CN108375572A - Detection method and device based on chemoluminescence method - Google Patents
Detection method and device based on chemoluminescence method Download PDFInfo
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- CN108375572A CN108375572A CN201810160277.9A CN201810160277A CN108375572A CN 108375572 A CN108375572 A CN 108375572A CN 201810160277 A CN201810160277 A CN 201810160277A CN 108375572 A CN108375572 A CN 108375572A
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- 238000000034 method Methods 0.000 title claims abstract description 45
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- VDGJOQCBCPGFFD-UHFFFAOYSA-N oxygen(2-) silicon(4+) titanium(4+) Chemical compound [Si+4].[O-2].[O-2].[Ti+4] VDGJOQCBCPGFFD-UHFFFAOYSA-N 0.000 claims abstract description 107
- 239000000427 antigen Substances 0.000 claims abstract description 40
- 102000036639 antigens Human genes 0.000 claims abstract description 40
- 108091007433 antigens Proteins 0.000 claims abstract description 40
- 238000006243 chemical reaction Methods 0.000 claims abstract description 27
- 230000011514 reflex Effects 0.000 claims abstract description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 34
- 239000003550 marker Substances 0.000 claims description 22
- 239000000377 silicon dioxide Substances 0.000 claims description 17
- 238000000926 separation method Methods 0.000 claims description 9
- 230000005284 excitation Effects 0.000 claims description 6
- 230000001427 coherent effect Effects 0.000 claims description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 5
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 description 5
- 229910052710 silicon Inorganic materials 0.000 description 5
- 239000010703 silicon Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 235000010215 titanium dioxide Nutrition 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002796 luminescence method Methods 0.000 description 2
- 239000004408 titanium dioxide Substances 0.000 description 2
- 229910003978 SiClx Inorganic materials 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003760 hair shine Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- SOQBVABWOPYFQZ-UHFFFAOYSA-N oxygen(2-);titanium(4+) Chemical class [O-2].[O-2].[Ti+4] SOQBVABWOPYFQZ-UHFFFAOYSA-N 0.000 description 1
- 230000005622 photoelectricity Effects 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Plasma & Fusion (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of detection method based on chemoluminescence method, this method includes：The multiple titanium dioxide silicon chips for being adsorbed with antibody are placed in the detected sample comprising a variety of antigens；It waits for that the antigen-reactive in the antibody and detected sample entrained by titanium dioxide silicon chip is complete, is handled by the coated titanium dioxide silicon chip of antigen-antibody institute that the reaction was complete according to chemoluminescence method, obtain multiple titanium dioxide silicon chips to be detected；Multiple titanium dioxide silicon chips to be detected are detached, are irradiated by light source so that multiple titanium dioxide silicon chips to be detected after detaching are inspired multigroup fluorescence, and reflex to photomultiplier acquisition process and obtain the multi-group light-emitting amount corresponding to fluorescence；The incidence relation between luminous quantity and antigen corresponding to titanium dioxide silicon wafer thickness, is associated with to obtain a variety of antigens in detected sample by multi-group light-emitting amount.A variety of antigens that a detected sample is included can be detected using the present invention simultaneously, be effectively improved detection efficiency.
Description
Technical field
The invention belongs to technical field of biological more particularly to a kind of detection methods and dress based on chemoluminescence method
It sets.
Background technology
Currently, the detection method based on chemoluminescence method is commonly used in detecting various diseases, such as fruit disease in hospital for patient
The project kind checked required for people is various, generally requires patient and provides more parts of detected samples, such as blood sample, i.e., clinical
The upper a variety of antigens of detection need more parts of detected samples, time-consuming and laborious, and cause detection efficiency low.
Invention content
The embodiment of the present invention is designed to provide a kind of detection method and device based on chemoluminescence method, it is intended to solve
A detected sample is only used for detecting a kind of antigen in the prior art, and the problem for causing detection efficiency low.
Technical solution used by the embodiment of the present invention is as follows：
On the one hand, a kind of detection method based on chemoluminescence method, including：Multiple titanium dioxide silicon chips of antibody will be adsorbed with
It is placed in the detected sample comprising a variety of antigens, the thickness difference of each titanium dioxide silicon chip and the antibody difference adsorbed；
Wait for that the antigen-reactive in the antibody and detected sample entrained by the titanium dioxide silicon chip is complete, according to chemoluminescence method handle by
The coated titanium dioxide silicon chip of Ag-Ab institute that the reaction was complete, obtains multiple titanium dioxide silicon chips to be detected；Detach the multiple wait for
Detected silica piece, the multiple titanium dioxide silicon chips to be detected irradiated by light source after to detach are inspired multigroup fluorescence,
And reflex to photomultiplier acquisition process and obtain the multi-group light-emitting amount corresponding to fluorescence, each group of luminous quantity corresponds to one kind two
Oxidized silicon chip thickness；The incidence relation between luminous quantity and antigen corresponding to titanium dioxide silicon wafer thickness, by multi-group light-emitting
Amount association obtains a variety of antigens in the detected sample.
