CN108731805B - Absorption and fluorescence spectrum detection device based on mobile intelligent terminal - Google Patents
Absorption and fluorescence spectrum detection device based on mobile intelligent terminal Download PDFInfo
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- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
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- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
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- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
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Abstract
The invention discloses an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal, which comprises the mobile intelligent terminal, an optical sensing accessory and an adapter, wherein the optical sensing accessory is arranged on the mobile intelligent terminal through the adapter; the spectrum acquisition module, the colorimetric module and the fluorescent module in the mobile intelligent terminal can finally obtain the concentration of the solution to be detected according to the photo shot by the CCD camera, and the absorption and fluorescent spectrum detection device is light and portable in structure, simple to operate and low in price, and can realize real-time on-site and high-precision multi-mode rapid spectrum detection of the object to be detected by utilizing two detection modes of colorimetric and fluorescent.
Description
Technical Field
The invention relates to the technical field of spectrometers, in particular to an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal.
Background
With the development of computer technology, mobile intelligent terminals (such as smartphones, tablet computers, etc.) have gradually become an indispensable tool in people's lives. The mobile intelligent terminal has the functions of the computer due to the high-performance CPU, and can realize the functions of a general large instrument by adding some simple accessories.
With the demands of people for real-time detection, the spectrum detection has wide application and clear industrialization prospect in the fields of food detection, environment monitoring, disease diagnosis, clinical monitoring and the like. Conventional spectrum detection techniques cannot be directly used for on-site rapid detection due to the defects of heavy system volume, high price, complex operation and the like. For this deficiency, some miniature spectrometers have been developed by companies or teams represented by the united states ocean optics, netherlands love Mo Disi, japan bingo, etc. Although the palm size and price of the equipment can be achieved, the equipment is still an independent and complete hardware system, including a light source, a circuit, an external display device and the like, and the real portability cannot be realized. Fortunately, the high-resolution camera of the mobile intelligent terminal can shoot visible light spectrum, the terminal screen can be used as display equipment, and the spectrum detection device based on the mobile intelligent terminal platform can be realized only by combining a light source related module with the mobile intelligent terminal and assisting with terminal software. Such a spectrum sensing device has lower cost and better portability than currently popular miniature spectrometers. In addition, through the mobile internet, the spectrum detection device can directly upload data without additional network equipment.
However, the current spectrum detection device based on the mobile intelligent terminal can only realize a single detection mode, such as colorimetric detection or fluorescence detection, and the single detection mode has the defects of low sensitivity, small detection range, poor specificity and the like, so that the application of the spectrum detection device in the field rapid detection is limited. The detection modes detect simultaneously, and can correct the detection results mutually to ensure the accuracy of the detection results, so that the requirement of realizing real-time and multi-mode detection on site is urgently met.
Disclosure of Invention
The invention provides an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal, which can perform real-time in-situ and high-precision multi-mode rapid spectrum detection.
