CN108051417A - A kind of fluorescent inspection method of edible areca-nut brine - Google Patents
A kind of fluorescent inspection method of edible areca-nut brine Download PDFInfo
- Publication number
- CN108051417A CN108051417A CN201711346571.0A CN201711346571A CN108051417A CN 108051417 A CN108051417 A CN 108051417A CN 201711346571 A CN201711346571 A CN 201711346571A CN 108051417 A CN108051417 A CN 108051417A
- Authority
- CN
- China
- Prior art keywords
- bittern
- fluorescence
- serial
- edible areca
- inspection method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention discloses a kind of fluorescent inspection methods of edible areca-nut brine.The present invention is directed to the deficiency of the current edible areca-nut brine method of inspection, according to the fluorescent characteristics of bittern, using the correlation of fluorescence and Brine Quality and flavor, establishes a kind of fluorescent inspection method of bittern.Method of inspection high sensitivity, the result of the present invention is objective and accurate, easy to operate, has important application value the detection and analysis of edible areca-nut brine and guidance system are standby etc..
Description
Technical field
The present invention relates to edible areca-nuts to test and analyze technical field, specifically, is related to a kind of the glimmering of edible areca-nut brine
Optical check method.
Background technology
At present, Hunan Province's large size betel nut processing enterprise is up to more than 30 families, and more than 20 ten thousand tons of annual output, annual value of production is more than 10,000,000,000
Member.Cradle of the Xiang Tan as edible areca-nut, betel nut secondary industry have become the industry tap in Hunan.It is fast with betel nut industry
Speed development, betel nut secondary industry has become the pillar industry in Xiang Tan, meanwhile, also greatly advance domestic betel nut planting industry and
The industrialized development of betel nut.
Edible areca-nut production technology is complex, including choosing, clean, dry, send out son, dry, upper table is fragrant, cuts sub, point
It bittern and dries, midpoint bittern is the main characteristics of Hunan areca nut food.Bittern is typically with calcium hydroxide, maltose
After being reacted under suitable conditions for raw material with water, uniformly it is mixed with using the additive for subsequently adding various ratios
's.Reaction early period of bittern is the complicated and violent exothermic reaction that maltose occurs under high temperature and alkaline condition, and formation has
The caramelization product of hyperfluorescence.Fluorescence parameter（Fluorescence intensity and launch wavelength etc.）Stablize, the quality and flavor of bittern are also stablized.
Currently, there is edible areca-nut brine in each betel nut enterprise respective quality and flavor requirement, but to quality and flavor master
To judge by rule of thumb with sense organ, judging result varies with each individual, and subjectivity is strong, without the objective method of inspection.Fluorescence analysis
Because it has many advantages, such as highly sensitive, non-destructive and can realize real-time in-situ detection due to receives significant attention.Therefore, visitor is established
The edible areca-nut brine fluorescent inspection method of sight has important theory and realistic meaning.
The content of the invention
For the deficiency of the current edible areca-nut brine method of inspection, the present invention provides a kind of high sensitivity, the objective standard of result
Really, the fluorescent inspection method of edible areca-nut brine easy to operate, according to the fluorescent characteristics of bittern, utilizes fluorescence and Brine Quality
With the correlation of flavor, the fluorescent inspection method of bittern a kind of is established.
The purpose of the present invention is to achieve the following：
A kind of fluorescent inspection method of edible areca-nut brine, specifically includes following steps：
（1）The preparation of bittern test fluid：Serial 1.000 g of bittern samples is accurately weighed respectively, adds in appropriate distillation water dissolution,
It then moves into 100 mL volumetric flasks, is settled to 100 mL with distilled water, then series mass concentration is obtained through high speed centrifugation（I.e.
