CN108064698B - Tissue culture and rapid propagation method for wild medicinal material ardisia crenata - Google Patents
Tissue culture and rapid propagation method for wild medicinal material ardisia crenata Download PDFInfo
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- CN108064698B CN108064698B CN201810112129.XA CN201810112129A CN108064698B CN 108064698 B CN108064698 B CN 108064698B CN 201810112129 A CN201810112129 A CN 201810112129A CN 108064698 B CN108064698 B CN 108064698B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a tissue culture and rapid propagation method of wild medicinal material ardisia crenata, belonging to the technical field of biology. Comprises the following steps: firstly, taking strong and disease-free tender stem segments of wild ardisia crenata as explants, and inoculating the explant into a cluster bud induction culture medium for cluster bud induction culture; secondly, when the cluster buds grow to 1.5-2cm, cutting the cluster buds into single plants, and inoculating the single plants to a rooting and seedling strengthening culture medium for rooting and seedling strengthening culture; thirdly, when the rooted seedlings grow to 4-5cm, the rooted seedlings are domesticated and then transplanted; the tissue culture and rapid propagation method of the ardisia crenata adopts a tissue culture technology, and is formed by cluster bud induction culture, cutting into single plants, inoculating, rooting, seedling strengthening and culture, and finally domestication and transplanting. The method has the advantages of short seedling culture period, high survival rate, no germ, good seedling growth vigor and high yield.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a tissue culture and rapid propagation method of wild medicinal material ardisia crenata.
Background
Zhushalian lotus is a rare and rare Chinese traditional medicine, is a plant of Aristolochiaceae, is usually used as a medicine by root tuber, and depends on wild resources. Distributed in Hubei, Guangxi, Sichuan, Guizhou and Yunnan provinces. Has the effects of clearing away heat and toxic materials, regulating qi, and relieving pain. Can be used for treating carbuncle, ulcer, toxic swelling, summer-heat, dysentery, diarrhea, chest pain, abdominal pain, toothache, laryngalgia, hematemesis, and snake bite.
Zhushalian is also known: radix Aristolochiae Kaempferi. Perennial grassy vines, the whole plant is hairless. Root-shaped, irregular spindle-shaped, up to 15cm or more in length, 8cm in diameter, and 2-3 connected together, with irregular wrinkles on epidermis and yellowish or orange-yellow inner surface. The stem is slender and twisted, and has longitudinal edges and frosting. The leaf stalk is 4-15cm long; the leaf is triangular heart-shaped, and the leaf growing at the lower part of the stem is usually large, 5-14cm long, 4-11cm wide, blunt at the tip, and has a small tip and heart-shaped base; the whole edge is green on the upper part, has white faint spots and has raised lower pulse. 2-3 flowers form a short total inflorescence and even single flower axillary; the small flower stalk is slender, and the base part has one leaf-shaped bract; the flower quilt is yellow green or dark purple, the base part is spherical, the neck part is narrow and bent, the front part is enlarged and unfolded towards one side to form a tongue shape, the tongue shape is long oval, the tip is round and blunt or has a small convex tip, and 5 veins are arranged; the mouth of the pipe is provided with purple plaques and has the function of dispersing villi; the stamen sticks around the pistil, and the anther is egg-shaped; the anterior end of synanthus is 6 cleft, the base of lobe extends downward to form a wavy circular ring, the mastoid of stigma is in the shape of inverted ovate, and the ovary has 6 ridges. The capsule is oval, the base part is downward, the stem is 6-7cm long, is yellowish green, has frosting, and cracks 6 from the stem after being cooked. The seed has a triangular heart shape, is flat, has one raised surface and the other depressed surface, and has a brown appearance and is closely covered with wart-shaped bulges. The flowering period is 11 months to 4 months next year, and the fruit period is 6-10 months.