An absorption and fluorescence spectrum detection device based on a mobile intelligent terminal, comprising: the mobile intelligent terminal comprises a CCD camera, a touch display screen and a central processing unit, an optical sensing accessory and an adapter for installing the optical sensing accessory on the mobile intelligent terminal; the optical sensing accessory comprises a first lamp source used for a colorimetric detection mode and a second lamp source used for a fluorescent detection mode, and an emergent light path I of the first lamp source is perpendicular to an emergent light path II of the second lamp source; the optical sensing accessory further comprises a cuvette, the cuvette is coaxial with the first emergent light path and the second emergent light path, the first light source is a full-spectrum light source, the emergent light of the second light source excites the solution in the cuvette to generate fluorescence with wavelength required by detection, the optical sensing accessory further comprises a reflector arranged at the rear end of the cuvette and along the first emergent light path, the first emergent light path and the reflector have an incident angle, the optical sensing accessory further comprises a diffraction grating arranged between the CCD camera and the reflector, the diffraction grating is vertically incident after the emergent light passing through the cuvette and along the first emergent light path is reflected by the reflector, and the diffraction grating is used for diffracting the solution on the CCD camera, and the optical sensing accessory further comprises a light source control module which is connected with each light source circuit and respectively controls the opening and closing of each light source;
the mobile intelligent terminal comprises a main menu module, a spectrum acquisition module, a colorimetric module, a fluorescent module and a concentration display module; the main menu module sends a detection mode selection instruction to the light source control module, the light source control module selects the first light source or the second light source to work according to the detection mode selection instruction, when the first light source or the second light source works, the main menu module sends a spectrum acquisition instruction to the spectrum acquisition module when the cuvette is not added with the solution to be detected or is added with the solution to be detected in two states, the spectrum acquisition module controls the CCD camera of the mobile intelligent terminal to take a picture according to the spectrum acquisition instruction, draws the light intensity into a spectrogram according to the relation between the wavelength and the pixel, sends the spectrogram to the colorimetric module or the fluorescent module according to the detection mode selection, the colorimetric module acquires the light intensities of two specific wavelengths in the state of not adding the solution to be detected or the solution to be detected according to the spectrogram, acquires the absorbance of the solution to be detected at the two specific wavelengths according to a beer's specific formula, acquires the absorbance of the solution to be detected at the two specific wavelengths according to the ratio of the absorbance at the two specific wavelengths and the solution to be detected in the state of not adding the solution to be detected, and acquires the relative concentration of the solution to be detected in the state of the linear state of not changing the relative to the concentration of the solution to be detected in the mobile intelligent terminal.
In the invention, an optical sensing accessory is arranged on a mobile intelligent terminal by an adapter, a user can select two detection modes of colorimetric and fluorescence, when the colorimetric detection mode is selected, a first light source is started, when the fluorescent detection mode is selected, a second light source is started, emergent light of the second light source can excite a solution in a cuvette to generate fluorescence, light which is transmitted through the cuvette along an emergent light path is reflected on a reflecting mirror, reflected light irradiates a diffraction grating, and a spectral band is formed through the spectral action of the diffraction grating to reach a CCD camera; the spectrum acquisition module, the colorimetric module and the fluorescent module in the mobile intelligent terminal can finally obtain the concentration of the solution to be detected according to the photo shot by the CCD camera, so that the absorption and fluorescent spectrum detection device based on the mobile intelligent terminal is light and portable in structure, simple to operate and low in price, and can realize real-time, on-site and high-precision multi-mode rapid spectrum detection of an object to be detected by utilizing two detection modes of colorimetric and fluorescent.
Drawings
Fig. 1 is a schematic structural diagram of an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal in an embodiment of the invention;
FIG. 2 is a schematic diagram of an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal according to an embodiment of the present invention;
FIG. 3 is a flow chart of calibration between wavelength and pixels of an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal in an embodiment of the invention;
fig. 4 is a detection flow chart of an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal in an embodiment of the invention.
Detailed Description
The embodiment of the invention provides an absorption and fluorescence spectrum detection device based on a mobile intelligent terminal, which is described in detail below.
In this embodiment, the mobile intelligent terminal is a smart phone, and the solution to be measured is a nano gold reaction solution.
Referring to fig. 1, the mobile intelligent terminal-based absorption and fluorescence spectrum detection device comprises a smart phone 01, an adapter 02 and an optical sensing accessory 03, wherein the optical sensing accessory 03 is fixed on the smart phone 01 through the adapter 02.
