CN108338025A - It is a kind of prevent miscellaneous bacteria infect device for raising seedlings and its application - Google Patents
It is a kind of prevent miscellaneous bacteria infect device for raising seedlings and its application Download PDFInfo
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- CN108338025A CN108338025A CN201810235317.1A CN201810235317A CN108338025A CN 108338025 A CN108338025 A CN 108338025A CN 201810235317 A CN201810235317 A CN 201810235317A CN 108338025 A CN108338025 A CN 108338025A
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- container
- plant growth
- raising seedlings
- seedling
- miscellaneous bacteria
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/029—Receptacles for seedlings
- A01G9/0293—Seed or shoot receptacles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/60—Cultivation rooms; Equipment therefor
- A01G18/64—Cultivation containers; Lids therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/021—Pots formed in one piece; Materials used therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Mycology (AREA)
- Cultivation Of Plants (AREA)
Abstract
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, which includes the container for plant growth being made of container bottoms and side, it is characterised in that：The side wall of the container for plant growth is equipped with air hole and inoculation hole, is fixedly connected with support ring on container for plant growth bottom surface, in support ring and is equipped with matrix bottom plate, is located at container for plant growth bottom surface and the container for plant growth side wall of matrix bottom plate is equipped with osculum.The device can also be equipped with shading and keep off the rain dust cover and water guider.Apparatus structure designed by the present invention is simple; it is easy to use; can not have to greenhouse facility or indoor conditions can cultivate Va Mycorrhiza Seedling under open-air condition; solves the problems, such as the series technique that Va Mycorrhiza Seedling scale, low cost, high quality are cultivated; the device for raising seedlings can also be used for arid area seedling nursery; it can greatly save with water, raising plant percent.
Description
Technical field
The invention belongs to a kind of device for raising seedlings more particularly to a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting.
Background technology
The cultivation of aseptic seedling and Va Mycorrhiza Seedling is because higher to environmental requirement, once there are non-purpose strains in environment, it is likely that
It infects to the nursery stock cultivated, therefore, the cultivation for aseptic seedling and Va Mycorrhiza Seedling currently mostly carries out in greenhouse, laboratory,
It cannot be cultivated under open-air condition because being easily contaminated.Wherein aseptic seedling refers to the nursery stock that root system is uninfected by mycorrhizal fungi, sterile
Seedling cultivation need to have following condition：Have a facility condition of keeping out wind and rain, seed, seedling medium, container for plant growth, watering pours water be both needed to by
Sterilizing, cultivating process avoid the brood body of the mycorrhizal fungi in wind, rainwater, soil from infecting.Cultivating aseptic seedling is to realize bacterium
The necessary first step of root fungus artificial infection and the steps necessary of artificial cultivation mycorhiza edible mushroom.Bolete, ferfas, delicious lactarius
One kind that edible mycorrhizal fungis are important in edible mushroom is waited, in forming exotrophic mycorrhiza on the root system of trees, it, which is absorbed, plants for its symbiosis
The photosynthate of object, while the nutrient supply trees that it is absorbed from soil utilize, and are symbiosis.Planting cultivation at present
Root edible mushroom common practice is that symbiosis sapling is first cultivated in the culture substrate of sterilizing, is with brood bodies such as mycelium or spores
Microbial inoculum is seeded to aseptic seedling root system, then by the symbiosis seedling after inoculation, in laboratory or after greenhouse culture for a period of time
It waits for that Va Mycorrhiza Seedling reaches requirement, is transplanted to production site fruiting afterwards after testing.
