实施例2:3种长链非编码RNAs作为结直肠癌血清标志物的ROC分析
分别按照结直肠癌患者与正常人血清中实时定量PCR的结果,根据-△△ ct求值并做起相应的ROC曲线,观察曲线下面积及95%置信区间的范围来确定其灵敏性和特异性;ROC曲线下的面积值在1.0和0.5之间,在 AUC>0.5的情况下,AUC越接近于1,说明诊断效果越好。AUC在0.5~0.7 时有较低准确性;AUC在0.7~0.9时有一定准确性;AUC在0.9以上时有较高准确性;AUC=0.5时,说明诊断方法完全不起作用,无诊断价值;AUC<0.5 不符合真实情况,在实际中极少出现。
如图2所示,CCAT2、LINC00511和LINC01133的AUC值分别为 0.603、0.672、0.713。本发明还分析了目前临床上常用来作为结直肠癌诊断的血清标记物CA19-9与CEA的曲线下面积,AUC值分别为0.703(95%可信区间IC:0.641-0.765)与0.829(95%可信区间IC:0.781-0.877)。本发明联合3 种长链非编码RNAs与CA19-9、CEA绘制ROC曲线,敏感性与特异性分别为77.2%与92.9%,约登指数为0.701,并且AUC值达到了0.911(95%可信区间IC:0.87-0.951),见图3。研究结果表明,与单个临床上结直肠癌的血清标志物相比,与多个长链非编码RNAs的联合诊断效率更高,敏感性与特异性更强。因此,基于联合三种长链非编码RNAs与CA19-9、CEA建立的诊断模型对CRC有较高的诊断价值。
以上所述仅是本发明的优选实施方式,应当指出,对于本技术领域的普通技术人员来说,在不脱离本发明原理的前提下,还可以做出若干改进和润饰,这些改进和润饰也应视为本发明的保护范围。
序列表
<110> 遵义医学院附属医院
中国医学科学院肿瘤医院
<120> 长链非编码 RNAs的应用
<160> 11
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1752
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
ccgaggtgat caggtggact ttcctggatg ttctgggtct tgacctgatt gctgaaaaat 60
gaatacaaat tcagagaaga agaaagctag tatgagacta ccaaatgatc atcagacatt 120
tcttgaacac caattaaatt gctaggtatg ctaaagtttg caaaactggt atagacacca 180
agagggaggt atcaacagag actccccaag agctaagagg aaaccacctt ggactggaag 240
tcaagagcca aaattctaga ctctactctg taattaacta cctatttgaa tttggaaaaa 300
tcaccatcaa ctttcccagc ctcgttctct gcatctggga aatgaaggcg tcgtccaaat 360
gattacaagc ttcttttcct gctcttattg catgattcca cttccacagc cctccagcat 420
tttttagcag ctgcatcgct ccatagagcc tgcagagggc actagactgg gaattagaaa 480
acctgatttc ccttccagct ccacctctga ccaattgcct gaccctggtc aaattgctta 540
acctcttcct atctcagctc cctatccata aaacagaggg acgaataaac tctcctccta 600
ccactaagag gtgtagccag agttaatacc ctcatcgtcc tttgagctca gcagatgaaa 660
ggcactgaga aaagtacaaa gaatttttat gtgctattga ctttatttta ttttatgtgg 720
gggagggagc cggccccagc tggaaagctg ctttctctga atcaaagggc aggaacccag 780
caagtttctc aggattgggg ccttagactg ggctgtgtat acagacagtg ccagccaacc 840
ccacagttca gtttccttta acctggtgct ccaggcaata actgtgcaac tctgcaattt 900
aacaatgtgt tctttgtccc acaactgttc tcgtttctca actgcccagg taatatgttt 960
gggcctgtag gaagagtcaa atagttaata agggaagggt ttggcatgcc ctacgtaagt 1020
tctaccagca agtcccaaca agaaggcatt ctgtgtctcc tgattcctga cctaccccca 1080
aaatgtacaa atgtacaagg aatgagccca ctttcccagc aggctgtaat accagtttgg 1140
cctatatcaa tgcattggtg agctgtgttt tgtttatggt tttatgccat ctattttccc 1200
atggatatta tgttttctaa agagccctta agtttacgtc agcttttaaa gctaccagca 1260
gcaccatttc agttcatatt aagcccttaa tatggtatga ataggagagc tattagacta 1320
aagagccata atcatccctg aggaaaacat ccatcaccaa catttatgtg gtccctgaac 1380
ttctaaaagg tgtcatctct ctggggtgta tctggtgaga gctttctctg ggagatgcca 1440
