CN108420861B - Traditional Chinese medicine composition for preventing and treating poultry group I adenovirus and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition for preventing and treating poultry group I adenovirus and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of poultry breeding, in particular to a traditional Chinese medicine composition for preventing and treating poultry group I adenovirus, and a preparation method and application thereof. The traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is prepared from the following raw material components in parts by mass: 20-130 parts of salvia miltiorrhiza, 20-130 parts of hairyvein agrimony, 10-60 parts of humifuse euphorbia herb, 10-60 parts of poria cocos, 10-60 parts of red halloysite and 10-60 parts of liquorice; the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is simple in preparation method and convenient to use, can prevent and treat the poultry group I adenovirus, and can be directly drenched, drunk or mixed during the high disease period of poultry, so that the disease resistance of the poultry which is not attacked can be improved, and the disease incidence is reduced; for sick poultry, the treatment by drenching or freely drinking water can receive satisfactory effect, and the invention can obviously reduce the loss of the disease to farmers.
Description
Technical Field
The invention relates to the technical field of poultry breeding, in particular to a traditional Chinese medicine composition for preventing and treating poultry group I adenovirus, and a preparation method and application thereof.
Background
Group I avian adenoviruses are predominantly classical avian adenoviruses, and representative strains are avian embryo lethal orphan viruses (CELO), which share a common group-specific antigen of 12 serotypes isolated from chicken (FAV1-12), two serotypes of turkey (FAV1-2), three serotypes of goose (GAV1-3), and 2 serotypes of duck. The avian adenovirus group I mainly causes egg drop syndrome, inclusion body hepatitis, hydropericardium syndrome and the like of chickens. The avian adenovirus I exists in respiratory tract and digestive tract of various birds, is caused by primary pathogeny and is often mixed with other viruses such as CAV, IBDV and the like to cause diseases. The adenovirus pathogens causing the inclusion body hepatitis and the pericardial effusion of chickens are determined and are mostly caused by specific serotypes of the avian adenosis of group I or mixed infection of different serotypes. Viruses are found mainly in the eyes, upper respiratory tract and digestive tract of chickens. Viruses are mainly replicated in the digestive tract and upper respiratory tract, and feces, intestinal tracts, diseased livers, kidneys, pharynx and the like all contain viruses. The virus is primarily vertically transmitted through the chick embryos and can be exposed from cell isolates prepared from the chick embryos or chicks of the infected flock. The virus is generally discharged at the age of more than 3 weeks, and the peak of the virus discharge is 4-6 weeks in broiler chickens; 70% of laying hens can expel toxin after 5-9 weeks and 14 weeks old. Several different serotypes may exist simultaneously in the same farm. The virus can be horizontally transmitted through excrement, trachea and nasal mucosa, and sick chickens often have no obvious symptoms, but have the symptoms of collapse, crouch, fluffy feather, beard and pale face skin and poor growth. The autopsy is hepatitis, the liver is light and crisp, the liver is swollen, bleeding spots exist on the liver and skeletal muscles, and large and round or irregular basophilic or eosinophilic intranuclear inclusion bodies exist in liver cells. The inclusion body hepatitis mainly causes the death rate of chicken groups to be increased, the production performance is disturbed, the production is delayed and the development is poor, the severe anemia of sick chicken, the hepatomegaly, the hemorrhage and the necrosis are caused, and the adenogastromegaly or the ulcer is caused by the digestive system disorder. Particularly, since the outbreak of the serum 4 type which is characterized by pericardial hydrops in more provinces of China in 2015, serious economic loss is caused to the breeding industry. Serous type 4 causes pericardial effusion and liver and kidney injury, severe casualties occur, and the loss caused by production is huge. Clinically, sick chickens suddenly become depressed, loose feathers, diarrhea, anemia, emaciation and death. The autopsy finds that the liver is swollen, crisp and fragile, perihepatitis, pericardial effusion and jelly-like, the spleen has white dead spots, the trachea has bleeding and mucus, the lung has serious congestion, the glandular stomach has bleeding spots in individual cases, all the bursa of Fabricius has milk-white cheese-like substances, and the kidney has dendritic congestion. Wherein the virus can be transmitted by vertical transmission and horizontal transmission. Wherein the infection is transmitted to the next generation through respiratory tract, digestive tract and conjunctiva of eyes, and also through hatching eggs.