Zhu Sha Lian is resistant to yin, so it is better to shade the environment. If the illumination is too strong and the temperature is high, the seedlings are easy to die. The production area is cultivated with red purple soil. At present, only an artificial cultivation seedling transplanting method for seed propagation is available. Selecting sandy loam with inclined terrain and shady and wet soil as a seedbed, deeply ploughing and finely raking to form a high ridge. Harvesting in time after the fruits mature in the middle 6 th month, drying, removing the shells after cracking, and sowing in the last 7 th month. Sowing, covering with fine soil, and building a shed to shade. Transplanting when the seedlings are about 60 days old and 5-6 true leaves are transplanted. But the survival rate of the seed breeding and seedling raising is low, the growth vigor is uneven, the diseases are more, and the yield is low.
Disclosure of Invention
The invention aims to improve the technical difficulty of seed seedling raising and provides a tissue culture and rapid propagation method of wild medicinal material ardisia crenata.
In order to achieve the purpose, the technical scheme is as follows:
a tissue culture and rapid propagation method of wild medicinal material Aristolochia cinnabarina comprises the following steps:
1. taking strong and disease-free tender stem segments of wild ardisia crenata as explants, and inoculating the explant to a cluster bud induction culture medium for cluster bud induction culture;
2. when the cluster buds grow to 1.5-2cm, cutting the cluster buds into single plants, and inoculating the single plants to a rooting and seedling strengthening culture medium for rooting and seedling strengthening culture;
3. transplanting after the rooted seedlings grow to 4-5 cm;
the cluster bud induction culture medium is a No. 1 improved MS culture medium, and is prepared by adding 0.5mg/L, KT 0.2.2 mg/L, TDZ 0.1.1 mg/L, IBA 0.05.05 mg/L of 6-BA and 30 g/L of potato homogenate;
the rooting and seedling strengthening culture medium is a No. 2 improved MS culture medium, and IBA 0.5mg/L, GA is added 3 0.5mg/L and 30 g/L of potato homogenate;
the No. 1 improved MS culture medium refers to NH in an MS minimal medium 4 NO 3 Is 1.82g/L, KNO 3 Is 2.13g/L, KH 2 PO 4 Is 0.23g/L, CuSO 4 ·5H 2 O is 0.0005g/L, and the rest components and the concentration are unchanged.
The No. 2 improved MS culture medium refers to NH in an MS minimal medium 4 NO 3 、KNO 3 、KH 2 PO 4 、MgSO 4 ·7H 2 O、CaCl 2 The concentration is 1.5 times of the original concentration, and the other components and the concentration are unchanged.
The domestication substrate is prepared by mixing 2 parts of vermiculite, 3 parts of perlite, 4 parts of humus and 1 part of wormcast according to a volume ratio, the temperature is controlled to be 28-30 ℃ and the humidity is controlled to be 85-95% 15 days before domestication, the temperature is controlled to be 26-28 ℃ and the humidity is 75-80% after 15 days of domestication, and the field can be transplanted after 40-45 days of domestication.
The beneficial effect who adopts above-mentioned scheme does: the tissue culture and rapid propagation method of wild medicinal material Aristolochia cinnabarina adopts plant tissue culture technology, and is formed by performing cluster bud induction culture, cutting into single plants, inoculating, rooting, seedling strengthening culture, domestication and transplanting. The method has the advantages of short seedling culture period, high survival rate, no germ, good seedling growth vigor and high yield.
Detailed Description
The present invention will be further described with reference to the following examples, but the present invention is not limited to the following examples, and it is anticipated that those skilled in the art will be able to make various changes in the embodiments in combination with the prior art.
A tissue culture and rapid propagation method of wild medicinal material Ardisia crenata comprises the following steps:
1. taking strong and disease-free tender stem segments of wild ardisia crenata as explants, and inoculating the explant into a cluster bud induction culture medium for cluster bud induction culture.
2. When the cluster buds grow to 1.5-2cm, cutting the cluster buds into single plants, and inoculating the single plants to a rooting and seedling strengthening culture medium for rooting and seedling strengthening culture.
3. Transplanting after the rooted seedlings grow to 4-5 cm.
The culture medium used for inducing the cluster buds: modified MS No. 1 is used as a basic culture medium, and 0.5mg/L, KT 0.2.2 mg/L, TDZ 0.1.1 mg/L, IBA 0.05.05 mg/L of 6-BA and 30 g/L of potato homogenate are added. (NH in modified MS No. 1 4 NO 3 、KNO 3 、KH 2 PO 4 ，CuSO 4 ·5H 2 The concentrations of O were 1.82g/L, 2.13g/L, 0.23g/L, and 0.0005g/L, respectively, and the concentrations of the remaining components were unchanged).
