CN108739058A - A kind of preparation method of Se-rich lucid ganoderma culture medium - Google Patents
A kind of preparation method of Se-rich lucid ganoderma culture medium Download PDFInfo
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- CN108739058A CN108739058A CN201810451670.3A CN201810451670A CN108739058A CN 108739058 A CN108739058 A CN 108739058A CN 201810451670 A CN201810451670 A CN 201810451670A CN 108739058 A CN108739058 A CN 108739058A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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Abstract
The invention discloses a kind of preparation methods of Se-rich lucid ganoderma culture medium, include the following steps：Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then it is 10-30 parts of 30-50 parts of corncob, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, 0.2-0.4 parts of zinc sulfate, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water by weight ratio, and it is mixed with Selenium-rich nutrient solution, after supply moisture to 1000 milliliters, obtain Se-rich lucid ganoderma culture medium.The present invention provides sufficient selenium source to ganoderma lucidum growth, the content of Organic Selenium is high in culture medium, ensure that enough Organic Seleniums can be absorbed in ganoderma lucidum growth course, improve the selenium content of ganoderma lucidum, improve the nutritive value and medical value of ganoderma lucidum, selenium content in ganoderma lucidum can not be effectively improved by solving the prior art, the problem of to cannot be satisfied use demand.
Description
Technical field
The present invention relates to ganoderma lucidum culture technique field, specially a kind of preparation method of Se-rich lucid ganoderma culture medium.
Background technology
Ganoderma lucidum is also known as Lin Zhongling, fine jade treasure is the fructification of On Polyporaceae ganoderma lucidum.With invigorating qi for tranquilization, it is relieving cough and asthma,
The effect of promoting longevity.For dizziness egersis, palpitation, neurasthenia, consumptive disease cough and asthma.It is distributed mainly on Zhejiang Province, China, black
The ground such as Longjiang, Jilin, Anhui, Jiangxi, Hunan, Guizhou, Guangdong, Fujian.The size and metamorphosis of ganoderma lucidum are very big, Large scale individual
Cap be 20 × 10 centimetres, about 2 centimetres thick, general individual is 4 × 3 centimetres, 0.5-1 centimetres thick, there is numerous small holes below, is managed
Mouth is white or filbert, has 4-5 in every millimeter, nozzle is round, and inner wall is hymenium, and spore results from load top.
Selenium (Se) is the high-efficient oxidant in human body, has and improves a variety of biologies such as immunity of organisms, antitumor, anti-aging
Learn function.1973, WHO and international nutritive issue confirmed it for required trace element in human body and animal body.But full generation
Boundary has nearly 50 countries and regions to have the problem of selenium deficiency, the problem can be made to be resolved by producing Se-rich farm products.
Early in 1984, Finland just utilized the fertilizer production cereal containing sodium selenate and successful.The beginning of the nineties, China various regions also land
It is continuous to start Se-rich farm products production.Se-rich farm products production as a result, experienced one from inefficient dispersal to the process efficiently concentrated.
China's Se-rich farm products have begun to take shape at present, mainly there is more than 30 a kind such as selenium-enriched edible mushroom, selenium-enriched rice, selenium-enriched tea leaf.
Wherein, the development of selenium-enriched edible mushroom has clear superiority, and existing ganoderma lucidum Se content is relatively low, and the prior art can not effectively improve spirit
Selenium content in sesame, to cannot be satisfied use demand, for this purpose, it is proposed that a kind of preparation method of Se-rich lucid ganoderma culture medium.
Invention content
The purpose of the present invention is to provide a kind of preparation methods of Se-rich lucid ganoderma culture medium, to solve in above-mentioned background technology
The problem of proposition.
To achieve the above object, the present invention provides the following technical solutions：A kind of preparation method of Se-rich lucid ganoderma culture medium, packet
Include following steps：Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-
BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then by weight ratio be corncob
10-30 parts of 30-50 parts, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, zinc sulfate 0.2-0.4
Part, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and sought with selenium-rich
Nutrient solution mixes, after supply moisture to 1000 milliliters, obtain Se-rich lucid ganoderma culture medium, tubulature (bottle) sterilizing is spare, and culture medium is filled
It sterilizes immediately after good, miscellaneous bacteria therein is killed, lucidum strain is then accessed, is fermented and is cultivated.
Preferably, the ganoderma lucidum selenium content of the selenium-rich culturing base production is l80-280mg/g.
Preferably, in 6.5-7, high pressure 0.15MPa is kept under the conditions of 127 DEG C of temperature for pH value control in the incubation
2 hours.
Preferably, the selenium salt uses sodium selenate, one kind in sodium selenite and calcium selenate.
