CN108641914A - A kind of circulating chlorella proliferation seperator - Google Patents
A kind of circulating chlorella proliferation seperator Download PDFInfo
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- CN108641914A CN108641914A CN201810257724.2A CN201810257724A CN108641914A CN 108641914 A CN108641914 A CN 108641914A CN 201810257724 A CN201810257724 A CN 201810257724A CN 108641914 A CN108641914 A CN 108641914A
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- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
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Abstract
The invention discloses a kind of circulating chlorellas to be proliferated seperator, including grower, proliferation device, proliferation feedway and separating circulating device, grower includes growth slot and groove top means for screening, it is open to grow slot top, it is connected to by pipeline between proliferation device and grower, proliferation device includes several proliferation tanks, each proliferation tank is both provided with the pipeline with independent pan feeding valve；It includes nutriment blending tank, nutrition tank, NaOH solution tank NaOH to be proliferated feedway, and individual pipeline is both provided between nutriment blending tank and nutrition tank and NaOH solution tank NaOH, is proliferated between feedway and the proliferation tank of each and is respectively provided with the independent pipeline for supporting valve；Separating circulating device includes knockout drum and circulating line, and knockout drum is all connected with all proliferation tanks, and circulating line is connected to knockout drum and growth slot.The present invention can make chlorella grow up using high quality under photosynthesis autotrophy state, and then dilution, which is put into nutrient solution, utilizes heterotrophism high efficiently multiplying.
Description
Technical field
The present invention relates to green algas to cultivate field, and in particular to a kind of circulating chlorella proliferation seperator
Background technology
Chlorella is the general natural disposition monoplast green alga of Chlorophyta Chlorella, is a kind of spherical unicellular fresh water algae, diameter
3-8 microns, be one of life earliest on the earth, is a kind of efficient photosynthetic plant before appearing in more than 20 hundred million years, with it is photosynthetic from
Growth and breeding is supported, distributed pole is wide.
Current about 10 kinds of chlorella known in the world, in addition its mutation is up to as many as hundreds of.Chlorella is widely distributed
In nature, with freshwater most species；It is easy to cultivate, photoautotrophy can not only be utilized, moreover it is possible to be utilized under the conditions of heterotrophism
Organic carbon source is grown, is bred；And growth and breeding speed is fast, is that uniquely can increase by 4 times in 20h in animals and plants on the earth
Biology, so its application value is very high.The common type in China has chlorella pyrenoidosa, chlorella ellipsoidea, chlorella vulgaris etc.,
Wherein chlorella pyrenoidosa protein content is high, nutritive value highest.
Photoautotrophic training method includes open pond culture and closed culture at present.The former equipment is simple, technology contains
It measures low, small investment, but low output and is easily influenced by natural conditions.The latter mainly has sealed fermenting tank and the photosynthetic life of glass pipe
Object bioreactor culture two ways.Compared with the former, the nutrient composition content of the chlorella after closed Heterotrophic culture decreases,
But under identical incubation time, the harvest yield of unit volume heterotrophism algae is 10 times of autotrophy algae.Unit volume is small under the conditions of heterotrophism
Ball algae single cell protein yield is still higher than autotrophy chlorella.
Current not a kind of device can allow chlorella itself in conjunction with the advantages of open and closed culture two ways
Protein content is high, and algal populations quality is high, and proliferation is fast.
Invention content
It is an object of the invention to overcome problem above of the existing technology, a kind of circulating chlorella proliferation is provided
Seperator is provided with open grower in of the invention, chlorella is made fully to be grown using illumination in autotrophy environment, is promoted
Into chlorella population quality, then cultured chlorella population is distributed in each proliferation tank of proliferation device, has been mixed into
Light to be isolated after machine carbon source, heterotrophism is made full use of to carry out fast breeder, the chlorella oneself protein matter content produced in this way is high,
Population augment speed is fast, and yield is high.
