CN106404733B - Food safety detection device and detection method based on mobile intelligent terminal - Google Patents
Food safety detection device and detection method based on mobile intelligent terminal Download PDFInfo
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- CN106404733B CN106404733B CN201610829238.4A CN201610829238A CN106404733B CN 106404733 B CN106404733 B CN 106404733B CN 201610829238 A CN201610829238 A CN 201610829238A CN 106404733 B CN106404733 B CN 106404733B
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- 238000001514 detection method Methods 0.000 title claims abstract description 87
- 230000005284 excitation Effects 0.000 claims abstract description 113
- 238000001914 filtration Methods 0.000 claims abstract description 91
- 238000012360 testing method Methods 0.000 claims abstract description 59
- 230000005540 biological transmission Effects 0.000 claims description 25
- 229910052736 halogen Inorganic materials 0.000 claims description 9
- 150000002367 halogens Chemical class 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 229910052724 xenon Inorganic materials 0.000 claims description 2
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims description 2
- 238000007689 inspection Methods 0.000 claims 6
- 241000276425 Xiphophorus maculatus Species 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 7
- 230000003287 optical effect Effects 0.000 description 7
- 238000012545 processing Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000003908 quality control method Methods 0.000 description 3
- 238000004891 communication Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 239000004005 microsphere Substances 0.000 description 2
- 239000000654 additive Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 125000005843 halogen group Chemical group 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
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- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides a food safety detection device and a detection method based on a mobile intelligent terminal, wherein the detection device comprises a terminal bracket and a detection host, a bracket fixing plug and a terminal image acquisition hole are arranged on the terminal bracket, and the detection host comprises a shell top cover, an image filtering unit, an excitation light unit, a circuit unit, a battery unit, a test strip holder and a shell; the detection device is hand-held, miniaturized, chargeable, offline, compact in structure, convenient to carry, low in equipment manufacturing cost and low in working cost, and the detection method using the detection device can be used for instant detection without being limited by places, is convenient and economic, and improves the working efficiency.
Description
Technical Field
The invention belongs to the technical field of test strip detection, and particularly relates to a food safety detection device and a detection method capable of being matched with terminal equipment such as a mobile smart phone or a tablet.
Background
At present, along with the high importance of people on food safety problems, food safety detection instruments or equipment also become research and development hot spots, and particularly, a simple, convenient, rapid and low-detection-cost immunological detection technology is gradually developed in recent years, the detection of food can be completed on a food safety supervision site by adopting a test strip rapid detection technology, specific reasons causing safety problems in the food can be analyzed, particularly in the detection of toxic food, unsafe factor sources of the food can be rapidly detected, the detection of food illegal additives is facilitated, and the treatment of victims is facilitated.
The test strip for food detection is more and more focused in the detection field by virtue of the advantages of specificity, sensitivity, rapidness and convenience, and can be used for qualitative detection and quantitative or semi-quantitative detection. Colloidal gold test strips and fluorescent test strips should be the most widely used;
when the colloidal gold test strip is used for qualitative detection, the color change of the quality control line and the detection line is visually observed to judge the content of the target detection object; when the quantitative detection is carried out by using a colloidal gold test strip, a special test strip image processing instrument is generally used for collecting the image of the test strip and carrying out a series of processing on the image. Drawing a standard curve or calculating a regression equation according to the ratio of the gray value of the standard substance detection line to the gray value of the quality control line to calculate the concentration of the object to be detected;
the fluorescent test strip takes a fluorescent substance as a marker, the fluorescent substance can emit fluorescence with another wavelength under the irradiation of light with a specific wavelength, and the intensity of the fluorescence represents the concentration of the detection object. When quantitative detection is carried out, a special test strip optical density reader is generally used for scanning the optical density of the detection line and the quality control line, and a standard curve is drawn or a regression equation is calculated according to the optical density value of the standard substance to calculate the concentration of the object to be detected.
Different fluorescent test strips have excitation light and fluorescence with different wavelengths, and common wavelengths are as follows:
| excitation light (nm) | Fluorescence (nm) |
| 200-400 | 545 |
| 475 | 525 |
| 620 | 680 |
| 980 | 545 |
Disclosure of Invention
Therefore, the invention aims to provide a food safety detection device and a detection method based on intelligent electronic equipment so as to realize rapid and accurate detection of food safety.
In order to achieve the above purpose, the technical scheme of the invention is realized as follows:
the food safety detection device based on the mobile intelligent terminal comprises a terminal bracket and a detection host, wherein a bracket fixing plug and a terminal image acquisition hole are arranged on the terminal bracket, and the detection host comprises a shell top cover, an image filtering unit, an excitation light unit, a circuit unit, a battery unit, a test strip holder and a shell;
the shell top cover is provided with a host top cover image acquisition (camera) hole and a bracket fixing jack, the bracket fixing jack is matched with a bracket fixing plug for use, and the size of the host top cover image acquisition hole is equal to that of the terminal image acquisition hole; the image filtering unit is arranged above the excitation light unit, the circuit unit and the battery unit are arranged on two sides below the excitation light unit, a middle gap is a light channel, a test paper holder is further arranged right below the excitation light unit, a hole for taking and placing test paper is formed in the lower part of one side of the shell, and the image filtering unit, the excitation light unit, the circuit unit, the battery unit and the test paper holder are fixed in the shell;
the image filtering unit (because the mobile terminal equipment mainly uses a camera to influence data acquisition, the image rate optical unit can also be called as the camera filtering unit) comprises a first bracket, a first motor, a first driving wheel, a first transmission belt, an image filtering wheel disc and an image filter, wherein the first bracket supporting plate is a horizontal structural plate, a circular image light-passing hole is formed in the first bracket, a plurality of image filtering holes are formed in the image filtering wheel disc, the image filtering holes are provided with image filters which can pass through different wavelengths, the image filtering holes are coaxial with the image light-passing holes of the first bracket, the size of the image filtering holes is equal to that of the image light-passing holes of the first bracket, or the image filtering holes are slightly larger than that of the image filtering holes of the first bracket, the center of the image filtering wheel disc is fixed by an image filtering wheel disc center fixing piece fixedly connected to the first bracket, the first motor is connected with the first driving wheel, the motor is arranged in a first motor mounting hole formed at one end of the first bracket, a first transmission belt is arranged between the first driving wheel and the image wheel, the first motor drives the first transmission belt to drive the first driving wheel and the image wheel to pass through the image filtering wheel to a wide range which can pass through the image filtering device to realize the detection according to the rotation of the detection requirement;
the excitation light unit comprises a second bracket, a second motor, a second driving wheel, a second transmission belt, an excitation light filtering wheel disc, excitation light filters, excitation light and an excitation light bracket, wherein the bottom plate of the second bracket forms 0-45 degrees with the horizontal plane, a special-shaped through hole is formed in the second bracket, the special-shaped through hole is a closed hole formed by two circumscribed circles with different diameters and two public tangential lines, the upper part of the round hole end with the smaller diameter is provided with the excitation light filtering wheel disc, a plurality of excitation light filtering holes are formed in the excitation light filtering wheel disc, the aperture of each excitation light filtering hole is coaxial with the diameter of the small-diameter round hole of the special-shaped through hole, or the aperture of each excitation light filtering hole is smaller than the diameter of the small-diameter round hole of the special-shaped through hole, a rotating shaft is arranged in each excitation light filtering wheel center, the rotating shaft is inserted into the corresponding rotating shaft hole on the second bracket, the excitation light filtering wheel is connected with the second driving wheel through the second transmission belt, the second driving wheel is provided with a second motor to provide power, the second motor is provided with a plate-shaped excitation light filtering hole, the aperture is coaxial with the small-diameter round hole of the special-shaped through hole, the small-diameter round hole is formed in the special-shaped through hole is formed in the excitation light filtering wheel, the excitation light is not fixed on the excitation light bracket, and the excitation light is made into the excitation light emitting material, and is not passes through the hole through the small-shaped hole, and is fixed on the excitation light hole through the hole;
