CN206038700U - Full -automatic protein chip analysis appearance - Google Patents
Full -automatic protein chip analysis appearance Download PDFInfo
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- CN206038700U CN206038700U CN201621021489.1U CN201621021489U CN206038700U CN 206038700 U CN206038700 U CN 206038700U CN 201621021489 U CN201621021489 U CN 201621021489U CN 206038700 U CN206038700 U CN 206038700U
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Abstract
The utility model provides a full -automatic protein chip analysis appearance belongs to research equipment field, which comprises a frame, sample container, observation system, the reaction system, electrical system and moving system, observation system, the reaction system, moving system and electrical system all are connected with the frame, sample container is used for holding the sample, the sample is used for with the reactor contact and takes place the reaction, the frame includes the workstation, the reaction system is including the reaction platform, the reaction platform is connected with the workstation, moving system and reaction system all are connected with the electrical system electricity, the reaction system includes the reactor, observation system is used for observing the reaction condition of reactor, moving system is including the arm that is used for the experiment operation, the arm is used for transferring to the reaction platform with the reactor, the arm still is used for snatching the disposable tip and adds the sample to reactor. This device truns into the step of protein analysis to automation, has reduced cross infection's probability in the experiment, has improved the efficiency and the degree of accuracy of experiment.
Description
Technical field
This utility model is related to study clothing arts, in particular to full-automatic ProteinChip Analysis instrument.
Background technology
At present, the detection technique in medical institutions mainly uses euzymelinked immunosorbent assay (ELISA), Full-automatic chemiluminescence immunity point
The traditional immunization determination techniques of analysis method carry out single index detection.Enzyme linked immunological law technology mainly educates device, enzyme mark by sample-adding plate, ovum
Instrument, board-washing machine, data processing module composition.The ultimate principle of the technology is：1. antigen or antibodies are made to carry to certain solid phase
Body surface face, and keep its immunocompetence.2. antigen or antibody is made to connect into enzyme-labelled antigen or antibody with certain enzyme, this enzyme mark resists
Former or antibody had both retained its immunocompetence, retained the activity of enzyme again.When determining, by inspection specimen (measure antibody therein or
Antigen) and enzyme-labelled antigen or antibody antigen or antibody by different steps with surface of solid phase carriers react.With the side of washing
Method makes the antigen antibody complex formed on solid phase carrier separate with other materials, finally with reference to the enzyme amount on solid phase carrier with
In specimen, the amount of tested substance is into certain ratio.After adding the substrate of enzyme reaction, substrate is changed into color products by enzyme catalysiss, produces
The amount of thing is directly related with the amount of tested substance in specimen, therefore can qualitatively or quantitatively be divided according to the depth of color reaction
Analysis.Chemiluminescent ultimate principle is molecule (reactant, product, intermediate or the fluorescence of certain material in reaction system
Matter) absorb the discharged energy of reaction and by ground state transition to excited state, then ground state is returned from excited state again, while by energy
Amount is discharged in the form of light radiation, produces chemiluminescence, typically by sample disk, reagent disc, incubation reaction disk, electrochemistry inspection
Examining system, computer control system composition.
Utility model people found under study for action, and requirement of the existing technology due to labelling to reagent is complicated, and operating procedure is numerous
Trivial, difficult quantitation, its labelling easily cause cross-contamination, and multinomial marker detection then needs prolonged loaded down with trivial details work, and compared with
High expense, therefore be difficult to promote.
Utility model content
This utility model provides one of a kind of full-automatic ProteinChip Analysis instrument, its purpose, reduces protein
The artificial operation of analysis experiment；The two of its purpose are, it is to avoid the cross-contamination produced in experimentation；The three of its purpose exist
In, the time of shortening protein analyses experiment, the efficiency of raising experiment.
What this utility model was realized in：
A kind of full-automatic ProteinChip Analysis instrument, including frame, shuttle, viewing system, response system, automatically controlled system
System and motor system, the viewing system, response system, motor system and electric-control system are connected with the frame, the sample
Product container is used for accommodating sample, and for contacting and reacting with the reactor, the frame includes workbench to the sample,
The response system includes reaction bench, and the reaction bench is connected with the workbench, the motor system and the response system
Electrically connect with the electric-control system, the response system includes reactor, the viewing system is used for observing the reactor
Response situation, the motor system includes the mechanical arm for carrying out experimental implementation, and the mechanical arm is for by the reaction
Device is transferred to the reaction bench, and the frame also includes suction nozzle box, and the suction nozzle box are used for placing disposable tip, the machinery
Arm is additionally operable to capture the disposable tip and adds the sample into the reactor.
In preferred embodiment of the present utility model, the frame includes guide rail, the mechanical arm and the slide
Connection, the electric-control system are used for controlling the mechanical arm along the slide.
In preferred embodiment of the present utility model, the mechanical arm includes first and second, described first with
The slide connection, described first includes X direction guiding rails, and the X direction guiding rails are arranged along the short transverse of the frame, institute
State second to be slidably connected with the X direction guiding rails, the electric-control system is additionally operable to drive described second to slide along the X direction guiding rails
It is dynamic.