In order to achieve the above objectives, reach above-mentioned technique effect, technical scheme is as follows：A kind of circulating chlorella
It is proliferated seperator, including grower, proliferation device, proliferation feedway and separating circulating device, it is characterised in that：The life
Growth device includes growth slot and groove top means for screening, and the growth slot top is open, and the groove top means for screening setting exists
The growth slot top, it is described growth slot inside cell wall on be evenly arranged with increasing stomata；
It is connected to by pipeline between the proliferation device and the grower, the proliferation device includes several proliferation
Tank, each described proliferation tank are both provided with the pipeline with independent pan feeding valve；
The proliferation feedway includes nutriment blending tank, nutrition tank, NaOH solution tank NaOH, the nutriment blending tank and the nutrition
Individual pipeline is both provided between tank and the NaOH solution tank NaOH, between the proliferation feedway and proliferation tank described in each
It is respectively provided with the independent pipeline growth slot for supporting valve；
The separating circulating device includes knockout drum and circulating line, and the knockout drum connects with all proliferation tanks
It connects, the circulating line is connected to the knockout drum and the growth slot.
In a preferred embodiment of the present invention, further comprise that the middle position in the growth slot is provided with the first light
According to mechanism.
In a preferred embodiment of the present invention, further comprise the groove top means for screening towards the side of the growth slot
It is connected with the second illumination mechanism.
In a preferred embodiment of the present invention, further comprise that the growth slot can be completely covered in the groove top means for screening
The notch that top opens up is provided with dimming glass layer in the groove top means for screening.
In a preferred embodiment of the present invention, further comprise the pipeline between the grower and the proliferation device
On be provided with secondary nutrition mixed organization, the secondary nutrition mixed organization includes secondary blending tank and secondary supplement tank, described
Secondary supplement tank is individually connected to the secondary blending tank.
In a preferred embodiment of the present invention, further comprise being provided with equalized temperature mechanism and soda acid in the proliferation tank
Spend balance mechanism.
In a preferred embodiment of the present invention, further comprise that the proliferation tank is integrally opaque.
In a preferred embodiment of the present invention, further comprise being provided with centrifugal mechanism in the knockout drum, the separation
It is provided with centrifugation control valve on pipeline between tank and the proliferation tank.
In a preferred embodiment of the present invention, further comprise that the circulating line is connecting the knockout drum and the life
It is provided with circulating valve on pipeline between elongated slot.
In a preferred embodiment of the present invention, further comprise being additionally provided with drying mechanism in the centrifugal mechanism.
The beneficial effects of the invention are as follows:
Grower and proliferation device are provided in one, the present invention, chlorella can be first arranged in grower middle and upper part
Autotrophy is carried out to carry out photosynthesis using illumination in open growth slot, promotes nutritional ingredient and albumen in chlorella itself
The content of matter can also promote the population quality of chlorella itself, obtain the chlorella group of high quality, later chlorella group quilt
It is distributed in each proliferation tank in proliferation device, light is isolated after being mixed into organic carbon source, heterotrophism is made full use of to carry out high speed increasing
It grows, the oneself protein matter and nutrition content for the chlorella turned out in this way in conjunction with autotrophy and heterotrophism mode are high, population augment
Speed is fast, and yield is high, while repeatedly after Cyclic culture, chlorella offspring degenerates slowly, can be used with persistent loop.
Secondly, the present invention in be provided with proliferation feedway, by nutriment and solution separation supply, mixing can be extended
The shelf-life of nutrient solution afterwards avoids miscellaneous bacteria therein from absorbing nutritional ingredient, influences the growth of chlorella, while feed separation,
It convenient for allocating and supplementing, need not prepare repeatedly, device is supplemented according to the usage quantity and actual needs of proliferation tank, to original
The utilization rate of material is high, saves management manpower.
Thirdly, be provided with separating circulating device in the present invention, separating circulating device includes knockout drum and circulating line, separation
Tank can carry out dehydration separation to the chlorella being mingled in culture solution, and culture solution is directly separated to and then passed through circulating line
Supplement retrogradation elongated slot, high to the cyclic utilization rate of material, the chlorella after separation is convenient for rear road process operation.
