CN108308071A - A kind of efficient industrialized propagation method of Chinese snake head - Google Patents
A kind of efficient industrialized propagation method of Chinese snake head Download PDFInfo
- Publication number
- CN108308071A CN108308071A CN201810021427.8A CN201810021427A CN108308071A CN 108308071 A CN108308071 A CN 108308071A CN 201810021427 A CN201810021427 A CN 201810021427A CN 108308071 A CN108308071 A CN 108308071A
- Authority
- CN
- China
- Prior art keywords
- loach
- parts
- snake head
- propagation method
- chinese snake
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 46
- 241001597062 Channa argus Species 0.000 title claims abstract description 37
- 241000252185 Cobitidae Species 0.000 claims abstract description 130
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 55
- 230000012447 hatching Effects 0.000 claims abstract description 23
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 20
- 239000001301 oxygen Substances 0.000 claims abstract description 20
- 238000009395 breeding Methods 0.000 claims abstract description 18
- 230000001488 breeding effect Effects 0.000 claims abstract description 18
- 238000002347 injection Methods 0.000 claims abstract description 16
- 239000007924 injection Substances 0.000 claims abstract description 16
- 230000009027 insemination Effects 0.000 claims abstract description 6
- 230000032696 parturition Effects 0.000 claims abstract description 6
- 235000013372 meat Nutrition 0.000 claims description 32
- 239000000284 extract Substances 0.000 claims description 30
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 28
- 235000013601 eggs Nutrition 0.000 claims description 22
- 235000013339 cereals Nutrition 0.000 claims description 20
- 235000019733 Fish meal Nutrition 0.000 claims description 17
- 239000004467 fishmeal Substances 0.000 claims description 17
- 102000002322 Egg Proteins Human genes 0.000 claims description 15
- 108010000912 Egg Proteins Proteins 0.000 claims description 15
- 229940079593 drug Drugs 0.000 claims description 15
- 239000003814 drug Substances 0.000 claims description 15
- 230000002196 ecbolic effect Effects 0.000 claims description 15
- 210000004681 ovum Anatomy 0.000 claims description 15
- 241000251468 Actinopterygii Species 0.000 claims description 11
- 241000238366 Cephalopoda Species 0.000 claims description 11
- 210000004185 liver Anatomy 0.000 claims description 11
- 239000003963 antioxidant agent Substances 0.000 claims description 10
- 230000003078 antioxidant effect Effects 0.000 claims description 10
- 235000006708 antioxidants Nutrition 0.000 claims description 10
- 239000002434 gonadorelin derivative Substances 0.000 claims description 10
- 239000002504 physiological saline solution Substances 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 10
- 238000005292 vacuum distillation Methods 0.000 claims description 10
- 239000002994 raw material Substances 0.000 claims description 9
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 235000013312 flour Nutrition 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 244000068988 Glycine max Species 0.000 claims description 7
- 235000010469 Glycine max Nutrition 0.000 claims description 7
- 241000209140 Triticum Species 0.000 claims description 7
- 235000021307 Triticum Nutrition 0.000 claims description 7
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 7
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 7
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 7
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims description 7
- 229960001231 choline Drugs 0.000 claims description 7
- 238000000227 grinding Methods 0.000 claims description 7
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 229940088594 vitamin Drugs 0.000 claims description 7
- 239000011782 vitamin Substances 0.000 claims description 7
- 235000013343 vitamin Nutrition 0.000 claims description 7
- 229930003231 vitamin Natural products 0.000 claims description 7
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 7
- 235000010352 sodium erythorbate Nutrition 0.000 claims description 6
- 208000027418 Wounds and injury Diseases 0.000 claims description 5
- 210000001015 abdomen Anatomy 0.000 claims description 5
- 239000004927 clay Substances 0.000 claims description 5
- 201000010099 disease Diseases 0.000 claims description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- 210000003746 feather Anatomy 0.000 claims description 5
- 230000009969 flowable effect Effects 0.000 claims description 5
- 230000036541 health Effects 0.000 claims description 5
- 208000014674 injury Diseases 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 210000003205 muscle Anatomy 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- CIWBSHSKHKDKBQ-DUZGATOHSA-N D-isoascorbic acid Chemical compound OC[C@@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-DUZGATOHSA-N 0.000 claims description 4
- 239000011786 L-ascorbyl-6-palmitate Substances 0.000 claims description 4
- 229960005070 ascorbic acid Drugs 0.000 claims description 4
- QAQJMLQRFWZOBN-LAUBAEHRSA-N L-ascorbyl-6-palmitate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](O)[C@H]1OC(=O)C(O)=C1O QAQJMLQRFWZOBN-LAUBAEHRSA-N 0.000 claims description 3
- 235000010323 ascorbic acid Nutrition 0.000 claims description 3
- 239000011668 ascorbic acid Substances 0.000 claims description 3
