CN108641914B - Circulating chlorella breeds separating centrifuge - Google Patents
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Abstract
The invention discloses a circulating chlorella proliferation separator which comprises a growth device, a proliferation supply device and a separation and circulation device, wherein the growth device comprises a growth groove and a groove top shielding mechanism, the upper part of the growth groove is open, the proliferation device is communicated with the growth device through a pipeline, the proliferation device comprises a plurality of proliferation tanks, and each proliferation tank is provided with a pipeline with an independent feeding valve; the proliferation supply device comprises a nutrient mixing tank, a nutrient tank and a solution tank, wherein separate pipelines are arranged between the nutrient mixing tank and the nutrient tank as well as between the nutrient mixing tank and the solution tank, and a pipeline with an independent supply valve is arranged between the proliferation supply device and each proliferation tank; the separation circulating device comprises a separation tank and a circulating pipeline, the separation tank is connected with all the propagation tanks, and the circulating pipeline is communicated with the separation tank and the propagation tanks. The invention can make chlorella grow in high quality under the state of utilizing photosynthesis to nourish, and then the chlorella is diluted and put into nutrient solution to utilize heterotrophy to proliferate efficiently.
Description
Technical Field
The invention relates to the field of green algae cultivation, in particular to a circulating type chlorella proliferation separator
Background
The chlorella is a kind of globular unicellular freshwater algae of Chlorella of Chlorophyta, with a diameter of 3-8 microns, which is one of the earliest lives on earth, and appears 20 hundred million years ago, and is a high-efficiency photosynthetic plant, which grows and breeds by photoautotrophy and has extremely wide distribution.
Currently, there are about 10 species of chlorella known in the world, and several hundred species of chlorella are known. Chlorella is widely distributed in nature, and the types of chlorella in fresh water areas are the most; the culture is easy, and the light energy can be used for autotrophy and the organic carbon source can be used for growth and propagation under the heterotrophic condition; and the growth and reproduction speed is high, and the growth and reproduction speed is the only organism which can increase 4 times in 20 hours in the animals and plants on the earth, so the application value is very high. Common species in China include chlorella pyrenoidosa, chlorella ellipsoidea, chlorella vulgaris and the like, wherein the chlorella pyrenoidosa has high protein content and highest nutritional value.
Photoautotrophic culture methods currently include open pond culture and closed culture. The former has simple equipment, low technical content and low investment, but the yield is low and is easily influenced by natural conditions. The latter mainly comprises two culture modes of a closed fermentation tank and a glass pipeline photosynthetic bioreactor. Compared with the former, the nutrient content of the chlorella after closed heterotrophic culture is reduced, but the yield of the heterotrophic algae per unit volume is 10 times that of the autotrophic algae under the same culture time. The yield of the single-cell protein of the chlorella per unit volume under heterotrophic conditions is still higher than that of the autotrophic chlorella.
At present, no device can combine the advantages of an open type culture mode and a closed type culture mode, so that the content of protein in chlorella per se is high, the quality of algae population is high, and the proliferation is fast.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provides a circulating type chlorella breeding separator, which is provided with an open type growing device, so that chlorella can fully grow in an autotrophic environment by utilizing illumination to promote the quality of chlorella population, cultured chlorella population is dispersed into each breeding tank of the breeding device, light is separated after organic carbon sources are mixed, heterotrophy is fully utilized to carry out high-speed breeding, and the produced chlorella has high protein content, high population breeding speed and high yield.
In order to achieve the purpose and achieve the technical effect, the technical scheme of the invention is as follows: a circulating chlorella breeder separator comprising a growing device, a breeding supply device and a separation and circulation device, characterized in that: the growth device comprises a growth tank and a tank top shielding mechanism, wherein the upper part of the growth tank is open, the tank top shielding mechanism is arranged at the upper part of the growth tank, and air increasing holes are uniformly formed in the inner tank wall of the growth tank;
the proliferation device is communicated with the growth device through a pipeline and comprises a plurality of proliferation tanks, and each proliferation tank is provided with a pipeline with an independent feeding valve;
the proliferation supply device comprises a nutrient mixing tank, a nutrient tank and a solution tank, wherein separate pipelines are arranged between the nutrient mixing tank and the nutrient tank as well as between the nutrient mixing tank and the solution tank, and a pipeline growth tank with an independent supply valve is arranged between the proliferation supply device and each proliferation tank;
the separation circulating device comprises a separation tank and a circulating pipeline, the separation tank is connected with all the propagation tanks, and the circulating pipeline is communicated with the separation tank and the growth tanks.