Grower, proliferation device, proliferation feedway and separating circulating device, growth four, are provided in the present invention
It is arranged between device and proliferation device, proliferation feedway and separating circulating device and is connected with pipeline, the autotrophy culture of chlorella,
Shunting, Heterotrophic culture are automatically brought into operation to dehydration separation, and low to manpower degree of dependence, high degree of automation, cultivation cycle is short,
The yield and quality of chlorella is high.
It five, is provided with secondary nutrition mixed organization on the pipeline in the present invention between grower and proliferation device, it is secondary
Grade nutrition mixed organization includes secondary blending tank and secondary supplement tank, and secondary supplement tank is individually connected to secondary blending tank, secondary
Nutrition mixed organization can be to each proliferation tank of the chlorella group that distributes in being dispersed to proliferation device from growth slot
In before, carry out small-scale nutrient solution mixing and supplement in advance so that chlorella can adapt to Heterotrophic culture environment in advance, adjustment
The oneself state of chlorella so that chlorella can adapt to heterotrophism environment faster.Growth rate is promoted, to promote chlorella
Yield and quality, the secondly setting of secondary nutrition mixed organization can be convenient for holding the mixed culture concentration of chlorella so that
The chlorella quality and quantity being put into each proliferation tank is balanced, and the chlorella quality balance uniformly turned out also allows for uniting
One management.
Six, proliferation device includes several proliferation tanks in the present invention, each proliferation tank is both provided with independent pan feeding
The pipeline of valve is proliferated and is respectively provided between feedway and the proliferation tank of each with the independent pipeline growth slot for supporting valve, point
It is all connected with from tank and all proliferation tanks, such independent tubes design enables to proliferation tank all in proliferation device to have
There are independent charging, discharging to also have culture environment, can be used alone, can also use in batches in practical operation, it can be most
Big degree rationally utilizes device, while ensureing to produce, saves the operation energy consumption of device.
Above description is only the general introduction of technical solution of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, below with presently preferred embodiments of the present invention and after coordinating attached drawing to be described in detail such as.
The specific implementation mode of the present invention is shown in detail by following embodiment and its attached drawing.
Description of the drawings
Technical solution in technology in order to illustrate the embodiments of the present invention more clearly, in being described below to embodiment technology
Required attached drawing is briefly described, it should be apparent that, the accompanying drawings in the following description is only some realities of the present invention
Example is applied, it for those of ordinary skill in the art, without creative efforts, can also be according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the structure chart of the preferred embodiment of the present invention；
Fig. 2 is the structure chart of preferred embodiment of the present invention grower；
Fig. 3 is the structure chart of preferred embodiment of the present invention proliferation device；
Fig. 4 is the structure chart of preferred embodiment of the present invention separating circulating device
In Fig. 1-4：1, grower, 2, proliferation device, 3, proliferation feedway, 4, separating circulating device, 5, growth slot,
6, groove top means for screening, 7, increase stomata, 8, proliferation tank, 9, pan feeding valve, 10, nutriment blending tank, 11, nutrition tank, 12, NaOH solution tank NaOH,
13, valve is supported, 14, knockout drum, 15, circulating line, the 16, first illumination mechanism, the 17, second illumination mechanism, 18, secondary mixing
Tank, 19, secondary supplement tank, 20, circulating valve, 21, centrifugation control valve.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without creative efforts
Embodiment shall fall within the protection scope of the present invention.
