CN108633844A - A method of assessing Karst ecosystem revegetation potentiality using soil animal - Google Patents
A method of assessing Karst ecosystem revegetation potentiality using soil animal Download PDFInfo
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- CN108633844A CN108633844A CN201810383049.8A CN201810383049A CN108633844A CN 108633844 A CN108633844 A CN 108633844A CN 201810383049 A CN201810383049 A CN 201810383049A CN 108633844 A CN108633844 A CN 108633844A
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- 239000002689 soil Substances 0.000 title claims abstract description 78
- 238000000034 method Methods 0.000 title claims abstract description 21
- 230000000284 resting effect Effects 0.000 claims abstract description 15
- 238000003556 assay Methods 0.000 claims abstract description 9
- 238000011084 recovery Methods 0.000 claims abstract description 8
- 239000011435 rock Substances 0.000 claims abstract description 8
- 238000009792 diffusion process Methods 0.000 claims abstract description 7
- 238000012360 testing method Methods 0.000 claims abstract description 7
- 238000002474 experimental method Methods 0.000 claims abstract description 6
- 239000000523 sample Substances 0.000 claims description 44
- 239000012805 animal sample Substances 0.000 claims description 8
- 238000012545 processing Methods 0.000 claims description 8
- 238000000926 separation method Methods 0.000 claims description 8
- 238000011065 in-situ storage Methods 0.000 claims description 5
- 230000012447 hatching Effects 0.000 claims description 4
- 238000005259 measurement Methods 0.000 claims description 4
- 230000035939 shock Effects 0.000 claims description 4
- 239000002344 surface layer Substances 0.000 claims description 4
- 230000015572 biosynthetic process Effects 0.000 claims description 2
- 238000003786 synthesis reaction Methods 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 5
- 238000004458 analytical method Methods 0.000 abstract description 2
- 102000002322 Egg Proteins Human genes 0.000 abstract 1
- 108010000912 Egg Proteins Proteins 0.000 abstract 1
- 210000004681 ovum Anatomy 0.000 abstract 1
- 238000011160 research Methods 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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Abstract
The invention belongs to Ecosystem Technologies fields, disclose a kind of method for assessing Karst ecosystem revegetation potentiality using soil animal, the assay method of the Karst ecosystem revegetation potentiality is using soil animal as object；Pass through culture experiment in field original position earth pillar Transplanting Test and soil animal ovum library room, the diffusion revegetation potentiality and resting egg library revegetation potentiality of overall merit karst soil animal.The present invention investigates and analyses soil animal diffusion revegetation potentiality and its influence factor under Karst Area of Guizhou difference Rock Desertification Degree, verifies soil animal resting egg library revegetation potentiality under Karst Rocky Desertification background by taking Karst Rocky Desertification type area Puding County Guizhou Province as an example.To the recovery for Karst Rocky Desertification Region Vulnerable Ecosystem scientific basis is provided with integrated control.
Description
Technical field
The invention belongs to Ecosystem Technologies fields more particularly to a kind of utilization soil animal to assess Karst ecosystem
The method of revegetation potentiality.
Background technology
Karst ecosystem is a kind of ecosystem of fragility, and because of mankind's unreasonable development, large area is degenerated,
Seriously threaten agriculture production environment or even human survival.The revegetation potentiality of degeneration Karst ecosystem is assessed to carrying out karst
The recovery of the ecosystem and reconstruction are significant.Existing evaluation measures are based primarily upon the recovery situation of aboveground vegetation, but
Since plant growing cycle is long, and related work can only be carried out in situ in the wild, thus time-consuming, and manpower, financial resources input are big.Soil
Earth animal is the important component of bio-diversity, and species quantity is more, density is big, distribution is wide, short (the exhausted big portion of life cycle
Divide species about 30 days), it is numerous convenient for indoor culture and expansion, thus be the effective object for assessing ecosystem restoration potentiality.