aaaagccaat gcattagatg aagcttagaa gggcattttc taaccattac aaattgccta 1500
gtctagcatc tcaatttcat ctacgtgaag agccttaatt aaatttgttg gggtttgatc 1560
ctttatcccc agatgtggcg ctgacagaga ttgcttacat aaataatgtg tgctccaagt 1620
gcttgccagg ctcctggctc agctgggaca gctgtagctt tttgaatgtc attcccaaga 1680
tatcctgcag gtgttcagct tcccctgttc tactctggga agagagccgt gggcaacatc 1740
agcccagaag ac 1752
<210> 2
<211> 2265
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
aaggggcgat gcgccccgga gggggaggga cgcaaggggc gactactgtt acctcgcttg 60
gagataccaa ggccggtcgg accccccgct ggccgaatgt ggggagcgag cagatccagc 120
ctgtttggac gtggtgagga cccagccgtg gggtttaagg ccaagttagc ctctcccttc 180
actgattaca agagccattg ttacaggtgc tcacatgcca ggagaaataa gctggtgatt 240
tatgtggcac agcaccatcg atcgacctac aaaggaagct tcagtgcagc cgtgttgcta 300
tacagacctc tctcaccaag gcgtcatgaa ctgaagggct gggagaggcc tcgtggagga 360
ggaagagttt cgctcagctg cttgggtgtg ggactgaatg tggttccaga actggtcgct 420
gccttcaccc gctgactctg gtctcggcga tgacaagccc atggaacaga cttacggcgt 480
atgacctcat cggagacttg tacatggggc caacccccat tttcccacag gaaacccaca 540
ctctaattaa gggctgaccc tgaacttgaa tctgagtctt ctctttcttc aacagagagc 600
catctctcca gcccagctgg caaggagaca ggtgatgtta caggaaaaaa tacacagctt 660
gtgcccttgg aattaccaga gttcaaagcc tgttcagcat ctacccacca tgtgacccgg 720
gtcgaattct tcagtttctc taagcccatt ttctcatctg taagatgggg aagtgtaatc 780
agttctgaac ccacatatac ctcactgggc ttgaacaggc ctgagcaggc atgtggggct 840
ttactagcta gccatccttt ctgccgctct ccctcattct ccttcaagcc ccccaaagtc 900
cttggtggag gaggaagtgg agacagaacg tttggtgttg ggtgaggtac gtgaggaggc 960
tgatttcaga acctccagga aacaaagctt tagtgttccg agggcagtgc ccgcctgtgc 1020
ctcctcctca cagggggtag taggagtggg gtggggccga ggcatttccc agcacagctc 1080
aatcacatct cctgcgcctg cccttgtgac ctgctcactg gaaaggaaga aatgaccgag 1140
ggagaaggga gggcatttga ggggagaaga agaggatgga gaaaaaaact ggatcatctc 1200
gtgacatgga aagaaaggtc tgagctctga cgatcccctc acgtgattat aggcatgagg 1260
actcactctt cgaattgttt gaaaattgaa tggactggct cacaccttct gaaatttctt 1320
ggaagctctg gagtaactat tccactaaag aacagcgtgt ttttggctga aatctgtgac 1380
tgtttgtttt tgagttattt taatctctgt gtcaagcatg aaaacctcag cagcctggga 1440
tttataatta gatcccactg gcatgccctc tcacctctca aacatgccac agtgctttgg 1500
ctctctcctt gtgcaaacct ccctttgata tttttggata tcgataccaa gtgtatctgc 1560
atattttaac caactgatat gcatatcctg tgatctgagg cattcgctca atgtggggca 1620
tcaggccagc tgtgaacttc ctttaaaatt tattttgtgt caaatgattc cctaatgcta 1680
tagctccctt gaactgctag atttatgaaa attctgcaca acacatagct cagagaaaaa 1740
aaaaaaagag tgttgcaaga ccagatatag gatatcattt gatttgttta aaacactctg 1800
tccagggggt tttgacttgc taatttaaaa ataattctaa aattaggtac agttgaccct 1860
