CN108731805A - Absorption based on mobile intelligent terminal and fluorescence spectrum detecting device - Google Patents
Absorption based on mobile intelligent terminal and fluorescence spectrum detecting device Download PDFInfo
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- CN108731805A CN108731805A CN201710245312.2A CN201710245312A CN108731805A CN 108731805 A CN108731805 A CN 108731805A CN 201710245312 A CN201710245312 A CN 201710245312A CN 108731805 A CN108731805 A CN 108731805A
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- 238000000862 absorption spectrum Methods 0.000 claims abstract description 12
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/0205—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows
- G01J3/0208—Optical elements not provided otherwise, e.g. optical manifolds, diffusers, windows using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/42—Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry
- G01J3/427—Dual wavelengths spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/314—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry with comparison of measurements at specific and non-specific wavelengths
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/02—Details
- G01J3/10—Arrangements of light sources specially adapted for spectrometry or colorimetry
- G01J2003/102—Plural sources
- G01J2003/106—Plural sources the two sources being alternating or selectable, e.g. in two ranges or line:continuum
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N2021/6463—Optics
- G01N2021/6467—Axial flow and illumination
Abstract
The invention discloses a kind of absorption based on mobile intelligent terminal and fluorescence spectrum detecting devices, including mobile intelligent terminal, optical sensing attachment and adapter, optical sensing attachment is installed on by adapter on mobile intelligent terminal, when selecting colorimetric detection pattern, lamp source one is opened, when selecting fluoroscopic examination pattern, lamp source two is opened, the light through cuvette along emitting light path one reflects on the mirror, reflected light is according on the diffraction grating, it is acted on by the light splitting of diffraction grating, forms a band, reach CCD camera；Spectra collection module, colorimetric module, fluorescent moieties in mobile intelligent terminal can finally obtain the concentration of solution to be measured according to the photo that CCD camera is shot, this absorption and fluorescence spectrum detecting device light structure are portable, it is easy to operate and cheap, using two kinds of detection patterns of colorimetric and fluorescence, it can realize that the carry out real-time on-site to determinand, high-precision multi-mode fast spectrum detect.
Description
Technical field
The present invention relates to spectrometer technical fields, more particularly to the absorption based on mobile intelligent terminal and fluorescence spectrum detection
Device.
Background technology
With the development of computer technology, mobile intelligent terminal (such as smart mobile phone, tablet computer etc.) gradually at
Indispensable tool in for people's lives.Due to CPU with high performance so that mobile intelligent terminal has computer
The function being had, by adding some simple attachmentes, the function of general large-scale instrument can be realized in mobile intelligent terminal.
Demand with people to detecting in real time, spectral detection is in food inspection, environmental monitoring, medical diagnosis on disease and clinical prison
The fields such as shield have a wide range of applications and specific industrialization prospect.Conventional spectrum detection technique due to system bulk heaviness,
The deficiencies of expensive and complicated for operation so that these technologies cannot be directly used to field quick detection.It is directed to this deficiency,
Some miniature light are developed with U.S.'s marine optics, Holland Ai Wantisi, Japan shore the Song Dengwei company represented or team
Spectrometer.Although these equipment can accomplish hand size, for price less than ten thousand yuan, they are still the hard of independent completion
Part system, including light source, circuit, outer aobvious equipment etc. can not achieve really portable.Fortunately, the height of mobile intelligent terminal
Resolution ratio camera can shoot visible light, and terminal screen can be used as display equipment, it is only necessary to design light source relevant mode
Block is combined with mobile intelligent terminal, then is aided with terminal software, you can realizes the spectral detection dress based on mobile intelligent terminal platform
It sets.Compared with currently a popular micro spectrometer, this kind of spectrum detection device has lower cost and better portability.Separately
Outside, by mobile Internet, this kind of spectrum detection device can also directly upload data, be not necessarily to the additional network equipment.
But the current spectrum detection device based on mobile intelligent terminal can only often realize single detection pattern,
Such as can only realize colorimetric detection or fluoroscopic examination, that there are sensitivity is low for single detection pattern, detection range is small or specific
The deficiencies of poor, limits the application of this kind of spectrum detection device quick context of detection at the scene.A variety of detection patterns detect simultaneously,
Testing result can mutually be corrected, it is ensured that therefore the accuracy of testing result realizes real-time, multi-mode inspection at the scene
It is urgently to be resolved hurrily to survey this demand.
Invention content
The present invention provides a kind of absorption based on mobile intelligent terminal and fluorescence spectrum detecting device, can carry out in real time
On the spot, high-precision multi-mode fast spectrum detects.
A kind of absorption and fluorescence spectrum detecting device based on mobile intelligent terminal, including：With CCD camera, touch-control
The optical sensing attachment is installed on mobile intelligence by the mobile intelligent terminal of display screen and central processing unit, optical sensing attachment
Adapter in energy terminal；The optical sensing attachment includes for the lamp source one of colorimetric detection pattern and for fluoroscopic examination mould
The lamp source two of formula, the emitting light path one of lamp source one are mutually perpendicular to the emitting light path two of lamp source two；The optical sensing attachment is also
Including cuvette, the cuvette and the emitting light path one and the emitting light path two are coaxial, and the lamp source one is full spectrum
The emergent light of light source, the lamp source two excites the solution in the cuvette to generate the fluorescence for detecting required wavelength, the optics
Sensing attachment further include in the cuvette rear end and the speculum that be arranged along the emitting light path one, the emitting light path one and
The speculum there are incidence angle, the optical sensing attachment further include setting the CCD camera and the speculum it
Between diffraction grating, through cuvette and along the emergent light of the emitting light path one by the speculum reflection after vertical incidence institute
Diffraction grating is stated, for diffraction on CCD camera, the optical sensing attachment further includes lamp source control again through the diffraction grating
Module, the lamp source control module are connect with each lamp source circuit, control the opening and closing of each lamp source respectively；
The mobile intelligent terminal includes that main menu block, spectra collection module, colorimetric module, fluorescent moieties, concentration are aobvious
Show module；The main menu block sends selection detection pattern instruction, the lamp source control module to the lamp source control module
The lamp source one or the lamp source two is selected to work according to selection detection pattern instruction, when the lamp source one or the lamp source
When two work, in the case where the cuvette is not added solution to be measured or solution two states to be measured is added, the main menu block
Spectra collection instruction is sent to the spectra collection module, the spectra collection module instructs control institute according to the spectra collection
The CCD camera for stating mobile intelligent terminal takes pictures, obtain the luminous intensity of each pixel in photo, according to wavelength and pixel it
Between relationship the luminous intensity is depicted as spectrogram, according to selection detection pattern to the colorimetric module or the fluorescent moieties
The spectrogram is sent, the colorimetric module obtains according to the spectrogram and solution to be measured or addition solution to be measured is added no
It is specific at two to obtain solution to be measured further according to langbobier law formula for the luminous intensity of two specific wavelengths under two states
Absorbance at wavelength, according to the linear relationship between the ratio and testing concentration of the absorbance of described two certain wave strong points
The concentration of determinand is obtained, the fluorescent moieties obtain a certain certain wave strong point luminous intensity in the spectrogram and waited in no addition
The relative changing value for surveying solution or being added under solution two states to be measured, according to the relative changing value and solution concentration to be measured it
Between linear relationship obtain the concentration of solution to be measured, the concentration display module receives the colorimetric module or the fluorescence mould
The concentration that block is sent, shows the concentration on the touching display screen of the mobile intelligent terminal.
In the present invention, optical sensing attachment is installed on mobile intelligent terminal by adapter, and user can select colorimetric
With two kinds of detection patterns of fluorescence, when selecting colorimetric detection pattern, lamp source one is opened, when selecting fluoroscopic examination pattern, lamp source
Two open, the emergent light of lamp source two can excite the solution in cuvette to generate fluorescence, along emitting light path one through cuvette
Light reflects on the mirror, and reflected light acts on by the light splitting of diffraction grating according on the diffraction grating, forms a spectrum
Band reaches CCD camera；Spectra collection module, colorimetric module in mobile intelligent terminal, fluorescent moieties can be taken the photograph according to CCD
As the photo of head shooting finally obtains the concentration of solution to be measured, it can be seen that, absorption that should be based on mobile intelligent terminal and fluorescence
Spectrum detection device light structure is portable, easy to operate and cheap, utilizes two kinds of detection patterns of colorimetric and fluorescence, Neng Goushi
Now the carry out real-time on-site of determinand, high-precision multi-mode fast spectrum are detected.