At present, the method for treating inclusion body hepatitis and hydropericardium of the sick chickens is relatively deficient, wherein the sick chickens are mainly injected with antibodies to assist symptomatic conservative treatment, and have certain value in clinic. However, when the flock is at peak morbidity, injection and stress can also lead to high mortality and possibly spread of disease through the injection needle. Second, antibody therapy needs to be targeted, and if infected chicken groups are not matched with the virus types and antibodies, namely the serotypes are not matched, the therapeutic effect is reduced or ineffective. And the labor intensity of injection administration is high, and the stress and the injury are also huge. Is not favorable for clinical operation. Secondly, after the disease occurs, the feed is added with compound vitamins and trace elements and a medicine for protecting the liver and the kidney and promoting diuresis is added, which has certain effect but limited effect.
Through the search of the prior art, the existing treatment modes aiming at the group I adenovirus, such as inclusion body hepatitis, hydropericardium, adenogastric enlargement and the like, are mainly antibodies, and then traditional Chinese medicines, western medicines or biological preparations, such as interferon and the like, have certain effects. However, the injection is labor-intensive and easy to contaminate and cause transmission, and serotype matching problems also exist. Most western medicines are common antiviral medicines such as ribavirin, however, the western medicines belong to medicines prohibited by the nation and can bring residues which are not beneficial to food safety. The application of the traditional Chinese medicine also has a chance.
Disclosure of Invention
In order to overcome the defects and shortcomings of the prior art, the invention aims at providing the traditional Chinese medicine composition for preventing and treating the I group adenovirus of poultry, which is convenient to use, has good effects of preventing and treating the I group adenovirus and can obviously reduce the loss of farmers caused by the disease.
The invention also aims to provide a preparation method of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, and the preparation method is simple.
The invention further aims to provide application of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus.
The purpose of the invention is realized by the following technical scheme:
a traditional Chinese medicine composition for preventing and treating I-group adenovirus of poultry is prepared from the following raw material components in parts by mass:
the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is preferably prepared from the following raw material components in parts by mass:
the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is preferably prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus comprises the following steps:
(1) mixing the raw material components of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus with water, decocting, filtering, and combining the filtrates;
(2) concentrating the filtrate prepared in the step (1) to obtain a concentrated solution; adjusting the pH value of the concentrated solution to 4.5-5.5 to obtain the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus;
the components of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus in the step (1) are preferably pretreated as follows: soaking and rinsing with water to remove impurities and dust;
the preferable times of decoction in the step (1) are 2-4 times, and each time lasts for 1.5-3 hours;
the decoction in the step (1) is preferably steam reflux decoction;
the specific operation of the steam reflux decoction is preferably as follows: mixing each raw material component of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus with water with the mass of 8-10 times, soaking for 8-10 hours, carrying out reflux decoction for 2-3 hours by using steam, and filtering to obtain filtrate and filter residue; mixing filter residues with 3-4 times of water by mass, and performing reflux decoction for 1.5-2 hours by using steam;
the specific gravity of the concentrated solution obtained in the step (2) is preferably 1.05-1.15;
the pH is preferably adjusted by hydrochloric acid with the mass fraction of 1%;
the preparation method of the traditional Chinese medicine composition for preventing and treating poultry group I adenovirus preferably further comprises the following steps:
adding a preservative into the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the step (2) to obtain a finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus;
the preservative is preferably sodium benzoate;
the preservative in the finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is preferably 0.3% by mass;
the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus can be further prepared into powder, oral liquid or granules and is subpackaged into 100-1000 g/dose (bags or bottles);