Embodiment
As shown in Figs 1-4, a kind of circulating chlorella proliferation seperator is disclosed in the present embodiment, including grower 1,
Proliferation device 2, proliferation feedway 3 and separating circulating device 4, it is characterised in that：Grower 1 includes growth slot 5 and groove top
Means for screening 6,5 top of growth slot are open, and the setting of groove top means for screening 6 grows the interior groove of slot 5 on 5 top of growth slot
It is evenly arranged on wall and increases stomata 7；It is connected to by pipeline between proliferation device 2 and grower 1, proliferation device 2 includes several
A proliferation tank 8, each proliferation tank 8 are both provided with the pipeline with independent pan feeding valve 9；Feedway 3 is proliferated to mix including nutriment
Tank 10, nutrition tank 11, NaOH solution tank NaOH 12, are both provided with individual pipe between nutriment blending tank 10 and nutrition tank 11 and NaOH solution tank NaOH 12
Road is proliferated and is respectively provided between feedway 3 and the proliferation tank 8 of each with the independent pipeline growth slot 5 for supporting valve 13；Separation
Circulator 4 includes knockout drum 14 and circulating line 15, and knockout drum 14 is all connected with all proliferation tanks 8, and circulating line 15 connects
Logical knockout drum 14 and growth slot 5.Grower 1 and proliferation device 2 are provided in the present invention, chlorella can be first in grower 1
Middle and upper part is set as in open growth slot 5 carrying out photosynthesis using illumination carrying out autotrophy, is promoted and is sought in chlorella itself
It forms point and the content of protein, can also promote the population quality of chlorella itself, obtain the chlorella group of high quality, later
Chlorella group is dispersed in each proliferation tank 8 in proliferation device 2, and light is isolated after being mixed into organic carbon source, makes full use of
Heterotrophism carries out fast breeder, contains in this way in conjunction with the oneself protein matter and nutriment of the chlorella that autotrophy and heterotrophism mode are turned out
Amount is high, and population augment speed is fast, and yield is high, while repeatedly after Cyclic culture, and chlorella offspring degeneration is slow, can be made with persistent loop
With.By nutriment and solution separation supply, the shelf-life of mixed nutrient solution can be extended, miscellaneous bacteria therein is avoided to absorb
Nutritional ingredient influences the growth of chlorella, while feed separation, convenient for allotment and supplement, need not configure repeatedly, device according to
The usage quantity and actual needs for being proliferated tank 8 are supplemented, high to the utilization rate of raw material, save management manpower.Separation cycle dress
It includes knockout drum 14 and circulating line 15 to set 4, and knockout drum 14 can carry out dehydration separation to the chlorella being mingled in culture solution, directly
It connects to separate culture solution and retrogradation elongated slot 5 is then supplemented by circulating line 15, it is high to the cyclic utilization rate of material, after separation
Chlorella be convenient for rear road process operation.Grower 1 and proliferation device 2, proliferation feedway 3 and separating circulating device 4 it
Between setting be connected with pipeline, the autotrophy culture of chlorella, shunting, Heterotrophic culture are detached to dehydration and are automatically brought into operation, to manpower
Degree of dependence is low, and high degree of automation, cultivation cycle is short, and the yield and quality of chlorella is high.Grower 1 and proliferation device 2
Between pipeline on be provided with secondary nutrition mixed organization, secondary nutrition mixed organization includes secondary blending tank 18 and secondary supplement
Tank 19, secondary supplement tank 19 are individually connected to secondary blending tank 18, and secondary nutrition mixed organization can be to distributing from growth slot 5
Chlorella group in each proliferation tank 8 in being dispersed to proliferation device 2 before, carry out small-scale nutrient solution mixing in advance
And supplement so that chlorella can adapt to Heterotrophic culture environment in advance, adjust the oneself state of chlorella so that chlorella can be with
Heterotrophism environment is adapted to faster.Growth rate is promoted, to promote the yield and quality of chlorella, next secondary nutrition mixing machine
The setting of structure can be convenient for holding the mixed culture concentration of chlorella so that the chlorella quantity being put into each proliferation tank 8
And quality balance, the chlorella quality balance uniformly turned out also allow for being managed collectively.Proliferation device 2 is proliferated including several
Tank 8, each proliferation tank 8 are both provided with the pipeline with independent pan feeding valve 9, proliferation feedway 3 and the proliferation tank 8 of each it
Between be respectively provided with the independent pipeline growth slot 5 for supporting valve 13, knockout drum 14 is all connected with all proliferation tanks 8, such only
Vertical pipe design enables to proliferation tank 8 all in proliferation device 2 to all have independent charging, discharging also culture environment,
It can be used alone, can also use in batches in practical operation, can rationally utilize device to the greatest extent, ensureing to produce
While, save the operation energy consumption of device.Increase the air that stomata 7 can be sufficient to the chlorella supplement in growth slot 5, promotes bead
The photosynthesis effect of algae, at the same increase the air-flow emerged in stomata 7 can promote grow slot 5 in solution between exchange, promote
The distribution of chlorella so that the chlorella in slot can obtain sufficient autotrophic condition.