furthermore, the image filtering unit mainly adopts a mechanical structure driven by a belt pulley. The stepping motor (model GM15BY, 20BYGH300 or SY20STH 30-0604) is fixed with the driving wheel to drive the optical filter wheel to rotate; optical filters on the image filter wheel: (1) a short-wave pass filter with a cut-off frequency of 450nm; (2) a bandpass filter having a passband center wavelength of 475nm; (3) a bandpass filter having a passband centered at a wavelength of 620nm; (4) a bandpass filter having a passband centered at 980nm; (5) an infrared cut-off filter having a cut-off wavelength of 800nm;
the working position of the image filter is right opposite to the circular light transmission hole, and the mobile intelligent terminal such as a mobile phone camera and a flat-panel camera is arranged right above the image filter. The image filter is used for filtering light outside the fluorescence wavelength range emitted by the test strip so as to enable the mobile intelligent terminal, such as a mobile phone camera and a flat-panel camera, to receive useful signals and eliminate interference of light with other wavelengths;
further, the excitation light unit: the device mainly adopts a mechanical structure driven by a belt pulley, and a stepping motor is fixed with a driving wheel to drive an excitation light filter wheel to rotate; excitation light filters on the excitation light filter wheel: (1) a bandpass filter having a passband centered at a wavelength of 525nm; (2) a bandpass filter having a passband center wavelength of 545nm; (3) a bandpass filter having a passband center wavelength of 680nm; (4) an infrared cut-off filter having a cut-off wavelength of 800nm; (5) reservation; the excitation light mainly adopts a halogen lamp, the halogen lamp is a broad-spectrum light source right above the excitation light filter, and the emission wavelength range of the halogen lamp can reach: 300nm-3600nm, and the excitation light wavelength of the common fluorescent test strip is covered. The excitation light filter is used for filtering light outside the wavelength range of the excitation light (emitted by the halogen lamp) so as to reduce interference of the wavelength light; the aperture of the special-shaped light-passing hole of the excitation light unit is larger, so that excitation light emitted by the halogen lamp can pass through, and the camera can acquire an image of the test strip;
further, the circuit unit: mainly comprises: bluetooth communication module (HC-05, HC-06 or cc 2541), CPU (model M2378-CFS201 chip, or model LPC2366 chip), motor driving module (A4980 or THB 7128);
further, the distance between the host top cover image acquisition (camera) hole and the test strip holder is in the range: 20-180mm;
further, the excitation light in the excitation light unit can be selected from a halogen lamp, a xenon lamp or an LED lamp group;
further, the belt pulley transmission devices in the image filtering unit and the excitation light unit can be replaced by gears, screw rods, worm gears or motors for direct driving;
further, the outer shell is black inside and is made of light-proof or light-proof materials.
The food safety detection method of the mobile intelligent terminal comprises the following steps:
s1, placing mobile terminal equipment with a detection application system on a terminal bracket, aligning a camera or an image acquisition device of the mobile terminal with a terminal image acquisition hole, and placing the terminal equipment and the terminal bracket on a detection host;
s2, inserting a test strip to be detected into a test strip holder;
s3, connecting the terminal equipment and the detection host through the connection module, operating the control application of the terminal equipment, selecting a detection mode of a corresponding test strip, and driving the first motor and the second motor to act after the detection host obtains a signal; the excitation light filter wheel disc is rotated to the position of the required excitation light filter and the coaxial upper part of the small-diameter round hole of the special-shaped light transmission hole, and the image filter wheel disc is rotated to the position of the required image filter and the coaxial upper part of the image light transmission hole;
s4, according to the type of the test paper to be detected, under the control of the circuit unit, the excitation light is turned on or off according to the requirement;
s5, operating a detection application module of the terminal equipment, and collecting information on the test paper strip to be detected; meanwhile, an analysis module on the terminal equipment analyzes and processes the acquired information, and displays and outputs the result of the object to be detected.
Compared with the prior art, the food safety detection device based on the mobile intelligent terminal has the following advantages:
the detection device is an instrument capable of quantitatively detecting the colloidal gold test strip and the fluorescent test strip, namely the detection device can finish quantitative detection by calling a camera through a matched mobile intelligent terminal, such as a mobile phone application system (APP);
the detection device is hand-held, miniaturized, chargeable and offline, and has the advantages of exquisite structure, portability and lower equipment manufacturing cost;
the detection device can be suitable for mobile phones (with camera and Bluetooth functions) of any type of intelligent terminals, and can adapt to all appearance intelligent terminal equipment by changing the modeling of a mobile phone bracket and the position of a fixed plug on the bracket;
the food safety detection method provided by the invention reduces the working cost to a great extent, can realize the requirement of instant detection because of being not limited by places, is convenient and economic, and improves the working efficiency.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention and do not constitute an undue limitation on the invention. In the drawings:
FIG. 1 is a schematic view of a detection device according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of a mobile intelligent terminal bracket according to an embodiment of the present invention;
FIG. 3 is a schematic view of a housing of a detection device according to an embodiment of the present invention;
FIG. 4 is a cross-sectional view of a detection device according to an embodiment of the present invention;
FIG. 5 is an assembly view of a detection device according to an embodiment of the present invention;
FIG. 6 is a schematic diagram of an image filtering unit in the detecting device according to an embodiment of the invention;
FIG. 7 is a schematic view of a light source unit in a detection device according to an embodiment of the present invention;
fig. 8 is a schematic diagram of a detection flow according to an embodiment of the invention.
Fig. 9 is a schematic diagram of a circuit unit module.
Reference numerals illustrate:
1-a terminal bracket; 2-detecting a host; 10-terminal image acquisition holes; 11-bracket fixing plug; 200-a host top cover image acquisition hole; 201-bracket fixing insertion holes; 20-a housing top cover; 21-an image filtering unit; 22-an excitation light unit; 23-a circuit unit; 24-battery cells; 25-test strip holders; 26-a housing; 210-a first bracket; 2100-image light-passing aperture; 2101-a first motor mounting hole; 211-a first motor; 212-a first driving wheel; 213-a first drive belt; 214-an image filter wheel; 215-an image filter; 216-an image filter wheel center fixing piece; 220-a second bracket; 221-a second motor; 222-a second drive wheel; 223-a second drive belt; 224—excitation light filter wheel; 225-an excitation light filter; 226-excitation light; 227-an excitation light bracket; 2201-special-shaped light passing holes; 2202-motor mounting holes.
Detailed Description
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.
In the description of the invention, it should be understood that the terms "center," "longitudinal," "transverse," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships that are based on the orientation or positional relationships shown in the drawings, merely to facilitate describing the invention and simplify the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be configured and operate in a particular orientation, and therefore should not be construed as limiting the invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the creation of the present invention can be understood by those of ordinary skill in the art in a specific case.
The invention will be described in detail below with reference to the drawings in connection with embodiments.
As shown in fig. 1-5, a food safety detection device based on a mobile intelligent terminal comprises a terminal bracket 1 and a detection host 2, wherein a bracket fixing plug 11 and a terminal image acquisition hole 10 are formed in the terminal bracket 1, the detection host 2 comprises a shell top cover 20, an image filtering unit 21, an excitation light unit 22, a circuit unit 23, a battery unit 24, a test paper strip holder 25 and a shell 26, a host top cover image acquisition hole 200 and a bracket fixing jack 201 are formed in the shell top cover 20, the bracket fixing jack 201 is matched with the bracket fixing plug 11 for use, and the host top cover image acquisition hole 200 is coaxial with the terminal image acquisition hole 10; the size of the host top cover image acquisition hole 200 is equal to that of the terminal image acquisition hole 10; the image filtering unit 21 is arranged above the excitation light unit 22, the circuit unit 23 and the battery unit 24 are arranged on two sides below the excitation light unit 22, a middle gap is a light channel, a test strip holder 25 is further arranged right below the excitation light unit 22, a hole for taking and placing test paper is formed in the lower part of one side of the shell 26, and the image filtering unit 21, the excitation light unit 22, the circuit unit 23, the battery unit 24 and the test strip holder 25 are fixed in the shell 26; the circuit unit 23 and the battery unit 24 are electrically connected with the image filtering unit 21 and the excitation light unit 22;