the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is applied to preparing a product for preventing and treating the poultry group I adenovirus;
the principle of the invention is as follows:
salvia miltiorrhiza has the functions of nourishing blood, removing qi stagnation of the heart and abdomen, and removing pathogenic wind and keeping heat. Herba et Gemma Agrimoniae and herba Euphorbiae Humifusae have effects of clearing away heat and toxic materials, promoting diuresis, eliminating jaundice, promoting blood circulation, stopping bleeding, and astringing to stop dysentery. Poria has effects of inducing resuscitation and removing dampness, inducing resuscitation and inducing resuscitation to promote heart and intelligence, and promoting fluid production; removing dampness can expel water and dry spleen, strengthen middle-jiao and invigorate stomach; to dispel fright epilepsy and to thicken the intestine. Chi Shi Zhi astringes intestine, stops bleeding, promotes tissue regeneration and heals wound. The liquorice tonifies spleen and qi, clears heat and toxic materials, eliminates phlegm and stops coughing, relieves spasm and pain, and harmonizes the medicines. The whole formula has the effects of promoting blood circulation, removing blood stasis, invigorating spleen, inducing diuresis, relieving swelling and stopping dysentery.
For non-diseased poultry: the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is taken by the whole poultry in a mode of drenching, drinking or mixing, the dosage of each poultry is equivalent to 0.1g of the original medicinal material, and the prevention effect is obvious.
For diseased poultry: 0.1-0.2 mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is irrigated to each bird, is equivalent to 0.1-0.2 g of crude drug, is used for 2 times a day, and is continuously used for 3-5 days; or the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is drunk in a drinking water mode (100 kg of water is added in each 100mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus), and is freely drunk for 3-5 days, so that the treatment effect is obvious.
Compared with the prior art, the invention has the following advantages and effects:
(1) the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is simple in preparation method and convenient to use, can prevent and treat the poultry group I adenovirus, and can obviously reduce the loss of farmers caused by the disease.
(2) During the period of high disease incidence of poultry, the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus provided by the invention is directly taken by irrigation, drinking or mixing, so that the disease resistance of the poultry without disease incidence can be improved, and the disease incidence is reduced. For poultry suffering from diseases (infected with group I adenovirus), the traditional Chinese medicine composition can achieve satisfactory effect by being taken by drenching or freely drinking water.
Detailed Description
The present invention will be described in further detail with reference to examples, but the embodiments of the present invention are not limited thereto.
Example 1
A traditional Chinese medicine composition for preventing and treating I-group adenovirus of poultry is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus comprises the following steps:
(1) pulverizing and mixing the raw material components (90 parts of each of radix salviae miltiorrhizae and agrimony, 30 parts of each of humifuse euphorbia herb, tuckahoe, halloysite and liquorice) of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, adding the mixture into an extraction tank, injecting clear water, soaking and rinsing, and removing impurities and dust; adding clear water with the mass being 8 times that of the raw material components which are rinsed completely, and soaking for 8 hours; then, refluxing and decocting for 3 hours by using steam, and filtering to obtain filtrate and filter residue; adding 4 times of clear water by mass into the filter residue, performing steam reflux decoction for 2h, filtering, and combining the filtrates for 2 times;
(2) concentrating the filtrate prepared in the step (1) to a specific gravity of 1.15, and adjusting the pH to 5.0 by using 1% hydrochloric acid by mass fraction to obtain the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus;
(3) and (3) adding sodium benzoate into the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the step (2) for preservation to obtain a finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, wherein the mass fraction of the sodium benzoate in the finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is 0.3%.
For non-diseased broilers: the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is taken by the whole group in a pouring, drinking or mixing mode, the dosage of each chicken is equivalent to 0.1g of the original medicinal material, and the prevention effect is obvious.