Mass fraction）For 1% bittern clear liquid, the bittern clear liquid for drawing 10 mL 1% is added in 100 mL volumetric flasks, adds 10 mL
The phosphate buffer solution of 0.2 mol/L（pH=7.4）, distilled water is added to be settled to scale, up to series mass concentration after shaking up
For 0.1% bittern test fluid；
（2）The measure of bittern fluorescence spectrum：It is 1 cm that the bittern test fluid that 3.0 mL mass concentrations are 0.1% is taken, which to add in thickness,
4 mL cuvettes in, measure the fluorescence spectrum of serial bittern respectively, fluorescence parameter is arranged to λex=350 nm, scanning wavelength model
Enclose 365-650 nm, slit width：Ex=5, Em=5；
（3）Fluorescence intensity and the launch wavelength analysis of bittern：According to fluorescence spectrum, take the fluorescence at maximum emission wavelength strong respectively
Angle value obtains the corresponding fluorescence intensity figure of serial bittern, the launch wavelength at maximum fluorescence intensity is taken to obtain serial bittern respectively
Corresponding maximum emission wavelength figure.
Further, the step（1）、（2）With（3）In serial bittern include the halogen for preparing at a temperature of differential responses
Bittern prepared by bittern, different feed way prepared by bittern prepared by water, differential responses time, different material proportioning and same
Under the conditions of different batches bittern.
Application of the fluorescent inspection method of above-mentioned edible areca-nut brine in edible areca-nut brine detection and analysis.
The beneficial effects of the present invention are：
The present invention, using the correlation of fluorescence and Brine Quality and flavor, establishes a kind of bittern according to the fluorescent characteristics of bittern
Fluorescent inspection method.This method high sensitivity, result are objective and accurate, easy to operate.Detection and system in edible areca-nut brine
It is standby etc. that there is important application value.
Description of the drawings
Fig. 1 is the fluorescence spectrum of the bittern prepared at a temperature of differential responses：The mass concentration of sample is 0.1%, fluorescence parameter
It is arranged to λex=350 nm, scanning wavelength scope 365-650 nm, slit width：Ex=5, Em=5.
Fig. 2 is that reaction temperature influences relational graph to the fluorescence intensity of bittern：The mass concentration of sample is 0.1%, fluorescence parameter
It is arranged to λex=350 nm, λem=440 nm, slit width：Ex=5, Em=5.
Fig. 3 is that reaction temperature influences relational graph to the maximum emission wavelength of bittern：The mass concentration of sample is 0.1%.Fluorescence
Parameter is arranged to λex=350 nm, slit width：Ex=5, Em=5.
Fig. 4 is the fluorescence spectra of the bittern of differential responses time：The mass concentration of sample is 0.1%, and fluorescence parameter is set
For λex=350 nm, slit width：Ex=5, Em=5.
Fig. 5 is the differential responses time to influence relational graph to the fluorescence intensity of bittern：The mass concentration of sample is 0.1%, fluorescence
Parameter is arranged to λex=350 nm, λem=440 nm, slit width：Ex=5, Em=5.
Fig. 6 is the differential responses time to influence relational graph to the maximum emission wavelength of bittern：The mass concentration of sample is 0.1%,
Fluorescence parameter is arranged to λex=350 nm, slit width：Ex=5, Em=5.
Fig. 7 attaches most importance to the maximum emission wavelength figure of the former halogen for replicating standby different batches：The mass concentration of sample is 0.1%.It is glimmering
Optical parameter is arranged to λex=350 nm, slit width：Ex=5, Em=5.
Fig. 8 is the fluorescence intensity figure of the bittern of the different batches prepared under the same terms（Fig. 8 A）With maximum emission wavelength figure
（Fig. 8 B）：The mass concentration of sample is 0.1%, and fluorescence parameter is arranged to λex=350 nm, slit width：Ex=5, Em=5.
Specific embodiment
Below in conjunction with the accompanying drawings and specific embodiment is described in further detail the present invention.
Embodiment 1
A kind of fluorescent inspection method of edible areca-nut brine, specifically includes following steps：
（1）The preparation of bittern test fluid：1.000 g of bittern samples prepared at a temperature of differential responses is accurately weighed respectively, is added in
Appropriate distillation water dissolution, then moves into 100 mL volumetric flasks, is settled to 100 mL with distilled water, then is obtained through high speed centrifugation
Serial 1% bittern clear liquid.The bittern clear liquid for drawing 10 mL 1% is added in 100 mL volumetric flasks, adds 10 mL, 0.2 mol/L
Phosphate buffer solution（pH=7.4）, distilled water is added to be settled to scale, is shaken up rear up to preparing at a temperature of differential responses
0.1% bittern test fluid；
（2）The measure of bittern fluorescence spectrum：The bittern test fluid of 3.0 mL 0.1% is taken to add in the 4 mL colorimetrics that thickness is 1 cm
In ware, the fluorescence spectrum of the bittern prepared at a temperature of differential responses is measured respectively.（Fig. 1）.Fluorescence parameter is arranged to λex=350
Nm, scanning wavelength scope 365-650 nm, slit width：Ex=5, Em=5.As shown in Figure 1, the bittern of differential responses temperature is glimmering
Light spectrum has very big difference.