Preferably, the thickness of the titanium dioxide silicon chip is between 40 microns to 60 microns.
Preferably, the light source is laser, and centre wavelength is 840 nanometers.
Preferably, the wavelength of multigroup fluorescence is between 400 nanometers to 800 nanometers.
Preferably, the multiple titanium dioxide silicon chip to be detected of separation, including：By the multiple silica to be detected
Piece is layed in a plane.
Preferably, the multiple titanium dioxide silicon chips to be detected irradiated by light source after to detach are inspired multigroup glimmering
Light, including：Coherent light is sent out by the light source and exposes to diaphotoscope；It is transmitted through via the diaphotoscope multiple to be checked after separation
Survey titanium dioxide silicon chip；Multiple titanium dioxide silicon chips to be detected after separation are inspired multigroup fluorescence, and reflex to the photoelectricity times
Increase pipe.
It is preferably, described to wait for that the antigen-reactive in antibody and detected sample entrained by the titanium dioxide silicon chip is complete,
It is handled by the coated titanium dioxide silicon chip of Ag-Ab institute that the reaction was complete according to chemoluminescence method, obtains multiple dioxies to be detected
SiClx piece, including：Wait for that the antigen-reactive in the antibody and detected sample entrained by the titanium dioxide silicon chip is complete, for reaction
The coated titanium dioxide silicon chip of complete Ag-Ab institute, carries out the detected sample comprising Excess antibody and cleans；To the reaction was complete
The coated titanium dioxide silicon chip of Ag-Ab institute be passed through fluorescent marker so that the fluorescent marker in the silica
Antigen reacts completely, and then is bound to the silica on piece；For the titanium dioxide silicon chip for combining fluorescent marker, to excess
Fluorescent marker cleaned, form the multiple titanium dioxide silicon chip to be detected.
On the other hand, a kind of detection device based on chemoluminescence method, including：Placement module, for antibody will to be adsorbed with
Multiple titanium dioxide silicon chips be placed in the detected sample comprising a variety of antigens, the thickness of each titanium dioxide silicon chip is different and institute
The antibody of absorption is different；Reaction module, for waiting for the antigen in the antibody and detected sample entrained by the titanium dioxide silicon chip
The reaction was complete, handles by the coated titanium dioxide silicon chip of Ag-Ab that the reaction was complete, obtains multiple to be checked according to chemoluminescence method
Survey titanium dioxide silicon chip；Excitation module, for detaching the multiple titanium dioxide silicon chip to be detected, after being irradiated by light source so that detaching
Multiple titanium dioxide silicon chips to be detected be inspired multigroup fluorescence, and reflex to photomultiplier acquisition process obtain correspond to it is glimmering
The multi-group light-emitting amount of light, each group of luminous quantity correspond to a titanium dioxide silicon chip；Detection module, for according to titanium dioxide silicon chip
Incidence relation between luminous quantity and antigen corresponding to thickness is associated with to obtain more in the detected sample by multi-group light-emitting amount
Kind antigen.
Preferably, the thickness of the titanium dioxide silicon chip is between 40 microns to 60 microns.
On the other hand, a kind of device based on chemoluminescence method, including processor and memory store on the memory
There is computer-readable instruction, when the computer-readable instruction is executed by the processor, realizes as described above based on chemistry
The detection method of luminescence method.
The technical solution provided by the embodiments of the present invention is it is found that by multiple titanium dioxide silicon chips of antibody are adsorbed with extremely
In the detected sample comprising a variety of antigens, the thickness difference of each titanium dioxide silicon chip and the antibody difference adsorbed, with
This obtains the luminous quantity corresponding to each titanium dioxide silicon wafer thickness, and then the luminous quantity corresponding to titanium dioxide silicon wafer thickness
Incidence relation between antigen just can be associated with to obtain a variety of antigens in detected sample by multi-group light-emitting amount, effectively
Improve detection efficiency.
Description of the drawings
Fig. 1 is the flow diagram for the detection method based on chemoluminescence method that one embodiment of the invention provides.
Fig. 2 is the realization schematic diagram for the titanium dioxide silicon chip forming process to be detected that one embodiment of the invention provides.
Fig. 3 is titanium dioxide silicon chip moderate resistance body combination antigen, the molecule after fluorescent marker that one embodiment of the invention provides
Structural schematic diagram.