For diseased chickens that have already developed: 0.1-0.2 mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is irrigated to each animal, is equivalent to 0.1-0.2 g of crude drug, is used for 2 times a day, and is continuously used for 3 days; or drinking the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus in a drinking water mode (100 kg of water is added in each 100mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus), freely drinking for 3 days, and having remarkable treatment effect.
Example 2
A traditional Chinese medicine composition for preventing and treating I-group adenovirus of poultry is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus comprises the following steps:
(1) pulverizing and mixing the raw material components (130 parts of radix Salviae Miltiorrhizae and herba Agrimoniae, 60 parts of herba Euphorbiae Humifusae, Poria, Halloysitum rubrum and radix Glycyrrhizae) of the Chinese medicinal composition for preventing and treating poultry group I adenovirus, adding into an extraction tank, injecting clear water, soaking and rinsing, and removing impurities and dust; adding clear water with the mass being 10 times that of the rinsed raw material components, and soaking for 10 hours; then, refluxing and decocting for 2 hours by using steam, and filtering to obtain filtrate and filter residue; adding 3 times of clear water into the filter residue, refluxing and decocting for 1.5h with steam, filtering, and mixing the filtrates for 2 times;
(2) concentrating the filtrate prepared in the step (1) to a specific gravity of 1.05, and adjusting the pH to 4.5 by using 1% hydrochloric acid by mass fraction to obtain the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus;
(3) and (3) adding sodium benzoate into the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the step (2) for preservation to obtain a finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, wherein the mass fraction of the sodium benzoate in the finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is 0.3%.
For non-diseased broilers: the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is taken by the whole group in a pouring, drinking or mixing mode, the dosage of each chicken is equivalent to 0.1g of the original medicinal material, and the prevention effect is obvious.
For diseased chickens that have already developed: 0.1-0.2 mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is irrigated to each animal, is equivalent to 0.1-0.2 g of crude drug, is used for 2 times a day, and is continuously used for 3 days; or drinking the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus in a drinking water mode (100 kg of water is added in each 100mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus), freely drinking for 3 days, and having remarkable treatment effect.
Example 3
A traditional Chinese medicine composition for preventing and treating I-group adenovirus of poultry is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus comprises the following steps:
(1) pulverizing and mixing the raw material components (20 parts of each of radix Salviae Miltiorrhizae and herba Agrimoniae, and 10 parts of each of herba Euphorbiae Humifusae, Poria, Halloysitum rubrum and radix Glycyrrhizae) of the Chinese medicinal composition for preventing and treating poultry group I adenovirus, adding into an extraction tank, injecting clear water, soaking and rinsing, and removing impurities and dust; adding clear water with the mass being 9 times that of the raw material components which are rinsed clean, and soaking for 9 hours; then, refluxing and decocting for 2.5h by using steam, and filtering to obtain filtrate and filter residue; adding clear water with the mass of 3.5 times into filter residue, performing steam reflux and decoction for 1.8h, filtering, and combining the filtrates for 2 times;
(2) concentrating the filtrate prepared in the step (1) to a specific gravity of 1.10, and adjusting the pH to 5.5 by using 1% hydrochloric acid by mass fraction to obtain the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus;
(3) and (3) adding sodium benzoate into the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the step (2) for preservation to obtain a finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, wherein the mass fraction of the sodium benzoate in the finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is 0.3%.
For non-diseased broilers: the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is taken by the whole group in a pouring, drinking or mixing mode, the dosage of each chicken is equivalent to 0.1g of the original medicinal material, and the prevention effect is obvious.
For diseased chickens that have already developed: 0.1-0.2 mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is irrigated to each animal, is equivalent to 0.1-0.2 g of crude drug, is used for 2 times a day, and is continuously used for 3 days; or drinking the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus in a drinking water mode (100 kg of water is added in each 100mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus), freely drinking for 3 days, and having remarkable treatment effect.