In the use of the present invention, the middle position in growth slot 5 is provided with the first illumination mechanism 16.First illumination mechanism
16 can carry out light supplement to the chlorella inside growth slot 5, promote the cultivation density of chlorella, moreover it is possible to promote the light of chlorella
Close function and effect.
In the use of the present invention, the side of groove top means for screening 6 towards growth slot 5 is connected with the second illumination mechanism 17.The
Two illumination mechanisms 17 can assist daylight or carry out light benefit to the chlorella for growing 5 otch of slot under conditions of no illumination
It fills, promotes the cultivation density of chlorella, moreover it is possible to promote the photosynthesis effect of chlorella.
In the use of the present invention, the notch that 5 top of growth slot opens up, groove top masking can be completely covered in groove top means for screening 6
Dimming glass layer is provided in mechanism 6.In the case where extraneous intensity of illumination is too strong, the light transmittance of dimming glass layer is adjusted, is protected
Card chlorella can obtain best intensity of illumination.
In the use of the present invention, being provided with secondary nutrition mixing machine on pipeline between grower 1 and proliferation device 2
Structure, secondary nutrition mixed organization includes secondary blending tank 18 and secondary supplements tank 19, secondary supplement tank 19 and secondary blending tank 18
Individually connection.Secondary nutrition mixed organization can be dispersed to proliferation device 2 to the chlorella group distributed from growth slot 5
In each proliferation tank 8 in before, carry out small-scale nutrient solution mixing and supplement in advance so that chlorella can adapt to different in advance
Culture environment is supported, adjusts the oneself state of chlorella so that chlorella can adapt to heterotrophism environment faster.Promote proliferation speed
Degree, to promote the yield and quality of chlorella, the secondly setting of secondary nutrition mixed organization can be convenient for holding chlorella
It is mixed concentration so that the chlorella quality and quantity being put into each proliferation tank 8 is balanced, the chlorella uniformly turned out
Quality balance also allows for being managed collectively.
In the use of the present invention, being provided with equalized temperature mechanism and ph balancing mechanism in proliferation tank 8.Equalized temperature machine
Structure can maintain the temperature in proliferation tank 8, ensure that chlorella has best growth temperature, ph balancing mechanism can be to increasing always
The acid-base value grown in tank 8 is adjusted so that the acid-base value in proliferation tank 8 maintains ideal set value always, ensures chlorella
Always there is best growth acid-base value.
In the use of the present invention, proliferation tank 8 is whole opaque.Light is avoided to enter proliferation tank 8 so that chlorella carries out light
Cooperation is used, and is proliferated without heterotrophism, is influenced the yield of chlorella, lighttight proliferation tank 8 can guarantee chlorella in tank into
Row heterotrophism, to ensure the yield of device.
In the use of the present invention, centrifugal mechanism is provided in knockout drum 14, on the pipeline between knockout drum 14 and proliferation tank 8
It is provided with centrifugation control valve 21, circulating line 15 is provided with circulating valve on the pipeline between connection knockout drum 14 and growth slot 5
20.So that centrifugal mechanism when carrying out centrifugal treating to chlorella, is isolated from the outside inside device, ensure centrifugation to the greatest extent
It is dehydrated separating effect.