- 235000010385 ascorbyl palmitate Nutrition 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium erythorbate Chemical compound [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 claims description 2
- 210000001136 chorion Anatomy 0.000 claims 1
- 210000004907 gland Anatomy 0.000 claims 1
- 229940088597 hormone Drugs 0.000 claims 1
- 239000003668 hormone analog Substances 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 230000001568 sexual effect Effects 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 6
- 230000000052 comparative effect Effects 0.000 description 7
- 230000008859 change Effects 0.000 description 5
- 238000004080 punching Methods 0.000 description 5
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 4
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 4
- 229940015047 chorionic gonadotropin Drugs 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 230000026109 gonad development Effects 0.000 description 3
- 230000004899 motility Effects 0.000 description 2
- 241001417978 Channidae Species 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 235000000072 L-ascorbyl-6-palmitate Nutrition 0.000 description 1
- 241001275944 Misgurnus anguillicaudatus Species 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002860 competitive effect Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Marine Sciences & Fisheries (AREA)
- Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Food Science & Technology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of efficient industrialized propagation methods of Chinese snake head, belong to loach reproduction technique field.The efficient industrialized propagation method of the Chinese snake head includes：1) close loach is selected；2) cultivation of close loach；3) injection is hastened parturition；4) artificial insemination；5) hatching and 6) nursery.The efficient industrialized propagation method of the present invention by launching the male and female parent loach of proper proportion, breeding density appropriate, using the water flow velocity and oxygen flow velocity etc. in homemade cultivation feed and control hatching cylinder, the final reproduction speed for making the present invention substantially increase Chinese snake head and hatching survival rate, are conducive to efficient, the large-scale breeding of Chinese snake head.
Description
Technical field
The present invention relates to loach reproduction technique fields, and in particular to a kind of efficient industrialized propagation method of Chinese snake head.
Background technology
Chinese snake head belongs to one kind in Cobitidae pair loach genus Misgurnus auguillicaudatus, is commonly called as big loach.This loach has had
Artificial breeding history for many years is the loach that China most adapts to artificial breeding environment.The loach is on Guangdong, Guangxi, Fujian and other places
Breeding scale confirm that the seedling of loach breeding, up to the specification of 100 grams or so of single, is current more competitive in current year
Loach kind.It is full of nutrition because the fish speed of growth is fast, fine and tender taste, delicious flavour since introducing China, it is to nourish in winter
One of preferred fingerling, therefore culturist is more and more, and economic benefit is higher.
However in the propagation method of the Chinese snake head used at present, the reproduction speed of Chinese snake head is slower, and hatching survives
Rate is not very high, therefore in the breeding of Chinese snake head, still needs to explore more preferably propagation method, to improve the numerous of Chinese snake head
Grow speed and hatching survival rate.
Invention content
In view of the above-mentioned problems, the object of the present invention is to provide a kind of efficient industrialized propagation method of Chinese snake head, the height
Effect industrialized propagation method substantially increases the reproduction speed and hatching survival rate of Chinese snake head, is conducive to the height of Chinese snake head
Effect, large-scale breeding.
To achieve the goals above, the technical solution adopted by the present invention is：
A kind of efficient industrialized propagation method of Chinese snake head, includes the following steps：
(1) close loach is selected
Select that physical strong, body colour is bright, the bodily form is proper, the close loach of the complete health of energetic, disease-free and injury-free, fin ray, it is male
Close loach requires body long 10-18cm, weight 10-25g, female parent's loach body long 12-25cm, weight 30-45g；
(2) cultivation of close loach
Female parent loach and male close loach are separately put into culture pond and carry out bath cultivation, culture pond requires that water source is abundant, water quality
Pure and fresh pollution-free, it is 25-30 days to cultivate the time, and bottom of pond is laid with 15-20 centimetres of clay, the ratio of the female parent loach of dispensing and male close loach
Example is 1：2-4, breeding density are to put 120-200Kg in a suitable place to breed per acre, feed close loach daily and cultivate feed, feed daily twice, every time
Feeding volume is the 5%-8% of close loach weight；
The cultivation feed is mainly composed of the following raw materials in parts by weight：It is 50-70 parts of fish meal, 25-35 parts of fermented bean dregs, small
