CN108046857A - A kind of method that biological organic fertilizer is prepared using agricultural organic waste - Google Patents
A kind of method that biological organic fertilizer is prepared using agricultural organic waste Download PDFInfo
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- CN108046857A CN108046857A CN201810052350.0A CN201810052350A CN108046857A CN 108046857 A CN108046857 A CN 108046857A CN 201810052350 A CN201810052350 A CN 201810052350A CN 108046857 A CN108046857 A CN 108046857A
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
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Abstract
The invention discloses a kind of methods that biological organic fertilizer is prepared using agricultural organic waste, it is characterised in that:Include the following steps:(1)Fresh chicken manure and rice husk are pressed(0.9‑1.1):(0.8‑1)Weight ratio mixing, stack 25 30 days, every 2 days to heap body carry out turning ventilation, obtain Chicken dung;(2)Bacillus SQR9 is seeded in fluid nutrient medium ferment it is spare.(3)Take the raw material of following dry weight part:Step(1)75 79 parts of the Chicken dung of middle preparation, 57 parts of rapeseed meal, 79 parts of feather meal, 68 parts of cyanobacteria algal gel;Inoculation step after mixing(2)Biological organic fertilizer is made in the bacillus SQR9 of middle preparation, inoculation fermentation.
Description
Technical field
The present invention relates to organic fertilizer technical fields, and in particular to a kind of to prepare biological organic fertilizer using agricultural organic waste
Method.
Background technology
Mankind's industrial and agricultural production also generates substantial amounts of solid organic castoff while rapid economic development is promoted.These
Discarded object once cannot be handled timely and effectively, not only result in the wasting of resources, it is also possible to be triggered environmental pollution or even be threatened
Mankind itself.On the other hand, under intensive agricultural planting patterns, the serious threat of soil-borne disease caused by continuous single cropping
To the sustainable production of industrial crops.
Biological organic fertilizer refers to microorganisms with specific functions and mainly with plant and animal residues(Such as feces of livestock and poultry, agricultural crop straw
Deng)The one kind being combined for source and through harmless treatment, decomposed organic materials has both microbial manure and organic fertilizer efficiency
The fertilizer answered.Biological organic fertilizer nutrient is complete, can improve soil, improves and uses soil hardening caused by chemical fertilizer.Improve
Soil physical and chemical property, enhancing soil water-retaining, fertilizer conservation, the ability of fertilizer.After beneficial microbe in biological organic fertilizer enters soil
Mutual symbiosis Relationship With Proliferation is formed with Soil Microorganism, inhibits harmful bacteria and grows and be converted into beneficial bacterium, interact,
It mutually promotes, plays the synergistic effect of group, beneficial bacterium generates substantial amounts of metabolite during growth and breeding, promotes organic
The decomposition and inversion of object directly or indirectly can provide a variety of nutrition and pungent for crop, promote and regulate and control plant growth, carry
High soil porosity, penetrating exchangeability and plant survival rate increase beneficial bacterium and edaphon and population.Meanwhile in crop
The advantage profitable strain that root system is formed can inhibit harmful pathogen procreation, and the degeneration-resistant disease resistance of enhancing crop reduces continuous cropping crop
Disease index, year after year application can alleviate continuous cropping obstacle significantly.It reduces environmental pollution, is a kind of to people, animal, Environmental security, nontoxic
Environment-friendly type fertilizer.
But at present, the ordering degree of the microbiologic population of biological organic fertilizer is low in the prior art, micropopulation volume grid
Stability it is poor, influence its efficiency and quality.
The content of the invention
Using agricultural organic waste the method for biological organic fertilizer is prepared to solve existing micro- life the present invention provides a kind of
The problem of object method for preparing fertilizer method is few.
The purpose of the present invention is what is realized with following technical proposals:
A kind of method that biological organic fertilizer is prepared using agricultural organic waste, is included the following steps:
(1)Fresh chicken manure and rice husk are pressed(0.9-1.1):(0.8-1)Weight ratio mixing, stack 25-30 days, every 2 days to heap
Body carries out turning ventilation, obtains Chicken dung;
(2)Bacillus SQR9 is seeded in fluid nutrient medium ferment it is spare.