as shown in fig. 6, the image filtering unit 21 includes a first bracket 210, a first motor 211, a first driving wheel 212, a first driving belt 213, an image filtering wheel 214 and an image filter 215, where the supporting plate of the first bracket 210 is a horizontal structural plate, a circular image light-passing hole 2100 is formed in the first bracket 210, a plurality of image filtering holes are formed in the image filtering wheel 214, image filters 215 capable of passing through different wavelengths are formed in the image filtering holes, the image filtering holes are coaxial with the image light-passing holes 2100 of the first bracket 210 and have the same size, the center of the image filtering wheel 214 is fixed by an image filtering wheel center fixing member 216 fixedly connected to the first bracket 210, the first motor 211 is connected with the first driving wheel 212, the motor 211 is disposed in a first motor mounting hole 2101 formed at one end of the first bracket 210, a first driving belt 213 is disposed between the first driving wheel and the image filtering wheel 214, the first motor 211 drives the first driving wheel 212, the first driving wheel 212 drives the image filtering wheel 214 to rotate through the first driving belt 213, and the image filtering wheel 215 rotates to a different working range according to the image detection requirement of the image light-passing device 2100;
as shown in fig. 7, the excitation light unit 22 includes a second bracket 220, a second motor 221, a second driving wheel 222, a second driving belt 223, an excitation light filtering wheel 224, an excitation light filter 225, excitation light 226 and an excitation light bracket 227, the bottom plate of the second bracket 220 forms 0 ° -45 ° with the horizontal plane, a special-shaped through hole 2201 is formed in the second bracket 220, the special-shaped through hole 2201 is a closed hole formed by two circumscribed circles with different diameters and two common tangential lines, the upper part of the round hole end with a smaller diameter is provided with an excitation light filtering wheel 224, the excitation light filtering wheel 224 is provided with a plurality of excitation light filtering holes corresponding to the small diameter round hole of the special-shaped through hole 2201, each excitation light filtering hole is provided with an excitation light filter 225, a rotating shaft is arranged in the center of the excitation light filtering wheel 224, the rotating shaft is inserted into the corresponding rotating shaft hole in the second bracket 220, the special-shaped through hole 2201 is connected with the second driving wheel 222 through the second driving belt 223, the second driving wheel 222 is provided with a power transmission wheel 226, the second driving wheel 221 is further provided with a light transmission hole for preventing the excitation light from being emitted from being formed on the second bracket 220, and the special-shaped through hole is formed in the small diameter of the excitation light filtering wheel 2201, and the excitation light filter is formed on the second bracket 220 is not to be provided with a light transmission hole.
As shown in fig. 8, the detection method of the food safety detection device using the mobile intelligent terminal comprises the following steps: the mobile intelligent terminal takes a mobile phone as an example, the connection module takes a Bluetooth module as an example, the image acquisition module takes a camera as an example, the flow of the detection device is described in detail, and the switching of different optical filters is realized through the first motor, the second motor, the first driving belt and the second driving belt so as to detect fluorescent test strips and colloidal gold test strips of different excitation lights and emitted lights.
Detecting a colloidal gold test strip: the test strip is inserted into the test strip holder, a control application part (APP) of the mobile phone is operated, the mobile phone is driven by the first motor and the second motor through the Bluetooth control system, the light filters of the excitation light unit and the image filtering unit are all turned to an infrared cut-off light filter, the colloidal gold test strip does not need excitation light, an excitation light source does not need to be turned on, and the excitation light is turned on to illuminate in order to improve the brightness; and operating a detection application system on the mobile phone to take a picture, and performing digital image processing on the picture by the mobile phone detection system to convert the concentration of the object to be detected on the test strip.
And (3) fluorescent paper strip detection: the test strip is inserted into the test strip holder, a control application part of the mobile phone is operated, the light filters passing through the Bluetooth control system, the excitation light source unit and the image filtering unit are all turned to the light filters with corresponding wavelengths, the light source is turned on, a detection application system on the mobile phone is operated to take a picture, the mobile phone application detection system carries out digital image processing on the picture, and the concentration of an object to be detected on the test strip is converted.