Preferably, for the fermentation humid control in 60%-70%, the bacterium germination time is 20-40 days.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention is added to Selenium-rich nutrient solution, on the one hand gives ganoderma lucidum
Normal growth provides sufficient nutriment, and on the other hand, the 6-BA basic elements of cell division in Selenium-rich nutrient solution can promote carefully
Born of the same parents' division, the formation of induced bud simultaneously promote growth, by adding selenium-rich fertilizer and selenium salt, are not only provided fully to ganoderma lucidum growth
Selenium source, and the selenium source is safe and reliable, and the content of Organic Selenium is high in culture medium, so that it is guaranteed that can be inhaled in ganoderma lucidum growth course
Enough Organic Seleniums are received, the selenium content in ganoderma lucidum body is improved, improves the nutritive value and medical value of ganoderma lucidum, solve existing skill
Art can not effectively improve the selenium content in ganoderma lucidum, the problem of to cannot be satisfied use demand.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this field
The every other embodiment that art personnel are obtained without making creative work belongs to the model that the present invention protects
It encloses.
The present invention provides a kind of technical solution：A kind of preparation method of Se-rich lucid ganoderma culture medium, includes the following steps：First
Constituent is by weight ratio：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-BA0.1-0.2 parts, selenium-rich
0.2-0.4 parts fertile, 1000 parts of water, obtains Selenium-rich nutrient solution, is then 30-50 parts of corncob, straw powder 20- by weight ratio
10-30 parts of 40 parts, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, 0.2-0.4 parts of zinc sulfate, l-2 parts of lime, salt
L-2 parts, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and mixed with Selenium-rich nutrient solution, after supply water
Dividing to 1000 milliliters, obtains Se-rich lucid ganoderma culture medium, tubulature (bottle) sterilizing is spare, sterilizes immediately after culture medium is installed,
Miscellaneous bacteria therein is killed, lucidum strain is then accessed, fermented and is cultivated.
Embodiment one：
Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-
BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then by weight ratio be corncob
10-30 parts of 30-50 parts, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, zinc sulfate 0.2-0.4
Part, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and sought with selenium-rich
Nutrient solution mixes, after supply moisture to 1000 milliliters, obtain Se-rich lucid ganoderma culture medium, tubulature (bottle) sterilizing is spare, and culture medium is filled
It sterilizes immediately after good, miscellaneous bacteria therein is killed, lucidum strain is then accessed, is fermented and is cultivated.
Embodiment two：
In embodiment one, following processes are added：
The ganoderma lucidum selenium content of selenium-rich culturing base production is l80-280mg/g.
Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-
BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then by weight ratio be corncob
10-30 parts of 30-50 parts, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, zinc sulfate 0.2-0.4
Part, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and sought with selenium-rich
Nutrient solution mixes, after supply moisture to 1000 milliliters, obtain Se-rich lucid ganoderma culture medium, tubulature (bottle) sterilizing is spare, and culture medium is filled
It sterilizes immediately after good, miscellaneous bacteria therein is killed, lucidum strain is then accessed, is fermented and is cultivated, wherein selenium-rich is trained
The ganoderma lucidum selenium content for supporting base production is l80-280mg/g.
Embodiment three：
In embodiment two, following processes are added：
PH value control is in 6.5-7 in incubation, and high pressure 0.15MPa is kept for 2 hours under the conditions of 127 DEG C of temperature.
Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-
BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then by weight ratio be corncob
10-30 parts of 30-50 parts, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, zinc sulfate 0.2-0.4
Part, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and sought with selenium-rich
Nutrient solution mixes, after supply moisture to 1000 milliliters, obtain Se-rich lucid ganoderma culture medium, pH value control is high in 6.5-7 in incubation
0.15MPa is pressed to be kept for 2 hours under the conditions of 127 DEG C of temperature, tubulature (bottle) sterilizing is spare, goes out immediately after culture medium is installed
Miscellaneous bacteria therein is killed, then accesses lucidum strain by bacterium, is fermented and is cultivated, wherein the ganoderma lucidum of selenium-rich culturing base production
Selenium content is l80-280mg/g.
Example IV：
In embodiment three, following processes are added：
Selenium salt uses sodium selenate, one kind in sodium selenite and calcium selenate.
Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-
BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then by weight ratio be corncob
10-30 parts of 30-50 parts, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, zinc sulfate 0.2-0.4
Part, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and sought with selenium-rich
Nutrient solution mixes, and selenium salt uses sodium selenate, one kind in sodium selenite and calcium selenate, after supply moisture to 1000 milliliters, obtain richness
Selenium Medium for Ganoderma lucidum, in 6.5-7, high pressure 0.15MPa is kept for 2 hours under the conditions of 127 DEG C of temperature for pH value control in incubation,
Tubulature (bottle) sterilizing is spare, sterilizes immediately after culture medium is installed, miscellaneous bacteria therein is killed, Ganoderma Lucidum is then accessed
Kind, it is fermented and is cultivated, the ganoderma lucidum selenium content of wherein selenium-rich culturing base production is l80-280mg/g.
Embodiment five：
In example IV, following processes are added：
Humid control ferment in 60%-70%, the bacterium germination time is 20-40 days.