(3)Take the raw material of following dry weight part:Step(1)75-79 parts of the Chicken dung of middle preparation, 5-7 parts of rapeseed meal, feather
7-9 parts of powder, 6-8 parts of cyanobacteria algal gel, 5-7 parts of soybean slag;Inoculation step after mixing(2)The bacillus SQR9 of middle preparation,
Biological organic fertilizer is made in inoculation fermentation.
Preferably, a kind of method that biological organic fertilizer is prepared using agricultural organic waste, is included the following steps:
(1)Fresh chicken manure and rice husk are pressed 1:0.9 weight ratio mixing, stacks 28 days under 25 DEG C of environment, every 2 days to heap body into
Row turning is ventilated once, obtains Chicken dung;
(2)Bacillus SQR9 is seeded in LB fluid nutrient mediums, fermentation condition 170r/min, is cultivated under the conditions of 30 DEG C
36h;5700 r/min centrifuge 7min after fermentation, and it is spare that thalline is resuspended with sterile water.
(3)Take the raw material of following dry weight part:Step(1)77 parts of the Chicken dung of middle preparation, 8 parts of rapeseed meal, feather meal 8
Part, 7 parts of cyanobacteria algal gel, 6 parts of soybean slag;Inoculation step after mixing(2)The bacillus SQR9 of middle preparation, inoculum concentration are
7.5% volume ratio is fermented 7 days under the conditions of 25 DEG C, and twice, biological organic fertilizer is made in daily turning.
Beneficial effects of the present invention are as follows:
1st, the present invention be reasonably formulated and preparation method made from biological organic fertilizer there is apparent yield increasing effect, and biological organic
The ordering degree of the microbiologic population of fertilizer is high, and the stability of micropopulation volume grid is high, is suitble to continuous plantation and sustainable development
Exhibition.
2nd, the biological organic fertilizer in the present invention has an effect for the disease rates for reducing bacterial wilt, and effect is more during continuous plantation
Substantially.
3rd, the biological organic fertilizer in the present invention has Vc contents, titrable acidity and the soluble sugar content for improving fruit,
Therefore the biological organic fertilizer of the present invention can improve fruit quality.
Specific embodiment
Embodiment 1
A kind of method that biological organic fertilizer is prepared using agricultural organic waste, is included the following steps:
(1)Fresh chicken manure and rice husk are pressed(0.9-1.1):(0.8-1)Weight ratio mixing, stack 25-30 days, every 2 days to heap
Body carries out turning ventilation, obtains Chicken dung;
(2)Bacillus SQR9 is seeded in fluid nutrient medium ferment it is spare.
(3)Take the raw material of following dry weight part:Step(1)75-79 parts of the Chicken dung of middle preparation, 5-7 parts of rapeseed meal, feather
7-9 parts of powder, 6-8 parts of cyanobacteria algal gel, 5-7 parts of soybean slag;Inoculation step after mixing(2)The bacillus SQR9 of middle preparation,
Biological organic fertilizer is made in inoculation fermentation.
Embodiment 2
A kind of method that biological organic fertilizer is prepared using agricultural organic waste, is included the following steps:
(1)Compost:Fresh chicken manure and rice husk are pressed 1:0.9 weight ratio mixing, stacks 28 days, every 2 days right under 25 DEG C of environment
Heap body carries out turning ventilation, obtains Chicken dung;
(2)The preparation of seed liquor:Bacillus SQR9 is seeded in LB fluid nutrient mediums, fermentation condition 170r/min, 30
36h is cultivated under the conditions of DEG C;5700 r/min centrifuge 7min after fermentation, and it is spare that thalline is resuspended with sterile water.
(3)Take the raw material of following dry weight part:Step(1)77 parts of the Chicken dung of middle preparation, 8 parts of rapeseed meal, feather meal 8
Part, 7 parts of cyanobacteria algal gel, 6 parts of soybean slag;Inoculation step after mixing(2)The bacillus SQR9 of middle preparation, inoculum concentration are
7.5% volume ratio is fermented 7 days under the conditions of 25 DEG C, and twice, biological organic fertilizer is made in daily turning.