Example 4
A traditional Chinese medicine composition for preventing and treating I-group adenovirus of poultry is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus comprises the following steps:
(1) pulverizing and mixing the raw material components (130 parts of radix Salviae Miltiorrhizae and herba Agrimoniae respectively, 20 parts of herba Euphorbiae Humifusae, Poria, Halloysitum rubrum and radix Glycyrrhizae respectively) of the Chinese medicinal composition for preventing and treating poultry group I adenovirus, adding into a cooking pot, injecting clear water, soaking and rinsing, and removing impurities and dust; adding clear water with the mass 6 times of that of the rinsed raw material components, and soaking for 10 hours; decocting for 2h, and filtering to obtain filtrate 1 and residue 1; adding 2 times of clear water by mass into the filter residue 1, decocting for 2h, and filtering to obtain a filtrate 2 and a filter residue 2; adding 2 times of clear water into the filter residue 2, and decocting for 2 h; filtering to obtain filtrate 3 and residue 3, and mixing the filtrates for 3 times;
(2) concentrating the filtrate prepared in the step (1) to a specific gravity of 1.05, and adjusting the pH to 4.5 by using 1% hydrochloric acid by mass fraction to obtain the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus;
(3) and (3) adding sodium benzoate into the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the step (2) for preservation to obtain a finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, wherein the mass fraction of the sodium benzoate in the finished traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is 0.3%.
For non-diseased broilers: the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is taken by the whole group in a pouring, drinking or mixing mode, the dosage of each chicken is equivalent to 0.1g of the original medicinal material, and the prevention effect is obvious.
For diseased chickens that have already developed: 0.1-0.2 mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus is irrigated to each animal, is equivalent to 0.1-0.2 g of crude drug, is used for 2 times a day, and is continuously used for 3 days; or drinking the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus in a drinking water mode (100 kg of water is added in each 100mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus), freely drinking for 3 days, and having remarkable treatment effect.
COMPARATIVE EXAMPLE 1 (No Salvia miltiorrhiza)
A traditional Chinese medicine composition is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition is the same as that of example 4.
COMPARATIVE EXAMPLE 2 (without Agrimonia pilosa and humifuse euphorbia)
A traditional Chinese medicine composition is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition is the same as that of example 4.
COMPARATIVE EXAMPLE 3 (without Poria)
A traditional Chinese medicine composition is prepared from the following raw material components in parts by mass:
the preparation method of the traditional Chinese medicine composition is the same as that of example 4.
Effect example 1
(1) Shandong sunshine farmer Wangzhi:
200 broilers at 3 weeks old are randomly selected from the test chicken farm as a treatment group, and 200 broilers at 3 weeks old are selected as blank controls. 10 chickens with the disease onset in the market and definite diagnosis as group I adenoviruses, which are shown as growth retardation, liver injury and glandular stomach erosion, are taken and put into each test chicken group (a treatment group and a blank control group) for mixed culture and infection test. The treatment group is prevented and treated for 5 days on the day of adding the composition into the infection group, and the specific method comprises the following steps: 100kg of water is added into 100mL of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the embodiment 1, and the treated group chickens can drink freely for 5 days; the blank control group was only observed, and after one week, the change of chicken flocks and the productivity were observed.
(2) Liu of Jining breeding households: the experimental method is the same as the step (1), wherein the preventive and therapeutic medicine is the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the example 2;
(3) full federal experimental chicken farm: the experimental method is the same as the step (1), wherein the preventive and therapeutic drug is the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus prepared in the example 3;
after one week of experiment, the change and production performance of chicken flocks are shown in table 1, and as can be seen from table 1, the traditional Chinese medicine composition for preventing and treating the poultry I-flock adenovirus prepared in the examples 1-3 can effectively reduce the morbidity and mortality of adenovirus infection in the breeding process. The composition has good prevention and treatment effect on group I adenovirus.