Aseptic seedling and Va Mycorrhiza Seedling nursery at present there are the problem of have：（1）Aseptic seedling and mycorrhiza fungi seeding cultivating can only be big in greenhouse
It is cultivated in canopy or laboratory, it is difficult to which large-scale production, production operation is not also strong, and facility input is larger, and production cost substantially increases
Add.（2）Aseptic seedling and Va Mycorrhiza Seedling large-scale production need to be implemented in nursery lot under the conditions of greenhouse facility, in nursery lot and around
Containing indigenous mycorrhizal fungi brood body in environment, such as spore, mycelium, easily infect miscellaneous bacteria in cultivating process, aseptic seedling and
Mycorrhiza fungi seeding cultivating success rate is not high.(3) nursery at present using it is wide for pot for growing seedlings, control root container, non-woven fabrics mesh bag etc. often
With container, the common disadvantage of these containers, which is bottom, a hole, and seedling root growth can be pierced by and with extending to garden in soil, to
Considerably increase indigenous VA Mycorrhizal Fungi infection aseptic seedling or the chance of Va Mycorrhiza Seedling.(4) common method of inoculation aseptic seedling has bacterium at present
Inoculation and punching filling microbial inoculum inoculation are broadcasted sowing in the inoculation of agent root dipping, microbial inoculum.Microbial inoculum root dipping and microbial inoculum broadcast sowing inocalation method and are both needed to transplanting nothing
Vaccine be easy to cause seedling root damage in transplanting seedling-slowing stage, and encountering the not in due course nursery stock easily death of weather leads to inoculation failure,
Although being influenced less on nursery stock using conventional punching filling microbial inoculum inocalation method, inoculation microbial inoculum is in direct contact machine with seedling root
Can be small, the growth speed of inoculation is very slow and is not easy long-term surviving, to reduce mycorhiza infection rate.(5) aseptic seedling is inoculated with
After microbial inoculum, three months after being especially just inoculated with, the water management of culture substrate was very difficult and is difficult to accurately grasp,
It often results in the reduction of mycorhiza infection rate or even nursery stock decays and death.(6) container of used culture Va Mycorrhiza Seedling is one at present
As container for plant growth, need to be placed under greenhouse or indoor conditions and use, fluorescent lamp is needed to provide light source, energy consumption is big, of high cost,
It is unfavorable for large-scale cultivation Va Mycorrhiza Seedling.Illumination is insufficient under the conditions of greenhouse, affects the photosynthesis and mycorhiza synthesis effect of nursery stock
Fruit.(6) when the nursery stock of Arbuscular Mycorrhizal Fungi bacterium is directly placed at using common container for plant growth under open-air condition, seedling medium is easy to lose
Water, the brood bodies such as non-purpose VA Mycorrhizal Fungi spore of nature can be infected by rainwater, wind or underground soil into matrix, to feel
Contaminate non-purpose VA Mycorrhizal Fungi（Such as Lasiosphaera fenzlii class, palpus false truffle）, easily pollute, purpose mycorrhiza fungi seeding cultivating caused to fail.
Invention content
For the above-mentioned problems in the prior art, the object of the present invention is to provide a kind of nursery for preventing miscellaneous bacteria from infecting
Device.Aseptic seedling and Va Mycorrhiza Seedling are cultivated using the device, Va Mycorrhiza Seedling root system part is made to be in relative closure item in full growth course
Growth and development under part, the non-more difficult intrusion seedling root of purpose fungus breeding body infects nursery stock, and purpose VA Mycorrhizal Fungi is by manually connecing
Kind after be easy to survive, and then can with scale, low cost cultivate qualified Va Mycorrhiza Seedling.
To achieve the above object, present invention employs following technical solutions：
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, which includes the container for plant growth being made of container bottoms and side, special
Sign is：The side wall of the container for plant growth is equipped with air hole and inoculation hole, and support ring is fixedly connected on container for plant growth bottom surface, supports
On ring and it is equipped with matrix bottom plate, the container for plant growth side wall between container for plant growth bottom surface and matrix bottom plate is equipped with draining
Hole.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：The support ring is by outer shroud and inner ring structure
At being respectively equipped with water export in outer shroud and inner ring；Water export on the outer shroud is arranged apart from osculum distalmost end；Institute
The water export stated in inner ring is arranged apart from osculum most proximal end.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：The matrix bottom plate by middle part waveform
Plate and the surface plate of periphery are constituted；The corrugated sheet is equipped with several leakage holes；The upper surface of the corrugated sheet is located at plane
The lower section of plate upper surface, and on the inside of inner ring.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：Inoculation on the container for plant growth side wall
Hole is circle, bore dia 1.5-2cm, and inoculation hole is arranged 3-6, is uniformly distributed in a ring, be arranged in container for plant growth on
Portion.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：Inoculation on the container for plant growth side wall
Hole is round, and bore dia 1.5-2cm, inoculation hole is arranged 5, is uniformly distributed, is arranged in the middle and upper part of container for plant growth in a ring；
Inoculation port lid is equipped in the inoculation hole；The inoculation port lid includes emulsion sheet and aerofoil profile buckle；Emulsion sheet（Size and
Shape is matched with inoculation hole, and aerofoil profile buckle is made of interior buckle and outer buckle.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：The air hole is bell mouth shape, opening
Outwardly.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：The device further includes for covering nursery
The shading of container is kept off the rain dust cover, and for the water guider to water filling in container.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：Shading dust cover upper end of keeping off the rain is set
There are elastic tying, lower end to be equipped with water outlet, tied rope is equipped on elastic tying；The shading keep off the rain dust cover using black and white it is anti-
Light membrane material is made, and white black outwardly is inwardly.