Comparative example 1
A kind of method that biological organic fertilizer is prepared using agricultural organic waste, is included the following steps:
(1)Compost:Fresh chicken manure and rice husk are pressed 1:0.9 weight ratio mixing, stacks 28 days, every 2 days right under 25 DEG C of environment
Heap body carries out turning ventilation, obtains Chicken dung;
(2)Take the raw material of following dry weight part:Step(1)77 parts of the Chicken dung of middle preparation, 8 parts of rapeseed meal, 8 parts of feather meal, indigo plant
7 parts of algae algal gel, 6 parts of soybean slag;Compost is carried out after mixing, the compost phase is 15 days.
Raw material and additive amount determine to test
1st, chicken manure and rice husk addition are than determining experiment
Heap body A:2 step of embodiment(1)In Chicken dung heap body obtained;
Heap body B:Fresh chicken manure and rice husk are pressed 1:0.2 weight ratio mixing stacks 28 days, carries out turning to heap body within every 2 days and lead to
Chicken dung heap body is made in gas.
Heap body C:Fresh chicken manure and rice husk are pressed 1:0.5 weight ratio mixing, stacks 28 days, heap body was turned in every 2 days
Heap is ventilated, and Chicken dung heap body is made.
The temperature change of heap body A, heap body B and heap body C, change of moisture content, carbon-nitrogen ratio, total phosphorus and total potassium content are surveyed
Fixed, measurement result is as shown in the table.
1 temperature variation data table of table(Unit:℃)
Initial temperature | 3rd day | 6th day | 9th day | 12nd day | 15th day | 18th day | 21st day | 24th day | Compost terminates | |
Heap body A | 30 | 67 | 66 | 68 | 69 | 67 | 63 | 62 | 56 | 55 |
Heap body B | 30 | 45 | 51 | 65 | 63 | 68 | 64 | 59 | 51 | 52 |
Heap body C | 30 | 51 | 59 | 66 | 67 | 68 | 65 | 61 | 54 | 53 |
It can be seen from the data in Table 1 that the heating rate of heap body A is faster than heap body B and heap body C, and 60 DEG C or more duration
Than heap body B and heap body C long, illustrate that the decomposed rate of heap body A is most fast.
2 change of moisture content tables of data of table(Unit:%)
Initial water content | 3rd day | 6th day | 9th day | 12nd day | 15th day | 18th day | 21st day | 24th day | Compost terminates | |
Heap body A | 68 | 67 | 65 | 64 | 60 | 48 | 39 | 30 | 23 | 22 |
Heap body B | 76 | 71 | 58 | 55 | 48 | 36 | 30 | 27 | 19 | 18 |
Heap body C | 72 | 68 | 63 | 62 | 53 | 46 | 36 | 29 | 25 | 21 |
It can be seen from the data in Table 2 that water content suppression ratio the heap body B and heap body C of heap body A are slow, this illustrates that heap body A can allow micro- life
Object preferably carries out physiological metabolism activity.
3 carbon-nitrogen ratio delta data table of table
Initial carbon-nitrogen ratio | 3rd day | 6th day | 9th day | 12nd day | 15th day | 18th day | 21st day | 24th day | Compost terminates | |
Heap body A | 17.3 | 19.5 | 20.1 | 23.8 | 17.6 | 17.3 | 17.5 | 18.1 | 18.0 | 18.1 |
Heap body B | 9.8 | 11.6 | 12.5 | 14.0 | 14.8 | 14.9 | 15.2 | 17.3 | 17.1 | 17.0 |
Heap body C | 13.4 | 14.3 | 14.8 | 15.2 | 15.9 | 16.2 | 16.5 | 16.8 | 17.2 | 17.4 |
It can be seen from the data in Table 3 that the initial carbon-nitrogen ratio of heap body A, heap body B and heap body C are 17.3,9.8 and 13.4, compost knot
The carbon-nitrogen ratio of heap body A than initially increases 0.9 during beam, and the carbon-nitrogen ratio of heap body B increases 7.2, and the carbon-nitrogen ratio of heap body C increases
4.0, this is because the carbon-nitrogen ratio of heap body B and heap body C are low, cause ammonia volatilization and cause the loss of nitrogen, and then slow down organic materials
Degradation.