In preferred embodiment of the present utility model, the analyser also includes rotating disk and suction nozzle box, the suction nozzle box
It is connected with the workbench or the frame, the suction nozzle box storage has multiple disposable tips, the rotating disk and the work
Make platform and rotate connection, at the top of the rotating disk, be placed with shuttle described at least one and at least one reactor, it is described
Electric-control system is additionally operable to drive the rotating disk to rotate, and the mechanical arm is additionally operable to capture the disposable tip described to extract
Sample, and the sample is transferred to into the reactor, rotary centerline place direction and the frame of the rotating disk
Short transverse is parallel.
In preferred embodiment of the present utility model, the workbench is additionally provided with for concentrating the disposable tip used up
Discarding mouth, the frame also includes discarded collecting box, and the discarded collecting box is located at the bottom of the workbench, it is described once
Property suction nozzle can from it is described discarding mouth fall into the discarded collecting box.
In preferred embodiment of the present utility model, the frame also includes benchmark table top and matter storage lattice, the matter storage lattice
The bottom of the benchmark table top is arranged on, the workbench is arranged on the top of the benchmark table top.
In preferred embodiment of the present utility model, the reactor includes the chip being detachably connected and reactor master
Body, the surface of the chip are provided with active organism probe, and the active organism probe is set to multiple arrays, and each is biological
Active probe corresponds to a protein array micro unit, and the active organism probe is used for combining target molecule and generates biology
Molecular complex, the viewing system are used for observing the change procedure of active organism probe.
In preferred embodiment of the present utility model, the analyser also includes filling for the upset for overturning the chip
Put, the turning device is connected with the reaction bench, the turning device includes rotatable movable part, the movable part turns
Dynamic centrage level, the chip can rotate 180 ° by the turning device.
In preferred embodiment of the present utility model, the viewing system includes that observation platform, oval thickness component and CCD take the photograph
Camera, the observation platform are connected with the frame, the reactor that the observation platform is used for after placing response, the oval thickness group
Part is used for extending polarized lightwave and irradiates the reactor, and the ccd video camera is due to detecting the sample being loaded with reflecting light
Information is simultaneously stored in the form of generating digital picture.
In preferred embodiment of the present utility model, the analyser also includes the surface residue for purge reactor
Cleaning systems, the cleaning systems are electrically connected with the electric-control system, and the cleaning systems include rinsing table, nitrogen machine, lead
Pipe, syringe pump and soda liquor container, one end of the conduit are connected with the soda liquor container, and the other end of the conduit is pointed to
The rinsing table, the syringe pump is for moving the cleanout fluid in the soda liquor container along conduit and rinse the cleaning
Platform, the rinsing table are connected with the workbench, and the nitrogen machine is used for drying up the reactor on the observation platform, described
Mechanical arm is additionally operable to be rinsed the roof that reacted reactor moves to the rinsing table, and by the reactor after flushing
It is placed in the observation platform.
The beneficial effects of the utility model are：Experimental implementation, kinetic system are carried out using electric-control system powered motion system 200
Mechanical arm in system replaces manually being tested, and alleviates artificial labor by viewing system studying to reactor
It is dynamic.The control of NC postprocessing can effectively reduce the mistake that artificial operating error may be brought, and improve the accuracy of experimental result, plus
The efficiency of fast experiment.
Description of the drawings
In order to be illustrated more clearly that the technical scheme of this utility model embodiment, below will be to using needed for embodiment
Accompanying drawing be briefly described, it will be appreciated that the following drawings illustrate only some embodiments of the present utility model, therefore should not be by
Regard the restriction to scope as, for those of ordinary skill in the art, on the premise of not paying creative work, may be used also
To obtain other related accompanying drawings according to these accompanying drawings.
Fig. 1 is the overall schematic of a kind of full-automatic ProteinChip Analysis instrument that this utility model embodiment 1 is provided；
Front views of the Fig. 2 for Fig. 1；
Rearviews of the Fig. 3 for Fig. 1；
Top views of the Fig. 4 for Fig. 1；
Fig. 5 is the front view of the reactor that this utility model embodiment 2 is provided；
Upward views of the Fig. 6 for Fig. 5；
Sectional views of the Fig. 7 for Fig. 5；
Partial schematic diagrams of the Fig. 8 for Fig. 7；
Fig. 9 is the structural representation of the reactor that this utility model embodiment 3 is provided；
Left views of the Figure 10 for Fig. 9；
Figure 11 is the structural representation of the reactor that this utility model embodiment 4 is provided；
Figure 12 is the structural representation of the reactor that this utility model embodiment 5 is provided.