（3）Fluorescence intensity and the launch wavelength analysis of bittern：According to fluorescence spectrum, take respectively glimmering at maximum emission wavelength
Light intensity value, obtain reaction temperature influences relational graph to the fluorescence intensity of bittern（Fig. 2）, the hair at maximum fluorescence intensity is taken respectively
Ejected wave is grown to obtain maximum emission wavelength influence relational graph of the reaction temperature on bittern（Fig. 3）.From Fig. 2 and Fig. 3, reaction temperature
Fluorescence intensity and maximum emission wavelength to bittern have a significant effect, and on the whole, temperature rise, fluorescence intensity and maximum are sent out
Ejected wave length is consequently increased, and fluorescence intensity and fluorescence emission wavelengths can be as the test ratings of bittern.
Embodiment 2
A kind of fluorescent inspection method of edible areca-nut brine, specifically includes following steps：
（1）The preparation of bittern test fluid：1.000 g of bittern samples of differential responses time preparation is accurately weighed respectively, is added in suitable
Amount distillation water dissolution, then moves into 100 mL volumetric flasks, 100 mL is settled to distilled water, then through high speed centrifugation system
1% bittern clear liquid of row, the bittern clear liquid for drawing 10 mL 1% are added in 100 mL volumetric flasks, add 10 mL, 0.2 mol/L's
Phosphate buffer solution（pH=7.4）, distilled water is added to be settled to scale, is shaken up rear up to the 0.1% of the preparation of differential responses time
Bittern test fluid；
（2）The measure of bittern fluorescence spectrum：The bittern test fluid of 3.0 mL 0.1% is taken to add in the 4 mL colorimetrics that thickness is 1 cm
In ware, fluorescence spectrum is measured respectively, obtains the fluorescence spectrum of the bittern of differential responses time preparation（Fig. 4）.Fluorescence parameter is set
For λex=350 nm, scanning wavelength scope 365-650 nm, slit width：Ex=5, Em=5.As shown in Figure 4, the differential responses time
The fluorescence spectrum of bittern have larger difference.
（3）Fluorescence intensity and the launch wavelength analysis of bittern：According to fluorescence spectrum, take respectively glimmering at maximum emission wavelength
Light intensity value obtains fluorescence intensity influence relational graph of the differential responses time on bittern（Fig. 5）, take respectively at maximum fluorescence intensity
Launch wavelength obtain the differential responses time relational graph influenced on the maximum emission wavelength of bittern（Fig. 6）.From Fig. 5 and Fig. 6,
Also there is larger impact in reaction time to the fluorescence intensity and maximum emission wavelength of bittern, on the whole, in certain time scope
Interior, the reaction time is longer, and fluorescence intensity and maximum emission wavelength are consequently increased, and further illustrates fluorescence intensity and fluorescent emission
Wavelength can be as the test rating of bittern.
Embodiment 3
A kind of fluorescent inspection method of edible areca-nut brine, specifically includes following steps：
（1）The preparation of bittern test fluid：The bittern samples 1.000 of the different batches prepared under the same terms are accurately weighed respectively
G adds in appropriate distillation water dissolution, then moves into 100 mL volumetric flasks, and 100 mL are settled to distilled water, then through high speed from
Gains in depth of comprehension are to serial 1% bittern clear liquid.The bittern clear liquid for drawing 10 mL 1% is added in 100 mL volumetric flasks, adds 10 mL 0.2
The phosphate buffer solution of mol/L（pH=7.4）, distilled water is added to be settled to scale, shakes up what is prepared under rear similarity condition to obtain the final product
The bittern test fluid of different batches 0.1%；
（2）The measure of bittern fluorescence spectrum：The bittern test fluid of 3.0 mL 0.1% is taken to add in the 4 mL colorimetrics that thickness is 1 cm
In ware, the fluorescence spectrum of serial bittern is measured respectively, obtains the fluorescence spectrum of the bittern of the different batches prepared under the same terms
Figure（Fig. 7）.Fluorescence parameter is arranged to λex=350 nm, scanning wavelength scope 365-650 nm, slit width：Ex=5, Em=5.By
Fig. 7 understands that the fluorescence spectrum of the bittern of the different batches prepared under the same terms is very close.