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, it is characterised in that：The water guider is by hollow bend pipe
It is constituted with the three foot guide supports for being secured to connection, the lower section of the three feet guide support is water guide groove.
Application of the device for raising seedlings on cultivating aseptic seedling and/or Va Mycorrhiza Seedling.
The present invention has the advantages that compared with prior art：（1）Apparatus structure designed by the present invention is simple, makes
With conveniently, exempts from the facilities such as greenhouse, non-purpose VA Mycorrhizal Fungi can be substantially reduced with large-scale cultivation purpose exotrophic mycorrhiza seedling（Such as Lasiosphaera fenzlii
Class, palpus false truffle etc.）Infection.（2）Container for plant growth is the hard plastics of black non transparent, plays support frame, black
Material provides dark situation for VA Mycorrhizal Fungi and root system.The interior sky for having 2-3cm between container for plant growth bottom surface and matrix bottom plate, avoids
It is in direct contact with nursery lot, prevents indigenous Va Mycorrhiza Seedling infection to seedling medium；Side wall, which is equipped with air hole, can meet bacterium
Root fungus and root system respiration demand.Side wall facilitates punching and purpose microbial inoculum to be inoculated with equipped with inoculation hole.（3）The shading dust cover that keeps off the rain is
Soft black and white reflective plastic film, inwardly, upper end ties up to nursery stock to black to white there are osculum and using springy tied rope outwardly
Rhizome portion avoids infecing non-purpose VA Mycorrhizal Fungi through the media such as rainwater or wind, while black and white reflective plastic film can be anti-by illumination
It is mapped to nursery stock leaf portion, increases photosynthesis, while reducing and influence dissipating for moisture in matrix on the direct projection of matrix due to illumination
Matrix of becoming estranged medium temperature spends the high injury to VA Mycorrhizal Fungi and root system.（4）The inoculation method of the present invention is using punching liquid filling body bacterium
Agent, and nutrient solution induction microbial inoculum continued growth is added, extend the survival period of strain, side can suitably be cut off using sharp card punch
Root hurts cortical tissue, is conducive to mycelia and infects root system, the inoculation method is unaffected to seedling growth, while can greatly improve
Mycorhiza infection rate.(5) on the one hand the water guider designed by the present invention plays the role of watering to Va Mycorrhiza Seedling, on the other hand rise
It is avoided and aqueous reflux to the keep off the rain effect of dust cover of support shading, the bend pipe of water guider connects water pipe for drip irrigation, i.e., using infiltration
The trickle irrigation of type is watered, and is irrigated once, and the long period without pouring, preferably maintains the moisture content of seedling medium, keeps nursery stock
The stability of root growth environment reduces because moisture changes the influence to VA Mycorrhizal Fungi and root system.（6）Present invention saves with
Water, the keep off the rain use of dust cover of shading reduce the moisture evaporation of seedling medium.（7）The more conventional container for plant growth of the present invention cultivates bacterium
Offspring, miscellaneous bacteria infection rate are substantially reduced, and purpose mycorhiza synthetic ratio greatly improves.The present invention can not have to greenhouse facility or indoor conditions
Va Mycorrhiza Seedling can be cultivated under open-air condition, solve Va Mycorrhiza Seedling scale, low cost, high quality cultivate series technique ask
Topic, the device for raising seedlings can also be used for arid area seedling nursery, can greatly save with water, raising plant percent.