Reference collects：
Reactor 100；Chip carrier 101；Chip 102；First reaction film 103；Reactor body 104；Projection 105；Second
Reaction film 106；Crawl hole 107；Drainage lumens 109；Feed pathway 110；Liquid outlet channel 111, the first feeding-passage 112；Second is anti-
Answer passage 113；Reaction bench 114；Peristaltic pump 115；Shuttle 200；Sample 210；Viewing system 300；Observation platform 310；It is ellipse inclined
Optical module 320；Ccd video camera 330；Frame 400；Guide rail 410；Benchmark table top 420；Matter storage lattice 430；Workbench 440；Abandon
Mouth 441；Discarded collecting box 450；Electric-control system 500；Motor system 600；Mechanical arm 610；First 611；X direction guiding rails 612；The
Two 613；Rotating disk 700；Suction nozzle box 800；Disposable tip 810；Turning device 900；Movable part 901；Cleaning systems 1000；
Clean hatch 1010.
Specific embodiment
Requirement of the existing technology due to labelling to reagent is complicated, and complex operation step, difficult quantitation, its labelling are easily made
Into cross-contamination, multinomial marker detection then needs prolonged loaded down with trivial details work, and higher expense, therefore is difficult to promote.
In consideration of it, this utility model provides a kind of full-automatic ProteinChip Analysis instrument, by each of Automatic Control
Individual system, eliminates manual operation, improves the efficiency of experiment.
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in this utility model can
To be combined with each other.
It is for making purpose, technical scheme and the advantage of this utility model embodiment clearer, new below in conjunction with this practicality
Accompanying drawing in type embodiment, is clearly and completely described to the technical scheme in this utility model embodiment, it is clear that retouched
The embodiment stated is a part of embodiment of this utility model, rather than the embodiment of whole.Generally described in accompanying drawing herein and
The component of this utility model embodiment for illustrating can be arranged and be designed with a variety of configurations.Therefore, below to attached
The detailed description of the embodiment of the present utility model provided in figure is not intended to limit claimed scope of the present utility model,
But it is merely representative of selected embodiment of the present utility model.Based on the embodiment in this utility model, ordinary skill people
The every other embodiment obtained under the premise of creative work is not made by member, belongs to the model of this utility model protection
Enclose.
In description of the present utility model, it is to be understood that term " " center ", " longitudinal direction ", " horizontal ", " length ", " width
Degree ", " thickness ", " on ", D score, "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outward ", " suitable
The orientation of the instruction such as hour hands ", " counterclockwise " or position relationship are or the reality based on orientation shown in the drawings or position relationship
With novel product using when the orientation usually put or position relationship, or the orientation that usually understands of those skilled in the art or
Position relationship, is for only for ease of description this utility model, rather than indicates or imply that the equipment or element of indication must have
Specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to restriction of the present utility model.
Additionally, term " first ", " second " are only used for describing purpose, and it is not intended that indicating or implying relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can express or
Implicitly include one or more this feature.In description of the present utility model, " multiple " are meant that two or two
More than, unless otherwise expressly limited specifically.
In this utility model, unless otherwise clearly defined and limited, term " installation ", " being connected ", " connection ", " Gu
It is fixed " etc. term should be interpreted broadly, it is for example, it may be fixedly connected, or be detachably connected or integral；Can be
It is mechanically connected, or electrically connects；Can be joined directly together, it is also possible to be indirectly connected to by intermediary, can be two
The connection of element internal or the interaction relationship of two elements.For the ordinary skill in the art, can basis
Concrete condition understands concrete meaning of the above-mentioned term in this utility model.
In this utility model, unless otherwise clearly defined and limited, fisrt feature second feature it " on " or it
D score can include the first and second feature directly contacts, it is also possible to not be directly contact including the first and second features but logical
The other characterisation contact crossed between them.And, fisrt feature second feature " on ", " top " and " above " include the
One feature is directly over second feature and oblique upper, or is merely representative of fisrt feature level height higher than second feature.First is special
Levy second feature " under ", " lower section " and " below " include fisrt feature immediately below second feature and obliquely downward, or only
Represent that fisrt feature level height is less than second feature.
Embodiment 1
Fig. 1-Fig. 4 is referred to, this utility model embodiment 1 provides a kind of 102 analyser of full-automatic protein chip, bag
Including rack 400, shuttle 200, rotating disk 700, turning device 900, cleaning systems 1000, viewing system 300, reaction system
System, electric-control system 500 and motor system 600.
Viewing system 300, cleaning systems 1000, rotating disk 700, turning device 900, response system, 600 and of motor system
Electric-control system 500 is connected with frame 400.
Frame 400 includes workbench 440, and response system includes reaction bench 114, and reaction bench 114 is connected with workbench 440,
Motor system 600 and response system are electrically connected with electric-control system 500, and response system includes reactor 100, viewing system 300
For the response situation of observing response device 100, motor system 600 includes the mechanical arm 610 for carrying out experimental implementation, mechanical arm
610 for being transferred to reaction bench 114 by reactor 100, and frame 400 also includes suction nozzle box 800, and suction nozzle box 800 are used for placing one
Secondary property suction nozzle 810, shuttle 200 be used for accommodate sample 210, sample 210 for contacting and reacting with reactor 100,
Mechanical arm 610 is additionally operable to capture disposable tip 810 and add sample 210 into reactor 100.