Description of the drawings
Fig. 1 is the knot of a kind of absorption and fluorescence spectrum detecting device based on mobile intelligent terminal in the embodiment of the present invention
Structure schematic diagram；
Fig. 2 is a kind of principle of absorption and fluorescence spectrum detecting device based on mobile intelligent terminal in the embodiment of the present invention
Schematic diagram；
Fig. 3 is a kind of wavelength of absorption and fluorescence spectrum detecting device based on mobile intelligent terminal in the embodiment of the present invention
Correcting process figure between pixel；
Fig. 4 is a kind of detection of absorption and fluorescence spectrum detecting device based on mobile intelligent terminal in the embodiment of the present invention
Flow chart.
Specific implementation mode
The embodiment of the present invention provides a kind of absorption based on mobile intelligent terminal and fluorescence spectrum detecting device, carries out below
It is described in detail.
In the present embodiment, mobile intelligent terminal is smart mobile phone, solution to be measured is nanogold reaction solution.
Referring to Fig.1, the absorption based on mobile intelligent terminal and fluorescence spectrum detecting device include smart mobile phone 01, adapter
02 and optical sensing attachment 03, optical sensing attachment 03 be fixed on smart mobile phone 01 by adapter 02.
With reference to Fig. 2, smart mobile phone 01 has CCD camera 9, display screen 10 and central processing unit, optical sensing attachment 03
Including the lamp source 1 for colorimetric detection pattern and the lamp source 22 for fluoroscopic examination pattern, the emitting light path one of lamp source 1
It is mutually perpendicular to the emitting light path two of lamp source 22；Optical sensing attachment 03 further includes cuvette 3, cuvette 3 and emitting light path one
Coaxial with emitting light path two, the emergent light of lamp source 1 transmits cuvette 3, and lamp source 1 is full spectrum light source, lamp source 22
Emergent light excites the solution in cuvette 3 to generate the fluorescence for detecting required wavelength, and fluorescence can also be transmitted along one direction of emitting light path
Go out cuvette 3, optical sensing attachment 03 further includes in 3 rear end of cuvette and the speculum 6 that is arranged along emitting light path one, emergent light
For Lu Yiyu speculums 6 there are incidence angle, optical sensing attachment 03 further includes spreading out of being arranged between CCD camera 9 and speculum 6
Penetrate grating 8, through cuvette 3 and along the emergent light of emitting light path one by speculum 6 reflection after vertical incidence diffraction grating 8, thoroughly
Crossing diffraction grating 8, for diffraction on CCD camera 9, optical sensing attachment 03 further includes lamp source control module again, the lamp source control
Module is connect with lamp source 1 and 2 circuit of lamp source, controls the opening and closing of each lamp source respectively.
It should be noted that can be that lamp source 1 is sent out through cuvette 3 and along the emergent light of emitting light path one herein
The light gone out can also be the fluorescence that lamp source 22 excites.
It should also be noted that, the emitting light path two of the emitting light path one of lamp source 1 and lamp source 22 be mutually perpendicular to be because
The fluorescence signal relative excitation light of solution to be measured is weaker, in order to reduce the interference of exciting light.
It should also be noted that, the effect of diffraction grating 8 is, when lamp source is full spectrum light source, diffraction grating 8 is utilized
The reflected light of speculum 6 is divided.
In the present embodiment, optionally, optical sensing attachment 03 further includes aperture 4, the condenser lens of adjustable aperture size
One 5 and condenser lens 27, since the sensitivity of detection device can be influenced very much by force through the light of cuvette, in order to improve detection dress
The sensitivity set, aperture 4 are used for controlling the light into mobile phone CCD camera 9, and condenser lens 1 is to reduce lamp source 1
Waste of the emergent light on speculum 6 can focus on the surface of speculum 6 by focusing through the light of aperture 4, gather
Focus lens 27 are influenced by incident light shape due to grating diffration quality, for making the reflection luminous energy of speculum 6 focus on
The surface of diffraction grating 8, specially：Pluggable aperture 4 and pluggable is disposed between cuvette 3 and speculum 6
Condenser lens 1, reflected by speculum 6 through the light of condenser lens 1, reflected light penetrates 27 He of condenser lens
Diffraction grating 8 reaches the CCD camera 9 of smart mobile phone 01.