Description of the drawings
Fig. 1, Fig. 2 are the cross-sectional view of container for plant growth in the present invention；
Fig. 3 is the overlooking structure diagram of container for plant growth in the present invention；
Fig. 4 does not load onto overlooking structure diagram when matrix bottom plate for container for plant growth in the present invention；
Fig. 5 is the structural schematic diagram of mesostroma bottom plate of the present invention；
Fig. 6 is a type of inoculation hole in the present invention；
The structural schematic diagram of port lid is inoculated with when Fig. 7 is matching circular inoculation hole in the present invention；
Fig. 8 is the structural schematic diagram of water guider in the present invention；
Fig. 9 is that the structural schematic diagram after water guider is loaded onto in container in the present invention；
Figure 10 is in the present invention to load onto water guider and shading is kept off the rain the schematic cross-sectional view after dust cover；
Figure 11 is suillus lutens-wet-land pine tree mycorrhizal morphology figure；
In figure：1- containers for plant growth；2- shadings are kept off the rain dust cover；3- water guiders；1-1- air holes；1-2- inoculation holes；1-21- connects
Connect bridge；1-3- support rings；1-31- outer shrouds；1-32- inner ring；1-4- osculums；1-5- matrix bottom plates；1-51- surface plates；1-52-
Corrugated sheet；1-53- leakage holes；The elastic tyings of 2-1；2-2- water outlets；2-3- tied ropes；3-1- bend pipes；Tri- foot guide supports of 3-2-；
3-3- water guide grooves.
Specific implementation mode
1-11 and specific implementation mode are described in further details the present invention with reference to the accompanying drawings of the specification.
Embodiment 1
A kind of device for raising seedlings for preventing miscellaneous bacteria from infecting designed by the present invention, educates including at least what is be made of container bottoms and side
Seedling container 1.Unlike existing container for plant growth, the side wall of the container for plant growth 1 is equipped with air hole 1-1 and inoculation hole 1-2, educates
It is fixedly connected with support ring 1-3 on 1 bottom surface of seedling container, in support ring and is equipped with matrix bottom plate 1-5, be located at container for plant growth bottom surface
It is equipped with osculum 1-4 with the container for plant growth side wall of matrix bottom plate 1-5.In order to preferably prevent extraneous bacterium from osculum 1-4
Into, the support ring 1-3 designed by the present invention is made of two rings of outer shroud 1-31 and inner ring 1-32 for being respectively provided with water export,
Water export setting on outer shroud 1-31 is located at osculum and the container for plant growth bottom surface center of circle apart from osculum 1-4 distalmost end
2 lines extended line on；Water export on the inner ring 1-31 is arranged apart from osculum 1-4 most proximal ends；It is located at
Two lines and inner ring intersection of the osculum with the container for plant growth bottom surface center of circle；The matrix bottom plate 1-5 by middle part corrugated sheet
1-52 and the surface plate 1-51 of periphery are constituted；The corrugated sheet 1-52 is equipped with several leakage holes 1-53；The corrugated sheet 1-
52 upper surface is located at the lower section of the upper surfaces surface plate 5-1, and on the inside of inner ring 1-32, and the purpose of this design is maximum journey
Completely cut off with external environment on degree, while realizing moisture extra in matrix from the leakage hole 1-53 outflows on corrugated sheet, through inner ring
1-31 flows out into outer shroud 1-31, finally on the outside of osculum 1-4 discharge containers for plant growth.Container for plant growth bottom surface and matrix bottom plate
Between be preferably provided with the interior sky of 2-3cm, i.e. the distance between container for plant growth bottom surface and matrix bottom plate is 2-3cm.Container for plant growth
Structure see Fig. 1-5（Wherein, Fig. 1 is longitudinal profile schematic diagram of the container for plant growth at non-osculum；Fig. 2 is that container for plant growth exists
Longitudinal profile schematic diagram at osculum）.The design, which is more advantageous to, maintains metastable water content in matrix.