Specific examples are: detecting a test strip taking fluorescent microspheres as a marker, irradiating excitation light with a wavelength of 475nm to emit 545nm fluorescence, wherein a light filter of an excitation light unit is provided with a light which can penetrate the wavelength of 475nm, the excitation light unit is a halogen lamp, and an image light filter is provided with a light which can penetrate the wavelength of 545nm;
s1, placing a mobile phone into a mobile phone bracket and installing the mobile phone on a detection instrument;
s2, inserting the test strip into a test strip holder;
s3, operating the APP, and selecting a detection mode of a corresponding fluorescent microsphere test strip; at the moment, the mobile phone can communicate with the instrument through Bluetooth, and the circuit unit of the instrument can drive the stepping motor to act after obtaining signals; rotating the filter wheel of the excitation light unit to a 475nm filter position, and rotating the filter wheel of the image filter unit to a 545nm filter position;
s4, under the control of the circuit unit, the halogen lamp is turned on;
s5, shooting a photo through an APP operation, and collecting test strip images;
s6, performing digital image processing on the photo by the mobile phone APP, and finally converting the concentration of the to-be-detected object on the test strip.
The above embodiments are merely preferred embodiments of the present invention and are not intended to limit the present invention, and any modifications, equivalent substitutions, improvements, etc. within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (8)
1. A food safety detection device, characterized in that: comprises a terminal bracket (1) and a detection host (2);
the terminal bracket (1) is provided with a bracket fixing plug (11) and a terminal image acquisition hole (10), the detecting host (2) is provided with a shell top cover (20), the shell top cover (20) is provided with a host top cover image acquisition hole (200) and a bracket fixing jack (201), the bracket fixing jack (201) is assembled with the bracket fixing plug (11) in a matched mode, and the host top cover image acquisition hole (200) and the terminal image acquisition hole (10) are equal in size and coaxial;
the detection host (2) further comprises an image filtering unit (21), an excitation light unit (22), a circuit unit (23), a battery unit (24), a test strip holder (25) and a shell (26); the image light filtering unit (21) is arranged above the excitation light unit (22), the excitation light unit (22) is arranged above the test paper strip holder (25) and is positioned inside the shell (26), the image light filtering unit (21) and the excitation light unit (22) are provided with corresponding light filters, the circuit unit (23) and the battery unit (24) are also arranged inside the shell (26) and are electrically connected with the image light filtering unit (21) and the excitation light unit (22), and a light beam channel is formed between the circuit unit (23) and the battery unit (24);
the excitation light unit (22) can emit light, and the light enters a light beam channel through a light filter of the excitation light unit (22) to irradiate a test strip to be detected on the test strip holder (25), and the light is reflected and emitted through the light filter of the image light filtering unit (21);
the image filtering unit (21) comprises a first bracket (210), a first motor (211), a first driving wheel (212), a first transmission belt (213), an image filtering wheel disc (214) and an image filtering sheet (215), wherein the first bracket (210) is a horizontal structure plate, a circular image light transmission hole (2100) is formed in the first bracket (210), a plurality of image filtering holes are formed in the image filtering wheel disc (214), the image filtering sheet (215) is arranged on the image filtering holes, the image filtering holes are coaxial with the image light transmission holes (2100) of the first bracket (210) and are equivalent in size, the center of the image filtering wheel disc (214) is fixed through an image filtering wheel disc center fixing piece (216) fixedly connected to the first bracket (210), the first motor (211) is connected with the first driving wheel (212), the motor (211) is arranged in a first motor mounting hole (2101) formed in one end of the first bracket (210), a first transmission belt (213) is arranged between the first driving wheel and the image filtering wheel disc (214), and the first driving wheel (212) is driven by the first driving wheel (212) to rotate;
the excitation light unit (22) comprises a second bracket (220), a second motor (221), a second driving wheel (222), a second transmission belt (223), an excitation light filtering wheel disc (224), an excitation light filter (225), excitation light (226) and an excitation light bracket (227), wherein a special-shaped light passing hole (2201) is formed in the second bracket (220), the special-shaped light passing hole (2201) is a closed hole formed by two circumscribed circles with different diameters and two common tangents, the upper part of the round hole end with smaller diameter is provided with the excitation light filtering wheel disc (224), the upper end of the excitation light filtering wheel (2201) is provided with the excitation light filtering wheel disc (224), the excitation light filtering wheel disc (224) is provided with a plurality of excitation light filtering holes, each excitation light hole is provided with one excitation light filter (225), the center of the excitation light filtering wheel (224) is provided with a rotating shaft, the rotating shaft is inserted into the rotating shaft hole corresponding to the second bracket (220), the excitation light filtering motor (224) is provided with the second transmission belt (220) through the second driving wheel (220) and is also provided with the second transmission belt (220) which is connected with the second driving wheel (220), excitation light (226) is arranged above the excitation light filtering wheel disc (224), and the excitation light (226) is fixed on an excitation light bracket (227).