TABLE 1 preventive and therapeutic effects of the Chinese medicinal composition for preventing and treating group I adenovirus of poultry prepared in examples 1 to 3
Effect example 2
Selecting 1000 broilers infected by the poultry I group adenovirus infected chicken group, determining the infected broilers of 4 weeks old, and randomly dividing the broilers into 5 groups: group 1: the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus, prepared in the embodiment 1, is used for drenching 0.1mL each time and is taken twice a day; group 2: drinking water by liquorice and gall oral liquid (purchased in the market) and treating by matching with the drinking water of the compound vitamin oral liquid, wherein the dosage is carried out according to the instruction; group 3: using poultry adenovirus antibodies (purchased from market) for injection treatment, and injecting 0.5mL of the antibody per injection according to the instruction; group 4: 0.2mL of antipyretic and antitoxic oral liquid (purchased from market) is used for drinking water matched with 3 wt% of glucose and vitamin C; group 5: as a blank control. Mortality and weight comparisons were recorded 4 days after treatment for each group and compared to the time to return to normal weight after continued feeding for 10 days. The results are shown in Table 2.
By comparison of table 2, it can be found that the traditional Chinese medicine composition for preventing and treating the poultry group i adenovirus prepared in example 1 has a high cure rate, a significant treatment effect on the poultry group i adenovirus infection, short treatment time, fast weight recovery time, and a mortality rate lower than that of other schemes and antibody treatments in the prior art, and can significantly reduce the loss of farmers due to the disease.
TABLE 2 therapeutic Effect of the Chinese medicinal composition for prevention and treatment of group I adenovirus of poultry prepared in example 1
| Group of | Number of experimental animals | Average body weight (kg) of experiment | Mortality (%) | Average body weight (kg) after two weeks |
| 1 | 200 | 1.39 | 1.3 | 2.561 |
| 2 | 200 | 1.41 | 5.1 | 2.108 |
| 3 | 200 | 1.37 | 1.24 | 2.540 |
| 4 | 200 | 1.42 | 4.7 | 2.07 |
| 5 | 200 | 1.40 | 14.6 | 1.97 |
Effect example 3
50 AA white feather broilers of 3 weeks old are pre-mixed to determine to be infected with group I adenovirus. Then, 5 groups are randomly divided into 1-4 groups of experiments, and the 6 th group is an infected group, wherein the 1-4 groups are respectively irrigated with 0.2mL of the traditional Chinese medicine composition prepared in the comparative examples 1-3 and the traditional Chinese medicine composition prepared in the example 4, and the traditional Chinese medicine composition is continuously used for 5 days, and 10 healthy chicken flocks (AA white feather broilers) are selected as the 5 th group (healthy control group). The experiments were conducted in the federal laboratory animal house in the world. The groups were observed to show statistical mortality and growth rates. The results are shown in Table 3.
As can be seen from table 3: the treatment and rehabilitation effects of the compositions with different components are very different, wherein the removal of salvia miltiorrhiza results in poor control of death, the removal of agrimony, humifuse euphorbia herb and poria cocos results in obvious reduction of growth speed, and the traditional Chinese medicine composition prepared in the example 4 has the best effect.
TABLE 3 therapeutic Effect of different Chinese medicinal compositions
| Group of | Number of | Initial body weight | Number of deaths after one week | Mean body weight after one week | Mean body weight after two weeks |
| Comparative example 1 | 10 | 867g | 3 | 1101g | 1830g |
| Comparative example 2 | 10 | 885g | 2 | 1087g | 1770g |
| Comparative example 3 | 10 | 872g | 1 | 1113g | 1925g |
| Example 4 | 10 | 877g | 1 | 1120g | 1997g |
| Healthy control group | 10 | 925g | 0 | 1196g | 2035g |
| Infectious group | 10 | 875g | 3 | 1067g | 1719g |
Effect example 4
The SD rat (purchased from the laboratory animal center of Qingdao agricultural university) is quarantined for about 1-2 weeks, and the abnormal clinical rat is abandoned. 180-240 g of rats which have not been mated and pregnant are selected, and the rats are half female and half male. 10 groups of rats are selected in total, five groups of rats are selected for preliminary experiments, and the number of the rats in each group is 5, so that LD 0-LD 100 of the traditional Chinese medicine composition for preventing and treating the poultry I-group adenovirus prepared in the embodiments 1-4 are obtained. Five groups of rats were tested for LD50 of the composition prepared in examples 1-4 for the prevention and treatment of poultry group I adenovirus, wherein each group had 10 rats. Rats were fasted overnight prior to infection. The experimental result shows that the acute oral (LD50) > 500mg/kg is actually low-toxicity.
