CN108575899A - A kind of cultural method improving crocodile tortoise nutritive value - Google Patents
A kind of cultural method improving crocodile tortoise nutritive value Download PDFInfo
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- CN108575899A CN108575899A CN201810499417.5A CN201810499417A CN108575899A CN 108575899 A CN108575899 A CN 108575899A CN 201810499417 A CN201810499417 A CN 201810499417A CN 108575899 A CN108575899 A CN 108575899A
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Sustainable Development (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Birds (AREA)
- Marine Sciences & Fisheries (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of cultural methods improving crocodile tortoise nutritive value, using the high feed feeding crocodile tortoise of a kind of ecological, environmental protective, nutritional ingredient and the management method of cooperation science, it can play and improve crocodile tortoise disease resistance, increase production chemokine, promote the growth of crocodile tortoise, accumulation of the nutriment in crocodile tortoise body is improved, and crocodile tortoise meat can be made more delicious and containing abundant organic selemium nutrition.Described plus selenium feed is made of herba hedyotis diffusae extract, freshwater shrimp powder, catfish powder, chicken organ powder, fermented bean dregs, expanded soybean, bone meal, carrot powder, watermelon peel powder, glucose, seawood meal, calcium dihydrogen phosphate, salt, crosslinking selenium methionine.
Description
Technical field
The present invention relates to aquaculture of aquatic animal technical fields, and in particular to a kind of cultivation side for improving crocodile tortoise nutritive value
Method.
Background technology
Crocodile tortoise, is existing most ancient reptile, one of the world's largest terrapin, the title for having fresh water animals king,
It is divided into two big types, is commonly called as big fish and small crocodile.Big fish also known as true crocodile tortoise (originating from North America southeastern US), small crocodile also known as quasi- crocodile
Tortoise, there are four subspecies, are North America, Florida State, South America, Sino-U.S. respectively.
Analysis of Nutritive Composition to crocodile tortoise muscle parts the result shows that, contain abundant moisture, sugar, fat, content of ashes.
Mineral element in crocodile tortoise muscle is with sodium highest, and carapace Mineral Elements are with calcium highest.Crocodile tortoise muscle fat content is 0.2%,
Contain abundant vitamin, a variety of amino acid needed by human in aliphatic acid.Crocodile tortoise is exactly that a kind of nutritive value is very high, and whole body is all
It is precious to support aquatic reptile.Its fine and tender taste, crisp-fried are delicious, belong to high protein, low fat, low in calories, low cholesterol,
The ticbit of lean meat species.
Selenium element is life kindling material, the five of anticancer, health this, can effectively prevent more than 40 kinds of human diseases.China has
72% regional selenium deficiency improves the Se content in food using addition Se-enriched feedstuff nutrition purposes, especially for feeding animals, is to improve humans and animals selemium nutrition
Essential measure.
With the raising that our people lives, requirement to diet and the attention of health is also improved, you can make delicious
Can play again food therapy health effect can cultivated animals be subject to the people's attention.But there are all for the artificial propagation of crocodile tortoise at present
More problems, including crocodile tortoise egg production is few or even does not lay eggs, reproductive efficiency is low, influences economic benefit；And in the hatching of egg
In the process, hatching success is low, and effect is poor；Cultural technique is more complicated, and growth is slower；It is improper to feed feed nutrition proportioning, leads
Cause nutrient composition content few.
Invention content
To solve the above-mentioned problems, the present invention provides a kind of cultural methods improving crocodile tortoise nutritive value, using one kind
The high feed feeding crocodile tortoise of ecological, environmental protective, nutritional ingredient and the management method for coordinating science, can play and improve the disease-resistant energy of crocodile tortoise
Power increases production chemokine, promotes the growth of crocodile tortoise, improves accumulation of the nutriment in crocodile tortoise body, and crocodile tortoise meat can be made more delicious
And contain abundant organic selemium nutrition.