Referring to fig. 2, the smart phone 01 has a CCD camera 9, a display screen 10 and a central processing unit, and the optical sensing accessory 03 includes a first light source 1 for colorimetric detection mode and a second light source 2 for fluorescent detection mode, wherein an outgoing light path of the first light source 1 is perpendicular to an outgoing light path of the second light source 2; the optical sensing accessory 03 further comprises a cuvette 3, the cuvette 3 is coaxial with the first emergent light path and the second emergent light path, emergent light of the first lamp source 1 is transmitted out of the cuvette 3, the first lamp source 1 is a full-spectrum light source, the emergent light of the second lamp source 2 excites solution in the cuvette 3 to generate fluorescence with a wavelength required for detection, the fluorescence can also transmit out of the cuvette 3 along one direction of the emergent light path, the optical sensing accessory 03 further comprises a reflector 6 arranged at the rear end of the cuvette 3 and along the first emergent light path, an incident angle exists between the first emergent light path and the reflector 6, the optical sensing accessory 03 further comprises a diffraction grating 8 arranged between the CCD camera 9 and the reflector 6, the emergent light passing through the cuvette 3 and the first emergent light path is reflected by the reflector 6 and then perpendicularly incident on the diffraction grating 8, the solution is diffracted on the CCD camera 9 through the diffraction grating 8, and the optical sensing accessory 03 further comprises a lamp source control module which is connected with the first lamp source 1 and the second lamp source 2 in a circuit and is used for controlling the opening and closing of each lamp source respectively.
Here, the light emitted through the cuvette 3 and along the first light path may be the light emitted by the first light source 1 or the fluorescence excited by the second light source 2.
It should be noted that, the first light path of the first light source 1 and the second light path of the second light source 2 are perpendicular to each other, because the fluorescent signal of the solution to be measured is weaker than the excitation light, so as to reduce the interference of the excitation light.
The diffraction grating 8 is used to split the reflected light of the reflecting mirror 6 by the diffraction grating 8 when the light source is a full spectrum light source.
In this embodiment, optionally, the optical sensing accessory 03 further includes an aperture-adjustable diaphragm 4, a first focusing lens 5 and a second focusing lens 7, where the light transmitted through the cuvette is too strong and affects the sensitivity of the detection device, in order to improve the sensitivity of the detection device, the diaphragm 4 is used to control the light entering the CCD camera 9 of the mobile phone, the first focusing lens 5 is used to reduce the loss of the light emitted from the first light source 1 on the reflecting mirror 6, and by focusing the light transmitted through the diaphragm 4, the light can be focused on the surface of the reflecting mirror 6, the second focusing lens 7 is used to focus the reflected light of the reflecting mirror 6 on the surface of the diffraction grating 8 due to the influence of the diffraction quality of the grating by the shape of the incident light, and specifically: a pluggable aperture 4, a pluggable focusing lens I5 and light transmitted through the focusing lens I5 are sequentially arranged between the cuvette 3 and the reflecting mirror 6, the reflected light is reflected through the focusing lens II 7 and the diffraction grating 8, and the reflected light reaches a CCD camera 9 of the smart phone 01.
In this embodiment, alternatively, the first light source 1 is a full spectrum LED lamp, and the second light source 2 is a LED lamp or a laser lamp with a specific wavelength.
In this embodiment, optionally, the first focusing lens 5 and the second focusing lens 7 are plano-convex lenses, each having a thickness of 1mm, a diameter of 10mm, and focal lengths of 25mm and 15mm, respectively; the reflector 6 is a plane reflector, the thickness is 1mm, and the diameter is 10mm; the diffraction grating 8 is a transmission grating, the thickness is 2mm, the diameter is 10mm, and the line of the diffraction grating is not less than 1200lines/mm.
In this embodiment, optionally, for convenient cleaning of the cuvette 3, the optical sensing accessory 03 further includes a sample groove, and the cuvette 3 is clamped in the sample groove, so that the cuvette 3 can be flexibly taken out or put in.
The optical sensing accessory 03 further comprises a lamp source control module, and the lamp source control module is respectively connected with the first lamp source 1 and the second lamp source 2 in a circuit mode. Optionally, the first light source 1 and the second light source 2 are also connected with a USB interface circuit of the mobile phone, and the built-in battery of the mobile phone supplies power for the first light source 1 and the second light source 2.