In conclusion problem of the existing technology is：Plant gives birth in the means of existing assessment Karst ecosystem
Long period is long, and time-consuming for assessment；Under the conditions of regional environment, soil animal is most suitable for the acquisition of living condition.
Invention content
In view of the problems of the existing technology, the present invention provides a kind of Karst ecosystem is assessed using soil animal
The method of revegetation potentiality.
The invention is realized in this way a method of Karst ecosystem revegetation potentiality being assessed using soil animal,
The method is using soil animal as object；By cultivating examination in field original position earth pillar Transplanting Test and soil animal resting egg library room
It tests, the diffusion revegetation potentiality and resting egg library revegetation potentiality of overall merit karst soil animal are various from terrestrial life
Property angle, the recovery to give birth to fragile state system for karst provides foundation with integrated control.
Further, the method for assessing Karst ecosystem revegetation potentiality using soil animal includes the following steps：
Soil animal spreads the assay method of revegetation potentiality：With choosing the sample of 4 kinds of different Rock Desertification Degrees；In each sample
The pedotheque on 10 centimetres of surface layer is acquired, 1 part of removal soil fauna, 1 part is control；By 20 mesh holes of each pedotheque
The mesh bag of diameter installs, and makes a diameter of 13 centimetres of earth pillar, samples original place with being put back into corresponding sample respectively, is repeated 7 times in sample prescription；
The 15th day after transplanting, 30 days and 60 days sample respectively, the soil animal sample that separation, identification obtain；It is carried out at the same time soil
The measurement of physicochemical property；
The assay method of soil animal resting egg library revegetation potentiality：To the without stony desertification sample native of removal soil animal live body
20 DEG C, 23 DEG C, 25 DEG C, 27 DEG C and 30 DEG C and 20%, 25% and 30% soil moisture is respectively set in earth sample, carries out indoor
The optimal culture condition of soil animal suspend mode egg hatching is explored in culture；The topsoil on 4 kinds of sample ground is gone through cold shock method at adult
After reason, under the optimum temperature and damp condition filtered out, sampled after culture being protected from light in incubator 30 days；Separation, identification obtain
Soil animal sample；Each processing is repeated 12 times.
Further, the sample for choosing 4 kinds of different Rock Desertification Degrees for no stony desertification R0, potential stony desertification R1, slightly
Stony desertification R2 and moderate stony desertification R3.
Further, the original place that samples with being put back into corresponding sample respectively will remove fauna and control without stony desertification sample
Ground soil in-situ with being transplanted to no stony desertification sample, is denoted as OR0 and WR0 and OR1, WR1, OR2, WR2, OR3 and WR3 is 8 kinds total
Processing.
Advantages of the present invention and good effect are：Guizhou is the area that China's karst landform is most developed, and belongs to typical
Ecologically fragile areas.This area's bio-diversity has extremely important in maintaining and improving ecosystem stability and its ecological functions
Value.The present invention investigates and analyses soil animal diffusion under Karst Area of Guizhou difference Rock Desertification Degree and restores by taking Puding County as an example
Potentiality verify soil animal resting egg library revegetation potentiality under Karst Rocky Desertification background.To for Karst Rocky Desertification Region it is crisp
The recovery of the weak ecosystem provides scientific basis with integrated control.It can be completed, can be improved existing in entire two months test periods
There is 50% or more evaluation measures efficiency.
Description of the drawings
Fig. 1 is the method stream provided in an embodiment of the present invention that Karst ecosystem revegetation potentiality is assessed using soil animal
Cheng Tu.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The present invention is using soil animal as research object, the research of thrust zone bio-diversity；Pass through field original position earth pillar
The diffusion of culture experiment in Transplanting Test and soil animal resting egg library room, overall merit karst soil animal restores latent
Power and resting egg library revegetation potentiality, from the multifarious angle of terrestrial life, to given birth to for karst the recovery of fragile state system with
Integrated control provides scientific basis.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in Figure 1, provided in an embodiment of the present invention assess Karst ecosystem revegetation potentiality using soil animal
Method includes the following steps：
S101：Using soil animal as object；By in field original position earth pillar Transplanting Test and soil animal resting egg library room
Culture experiment, the diffusion revegetation potentiality and resting egg library revegetation potentiality of overall merit karst soil animal are obtained and are degenerated
The suitable environment condition that ecosystem Soil Biodiversity restores；
S102：The angle restored from terrestrial life diversity, to give birth to recovery and the synthesis of fragile state system for karst
Prevention provides scientific basis.