20-30 parts of wheat flour, 15-20 parts of soybean stalk powder, 10-15 parts of squid liver paste, 5-10 parts of clam meat extract, calcium dihydrogen phosphate 2-10
Part, 1-5 parts of choline, 1-5 parts of vitamin and 0.5-1.5 parts of antioxidant；
(3) injection is hastened parturition
Oxytocic drug is injected for female parent loach and male close loach, injecting method is a muscle of back shot, and oxytocic drug is suede
The human chorionic gonadtropin injection volume of Chorionic Gonadotropin and luteinizing hormone-releasing hormone analog, female parent loach is
The injection volume of 15-20IU/g, luteinizing hormone-releasing hormone analog are 0.1-0.3ug/g, and the dosage of male parent loach halves, water
Temperature is maintained at 25-30 DEG C；
(4) artificial insemination
Oxytocic drug after 9-12 hour is injected, light pressure female parent loach abdomen squeezes into ovum grain in vessel, then by the sperm of male loach
It squeezes out into same vessel, the physiological saline of a concentration of 0.35%-0.5% is then added, is gently mixed using feather or greenery,
So that ovum grain and sperm are sufficiently mixed, are fertilized, obtains fertilized eggs；
(5) hatch
Fertilized eggs are gently sprinkled upon on fish nest, is transferred to during water is sufficient and the flowable hatchery of water and hatches, while toward incubating
Change and be passed through oxygen in pond, wherein the flow velocity of water is 3-4m/min, and the flow velocity of oxygen is 0.2-0.6m/s；Water temperature is 23-25 DEG C, is incubated
To get loach seedlings after changing 28-32 hours；
(6) nursery
Loach seedlings after hatching, which continue to stay in former hatchery, to be cultivated, and the depth of water is maintained at 20-40 centimetres, every square metre of density with
1000-2000 tails are advisable；And the soft-boiled eggs Huang or fish meal of grinding are fed for loach seedlings, it is three times per day to feed number；It cultivates 3-5 days
Afterwards, loach seedlings are gone into the raising of seedling pond.
Preferably, it also carries out disinfection to culture pond before close loach being launched culture pond in step (2).
Preferably, in order to promote the gonad development of close loach, of the invention cultivates feed using the self-control feeding rich in protein
Material, the cultivation feed are mainly composed of the following raw materials in parts by weight：60 parts of fish meal, 30 parts of fermented bean dregs, 25 parts of wheat flour,
18 parts of soybean stalk powder, 12 parts of squid liver paste, 8 parts of clam meat extract, 5 parts of calcium dihydrogen phosphate, 3 parts of choline, 3 parts of vitamin and antioxygen
1 part of agent.
Preferably, the antioxidant is ascorbic acid, ascorbyl palmitate, arabo-ascorbic acid and arabo-ascorbic acid
One or more of sodium.
Preferably, the extracting method of the clam meat extract be take fresh clam meat to clean, drain after rub, be put into equipped with fourth
It extracts 30-60 minutes, extracts 2 to 4 times in the extractor of alcoholic solvent, merge extracting solution, then butanol is removed by vacuum distillation, i.e.,
Obtain clam meat extract.
Preferably, the weight ratio of the butanol solvent and the clam meat is 1:0.05-0.2.
Preferably, the temperature of the extraction is 2-8 DEG C, and the temperature of the vacuum distillation is 3-10 DEG C.
Preferably, step (4) the ovum grain and the molecular mixture of essence and the physiological saline and weight ratio be 1：
50-60。
Preferably, the soft-boiled eggs Huang of step (6) described grinding or the grain size of fish meal are 45-75 microns.
In conclusion by adopting the above-described technical solution, beneficial effects of the present invention are：
(1) by using the efficient industrialized propagation method of the present invention, including the male and female parent loach, appropriate of proper proportion is launched
Breeding density, using the water flow velocity and oxygen flow velocity etc. in homemade cultivation feed and control hatching cylinder, as cultivated in feed
Contain higher protein in the raw materials such as fish meal, squid liver paste, clam meat extract, this promotes the gonad development of close loach significantly, simultaneously
Water flow velocity and oxygen flow velocity appropriate not only ensure that the abundance of the oxygen content in the pure and fresh of water quality, water, can also prevent fertilized eggs from fish
It is fallen by punching on nest, so that the present invention is substantially increased the reproduction speed and hatching survival rate of Chinese snake head, be conducive to Taiwan mud
Efficient, the large-scale breeding of loach.
(2) present invention can not only provide higher by adding squid liver paste and clam meat extract, squid liver paste in cultivating feed
Protein content, and be alternatively arranged as the phagostimulant of close loach feed, and clam meat extract is by fully being extracted its albumen by clam meat
After matter, for the substance rich in protein, the two is added in the major ingredients such as fish meal, dregs of beans, flour, not only substantially increases cultivation
The protein content of feed further promotes the appetite of close loach, to substantially increase Chinese snake head reproduction speed and hatching at
Motility rate.