2. The food safety inspection device according to claim 1, wherein: the transmission belt mode of the first driving wheel (212) and the image filtering wheel disc (214) in the image filtering unit (21) is replaced by the transmission mode of gear engagement of the first driving wheel (212) and the image filtering wheel disc (214); or the image filtering wheel disc (214) is directly connected with the output end of the first motor (211); or the image filter wheel disc (214) is of a worm wheel structure, the output end of the first motor (211) is of a worm structure, and the image filter wheel disc is driven in a worm and gear mode.
3. The food safety inspection device according to claim 1, wherein: the transmission belt mode of the second driving wheel (222) and the excitation light filtering wheel disc (224) in the excitation light unit (22) is replaced by the transmission mode of gear engagement of the second driving wheel (222) and the excitation light filtering wheel disc (224); or the excitation light filtering wheel disc (224) is directly connected with the output end of the second motor (221); or the excitation light filtering wheel disc (224) is of a worm gear structure, and the output end of the second motor (221) is of a worm structure and is driven in a worm and gear mode.
4. The food safety inspection device according to claim 1, wherein: the distance between the host top cover image acquisition hole (200) and the test strip holder (25) is 20-180mm.
5. The food safety inspection device according to claim 1, wherein: the excitation light bracket (227) is made of a bent platy light-proof material; the housing (26) is internally black and opaque, or is made of an opaque material.
6. The food safety inspection device according to claim 1, wherein: the bottom plate of the second bracket (220) is 0-45 degrees with the horizontal plane.
7. The food safety inspection device according to claim 1, wherein: the excitation light (226) in the excitation light unit (22) is a halogen lamp or a xenon lamp or an LED lamp group.
8. A food safety detection method based on a mobile intelligent terminal is characterized in that: the method comprises the following steps:
s1, placing mobile terminal equipment with a detection application system on a terminal bracket (1), aligning a camera or an image acquisition device of the mobile terminal with a terminal image acquisition hole (10), and placing the terminal equipment and the terminal bracket (1) on a detection host (2);
s2, inserting a test strip to be detected into a test strip holder (25);
s3, connecting the terminal equipment with the detection host (2) through the connection module, operating the control application of the terminal equipment, selecting a detection mode of a corresponding test strip, and driving the first motor (211) and the second motor (221) to act after the detection host (2) obtains a signal; the excitation light filter wheel disc (224) is rotated to the position of a required excitation light filter (225) and the coaxial upper part of the small-diameter round hole of the special-shaped light transmission hole (2201), and the image filter wheel disc (214) is rotated to the position of a required image filter (215) and the coaxial upper part of the image light transmission hole (2100);
s4, according to the type of test paper to be detected, under the control of a circuit unit (23), the excitation light (226) is turned on or off according to the requirement;
s5, operating a detection application module of the terminal equipment, and collecting information on the test paper strip to be detected; meanwhile, an analysis module on the terminal equipment analyzes and processes the acquired information, and displays and outputs the result of the object to be detected.
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| CN108627482A (en) * | 2017-03-20 | 2018-10-09 | 上海溯源生物技术有限公司 | Fluorescence detection device and its fluorimetric analysis method based on mobile terminal |
| CN107255712B (en) * | 2017-05-03 | 2019-08-06 | 上海交通大学 | A kind of immunochromatography chip quantified system analysis based on smart phone |
| CN108008126B (en) * | 2017-12-30 | 2023-10-27 | 天津博硕东创科技发展有限公司 | Test paper strip ejecting mechanism |
| CN109342416B (en) * | 2018-11-30 | 2023-04-21 | 李思源 | Noninvasive spectrum detection device based on smart phone and application |
| CN116338159B (en) * | 2022-12-13 | 2024-02-09 | 西交利物浦大学 | Full-automatic paper-based micro-fluidic system based on smart phone local detection |
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