Frame 400 includes guide rail 410, and mechanical arm 610 is slidably connected with guide rail 410, and electric-control system 500 is used for control machinery
Arm 610 slides along guide rail 410.
In order to realize the motion of 610 in the vertical direction of mechanical arm, the mechanical arm 610 in the present embodiment includes first
611 and second 613, first 611 is slidably connected with guide rail 410, and first 611 includes X direction guiding rails 612, X direction guiding rails 612
Short transverse along frame 400 is arranged, and second 613 is slidably connected with X direction guiding rails 612, and electric-control system 500 is additionally operable to driving the
Slide along X direction guiding rails 612 for two 613.
Suction nozzle box 800 are connected with workbench 440 or frame 400, and the storage of suction nozzle box 800 has multiple disposable tips 810, revolves
Rotating disk 700 is rotated with workbench 440 and is connected, and the top of rotating disk 700 is placed with least one shuttle 200 and at least one
Reactor 100, electric-control system 500 are additionally operable to drive rotating disk 700 to rotate, and mechanical arm 610 is additionally operable to capture disposable tip 810
To extract sample 210, and sample 210 is transferred to into reactor 100, the rotary centerline place direction of rotating disk 700 and frame
400 short transverse is parallel.
Using the rotational motion of rotating disk 700, the position of the shuttle 200 in rotating disk 700 can be adjusted.It is logical
The manner is crossed, multiple shuttles 200 can be set in the top of rotating disk 700.As mechanical arm 610 slides along guide rail 410,
Therefore, the mobile route of mechanical arm 610 is fixed.When many bottles of shuttles 200 are arranged in rotating disk 700, can pass through automatically controlled
System 500 drives rotating disk 700 to rotate so that shuttle 200 is moved to the lower section of mechanical arm 610, so that mechanical arm 610 is carried
Take sample 210.
Workbench 440 is additionally provided with the discarding mouth 441 for concentrating the disposable tip 810 used up, and frame 400 also includes
Discarded collecting box 450, discards the bottom that collecting box 450 is located at workbench 440, and disposable tip 810 can fall from mouth 441 is abandoned
Enter discarded collecting box 450.
Frame 400 also includes benchmark table top 420 and matter storage lattice 430, and matter storage lattice 430 is arranged on the bottom of benchmark table top 420,
Workbench 440 is arranged on the top of benchmark table top 420.Matter storage lattice 430 can be used not only for storing the related article of experiment, also profit
With the space of frame 400, the height of benchmark table top 420 and workbench 440 is improved, also space is provided to recovery system.
Reactor 100 includes the chip 102 being detachably connected and reactor body 104, and the surface of chip 102 is provided with life
Thing active probe, active organism probe are set to multiple arrays, and each active organism probe corresponds to a protein array
Micro unit, active organism probe are used for combining target molecule and generate bimolecular complexes, and viewing system 300 is used for observing life
The change procedure of thing active probe.Chip 102 can be separated by mechanical arm 610 with reactor body 104.
When sensitive surface is contacted with the solution containing target molecule, if matching somebody with somebody on the target molecule in solution and probe
There is specific binding in base molecule, will generate bimolecular complexes, and surface of solids biomolecule surface density changes, this
Change can be observed by oval thickness imaging system high-resolution.
Turning device 900 is used for turning-over of chip 102, and turning device 900 is connected with reaction bench 114, and turning device 900 includes
Rotatable movable part 901, the rotary centerline level of movable part 901, chip 102 can be rotated by turning device 900
180°。
900 turning-over of chip 102 of turning device so that the top surface of the multielement protein chip 102 of tabular and bottom surface exchange.Upset
Meaning be, the reason of virtual condition when chip 102 is separated from reactor 100 and chip 102 on observation platform 310
Think that state is different, if the information that viewing system 300 is obtained can not caused inaccurate through upset.Utility model people do not have
Directly change the reaction environment of reactor 100 and the layout of viewing system 300, but become by the turning device 900 of addition
The angle of multielement protein chip 102 is changed, in order to observing response result.
In preferred embodiment of the present utility model, viewing system 300 includes observation platform 310,320 and of oval thickness component
Ccd video camera 330, observation platform 310 are connected with frame 400, the reactor 100 that observation platform 310 is used for after placing response, oval thickness
Learning component 320 is used for extending polarized lightwave and irradiates reactor 100, and ccd video camera 330 is due to detecting what is be loaded with reflecting light
210 information of sample is simultaneously stored in the form of generating digital picture.
Ccd video camera 330 refers to the video camera with CCD chip 102.CCD refers to charge-coupled image sensor.It is ellipse by being imaged
The inclined technology of circle, research worker can realize extra small piece of film analysis, ellipsometry in situ, the ellipsometry analysis under various liquid environments
And can realize being combined with multiple technologies.When being utilized to study the combination of albumen, experimenter is without the need for as fluorescent immune method, enzyme
The biomolecule of linked immunosorbent assay and radioimmunology like that to being detected uses the second antibody reagent of labelling, to biological point to be measured
Sub- activity undisturbed and damage.Additionally, the sensitivity of detection is high, the biological membranous layer change of 0.1nm can be recognized.