4 total phosphorus of table and total potassium content delta data table(Unit:%)
It can be seen from the data in Table 4 that the total phosphorus initial content of heap body A and total potassium initial content are below heap body B and heap body C,
But the total phosphorus and total potassium content gap at the end of compost are little, illustrate that the total phosphorus content of heap body A and total potassium content are in rising trend.
Can be seen that the raw material ratio in embodiment 2 from said determination result is composting material coefficient optimum proportion.
2nd, Chicken dung and the addition of cyanobacteria algal gel are than determining experiment
T1:Chicken dung;
T2:99 parts of Chicken dung(DW), 1 part of cyanobacteria algal gel(DW);
T3:97 parts of Chicken dung(DW), 3 parts of cyanobacteria algal gel(DW);
T4:95 parts of Chicken dung(DW), 5 parts of cyanobacteria algal gel(DW);
T5:93 parts of Chicken dung(DW), 7 parts of cyanobacteria algal gel(DW);
T6:91 parts of Chicken dung(DW), 9 parts of cyanobacteria algal gel(DW);
Total nitrogen content, total carbon content, pH value, Water Soluble Organic Carbon content and water soluble organic nitrogen content are measured to above-mentioned sample,
Its measurement result is as shown in the table.Wherein T6 samples compost due to excess moisture fails, and can not measure its physicochemical property.
The physicochemical property of organic fertilizer is made in the different jujube paste additive amounts of table 5
Total nitrogen(%) | Total carbon(%) | pH | Water Soluble Organic Carbon(mg/gDW) | Water soluble organic nitrogen(mg/gDW) | |
T1 | 1.9 | 17.8 | 8.6 | 23.5 | 2.7 |
T2 | 1.9 | 18.4 | 8.1 | 26.5 | 2.8 |
T3 | 2.0 | 18.1 | 8.0 | 30.1 | 2.8 |
T4 | 2.0 | 17.4 | 7.9 | 29.5 | 3.0 |
T5 | 2.1 | 17.0 | 7.8 | 26.3 | 3.5 |
The adding proportion that can be seen that the cyanobacteria algal gel of T5 samples from the data in table 5 is optimal.
3rd, inoculum concentration determines to test
T7:77 parts of Chicken dung(DW), 8 parts of rapeseed meal(DW), 8 parts of feather meal(DW), 7 parts of cyanobacteria algal gel(DW), 6 parts of soybean slag
(DW), initial water content 50%, inoculation 2 step of embodiment(2)In seed liquor, inoculum concentration is:2.5%;
T8:77 parts of Chicken dung(DW), 8 parts of rapeseed meal(DW), 8 parts of feather meal(DW), 7 parts of cyanobacteria algal gel(DW), 6 parts of soybean slag
(DW), initial water content 50%, inoculation 2 step of embodiment(2)In seed liquor, inoculum concentration is: 5%;
T9:77 parts of Chicken dung(DW), 8 parts of rapeseed meal(DW), 8 parts of feather meal(DW), 7 parts of cyanobacteria algal gel(DW), 6 parts of soybean slag
(DW), initial water content 50%, inoculation 2 step of embodiment(2)In seed liquor, inoculum concentration is: 7.5%;
T10:77 parts of Chicken dung(DW), 8 parts of rapeseed meal(DW), 8 parts of feather meal(DW), 7 parts of cyanobacteria algal gel(DW), 6 parts of soybean slag
(DW), initial water content 50%, inoculation 2 step of embodiment(2)In seed liquor, inoculum concentration is: 10%;
T11:77 parts of Chicken dung(DW), 8 parts of rapeseed meal(DW), 8 parts of feather meal(DW), 7 parts of cyanobacteria algal gel(DW), 6 parts of soybean slag
(DW), initial water content 50%, inoculation 2 step of embodiment(2)In seed liquor, inoculum concentration is: 12.5%.
