CN108813093A - A kind of method that high-efficient cleaning clean prepares rich peptide rapeseed dregs - Google Patents
A kind of method that high-efficient cleaning clean prepares rich peptide rapeseed dregs Download PDFInfo
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- CN108813093A CN108813093A CN201810494827.0A CN201810494827A CN108813093A CN 108813093 A CN108813093 A CN 108813093A CN 201810494827 A CN201810494827 A CN 201810494827A CN 108813093 A CN108813093 A CN 108813093A
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Abstract
The invention discloses a kind of methods that high-efficient cleaning clean prepares rich peptide rapeseed dregs, are related to technical field of biological fermentation.The method that high-efficient cleaning clean provided by the invention prepares rich peptide rapeseed dregs includes：Wheat bran is mixed into rapeseed dregs, is carried out high-temperature maturing sterilizing after wetting with water, is obtained sterile curing material；Access strain carries out aerobic fermentation after curing material cooling, obtains fermentation materials；Strain is single strain；Fermentation materials are digested, enzymatic hydrolysis material is obtained；Enzymatic hydrolysis enzyme used is the enzyme of generation during strain fermentation；It is obtained by drying material will to be digested.This method carries out single strain fermentation after curing rapeseed dregs high-temperature sterilization, be conducive to target strain and form dominant strain, inhibit the breeding of the raised growth of miscellaneous bacteria and harmful bacteria, improve the efficiency and spatter property of fermentation process, improve fermentation efficiency, after fermentation, the enzyme for making full use of fermentation process bacterial strain to generate digests fermentation rapeseed dregs, improves trophism, safety and the palatability of fermentation rapeseed dregs.
Description
Technical field
The present invention relates to technical field of biological fermentation, especially a kind of method of the rich peptide rapeseed dregs of high-efficient cleaning clean preparation.
Background technique
Rapeseed dregs is by-product of the rapeseed after extracting grease, and wherein crude protein content is 30%-40%, is contained simultaneously
Minerals and vitamins abundant are extremely balanced containing amino acid classes, and cheap, are a kind of great exploitation potentials
Vegetable protein resource.But since rapeseed dregs proteopepsis absorptivity is lower, color, smell are more special, and containing there are many anti-
Trophic factors and toxin, such as tannic acid, phytic acid, sulphur glycosides not only influence the palatability as feed, and reduce animal pair
The digestion and absorption of albumen, especially toxin sulphur glycosides may cause Thyroid Gland Swell, limitation growth of animal, even after animal edible
It is poisoned to death.Therefore large-scale use of the rapeseed dregs in feedstuff industry is seriously limited.
In the prior art, the following steps are generally included to the utilization method of rapeseed dregs：Inoculation is appropriate in varing proportions
The hybrid bacterial strain of bacterial strain cooperation（Mainly it is made of lactic acid bacteria, bacillus, yeast）It is mixed with a certain proportion of water and rapeseed dregs
It closes, by uniformly mixed material without any processing, controls suitable temperature and directly carry out solid state fermentation after a certain period of time, then
Drying obtains fermentation rapeseed dregs, then adds auxiliary material appropriate and feed is made.The major defect of this method is：
（1）It is raw material when rapeseed dregs is fermented, rapeseed protein can not be moderately denaturalized, it is difficult to be inhibited contained in raw material rapeseed dregs
The influence to fermentation of miscellaneous bacteria and harmful bacteria, clean that degree is low, safety of fermentation rapeseed dregs so as to cause zymotechnique
The activity and level that property cannot get various enzymes after effective guarantee, fermentation are relatively low.
（2）During Rapeseed Meal by Aspergillus Fermentation, mostly using two kinds and two or more bacterial strain mixed fungus fermentations and inoculum concentration it is lower, deposit
Bacterial strain seed preparation process is many and diverse, aimed strain is difficult to form dominant bacteria, thus it is difficult to inhibit a large amount of of miscellaneous bacteria and harmful bacteria
The disadvantages of growth and breeding, longer fermentation period.
（3）It is dried immediately after Rapeseed Meal by Aspergillus Fermentation, causes the various enzymes that bacterial strain generates in fermentation process, especially
Protease, which fails to play one's part to the full, to be just inactivated, and vegetable seed cake protein is without appropriate denaturation in addition, so that fermentation rapeseed dregs
Small peptide content is generally lower.