Inoculation hole 1-3 on container for plant growth side wall can be diversified forms, can be the structure of integral type, see Fig. 6, the knot
Structure is that the 2-4 fan platform holes for being located at concentric circles are preset on container side wall, is 4 in Fig. 6, fan platform is cut with scissors when inoculation
Between connecting bridge 1-21, only leave a connecting bridge and do not cut, will inoculation bore region outwardly open, punch and access microbial inoculum,
Then inoculation bore region will be outwardly opened to restore, covered on container side wall, sealed and fixed with sealed membrane.Certainly, inoculation hole is also
Reserved circular hole is can be designed as, when being designed as round hole, bore dia is preferably 1.5-2cm, and inoculation hole is arranged 3-6, is in
Annular is uniformly distributed, and is arranged in the middle and upper part of container for plant growth, is equipped with inoculation port lid at this time；The inoculation port lid includes covering
Piece and aerofoil profile buckle（See Fig. 7）；The size and shape of emulsion sheet is matched with inoculation hole, and aerofoil profile buckle is by interior buckle and outer buckle structure
At, by it is inside and outside buckle will inoculation port lid be fixed at the inoculation hole of container for plant growth side wall.Air hole 1 set by the present invention can
Think circle, certainly preferably bell mouth shape, opening is outwardly.Rigid plastics material of the container for plant growth preferably with black non transparent
Material, plays support frame, black material provides dark situation for VA Mycorrhizal Fungi and root system.The design of the container for plant growth can be significantly
Reduce the miscellaneous bacteria infection from container bottom.
In order to avoid miscellaneous bacteria infection aseptic seedling or Va Mycorrhiza Seedling in air and rainwater, the present invention devises shading and keeps off the rain dust cover
2, it is made preferably with black and white reflecting film material, white black outwardly is inwardly.The upper end is equipped with elastic tying 2-1, and lower end is set
Have and is equipped with tied rope 2-3 on water outlet 2-2, elastic tying 2-1；Tied rope 2-3 is preferably stretch cord, ties up to nursery stock rhizome portion,
It is elastic suitable for avoiding loose rainwater from flowing into, also tension is avoided to be unfavorable for plant growth.Because cover body wraps container for plant growth, in nursery
There are gaps for container base side（That is water outlet 2-2）Water supply and sewage avoids root system and seedling medium and is in direct contact with nursery lot,
Indigenous Va Mycorrhiza Seedling travels in seedling medium.The keep off the rain design of dust cover of shading is avoided through rainwater, wind and nursery lot soil etc.
Medium infects non-purpose VA Mycorrhizal Fungi, while illumination can be reflected into nursery stock leaf portion by black and white reflective plastic film, increase photosynthetic work
With, at the same reduce the direct projection of matrix is influenced due to illumination moisture in matrix scatter and disappear and matrix medium temperature spends height to mycorhiza
The injury of bacterium and root system.The shading dust cover that keeps off the rain can directly be made of one piece of black and white reflective membrane, and reflective membrane or so is rolled over
Folded to overlap into cylindrical shape, upper end is fixed on seedling mu rhizome portion with tied rope, and lower end covers container bottom and leaves latasuture to drain.
It waters for convenience to aseptic seedling or Va Mycorrhiza Seedling, the present invention adds water guider 3（See Fig. 8）, water guider 3 is in
Empty bend pipe 3-1 and the three foot guide support 3-2 for being secured to connect are constituted, and the lower section of the three feet guide support 3-2 is to lead
Water groove 3-3.In use, bend pipe 3-1 inlet ends are connected water pipe for drip irrigation, that is, the trickle irrigation of osmosis type is used to water, using oozing
Ability of swimming irrigation flow is slower, is influenced on root system and hyphal development smaller.It irrigates once, the long period without pouring, preferably ties up
Held the moisture content of seedling medium, kept the stability of seedling root growing environment, reduce because of moisture variation to VA Mycorrhizal Fungi and
The influence of root system, three months after being especially just inoculated with.On the one hand the structure plays the role of watering to Va Mycorrhiza Seedling, on the other hand
Play the role of that the shading dust cover that keeps off the rain is supported to prevent rainwater from blowing back into container.
Mycorrhiza fungi seeding cultivating is carried out using above-mentioned apparatus, key step is as follows：
1）Cultivate aseptic seedling：Vegetable seeds for nursery drains away the water after fully being impregnated with tap water, removes seed inferior, uses
0.1% potassium permanganate impregnates 1 hour and carries out surface sterilization, and educating equipped with seedling medium is seeded in after aseptic water washing three times
It in seedling container, is cultivated under soil humidity 60-70%, sterile water pours；Seedling medium need to sterilize 1h in 121 DEG C, 0.1MPa.It should
Stage cultivates in greenhouse, greenhouse or laboratory.Certainly, long in aseptic seedling in greenhouse, greenhouse or laboratory when the anxiety of place
To certain altitude, the shading dust cover that keeps off the rain can also be put on and removed to outside scenery；
2）It is prepared by microbial inoculum：It is inoculum or using spore as the microbial inoculum of inoculum to prepare using mycelium；Microbial inoculum can be solid fungicide
Or liquid bacterial agent, wherein liquid bacterial agent are better.It is as follows as the bacterial preparation process of inoculum using spore：Take the fresh of maturation
Fructification is ground into the particle of soya bean size, is positioned in sealing container, cultivates 24 hours at 25 DEG C, by volume fructification
Particle：Nutrient solution=1：Nutrient solution is added in 1.5 ratio in a reservoir, obtains bacterium solution after mixing；Add in per 1000mL bacterium solutions
Enter 3-5g sodium alginates, obtains microbial inoculum after mixing；It is as follows as the bacterial preparation process of inoculum using mycelium：Mycelium passes through
Liquid expand it is numerous after, obtain bacterium ball after filtering, bacterium ball and nutrient solution by volume 1:1 is uniformly mixed, every 1000 milliliters of bacterium ball nutrition
Liquid adds 3-5g sodium alginates, stirs evenly；
3）Aseptic seedling is inoculated with：After aseptic seedling has lateral root development, inoculation port lid 1-6 is removed, at the inoculation hole 1-2 of container for plant growth
Microbial inoculum is accessed after being punched using card punch, is fixed at inoculation hole by inside and outside buckle 1-7 by port lid 1-6 is inoculated with, i.e., to inoculation
Hole is sealed and is fixed on container for plant growth, and then direction stromal surface pours into a mould nutrient solution from container；And then, in nursery
Sterilized seedling medium is added in container 1 again until filling container for plant growth and slightly at steamed bun shape, then again in seedling medium
Upper storing water guider 3, it is desirable that the stem of bend pipe 3-1 and aseptic seedling reclines, then on container for plant growth 1 cover shading keep off the rain it is dust-proof
Cover 2, the aerial part of aseptic seedling and the bend pipe 3-1 of water guider 3 keep off the rain dust cover 2 across elastic tying 2-1 positioned at shading
Outside, fasten tied rope 2-3 at elastic tying 2-1 with tied rope 2-3（See Fig. 9-10）.Card punch of the present invention is best
For following design：Card punch is constituted by punching end and handle end two parts, and punching end is flat sharp contract type（Like axe）, a length of
5-6cm, width 1.5-1.8cm, handle end are cylinder, a length of 8-12cm, a diameter of 1.5-1.8cm；
5）The cultivation of Va Mycorrhiza Seedling：Aseptic seedling after inoculation removes greenhouse or greenhouse, is placed under natural environmental condition and cultivates, by leading
3 entrance of water installations injects sterile water；The sterile water is 15m underground water below from depth.
It is important to note that the present apparatus can be used for Aseptic Seedling Growth to outdoor culture after a certain size, at this time
Shading is loaded onto to keep off the rain dust cover 2 and water guider 3.
Device designed by the present invention is suitable for the symbiosis of such as bolete, delicious lactarius, ferfas edible mycorrhizal fungi type
Seedling culture, main sport technique segment are：（1）Select symbiosis seeds appropriate, such as brown ring viscous according to the characteristic of mycorhiza edible mushroom
Lid bolete and the symbiosis seeds of delicious lactarius are wet-land pine tree, masson pine etc.；The symbiosis host plant of ferfas is pecan tree, plate
Chestnut, pinus yunnanensis etc.；（2）Cultivating aseptic seedling container uses container for plant growth of the present invention, matrix that high-temperature sterilization, water source is used to come from 15m
Following underground water, sowing to reach inoculation require during in greenhouse or indoor culture；（3）It waits for that aseptic seedling reaches inoculation and requires, makes
Punch filling microbial inoculum with card punch, addition nutrient solution induces microbial inoculum continued growth, increase root system and strain touch opportunity and when
Between；（4）Using the culture vessel of the present invention, the cultural method after proper use of inoculation and operating procedure；（5）The cultivating aseptic seedling phase
Cultivate indoors or in greenhouse, it is to be seeded after capping shading dust cover and the water guider full exposure under open-air condition of keeping off the rain train
It educates.