The culture medium used for rooting and strengthening the seedlings is as follows: modified MS No. 2 as basic culture medium, adding IBA 0.5mg/L, GA 3 0.5mg/L, 30 g/L potato homogenate (NH in modified MS No. 2) 4 NO 3 、KNO 3 、KH 2 PO 4 、MgSO 4 ·7H 2 O、CaCl 2 The concentration of (b) was 1.5 times that of MS, and the concentrations of the remaining components were unchanged).
The domestication and transplanting: the domesticated substrate is formed by mixing vermiculite, perlite, humus and wormcast according to the volume ratio of 2:3:4:1, the temperature is controlled to be 28-30 ℃ and the humidity is controlled to be 85-95% 15 days before domestication, the temperature is controlled to be 26-28 ℃ and the humidity is 75-80% after domestication is carried out for 15 days, and the land can be transplanted after domestication is carried out for 40-45 days.
The tissue culture and rapid propagation method of wild medicinal material ardisia crenata adopts tissue culture technology, and is formed by cluster bud induction culture, cutting into single plants, inoculating, rooting, seedling strengthening and culture, and finally domestication and transplanting. The method has the advantages of short seedling culture period, high survival rate, no germ, good seedling growth vigor and high yield.
Claims (2)
1. A tissue culture and rapid propagation method of wild medicinal material Aristolochia cinnabarina is characterized by comprising the following steps:
(1) taking strong and disease-free tender stem segments of wild ardisia crenata as explants, and inoculating the explant into a cluster bud induction culture medium for cluster bud induction culture;
(2) when the cluster buds grow to 1.5-2cm, cutting the cluster buds into single plants, and inoculating the single plants into a rooting and seedling strengthening culture medium for rooting and seedling strengthening culture;
(3) when the rooted seedlings grow to 4-5cm, the domestication can be carried out and then the seedlings are transplanted;
the cluster bud induction culture medium is a No. 1 improved MS culture medium, and is prepared by adding 0.5mg/L, KT 0.2.2 mg/L, TDZ 0.1.1 mg/L, IBA 0.05.05 mg/L of 6-BA and 30 g/L of potato homogenate;
the rooting and seedling strengthening culture medium is a No. 2 improved MS culture medium, and IBA 0.5mg/L, GA is added 3 0.5mg/L and 30 g/L of potato homogenate;
the No. 1 improved MS culture medium refers to NH in an MS minimal medium 4 NO 3 Is 1.82g/L, KNO 3 Is 2.13g/L, KH 2 PO 4 Is 0.23g/L, CuSO 4 ·5H 2 O is 0.0005g/L, and the rest components and the concentration are unchanged;
the No. 2 improved MS culture medium refers to NH in an MS minimal medium 4 NO 3 、KNO 3 、KH 2 PO 4 、MgSO 4 ·7H 2 O、CaCl 2 The concentration of (A) is 1.5 times of the original concentration, and the other components and the concentration are unchanged.
2. The tissue culture and rapid propagation method of wild medicinal material Aristolochia cinnabarina according to claim 1, which is characterized in that: the domestication substrate in the step (3) is prepared by mixing 2 parts of vermiculite, 3 parts of perlite, 4 parts of humus and 1 part of wormcast according to a volume ratio, the temperature is controlled to be 28-30 ℃ and the humidity is controlled to be 85-95% 15 days before domestication, the temperature is controlled to be 26-28 ℃ and the humidity is 75-80% after domestication for 15 days, and the field can be transplanted after domestication for 40-45 days.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109247237A (en) * | 2018-11-22 | 2019-01-22 | 林登淞 | A kind of construction method of river Ciliatenerve Knotweed Root regenerating system |
| CN112167060B (en) * | 2020-09-30 | 2022-12-13 | 云南中医药大学 | Artificial efficient propagation method for dorsifleys |
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