In a preferred embodiment of the present invention, the growth trough further comprises a first illumination mechanism disposed at an intermediate position within the growth trough.
In a preferred embodiment of the present invention, the apparatus further comprises a second illumination mechanism connected to a side of the top shielding mechanism facing the growth trough.
In a preferred embodiment of the present invention, the growth tank further comprises a tank top shielding mechanism capable of completely covering the notch formed at the upper part of the growth tank, and the tank top shielding mechanism is provided with a dimming glass layer.
In a preferred embodiment of the invention, the pipeline between the growing device and the proliferation device is provided with a secondary nutrition mixing mechanism, the secondary nutrition mixing mechanism comprises a secondary mixing tank and a secondary supplement tank, and the secondary supplement tank is separately communicated with the secondary mixing tank.
In a preferred embodiment of the present invention, the proliferation tank further comprises a temperature balancing mechanism and a pH value balancing mechanism arranged therein.
In a preferred embodiment of the present invention, the proliferation tank is opaque to light.
In a preferred embodiment of the present invention, the separation tank is provided with a centrifugal mechanism, and a pipeline between the separation tank and the proliferation tank is provided with a centrifugal control valve.
In a preferred embodiment of the present invention, the circulation pipe is provided with a circulation valve in a pipe connecting the separation tank and the growth tank.
In a preferred embodiment of the present invention, the centrifugal mechanism further comprises a drying mechanism.
The invention has the beneficial effects that:
the chlorella culture device is provided with a growth device and a proliferation device, the chlorella can be arranged in an open growth groove at the middle upper part of the growth device for autotrophy by utilizing light irradiation, the content of nutrient components and proteins in the chlorella can be improved, the population quality of the chlorella can also be improved, a high-quality chlorella community can be obtained, then the chlorella community is dispersed into each proliferation tank in the proliferation device, light rays are separated after organic carbon sources are mixed, and high-speed proliferation is carried out by fully utilizing heterotrophy.
The device is provided with the proliferation supply device, nutrient substances and solution are separated and supplied, the shelf life of the mixed nutrient solution can be prolonged, the phenomenon that mixed bacteria absorb nutrient components to influence the growth of chlorella is avoided, meanwhile, the material separation is convenient, the blending and the supplement are convenient, the repeated blending is not needed, the device supplements according to the use quantity and the actual needs of the proliferation tanks, the utilization rate of raw materials is high, and the management labor is saved.
And thirdly, the separation circulating device is arranged in the culture medium separation device and comprises a separation tank and a circulating pipeline, the separation tank can be used for dehydrating and separating the chlorella mixed in the culture medium, the culture medium is directly separated and then is supplemented into the growth grooves through the circulating pipeline, the material recycling rate is high, and the separated chlorella is convenient for subsequent processing operation.
The chlorella cultivation and separation device is characterized in that a growth device, a proliferation supply device and a separation circulation device are arranged, pipelines are arranged between the growth device and the proliferation device, between the proliferation supply device and the separation circulation device, and the autotrophic culture, the shunt culture, the heterotrophic culture and the dehydration separation of the chlorella are all automatic operations, so that the chlorella cultivation and separation device is low in dependence on human power, high in automation degree, short in cultivation period, and high in yield and quality of the chlorella.
And a secondary nutrition mixing mechanism is arranged on a pipeline between the growth device and the proliferation device, the secondary nutrition mixing mechanism comprises a secondary mixing tank and a secondary supplement tank, the secondary supplement tank is independently communicated with the secondary mixing tank, and the secondary nutrition mixing mechanism can mix and supplement a small-range nutrient solution in advance before chlorella colonies which are shunted out from the growth tank fall into each proliferation tank which is dispersed in the proliferation device, so that the chlorella can adapt to a heterotrophic culture environment in advance, and the self state of the chlorella is adjusted, so that the chlorella can adapt to the heterotrophic environment more quickly. The proliferation speed is increased, so that the yield and the quality of the chlorella are improved, and secondly, the setting of the secondary nutrition mixing mechanism can facilitate the holding of the mixed culture concentration of the chlorella, so that the quantity and the quality of the chlorella put into each proliferation tank are balanced, the quality of the chlorella cultured uniformly is balanced, and the uniform management is facilitated.
The device comprises a plurality of proliferation tanks, each proliferation tank is provided with a pipeline with an independent feeding valve, a pipeline growth tank with an independent supply valve is arranged between each proliferation tank and the proliferation supply device, and a separation tank is connected with all the proliferation tanks.