The intelligent mobile phone comprises a main menu module, a spectrum acquisition module, a colorimetric module, a fluorescence module, a concentration display module and a network sharing module.
The main menu module sends a detection mode selection instruction to the light source control module, the light source control module controls the light source I1 or the light source II 2 to work according to the detection mode selection instruction, when the light source I1 or the light source II 2 works, the light source II is in a state that the nano gold reaction solution is not added into the cuvette 3 or is added into the nano gold reaction solution, in other words, the light source is in a state that: in the colorimetric detection mode, the first light source 1 works, in the two states that no nano gold reaction solution is added to the cuvette 3 or the nano gold reaction solution is added to the cuvette 3, or in the fluorescent detection mode, the second light source 2 works, in the two states that no nano gold reaction solution is added to the cuvette 3 or the nano gold reaction solution is added to the cuvette 3, the main menu module sends a spectrum acquisition instruction to the spectrum acquisition module, the spectrum acquisition module controls the CCD camera 9 of the smart phone to take a picture according to the spectrum acquisition instruction, the light intensity of each pixel in the picture is obtained, the light intensity is drawn into a spectrogram according to the relation between the wavelength and the pixel, and the spectrogram is sent to the colorimetric module or the fluorescent module according to the detection mode.
When the detection mode is selected to be the colorimetric mode, the colorimetric module obtains the light intensities of two specific wavelengths in the two states without adding the nano-gold reaction solution or with adding the nano-gold reaction solution according to the spectrogram, namely, the colorimetric module obtains the wavelengths of 520nm and 625nm in the two states without adding the nano-gold reaction solution or with adding the nano-gold reaction solution according to the spectrogramThe light intensity is calculated according to the formula of lambert beer law: obtaining absorbance A of the gold nanoparticle reaction solution corresponding to 625nm wavelength 625 And absorbance A of the nanogold reaction solution corresponding to 520nm wavelength 520 Further, the absorbance ratio A is obtained 625 /A 520 Since the ratio A is within a certain concentration range 625 /A 520 The concentration of the object to be measured is obtained according to the linear relation between the ratio and the concentration of the object to be measured.
When the detection mode is selected to be the fluorescence mode, the fluorescence module acquires the relative change value of the light intensity at 517nm wavelength in the spectrogram under the two states of no addition of the solution to be detected or the addition of the solution to be detected, and the relative change value is linearly increased along with the increase of the concentration of the object to be detected in a certain concentration range, so that the concentration of the object to be detected is acquired according to the linear relation between the relative change value and the concentration of the object to be detected.
The concentration display module displays concentration information on the mobile phone display screen 10 according to the concentration sent by the colorimetric module or the fluorescent module, and sends the corresponding concentration to the network sharing module, and the network sharing module receives the concentration and distributes the concentration on a network.
In this embodiment, the adapter installs the optical sensing accessory on the smart phone, the user can select two detection modes of colorimetric and fluorescence, when selecting colorimetric detection mode, the first light source is turned on, when selecting fluorescence detection mode, the second light source is turned on, the emergent light of the second light source can excite the solution in the cuvette to generate fluorescence, the light emitted by the first light source 1 and the fluorescence generated by the solution excited by the second light source 2 pass through the cuvette 3, the aperture 4, the focusing lens 5, the reflecting mirror 6, the focusing lens 7 and the diffraction grating 8, and then reach the CCD camera 9, the spectrum acquisition module, the colorimetric module and the fluorescent module in the smart phone can finally obtain the concentration of the solution to be detected according to the photo shot by the CCD camera, so that the device based on the absorption and fluorescence spectrum detection of the mobile smart terminal is light and portable in structure, is simple to operate and low in price, and real-time, high-precision multi-mode rapid spectrum detection can be realized on the object to be detected by utilizing the two detection modes of colorimetric and fluorescence.