（3）Fluorescence intensity and the launch wavelength analysis of bittern：According to fluorescence spectrum, take respectively glimmering at maximum emission wavelength
Light intensity value obtains the fluorescence intensity figure of the bittern of the different batches prepared under the same terms（Fig. 8 A）, maximum fluorescence is taken respectively
Launch wavelength at intensity obtains the maximum emission wavelength figure of the bittern of the different batches prepared under the same terms（Fig. 8 B）.By scheming
8A and Fig. 8 B understand that the fluorescence intensity and maximum emission wavelength of the bittern of the different batches prepared under the same terms are essentially identical,
Fluorescence intensity and fluorescence emission wavelengths, which has been further illustrated, in this can be used as bittern test rating.
It is very reliable that above example shows that fluorescent inspection method using the present invention is detected bittern.
Above-described embodiment is only used for illustrating the present invention rather than limiting the invention, in the spirit of the present invention
In scope of the claims, to any modifications and changes made by the present invention, protection scope of the present invention is both fallen within.
Claims (3)
1. a kind of fluorescent inspection method of edible areca-nut brine, which is characterized in that include the following steps：
（1）The preparation of bittern test fluid：Serial 1.000 g of bittern samples is accurately weighed respectively, adds in appropriate distillation water dissolution,
It then moves into 100 mL volumetric flasks, is settled to 100 mL with distilled water, then series mass concentration is obtained as 1% through high speed centrifugation
Bittern clear liquid；The bittern clear liquid for drawing 10 mL 1% is added in 100 mL volumetric flasks, adds the phosphorus of 10 mL, 0.2 mol/L
Hydrochlorate buffer solution, adds distilled water to be settled to scale, shakes up rear up to the bittern test fluid that series mass concentration is 0.1%；
（2）The measure of bittern fluorescence spectrum：It is 1 cm that the bittern test fluid that 3.0 mL mass concentrations are 0.1% is taken, which to add in thickness,
In 4 mL cuvettes, the fluorescence spectrum of serial bittern is measured respectively, and fluorescence parameter is arranged to λex=350 nm, scanning wavelength scope
365-650 nm, slit width：Ex=5, Em=5；
（3）Fluorescence intensity and the launch wavelength analysis of bittern：According to fluorescence spectrum, take the fluorescence at maximum emission wavelength strong respectively
Angle value obtains the corresponding fluorescence intensity figure of serial bittern, the launch wavelength at maximum fluorescence intensity is taken to obtain serial bittern respectively
Corresponding maximum emission wavelength figure.
2. the fluorescent inspection method of edible areca-nut brine according to claim 1, it is characterised in that：The step（1）、
（2）With（3）In serial bittern include differential responses at a temperature of prepare bittern, the differential responses time prepare bittern, difference
The bittern of different batches under bittern, the bittern of different feed way preparations and identical conditions prepared by material proportion.
