pregnant sow full price fermentative feedstuff of microbe
The present embodiment provides growing and fattening pigs full price fermentative feedstuff of microbe preparation leavening.
1kg YH bacterium powder includes following substance:Lactobacillus fermenti 63.18%, clostridium butyricum 23.70%, Bifidobacterium 10.56%, streptococcus thermophilus 2.56%.Lactobacillus fermenti used in the embodiment, Bifidobacterium, clostridium butyricum and streptococcus thermophilus It is purchased from China General Microbiological culture presevation administrative center (table 1).
Strain list used in 1 embodiment of table
(2) pregnant sow matches the preparation of combined bacteria full price fermentative feedstuff of microbe
This example provides the growing and fattening pigs full price fermentative feedstuff of microbe of the leavening preparation using embodiment, and preparation method is such as Under:
(1) raw material, which is prepared, crushes:Feed is configured, wherein corn 67%, dregs of beans 21%, wheat bran 8%, (the big north of premix 4% The s414 premix of agriculture group production), raw material is crushed, is stirred.
(2) bacterium solution is prepared:Add 1kg bacterium powder ratio to be added to the water with every 40L water in bacterium powder to stir evenly.
(3) under blender cooperation, bacterium solution will be prepared and be slowly added to feed, ratio is that feed per ton adds 400L to dilute bacterium Liquid.Being mixed uniformly to crush agglomerate.
(4) prepared feed is packed into fermentor, sealing is closed, guarantees that sealing is completely airtight.
(5) it was fermented according to season through (summer 5 days, spring and autumn 6 days, 7 days winters) 5~7 days, feed issues slightly sour-sweet Strong distiller's yeast fragrance mark ferments successfully.
It can Direct-fed after fermenting successfully.If the feeding dosage of fermentation one week, should all be rapid after taking every time Sealing.
In order to determine the feeding effect of growing and fattening pigs full price fermentative feedstuff of microbe, the present invention is provided with following scheme:
(1) control group:Unfermentable complete feed for fattening pigs.This group totally 24 pigs, are divided into 4 repetitions, each repetition 6 (contain following substance in feed:Corn 67%, dregs of beans 21%, wheat bran 8%, premix 4%).
(2) test group:Growing and fattening pigs YH bacterium (leavening of the invention) full price fermentative feedstuff of microbe.This group totally 24 pigs, It is divided into 4 repetitions, each repetition 6 (contains following substance in feed:Corn 67%, dregs of beans 21%, wheat bran 8%, premix 4%).
By feed formula material rate, corn, dregs of beans, wheat bran, premix are weighed, is placed in container;It will prepare Growing and fattening pigs YH bacterium full price fermentative feedstuff of microbe bacterium solution make an addition in container, be sealed fermentation.Fermentation time season through 5~ 7 days (summer 5 days, spring and autumn 6 days, 7 days winters).Growing and fattening pigs YH bacterium full price fermentative feedstuff of microbe is made, it is spare, it is educated Hog feeding.
(3) selection of growing and fattening pigs and feeding manner
The castrating boar of weight 55kg or so is selected, test group and control group respectively take 24, and totally 48, every group of 4 repetitions, 6 pigs of each repetition, divide colony house to raise, are freely eaten and drink water.
(4) pig house fattening hog slaughtering sampling
(1) test group respectively takes 6 pigs to be butchered with control group, and fasting for 24 hours before butchering started to butcher in second day early morning Sampling.
(2) longissimus dorsi muscle for acquiring pig, carries out related meat quality Indexs measure.
(5) the feeding effect evaluation of growing and fattening pigs YH bacterium full price fermentative feedstuff of microbe
Its growth performance and after slaughter its related meat quality index of sample detecting are counted in the growing-finishing pigs stage, mainly Testing index is as follows:
1, various nutriment detections in feed
(1) measurement that dry matter measures in feed
Weigh Feed Sample quality m1, dry-eye disease or half dry-eye disease are put into 105 ± 2 DEG C of baking ovens, at one atm Drying, until constant weight, claims Feed Sample quality m2
W=(m2/m1) × 100%
The mass fraction of dry matter, % in W-- feed
m1-- Feed Sample quality, g
m2-- Feed Sample after processing, g
(2) in feed coarse ash measurement
Weigh Feed Sample quality m1, Feed Sample is put into calcination in other 550 DEG C ± 5 DEG C of luxuriant good fortune furnace, until canescence, Claim the weight m of residue2
W=(m2/m1) × 100%
The mass fraction of coarse ash, % in W-- feed
m1-- Feed Sample quality, g
m2-- Feed Sample after processing, g
(3) in feed crude protein measurement
Under conditions of having catalyst, organic nitrogen is transformed into inorganic ammonium salt with concentrated sulfuric acid sample digestion, then in alkalinity Under the conditions of by ammonium salt convert ammonia, go out with steam distillation and be that excessive standard hydrochloric acid solution absorbs (V), then with standard mark hydrogen-oxygen Change sodium (Vo) titrate to terminal, calculate the nitrogen quantity in sample.
W (CP)=[(VC-VoCo) × 0.0140 × 6.25/m] × 100%
W (CP) -- the mass fraction of crude protein, % in feed
V-- titrates the volume of consumed standard hydrochloric acid solution when sample, mL
C-- titrates the concentration of consumed standard hydrochloric acid solution when sample, mol/mL
Vo-- the volume of consumed standard caustic soda solution, mL when titration sample
Co-- the concentration of consumed standard caustic soda solution, mol/mL when titration sample
The molal weight of 0.0140-- nitrogen, kg/mol
M-- sample mass, g
(4) in feed crude fat measurement
Weigh sample mass m.It selects low-boiling ether or petroleum ether to make solvent, weighs permanent extraction flask quality out m2, extract air-dry sample repeatedly in ether, fat is dissolved in ether and is collected in ether bottle, weighs permanent extraction flask out With the gross mass m of crude fat1, find out the content of ether extract, the i.e. content of crude fat.
W (EE)=[(m1-m2)/m] × 100%
W (EE) -- crude fat mass fraction in feed, %
m1-- the gross mass of permanent extraction flask and crude fat out, g
m2-- permanent extraction flask quality out, g
M-- air-dried sample quality, g
(5) total phosphorus yield in feed
By organic destruction in sample, make P elements separate out, in an acidic solution, handled with vanadium ammonium molybdate, generates The complex compound of yellow, carries out colorimetric estimation at wavelength 400nm, and result is total phosphorus content.
(6) calcium catalyst in feed
By sample organic destruction, calcium become the ion for being dissolved in water and reacted with hydrochloric acid generate calcium chloride, in the solution plus Enter ammonium oxalate solution, calcium is made to become calcium oxalate white precipitate, then dissolves calcium oxalate with sulfuric acid solution, then with potassium permanganate standard The free oxalate denominationby of titration solution titration can calculate style according to the dosage of potassium permanganate standard titration solution Calcium content.
W (Ca)=[(V-Vo)×N×40/2]/(W×V’/V1)×100/1000
V--0.01N Standard Potassium Permanganate Solution titration volume used, mL
Vo-- 0.01N Standard Potassium Permanganate Solution volume used in measurement blank, mL
The substance withdrawl syndrome of N-- Standard Potassium Permanganate Solution, mol/mL
W-- sample mass, g
V-- sample decomposed solution total volume, mL
V ' -- drawn sample decomposed solution volume, mL when measurement
The gram equivalent of 40/2-- calcium, g
(7) crude fibre measures in feed
Sample m is weighed with assay balance, is handled with sulfuric acid solution and n-octyl alcohol, boiling is quickly heated up to and maintains 30min.Immediately It filters (6.5 μm of the aperture Gooch collapsing Nei Pu nylon cloth).Residue distillation is washed to neutrality, drains, with 200mL, 0.313mol/ Residue is transferred to original completely and disappears by L sodium hydroxide (NaOH) solution to be boiled in molten device, and 30min is boiled.Immediately in 550 DEG C of constant weights Filter crucible spreads 0.2~0.3g of acid-washed asbestos filtering.First use 25mL, 0.255mol/L sulfuric acid (1/2H2SO4) solution washed once, then use Distilled water is sink to neutrality.It is primary with 15mL ethanol washing after draining.Filter crucible 130 DEG C of drying in oven 2h are put into after draining to take out It is put into drier cooling 30min, weighs m1.Calcination 1h in 550 DEG C of luxuriant good fortune furnaces is moved into again, and taking-up is cooled to 130 DEG C or so, puts Enter cooling 30min in drier, weighs m2
W (CF)=[(m1-m2)/m] × 100%
W (CF) -- coarse-fibred mass fraction in feed, 100%
m1-- filter crucible and sample residues gross mass after 130 DEG C of drying, g
m2-- filter crucible and sample residues gross mass after 550 DEG C of calcinations, g
The weighed sample mass of m--, g
2, growth performance
It weighs respectively before and after growing and fattening pigs feeding (original body mass of growing and fattening pigs and final weight), calculates average daily gain; Fattening pannage feed intake is counted, daily ingestion amount, feedstuff-meat ratio are calculated;Count feeding time.
3, meat
Meat quality determination is carried out after butchering to test pig, measuring method is referring to the matter performance measurement of NY/T 821-2004 pig muscle Technical specification.On-site measurement is carried out to indexs such as yellowish pink, marble grain, pH, drip loss, percentage of water loss, cooked meat percentages;Intramuscular fat Content, muscle fatty acid composition, the sampling of diameter of muscle fiber sign spot, take back experimental determination.
Specific assay method is as follows:
(1) yellowish pink measurement
1. sampling sites:Longissimus dorsi muscle at last thoracic vertebrae.
2. assessing terms:With the fresh meat sample butchered in rear 1~2h, range estimation and the mark that scores are carried out under normal illumination indoors Quasi- figure comparison evaluation.
3. evaluation criteria:By Color Score standard drawing, sample is evaluated using 5 points of systems yellowish pink:1 point-canescence;2 points- Blush;3 points-cerise;4 points-peony;5 points-kermesinus.If occur between two grades when scoring, It can be chosen as 0.5 point.Normal yellowish pink generally 3~4 points, then there are the suspicion of white muscles (PSE meat), 5 points or so of sample less than 2 points Then there is the possibility of black biltong (DFD meat).
(2) muscle marble grain
Evaluate the index of fat content and distribution situation in muscle.
1. sampling point:Thoracolumbar disk junction longissimus dorsi muscle.
2. assessing terms:Test pig is butchered in 1~2h, and 0~4 DEG C of refrigerator cold-storage carries out mesh for 24 hours, with NPPC color board made in U.S.A Assessment point.
3. standards of grading:Scoring is made using 5 points, allows to comment 0.5 point between two-stage.1 point (fatty trace);2 points (fat is micro- Amount);3 points (being measured in fat);4 points (fatty volume);5 points (fat is excessive).3~4 points of marble grain represent it is with good meat quality.
(3) muscle pH
Body development in pig postmortem muscle generates lactic acid by the glycolysis of anaerobic metabolism approach, with the accumulation flesh of lactic acid in muscle The pH of meat declines, the degree of pH decline to it is yellowish pink, be that waterpower, soluble protein concentration, shelf life length all have a significant effect, because But evaluate an important indicator of meat.
1. sampling point:Longissimus dorsi muscle at last thoracic vertebrae.
2. instrument:It is accurate to 0.01 PHS-25 type digital display acidometer (German R.Matthaus company PH-STAR), is measured Preceding pH meter must be corrected with pH4 and pH7 titer.
3. operating:45~60min measures chest waist junction longissimus dorsi muscle pH after bloodletting, as pH45.It is taken out for 24 hours after government official 0~4 DEG C of cooling eye muscle middle section is cut, and pH meter electrode insertion joint-cutting is measured pH24 (PHU).
(4) percentage of water loss measures
Longissimus dorsi muscle is taken at third and fourth thoracic vertebrae, is taken with sampler after test pig is killed in 1~2h using weight pressurization Radius and thickness are the small circle sheet of 1cm.Between meat sample is placed in two layers of gauze after weighing (W1, g), 18 layers are respectively padded above and below Filter paper is placed on the pressure device that induction amount is 0.019, pressure 35kg together together with filter paper, is taken out meat sample after the 5min that pressurizes and is claimed Weight (W2, g).Percentage of water loss is smaller, is that waterpower is higher.
Percentage of water loss W=[(W1-W2)/W1] × 100%.
(5) shearing force measures
Longissimus dorsi muscle waist paragraph header side sample is taken, is fitted into polybag, after 4 DEG C of refrigerators cure 48h, is stood at room temperature 1h.75~80 DEG C of water-baths are immersed, reaches 70 DEG C to meat sample central temperature and takes out meat sample, be cooled to 20 DEG C, by meat sample core Divide and accomplishes 1 × 1 × 2.5cm3Cutlet (it is consistent with longitudinal direction that muscle fibre moves towards direction), with C-LM type Meat Tenderness instrument survey cut Shear force.
(6) cooked meat percentage measures
After test pig is killed in 1~2h, greater psoas muscle middle section about 100g meat sample is taken, is weighed (W1, g), is placed on pot drawer and steams 30min After hang on shady place 15min, weigh (W2, g).Cooked meat percentage (%) ﹦ (W2/W1) × 100%.