Referring to fig. 3, the absorption and fluorescence spectrum sensing device in the present embodiment also has a correction function capable of correcting the relationship between the wavelength and the photo pixel before the sensing is performed, and referring to fig. 3, the correction process will be described in detail below.
301. Recording the pixel position P of the emergent light of the laser lamp with the wavelength of 405nm in the photo under the state that no nano gold reaction solution exists in the cuvette 405 ；
The method comprises the steps that a first light source is replaced by a full-spectrum light source, the wavelength of the full-spectrum light source is 405nm, a light source control module controls the laser to work according to a selection detection mode instruction sent by a main menu module in a mobile phone, the main menu module sends a spectrum acquisition instruction to a spectrum acquisition module in the mobile phone in a state that no nano gold reaction solution exists in a cuvette, the spectrum acquisition module controls a CCD camera of the mobile phone to take pictures, and the pixel position P of emergent light of the laser in the pictures is recorded 405 。
302. Recording the pixel position P of the outgoing light of the laser lamp with the wavelength of 450nm in the photo under the state that no nano gold reaction solution exists in the cuvette 450 ；
Replacing the laser lamp with the wavelength of 405nm with the laser lamp with the wavelength of 450nm, and executing the operation flow of the mobile phone in the step 301 to obtain the pixel position P of the outgoing light of the laser lamp with the wavelength of 450nm in the photo 450 。
303. Recording the pixel position P of the emergent light of the 532nm laser lamp in the photo under the state that no nano gold reaction solution exists in the cuvette 532 ；
Replacing the laser lamp with the wavelength of 450nm with the laser lamp with the wavelength of 532nm, and executing the operation flow of the mobile phone in the step 301 to obtain the pixel position P of the emergent light of the laser lamp with the wavelength of 532nm in the photo 532 。
304. Recording the pixel position P of the outgoing light of the 650nm laser lamp in the photo under the state that no nano gold reaction solution exists in the cuvette 650 ；
The laser lamp with the wavelength of 532nm is replaced by a laser lamp with the wavelength of 650nm, the operation flow of the mobile phone in the step 301 is executed,obtaining the pixel position P of the outgoing light of the laser lamp with the wavelength of 650nm in the photo 650 。
305. The spectrum acquisition module of the mobile phone acquires four points (405, P 405 )、(450，P 450 )、(532，P 532 ) And (650, P) 650 ) And performing linear fitting to obtain the relation between the wavelength and the pixel.
The spectrum acquisition module obtains the relation between the wavelength and the pixels, the light source is replaced by the full spectrum light source, the main menu module sends a detection mode selection instruction to the light source control module, and detection starts.
Referring to fig. 4, the detection process of the absorption and fluorescence spectrum detection device in this embodiment will be specifically described below by taking methamphetamine as an example.
In this embodiment, the process of preparing the nano gold reaction solution is as follows: firstly, adding 10 mu L of 5 mu M methamphetamine aptamer (the tail end of which is modified with a fluorescent probe excited to 495nM and emitted to 517 nM) into 120 mu L of sample solution containing methamphetamine to be detected, reacting for 5min at room temperature, adding 150 mu L of 9nM nano gold solution, reacting for 5min, and adding 20 mu L of 500nM NaCl solution.
When the absorption and fluorescence spectrum detection device adopts a colorimetric detection mode, the specific process is as follows:
401. the light source control module in the optical sensing accessory controls the first light source to work according to the instruction sent by the main menu module in the mobile phone;
the main menu module in the mobile phone sends a detection mode selection instruction to the light source control module, and the light source control module controls the first light source to work according to the detection mode selection instruction, namely the emergent light is white light with full spectrum.