Specific implementation mode
The following is a clear and complete description of the technical scheme in the embodiments of the invention, it is clear that described embodiment
Only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment 1
A kind of efficient industrialized propagation method of Chinese snake head, includes the following steps：
(1) close loach is selected
Select that physical strong, body colour is bright, the bodily form is proper, the close loach of the complete health of energetic, disease-free and injury-free, fin ray, it is male
Close loach requires body long 10-15cm, weight 10-15g, female parent's loach body long 12-18cm, weight 30-35g.
(2) cultivation of close loach
After carrying out disinfection to culture pond, female parent loach and male close loach are separately put into culture pond and carry out bath cultivation, is cultivated
Pond requirement water source is abundant, water quality is pure and fresh pollution-free, and it is 25 days to cultivate the time, and bottom of pond is laid with 15 centimetres of clay, the female parent of dispensing
The ratio of loach and male close loach is 1：2, breeding density is to put 120Kg in a suitable place to breed per acre, feeds close loach daily and cultivates feed, feeds two daily
Secondary, each feeding volume is the 5% of close loach weight.
The cultivation feed is mainly composed of the following raw materials in parts by weight：50 parts of fish meal, 25 parts of fermented bean dregs, wheat flour
20 parts, 15 parts of soybean stalk powder, 10 parts of squid liver paste, 5 parts of clam meat extract, 2 parts of calcium dihydrogen phosphate, 1 part of choline, 1 part of vitamin
With 0.5 part of antioxidant.Wherein, the antioxidant is ascorbic acid.
The extracting method of the clam meat extract be take fresh clam meat to clean, drain after rub, be put into equipped with butanol solvent
Extractor in extract 30 minutes, extract 4 times, merge extracting solution, then by vacuum distillation remove butanol to get clam meat extract
Object.Wherein, the weight ratio of butanol solvent and clam meat is 1:0.05, the temperature of extraction is 2 DEG C, and the temperature of vacuum distillation is 3 DEG C.
(3) injection is hastened parturition
Oxytocic drug is injected for female parent loach and male close loach, injecting method is a muscle of back shot, and oxytocic drug is suede
The human chorionic gonadtropin injection volume of Chorionic Gonadotropin and luteinizing hormone-releasing hormone analog, female parent loach is
The injection volume of 15IU/g, luteinizing hormone-releasing hormone analog are 0.3ug/g, and the dosage of male parent loach halves, and water temperature is kept
At 25 DEG C.
(4) artificial insemination
Oxytocic drug is injected after 9 hours, it is light that female parent loach abdomen is pressed to squeeze into ovum grain in vessel, then the sperm of male loach is squeezed out
To a concentration of 0.35% physiological saline, ovum grain and the molecular mixture of essence in same vessel, is then added and is added
Physiological saline and weight ratio be 1：50, then feather or greenery is used to be gently mixed, so that ovum grain and sperm is sufficiently mixed, by
Essence obtains fertilized eggs.
(5) hatch
Fertilized eggs are gently sprinkled upon on fish nest, is transferred to during water is sufficient and the flowable hatchery of water and hatches, while toward incubating
Change and be passed through oxygen in pond, wherein the flow velocity of water is 3m/min, and the flow velocity of oxygen is 0.6m/s, to prevent fertilized eggs from quilt on fish nest
Punching is fallen, while ensureing the pure and fresh clean of water quality, the abundance of water oxygen；Water temperature is 23 DEG C, to get loach seedlings after hatching 28 hours.
(6) nursery
Loach seedlings after hatching, which continue to stay in former hatchery, to be cultivated, and the depth of water is maintained at 20-30 centimetres, every square metre of density with
1000-1500 tails are advisable；And be that soft-boiled eggs that grain size that loach seedlings feed grinding is 45 microns are yellow or fish meal, it is one day three to feed number
It is secondary；After cultivating 3-5 days, loach seedlings are gone into the raising of seedling pond.
Embodiment 2
A kind of efficient industrialized propagation method of Chinese snake head, includes the following steps：
(1) close loach is selected
Select that physical strong, body colour is bright, the bodily form is proper, the close loach of the complete health of energetic, disease-free and injury-free, fin ray, it is male
Close loach requires body long 15-18cm, weight 15-25g, female parent's loach body long 18-25cm, weight 35-45g.
(2) cultivation of close loach
After carrying out disinfection to culture pond, female parent loach and male close loach are separately put into culture pond and carry out bath cultivation, is cultivated
Pond requirement water source is abundant, water quality is pure and fresh pollution-free, and it is 30 days to cultivate the time, and bottom of pond is laid with 20 centimetres of clay, the female parent of dispensing
The ratio of loach and male close loach is 1：4, breeding density is to put 200Kg in a suitable place to breed per acre, feeds close loach daily and cultivates feed, feeds two daily
Secondary, each feeding volume is the 8% of close loach weight.