The present invention can be achieved through the following technical solutions：
A kind of cultural method improving crocodile tortoise nutritive value, includes the following steps：
(1) it before putting in a suitable place to breed, is sterilized, is rinsed with clear water, young tortoise 20mg/L potassium permanganate with splash full pond of 100mg/L quick limes
Solution dipping bath；
(2) breeding density 40-60/m2, control 28-30 DEG C of water temperature, water pH7-8, transparency 30cm；
(3) young tortoise feeds 3 feeds daily, and day feeding volume is the 2-3% of crocodile tortoise weight, and adult tortoise feeds 2 feedings daily
Material, day feeding volume are the 5-8% of crocodile tortoise weight；
(4) spring and autumn changed water every 7 days 1 time, and summer changed water every 3 days 1 time, and quantity of exchanged water is the 20- of former water total amount
30%；
(5) per 15-20 days, aquaculture pond is per 667m3Put into 400-500 Pomacea canaliculata.
The feed is that selenium feed and immunomodulator will be added by 99:1 ratio mixing drying, then with water with 3:2
Ratio mixes, and 3% soya-bean oil is added, is put into granulator after stirring evenly, the pellet of diameter 6-8mm is made.
Described plus selenium feed：4.2-4.8 parts of herba hedyotis diffusae extract, 10-14 parts of freshwater shrimp powder, 21-23 parts of catfish powder, chicken
6-8 parts of visceral meal, 6-8 parts of fermented bean dregs, 3-5 parts of expanded soybean, 4.2-4.6 parts of bone meal, 2.2-2.4 parts of carrot powder, watermelon peel powder
1.1-1.3 parts, 0.7-0.9 parts of glucose, 1.2-1.4 parts of seawood meal, 1.4-1.8 parts of calcium dihydrogen phosphate, 0.2-0.4 parts of salt,
It is crosslinked 1.1-1.5 parts of selenium methionine.
The herba hedyotis diffusae extract：By fresh oldenlandia diffusa drying and crushing, solid-liquid ratio 1 is added:1.5 85% second
Alcohol merges extracting solution in 60 DEG C of refluxing extractions 2 times, each 92min, filters to take filtrate drying.
The crosslinking selenium methionine：Selenium methionine is pressed 1:5 ratio is scattered in 2% chitosan solution containing 2% acetic acid
In, it is then poured slowly into the atoleine of the span80 containing water phase 20%, stirring forms w/o type lotion, is added 4 times
Glutaraldehyde reacts 3-4h at 1000r/min, 25 DEG C, centrifuges 15min through 3000r/min, takes precipitation drying.
The immunomodulator：5-7 parts of vegetable active composite extract, 6-8 parts of Prunella vulgaris, 5-7 parts of radix scrophulariae, stem pith of the rice-paper plant 5-7
Part, 8-12 parts of jujube kernel, 3-5 parts of Poria cocos, 2-3 parts of cordate houttuynia, 5-7 parts of dried orange peel, 6-10 parts of Cortex Eucommiae, 11-17 parts of fennel, Radix Angelicae Sinensis 6-8
Part, 5-7 parts of hawthorn, 5-7 parts of the fruit of Rangoon creeper, 8-10 parts of Radix Glycyrrhizae；The vegetable active composite extract：Camellia powder extracts 12-18
Part, 2-4 parts of agrocybe extract.
The camellia powder extracts：Camellia pollen is taken, solid-liquid ratio 1 is added:5, pH4.5 citrate-phosphate disodium hydrogen buffering
15% cellulase is added by camellia pollen quality after mixing in liquid, after 55 DEG C digest 5h, centrifuges 10min through 4000r/min, takes
Supernatant is dried.
The agrocybe extract：It is 1 to take agrocybe powder that solid-liquid ratio is added:20 complex enzyme, at pH5.0,50 DEG C
2h is digested, the ethyl alcohol of 3 times of volumes is added after enzymolysis, is stood in 4 DEG C for 24 hours, is centrifuged 10min through 6000r/min, take precipitation drying；
The complex enzyme is cellulase：Zytase：1,4 beta-glucanase=3:3:6.
The vegetable active compound technique：Using sodium alginate as carrier, Ca2+For crosslinking agent, at 60 DEG C, pH8.0, sea
Mosanom a concentration of 2.50%, plant extracts volume：Sodium alginate volume=1:Extract is immobilized under conditions of 2
3h。
The present invention has following advantageous effect：
The present invention relates to a kind of cultural method improving crocodile tortoise nutritive value, herba hedyotis diffusae extract adds in feed
Enter, promote the crocodile tortoise speed of growth, improve food conversion ratio, reduces blood glucose, cholesterol, content of triglyceride and blood in crocodile tortoise body
Lipid peroxidation object amount in clear, improves the oxidation resistance and immunity of crocodile tortoise.