402. The spectrum acquisition module acquires a spectrogram S under the two states that no nano gold reaction solution is added into the cuvette and no nano gold reaction solution is added into the cuvette 0 And a spectrogram S;
light emitted by the first light source passes through the cuvette, the aperture, the first focusing lens, the reflecting mirror, the second focusing lens and the diffraction grating and then reaches the CCD camera.
In cuvetteIn the state that the nano gold reaction solution is not added, a main menu module in the mobile phone sends a spectrum acquisition instruction to a spectrum acquisition module in the mobile phone, the spectrum acquisition module controls a CCD camera of the mobile phone to take a picture according to the spectrum acquisition instruction, the light intensity of each pixel in the picture is obtained, and the light intensity is drawn into a spectrogram S according to the relation between the wavelength and the pixels 0 。
And in a state that the nano gold reaction solution is added in the cuvette, a main menu module in the mobile phone sends a spectrum acquisition instruction to a spectrum acquisition module in the mobile phone again, the spectrum acquisition module controls a CCD camera of the mobile phone to take a picture according to the spectrum acquisition instruction, the light intensity of each pixel in the picture is obtained, and the light intensity is drawn into a spectrogram S according to the relation between the wavelength and the pixels.
403. According to spectrogram S 0 S, the colorimetric module obtains the concentration of methamphetamine;
colorimetric module in mobile phone according to spectrogram S 0 And the spectrum S to obtain the light intensity I at 520nm 0G And I G Also according to spectrogram S 0 And the spectral diagram S to obtain the light intensity I at 625nm 0R And I R Then the absorbance A of the nano gold reaction solution at the wavelength of 625nm is respectively obtained according to the lambert beer law formula 625 And absorbance A at a wavelength of 520nm 520 Further, the absorbance ratio A is obtained 625 /A 520 Finally, the concentration of methamphetamine is obtained according to the linear relation between the absorbance ratio and the concentration of methamphetamine.
The linear relationship between absorbance ratio and methamphetamine concentration is referred to as: the absorbance ratio increases linearly with the concentration of methamphetamine, and can be obtained by converting a standard curve of a linear relation between a preset ratio and the concentration in a colorimetric module.
The colorimetric module sends the concentration of methamphetamine to the concentration display module.
404. The concentration display module displays the concentration on a mobile phone screen and is distributed on a network by the network sharing module;
when the absorption and fluorescence spectrum detection device adopts a fluorescence detection mode, the specific process is as follows:
405. the light source control module in the optical sensing accessory controls the second light source to work according to the instruction sent by the main menu module in the mobile phone;
the main menu module in the mobile phone sends a detection mode selection instruction to the light source control module, the light source control module controls the second light source to work according to the detection mode selection instruction, the wavelength of emergent light of the second light source is 495nm, and the solution in the cuvette is excited to generate fluorescence.
406. In two states of adding no nano gold reaction solution and adding nano gold reaction solution in the cuvette, the spectrum acquisition module acquires a spectrogram S' 0 And S';
the fluorescence reaches the CCD camera after passing through the cuvette, the aperture, the first focusing lens, the reflecting mirror, the second focusing lens and the diffraction grating. The execution flow of step 406 is the same as the execution flow of step 402.
407. According to spectrogram S 0 And S', obtaining the concentration of methamphetamine by the fluorescent module;
the fluorescent module in the mobile phone is used for obtaining the spectrogram S' 0 And S' to obtain the light intensity F at 517nm 0 And F, obtaining a relative change value of fluorescence intensity generated by the nano gold reaction solution under the irradiation of a second lamp source, and obtaining the concentration of methamphetamine according to a linear relation between the relative change value and the concentration of methamphetamine.
The linear relationship between the relative change value and methamphetamine concentration is referred to as: the relative change value increases linearly with the increase of the methamphetamine concentration, and can be obtained by presetting a standard curve conversion of the linear relation between the relative change value and the concentration in a fluorescence module.
The fluorescence module sends the concentration of methamphetamine to the concentration display module.