The cultivation feed is mainly composed of the following raw materials in parts by weight：70 parts of fish meal, 35 parts of fermented bean dregs, wheat flour
30 parts, 20 parts of soybean stalk powder, 15 parts of squid liver paste, 10 parts of clam meat extract, 10 parts of calcium dihydrogen phosphate, 5 parts of choline, vitamin 5
1.5 parts of part and antioxidant.Wherein, the antioxidant is that ascorbic acid and ascorbyl palmitate press 1:1 weight ratio
It mixes.
The extracting method of the clam meat extract be take fresh clam meat to clean, drain after rub, be put into equipped with butanol solvent
Extractor in extract 60 minutes, extract 2 times, merge extracting solution, then by vacuum distillation remove butanol to get clam meat extract
Object.Wherein, the weight ratio of butanol solvent and the clam meat is 1:0.2, the temperature of extraction is 8 DEG C, the temperature of the vacuum distillation
It is 10 DEG C.
(3) injection is hastened parturition
Oxytocic drug is injected for female parent loach and male close loach, injecting method is a muscle of back shot, and oxytocic drug is suede
The human chorionic gonadtropin injection volume of Chorionic Gonadotropin and luteinizing hormone-releasing hormone analog, female parent loach is
The injection volume of 20IU/g, luteinizing hormone-releasing hormone analog are 0.1ug/g, and the dosage of male parent loach halves, and water temperature is kept
At 30 DEG C.
(4) artificial insemination
Oxytocic drug is injected after 12 hours, it is light that female parent loach abdomen is pressed to squeeze into ovum grain in vessel, then the sperm of male loach is squeezed
Go out into same vessel, be then added a concentration of 0.5% physiological saline, ovum grain and the molecular mixture of essence and is added
Physiological saline and weight ratio be 1：60, then feather or greenery is used to be gently mixed, so that ovum grain and sperm is sufficiently mixed, by
Essence obtains fertilized eggs.
(5) hatch
Fertilized eggs are gently sprinkled upon on fish nest, is transferred to during water is sufficient and the flowable hatchery of water and hatches, while toward incubating
Change and be passed through oxygen in pond, wherein the flow velocity of water is 4m/min, and the flow velocity of oxygen is 0.2m/s, to prevent fertilized eggs from quilt on fish nest
Punching is fallen, while ensureing the pure and fresh clean of water quality, the abundance of water oxygen；Water temperature is 25 DEG C, to get loach seedlings after hatching 32 hours.
(6)
(6) nursery
Loach seedlings after hatching, which continue to stay in former hatchery, to be cultivated, and the depth of water is maintained at 30-40 centimetres, every square metre of density with
1500-2000 tails are advisable；And be that soft-boiled eggs that grain size that loach seedlings feed grinding is 75 microns are yellow or fish meal, it is one day three to feed number
It is secondary；After cultivating 3-5 days, loach seedlings are gone into the raising of seedling pond.
Embodiment 3
A kind of efficient industrialized propagation method of Chinese snake head, includes the following steps：
(1) close loach is selected
Select that physical strong, body colour is bright, the bodily form is proper, the close loach of the complete health of energetic, disease-free and injury-free, fin ray, it is male
Close loach requires body long 12-16cm, weight 12-20g, female parent's loach body long 15-22cm, weight 32-40g.
(2) cultivation of close loach
After carrying out disinfection to culture pond, female parent loach and male close loach are separately put into culture pond and carry out bath cultivation, is cultivated
Pond requirement water source is abundant, water quality is pure and fresh pollution-free, and it is 28 days to cultivate the time, and bottom of pond is laid with 16 centimetres of clay, the female parent of dispensing
The ratio of loach and male close loach is 1：3, breeding density is to put 150Kg in a suitable place to breed per acre, feeds close loach daily and cultivates feed, feeds two daily
Secondary, each feeding volume is the 6% of close loach weight.
The cultivation feed is mainly composed of the following raw materials in parts by weight：60 parts of fish meal, 30 parts of fermented bean dregs, wheat flour
25 parts, 18 parts of soybean stalk powder, 12 parts of squid liver paste, 8 parts of clam meat extract, 5 parts of calcium dihydrogen phosphate, 3 parts of choline, 3 parts of vitamin
With 1 part of antioxidant.Wherein, the antioxidant is that arabo-ascorbic acid and sodium isoascorbate press 1:1 weight ratio mixing and
At.
The extracting method of the clam meat extract be take fresh clam meat to clean, drain after rub, be put into equipped with butanol solvent
Extractor in extract 50 minutes, extract 3 times, merge extracting solution, then by vacuum distillation remove butanol to get clam meat extract
Object.Wherein, the weight ratio of butanol solvent and the clam meat is 1:0.1, the temperature of extraction is 4 DEG C, the temperature of the vacuum distillation
It is 5 DEG C.