tagtatccat gggggattgg ttccaggatg ccctgtggat accagaatcc aaggatgctc 1920
aagttcctga cataaatggc ttagtgtttg catataacct actcccctct tcctatatac 1980
tttaaataat ctctagatta cttataatgc ctaacacaac gcctgtgcat cacttcattc 2040
atgtggattc aatgtagtac ttggtgtgaa gaaaatttaa gtcttgcttt ttggaacttt 2100
gtggattttt ttttctggaa tgtttttgat ctgcagttgg ttgaatccac aggtgcagaa 2160
ctatggacac agggggctga ctatgtatat ttatatccac ttgcaaaatt aagaataaaa 2220
ataatgtgca tcagcaggtg cagattctga tgctcggtgg acagt 2265
<210> 3
<211> 1154
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
ctgatgtaac agccttggga aagaggttgc agtgaaaagc tggtcctgct gtggtggaga 60
gaatggagga aagataataa aaggccaaac ctttgctcca actttctcct tagcttccct 120
ttggatctgg aaagctgggg acccacacgg cagagccatg gtactggagg agccattaac 180
aaagctttca ataaacctct ctttcttgaa gttacctgag aatggatcca ttccctgcaa 240
ctgaagattc taaggaactg ggtttctcag tatacaatgg gaatggttgg gaggaggtaa 300
agagtagaag acagtatcaa gaatccagag cccagcacct gtagtcctaa ctattcagat 360
tccttgagcc caggagtttg agtccagcct ggacaacata ttgagacccc catctctcta 420
aaaaaaaaga gaaagaaaga aggaaagaaa aaaagaaaga aagaaagaaa gaaagaaaga 480
aagaaagaaa gaaagaaaga gaaagaaaga aggaaagaag gaaagaagga aagaaagaaa 540
gaaagagaaa gaaagaaaag aagattgtag ctagggggag agtaggtgaa aagatgaaca 600
acatgaccgg gaagatttcc taatctcacc acagcctggc tctaccttaa gtctttaata 660
aaagcttgac tgaaggtacc aaggtgtgct gaagtggaag caaagttctc caaagtccag 720
catggtagac atcagtggtg gtaaccaagg acagacccca aggcaaggtg aacctcaaaa 780
atggaacctc aagtctatgc agtccagctg ccctccccac cagaaagtcc ttgttccagc 840
ccaacatcag tgcctctgag tttgtttact agaaacaaag gaagaatttc cttgtaaaaa 900
tatagacaga gtagtccctg gctttctcct cttgcaggaa ggatggattc tcccattcca 960
taccatcttt cccccacact ggccccagaa atacttaatt caactatgtg aaaataaaga 1020
ttgtttttgg tttgagggca tagggatcca tttatcctta ttctttatga ggcactaaat 1080
tagctttgta tgttattaaa tgtgtctcgt caatgctgtt ggcattgttt cattttaaaa 1140
aaaaaaaaaa aaaa 1154
<210> 4
<211> 22
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
ccctggtcaa attgcttaac ct 22
<210> 5
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
ttattcgtcc ctctgtttta tggat 25
<210> 6
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
tcctcacagg gggtagtagg 20
<210> 7
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
cccttctccc tcggtcattt 20
<210> 8
<211> 20
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
gctgtggtgg agagaatgga 20
<210> 9
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
ccccagcttt ccagatccaa a 21
<210> 10
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
cctggtatga caacgaattt g 21
<210> 11
<211> 21
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
cagtgagggt ctctctcttc c 21