CN108251377B - 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 - Google Patents
利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 Download PDFInfo
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- CN108251377B CN108251377B CN201810041514.XA CN201810041514A CN108251377B CN 108251377 B CN108251377 B CN 108251377B CN 201810041514 A CN201810041514 A CN 201810041514A CN 108251377 B CN108251377 B CN 108251377B
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- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
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- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
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- C12N5/06—Animal cells or tissues; Human cells or tissues
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- C12N2510/00—Genetically modified cells
Abstract
Description
Component | 1× | 20× |
SYBR(2×) | 10μL | 200μL |
cDNA | 1μL | 20μL |
Nuclear-Free water | 4μL | 80μL |
15μL |
Component | 1× | 9× |
Primer F+R(10μM) | 0.8μL | 7.2μL |
Nuclear-Free water | 4.2μL | 37.8μL |
5μL |
Component | 1× | 16× |
SYBR(2×) | 10μL | 16μL |
cDNA | 1μL | 16μL |
Nuclear-Free water | 4μL | 64μL |
15μL |
Component | 1× | 5× |
Primer F+R(10μM) | 0.8μL | 4μL |
Nuclear-Free water | 4.2μL | 21μL |
5μL |
Claims (3)
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CN201810041514.XA CN108251377B (zh) | 2018-01-16 | 2018-01-16 | 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 |
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CN201810041514.XA CN108251377B (zh) | 2018-01-16 | 2018-01-16 | 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 |
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CN108251377A CN108251377A (zh) | 2018-07-06 |
CN108251377B true CN108251377B (zh) | 2021-08-27 |
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CN201810041514.XA Active CN108251377B (zh) | 2018-01-16 | 2018-01-16 | 利用携带Xist Tale抑制性转录因子R6的R6-MEF制备饲养层细胞的方法 |
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Families Citing this family (1)
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CN114134103A (zh) * | 2021-11-30 | 2022-03-04 | 北京中因科技有限公司 | 一种高效获得高活力iPSCs单克隆的方法 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004028339A2 (en) * | 2002-09-27 | 2004-04-08 | Brigham And Women's Hospital, Inc. | Treatment of patients with multiple sclerosis based on gene expression changes in central nervous system tissues |
WO2009061442A1 (en) * | 2007-11-06 | 2009-05-14 | Children's Medical Center Corporation | Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells |
CN102741394A (zh) * | 2009-10-16 | 2012-10-17 | 斯克里普斯研究所 | 多能细胞的诱导 |
CN103114075B (zh) * | 2013-01-18 | 2014-11-12 | 内蒙古大学 | 一种牛滋养层干细胞建系方法 |
CN106754729A (zh) * | 2016-12-31 | 2017-05-31 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用Xist Tale抑制性转录因子制备诱导性多能干细胞的方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101360422B (zh) * | 2005-11-23 | 2013-10-23 | 得克萨斯大学体系董事会 | 致癌Ras特异性细胞毒化合物及其使用方法 |
ES2332822T3 (es) * | 2005-11-28 | 2010-02-12 | Choongwae Pharma Corporation | Expasion sin suero de celulas en cultivo. |
CA2747161C (en) * | 2008-12-19 | 2017-07-18 | Abbott Laboratories | (hetero)aryl substituted (benzo[d]thiazol-2-ylcarbamoyl) 1,2,3,4-tetrahydroquinolins and related compounds and uses thereof for treating cancer and excess platelets |
CN109750000A (zh) * | 2017-11-06 | 2019-05-14 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用XistTale抑制性转录因子R6制备新型动物细胞系R6-MEFs方法及用途 |
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2018
- 2018-01-16 CN CN201810041514.XA patent/CN108251377B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004028339A2 (en) * | 2002-09-27 | 2004-04-08 | Brigham And Women's Hospital, Inc. | Treatment of patients with multiple sclerosis based on gene expression changes in central nervous system tissues |
WO2009061442A1 (en) * | 2007-11-06 | 2009-05-14 | Children's Medical Center Corporation | Method to produce induced pluripotent stem (ips) cells form non-embryonic human cells |
CN102741394A (zh) * | 2009-10-16 | 2012-10-17 | 斯克里普斯研究所 | 多能细胞的诱导 |
CN103114075B (zh) * | 2013-01-18 | 2014-11-12 | 内蒙古大学 | 一种牛滋养层干细胞建系方法 |
CN106754729A (zh) * | 2016-12-31 | 2017-05-31 | 内蒙古赛科星家畜种业与繁育生物技术研究院有限公司 | 利用Xist Tale抑制性转录因子制备诱导性多能干细胞的方法 |
Non-Patent Citations (3)
Title |
---|
KDM4B-mediated reduction of H3K9me3 and H3K36me3 levels improves somatic cell reprogramming into pluripotency;Jingwei Wei等;《Scientific Reports》;20170808;第7卷;第1-14页 * |
Xist Tale R-dTFs来源的小鼠iPS细胞系的建立及多能性鉴定;梁延峰;《中国优秀硕士学位论文全文数据库(电子期刊)基础科学辑》;20170215(第2期);A006-52 * |
Xist Tale R-dTFs来源的小鼠iPS细胞系的建立及多能性鉴定;梁延峰等;《中国畜牧兽医学会动物繁殖学分会第十八届学术研讨会暨中日韩第四届动物繁殖学术交流会论文集》;20160818;第148页 * |
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