In the present embodiment, optionally, lamp source 1 is full-spectrum LED lamp, and lamp source 22 is the LED light of specific wavelength or swashs
Light lamp.
In the present embodiment, optionally, condenser lens 1 and condenser lens 27 are planoconvex spotlight, and thickness is 1mm, directly
Diameter is 10mm, and focal length is respectively 25mm and 15mm；Speculum 6 is plane mirror, thickness 1mm, a diameter of 10mm；Diffraction
Grating 8 is transmission grating, and thickness 2mm, a diameter of 10mm, groove is not less than 1200lines/mm.
In the present embodiment, optionally, cuvette 3 is cleaned for convenience, and optical sensing attachment 03 further includes a sample
Slot, cuvette 3 are fastened in sample cell, can be flexibly removed or placed into.
Optical sensing attachment 03 further includes lamp source control module, and lamp source control module is electric with lamp source 1 and lamp source 22 respectively
Road is connected.Optionally, lamp source 1 and lamp source 22 are also connect with the usb circuit of mobile phone, by the internal battery of mobile phone to lamp
Source 1 and lamp source 22 are powered.
Smart mobile phone include main menu block, spectra collection module, colorimetric module, fluorescent moieties, concentration display module and
Network share module.
Main menu block sends selection detection pattern instruction to lamp source control module, and lamp source control module is detected according to selection
Mode instruction controls lamp source 1 or lamp source 22 works, when lamp source 1 or the work of lamp source 22, in cuvette 3 that nanometer is not added
Under golden reaction solution or addition nanogold reaction solution two states, in other words,：Under colorimetric detection pattern, one 1 work of lamp source
Make, is examined in the case where cuvette 3 is without being added nanogold reaction solution or nanogold reaction solution two states being added, or in fluorescence
Under survey pattern, lamp source 22 works, in cuvette 3 that nanogold reaction solution is not added or two kinds of nanogold reaction solution is added
Under state, main menu block sends spectra collection instruction to spectra collection module, and spectra collection module is instructed according to spectra collection
The CCD camera 9 of control smart mobile phone is taken pictures, and the luminous intensity of each pixel in photo is obtained, according between wavelength and pixel
Luminous intensity is depicted as spectrogram by relationship, and spectrogram is sent to colorimetric module or fluorescent moieties according to detection pattern.
When selecting detection pattern for than color pattern, colorimetric module is obtained according to spectrogram reacts in no addition nanogold
The luminous intensity of solution or two specific wavelengths being added under nanogold reaction solution two states, that is, colorimetric module is according to spectrum
Figure obtains the 520nm wavelength and 625nm that nanogold reaction solution is not added or is added under nanogold reaction solution two states
Luminous intensity at wavelength, further according to langbobier law formula：Obtain the suction of the nanogold reaction solution corresponding to 625nm wavelength
Luminosity A625With the absorbance A of the nanogold reaction solution corresponding to 520nm wavelength520, and then obtain the ratio A of absorbance625/
A520, due within the scope of a certain concentration, ratio A625/A520Linearly increase with the increase of testing concentration, according to ratio with
Linear relationship between testing concentration obtains the concentration of determinand.
When select detection pattern for fluorescence mode when, fluorescent moieties obtain in spectrogram that luminous intensity is not having at 517nm wavelength
There is the relative changing value that solution to be measured is added or is added under solution two states to be measured, due within the scope of a certain concentration, relatively
Changing value linearly increases with the increase of testing concentration, according to the linear relationship between relative changing value and testing concentration
Obtain the concentration of determinand.
The concentration that concentration display module is sended over according to colorimetric module or fluorescent moieties is shown on mobile phone display screen 10
Concentration information, and corresponding concentration is sent to network share module, network share module receives concentration and is published on network.
In the present embodiment, optical sensing attachment is installed on smart mobile phone by adapter, user can select colorimetric and
Two kinds of detection patterns of fluorescence, when selecting colorimetric detection pattern, lamp source one is opened, when selecting fluoroscopic examination pattern, lamp source two
It opens, the emergent light of lamp source two can excite the solution in cuvette to generate fluorescence, and the light and lamp source 22 that lamp source 1 emits excite
The fluorescence that solution generates reaches after cuvette 3, aperture 4, condenser lens 5, speculum 6, condenser lens 7 and diffraction grating 8
CCD camera 9, what spectra collection module, colorimetric module, fluorescent moieties in smart mobile phone can be shot according to CCD camera
Photo finally obtains the concentration of solution to be measured, it can be seen that, this absorption and fluorescence spectrum detection dress based on mobile intelligent terminal
It is portable to set light structure, it is easy to operate and cheap, using two kinds of detection patterns of colorimetric and fluorescence, can realize to determinand
Carry out real-time on-site, high-precision multi-mode fast spectrum detection.
With reference to Fig. 3, absorption and fluorescence spectrum detecting device in the present embodiment also have calibration function, can examined
Before survey, the relationship between tuning wavelength and photograph pixel will be detailed below correction course with reference to Fig. 3.
In the state that the emergent light of 301. recording wavelength 405nm laser lamps does not have a nanogold reaction solution in cuvette
Location of pixels P in photo405；
Lamp source one is replaced with into the laser lamp that wavelength is 405nm by full spectrum lamp source, lamp source control module is according in mobile phone
The selection detection pattern instruction that main menu block is sent controls laser lamp work, does not have nanogold reaction solution in cuvette
In the state of, spectra collection module of the main menu block into mobile phone sends spectra collection instruction, and spectra collection module controls hand
The CCD camera of machine is taken pictures, and location of pixels P of the emergent light of the laser lamp in photo is recorded405。
In the state that the emergent light of 302. recording wavelength 450nm laser lamps does not have a nanogold reaction solution in cuvette
Location of pixels P in photo450；
The laser lamp that above-mentioned wavelength is 405nm is substituted for the laser lamp that wavelength is 450nm, executes mobile phone in step 301
Operating process, obtain location of pixels P of the emergent light in photo for the laser lamp that wavelength is 450nm450。
In the state that the emergent light of 303. recording wavelength 532nm laser lamps does not have a nanogold reaction solution in cuvette
Location of pixels P in photo532；
The laser lamp that above-mentioned wavelength is 450nm is substituted for the laser lamp that wavelength is 532nm, executes mobile phone in step 301
Operating process, obtain location of pixels P of the emergent light in photo for the laser lamp that wavelength is 532nm532。
In the state that the emergent light of 304. recording wavelength 650nm laser lamps does not have a nanogold reaction solution in cuvette
Location of pixels P in photo650；
The laser lamp that above-mentioned wavelength is 532nm is replaced with into the laser lamp that wavelength is 650nm, executes mobile phone in step 301
Operating process, obtain location of pixels P of the emergent light in photo for the laser lamp that wavelength is 650nm650。
Four points (405, P that the spectra collection module of 305. mobile phones obtains above step405), (450, P450), (532,
P532) and (650, P650) linear fit is carried out, obtain the relationship between wavelength and pixel.
Spectra collection module obtains the relationship between wavelength and pixel, and lamp source one is replaced back full spectrum lamp source, main menu
Module sends selection detection pattern instruction to lamp source control module, and detection starts.
Referring to Fig. 4, below by taking determinand is crystal methamphetamine as an example, absorption and fluorescence spectrum in the present embodiment are specifically described
The detection process of detection device.
In the present embodiment, the process of making nanogold reaction solution is：First plus the crystal methamphetamine nucleic acid of 5 μM of 10 μ L
Aptamers (end modified to have excitation for 495nm, be emitted as the fluorescence probe of 517nm) contain determinand methylbenzene third to 120 μ L
In the sample solution of amine, after reacting at room temperature 5min, the nano-Au solution of 150 μ L9nM is added, is reacting after five minutes, 20 μ L is added
The NaCl solution of 500nM.
When absorbing and fluorescence spectrum detecting device uses colorimetric detection pattern, detailed process is as follows：
Lamp source control module in 401. optical sensing attachmentes is controlled according to the instruction that the main menu block in mobile phone is sent
Lamp source one works；
Main menu block in mobile phone sends selection detection pattern instruction to lamp source control module, lamp source control module according to
Detection pattern instruction control lamp source one is selected to work, that is to say, that emergent light is the white light of full spectrum.
402. nanogold reaction solution is not added in cuvette and is added under nanogold reaction solution two states, light
It composes acquisition module and obtains spectrogram S0With spectrogram S；
The light that lamp source one is emitted passes through cuvette, aperture, condenser lens one, speculum, condenser lens two and diffraction grating
After reach CCD camera.
In the state of in cuvette that nanogold reaction solution is not added, the main menu block in mobile phone is into mobile phone
Spectra collection module sends spectra collection instruction, and spectra collection module instructs the CCD camera of control mobile phone according to spectra collection
It takes pictures, obtains the luminous intensity of each pixel in photo, luminous intensity is depicted as according to the relationship between wavelength and pixel by spectrogram
S0。
In the state that nanogold reaction solution is added in cuvette, the main menu block in mobile phone is again into mobile phone
Spectra collection module sends spectra collection instruction, and spectra collection module instructs the CCD camera of control mobile phone according to spectra collection
It takes pictures, obtains the luminous intensity of each pixel in photo, luminous intensity is depicted as according to the relationship between wavelength and pixel by spectrogram
S。
403. according to spectrogram S0And S, colorimetric module obtain the concentration of crystal methamphetamine；
Colorimetric module in mobile phone is according to spectrogram S0The luminous intensity I at wavelength 520nm is respectively obtained with spectrogram S0GWith
IG, also according to spectrogram S0The luminous intensity I at wavelength 625nm is respectively obtained with spectrogram S0RAnd IR, then according to lambert Bill
Law formula respectively obtains absorbance A of the nanogold reaction solution at wavelength 625nm625With the absorbance at wavelength 520nm
A520, and then obtain absorbance ratio A625/A520, finally according to the linear pass between absorbance ratio and crystal methamphetamine concentration
System obtains the concentration of crystal methamphetamine.
Linear relationship between absorbance ratio and crystal methamphetamine concentration refers to：Absorbance ratio is with methylbenzene third
The increase of amine concentration and linearly increase, can specifically pass through the standard of the linear relationship of preset ratio and concentration in colorimetric module
Curve conversion obtains.
The concentration of crystal methamphetamine is sent to concentration display module by colorimetric module.
404. concentration display module display densities on mobile phone screen, network is published on by network share module；
When absorbing and fluorescence spectrum detecting device uses fluoroscopic examination pattern, detailed process is as follows：
Lamp source control module in 405. optical sensing attachmentes is controlled according to the instruction that the main menu block in mobile phone is sent
Lamp source two works；
Main menu block in mobile phone sends selection detection pattern instruction to lamp source control module, lamp source control module according to
Detection pattern instruction control lamp source two is selected to work, the wavelength of the emergent light of lamp source two is 495nm, excites the solution in cuvette
Generate fluorescence.
406. are added without nanogold reaction solution in cuvette and are added under the two states of nanogold reaction solution, light
It composes acquisition module and obtains spectrogram S'0And S'；
Fluorescence reaches CCD after cuvette, aperture, condenser lens one, speculum, condenser lens two and diffraction grating and takes the photograph
As head.The execution flow of step 406 is identical with the execution flow of step 402.
407. according to spectrogram S0And S', fluorescent moieties obtain the concentration of crystal methamphetamine；
Fluorescent moieties in mobile phone according to acquisition spectrogram S'0And S', respectively obtain the luminous intensity F at wavelength 517nm0
And F, the relative changing value for the fluorescence intensity that nanogold reaction solution generates under the irradiation of lamp source two is then obtained, according to opposite
Linear relationship between changing value and crystal methamphetamine concentration obtains the concentration of crystal methamphetamine.
Linear relationship between relative changing value and crystal methamphetamine concentration refers to：Relative changing value is with methylbenzene third
The increase of amine concentration and linearly increase, can specifically pass through the linear relationship of preset relative changing value and concentration in fluorescent moieties
Standard curve conversion obtain.