In the present embodiment, cleaning systems 1000 are electrically connected with electric-control system 500, cleaning systems 1000 include rinsing table,
Nitrogen machine, conduit, syringe pump and soda liquor container, one end of conduit are connected with soda liquor container, and the other end of conduit is pointed to clearly
Wash platform, syringe pump for moving and rinse rinsing table, rinsing table and workbench 440 by the cleanout fluid in soda liquor container along conduit
Connection, nitrogen machine are used for drying up the reactor 100 on observation platform 310, and mechanical arm 610 is additionally operable to reacted reactor
100 roofs for moving to rinsing table are rinsed, and the reactor 100 after flushing is placed in observation platform 310.
Additionally, it is further, in order to avoid the cross infection in experimentation, shuttle 200, disposable tip 810
Disposable product is with reactor 100.
During this device busy, first drive whether all parts unit meets testing conditions, Ran Hou electricity by electric-control system 500
500 powered motion system 600 of control system works, and reactor 100 is transferred to reaction bench 114 by mechanical arm 610.Then mechanical arm
610 capture disposable tip 810 again and draw quantitative sample 210, sample 210 is assigned in enzyme-linked box, then disposably will be inhaled
810 lose into discarding mouth 441.Again PBST and deionized water are separately added in reactor 100.Response system includes peristaltic pump
115, electric-control system 500 drives response system work, peristaltic pump 115 that sample 210 is delivered to the core of reactor 100 as power source
On piece 102.After chip 102 reacts, 500 first driving machine tool arm 610 of electric-control system is independent from reactor 100 by chip 102
Rinsing table is removed and be transferred to, then drives cleaning systems 1000 to work, rinse out the residue on 102 surface of chip, and by chip
102 dry up.Electric-control system 500 drives mechanical arm 610 to operate, and chip 102 is transferred to turning device 900.Electric-control system 500 drives
180 ° of the motion of dynamic turning device 900 upset so that the top surface of chip 102 and bottom surface exchange.After the completion of exchange, mechanical arm 610 is again
Chip 102 is moved to into observation platform 310, chip 102 is analyzed by viewing system 300 and data processing.Finally, mechanical arm
610 abandon the reactor body 104 of chip 102 and reactor 100 to mouth 441 is abandoned, and complete to test or continues beginning next
Secondary experiment.
As shown in figure 4, in whole experiment process, completing the transfer of chip 102, turning device for the ease of mechanical arm 610
900th, rotating disk 700, discarding mouth 441, reaction bench 114, observation platform 310, suction nozzle box 800 and clean hatch 1010 are all located at mechanical arm
610 along the vertical lower section of the motion path of guide rail 410.
Embodiment 2
Fig. 5-Fig. 8 is referred to, this utility model embodiment 2 provides a kind of reactor, anti-including chip carrier 101, first
Answer film 103, reactor body 104 and the chip 102 for detecting course of reaction.
Chip 102 is fixed on the bottom of chip carrier 101, and the roof of the first reaction film 103 is contacted with the diapire of chip 102,
The roof of reactor body 104 is contacted with the diapire of the first reaction film 103, and the main body of reactor is provided with 110 He of feed pathway
Liquid outlet channel 111, feed pathway 110 and liquid outlet channel 111 extend vertically through reactor body 104, the bottom of feed pathway 110
For contacting with reactant liquor, the first reaction film 103 is provided with drainage lumens 109 in portion, feed pathway 110 and liquid outlet channel 111 with
Drainage lumens 109 are connected.
By the feed pathway 110 and liquid outlet channel 111 that arrange carrying out the liquid circulation of reactant liquor, the power of imbibition can
Solve with by external peristaltic pump.The drainage lumens 109 formed between reaction film and reactor body 104 are led to as feed liquor is connected
The exclusive path in road 110 and liquid outlet channel 111 so that the reactant liquor for flowing through drainage lumens 109 can be connect with the first reaction film 103
Touch so that between the first reaction film 103 and reactant liquor, produce reaction, be easy to observation, additionally, the stable reactant liquor for introducing can make
The reaction obtained in drainage lumens 109 persistently occurs, and is easy to analysis.
In preferred embodiment of the present utility model, reactor also includes the second reaction film 106, the second reaction film 106 with
The diapire laminating of reactor body 104, liquid outlet channel 111 are connected with the second reaction film 106.
Second reaction film 106 is used for carrying out another single step reaction so that this device can carry out many experiments, and second step
Reactant liquor is the reacted liquid through the first step.The mode of multistep reaction causes the in hgher efficiency of this device.
The first reaction film 103 and the second reaction film 106 in the present embodiment can be PDMS films.PDMS material cost
It is low, using simple, with having good adhesiveness between silicon chip, and with good chemical inertness.
Reactor body 104 also includes projection 105, and projection 105 is arranged on the bottom of reactor body 104, feed pathway
110 run through projection 105.