(7) intramuscular fat content measurement (soxhlet extraction)
1. sampling point:After butchering, it is sent to laboratory as early as possible, takes longissimus dorsi muscle at the 6th~7 rib cage or the 11st~12 rib cage Marble grain scoring is carried out, intramuscular fat content measurement is then carried out.
2. pre-treatment:Air-drying sample is made for measuring meat sample over dry moisture content, 65 DEG C of a part in chopping, a part
3. instrument:Balance, semi-automatic soxhlet type instrument (2055SOXTEC Manual Extraction Unit)
Step:Air-dried 3~5g of meat sample filter paper is claimed to wrap up and number.Filter paper is wrapped in 103~105 DEG C of baking ovens and dries 2h. It moves into the extraction pipe of Soxhlet extractor and anhydrous ether is added, setting program (boiling 2h) recycles 103~105 after ether 10min is dried in DEG C baking oven, in drier after cooling 30min, weighing.
Fat (%)=[(W2-W1)/W] × 100%
In formula, W is air-drying sample weight (g), and W1 is fat collecting bottle weight (g) before extracting, and W2 is fat collecting bottle after extraction (g)。
(8) longissimus dorsi muscle Contents of Amino Acids
Claim 3~5g of meat sample, accurately to 0.0001g, then carry out degreasing, after degreaser drying, crushes.Weigh sample powder about 0.1g is fitted into ampere bottle, and the 6mol/L hydrochloric acid hydrolysis of 10mL is added, sets and seals after freezing 1~2min in liquid nitrogen, it is placed on 110 ± In 1 DEG C of thermostatic drying chamber, hydrolysis 22~for 24 hours.It is cooling, it mixes, all solution are transferred in cucurbit, are used in combination by open pipe 0.02mol/L HCl is rinsed ampere bottle 1~2 time, and cucurbit rotary evaporation vacuumizes, and water bath temperature is set as 65 DEG C, evaporation It is extremely dry.The 0.02mol/L HCl of 20mL is pipetted in cucurbit, pipettes 1mL solution in bottle after dry matter in 25mL after sufficiently dissolve Volumetric flask, with 0.02mol/L HCl constant volume.Upper machine analysis after taking 1mL to filter.0.200mL kilnitamin standard is drawn, pH is used 2.2 buffer is diluted to 5mL, this standard dilution concentration is 5.00nmol/50 μ L, the amino acidity scale as upper machine measurement Standard, with the full-automatic automatic amino acid analyzer of A300 with the amino acid content of external standard method Specimen Determination liquid.
4, test growing and fattening pigs blood parameters commission Shaanxi Province's construction company hospital measurement.
(6) evaluation result
Test is bought with full price basal feed from BeiJing DaBei farm Science Group Co., Ltd, is educated using of the invention The evaluation result of hog YH bacterium full price fermented feed and feeding method, fermentation and feeding effect is as follows:
Fermentation test daily ration and its trophic level comparison are raised in the fermentation of 2 growing and fattening pigs YH bacterium full price of table
Influence of the 3 YH bacterium full price fermented feed of table to growing-finishing pigs performance
Influence of the 4 YH bacterium full price fermented feed of table to growing and fattening pigs blood parameters
Influence of the 5 YH bacterium full price fermented feed of table to growing and fattening pigs routine meat
Influence of the 6 YH bacterium full price fermented feed of table to growing and fattening pigs nutritive composition in muscle
Influence of the 7 YH bacterium full price fermented feed of table to growing and fattening pigs Amino Acid Contents of Muscle
As shown in table 2, YH bacterium full price zymophyte powder fermentation test feed, daily ration and trophic level are just tested with conventional feed Comparison can be seen that after the fermentation of growing and fattening pigs YH bacterium full price, the thick nutrients such as crude protein, crude fat, coarse ash in former feed Matter is decomposed, and the constituent contents such as calcium phosphorus, which also have, largely to be changed.Show after fermentation that nutriment can be decomposed into small in feed Molecular substance plays the effect for promoting growth of animal, maintaining animal body health to be more advantageous to animal absorption.
As shown in table 3, after feeding YH bacterium full price fermented feed, when reaching close slaughter weight, the feeding of test group pig Period significantly shortens, and average daily gain and average daily gain are higher than control group, and feedstuff-meat ratio is lower than control group.Show that YH bacterium is complete Valence fermented feed can shorten the feeding cycle of growing and fattening pigs, reduce feedstuff-meat ratio, so as to improve the growth performance of growing and fattening pigs, section Save feeding cost.