Finally, the step 404 is executed, and the concentration display module displays the concentration of methamphetamine provided by the fluorescence module on the mobile phone screen, and the concentration is distributed on the network by the network sharing module.
The mobile intelligent based on the embodiment of the inventionThe absorption and fluorescence spectrum detection device of the terminal is described in detail, and specific examples are applied to illustrate the principle and the implementation mode of the invention, and the description of the above examples is only used for helping to understand the method and the core idea of the invention; also, as for the person skilled in the art, the specific embodiment and application range can be changed according to the idea of the present invention, for example, in this example, the ratio of absorbance of the nano-gold reaction solution at 625nm and 520nm can be A 520 /A 625 The linear relationship between the ratio and the concentration is: the ratio decreases linearly with increasing concentration; for another example, other fluorescent probes can be modified on the aptamer, corresponding lasers or LEDs are selected as the second light source according to the excitation wavelength of the fluorescent probes, and the relative variation value of the fluorescence intensity at the emission wavelength of the fluorescent probes is selected by the fluorescent module to calculate the concentration of the object to be detected; for another example, the invention detects the specificity of the specific nucleic acid aptamer, and by replacing the sequence of the nucleic acid aptamer, the invention can establish the specificity detection of other substances in other fields; for another example, the absorption and fluorescence spectrum detection device based on the mobile intelligent terminal can also realize colorimetric detection by means of other substances besides the nano gold particles, and the absorbance calculation can be carried out by selecting the light intensity at the wavelength corresponding to the characteristic absorption peak in the colorimetric module according to the change of the characteristic absorption peak position. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (8)
1. Absorption and fluorescence spectrum detection device based on mobile intelligent terminal, characterized by comprising: the mobile intelligent terminal comprises a CCD camera, a touch display screen and a central processing unit, an optical sensing accessory and an adapter for installing the optical sensing accessory on the mobile intelligent terminal; the optical sensing accessory comprises a first lamp source used for a colorimetric detection mode and a second lamp source used for a fluorescent detection mode, and an emergent light path I of the first lamp source is perpendicular to an emergent light path II of the second lamp source; the optical sensing accessory further comprises a cuvette, the cuvette is coaxial with the first emergent light path and the second emergent light path, the first light source is a full spectrum light source, the emergent light of the second light source excites the solution in the cuvette to generate fluorescence with a wavelength required for detection, the optical sensing accessory further comprises a reflector which is arranged at the rear end of the cuvette and along the first emergent light path, an incidence angle exists between the first emergent light path and the reflector, the optical sensing accessory further comprises a diffraction grating which is arranged between the CCD camera and the reflector, the emergent light passing through the cuvette and along the first emergent light path is reflected by the reflector and then vertically enters the diffraction grating, and is diffracted on the CCD camera through the diffraction grating, the optical sensing accessory further comprises a light source control module which is connected with each light source circuit and respectively controls the opening and closing of each light source, the optical sensing accessory further comprises a focusing lens I which is arranged between the reflector and the first emergent light path in a pluggable manner, the emergent light passing through the diffraction grating and the second emergent light path is reflected by the reflector and the reflector, and the focusing lens 38can be focused by the focusing lens 62 which is arranged between the first emergent light path and the reflector and the reflecting lens;
the mobile intelligent terminal comprises a main menu module, a spectrum acquisition module, a colorimetric module, a fluorescent module and a concentration display module; the main menu module sends a detection mode selection instruction to the light source control module, the light source control module selects the first light source or the second light source to work according to the detection mode selection instruction, when the first light source or the second light source works, the main menu module sends a spectrum acquisition instruction to the spectrum acquisition module when the cuvette is not added with the solution to be detected or is added with the solution to be detected in two states, the spectrum acquisition module controls the CCD camera of the mobile intelligent terminal to take a picture according to the spectrum acquisition instruction, draws the light intensity into a spectrogram according to the relation between the wavelength and the pixel, sends the spectrogram to the colorimetric module or the fluorescent module according to the detection mode selection, the colorimetric module acquires the light intensities of two specific wavelengths in the state of not adding the solution to be detected or the solution to be detected according to the spectrogram, acquires the absorbance of the solution to be detected at the two specific wavelengths according to a beer's specific formula, acquires the absorbance of the solution to be detected at the two specific wavelengths according to the ratio of the absorbance at the two specific wavelengths and the solution to be detected in the state of not adding the solution to be detected, and acquires the relative concentration of the solution to be detected in the state of the linear state of not changing the relative to the concentration of the solution to be detected in the mobile intelligent terminal.