Summary of the invention
Goal of the invention of the invention is：In view of the above problems, a kind of rich peptide dish of high-efficient cleaning clean preparation is provided
The method of the seed dregs of rice, this method carry out single strain fermentation after curing rapeseed dregs high-temperature sterilization, are conducive to inhibit miscellaneous bacteria and harmful bacteria
Raised growth breeding, improve the spatter property of fermentation process, single strain fermentation makes aimed strain form dominant strain, improves fermentation
Efficiency, and after fermentation, make full use of the various high active enzymes that bacterial strain generates in fermentation process to carry out fermentation rapeseed dregs
Enzymatic hydrolysis improves trophism, safety and the palatability of fermentation rapeseed dregs.
The technical solution adopted by the present invention is as follows：
A kind of method that high-efficient cleaning clean prepares rich peptide rapeseed dregs comprising following steps：
（1）Wheat bran is mixed into rapeseed dregs, is carried out high-temperature maturing sterilizing after wetting with water, is obtained sterile curing material；
（2）Curing material accesses strain after cooling and carries out aerobic fermentation, obtains fermentation materials；The strain is single strain；
（3）Fermentation materials are digested, enzymatic hydrolysis material is obtained；Enzymatic hydrolysis enzyme used is the enzyme of generation during strain fermentation；
（4）It is obtained by drying material will to be digested.
By adopting the above-described technical solution, rapeseed dregs is killed wherein original through high-temperature maturing, one side high temperature before fermenting
The miscellaneous bacteria contained and harmful bacteria, improve fermentation process clean and safety, on the other hand, protein under high temperature action fit
Degree denaturation, improving it can enzymatic hydrolyzation.Fermentation process is fermented using single strain, simplifies the preparation process of fermentation seed, and is conducive to
Target strain forms dominant strain, to inhibit the growth and breeding of miscellaneous bacteria, is conducive to ferment control, improves fermentation efficiency, shortens
Fermentation period, while single strain fermentation is conducive to a large amount of generations of high active enzyme there is no the effect of antagonism mutual between bacterial strain.
After fermentation, fermentation materials are digested using the various high active enzymes generated in fermentation process, on the one hand by macromolecule protein
Further enzymatic hydrolysis is small peptide, improves small peptide content, and the enzyme on the other hand making full use of fermentation process to generate does not add exogenous
Enzyme, save the cost.In addition, the toxin and anti-nutritional factors in rapeseed dregs can also be digested in enzymolysis process, fermentation dish is improved
The safety and palatability of the seed dregs of rice.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, rapeseed dregs account for the 70- of dry material total weight
90%, wheat bran accounts for the 10-30% of dry material total weight.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, the temperature of the water of addition are 40-60 DEG C, are added
The water content of material is the 30-60% of total weight after water, mixes thoroughly after adding water and standing 20-50min makes material fully absorb moisture,
Then the curing sterilizing 15-30min at 110-135 DEG C.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, step（2）In, the strain is black song
It is mould.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, the strain are prepared via a method which：
Wheat bran and water are uniformly mixed, wheat bran water content 30%-60%, 110 DEG C of -135 DEG C of sterilizing 10-30min is made to be cooled to 30-40
Inoculating strain after DEG C, then controlling temperature of charge is 30-35 DEG C, and culture obtains strain in 2-5 days.
By adopting the above-described technical solution, the strain being prepared is high to the adaptability of fermentation substrate, fermentation activity is high,
Have and utilizes raising fermentation efficiency.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, the access amount of the strain are the curing
The 3-7% of material.
It is further object bacteria by adopting the above-described technical solution, compared with prior art, substantially increasing inoculum concentration
Kind becomes dominant bacteria and provides condition.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, the temperature of aerobic fermentation maintain 20-40
DEG C, ferment 30-48h.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, the step（3）In, before enzymatic hydrolysis, Xiang Fa
The water content for adding water or microbial fermentation solution to fermentation materials in ferment material is 50-60%, and the microbial fermentation solution is selected from lactic acid
At least one of fermented liquid, yeast fermentation broth, fermentation of bacillus liquid or clostridium butyricum fermentation liquid.
By adopting the above-described technical solution, the water content of material increases its water content, favorably 35% or so after fermentation
It in enzymolysis process efficiently carries out, it is various rich by what is generated in biological fermentation process simultaneously to improve water content using bio-fermented liquid
The material after enzymatic hydrolysis is added in rich metabolite, further increases its trophism.
It should be noted that streptococcus acidi lactici fermented solution, yeast fermentation broth, fermentation of bacillus liquid or butyric acid that the present invention uses
Clostridial fermentation liquid is all made of this field volume conventional technical means and obtains, and it will not go into details herein.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, enzymolysis process is anaerobic processes, control temperature
Degree is 30-60 DEG C, and enzymolysis time is 24-48 hours.
The method that a kind of high-efficient cleaning clean of the invention prepares rich peptide rapeseed dregs, step（4）In, material is digested in 65-70
DEG C water content is dried to less than 10%.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
（1）High-temperature maturing sterilization treatment is carried out to raw material rapeseed dregs before fermentation, not only killed miscellaneous bacteria in raw material rapeseed dregs and
Harmful bacteria, and rapeseed protein is made to have obtained appropriate denaturation under the action of heating power, to realize the cleaning of zymotechnique
Change and make the safety of fermentation rapeseed dregs and the degradable rate of rapeseed protein to be greatly improved.
（2）It is fermented using single strain, while using large scale inoculation fermentation bacterial strain, be conducive to the rapid shape of aimed strain
At dominant strain, inhibit the growth and breeding of miscellaneous bacteria and harmful bacteria, guarantees that fermentation starting is rapid, fermenting speed is fast, so as to shorten hair
The ferment period, while single strain fermentation is conducive to a large amount of generations of high active enzyme there is no the effect of antagonism mutual between bacterial strain.
（3）It is especially provided with enzymolysis process process, in enzymolysis process, the enzyme generated using fermentation process is to without sufficiently sending out
The substance of ferment is digested, and the various high activities of one side fermentation process generation, high-level enzyme are fully utilized, on the other hand
Rapeseed protein without sufficiently fermenting is digested completely, increases substantially the content of fermentation rapeseed dregs Small Peptides, in addition, generating
Enzyme by rapeseed dregs toxin and anti-nutritional factors digest, improve fermentation rapeseed dregs safety and palatability, to mention
Its high practical value.
Specific embodiment
All features disclosed in this specification or disclosed all methods or in the process the step of, in addition to mutually exclusive
Feature and/or step other than, can combine in any way.
This specification（Including any accessory claim, abstract）Disclosed in any feature, unless specifically stated,
It is replaced by other equivalent or with similar purpose alternative features.That is, unless specifically stated, each feature is a series of
An example in equivalent or similar characteristics.
Embodiment 1
The present embodiment provides a kind of methods that high-efficient cleaning clean prepares rich peptide rapeseed dregs, and the method achieve the efficient hairs cleaned
Ferment, and by enzymatic hydrolysis and fermentation material make improve product safety, trophism and palatability.This method comprises the following steps：
Step 1：Rapeseed dregs and wheat bran are taken respectively, is stirred evenly after adding water, so that rapeseed dregs and wheat bran is distinguished complete wetting, then
Material after wetting is subjected to high-temperature sterilization curing, obtains curing material.
Step 2：Curing material is cooled to room temperature, round is transferred to, and access single strain carries out aerobic hair thereto
Ferment obtains fermentation materials.
Step 3：Fermentation materials are digested, enzymatic hydrolysis material is obtained, enzymolysis process directly utilizes during aerobic fermentation
Generated enzyme does not add any exogenous enzymes.
Step 4：Will enzymatic hydrolysis drying materials to get to the rich peptide rapeseed dregs cleaned.
Embodiment 2
The present embodiment provides a kind of methods that high-efficient cleaning clean prepares rich peptide rapeseed dregs, and the method achieve the efficient hairs cleaned
Ferment, and by enzymatic hydrolysis and fermentation material make improve product safety, trophism and palatability.This method comprises the following steps：
Step 1：The wheat bran 10kg for taking rapeseed dregs 90kg respectively, stirs evenly after adding water 50kg, stand 20min, make rapeseed dregs and
Wheat bran distinguishes complete wetting, and then sterilize the material after wetting curing 30min at 110 DEG C, obtains curing material.
Step 2：Curing material is cooled to room temperature, round is transferred to, it is aerobic to access Aspergillus niger strain progress thereto
Fermentation, obtains fermentation materials.
Step 3：Add water into fermentation materials, makes its water content 50%, allow fermentation materials to be digested, obtain zymolyte
Material, enzymolysis process directly utilize generated enzyme during aerobic fermentation, do not add any exogenous enzymes.
Step 4：Will enzymatic hydrolysis drying materials to get to the rich peptide rapeseed dregs cleaned.
Embodiment 3
The present embodiment provides a kind of methods that high-efficient cleaning clean prepares rich peptide rapeseed dregs, and the method achieve the efficient hairs cleaned
Ferment, and by enzymatic hydrolysis and fermentation material make improve product safety, trophism and palatability.This method comprises the following steps：
Step 1：The wheat bran 27kg for taking rapeseed dregs 81kg respectively is stirred evenly after adding 40 DEG C of warm water 72kg, is stood 50min, is made dish
The seed dregs of rice and wheat bran distinguish complete wetting, and then sterilize the material after wetting curing 15min at 135 DEG C, obtain curing material.
Step 2：Curing material is cooled to room temperature, round is transferred to, guarantees that it, with a thickness of 10-50cm, connects thereto
Enter 12.6kg aspergillus niger strain, stir evenly, maintaining temperature is 20 DEG C, and aerobic fermentation 48h obtains fermentation materials.
Step 3：Streptococcus acidi lactici fermented solution is added into fermentation materials, makes its water content 55%, then fermentation materials is allowed to exist
48h is digested at 30 DEG C, obtains enzymatic hydrolysis material, enzymolysis process directly utilizes generated enzyme during aerobic fermentation, do not add and appoint
What exogenous enzymes, and enzymolysis process maintains anaerobic conditions within.
Step 4：Will enzymatic hydrolysis drying materials to get to the rich peptide rapeseed dregs cleaned.
Embodiment 4
The present embodiment provides a kind of methods that high-efficient cleaning clean prepares rich peptide rapeseed dregs, and the method achieve the efficient hairs cleaned
Ferment, and by enzymatic hydrolysis and fermentation material make improve product safety, trophism and palatability.This method comprises the following steps：
Step 1：The wheat bran 36kg for taking rapeseed dregs 84kg respectively is stirred evenly after adding 50 DEG C of warm water 180kg, is stood 30min, is made
Rapeseed dregs and wheat bran distinguish complete wetting, and then sterilize the material after wetting curing 20min at 125 DEG C, obtain curing object
Material.
Step 2：Curing material is cooled to room temperature, round is transferred to, guarantees that it, with a thickness of 10-50cm, connects thereto
Enter 9kg aspergillus niger strain, stir evenly, maintaining temperature is 40 DEG C, and aerobic fermentation 30h obtains fermentation materials.
Step 3：The mixture of saccharomycetes to make fermentation liquid and fermentation of bacillus liquid is added into fermentation materials, keeps its aqueous
Amount is 55%, and fermentation materials is then allowed to digest at 60 DEG C for 24 hours, obtains enzymatic hydrolysis material, and enzymolysis process directly utilizes aerobic fermentation mistake
Generated enzyme in journey, does not add any exogenous enzymes, and enzymolysis process maintains anaerobic conditions within.
Step 4：Enzymatic hydrolysis material at 65-70 DEG C is dried to water content less than 10% to get to the rich peptide vegetable seed cleaned
The dregs of rice.
Embodiment 5
The present embodiment provides a kind of methods that high-efficient cleaning clean prepares rich peptide rapeseed dregs, and the method achieve the efficient hairs cleaned
Ferment, and by enzymatic hydrolysis and fermentation material make improve product safety, trophism and palatability.This method comprises the following steps：
Step 1：The wheat bran 20kg for taking rapeseed dregs 80kg respectively is stirred evenly after adding 60 DEG C of warm water 100kg, is stood 45min, is made
Rapeseed dregs and wheat bran distinguish complete wetting, and then sterilize the material after wetting curing 25min at 120 DEG C, obtain curing object
Material.
Step 2：Curing material is cooled to room temperature, round is transferred to, guarantees that it, with a thickness of 10-50cm, connects thereto
Enter 10kg aspergillus niger strain, stir evenly, maintaining temperature is 25 DEG C, and aerobic fermentation 36h obtains fermentation materials.
Step 3：The mixture of saccharomycetes to make fermentation liquid, bacillus and clostridium butyricum fermentation liquid is added into fermentation materials,
Make its water content 60%, fermentation materials is then allowed to digest 36h at 45 DEG C, obtain enzymatic hydrolysis material, enzymolysis process is directly made good use of
Enzyme caused by during aerobe fermentation, does not add any exogenous enzymes, and enzymolysis process maintains anaerobic conditions within.
Step 4：Enzymatic hydrolysis material at 65-70 DEG C is dried to water content less than 10% to get to the rich peptide vegetable seed cleaned
The dregs of rice.
Embodiment 6
The present embodiment provides a kind of preparation method of fermentation seed, this method is used in fermentation of Aspergillus niger strain in embodiment 2-5
Preparation, specifically comprising following steps：
Take wheat bran, be uniformly mixed after warm water is added, make wheat bran water content 30%-60%, after wheat bran sufficiently soaks, 110 DEG C-
Sterilize 10-30min under the conditions of 135 DEG C, is cooled to 30-40 DEG C, then inoculated aspergillus niger strain thereto, controlled at 30-
35 DEG C, culture obtains strain in 2-5 days.
The invention is not limited to specific embodiments above-mentioned.The present invention, which expands to, any in the present specification to be disclosed
New feature or any new combination, and disclose any new method or process the step of or any new combination.
Claims (10)
1. a kind of method that high-efficient cleaning clean prepares rich peptide rapeseed dregs, which is characterized in that it includes the following steps：
（1）Wheat bran is mixed into rapeseed dregs, is carried out high-temperature maturing sterilizing after wetting with water, is obtained sterile curing material；
（2）Curing material accesses strain after cooling and carries out aerobic fermentation, obtains fermentation materials；The strain is single strain；
（3）Fermentation materials are digested, enzymatic hydrolysis material is obtained；Enzymatic hydrolysis enzyme used is the enzyme of generation during strain fermentation；
（4）It is obtained by drying material will to be digested.
2. the method that high-efficient cleaning clean according to claim 1 prepares rich peptide rapeseed dregs, which is characterized in that rapeseed dregs accounts for dry
The 70-90% of material total weight, wheat bran account for the 10-30% of dry material total weight.
3. the method that high-efficient cleaning clean according to claim 2 prepares rich peptide rapeseed dregs, which is characterized in that the water of addition
Temperature is 40-60 DEG C, add material after water water content be total weight 30-60%, mixing thoroughly after adding water and standing 20-50min makes object
Material fully absorbs moisture, then the curing sterilizing 15-30min at 110-135 DEG C.
4. the method that high-efficient cleaning clean according to claim 1 prepares rich peptide rapeseed dregs, which is characterized in that step（2）In,
The strain is aspergillus niger.
5. the method that high-efficient cleaning clean according to claim 4 prepares rich peptide rapeseed dregs, which is characterized in that the strain is logical
Cross following method preparation：Wheat bran and water are uniformly mixed, wheat bran water content 30%-60%, 110 DEG C of -135 DEG C of sterilizing 10- are made
30min, inoculating strain after being cooled to 30-40 DEG C, then controlling temperature of charge is 30-35 DEG C, and culture obtains strain in 2-5 days.
6. the method that high-efficient cleaning clean according to claim 4 prepares rich peptide rapeseed dregs, which is characterized in that the strain
Access amount is the 3-7% of the curing material.
7. the method that high-efficient cleaning clean according to claim 4 prepares rich peptide rapeseed dregs, which is characterized in that aerobic fermentation
Temperature maintains 20-40 DEG C, and ferment 30-48h.
8. the method that high-efficient cleaning clean according to claim 1 prepares rich peptide rapeseed dregs, which is characterized in that step（3）In,
Before enzymatic hydrolysis, the water content for adding water or microbial fermentation solution to fermentation materials into fermentation materials is 50-60%, the microorganism hair
Zymotic fluid is selected from least one of streptococcus acidi lactici fermented solution, yeast fermentation broth, fermentation of bacillus liquid or clostridium butyricum fermentation liquid.
9. the method that high-efficient cleaning clean according to claim 8 prepares rich peptide rapeseed dregs, which is characterized in that enzymolysis process is
Anaerobic processes, controlled at 30-60 DEG C, enzymolysis time is 24-48 hours.
10. the method that high-efficient cleaning clean according to claim 1 prepares rich peptide rapeseed dregs, which is characterized in that step（4）
In, enzymatic hydrolysis material is dried to water content at 65-70 DEG C less than 10%.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720853A (en) * | 2009-10-19 | 2010-06-09 | 李军训 | Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics |
-
2018
- 2018-05-22 CN CN201810494827.0A patent/CN108813093A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720853A (en) * | 2009-10-19 | 2010-06-09 | 李军训 | Technology for fermenting and decomposing protein feed with protease in two steps by using probiotics |
Non-Patent Citations (1)
| Title |
|---|
| 孙林 等: "菌种和发酵条件对菜籽粕中植酸和单宁的影响", 《养猪》 * |
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