The traditional Chinese medicine composition for preventing and treating poultry group I adenovirus provided by the invention is simple in preparation method and convenient to use, and can obviously reduce the loss of poultry farmers caused by the disease for preventing and treating poultry group I adenovirus. In particular, the composition is taken by irrigation, drinking water or mixing with feed during the high incidence period of the broiler chickens, so that the disease resistance of the chicken flocks can be improved. The treatment effect is obvious when the sick group I adenovirus chicken flocks are drenched or drunk.
The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.
Claims (10)
1. A traditional Chinese medicine composition for preventing and treating I-group adenovirus of poultry is characterized by being prepared from the following raw material components in parts by mass:
2. the traditional Chinese medicine composition for preventing and treating poultry group I adenovirus according to claim 1 is characterized by being prepared from the following raw material components in parts by mass:
3. the traditional Chinese medicine composition for preventing and treating poultry group I adenovirus according to claim 1 is characterized by being prepared from the following raw material components in parts by mass:
4. the preparation method of the traditional Chinese medicine composition for preventing and treating poultry group I adenovirus as claimed in any one of claims 1 to 3, characterized by comprising the following steps:
(1) mixing the raw material components of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus with water, decocting, filtering, and combining the filtrates;
(2) concentrating the filtrate prepared in the step (1) to obtain a concentrated solution; and adjusting the pH value of the concentrated solution to 4.5-5.5 to obtain the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus.
5. The method for preparing a Chinese medicinal composition for preventing and treating poultry group I adenovirus according to claim 4, which is characterized by comprising the following steps:
the decocting time in the step (1) is 2-4 times, and each time lasts for 1.5-3 hours.
6. The method for preparing a Chinese medicinal composition for preventing and treating poultry group I adenovirus according to claim 4, which is characterized by comprising the following steps:
the decoction in the step (1) is steam reflux decoction;
the specific operation of the steam reflux decoction is as follows: mixing each raw material component of the traditional Chinese medicine composition for preventing and treating the poultry group I adenovirus with water with the mass of 8-10 times, soaking for 8-10 hours, carrying out reflux decoction for 2-3 hours by using steam, and filtering to obtain filtrate and filter residue; mixing the filter residue with 3-4 times of water by mass, and carrying out reflux decoction for 1.5-2 h by using steam.
7. The method for preparing a Chinese medicinal composition for preventing and treating poultry group I adenovirus according to claim 4, which is characterized by comprising the following steps:
the specific gravity of the concentrated solution obtained in the step (2) is 1.05-1.15.
8. A product for preventing and treating I group adenovirus of poultry is characterized by being obtained by the following method:
a poultry group I adenovirus control product is obtained by adding a preservative into the poultry group I adenovirus control traditional Chinese medicine composition prepared in the step (2) in the step (4).
9. The composition of claim 8 for controlling group i adenoviruses in poultry, wherein:
the poultry group I adenovirus prevention and treatment product is prepared into powder, oral liquid or granules and is packaged into 100-1000 g/dose.
10. The use of the Chinese medicinal composition for preventing and treating poultry group I adenovirus according to any one of claims 1 to 3 in the preparation of a product for preventing and treating poultry group I adenovirus.
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