The method provided in an embodiment of the present invention for assessing Karst ecosystem revegetation potentiality using soil animal is specifically wrapped
Include following steps：
Soil animal spreads the assay method of revegetation potentiality：With choosing the sample of 4 kinds of different Rock Desertification Degrees (no stony desertification
R0, potential stony desertification R1, slight stony desertification R2 and moderate stony desertification R3).In each sample acquire the soil on about 10 centimetres of surface layer
Sample, 1 part of removal soil fauna (O), 1 part is control (W)；Each pedotheque is installed with the mesh bag in 20 mesh apertures,
A diameter of 13 centimetres of earth pillar is made, samples original place with being put back into corresponding sample respectively (that is, the nothing that fauna and control will be removed
Stony desertification sample soil in-situ with being transplanted to no stony desertification sample, be denoted as OR0 and WR0 and OR1, WR1, OR2, WR2, OR3 and
The total 8 kinds of processing of WR3), it is repeated 7 times in sample prescription；The 15th day after transplanting, 30 days and 60 days sample respectively, and separation, identification obtain
The soil animal sample obtained；It is carried out at the same time the measurement of soil physico-chemical property；
The assay method of soil animal resting egg library revegetation potentiality：To the without stony desertification sample native of removal soil animal live body
20 DEG C, 23 DEG C, 25 DEG C, 27 DEG C and 30 DEG C and 20%, 25% and 30% soil moisture is respectively set in earth sample, carries out indoor
The optimal culture condition of soil animal suspend mode egg hatching is explored in culture；The topsoil on 4 kinds of sample ground is gone through cold shock method at adult
After reason, under the optimum temperature and damp condition filtered out, after culture being protected from light in incubator 30 days, sampling；Separation, identification obtain
Soil animal sample；Each processing is repeated 12 times.
The application principle of the present invention is further described with reference to experiment.
Experiment is carried out at Guizhou Province Puding Karst ecosystem observational study station.Consider vegetation, land use class
The factors such as type, the sample of 4 kinds of representative different Rock Desertification Degrees of Selecting research area：Without stony desertification (R0), potential stony desert
Change (R1), slight stony desertification (R2) and moderate stony desertification (R3) sample, the sample prescription of 3 50m*50m is selected to each sample, and fixed
Position, protection.
Soil animal spreads revegetation potentiality assay method
4 types of selection sample take the pedotheque on about 10 centimetres of surface layer, 1 part of removal soil fauna (O ,-
25/25 DEG C, 12/12h, circulating freezing resistance 3 times), 1 part is control (W, without any processing).Then by each pedotheque with 20
The mesh bag in mesh aperture installs, and makes a diameter of 13 centimetres of earth pillar, samples original place with being put back into corresponding sample respectively.That is, will removal
Fauna and and control without stony desertification sample soil move it is in situ be denoted as OR0 and WR0 with being transplanted to no stony desertification sample, and
Total 8 kinds of OR1, WR1, OR2, WR2, OR3 and WR3 is handled, and is repeated 7 times in sample prescription.Respectively the 15th day after transplanting, 30 days and
It samples within 60 days, the soil animal sample that separation, identification obtain.It is carried out at the same time the measurement of soil physico-chemical property.