Fig. 4 is the realization schematic diagram for the fluorescence excitation process that one embodiment of the invention provides.
Fig. 5 is the structure chart for the detection device based on chemoluminescence method that one embodiment of the invention provides.
Specific implementation mode
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to the accompanying drawings and embodiments, right
The present invention is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, and
It is not used in the restriction present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below
It does not constitute a conflict with each other and can be combined with each other.
Referring to Fig. 1, in one embodiment, a kind of detection method based on chemoluminescence method may comprise steps of：
Step 110, the multiple titanium dioxide silicon chips for being adsorbed with antibody are placed in the detected sample comprising a variety of antigens.
Wherein, the thickness difference of each titanium dioxide silicon chip and the antibody difference adsorbed.Preferably, titanium dioxide silicon chip
Thickness is between 40 microns to 60 microns.
Specifically, detected sample is passed into sample holder ware, then different antibodies will be adsorbed with and thickness is different
Titanium dioxide silicon chip is placed in sample holder ware, to provide foundation for a variety of antigens detections in follow-up progress detected sample.
Step 120, wait for that the antigen-reactive in the antibody and detected sample entrained by titanium dioxide silicon chip is complete, according to chemistry
Luminescence method processing obtains multiple titanium dioxide silicon chips to be detected by the coated titanium dioxide silicon chip of Ag-Ab institute that the reaction was complete.
In an embodiment in the specific implementation, as shown in Fig. 2, titanium dioxide silicon chip forming process to be detected may include following
Step：
Step 210, wait for that the antigen-reactive in the antibody and detected sample entrained by titanium dioxide silicon chip is complete, for reaction
The coated titanium dioxide silicon chip of complete Ag-Ab institute, carries out the detected sample comprising Excess antibody and cleans.
That is, due in detected sample antigen reacted completely, that cleaned is detected sample and two
The Excess antibody that silica carries so that titanium dioxide silicon chip is only coated with by the Ag-Ab that the reaction was complete.
Coating, refers to that titanium dioxide silicon chip is covered by the Ag-Ab that the reaction was complete.
Step 220, it is passed through fluorescent marker to the coated titanium dioxide silicon chip of Ag-Ab institute that the reaction was complete so that glimmering
Signal object reacts completely with antigen in the silica, and then is bound to the silica on piece.
Fluorescent marker, it is understood that be luminous agent, can shine under external conditioning, formed for acquisition with this
Optical signal.
External condition can be reaction reagent, such as hydrogen peroxide so that reaction reagent is reacted with fluorescent marker, in turn
So that fluorescent marker shines；Alternatively, can also be light source, such as the laser lamp that laser emits so that fluorescent marker exists
Light source irradiation is lower to shine, and is defined not to this in the present embodiment.
It is directed to the coated silica of Ag-Ab institute that the reaction was complete as a result, fluorescent marker is passed through the titanium dioxide
In silicon so that fluorescent marker reacts completely with titanium dioxide silicon chip moderate resistance original, in order to which follow-up silica can be because of its institute
In conjunction with fluorescent marker and be inspired multigroup fluorescence.
Step 230, for the titanium dioxide silicon chip for combining fluorescent marker, excessive fluorescent marker is cleaned,
Form multiple titanium dioxide silicon chips to be detected.
In the present embodiment, cleaning is to be directed to extra fluorescent marker so that titanium dioxide silicon chip is only anti-by what the reaction was complete
Antigen-antibody-fluorescent marker is coated with.
As shown in figure 3, titanium dioxide silicon chip to be detected, be in silica antibody respectively with antigen in detected sample,
Fluorescent marker is formed by after the reaction was complete.
Step 130, multiple titanium dioxide silicon chips to be detected are detached, multiple to be detected two made after detaching are irradiated by light source
Oxidized silicon chip is inspired multigroup fluorescence, and reflexes to photomultiplier acquisition process and obtain the multi-group light-emitting corresponding to fluorescence
Amount, each group of luminous quantity correspond to a kind of titanium dioxide silicon wafer thickness.
In an embodiment in the specific implementation, separation process may comprise steps of：By multiple silica to be detected
Piece is layed in a plane.
Further, based on the multiple titanium dioxide silicon chips to be detected for being layed in a plane, as shown in figure 4, fluorescence excitation mistake
Journey may comprise steps of：
Step 310, coherent light is sent out by light source and exposes to diaphotoscope.
Preferably, light source is laser, and centre wavelength is 840 nanometers.
Step 320, multiple titanium dioxide silicon chips to be detected after separation are transmitted through via diaphotoscope.