Embodiment 2
The wet-land pine tree symbiosis seedling of suillus lutens is inoculated with and cultivated using the device in embodiment 1, and method is as follows：
1, prepared by microbial inoculum：
1）Strain tissue detaches：Suillus lutens fructification is acquired in Lishui City Liandu District west of a city village all kinds of fruits garden, takes back reality
It tests room and carries out tissue separation, take cap and stem cohesive position mung bean size tissue, place and cultivate one on special solid culture medium
Picking mycelia purifying culture is spare after a week.The special solid culture medium prescription is：Potato 100g, which boils, filters to take juice,
Glucose 25g, 1.5 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate, ammonium tartrate 1 g, 1% ironic citrate 0.5ml, 0.1% zinc sulfate
2.5ml, vitamin B1 10mg, agar 15g add water to be settled to 1000ml.
2）Strain expands numerous：Cultured strain is taken, 1cm is cut with scalpel23 pieces of strain block, which is put into liquid shaking bottle, to be trained
It supports, Liquid Culture based formulas：Potato 100g, which boils, filters to take juice, glucose 25g, 1.5 g of potassium dihydrogen phosphate, 1 g of magnesium sulfate,
Ammonium tartrate 1 g, 1% ironic citrate 0.5ml, 0.1% zinc sulfate 2.5ml, vitamin B1 10mg add water to be settled to 1000ml.It adopts
With 500ml shaking flasks, liquid amount 250ml, it is spare to cultivate 15d for 25 DEG C, rotating speed 130r/min of cultivation temperature.
3）It is prepared by liquid bacterial agent：Through liquid expand it is numerous after the filtered bacterium ball of mycelium and nutrient solution（Formula is the same as step 2
Fluid nutrient medium）By volume 1:1, every 1000 milliliters plus 3-5g of sodium alginate, which is mixed thoroughly, is made mycelium liquid microbial inoculum.
2, cultivating aseptic seedling
Wet-land pine tree (pinus elliottiiEngelm) seed drains away the water after fully being impregnated with tap water, inferior kind of removal
Son impregnates 1 hour with 0.1% potassium permanganate and carries out surface sterilization, is sowed at equipped with seedling medium after aseptic water washing 3 times
It in container for plant growth, is cultivated under soil humidity 60-70%, sterile water pours；Seedling medium need to sterilize 1h in 121 DEG C, 0.1MPa.
Cultivating aseptic seedling season is 8-9 months.It is cultivated in greenhouse or greenhouse.Seedling medium formula is vermiculite：Soddy soil：Perlite=
3:1:1（Volume ratio）, natural ph.
3, it is inoculated with
It is inoculated in month autumn and winter 11-12, inoculation method is：Using sharp card punch, downward oblique cutting enters at inoculation hole, punching
Depth is 5-10cm, is sealed with inoculation port lid after being then injected into the holes liquid bacterial agent 5ml/, then portion is uniform to matrix from container
It pours and spreads nutrient solution about 300-500ml.Seedling medium is added again and is earthed up into steamed bun shape.
4, mycorrhiza fungi seeding cultivating
The aseptic seedling being inoculated with is installed into water guider and shading is kept off the rain dust cover, is placed under open-air condition and cultivates.With ground
Lower phreatic water is water source, is not watered in 2 week after inoculation, and backsight soil moisture content situation pours moisture, generally keeps soil
Water content is in 60-70%, and culture 6-12 months, the slash pine seedlings radicula surface mycelia of inoculation covers to be formed, and mycorrhizal morphology is y-bend
Shape, white, are shown in Figure 11.