Constituent is by weight ratio first：10-l5 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-
BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, 1000 parts of water, obtain Selenium-rich nutrient solution, then by weight ratio be corncob
10-30 parts of 30-50 parts, 20-40 parts of straw powder, rice bran or wheat bran, l-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, zinc sulfate 0.2-0.4
Part, l-2 parts of lime, l-2 parts of salt, 0.1-0.3 parts of potassium dihydrogen phosphate, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and sought with selenium-rich
Nutrient solution mixes, and selenium salt uses sodium selenate, one kind in sodium selenite and calcium selenate, after supply moisture to 1000 milliliters, obtain richness
Selenium Medium for Ganoderma lucidum, in 6.5-7, high pressure 0.15MPa is kept for 2 hours under the conditions of 127 DEG C of temperature for pH value control in incubation,
Tubulature (bottle) sterilizing is spare, sterilizes immediately after culture medium is installed, miscellaneous bacteria therein is killed, Ganoderma Lucidum is then accessed
Kind, it is fermented and is cultivated, the ganoderma lucidum selenium content of wherein selenium-rich culturing base production is l80-280mg/g, and fermentation humid control exists
60%-70%, bacterium germination time are 20-40 days.
The present invention is added to Selenium-rich nutrient solution, on the one hand provides sufficient nutriment to ganoderma lucidum normal growth, another
Aspect, the 6-BA basic elements of cell division in Selenium-rich nutrient solution can promote cell division, the formation of induced bud and promote growth, lead to
Addition selenium-rich fertilizer and selenium salt are crossed, not only sufficient selenium source is provided to ganoderma lucidum growth, but also the selenium source is safe and reliable, in culture medium
The content of Organic Selenium is high, so that it is guaranteed that can absorb enough Organic Seleniums in ganoderma lucidum growth course, improves in ganoderma lucidum body and contains selenium
Amount, improves the nutritive value and medical value of ganoderma lucidum, and selenium content in ganoderma lucidum can not be effectively improved by solving the prior art, to
The problem of cannot be satisfied use demand.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (5)
1. a kind of preparation method of Se-rich lucid ganoderma culture medium, it is characterised in that：Include the following steps：Constituent is by weight first
Part matches：10-15 parts of glucose, 80-100 parts of bio-azotobacter fertilizer, 6-BA0.1-0.2 parts, 0.2-0.4 parts of selenium-rich fertilizer, water
1000 parts, Selenium-rich nutrient solution is obtained, is then 30-50 parts of corncob, 20-40 parts of straw powder, rice bran or wheat by weight ratio
10-30 parts of bran, 1-2 parts of gypsum, 0.5-1 parts of magnesium sulfate, 0.2-0.4 parts of zinc sulfate, 1-2 parts of lime, 1-2 parts of salt, di(2-ethylhexyl)phosphate
0.1-0.3 parts of hydrogen potassium, 0.1-0.5 parts of selenium salt, 60-80 parts of water, and mixed with Selenium-rich nutrient solution, after supply moisture to 1000 millis
It rises, obtains Se-rich lucid ganoderma culture medium, tubulature (bottle) sterilizing is spare, sterilizes immediately after culture medium is installed, will be therein miscellaneous
Bacterium kills, and then accesses lucidum strain, is fermented and cultivated.
2. a kind of preparation method of Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that：The selenium-rich culturing
The ganoderma lucidum selenium content of base production is 180-280mg/g.
3. a kind of preparation method of Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that：The incubation
In 6.5-7, high pressure 0.15MPa is kept for 2 hours the control of middle pH value under the conditions of 127 DEG C of temperature.
4. a kind of preparation method of Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that：The selenium salt uses
Sodium selenate, one kind in sodium selenite and calcium selenate.
5. a kind of preparation method of Se-rich lucid ganoderma culture medium according to claim 1, it is characterised in that：The fermentation humidity
In 60%-70%, the bacterium germination time is 20-40 days for control.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106431572A (en) * | 2016-09-30 | 2017-02-22 | 桂林淮安天然保健品开发有限公司 | Lucid-ganoderma cultivation method for increasing ganoderan and selenium content |
| JP2017042125A (en) * | 2015-08-28 | 2017-03-02 | 株式会社ウエルネス四万十研究所 | Cordyceps militaris having various efficacy, and production method thereof |
| CN107646531A (en) * | 2017-09-30 | 2018-02-02 | 安徽利民生物科技股份有限公司 | A kind of preparation method of Se-rich lucid ganoderma culture medium |
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- 2018-05-12 CN CN201810451670.3A patent/CN108739058A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017042125A (en) * | 2015-08-28 | 2017-03-02 | 株式会社ウエルネス四万十研究所 | Cordyceps militaris having various efficacy, and production method thereof |
| CN106431572A (en) * | 2016-09-30 | 2017-02-22 | 桂林淮安天然保健品开发有限公司 | Lucid-ganoderma cultivation method for increasing ganoderan and selenium content |
| CN107646531A (en) * | 2017-09-30 | 2018-02-02 | 安徽利民生物科技股份有限公司 | A kind of preparation method of Se-rich lucid ganoderma culture medium |
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Application publication date: 20181106 |