As shown in table 4, after feeding YH bacterium full price fermented feed, the glutamic-pyruvic transaminase of test group pig blood is detected, millet straw turns Adnosine deaminase, alkaline phosphatase, calcium content, glucose, triglycerides and low density cholesterol are lower than control group.Serum glutamic pyruvic transminase It is the important indicator of liver and the reflection of heart organ function with glutamic-oxalacetic transaminease, if its index level is excessively high, illustrates corresponding Animal organ may centainly be damaged.In addition, the total protein of test group pig, globulin, albumin and Archon ratio are higher than control Group shows that YH bacterium full price fermented feed is conducive to fatten the health of pig body, improves its immune performance.
As shown in table 5, after feeding YH bacterium full price fermented feed, test group pig longissimus dorsi muscle muscle redness (a*) is significantly higher than Control group shows that YH bacterium full price fermented feed can improve yellowish pink.
As shown in table 6, crude protein and thick rouge after feeding YH bacterium full price fermented feed, in test group pig longissimus dorsi muscle muscle Fat is higher than control group, shows that YH bacterium full price fermented feed can be improved the content of growing and fattening pigs nutritive composition in muscle, is conducive to fatten The improvement of Meat.
As shown in table 7, after feeding YH bacterium full price fermented feed, test group pork Fresh ear field (aspartic acid, paddy ammonia Acid, alanine) it is significantly higher than control group, and essential amino acid (leucine, isoleucine) is also above control group, and variant Significant trend.Show that YH bacterium full price fermented feed can be improved the content of muscle Fresh ear field and essential amino acid, thus Muscle delicate flavour is improved, meat quality is improved.
Compared with existing growing and fattening pigs feeding program, the present invention is by lactobacillus fermenti, Bifidobacterium, clostridium butyricum and thermophilic Hot streptococcus is mixed in a certain ratio use, it is fermented after feed feeding growing and fattening pigs can significantly improve nutrition in feed after fermentation Substance decomposition is small-molecule substance to be more advantageous to animal absorption, plays and promotes growth of animal, maintains animal body health Effect.The feeding cycle of growing and fattening pigs can be shortened, reduce feedstuff-meat ratio, so as to improve the growth performance of growing and fattening pigs, save and raise Form this;The health for promoting fattening pig body, improves its immune performance;Improve yellowish pink;Improve growing and fattening pigs nutritive composition in muscle Content is conducive to the improvement of meat quality of fattening pigs;The content for improving muscle Fresh ear field and essential amino acid, to improve muscle Delicate flavour improves meat quality.
It should be understood that it will be understood by those skilled in the art that plan content can be improved according to the above description and Transformation, and all these modifications and variations should all belong to the protection domain of appended claims of the present invention, but these improve and become Change so that the effect under group of formula level is unpredictable compared with the prior art.

Claims (5)

1. a kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation leavening, which is characterized in that the leavening includes hair Kefir milk bacillus, Bifidobacterium, clostridium butyricum and streptococcus thermophilus.
2. growing and fattening pigs full price fermentative feedstuff of microbe preparation leavening as described in claim 1, which is characterized in that in leavening Dosage relation is between each component:1kg powder starter is made of the component of following mass percent:Lactobacillus fermenti 63.18%, fourth Sour clostridium 23.70%, Bifidobacterium 10.56%, streptococcus thermophilus 2.56%.
3. a kind of growing and fattening pigs full price fermentative feedstuff of microbe preparation method, which is characterized in that method includes:
Fermentation prepares fermented feed under closed environment after bacterium solution and basal feed are mixed, and the bacterium solution is by claims 1 or 2 The leavening and water are formulated;The basal feed is formulated by corn, dregs of beans, wheat bran and premix.
4. growing and fattening pigs full price fermentative feedstuff of microbe preparation method as claimed in claim 3, which is characterized in that raise on the basis Material is that 67% corn, 21% dregs of beans, 8% wheat bran and 4% premix are formulated by mass percent;
The bacterium solution adds the ratio of 1kg bacterium powder to be formulated by every 40L water;
The dosage relation of the basal feed and bacterium solution is:Basal feed per ton adds 400L to dilute bacterium solution.
5. growing and fattening pigs full price fermentative feedstuff of microbe preparation method as claimed in claim 4, which is characterized in that fermentation temperature 30 It~35 DEG C, fermented through 5~7 days, obtains fermented feed.
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