The foregoing description is only an overview of the technical solutions of the present invention, and in order to make the technical solutions of the present invention more clearly understood and to implement them in accordance with the contents of the description, the following detailed description is given with reference to the preferred embodiments of the present invention and the accompanying drawings. The detailed description of the present invention is given in detail by the following examples and the accompanying drawings.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments of the present invention will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a block diagram of a preferred embodiment of the present invention;
FIG. 2 is a block diagram of a growing apparatus according to a preferred embodiment of the present invention;
FIG. 3 is a block diagram of a proliferation apparatus in accordance with a preferred embodiment of the present invention;
fig. 4 is a structural view of a separation cycle apparatus according to a preferred embodiment of the present invention.
In FIGS. 1-4: 1. the device comprises a growing device, 2, a breeding device, 3, a breeding supply device, 4, a separation circulating device, 5, a growing tank, 6, a tank top shielding mechanism, 7, a gas increasing hole, 8, a breeding tank, 9, a feeding valve, 10, a nutrient mixing tank, 11, a nutrition tank, 12, a solution tank, 13, a nutrient supply valve, 14, a separation tank, 15, a circulating pipeline, 16, a first illumination mechanism, 17, a second illumination mechanism, 18, a secondary mixing tank, 19, a secondary supplement tank, 20, a circulating valve, 21 and a centrifugal control valve.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
As shown in fig. 1 to 4, the present embodiment discloses a circulating chlorella growth separator comprising a growth device 1, a growth device 2, a growth supply device 3, and a separation circulation device 4, wherein: the growth device 1 comprises a growth tank 5 and a tank top shielding mechanism 6, wherein the upper part of the growth tank 5 is open, the tank top shielding mechanism 6 is arranged at the upper part of the growth tank 5, and gas increasing holes 7 are uniformly arranged on the inner tank wall of the growth tank 5; the proliferation device 2 is communicated with the growth device 1 through a pipeline, the proliferation device 2 comprises a plurality of proliferation tanks 8, and each proliferation tank 8 is provided with a pipeline with an independent feeding valve 9; the proliferation supply device 3 comprises a nutrient mixing tank 10, a nutrient tank 11 and a solution tank 12, wherein separate pipelines are arranged between the nutrient mixing tank 10 and the nutrient tank 11 as well as between the nutrient mixing tank 12 and the solution tank 11, and a pipeline growth tank 5 with an independent nutrient supply valve 13 is arranged between the proliferation supply device 3 and each proliferation tank 8; the separation and circulation device 4 comprises a separation tank 14 and a circulation pipeline 15, the separation tank 14 is connected with all the propagation tanks 8, and the circulation pipeline 15 is communicated with the separation tank 14 and the growth tank 5. The invention is provided with a growth device 1 and a proliferation device 2, the chlorella can be arranged in an open growth groove 5 at the middle upper part of the growth device 1 for autotrophy by utilizing illumination, the content of nutrient components and proteins in the chlorella can be improved, the population quality of the chlorella can also be improved, a high-quality chlorella community can be obtained, then the chlorella community is dispersed into each proliferation tank 8 in the proliferation device 2, light rays are separated after organic carbon sources are mixed, and high-speed proliferation is carried out by fully utilizing heterotrophy, so that the chlorella cultured by combining the autotrophy and heterotrophy modes has high content of self proteins and nutrient components, the population proliferation speed is high, the yield is high, and meanwhile, the degradation of chlorella offspring is slow after multiple times of cyclic culture, and the chlorella offspring can be continuously recycled. The nutrient substances and the solution are separated and supplied, the shelf life of the mixed nutrient solution can be prolonged, the situation that the growth of chlorella is influenced by the absorption of nutrient components by sundry bacteria is avoided, meanwhile, the materials are separated, the preparation and the supplement are convenient, the repeated preparation is not needed, the device is supplemented according to the use quantity and the actual needs of the proliferation tank 8, the utilization rate of raw materials is high, and the management labor is saved. The separation and circulation device 4 comprises a separation tank 14 and a circulation pipeline 15, the separation tank 14 can be used for dehydrating and separating the chlorella mixed in the culture solution, the culture solution is directly separated and then is supplemented into the growth tank 5 through the circulation pipeline 15, the material recycling rate is high, and the separated chlorella is convenient for subsequent processing operation. The growth