(3) injection is hastened parturition
Oxytocic drug is injected for female parent loach and male close loach, injecting method is a muscle of back shot, and oxytocic drug is suede
The human chorionic gonadtropin injection volume of Chorionic Gonadotropin and luteinizing hormone-releasing hormone analog, female parent loach is
The injection volume of 18IU/g, luteinizing hormone-releasing hormone analog are 0.2ug/g, and the dosage of male parent loach halves, and water temperature is kept
At 28 DEG C.
(4) artificial insemination
Oxytocic drug is injected after 10 hours, it is light that female parent loach abdomen is pressed to squeeze into ovum grain in vessel, then the sperm of male loach is squeezed
Go out into same vessel, a concentration of 0.45% physiological saline, ovum grain and the molecular mixture of essence is then added and is added
Physiological saline and weight ratio be 1：55, then feather or greenery is used to be gently mixed, so that ovum grain and sperm is sufficiently mixed, by
Essence obtains fertilized eggs.
(5) hatch
Fertilized eggs are gently sprinkled upon on fish nest, is transferred to during water is sufficient and the flowable hatchery of water and hatches, while toward incubating
Change and be passed through oxygen in pond, wherein the flow velocity of water is 3.5m/min, and the flow velocity of oxygen is 0.3m/s, to prevent fertilized eggs from fish nest
It is fallen by punching, while ensureing the pure and fresh clean of water quality, the abundance of water oxygen；Water temperature is 24 DEG C, to get loach after hatching 30 hours
Seedling.
(6) nursery
Loach seedlings after hatching, which continue to stay in former hatchery, to be cultivated, and the depth of water is maintained at 35-40 centimetres, every square metre of density with
1800-2000 tails are advisable；And be that soft-boiled eggs that grain size that loach seedlings feed grinding is 50 microns are yellow or fish meal, it is one day three to feed number
It is secondary；After cultivating 3-5 days, loach seedlings are gone into the raising of seedling pond.
Comparative example 1
The propagation method of the Chinese snake head of this comparative example is traditional propagation method.
Comparative example 2
The cultivation feed employed in cultivating process in the propagation method of the Chinese snake head of this comparative example except close loach is different
Outside, other are same as Example 3.
In order to verify the effect of the present invention, applicant also tries the propagation method of above-described embodiment and comparative example
Test, i.e., close loach be divided into 5 groups, 5 groups of Chinese snake heads be respectively adopted the propagation method of above-mentioned 3 embodiments and 2 comparative examples into
Row breeding, finally found that the propagation method using various embodiments of the present invention, the reproduction speed of Chinese snake head greatly improve, simultaneously also
Count the hatching survival rate of every group of Chinese snake head, specific data such as following table：
By upper table data it is found that using various embodiments of the present invention propagation method, finally obtained Chinese snake head incubates
It is melted into motility rate and is up to 97% or more, and use the hatching method of comparative example, hatching rate is not up to 90%, this shows by adopting
With the efficient industrialized propagation method of the present invention, including launch the male and female parent loach of proper proportion, breeding density appropriate, using from
The cultivation feed of system and the water flow velocity in control hatching cylinder and oxygen flow velocity etc., as fish meal, squid liver paste, the clam meat cultivated in feed carry
It takes and contains higher protein in the raw materials such as object, this promotes the gonad development of close loach, while water flow velocity appropriate and oxygen stream significantly
Speed not only ensure that the oxygen content in the pure and fresh of water quality, water is sufficient, can also prevent fertilized eggs from being fallen by punching from fish nest, finally make this
Invention substantially increases the hatching survival rate of Chinese snake head, is conducive to efficient, the large-scale breeding of Chinese snake head.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to
In the covered the scope of the claims of the present invention.