The crosslinking selenium methionine that the present invention is added using chitosan have hydrophily, good biocompatibility and can biology
The advantages that degradability, uses chitosan to convey selenium methionine as carrier, promotes the accumulation of selenium element content in crocodile tortoise body.
Vegetable active composite extract of the present invention is added in feed and is used cooperatively, and can extend the drug effect of Chinese herbal medicine in feed,
Improve the disease resistance of crocodile tortoise；Gastrointestinal tract pH value is reduced, inhibits to be harmful to pathogen, ensures crocodile tortoise healthy growth；Improve feed
Digestibility reduces aquaculture cost；The immunity and disease resistance of crocodile tortoise can also be improved, culture efficiency is improved；Immobilization
Vegetable active composite extract afterwards can sustainedly and stably maintain bioactivity, extend action time, promote the absorption of crocodile tortoise
It utilizes.
The Chinese medicinal herb nutrient value that immunological regulation agent prescription is added in the present invention is high, containing there are many amino acid, trace element, dimensions
Raw element and bioactive substance, can promote the synthesis of body glycometabolism, protein and enzyme.Enhance body antibody titer, irritation
The effects that developing, bactericidal, adjusting the immune function of body；Chinese herb feed additive has no toxic side effect, in animal body without residual
It stays and does not develop immunity to drugs；Have the double action of trophism and Drug concurrently；It can promote crocodile tortoise gonad development, ovulation, raising
Rate of fertilization has health care immunization.
The present invention feeds crocodile tortoise using Se-enriched feedstuff, improves the functionality such as polypeptide, amino acid and solubility calcium in crocodile tortoise
The content of substance completes the enrichment of Organic Selenium, promotes the growth and development of crocodile tortoise, improves its immunity and premunition.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of cultural method improving crocodile tortoise nutritive value, mainly includes the following steps that：
(1) it before putting in a suitable place to breed, is sterilized, is rinsed with clear water, young tortoise 20mg/L potassium permanganate with splash full pond of 100mg/L quick limes
Solution dipping bath；
(2) 50/m of breeding density2, control 29 DEG C, water pH=7.5, transparency 30cm of water temperature；
(3) young tortoise feeds 3 feeds daily, and day feeding volume is the 2.5% of crocodile tortoise weight, and adult tortoise feeds 2 feedings daily
Material, day feeding volume are the 6.5% of crocodile tortoise weight；
(4) spring and autumn changed water every 7 days 1 time, and summer changed water every 3 days 1 time, and quantity of exchanged water is the 25% of former water total amount；
(5) every 17.5 days, aquaculture pond was per 667m3Put into 450 Pomacea canaliculatas.
A kind of cultural method improving crocodile tortoise nutritive value of the present embodiment, the feed is will to add selenium feed and immunological regulation
Agent presses 99:1 ratio mixing drying, then with water with 3:2 ratio mixing, is added 3% soya-bean oil, particle is put into after stirring evenly
In machine, the pellet of diameter 7mm is made.
It is above-mentioned plus selenium feed to produce：4.5 parts of herba hedyotis diffusae extract, 12 parts of freshwater shrimp powder, 22 parts of catfish powder, chicken organ
7 parts of powder, 7 parts of fermented bean dregs, 4 parts of expanded soybean, 4.4 parts of bone meal, 2.3 parts of carrot powder, 1.2 parts of watermelon peel powder, 0.8 part of glucose,
1.3 parts 1.3 parts of seawood meal, 1.6 parts of calcium dihydrogen phosphate, 0.3 part of salt, crosslinking of selenium methionine.
Above-mentioned herba hedyotis diffusae extract is produced：By fresh oldenlandia diffusa drying and crushing, solid-liquid ratio 1 is added:1.5
85% ethyl alcohol merges extracting solution in 60 DEG C of refluxing extractions 2 times, each 92min, filters to take filtrate drying.
Above-mentioned crosslinking selenium methionine is produced：Selenium methionine is pressed 1:It is poly- that 5 ratio is scattered in 2% shell containing 2% acetic acid
It in sugar juice, is then poured slowly into the atoleine of the span80 containing water phase 20%, stirring forms w/o type lotion, adds
The glutaraldehyde for entering 4 times reacts 3.5h at 1000r/min, 25 DEG C, centrifuges 15min through 3000r/min, takes precipitation drying.
Above-mentioned immunomodulator is produced：6 parts of vegetable active composite extract, 7 parts of Prunella vulgaris, 6 parts of radix scrophulariae, 6 parts of the stem pith of the rice-paper plant,
10 parts of jujube kernel, 4 parts of Poria cocos, 2.5 parts of cordate houttuynia, 6 parts of dried orange peel, 8 parts of Cortex Eucommiae, 14 parts of fennel, 7 parts of Radix Angelicae Sinensis, 6 parts of hawthorn, the fruit of Rangoon creeper 6
Part, 9 parts of Radix Glycyrrhizae；The vegetable active composite extract：15 parts of camellia powder extracts, 3 parts of agrocybe extract.
The preparation of above-mentioned camellia powder extracts：Camellia pollen is taken, solid-liquid ratio 1 is added:5, pH=4.5 citrate-phosphate hydrogen
15% cellulase is added by camellia pollen quality after mixing in disodium buffer solution, after 55 DEG C digest 5h, is centrifuged through 4000r/min
10min takes supernatant to dry.
The preparation of above-mentioned agrocybe extract：It is 1 to take agrocybe powder that solid-liquid ratio is added:20 complex enzyme, in pH=
2h is digested at 5.0,50 DEG C, the ethyl alcohol of 3 times of volumes is added after enzymolysis, is stood in 4 DEG C for 24 hours, is centrifuged 10min through 6000r/min, take
Precipitate drying；The complex enzyme is cellulase:Zytase:1,4 beta-glucanase=3:3:6.
Above-mentioned vegetable active compound technique is：Using sodium alginate as carrier, Ca2+For crosslinking agent, at 60 DEG C, pH=8.0,
Sodium alginate concentration is 2.50%, plant extracts volume：Sodium alginate volume=1:Extract is fixed under conditions of 2
Change 3h.
Embodiment 2
A kind of cultural method improving crocodile tortoise nutritive value, mainly includes the following steps that：
(1) it before putting in a suitable place to breed, is sterilized, is rinsed with clear water, young tortoise 20mg/L potassium permanganate with splash full pond of 100mg/L quick limes
Solution dipping bath；
(2) 40/m of breeding density2, control 28 DEG C, water pH=7, transparency 30cm of water temperature；
(3) young tortoise feeds 3 feeds daily, and day feeding volume is the 2% of crocodile tortoise weight, and adult tortoise feeds 2 feeds daily,
Day feeding volume is the 5% of crocodile tortoise weight；
(4) spring and autumn changed water every 7 days 1 time, and summer changed water every 3 days 1 time, and quantity of exchanged water is the 20% of former water total amount；
(5) every 15 days, aquaculture pond was per 667m3Put into 400 Pomacea canaliculatas.
A kind of cultural method improving crocodile tortoise nutritive value of the present embodiment, the feed is will to add selenium feed and immunological regulation
Agent presses 99:1 ratio mixing drying, then with water with 3:2 ratio mixing, is added 3% soya-bean oil, particle is put into after stirring evenly
In machine, the pellet of diameter 6mm is made.
It is above-mentioned plus selenium feed to produce：4.2 parts of herba hedyotis diffusae extract, 10 parts of freshwater shrimp powder, 21 parts of catfish powder, chicken organ
6 parts of powder, 6 parts of fermented bean dregs, 3 parts of expanded soybean, 4.2 parts of bone meal, 2.2 parts of carrot powder, 1.1 parts of watermelon peel powder, 0.7 part of glucose,
1.1 parts 1.2 parts of seawood meal, 1.4 parts of calcium dihydrogen phosphate, 0.2 part of salt, crosslinking of selenium methionine.
Above-mentioned herba hedyotis diffusae extract is produced：By fresh oldenlandia diffusa drying and crushing, solid-liquid ratio 1 is added:1.5
85% ethyl alcohol merges extracting solution in 60 DEG C of refluxing extractions 2 times, each 92min, filters to take filtrate drying.
Above-mentioned crosslinking selenium methionine is produced：Selenium methionine is pressed 1:It is poly- that 5 ratio is scattered in 2% shell containing 2% acetic acid
It in sugar juice, is then poured slowly into the atoleine of the span80 containing water phase 20%, stirring forms w/o type lotion, adds
The glutaraldehyde for entering 4 times reacts 3h at 1000r/min, 25 DEG C, centrifuges 15min through 3000r/min, takes precipitation drying.
Above-mentioned immunomodulator is produced：5 parts of vegetable active composite extract, 6 parts of Prunella vulgaris, 5 parts of radix scrophulariae, 5 parts of the stem pith of the rice-paper plant,
8 parts of jujube kernel, 3 parts of Poria cocos, 2 parts of cordate houttuynia, 5 parts of dried orange peel, 6 parts of Cortex Eucommiae, 11 parts of fennel, 6 parts of Radix Angelicae Sinensis, 5 parts of hawthorn, 5 parts of the fruit of Rangoon creeper,
8 parts of Radix Glycyrrhizae；The vegetable active composite extract：12 parts of camellia powder extracts, 2 parts of agrocybe extract.
The preparation of above-mentioned camellia powder extracts：Camellia pollen is taken, solid-liquid ratio 1 is added:5, pH=4.5 citrate-phosphate hydrogen
15% cellulase is added by camellia pollen quality after mixing in disodium buffer solution, after 55 DEG C digest 5h, is centrifuged through 4000r/min
10min takes supernatant to dry.
The preparation of above-mentioned agrocybe extract：It is 1 to take agrocybe powder that solid-liquid ratio is added:20 complex enzyme, in pH=
2h is digested at 5.0,50 DEG C, the ethyl alcohol of 3 times of volumes is added after enzymolysis, is stood in 4 DEG C for 24 hours, is centrifuged 10min through 6000r/min, take
Precipitate drying；The complex enzyme is cellulase:Zytase:1,4 beta-glucanase=3:3:6.
Above-mentioned vegetable active compound technique is：Using sodium alginate as carrier, Ca2+For crosslinking agent, at 60 DEG C, pH=8.0,
Sodium alginate concentration is 2.50%, plant extracts volume：Sodium alginate volume=1:Extract is fixed under conditions of 2
Change 3h.
Embodiment 3
A kind of cultural method improving crocodile tortoise nutritive value, mainly includes the following steps that：
(1) it before putting in a suitable place to breed, is sterilized, is rinsed with clear water, young tortoise 20mg/L potassium permanganate with splash full pond of 100mg/L quick limes
Solution dipping bath；
(2) 60/m of breeding density2, control 30 DEG C, water pH=8, transparency 30cm of water temperature；
(3) young tortoise feeds 3 feeds daily, and day feeding volume is the 3% of crocodile tortoise weight, and adult tortoise feeds 2 feeds daily,
Day feeding volume is the 8% of crocodile tortoise weight；
(4) spring and autumn changed water every 7 days 1 time, and summer changed water every 3 days 1 time, and quantity of exchanged water is the 30% of former water total amount；
(5) every 20 days, aquaculture pond put into 500 Pomacea canaliculatas per 667m3.
A kind of cultural method improving crocodile tortoise nutritive value of the present embodiment, the feed is will to add selenium feed and immunological regulation
Agent presses 99:1 ratio mixing drying, then with water with 3:2 ratio mixing, is added 3% soya-bean oil, particle is put into after stirring evenly
In machine, the pellet of diameter 8mm is made.
It is above-mentioned plus selenium feed to produce：4.8 parts of herba hedyotis diffusae extract, 14 parts of freshwater shrimp powder, 23 parts of catfish powder, chicken organ
8 parts of powder, 8 parts of fermented bean dregs, 5 parts of expanded soybean, 4.6 parts of bone meal, 2.4 parts of carrot powder, 1.3 parts of watermelon peel powder, 0.9 part of glucose,
1.5 parts 1.4 parts of seawood meal, 1.8 parts of calcium dihydrogen phosphate, 0.4 part of salt, crosslinking of selenium methionine.
Above-mentioned herba hedyotis diffusae extract is produced：By fresh oldenlandia diffusa drying and crushing, solid-liquid ratio 1 is added:1.5
85% ethyl alcohol merges extracting solution in 60 DEG C of refluxing extractions 2 times, each 92min, filters to take filtrate drying.
Above-mentioned crosslinking selenium methionine is produced：Selenium methionine is pressed 1:It is poly- that 5 ratio is scattered in 2% shell containing 2% acetic acid
It in sugar juice, is then poured slowly into the atoleine of the span80 containing water phase 20%, stirring forms w/o type lotion, adds
The glutaraldehyde for entering 4 times reacts 4h at 1000r/min, 25 DEG C, centrifuges 15min through 3000r/min, takes precipitation drying.
Above-mentioned immunomodulator is produced：7 parts of vegetable active composite extract, 8 parts of Prunella vulgaris, 7 parts of radix scrophulariae, 7 parts of the stem pith of the rice-paper plant,
12 parts of jujube kernel, 5 parts of Poria cocos, 3 parts of cordate houttuynia, 7 parts of dried orange peel, 10 parts of Cortex Eucommiae, 17 parts of fennel, 8 parts of Radix Angelicae Sinensis, 7 parts of hawthorn, the fruit of Rangoon creeper 7
Part, 10 parts of Radix Glycyrrhizae；The vegetable active composite extract：18 parts of camellia powder extracts, 4 parts of agrocybe extract.
The preparation of above-mentioned camellia powder extracts：Camellia pollen is taken, solid-liquid ratio 1 is added:5, pH=4.5 citrate-phosphate hydrogen
15% cellulase is added by camellia pollen quality after mixing in disodium buffer solution, after 55 DEG C digest 5h, is centrifuged through 4000r/min
10min takes supernatant to dry.
The preparation of above-mentioned agrocybe extract：It is 1 to take agrocybe powder that solid-liquid ratio is added:20 complex enzyme, in pH=
2h is digested at 5.0,50 DEG C, the ethyl alcohol of 3 times of volumes is added after enzymolysis, is stood in 4 DEG C for 24 hours, is centrifuged 10min through 6000r/min, take
Precipitate drying；The complex enzyme is cellulase:Zytase:1,4 beta-glucanase=3:3:6.
Above-mentioned vegetable active compound technique is：Using sodium alginate as carrier, Ca2+For crosslinking agent, at 60 DEG C, pH=8.0,
Sodium alginate concentration is 2.50%, plant extracts volume：Sodium alginate volume=1:Extract is fixed under conditions of 2
Change 3h.
Several embodiments of the invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Cannot the limitation to the scope of the claims of the present invention therefore be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect range.
Claims (9)
1. a kind of cultural method improving crocodile tortoise nutritive value, which is characterized in that include the following steps：
(1) it before putting in a suitable place to breed, is sterilized, is rinsed with clear water, young tortoise 20mg/L liquor potassic permanganates with splash full pond of 100mg/L quick limes
Dipping bath；
(2) breeding density 40-60/m2, control 28-30 DEG C of water temperature, water pH7-8, transparency 30cm；
(3) young tortoise feeds 3 feeds daily, and day feeding volume is the 2-3% of crocodile tortoise weight, and adult tortoise feeds 2 feeds, day daily
Feeding volume is the 5-8% of crocodile tortoise weight；
(4) spring and autumn changed water every 7 days 1 time, and summer changed water every 3 days 1 time, and quantity of exchanged water is the 20-30% of former water total amount；
(5) per 15-20 days, aquaculture pond is per 667m3Put into 400-500 Pomacea canaliculata.
2. a kind of cultural method improving crocodile tortoise nutritive value according to claim 1, which is characterized in that the feed is
Selenium feed and immunomodulator will be added to press 99:1 ratio mixing drying, then with water with 3:2 ratio mixing, is added 3% beans
Oil is put into after stirring evenly in granulator, and the pellet of diameter 6-8mm is made.
3. a kind of cultural method improving crocodile tortoise nutritive value according to claim 2, which is characterized in that described plus selenium is raised
Material：4.2-4.8 parts of herba hedyotis diffusae extract, 10-14 parts of freshwater shrimp powder, 21-23 parts of catfish powder, 6-8 parts of chicken organ powder, fermentation beans
6-8 parts of the dregs of rice, 3-5 parts of expanded soybean, 4.2-4.6 parts of bone meal, 2.2-2.4 parts of carrot powder, 1.1-1.3 parts of watermelon peel powder, glucose
0.7-0.9 parts, 1.2-1.4 parts of seawood meal, 1.4-1.8 parts of calcium dihydrogen phosphate, 0.2-0.4 parts of salt, crosslinking selenium methionine 1.1-
1.5 part.