device 1 and the multiplication device 2, the multiplication supply device 3 and the separation circulating device 4 are connected with pipelines, the autotrophic culture, the shunt culture, the heterotrophic culture and the dehydration separation of the chlorella are all automatic operations, the dependence on manpower is low, the automation degree is high, the culture period is short, and the yield and the quality of the chlorella are high. A secondary nutrition mixing mechanism is arranged on a pipeline between the growth device 1 and the proliferation device 2, the secondary nutrition mixing mechanism comprises a secondary mixing tank 18 and a secondary supplement tank 19, the secondary supplement tank 19 is independently communicated with the secondary mixing tank 18, and the secondary nutrition mixing mechanism can mix and supplement a small range of nutrient solution in advance before chlorella groups flowing out of the growth tank 5 fall into each proliferation tank 8 dispersed in the proliferation device 2, so that the chlorella can adapt to a heterotrophic culture environment in advance, the self state of the chlorella is adjusted, and the chlorella can adapt to the heterotrophic environment more quickly. The proliferation speed is increased, so that the yield and the quality of the chlorella are improved, and secondly, the setting of the secondary nutrition mixing mechanism can facilitate the holding of the mixed culture concentration of the chlorella, so that the quantity and the quality of the chlorella put into each proliferation tank 8 are balanced, the quality of the cultured chlorella is balanced, and the uniform management is facilitated. Proliferation device 2 includes a plurality of proliferation jar 8, each proliferation jar 8 all is provided with the pipeline of taking independent feeding valve 9, all set up the pipeline growth groove 5 that has independent support valve 13 between proliferation feed arrangement 3 and the proliferation jar 8 of each, knockout drum 14 all is connected with all proliferation jars 8, such independent pipeline design can make all proliferation jars 8 all have independent feeding in the proliferation device 2, the ejection of compact is the culture environment in addition, can the exclusive use in actual operation, also can use in batches, the rational utilization device of maximum, when guaranteeing production, the running energy consumption of economizer. The air increasing holes 7 can supplement sufficient air to the chlorella in the growth grooves 5, improve the photosynthesis effect of the chlorella, and meanwhile, the air flow emitted from the air increasing holes 7 can promote the exchange of the solution in the growth grooves 5 and the distribution of the chlorella, so that the chlorella in the grooves can obtain sufficient autotrophic conditions.
In use, the first illumination means 16 is provided in the growth tank 5 at an intermediate position. The first illumination mechanism 16 can supplement light to the chlorella in the elongated trough 5, improve the culture density of the chlorella, and improve the photosynthesis effect of the chlorella.
When the invention is used, one side of the groove top shielding mechanism 6 facing the growth groove 5 is connected with a second illumination mechanism 17. The second illumination mechanism 17 can assist sunlight or supplement light rays for chlorella with the groove opening of the elongated trough 5 under the condition of no illumination, improve the culture density of the chlorella and improve the photosynthesis effect of the chlorella.
When the invention is used, the groove top shielding mechanism 6 can completely cover the notch formed at the upper part of the growing groove 5, and the groove top shielding mechanism 6 is internally provided with a dimming glass layer. And under the condition that the external illumination intensity is too strong, the transmittance of the dimming glass layer is adjusted to ensure that the chlorella can obtain the optimal illumination intensity.
When the device is used, a secondary nutrition mixing mechanism is arranged on a pipeline between the growth device 1 and the proliferation device 2, the secondary nutrition mixing mechanism comprises a secondary mixing tank 18 and a secondary supplement tank 19, and the secondary supplement tank 19 is separately communicated with the secondary mixing tank 18. The secondary nutrient mixing means mixes and supplements the chlorella flock flowing out of the growth tank 5 with a small range of nutrient solution in advance before the chlorella flock is dispersed in the respective proliferation tanks 8 of the proliferation apparatus 2, so that the chlorella can adapt to the heterotrophic culture environment in advance, and the self state of the chlorella can be adjusted, so that the chlorella can adapt to the heterotrophic environment more quickly. The proliferation speed is increased, so that the yield and the quality of the chlorella are improved, and secondly, the setting of the secondary nutrition mixing mechanism can facilitate the holding of the mixed culture concentration of the chlorella, so that the quantity and the quality of the chlorella put into each proliferation tank 8 are balanced, the quality of the cultured chlorella is balanced, and the uniform management is facilitated.