In the use of the present invention, being additionally provided with drying mechanism in centrifugal mechanism.Drying mechanism can be to small after centrifugation
Ball algae carries out drying and processing, reduces band water, is convenient for subsequent operation.
The foregoing description of the disclosed embodiments enables those skilled in the art to implement or use the present invention.
Various modifications to these embodiments will be apparent to those skilled in the art, as defined herein
General Principle can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore, of the invention
It is not intended to be limited to the embodiments shown herein, and is to fit to and the principles and novel features disclosed herein phase one
The widest range caused.
Claims (10)
1. a kind of circulating chlorella is proliferated seperator, including grower, proliferation device, proliferation feedway and separation cycle
Device, it is characterised in that：The grower includes growth slot and groove top means for screening, the growth slot top be it is open,
The groove top means for screening setting is evenly arranged with increasing stomata on the growth slot top, the inside cell wall of the growth slot；
It being connected to by pipeline between the proliferation device and the grower, the proliferation device includes several proliferation tanks,
Each described proliferation tank is both provided with the pipeline with independent pan feeding valve；
The proliferation feedway includes nutriment blending tank, nutrition tank, NaOH solution tank NaOH, the nutriment blending tank and the nutrition tank and
It is both provided with individual pipeline between the NaOH solution tank NaOH, is all provided between the proliferation feedway and proliferation tank described in each
It sets with the independent pipeline for supporting valve；
The separating circulating device includes knockout drum and circulating line, and the knockout drum is all connected with all proliferation tanks,
The circulating line is connected to the knockout drum and the growth slot.
2. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：In the growth slot
Middle position is provided with the first illumination mechanism.
3. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：The groove top covers machine
The side of structure towards the growth slot is connected with the second illumination mechanism.
4. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：The groove top covers machine
The notch that the growth slot top opens up can be completely covered in structure, and dimming glass layer is provided in the groove top means for screening.
5. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：The grower and
Secondary nutrition mixed organization is provided on pipeline between the proliferation device, the secondary nutrition mixed organization includes secondary mixed
Tank and secondary supplement tank are closed, the secondary supplement tank is individually connected to the secondary blending tank.
6. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：It is set in the proliferation tank
It is equipped with equalized temperature mechanism and ph balancing mechanism.
7. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：The proliferation tank is whole
It is opaque.
8. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：It is set in the knockout drum
It is equipped with centrifugal mechanism, centrifugation control valve is provided on the pipeline between the knockout drum and the proliferation tank.
9. a kind of circulating chlorella according to claim 1 is proliferated seperator, it is characterised in that：The circulating line exists
It connects and is provided with circulating valve on the pipeline between the knockout drum and the growth slot.
10. a kind of circulating chlorella according to claim 8 is proliferated seperator, it is characterised in that：The centrifugal mechanism
In be additionally provided with drying mechanism.
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| CN103627620A (en) * | 2012-08-28 | 2014-03-12 | 西门子公司 | Method and apparatus for producing lipids through microalgae culture |
| CN204625611U (en) * | 2015-05-08 | 2015-09-09 | 天津农学院 | A kind of chlorella culture device |
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| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
| US20090298159A1 (en) * | 2008-05-27 | 2009-12-03 | Tsinghua University | Method for producing biodiesel from an alga |
| CN102089434A (en) * | 2008-07-30 | 2011-06-08 | 华盛顿州立大学研究基金会 | Integrated system for productioin of biofuel feedstock |
| US20110027827A1 (en) * | 2009-07-30 | 2011-02-03 | Zhanyou Chi | Integrated system for production of biofuel feedstock |
| CN103627620A (en) * | 2012-08-28 | 2014-03-12 | 西门子公司 | Method and apparatus for producing lipids through microalgae culture |
| CN204625611U (en) * | 2015-05-08 | 2015-09-09 | 天津农学院 | A kind of chlorella culture device |
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