4. a kind of cultural method improving crocodile tortoise nutritive value according to claim 3, which is characterized in that the long-noded pit viper
Diffusa extract：By fresh oldenlandia diffusa drying and crushing, solid-liquid ratio 1 is added:1.5 85% ethyl alcohol, in 60 DEG C of refluxing extractions 2
Secondary, each 92min merges extracting solution, filters to take filtrate drying.
5. a kind of cultural method improving crocodile tortoise nutritive value according to claim 3, which is characterized in that the crosslinking egg
Propylhomoserin selenium：Selenium methionine is pressed 1:5 ratio is scattered in 2% chitosan solution containing 2% acetic acid, be then poured slowly into containing
In the atoleine for having the span80 of water phase 20%, stirring forms w/o type lotion, and 4 times of glutaraldehyde is added, in 1000r/min,
3-4h is reacted at 25 DEG C, 15min is centrifuged through 3000r/min, takes precipitation drying.
6. a kind of cultural method improving crocodile tortoise nutritive value according to claim 2, which is characterized in that the immune tune
Save agent：5-7 parts of vegetable active composite extract, 6-8 parts of Prunella vulgaris, 5-7 parts of radix scrophulariae, 5-7 parts of the stem pith of the rice-paper plant, 8-12 parts of jujube kernel, Poria cocos
3-5 parts, 2-3 parts of cordate houttuynia, 5-7 parts of dried orange peel, 6-10 parts of Cortex Eucommiae, 11-17 parts of fennel, 6-8 parts of Radix Angelicae Sinensis, 5-7 parts of hawthorn, the fruit of Rangoon creeper
5-7 parts, 8-10 parts of Radix Glycyrrhizae；The vegetable active composite extract：12-18 parts of camellia powder extracts, agrocybe extract 2-4
Part.
7. a kind of cultural method improving crocodile tortoise nutritive value according to claim 6, which is characterized in that the camellia pollen
Extract：Camellia pollen is taken, solid-liquid ratio 1 is added:5, pH4.5 citrate-phosphate disodium hydrogen buffer solution presses camellia silty after mixing
15% cellulase is added in amount, after 55 DEG C digest 5h, centrifuges 10min through 4000r/min, supernatant is taken to dry.
8. a kind of cultural method improving crocodile tortoise nutritive value according to claim 6, which is characterized in that the agrocybe
Extract：It is 1 to take agrocybe powder that solid-liquid ratio is added:20 complex enzyme digests 2h at pH5.0,50 DEG C, 3 is added after enzymolysis
The ethyl alcohol of times volume stands for 24 hours in 4 DEG C, centrifuges 10min through 6000r/min, take precipitation drying；The complex enzyme is cellulose
Enzyme：Zytase：1,4 beta-glucanase=3:3:6.
9. a kind of cultural method improving crocodile tortoise nutritive value according to claim 6, which is characterized in that the plant lives
Property compound technique：Using sodium alginate as carrier, Ca2+ is crosslinking agent, at 60 DEG C, pH8.0, sodium alginate concentration is
2.50%, plant extracts volume：Sodium alginate volume=1:3h is immobilized to extract under conditions of 2.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105174639A (en) * | 2015-10-08 | 2015-12-23 | 罗秋斌 | Method for removing antibiotics from turtle aquaculture wastewater |
| CN107637573A (en) * | 2017-10-18 | 2018-01-30 | 湖北聚注通用技术研究有限公司 | A kind of cultural method for improving soft-shelled turtle nourishing value |
| CN107927579A (en) * | 2017-12-22 | 2018-04-20 | 兴业县众旺种植专业合作社 | A kind of miscellaneous bacteria diet nutritional rice and preparation method thereof |
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2018
- 2018-05-23 CN CN201810499417.5A patent/CN108575899A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105174639A (en) * | 2015-10-08 | 2015-12-23 | 罗秋斌 | Method for removing antibiotics from turtle aquaculture wastewater |
| CN107637573A (en) * | 2017-10-18 | 2018-01-30 | 湖北聚注通用技术研究有限公司 | A kind of cultural method for improving soft-shelled turtle nourishing value |
| CN107927579A (en) * | 2017-12-22 | 2018-04-20 | 兴业县众旺种植专业合作社 | A kind of miscellaneous bacteria diet nutritional rice and preparation method thereof |
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Application publication date: 20180928 |