Claims (9)
1. a kind of efficient industrialized propagation method of Chinese snake head, which is characterized in that include the following steps：
(1) close loach is selected
Select that physical strong, body colour is bright, the bodily form is proper, the close loach of the complete health of energetic, disease-free and injury-free, fin ray, male parent loach
It is required that body long 10-18cm, weight 10-25g, female parent's loach body long 12-25cm, weight 30-45g；
(2) cultivation of close loach
Female parent loach and male close loach are separately put into culture pond and carry out bath cultivation, culture pond requirement water source is abundant, water quality is pure and fresh
Pollution-free, it is 25-30 days to cultivate the time, and bottom of pond is laid with 15-20 centimetres of clay, and the female parent loach of dispensing and the ratio of male close loach are
1：2-4, breeding density are to put 120-200Kg in a suitable place to breed per acre, feed close loach daily and cultivate feed, feed twice, feed every time daily
Amount is the 5%-8% of close loach weight；
The cultivation feed is mainly composed of the following raw materials in parts by weight：50-70 parts of fish meal, 25-35 parts of fermented bean dregs, wheat flour
20-30 parts of powder, 15-20 parts of soybean stalk powder, 10-15 parts of squid liver paste, 5-10 parts of clam meat extract, 2-10 parts of calcium dihydrogen phosphate,
0.5-1.5 parts of 1-5 parts of choline, 1-5 parts of vitamin and antioxidant；
(3) injection is hastened parturition
Oxytocic drug is injected for female parent loach and male close loach, injecting method is a muscle of back shot, and oxytocic drug is chorion
The human chorionic gonadtropin injection volume of promoting sexual gland hormone and luteinizing hormone-releasing hormone analog, female parent loach is 15-
The injection volume of 20IU/g, luteinizing hormone-releasing hormone analog are 0.1-0.3ug/g, and the dosage of male parent loach halves, water temperature
It is maintained at 25-30 DEG C；
(4) artificial insemination
Oxytocic drug is injected after 9-12 hours, it is light that female parent loach abdomen is pressed to squeeze into ovum grain in vessel, then the sperm of male loach is squeezed out
To in same vessel, the physiological saline of a concentration of 0.35%-0.5% is then added, is gently mixed using feather or greenery, makes ovum
Grain and sperm are sufficiently mixed, are fertilized, and obtain fertilized eggs；
(5) hatch
Fertilized eggs are gently sprinkled upon on fish nest, is transferred in water abundance and the flowable hatchery of water and hatches, while past hatchery
In be passed through oxygen, wherein the flow velocity of water is 3-4m/min, and the flow velocity of oxygen is 0.2-0.6m/s；Water temperature is 23-25 DEG C, hatching
To get loach seedlings after 28-32 hours；
(6) nursery
Loach seedlings after hatching, which continue to stay in former hatchery, to be cultivated, and the depth of water is maintained at 20-40 centimetres, every square metre of density with
1000-2000 tails are advisable；And the soft-boiled eggs Huang or fish meal of grinding are fed for loach seedlings, it is three times per day to feed number；It cultivates 3-5 days
Afterwards, loach seedlings are gone into the raising of seedling pond.
2. the efficient industrialized propagation method of Chinese snake head according to claim 1, which is characterized in that will in step (2)
Close loach also carries out disinfection to culture pond before launching culture pond.
3. the efficient industrialized propagation method of Chinese snake head according to claim 1, which is characterized in that the cultivation feed
Mainly it is composed of the following raw materials in parts by weight：60 parts of fish meal, 30 parts of fermented bean dregs, 25 parts of wheat flour, 18 parts of soybean stalk powder,
1 part of 12 parts of squid liver paste, 8 parts of clam meat extract, 5 parts of calcium dihydrogen phosphate, 3 parts of choline, 3 parts of vitamin and antioxidant.
4. the efficient industrialized propagation method of Chinese snake head according to claim 1 or 3, which is characterized in that the antioxygen
Agent is one or more of ascorbic acid, ascorbyl palmitate, arabo-ascorbic acid and sodium isoascorbate.
5. the efficient industrialized propagation method of Chinese snake head according to claim 1 or 3, which is characterized in that the clam meat
The extracting method of extract be take fresh clam meat to clean, drain after rub, be put into the extractor equipped with butanol solvent and extract 30-
It 60 minutes, extracts 2 to 4 times, merges extracting solution, then butanol is removed to get clam meat extract by vacuum distillation.
6. the efficient industrialized propagation method of Chinese snake head according to claim 5, which is characterized in that the butanol solvent
Weight ratio with the clam meat is 1:0.05-0.2.
7. the efficient industrialized propagation method of Chinese snake head according to claim 5, which is characterized in that the temperature of the extraction
Degree is 2-8 DEG C, and the temperature of the vacuum distillation is 3-10 DEG C.
8. the efficient industrialized propagation method of Chinese snake head according to claim 1, which is characterized in that step (4) is described
Ovum grain and the molecular mixture of essence and the physiological saline and weight ratio be 1：50-60.