The setting of projection 105 causes the bottom port of feed pathway 110 to there is height with the bottom port of liquid outlet channel 111
Difference on direction, and then cause when reactant liquor is drawn, reactant liquor directly will not be contacted with the second reaction film 106, be easy to rule
The path of model reactant liquor.Additionally, the projection 105 of protrusion also allows for being connected the container that feed pathway 110 is located with reactant liquor.
Further, feed pathway 110 is 24, the number of liquid outlet channel 111, drainage lumens 109 and 110 three of feed pathway
Amount is consistent, and each drainage lumens 109 connects a liquid outlet channel 111 and a feed pathway 110 simultaneously.The quantity of projection 105 is 4
Individual and 2 group of bumps of composition, each group of bumps include two projections 105, between two in same group of bumps projection 105
It is provided with second reaction film 106.
Improved by structure, improve the line density of reactant liquor, and cause will not phase between different reactant liquor paths
Mutually disturb, it is to avoid cross-contamination.In this case, the reaction efficiency of the reactant liquor under the control of this device is greatly improved.
The quantity of projection 105 increases, and the quantity of feed pathway 110 also further can increase.Controlling the second reaction film
Higher utilization rate can also be possessed under 106 quantity.
Additionally, the total quantity of the quantity of group of bumps and feed pathway 110 can also be changed, carried out according to practical situation
Change.The situation of only one of which projection 105 or only one of which feed pathway 110 can also realize the purpose of this utility model.
In the present embodiment, chip 102 is micro-fluidic chip.Further, chip 102 is quartz or glass-chip, draws
The table wall in stream chamber is provided with the decorative layer being bonded by coupling agent by organic polymer or protein.
Micro-fluidic chip has that liquid flowing is controllable, it is few to consume sample and reagent, analyze speed tenfold hundreds of times ground
The features such as raising, it is analyzed while can carrying out up to a hundred samples within even shorter time a few minutes, and can be
Line realizes the pretreatment of sample and analysis overall process.Its target is the function whole laboratory, including sampling, dilution, is added
Reagent, reaction, separation, detection etc. are integrated on microchip, and can be used for multiple times.
Organic polymer mainly has polyvinyl alcohol, polyvinylacetate, hydroxyalkyl vinyl cellulose, Polyethylene Glycol, polyacrylamide
Amine, polysaccharide etc.；Protein such as bovine serum albumin.Decorative layer can lift the accuracy of chip detection reaction.
Additionally, chip 102 can also be plastic chip.In order to preferably cater to the demand of this device, chip can be carried out
Corona treatment, photo-irradiation treatment, hydrophilic process or hydrophobicity are processed.
By this device, the assembling of aglucon and the association reaction of specificity one's share of expenses for a joint undertaking can be carried out, implement sample transport, surface
The series of experiments steps such as formatting, aglucon assembling, surface clean, surface-closed and proteins react.
Reactor in the present embodiment is single use, is independent functional unit, can be entered according to particular detection sample
Row targeted design, realizes detecting while nine indexs of blood examination and tumor-marker, possesses reference cell and recessive control unit
Deng high flux reaction member.
Embodiment 3
Fig. 9 and Figure 10 is referred to, this utility model embodiment 3 provides a kind of reactor, including chip carrier 101, first
Reaction film 103, reactor body 104, crawl hole 107, peristaltic pump and the chip 102 for detecting course of reaction.
Chip 102 is fixed on the bottom of chip carrier 101, and the roof of the first reaction film 103 is contacted with the diapire of chip 102,
The roof of reactor body 104 is contacted with the diapire of the first reaction film 103.The main body of reactor is provided with 110 He of feed pathway
Liquid outlet channel 111, feed pathway 110 and liquid outlet channel 111 extend vertically through reactor body 104, the bottom of feed pathway 110
For contacting with reactant liquor, the first reaction film 103 is provided with drainage lumens 109 in portion, feed pathway 110 and liquid outlet channel 111 with
Drainage lumens 109 are connected.The side wall of the side wall and reactor body 104 of chip carrier 101 is provided with crawl hole 107, chip carrier 101
Hole 107 is captured for being connected with support member.In preferred embodiment of the present utility model, reactor also includes the
Two reaction films 106, the second reaction film 106 are fitted with the diapire of reactor body 104, liquid outlet channel 111 and the second reaction film 106
Connect.
Peristaltic pump is connected with liquid outlet channel 111.Peristaltic pump provides the power of reactant liquor flowing, it is ensured that reactant liquor is according to rule
The path flowing drawn.
Crawl hole 107 is used for fixing the position of this device, and chip carrier 101 and reactor body 104 are provided with crawl hole
107 so that active force can be provided to keep the relative position between chip carrier 101 and reactor body 104 when fixed, kept away
Exempt to loosen between chip carrier 101 and reactor body 104, and then make the path generation of reactant liquor obscure.
Embodiment 4
The present embodiment provides a kind of one-time reaction liquid and draws reactor, and which is inhaled with the one-time reaction liquid in embodiment 2
The difference for taking reactor is that feeding-passage is different with the set-up mode of tapping channel, and remaining content refers to embodiment 2,
This repeats no more.