Soil animal resting egg library revegetation potentiality assay method
To removing without stony desertification sample the pedotheque of soil animal live body, it is respectively set 20 DEG C, 23 DEG C, 25 DEG C, 27 DEG C
With 30 DEG C and 20%, 25% and 30% soil moisture (water content), indoor culture is carried out, soil animal suspend mode egg hatching is explored
Optimal culture condition.By the topsoil on 4 kinds of sample ground after cold shock method goes adult to handle, in the Optimal Temperature that filters out and wet
After being protected from light culture under the conditions of degree, in incubator 30 days, sampling.The soil animal sample that separation, identification obtain.Each processing repeats
12 times.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement etc., should all be included in the protection scope of the present invention made by within refreshing and principle.
Claims (4)
1. it is a kind of using soil animal assess Karst ecosystem revegetation potentiality method, which is characterized in that the method with
Soil animal is object；By culture experiment in field original position earth pillar Transplanting Test and soil animal resting egg library room, synthesis is commented
The diffusion revegetation potentiality and resting egg library revegetation potentiality of valence karst soil animal, from the multifarious angle of terrestrial life,
Recovery to give birth to fragile state system for karst provides foundation with integrated control.
2. as described in claim 1 using the method for soil animal assessment Karst ecosystem revegetation potentiality, feature exists
In the method for assessing Karst ecosystem revegetation potentiality using soil animal includes the following steps：
Soil animal spreads the assay method of revegetation potentiality：With choosing the sample of 4 kinds of different Rock Desertification Degrees；In each sample acquire
The pedotheque that 10 centimetres of surface layer, 1 part of removal soil fauna, 1 part is control；By 20 mesh apertures of each pedotheque
Mesh bag installs, and makes a diameter of 13 centimetres of earth pillar, samples original place with being put back into corresponding sample respectively, is repeated 7 times in sample prescription；Respectively
It samples within the 15th day, 30 days and 60 days after transplanting, the soil animal sample that separation, identification obtain；It is carried out at the same time soil physical chemistry
The measurement of property；
The assay method of soil animal resting egg library revegetation potentiality：To removing without stony desertification sample the soil-like of soil animal live body
20 DEG C, 23 DEG C, 25 DEG C, 27 DEG C and 30 DEG C and 20%, 25% and 30% soil moisture is respectively set in product, carries out indoor culture,
Explore the optimal culture condition of soil animal suspend mode egg hatching；By the topsoil on 4 kinds of sample ground after cold shock method goes adult to handle,
Under the optimum temperature and damp condition filtered out, sampled after culture being protected from light in incubator 30 days；The soil that separation, identification obtain
Animal sample；Each processing is repeated 12 times.
3. as claimed in claim 2 using the method for soil animal assessment Karst ecosystem revegetation potentiality, feature exists
Be in, the sample for choosing 4 kinds of different Rock Desertification Degrees no stony desertification R0, potential stony desertification R1, slight stony desertification R2 and in
Spend stony desertification R3.
4. as claimed in claim 2 using the method for soil animal assessment Karst ecosystem revegetation potentiality, feature exists
In without stony desertification sample the soil in-situ for removing fauna and control is moved in the original place that samples with being put back into corresponding sample respectively
With planting no stony desertification sample, OR0 and WR0 and the total 8 kinds of processing of OR1, WR1, OR2, WR2, OR3 and WR3 are denoted as.
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| CN201810383049.8A CN108633844B (en) | 2018-04-26 | 2018-04-26 | Method for evaluating recovery potential of karst ecosystem by utilizing soil animals |
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Cited By (3)
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| CN112365137A (en) * | 2020-10-28 | 2021-02-12 | 贵州大学 | Method for measuring karst habitat level heterogeneity based on microbial environment types |
| CN112425559A (en) * | 2020-11-05 | 2021-03-02 | 宁波大学 | Digital traceless accurate space sampling reset method for field soil animal collection |
| CN113740511A (en) * | 2021-09-06 | 2021-12-03 | 宁波大学 | Method for obtaining soil animal activity biosensor for farmland soil health diagnosis |
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