The concentration of crystal methamphetamine is sent to concentration display module by fluorescent moieties.
Abovementioned steps 404 are finally executed, concentration display module shows the methyl provided by fluorescent moieties on mobile phone screen
The concentration of amphetamine is published on network by network share module.
It is provided for the embodiments of the invention a kind of absorption based on mobile intelligent terminal and fluorescence spectrum detection dress above
It sets and is described in detail, principle and implementation of the present invention are described for specific case used herein, above
The explanation of embodiment is merely used to help understand the method and its core concept of the present invention；Meanwhile for the general skill of this field
Art personnel, according to the thought of the present invention, there will be changes in the specific implementation manner and application range, for example, this implementation
In example, the ratio of absorbance of the nanogold reaction solution at 625nm and 520nm may be A520/A625, at this time ratio with it is dense
The linear relationship of degree is：Ratio is linearly reduced with the increase of concentration；In another example others can be modified on aptamer
Fluorescence probe selects corresponding laser or LED as lamp source two, the fluorescence is selected in fluorescent moieties according to its excitation wavelength
The relative changing value of the transmitted wave strong point fluorescence intensity of probe calculates testing concentration；In another example the present invention detects specificity
The specificity from aptamer, pass through and replace nucleic acid aptamer sequence, you can establish for other in other field
The specific detection of substance；For another example absorption and fluorescence spectrum detecting device based on mobile intelligent terminal can also by except
Other substances other than nano Au particle realize colorimetric detection, if according to the change of its characteristic absorption peak position, than
The luminous intensity corresponded at characteristic absorption peak wavelength is chosen in color module to carry out that absorbance is asked to calculate.In conclusion this theory
Bright book content should not be construed as limiting the invention.
Claims (10)
1. a kind of absorption and fluorescence spectrum detecting device based on mobile intelligent terminal, which is characterized in that including：It is taken the photograph with CCD
It is installed as the mobile intelligent terminal of head, touching display screen and central processing unit, optical sensing attachment, by the optical sensing attachment
In the adapter on mobile intelligent terminal；The optical sensing attachment includes for the lamp source one of colorimetric detection pattern and for glimmering
The lamp source two of light detection pattern, the emitting light path one of lamp source one are mutually perpendicular to the emitting light path two of lamp source two；The optics passes
It further includes cuvette to feel attachment, and the cuvette and the emitting light path one and the emitting light path two are coaxial, the lamp source one
Emergent light for full spectrum light source, the lamp source two excites the solution in the cuvette to generate the fluorescence for detecting required wavelength,
The optical sensing attachment further includes in the cuvette rear end and the speculum that is arranged along the emitting light path one, the outgoing
Light path one and the speculum there are incidence angle, the optical sensing attachment further include setting the CCD camera with it is described
Diffraction grating between speculum hangs down through cuvette and along the emergent light of the emitting light path one after speculum reflection
The straight incident diffraction grating, through the diffraction grating, on CCD camera, the optical sensing attachment further includes diffraction again
Lamp source control module, the lamp source control module are connect with each lamp source circuit, control the opening and closing of each lamp source respectively；
The mobile intelligent terminal includes main menu block, spectra collection module, colorimetric module, fluorescent moieties, concentration display mould
Block；The main menu block to the lamp source control module send selection detection pattern instruction, the lamp source control module according to
The selection detection pattern instruction selects the lamp source one or the lamp source two to work, when two work of the lamp source one or the lamp source
When making, in the case where the cuvette is not added solution to be measured or solution two states to be measured are added, the main menu block is to institute
It states spectra collection module and sends spectra collection instruction, the spectra collection module controls the shifting according to spectra collection instruction
The CCD camera of dynamic intelligent terminal takes pictures, obtains the luminous intensity of each pixel in photo, according between wavelength and pixel
The luminous intensity is depicted as spectrogram, is sent to the colorimetric module or the fluorescent moieties according to selection detection pattern by relationship
The spectrogram, the colorimetric module obtain according to the spectrogram and solution to be measured or addition two kinds of solution to be measured are added no
The luminous intensity of two specific wavelengths under state obtains solution to be measured in two specific wavelengths further according to langbobier law formula
The absorbance at place is obtained according to the linear relationship between the ratio and testing concentration of the absorbance of described two certain wave strong points
The concentration of determinand, it is to be measured molten in no addition that the fluorescent moieties obtain a certain certain wave strong point luminous intensity in the spectrogram
Liquid or the relative changing value being added under solution two states to be measured, according between the relative changing value and solution concentration to be measured
Linear relationship obtains the concentration of solution to be measured, and the concentration display module receives the colorimetric module or fluorescent moieties hair
The concentration sent, shows the concentration on the touching display screen of the mobile intelligent terminal.