Specifically, in the present embodiment, feeding-passage includes 113 two parts of the first feeding-passage 112 and the second feeding-passage,
Wherein, the first feeding-passage 112 supplies operator's sample introduction, and the second feeding-passage 113 is vertically arranged and with tapping channel 111 simultaneously
Row is arranged.
As shown in figure 11, in the present embodiment, the second feeding-passage 113 is four passages being parallel to each other, the arrow in Figure 11
Head represents the flow direction of reactant liquor.Reactant liquor is added from the first feeding-passage 112 first, is being arranged at tapping channel thereafter
Under the power of 111 ends drives, the reactant liquor in the first feeding-passage 112 is entered in the second feeding-passage 113, and anti-with first
Answer film 103 to react, be extracted thereafter by tapping channel 111.In the present embodiment, tapping channel 111 is similarly 4, goes out per root
Material passage 111 is corresponding with second feeding-passage 113.
Embodiment 5
The present embodiment provides a kind of one-time reaction liquid and draws reactor, and which is inhaled with the one-time reaction liquid in embodiment 2
The difference for taking reactor is that feeding-passage 110 is different with the set-up mode of tapping channel 111, and remaining content refers to embodiment 2
, will not be described here.
Specifically, in the present embodiment, as shown in figure 12, in figure, the direction of arrow represents reactant liquor flow direction in the reactor,
Wherein, the first reaction film 103 is arranged at 104 upper end of reactor body, and the second reaction film is arranged at 104 lower end of reactor body,
Feeding-passage 110 and tapping channel 111 are back and forth arranged in reactor body 104 back and forth, and the reciprocal fluid passage for arranging
Upper and lower ends are contacted with the first reaction film 103 and the second reaction film 106 respectively, and such reactant liquor is at by tapping channel 111
Power drive under in fluid passage between feeding-passage 110 and tapping channel 111 flowing reciprocal back and forth and with the first reaction
106 haptoreaction of film 103 and the second reaction film, can greatly improve reactant liquor and the first reaction film 103 and the second reaction film 106
Between contact area and the response time, improve reaction effect.
Unless specifically stated otherwise, part, step, numerical expression and the numerical value for otherwise illustrating in these embodiments is simultaneously
Scope of the present utility model is not limited.
The device provided by this utility model embodiment, which realizes that principle and the technique effect and preceding method of generation are implemented
Example is identical, is brief description, and device embodiment part does not refer to part, refers to corresponding contents in preceding method embodiment.
In all examples being illustrated and described herein, any occurrence should be construed as merely exemplary, and not
Be as restriction, therefore, other examples of exemplary embodiment can have different values.
It should be noted that：Similar label and letter represent similar terms in following accompanying drawing, therefore, once a certain Xiang Yi
It is defined in individual accompanying drawing, then in subsequent accompanying drawing which further need not be defined and is explained.
Preferred embodiment of the present utility model is the foregoing is only, this utility model is not limited to, for this
For the technical staff in field, this utility model can have various modifications and variations.It is all it is of the present utility model spirit and principle
Within, any modification, equivalent substitution and improvements made etc. are should be included within protection domain of the present utility model.
Claims (10)
1. a kind of full-automatic ProteinChip Analysis instrument, it is characterised in that including frame, shuttle, viewing system, reaction system
System, electric-control system and motor system, the viewing system, response system, motor system and electric-control system are connected with the frame
Connect, the response system includes reactor, the shuttle is used for accommodating sample, the sample is for connecing with the reactor
Touch and react, the frame includes workbench, the response system includes reaction bench, the reaction bench and the workbench
Connection, the motor system and the response system are electrically connected with the electric-control system, and the viewing system is used for observing institute
The response situation of reactor is stated, the motor system includes the mechanical arm for carrying out experimental implementation, and the mechanical arm is used for will
The reactor is transferred to the reaction bench, and the frame also includes suction nozzle box, and the suction nozzle box are used for placing disposable tip,
The mechanical arm is additionally operable to capture the disposable tip and adds the sample into the reactor.
2. analyser according to claim 1, it is characterised in that the frame includes guide rail, the mechanical arm with it is described
Slide connects, and the electric-control system is used for controlling the mechanical arm along the slide.
3. analyser according to claim 2, it is characterised in that the mechanical arm includes first and second, described
First is connected with the slide, and described first includes X direction guiding rails, and the X direction guiding rails are along the height side of the frame
To setting, described second is slidably connected with the X direction guiding rails, and the electric-control system is additionally operable to drive described second along described
X direction guiding rails slide.
4. analyser according to claim 1, it is characterised in that the analyser also includes rotating disk and suction nozzle box, institute
State suction nozzle box to be connected with the workbench or the frame, the suction nozzle box storage has multiple disposable tips, the rotating disk
Rotate with the workbench and be connected, at the top of the rotating disk, be placed with shuttle described at least one and at least one reaction
Device, the electric-control system are additionally operable to drive the rotating disk to rotate, the mechanical arm be additionally operable to capture the disposable tip with
The sample is extracted, and the sample is transferred to into the reactor, the rotary centerline place direction of the rotating disk and institute
The short transverse for stating frame is parallel.