When the device is used, the proliferation tank 8 is internally provided with a temperature balance mechanism and a pH value balance mechanism. The temperature balance mechanism can maintain the temperature in the proliferation tank 8 and ensure that the chlorella always has the optimal growth temperature, and the pH value balance mechanism can adjust the pH value in the proliferation tank 8, so that the pH value in the proliferation tank 8 is always maintained at an ideal set value and the chlorella always has the optimal growth pH value.
When the invention is used, the proliferation tank 8 is not light-tight as a whole. Light is prevented from entering the proliferation tank 8, so that the chlorella is subjected to photosynthesis, heterotrophic proliferation is not carried out, the yield of the chlorella is influenced, the lighttight proliferation tank 8 can ensure that the chlorella is only heterotrophic in the tank, and the yield of the device is ensured.
When the invention is used, a centrifugal mechanism is arranged in the separation tank 14, a centrifugal control valve 21 is arranged on a pipeline between the separation tank 14 and the proliferation tank 8, and a circulating valve 20 is arranged on a pipeline between the separation tank 14 and the growth tank 5 and connected with the circulating pipeline 15. When the centrifugal mechanism carries out centrifugal treatment on chlorella, the inside of the device is isolated from the outside, and the centrifugal dehydration separation effect is ensured to the maximum extent.
When the centrifugal drying machine is used, the centrifugal mechanism is also provided with a drying mechanism. The drying mechanism can dry the chlorella after centrifugal separation, so that water carrying is reduced, and subsequent operation is facilitated.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (7)
1. A circulating chlorella breeder separator comprising a growing device, a breeding supply device and a separation and circulation device, characterized in that: the growth device comprises a growth tank and a tank top shielding mechanism, wherein the upper part of the growth tank is open, the tank top shielding mechanism is arranged at the upper part of the growth tank, and air increasing holes are uniformly formed in the inner tank wall of the growth tank;
the proliferation device is communicated with the growth device through a pipeline and comprises a plurality of proliferation tanks, and each proliferation tank is provided with a pipeline with an independent feeding valve;
the breeding supply device comprises a nutrient mixing tank, a nutrient tank and a solution tank, wherein the nutrient mixing tank is arranged at one end of the nutrient mixing tank, the solution tank is arranged at the other end of the nutrient mixing tank, the nutrient tank and the solution tank are connected through separate pipelines, the nutrient mixing tank is connected with the breeding tank through a pipeline, and the pipelines between the nutrient mixing tank and the breeding tank are provided with a nutrient supply valve;
the separation circulating device comprises a separation tank and a circulating pipeline, the separation tank is connected with all the propagation tanks, and the circulating pipeline is communicated with the separation tank and the growth tanks;
a secondary nutrition mixing mechanism is arranged on a pipeline between the growing device and the proliferation device, the secondary nutrition mixing mechanism comprises a secondary mixing tank and a secondary supplement tank, the secondary supplement tank is separately communicated with the secondary mixing tank, the secondary mixing tank is communicated with the proliferation tank through a pipeline, the pipeline between the secondary mixing tank and the proliferation tank is provided with the feeding valve, and the growing groove is communicated with the secondary mixing tank through a pipeline;
a centrifugal mechanism is arranged in the separation tank, and a centrifugal control valve is arranged on a pipeline between the separation tank and the proliferation tank;
the circulation pipe is provided with a circulation valve on a pipe connecting the separation tank and the growth tank.
2. The circulating chlorella propagation separator according to claim 1, wherein: and a first illumination mechanism is arranged at the middle position in the growth groove.
3. The circulating chlorella propagation separator according to claim 1, wherein: one side of the groove top shielding mechanism facing the growth groove is connected with a second illumination mechanism.
4. The circulating chlorella propagation separator according to claim 1, wherein: the groove top shielding mechanism can completely cover the groove opening formed in the upper portion of the growth groove, and a dimming glass layer is arranged in the groove top shielding mechanism.
5. The circulating chlorella propagation separator according to claim 1, wherein: and a temperature balance mechanism and a pH value balance mechanism are arranged in the proliferation tank.
6. The circulating chlorella propagation separator according to claim 1, wherein: the proliferation tank is light-proof as a whole.
7. The circulating chlorella propagation separator according to claim 1, wherein: and a drying mechanism is also arranged in the centrifugal mechanism.
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| CN103627620A (en) * | 2012-08-28 | 2014-03-12 | 西门子公司 | Method and apparatus for producing lipids through microalgae culture |
| CN204625611U (en) * | 2015-05-08 | 2015-09-09 | 天津农学院 | A kind of chlorella culture device |
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