2. absorption according to claim 1 and fluorescence spectrum detecting device, which is characterized in that the optical sensing attachment is also
Including condenser lens one, the condenser lens one it is pluggable be arranged between the speculum and the cuvette, along it is described go out
The emergent light for penetrating light path one is focused on after the condenser lens one on the speculum.
3. absorption according to claim 2 and fluorescence spectrum detecting device, which is characterized in that the optical sensing attachment is also
It is arranged between the condenser lens one and the cuvette and the adjustable port coaxial with the emitting light path one including pluggable
The aperture of diameter size, the aperture can control the emergent light along the emitting light path one through the emergent light light after the aperture
Amount.
4. absorption according to claim 1 and fluorescence spectrum detecting device, which is characterized in that the optical sensing attachment is also
Including condenser lens two, the condenser lens two is pluggable to be arranged between the diffraction grating and the speculum, along described
The emergent light of emitting light path one focuses on the diffraction grating after speculum reflection using the condenser lens two
Surface.
5. absorption according to any one of claim 1 to 4 and fluorescence spectrum detecting device, which is characterized in that the shifting
Dynamic intelligent terminal further includes network share module, and the concentration display module sends the concentration to the network share module,
The network share module issues the concentration received on network.
6. absorption as claimed in claim 5 and fluorescence spectrum detecting device, which is characterized in that the lamp source one is full spectrum
LED light；The lamp source two is the LED light or laser lamp of specific wavelength.
7. absorption as claimed in claim 6 and fluorescence spectrum detecting device, which is characterized in that the collector lens one and institute
It is planoconvex spotlight to state collector lens two, and thickness is 1mm, and diameter is 10mm, and focal length is respectively 25mm and 15mm；Described
Speculum is plane mirror, thickness 1mm, a diameter of 10mm.
8. absorption as claimed in claim 7 and fluorescence spectrum detecting device, which is characterized in that the diffraction grating is transmission
Grating, thickness 2mm, a diameter of 10mm, line number is not less than 1200 lines/mm.
9. absorption as claimed in claim 8 and fluorescence spectrum detecting device, which is characterized in that the lamp source one and the lamp source
Two can also be connected by circuit with the USB interface of the mobile intelligent terminal, by the battery of the mobile intelligent terminal to institute
It states lamp source one and the lamp source two is powered.
10. absorption according to claim 9 and fluorescence spectrum detecting device, which is characterized in that when absorption and fluorescence spectrum
Detection device needs timing, and lamp source one is replaced with wavelength point successively by full spectrum lamp source according to the sequence of wavelength from small to large
Not Wei 405nm, 450nm, 532nm, 650nm laser lamp, laser lamp each time unlatching after, do not have in the cuvette
In the state of solution to be measured, the main menu block sends spectra collection instruction to the spectra collection module, and the spectrum is adopted
Collection module controls the CCD camera and takes pictures, and location of pixels of the emergent light of current lamp source in photo is recorded, according to institute
Four location of pixels of record carry out linear fit, and the spectra collection module obtains the relationship between wavelength and pixel,
Then lamp source one is replaced into back full spectrum lamp source, the main menu block sends selection detection pattern to the lamp source control module
Instruction.
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| PCT/CN2018/082115 WO2018188524A1 (en) | 2017-04-14 | 2018-04-08 | Absorption and fluorescence spectrum detection apparatus based on mobile intelligent terminal |
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| CN108731805B (en) | 2023-06-02 |
| WO2018188524A1 (en) | 2018-10-18 |
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