5. analyser according to claim 4, it is characterised in that the workbench is additionally provided with for concentrating use up
The discarding mouth of secondary property suction nozzle, the frame also include discarded collecting box, and the discarded collecting box is located at the bottom of the workbench,
The disposable tip can fall into the discarded collecting box from the discarding mouth.
6. analyser according to claim 1, it is characterised in that the frame also includes benchmark table top and matter storage lattice, institute
The bottom that matter storage lattice is arranged on the benchmark table top is stated, the workbench is arranged on the top of the benchmark table top.
7. analyser according to claim 1, it is characterised in that the reactor includes the chip being detachably connected and anti-
The surface of device main body, the chip is answered to be provided with active organism probe, the active organism probe is set to multiple arrays, each
Individual active organism probe corresponds to a protein array micro unit, and the active organism probe is used for combining target molecule life
Into bimolecular complexes, the viewing system is used for observing the change procedure of active organism probe.
8. analyser according to claim 7, it is characterised in that the analyser is also included for overturning the chip
Turning device, the turning device are connected with the reaction bench, and the turning device includes rotatable movable part, the activity
The rotary centerline level of part, the chip can rotate 180 ° by the turning device.
9. analyser according to claim 1, it is characterised in that the viewing system includes observation platform, oval thickness group
Part and ccd video camera, the observation platform are connected with the frame, the reactor that the observation platform is used for after placing response, described
Oval thickness component is used for extending polarized lightwave and irradiates the reactor, and the ccd video camera is due to detecting in reflecting light
The sample message that is loaded with simultaneously is stored in the form of generating digital picture.
10. analyser according to claim 9, it is characterised in that the analyser is also included for purge reactor
The cleaning systems of surface residue, the cleaning systems are electrically connected with the electric-control system, the cleaning systems include rinsing table,
Nitrogen machine, conduit, syringe pump and soda liquor container, one end of the conduit are connected with the soda liquor container, the conduit
The other end points to the rinsing table, and the syringe pump for moving and rinse the cleanout fluid in the soda liquor container along conduit
The rinsing table, the rinsing table are connected with the workbench, and the nitrogen machine is anti-on the observation platform for drying up
Answer device, the mechanical arm to be additionally operable to be rinsed the roof that reacted reactor moves to the rinsing table, and will rinse
Reactor afterwards is placed in the observation platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621021489.1U CN206038700U (en) | 2016-08-31 | 2016-08-31 | Full -automatic protein chip analysis appearance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621021489.1U CN206038700U (en) | 2016-08-31 | 2016-08-31 | Full -automatic protein chip analysis appearance |
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| CN206038700U true CN206038700U (en) | 2017-03-22 |
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| CN201621021489.1U Active CN206038700U (en) | 2016-08-31 | 2016-08-31 | Full -automatic protein chip analysis appearance |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226540A (en) * | 2016-08-31 | 2016-12-14 | 贵州金玖生物技术有限公司 | Full-automatic protein chip analyzer |
| CN107217109A (en) * | 2017-08-02 | 2017-09-29 | 上海百傲科技股份有限公司 | The control method of slide spotting system |
| CN107449645A (en) * | 2017-08-02 | 2017-12-08 | 上海百傲科技股份有限公司 | Spotting system |
| CN108344697A (en) * | 2018-05-14 | 2018-07-31 | 苏州大学 | A kind of special loading shelf combination of the external sample of optoacoustic tomoscan molecular imaging system |
| WO2021032082A1 (en) * | 2019-08-19 | 2021-02-25 | 江苏汇先医药技术有限公司 | Microfluidic sample processing device |
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2016
- 2016-08-31 CN CN201621021489.1U patent/CN206038700U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106226540A (en) * | 2016-08-31 | 2016-12-14 | 贵州金玖生物技术有限公司 | Full-automatic protein chip analyzer |
| CN107217109A (en) * | 2017-08-02 | 2017-09-29 | 上海百傲科技股份有限公司 | The control method of slide spotting system |
| CN107449645A (en) * | 2017-08-02 | 2017-12-08 | 上海百傲科技股份有限公司 | Spotting system |
| CN107449645B (en) * | 2017-08-02 | 2019-11-15 | 上海百傲科技股份有限公司 | Spotting system |
| CN107217109B (en) * | 2017-08-02 | 2019-12-31 | 上海百傲科技股份有限公司 | Control method of slide sample application system |
| CN108344697A (en) * | 2018-05-14 | 2018-07-31 | 苏州大学 | A kind of special loading shelf combination of the external sample of optoacoustic tomoscan molecular imaging system |
| CN108344697B (en) * | 2018-05-14 | 2024-02-09 | 苏州大学 | Special sample loading frame combination for in-vitro samples of photoacoustic tomography molecular imaging system |
| WO2021032082A1 (en) * | 2019-08-19 | 2021-02-25 | 江苏汇先医药技术有限公司 | Microfluidic sample processing device |
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