Miscellaneous bacteria source air spore, garden ground indigenous VA Mycorrhizal Fungi, rainwater miospore etc., wherein since root system is given birth to downwards in soil
In long with extending to garden soil, and garden be commonly present Lasiosphaera fenzlii class in soil, must the VA Mycorrhizal Fungis such as false truffle, therefore easily infect.Through adjusting
Look into, under open-air condition garden nursery mycorhiza infection conditions be shown in Table 1.The results show that using general common vessel nursery on the market,
Miscellaneous bacteria infection rate is up to 38.6%, and Lasiosphaera fenzlii class infection rate is 28.1%.And the device miscellaneous bacteria infection rate used in this patent is 1.1%, horse
Vigorous class infection rate is 0.The more conventional cultivation container Va Mycorrhiza Seedling of the present invention, greatly reduces the dip dyeing of Lasiosphaera fenzlii class and miscellaneous bacteria, to real
Existing purpose mycorhiza synthetic ratio greatly improves, and the suillus lutens Va Mycorrhiza Seedling that high quality is produced for scale, low cost carries
Technical guarantee is supplied.
Ground nursery mycorhiza infection conditions table in garden under 1 open-air condition of table
Claims (10)
1. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting, which includes the container for plant growth being made of container bottoms and side（1）,
It is characterized in that：The container for plant growth（1）Side wall be equipped with air hole（1-1）And inoculation hole（1-2）, container for plant growth（1）Bottom surface
On be fixedly connected with support ring（1-3）, in support ring and it is equipped with matrix bottom plate（1-5）, it is located at container for plant growth bottom surface and matrix
Bottom plate（1-5）Between container for plant growth side wall be equipped with osculum（1-4）.
2. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 1, it is characterised in that：The support ring（1-
3）By outer shroud（1-31）And inner ring（1-32）It constitutes, outer shroud（1-31）And inner ring（1-32）On be respectively equipped with water export；It is described
Outer shroud（1-31）On water export be arranged apart from osculum（1-4）Distalmost end；The inner ring（1-31）On water export
It is arranged apart from osculum（1-4）Most proximal end.
3. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 1, it is characterised in that：The matrix bottom plate
（1-5）By the corrugated sheet at middle part（1-52）With the surface plate of periphery（1-51）It constitutes；The corrugated sheet（1-52）It is equipped with several
A leakage hole（1-53）；The corrugated sheet（1-52）Upper surface be located at surface plate（5-1）The lower section of upper surface, and positioned at interior
Ring（1-32）Inside.
4. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 1, it is characterised in that：The container for plant growth side
Inoculation hole on wall（1-3）For circle, bore dia 1.5-2cm, inoculation hole is arranged 3-6, is uniformly distributed in a ring, and setting exists
The middle and upper part of container for plant growth.
5. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 1, it is characterised in that：The container for plant growth side
Inoculation hole on wall（1-2）For circle, bore dia 1.5-2cm, inoculation hole is arranged 5, is uniformly distributed in a ring, and setting is being educated
The middle and upper part of seedling container；The inoculation hole（1-2）On be equipped with inoculation port lid；The inoculation port lid includes emulsion sheet and aerofoil profile
Buckle；The size and shape of emulsion sheet is matched with inoculation hole, and aerofoil profile buckle is made of interior buckle and outer buckle.
6. according to a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting described in claim 1, it is characterised in that：The air hole
（1）For bell mouth shape, opening is outwardly.
7. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 1, it is characterised in that：The device further includes using
It keeps off the rain dust cover in the shading of covering container for plant growth（2）, and for the water guider to water filling in container for plant growth（3）.
8. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 7, it is characterised in that：The shading is kept off the rain anti-
Dust hood（2）Upper end is equipped with elastic tying（2-1）, lower end is equipped with water outlet（2-2）, elastic tying（2-1）On be equipped with tied rope
（2-3）；The shading is kept off the rain dust cover（2）It is made using black and white reflecting film material, white black outwardly is inwardly.
9. a kind of device for raising seedlings for preventing miscellaneous bacteria from infecting according to claim 7, it is characterised in that：The water guider
（3）By hollow bend pipe（3-1）With the three foot guide supports for being secured to connection（3-2）It constitutes, the three feet guide support（3-
2）Lower section be water guide groove（3-3）.
10. device for raising seedlings the answering in cultivation aseptic seedling and/or Va Mycorrhiza Seedling according to any one of claim 1-9
With.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109496773A (en) * | 2018-12-26 | 2019-03-22 | 云南省林业科学院漾濞核桃研究院 | Apocarya Light media small container grafting Raining season wooding method |
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| CN108338025B (en) | 2023-11-14 |
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