3. the fluorescent inspection method of the edible areca-nut brine described in claim 1 or 2 is in edible areca-nut brine detection and analysis
Using.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711346571.0A CN108051417B (en) | 2017-12-15 | 2017-12-15 | Fluorescence inspection method of edible areca-nut brine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711346571.0A CN108051417B (en) | 2017-12-15 | 2017-12-15 | Fluorescence inspection method of edible areca-nut brine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108051417A true CN108051417A (en) | 2018-05-18 |
| CN108051417B CN108051417B (en) | 2020-09-29 |
Family
ID=62133085
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711346571.0A Active CN108051417B (en) | 2017-12-15 | 2017-12-15 | Fluorescence inspection method of edible areca-nut brine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108051417B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103743842A (en) * | 2014-01-24 | 2014-04-23 | 广西大学 | Method for constructing and using Guilin brine HPLC (High Performance Liquid Chromatography) fingerprint spectrums |
| CN104483338A (en) * | 2014-12-23 | 2015-04-01 | 山东省第四地质矿产勘查院 | Method for measuring bromine in brine through filter paper method sample preparation and X-ray fluorescent spectrometry |
| CN104807777A (en) * | 2015-04-28 | 2015-07-29 | 中国食品发酵工业研究院 | Rapid detection method for areca-nut water content based on near infrared spectrum analysis technology |
-
2017
- 2017-12-15 CN CN201711346571.0A patent/CN108051417B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103743842A (en) * | 2014-01-24 | 2014-04-23 | 广西大学 | Method for constructing and using Guilin brine HPLC (High Performance Liquid Chromatography) fingerprint spectrums |
| CN104483338A (en) * | 2014-12-23 | 2015-04-01 | 山东省第四地质矿产勘查院 | Method for measuring bromine in brine through filter paper method sample preparation and X-ray fluorescent spectrometry |
| CN104807777A (en) * | 2015-04-28 | 2015-07-29 | 中国食品发酵工业研究院 | Rapid detection method for areca-nut water content based on near infrared spectrum analysis technology |
Non-Patent Citations (1)
| Title |
|---|
| 陈洪兴 等: "抗氧化剂对焦糖色素性质的影响", 《中国调味品》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108051417B (en) | 2020-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Christensen et al. | Multivariate autofluorescence of intact food systems | |
| JP6708829B1 (en) | Mixing tracking method and system for imported beef producing areas | |
| CN101477051A (en) | Raman spectrum method and reagent kit used for on-site fast detection of melamine in liquid milk | |
| SA516380091B1 (en) | Sensor for monitoring for the presence and measurement of aqueous aldehyde biocides | |
| CN104076009B (en) | A kind of method for fast measuring of biologic grain far infrared band complex refractivity index | |
| CN106442474B (en) | A kind of cement slurry three ratio measurement method based on Partial Least Squares | |
| US20110299085A1 (en) | Rapid Tissue Analysis Technique | |
| CN108195895A (en) | A kind of tea leaf nitrogen content rapid detection method based on electronic nose and spectrophotometric color measurement instrument | |
| CN109374548A (en) | A method of quickly measuring nutritional ingredient in rice using near-infrared | |
| CN109187443A (en) | Water body bacterial micro-organism based on multi-wavelength transmitted spectrum accurately identifies method | |
| CN101303304B (en) | Method for testing acrylic amide in food | |
| CN104677869A (en) | Method for detecting 17bata-estradiol by using resonance scattering spectrometry based on functional nucleic acids | |
| CN111257452B (en) | Method for detecting addition of synthetic acetic acid into Zhenjiang aromatic vinegar | |
| CN108051417A (en) | A kind of fluorescent inspection method of edible areca-nut brine | |
| CN103728269A (en) | Method for near infrared rapid detection of physical and chemical indicators in wine stock | |
| CN104350378B (en) | Method and apparatus for the performance of measure spectrum system | |
| CN105466906A (en) | Method for rapidly detecting the mixing content of pseudo-urea in milk | |
| JP5758758B2 (en) | Origin determination method of domestic wheat using trace elements | |
| JP5758244B2 (en) | Origin determination method of Australian wheat using trace elements and isotope ratio | |
| CN101576486A (en) | Method for fast measuring essence mixture ratio in cigarette | |
| CN111624181B (en) | Carbon quantum dot fluorescence sensing method for distinguishing adulteration of starch | |
| CN105067609B (en) | A kind of heat pack and the detection box as made from the heat pack and the method for carrying out concocted white spirit using box is detected and quickly testing oneself | |
| CN103959955A (en) | Method for rapidly detecting single corn seed vigor | |
| CN209640222U (en) | A kind of frying oil intelligent online detection device | |
| CN105675548A (en) | Method for determining main nutrition components in rice through using spectroscopy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |