CN106689668A - Preparation method of microbial fermented feed - Google Patents
Preparation method of microbial fermented feed Download PDFInfo
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- CN106689668A CN106689668A CN201710005844.9A CN201710005844A CN106689668A CN 106689668 A CN106689668 A CN 106689668A CN 201710005844 A CN201710005844 A CN 201710005844A CN 106689668 A CN106689668 A CN 106689668A
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- watermelon peel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention belongs to the technical field of feed processing, and in particular relates to a preparation method of microbial fermented feed. The method comprises the steps of: washing watermelon peel clean, and smashing the watermelon peel into a watermelon peel thick liquid by a fruit and vegetable beater, evenly mixing the watermelon peel thick liquid and a corn protein powder, adding acid protease, conducting enzymolysis to obtain a mixed enzymatic hydrolysate, evenly mixing the mixed enzymatic hydrolysate, wheat bran and rice bran, and then conducting sterilization, cooling, Lactobacillus bulgaricus fermentation, Candida utilis fermentation, cold drying, smashing, prilling, and packing to obtain the finished product. In addition to the functions of growth promotion, easiness for digestive absorption, immunity enhancement, etc. shared by common fermented feed, the microbial fermented feed also has a strong anti-stress effect.
Description
Technical field
The invention belongs to feed processing technology field, and in particular to a kind of preparation method of fermentative feedstuff of microbe.
Background technology
Watermelon peel is the accessory substance of generation in watermelon deep processing production process, and watermelon peel contains carbohydrate, thick egg
In vain, the nutriment such as crude fat, crude fibre, chlorophyll, also contains carrotene, thiamine, riboflavin, niacin, ascorbic acid
Deng the mineral salt such as vitamin and calcium, phosphorus, iron, in addition, also containing enzymes such as protease, glutamate decarboxylases, watermelon peel can be made
It is a kind of nutritious, good palatability animal and fowl fodder.Most of domestic watermelon deep processing enterprise all loses watermelon peel at present
Abandon without only minority is used to feed livestock and poultry, and this not only causes very big waste, and discarded watermelon peel also pollutes ring
Border, therefore, the comprehensive processing and utilization of watermelon peel is a problem anxious to be resolved, because watermelon peel water content is high, so easily
Rot smelly, pollution environment, wants raw material to make fermented feed based on watermelon peel, can both efficiently solve watermelon processing enterprise
The problem of watermelon peel treatment, can expand feed resource again, alleviate the problem of China's feedstuff shortage.
The content of the invention
It is an object of the invention to provide a kind of preparation method of the fermentative feedstuff of microbe for wanting raw material based on watermelon peel
The object of the present invention is achieved like this：
Watermelon peel is cleaned and watermelon latex is broken into fruit and vegetable pulping machine, by watermelon latex and corn protein powder according to mass ratio
10:1 ratio is well mixed to obtain mixed serum, and mixed serum pH is adjusted into 4.8-5.2, and 8- is added by every milliliter of mixed serum
The ratio of 12 units adds acid protease, and mixed enzymolysis liquid is digested 4-6 hours to obtain under the conditions of 37-45 DEG C.
By mixed enzymolysis liquid, wheat bran, rice bran according to mass ratio 7:2:1 ratio mixing, in 121 DEG C of conditions after stirring
Lower sterilizing 20-30 minutes, is cooled to 37-42 DEG C, and lactobacillus bulgaricus freeze-dried powder is accessed according to 0.01% inoculum concentration, mixes thoroughly
Afterwards anaerobic fermentation 12-18 hours under the conditions of 37-42 DEG C, the inoculum concentration according still further to 10% accesses candida utili seed liquor, mixes
It is taped against on tray after even, is fermented 50-60 hours in relative humidity 85-90%, the growth cabinet of 26-30 DEG C of temperature, then through low
Temperature is dried, crushed, granulating, packaging gets product.
Present invention additionally comprises following feature：The preparation method of described candida utili seed liquor is：Take 5 ° of B é malt
Juice, sterilizes 15-20 minutes under the conditions of 121 DEG C, is cooled to 25-30 DEG C, candida utili is inoculated with, in 25-30 DEG C, 150-
Shaking table culture 16-20 hours under conditions of 200rpm.
The fermentative feedstuff of microbe made of the inventive method, nutritious, good palatability, not only mycetome albumen, small
The nutriments such as peptide, multivitamin, microbial enzyme, also rich in GABA the effects such as composition, so micro- life of the invention
Thing fermented feed, in addition to the growth promotion with common fermentation feed, absorption easy to digest, strengthen immunity etc. are acted on, also has
The effect of very strong resisting stress.
Specific embodiment
The bacterial strain candida utili CICC32232 that the present invention is used is purchased in the management of Chinese industrial Microbiological Culture Collection
Center.
5 ° of B é brewer's worts are taken, is sterilized 20 minutes under the conditions of 121 DEG C, be cooled to 28 DEG C, candida utili is inoculated with, 28
DEG C, shaking table culture obtains final product candida utili seed liquor in 18 hours under conditions of 180rpm.
Watermelon peel is cleaned and watermelon latex is broken into fruit and vegetable pulping machine, by watermelon latex and corn protein powder according to quality
Than 10:1 ratio is well mixed to obtain mixed serum, and mixed serum pH is adjusted into 5.0, and 10 are added by every milliliter of mixed serum
The ratio of unit adds acid protease, and mixed enzymolysis liquid is digested 5 hours to obtain under the conditions of 40 DEG C.
By mixed enzymolysis liquid, wheat bran, rice bran according to mass ratio 7:2:1 ratio mixing, in 121 DEG C of conditions after stirring
Lower sterilizing 25 minutes, is cooled to 40 DEG C, lactobacillus bulgaricus freeze-dried powder is accessed according to 0.01% inoculum concentration, 40 after mixing thoroughly
Anaerobic fermentation 15 hours under the conditions of DEG C, the inoculum concentration according still further to 10% accesses candida utili seed liquor, and tray is taped against after mixing thoroughly
On, fermented 55 hours in relative humidity 85%, the growth cabinet of 28 DEG C of temperature, then through low temperature drying, crushing, granulation, packaging
Get product.
Claims (1)
1. a kind of preparation method of fermentative feedstuff of microbe, it is characterized in that being prepared from by following process：
(1) 5 ° of B é brewer's worts are taken, are sterilized 15-20 minutes under the conditions of 121 DEG C, be cooled to 25-30 DEG C, be inoculated with candida utili,
Obtain final product candida utili seed liquor within shaking table culture 16-20 hours under conditions of 25-30 DEG C, 150-200rpm；
(2) watermelon peel is cleaned and break into watermelon latex with fruit and vegetable pulping machine, by watermelon latex and corn protein powder according to mass ratio
10:1 ratio is well mixed to obtain mixed serum, and mixed serum pH is adjusted into 4.8-5.2, and 8- is added by every milliliter of mixed serum
The ratio of 12 units adds acid protease, and mixed enzymolysis liquid is digested 4-6 hours to obtain under the conditions of 37-45 DEG C；
(3) by mixed enzymolysis liquid, wheat bran, rice bran according to mass ratio 7:2:1 ratio mixing, after stirring under the conditions of 121 DEG C
Sterilizing 20-30 minutes, is cooled to 37-42 DEG C, lactobacillus bulgaricus freeze-dried powder is accessed according to 0.01% inoculum concentration, after mixing thoroughly
Anaerobic fermentation 12-18 hours under the conditions of 37-42 DEG C, the inoculum concentration according still further to 10% accesses candida utili seed liquor, mixes thoroughly
After be taped against on tray, fermented 50-60 hours in relative humidity 85-90%, the growth cabinet of 26-30 DEG C of temperature, then through low temperature
Dry, crush, granulating, packaging gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710005844.9A CN106689668A (en) | 2017-01-05 | 2017-01-05 | Preparation method of microbial fermented feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710005844.9A CN106689668A (en) | 2017-01-05 | 2017-01-05 | Preparation method of microbial fermented feed |
Publications (1)
| Publication Number | Publication Date |
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| CN106689668A true CN106689668A (en) | 2017-05-24 |
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ID=58905836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201710005844.9A Withdrawn CN106689668A (en) | 2017-01-05 | 2017-01-05 | Preparation method of microbial fermented feed |
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| CN (1) | CN106689668A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107212390A (en) * | 2017-06-16 | 2017-09-29 | 哈尔滨伟平科技开发有限公司 | A kind of health ferment and preparation method thereof |
| CN108065048A (en) * | 2017-12-22 | 2018-05-25 | 湖南九鼎动物营养研究院有限公司 | A kind of using bean dregs and rice bran is the preparation method of the fermented feed of primary raw material |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640839A (en) * | 2012-04-27 | 2012-08-22 | 赤峰市农牧科学研究院 | Efficient chicken manure fermenting agent and preparation method and application thereof |
| CN104171408A (en) * | 2014-08-02 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Method for preparing laying-hen feed additive by using potato residues |
| CN105851481A (en) * | 2016-05-29 | 2016-08-17 | 哈尔滨伟平科技开发有限公司 | Preparation method of milk cow feed additive |
| CN105995079A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of pig feed additive |
| CN105994942A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of feed additive for laying ducks |
-
2017
- 2017-01-05 CN CN201710005844.9A patent/CN106689668A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102640839A (en) * | 2012-04-27 | 2012-08-22 | 赤峰市农牧科学研究院 | Efficient chicken manure fermenting agent and preparation method and application thereof |
| CN104171408A (en) * | 2014-08-02 | 2014-12-03 | 哈尔滨伟平科技开发有限公司 | Method for preparing laying-hen feed additive by using potato residues |
| CN105851481A (en) * | 2016-05-29 | 2016-08-17 | 哈尔滨伟平科技开发有限公司 | Preparation method of milk cow feed additive |
| CN105995079A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of pig feed additive |
| CN105994942A (en) * | 2016-05-29 | 2016-10-12 | 哈尔滨伟平科技开发有限公司 | Preparation method of feed additive for laying ducks |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107212390A (en) * | 2017-06-16 | 2017-09-29 | 哈尔滨伟平科技开发有限公司 | A kind of health ferment and preparation method thereof |
| CN108065048A (en) * | 2017-12-22 | 2018-05-25 | 湖南九鼎动物营养研究院有限公司 | A kind of using bean dregs and rice bran is the preparation method of the fermented feed of primary raw material |
| CN108065048B (en) * | 2017-12-22 | 2021-06-11 | 湖南九鼎动物营养研究院有限公司 | Preparation method of fermented feed using bean dregs and rice bran as main raw materials |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20170524 |
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| WW01 | Invention patent application withdrawn after publication |