2. The device of claim 1, wherein the optical sensor attachment further comprises an aperture-sized diaphragm removably disposed between the first focusing lens and the cuvette and coaxial with the first exit path, the aperture being configured to control the amount of light exiting the first optical path after passing through the aperture.
3. The apparatus according to claim 1 or 2, wherein the mobile intelligent terminal further comprises a network sharing module, the concentration display module transmits the concentration to the network sharing module, and the network sharing module distributes the received concentration on a network.
4. The absorption and fluorescence spectrum sensing device according to claim 3, wherein the first light source is a full spectrum LED lamp; the second light source is an LED lamp or a laser lamp with specific wavelength.
5. The apparatus of claim 4, wherein the first focusing lens and the second focusing lens are plano-convex lenses each having a thickness of 1mm, a diameter of 10mm, and a focal length of 25mm and 15mm, respectively; the reflector is a plane reflector, the thickness is 1mm, and the diameter is 10mm.
6. The apparatus of claim 5, wherein the diffraction grating is a transmission grating having a thickness of 2mm and a diameter of 10mm and a line number of not less than 1200lines/mm.
7. The apparatus of claim 6, wherein the first light source and the second light source are connected to a USB interface of the mobile intelligent terminal through a circuit, and the battery of the mobile intelligent terminal supplies power to the first light source and the second light source.
8. The device for detecting absorption and fluorescence spectrum according to claim 7, wherein when the device for detecting absorption and fluorescence spectrum needs to be corrected, a first full-spectrum light source is replaced by laser lights with wavelengths of 405nm, 450nm, 532nm and 650nm in sequence from small to large, after each time the laser lights are turned on, in a state that no solution to be detected is in the cuvette, the main menu module sends a spectrum acquisition instruction to the spectrum acquisition module, the spectrum acquisition module controls the CCD camera to take a picture, records the pixel positions of the emergent light of the current light source in the picture, carries out linear fitting according to the recorded four pixel positions, the spectrum acquisition module obtains the relation between the wavelengths and the pixels, then replaces the light source with the full-spectrum light source, and sends a detection mode selection instruction to the light source control module.
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| CN107917905B (en) * | 2017-12-29 | 2020-11-20 | 山东师范大学 | Ratio type luminosity analysis device based on intelligent terminal and detection method thereof |
| CN109142310A (en) * | 2018-11-13 | 2019-01-04 | 中山大学 | Multivariate detection chip reading instrument based on mobile intelligent terminal |
| CN109632753B (en) * | 2019-01-09 | 2021-07-20 | 山东师范大学 | Portable absorbance measuring device and method thereof |
| CN109975216B (en) * | 2019-03-11 | 2020-07-07 | 浙江大学 | Portable spectrum detection device and method based on laser disc |
| CN110118750A (en) * | 2019-05-13 | 2019-08-13 | 江南大学 | Hand-held spectrophotometer based on smart phone |
| CN110542667A (en) * | 2019-09-29 | 2019-12-06 | 南京同开环保科技有限公司 | Portable rapid water quality detector and water quality detection method |
| WO2023209466A1 (en) * | 2022-04-27 | 2023-11-02 | Madrakian Tayyebeh | Colorimetry using a portable colorimeter |
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