Step 330, multiple titanium dioxide silicon chips to be detected after separation are inspired multigroup fluorescence, and reflex to photomultiplier transit
Pipe.
Preferably, the wavelength of multigroup fluorescence is between 400 nanometers to 800 nanometers.
Step 140, the incidence relation between the luminous quantity and antigen corresponding to titanium dioxide silicon wafer thickness, by multigroup hair
Light quantity is associated with to obtain a variety of antigens in detected sample.
Wherein, the incidence relation between the luminous quantity and antigen corresponding to titanium dioxide silicon wafer thickness, is to build and deposit in advance
Storage, that is to say, that same titanium dioxide silicon wafer thickness, if corresponding different luminous quantities, then it represents that not synantigen, i.e. titanium dioxide
Luminous quantity corresponding to silicon wafer thickness realizes the accurate description to antigen in the form of digital quantization.
In other words, different silicon dioxide thickness, even if will be indicative different antigen if corresponding identical luminous quantity.
As a result, obtain corresponding to multiple titanium dioxide silicon chips luminous quantity after, just can according to above-mentioned incidence relation by
Luminous quantity is associated with to obtain the antigen in detected sample.
By process as described above, the detection to a variety of antigens in detected sample is realized, is effectively improved inspection
Survey efficiency.
Referring to Fig. 5, in one embodiment, a kind of detection device 400 based on chemoluminescence method includes but not limited to：It puts
Set module 410, reaction module 420, excitation module 430 and detection module 440.
Wherein, placement module 410 is waited for for being placed in the multiple titanium dioxide silicon chips for being adsorbed with antibody comprising a variety of antigens
It detects in sample, the thickness difference of each titanium dioxide silicon chip and the antibody difference adsorbed.
Reaction module 420 is used to wait for that the antigen-reactive in the antibody and detected sample entrained by titanium dioxide silicon chip to be complete,
It is handled by the coated titanium dioxide silicon chip of Ag-Ab that the reaction was complete according to chemoluminescence method, obtains multiple titanium dioxides to be detected
Silicon chip.
Excitation module 430 is irradiated by light source for detaching multiple titanium dioxide silicon chips to be detected so that multiple after detaching
Titanium dioxide silicon chip to be detected is inspired multigroup fluorescence, and reflexes to photomultiplier acquisition process and obtain corresponding to the more of fluorescence
Group luminous quantity, each group of luminous quantity correspond to a titanium dioxide silicon chip.
Detection module 440 is used for the incidence relation between luminous quantity and antigen corresponding to the thickness according to titanium dioxide silicon chip,
It is associated with to obtain a variety of antigens in detected sample by multi-group light-emitting amount.
In one embodiment, a kind of device based on chemoluminescence method, including processor and memory store on memory
There is computer-readable instruction, when computer-readable instruction is executed by processor, realizes as sent out based on chemistry in above-described embodiment
The detection method of light method.
The above is merely preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
All any modification, equivalent and improvement made by within principle etc., should all be included in the protection scope of the present invention.
Claims (10)
1. a kind of detection method based on chemoluminescence method, which is characterized in that including：
The multiple titanium dioxide silicon chips for being adsorbed with antibody are placed in the detected sample comprising a variety of antigens, each silica
The thickness difference of piece and the antibody difference adsorbed；
Wait for that the antigen-reactive in the antibody and detected sample entrained by the titanium dioxide silicon chip is complete, at chemoluminescence method
The coated titanium dioxide silicon chip of the reason Ag-Ab that the reaction was complete institute, obtains multiple titanium dioxide silicon chips to be detected；
The multiple titanium dioxide silicon chip to be detected is detached, multiple titanium dioxide silicon chips to be detected so that after detaching are irradiated by light source
It is inspired multigroup fluorescence, and reflexes to photomultiplier acquisition process and obtains the multi-group light-emitting amount corresponding to fluorescence, each group
Luminous quantity corresponds to a kind of titanium dioxide silicon wafer thickness；
The incidence relation between luminous quantity and antigen corresponding to titanium dioxide silicon wafer thickness, is associated with to obtain by multi-group light-emitting amount
A variety of antigens in the detected sample.
2. the method as described in claim 1, which is characterized in that the thickness of the titanium dioxide silicon chip 40 microns to 60 microns it
Between.
3. the method as described in claim 1, which is characterized in that the light source is laser, and centre wavelength is 840 nanometers.
4. the method as described in claim 1, which is characterized in that the wavelength of multigroup fluorescence 400 nanometers to 800 nanometers it
Between.
5. the method as described in claim 1, which is characterized in that the multiple titanium dioxide silicon chip to be detected of separation, including：
The multiple titanium dioxide silicon chip to be detected is layed in a plane.