9. the efficient industrialized propagation method of Chinese snake head according to claim 1, which is characterized in that step (6) is described
The soft-boiled eggs Huang of grinding or the grain size of fish meal are 45-75 microns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810021427.8A CN108308071A (en) | 2018-01-10 | 2018-01-10 | A kind of efficient industrialized propagation method of Chinese snake head |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810021427.8A CN108308071A (en) | 2018-01-10 | 2018-01-10 | A kind of efficient industrialized propagation method of Chinese snake head |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108308071A true CN108308071A (en) | 2018-07-24 |
Family
ID=62894215
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810021427.8A Pending CN108308071A (en) | 2018-01-10 | 2018-01-10 | A kind of efficient industrialized propagation method of Chinese snake head |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108308071A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537509A (en) * | 2019-08-14 | 2019-12-06 | 天津蕴华农业科技发展有限公司 | incubation and cultivation method of loaches |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082213A (en) * | 2014-07-15 | 2014-10-08 | 佛山市敦皇水产养殖有限公司 | Artificial propagation method for Taiwan mud fishes |
| CN105248987A (en) * | 2015-09-29 | 2016-01-20 | 池州市天健生态农业发展有限公司 | Dried tangerine peel growth promoting loach feed and preparation method thereof |
| CN105532533A (en) * | 2015-12-29 | 2016-05-04 | 太和县旭升水产养殖有限公司 | Fry cultivating method for loaches |
| CN106508739A (en) * | 2016-10-29 | 2017-03-22 | 天津蕴华农业科技发展有限公司 | Breeding method of loaches with high survival rate |
| CN107114276A (en) * | 2017-05-25 | 2017-09-01 | 河南省科学院地理研究所 | A kind of breeding method of close loach |
| CN107223605A (en) * | 2017-06-08 | 2017-10-03 | 钟选齐 | A kind of method for breeding of loach |
-
2018
- 2018-01-10 CN CN201810021427.8A patent/CN108308071A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082213A (en) * | 2014-07-15 | 2014-10-08 | 佛山市敦皇水产养殖有限公司 | Artificial propagation method for Taiwan mud fishes |
| CN105248987A (en) * | 2015-09-29 | 2016-01-20 | 池州市天健生态农业发展有限公司 | Dried tangerine peel growth promoting loach feed and preparation method thereof |
| CN105532533A (en) * | 2015-12-29 | 2016-05-04 | 太和县旭升水产养殖有限公司 | Fry cultivating method for loaches |
| CN106508739A (en) * | 2016-10-29 | 2017-03-22 | 天津蕴华农业科技发展有限公司 | Breeding method of loaches with high survival rate |
| CN107114276A (en) * | 2017-05-25 | 2017-09-01 | 河南省科学院地理研究所 | A kind of breeding method of close loach |
| CN107223605A (en) * | 2017-06-08 | 2017-10-03 | 钟选齐 | A kind of method for breeding of loach |
Non-Patent Citations (2)
| Title |
|---|
| 吴志强: "《河蟹养殖》", 30 April 2000, 中国水利水电出版社 * |
| 王复兴: "《山东土特产大全》", 30 June 1989, 济南出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110537509A (en) * | 2019-08-14 | 2019-12-06 | 天津蕴华农业科技发展有限公司 | incubation and cultivation method of loaches |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104082213A (en) | Artificial propagation method for Taiwan mud fishes | |
| CN111956783B (en) | Artificial spawning induction mixture for largehead black weever and artificial breeding method | |
| CN104872456A (en) | Universal fixed feed for grass carp and tilapia parent fish and preparation method thereof | |
| CN107853224A (en) | A kind of cultural method of cray | |
| CN105766732A (en) | Artificial reproduction method of mandarin fish | |
| CN106417122A (en) | Cultivating method for increasing high-quality freshwater shrimp seed output | |
| CN103518948A (en) | Environment-friendly compound feeds capable of improving fertility of sciaenops ocellatus parent fish | |
| CN103238563A (en) | Method for promoting female and male parent giant salamanders to synchronously mature | |
| CN105557591A (en) | Catfish ecological breeding method | |
| CN107711621A (en) | A kind of mating system of Scatophagus argus (Linnaeus) | |
| CN110612930A (en) | Artificial breeding method for rainbow trout | |
| CN107494344A (en) | A kind of artificial culturing method of advanced fry of yellow catfish | |
| CN104542478A (en) | Imitative ecological breeding and reproduction technology of andrias davidianus | |
| CN104351545A (en) | Chinese herbal medicine nutritional feed for cultivating giant salamanders | |
| CN105766731A (en) | Low-cost propagation method of bass | |
| CN105557592A (en) | Crucian ecological aquaculture method | |
| CN107439424A (en) | A kind of production of hybrid seeds of Jin Xinwu crucian carps and cultural method | |
| CN108308071A (en) | A kind of efficient industrialized propagation method of Chinese snake head | |
| CN106879545A (en) | The method for breeding of Laiwu sow under large-scale breeding management condition | |
| CN108849724A (en) | A kind of bar of Du pig and its breeding method | |
| CN106857331B (en) | Synchronous maturation control method for parent fish of rainbow trout | |
| CN108308074A (en) | A kind of hatching method of Chinese snake head | |
| CN107361021A (en) | The method that special wild boar is cultivated in wild boar and hog hybridization | |
| CN104256145A (en) | Feed for high-protein meat ducklings and preparation method thereof | |
| CN108391608A (en) | A method of improving crucian breeding quality |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180724 |
|
| RJ01 | Rejection of invention patent application after publication |