6. the method as described in claim 1, which is characterized in that described to be irradiated by light source so that multiple to be detected after detaching
Titanium dioxide silicon chip is inspired multigroup fluorescence, including：
Coherent light is sent out by the light source and exposes to diaphotoscope；
It is transmitted through multiple titanium dioxide silicon chips to be detected after separation via the diaphotoscope；
Multiple titanium dioxide silicon chips to be detected after separation are inspired multigroup fluorescence, and reflex to the photomultiplier.
7. such as claim 1 to 6 any one of them method, which is characterized in that described to wait for entrained by the titanium dioxide silicon chip
Antibody and the antigen-reactive in detected sample are complete, handle according to chemoluminescence method and are wrapped by the Ag-Ab that the reaction was complete
The titanium dioxide silicon chip of quilt obtains multiple titanium dioxide silicon chips to be detected, including：
Wait for that the antigen-reactive in the antibody and detected sample entrained by the titanium dioxide silicon chip is complete, it is anti-for what the reaction was complete
The coated titanium dioxide silicon chip of antigen-antibody institute carries out the detected sample comprising Excess antibody and cleans；
It is passed through fluorescent marker to the coated titanium dioxide silicon chip of Ag-Ab institute that the reaction was complete so that the fluorescent marker
It is reacted completely with antigen in the silica, and then is bound to the silica on piece；
For the titanium dioxide silicon chip for combining fluorescent marker, excessive fluorescent marker is cleaned, is formed the multiple
Titanium dioxide silicon chip to be detected.
8. a kind of detection device based on chemoluminescence method, which is characterized in that including：
Placement module, for being placed in the multiple titanium dioxide silicon chips for being adsorbed with antibody in the detected sample comprising a variety of antigens,
The thickness difference of each titanium dioxide silicon chip and the antibody difference adsorbed；
Reaction module is pressed for waiting for that the antigen-reactive in the antibody and detected sample entrained by the titanium dioxide silicon chip is complete
According to chemoluminescence method processing by the coated titanium dioxide silicon chip of Ag-Ab that the reaction was complete, multiple silica to be detected are obtained
Piece；
Excitation module is irradiated by light source for detaching the multiple titanium dioxide silicon chip to be detected so that multiple being waited for after detaching
Detected silica piece is inspired multigroup fluorescence, and reflexes to photomultiplier acquisition process and obtain corresponding to the multigroup of fluorescence
Luminous quantity, each group of luminous quantity correspond to a titanium dioxide silicon chip；
Detection module, for the incidence relation corresponding to the thickness according to titanium dioxide silicon chip between luminous quantity and antigen, by multigroup
Luminous quantity is associated with to obtain a variety of antigens in the detected sample.
9. device as claimed in claim 8, which is characterized in that the thickness of the titanium dioxide silicon chip 40 microns to 60 microns it
Between.
10. a kind of device based on chemoluminescence method, which is characterized in that including：
Processor；
Memory is stored with computer-readable instruction on the memory, and the computer-readable instruction is held by the processor
When row, the detection method based on chemoluminescence method as described in any one of claim 1 to 7 is realized.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810160277.9A CN108375572A (en) | 2018-02-26 | 2018-02-26 | Detection method and device based on chemoluminescence method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810160277.9A CN108375572A (en) | 2018-02-26 | 2018-02-26 | Detection method and device based on chemoluminescence method |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997035201A1 (en) * | 1996-03-21 | 1997-09-25 | Ofer Markman | Multi-antigen serological diagnosis |
| CN1320820A (en) * | 2000-04-27 | 2001-11-07 | 赵翀 | Immunodetection method with rich information and its special detection board |
| CN101405603A (en) * | 2006-03-23 | 2009-04-08 | 阿布桑伯Ab公司 | Blood group antigens of different types for diagnostic and therapeutic applications |
-
2018
- 2018-02-26 CN CN201810160277.9A patent/CN108375572A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997035201A1 (en) * | 1996-03-21 | 1997-09-25 | Ofer Markman | Multi-antigen serological diagnosis |
| CN1320820A (en) * | 2000-04-27 | 2001-11-07 | 赵翀 | Immunodetection method with rich information and its special detection board |
| CN101405603A (en) * | 2006-03-23 | 2009-04-08 | 阿布桑伯Ab公司 | Blood group antigens of different types for diagnostic and therapeutic applications |
Non-Patent Citations (1)
| Title |
|---|
| 王小龙等: "《兽医临床病理学》", 中国农业出版社, pages: 169 - 170 * |
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Application publication date: 20180807 |