Above-mentioned sample is carried out under the conditions of 20 DEG C -45 DEG C to secondary solid to ferment 7 days, daily turning once-tetra- time, system
Obtain biological organic fertilizer.
In entire fermentation process, what the secondary solid of fermentation was fermented apparent increase occurs for bacterial strain SQR9 contents for first 3 days
Add, then drop to fermentation ends always, therefore this research is in subsequent factor Selection experiment, monitoring secondary solid fermentation the
At 3 days and the 7th day in heap body function bacterium quantity.In the result of the test of temperature factor, the quantity of function bacterium is at 20 DEG C to 45
There is no notable difference under DEG C scope, it is wider that this illustrates that bacterial strain SQR9 adapts to temperature range.
When initial inoculum is less than 7.5%, bacterial strain SQR9 contents increase with increasing for inoculum concentration, this explanation
7.5% is suitable initial inoculum.Turn pile frequency on bacterial strain SQR9 contents influence the results show that when turn pile frequency is more than every
It twice when, bacterial strain SQR9 contents decline on the 3rd day in fermentation, and it is certain that this illustrates that turning generates heap body heat content and dissolved oxygen content
Influence.Therefore in subsequent research, turn pile frequency selected as turns over throwing twice daily.
Influence experiment of the biological organic fertilizer to tomato growth and quality
1st, test method
5 processing are set altogether:
CK:Not fertilizer treatment;
A:It handles to fertilize;
B:The organic fertilizer processing prepared in comparative example 1 is applied, dose is 300kg/ mus;
C:The organic fertilizer processing prepared in embodiment 2 is applied, dose is 300kg/ mus.
The nutrients such as each processing are set, the nutrient chemical fertilizer polishing nutrient as needed for the tomato time of infertility.Each processing sets three
A repetition, each plot area are 5m2, planting density is per cell 15.Field planting starts meter production after 60 days, and counts morbidity
Rate.Incidence apparatus bacterial wilt symptom plant accounts for the percentage of total plant number to represent, with 75 days for a season, for planting the four seasons.
2nd, the measure of Tomato Quality
It chooses the tamato fruit after being colonized 75 days in the first season and carries out quality determination, testing index is Vc contents, titrable acidity
(TA), soluble sugar(TSS)Content.Wherein, Vc assays use 2,6-D titrations, and titrable acidity is dripped using standard base (SB)
Determine method, the measure of soluble sugar content uses anthrone method.
3rd, result of the test
Continuous administration biological organic fertilizer(C processing)Less fertilising(CK processing), using chemical fertilizer(A processing)And application of organic fertilizers(B
Processing)Significantly improve the yield of tomato.
Influence tables of data of 1 biological organic fertilizer of table to tomato yield(Unit:Kg/ mus)
The first season | The second season | The third quarter | The fourth season | |
CK processing | 3700 | 3300 | 900 | 950 |
A processing | 4800 | 3100 | 1100 | 1200 |
B processing | 4800 | 3600 | 2200 | 1300 |
C processing | 4900 | 4700 | 3500 | 3100 |
Can be seen that from upper table data, in the second season, C processing compared with CK processing, A processing and B processing respectively voluminous 42.4%,
51.6% and 30.6%.In the third quarter, C processing is compared with 288.9%, 218.2% and of CK processing, A processing and B processing difference fecund
59.1%.In the fourth season, C processing is compared with CK processing, A processing and B processing fecund 226.2%, 158.3% and 138.5%, C processing respectively
With apparent yield increasing effect, and sustainable developability is good.
2 biological organic fertilizer of table influences tables of data to the bacterial wilt of reduction(Unit:%)
The first season | The second season | The third quarter | The fourth season | |
CK processing | 0 | 20 | 76 | 70 |
A processing | 0 | 23 | 68 | 65 |
B processing | 0 | 18 | 51 | 67 |
C processing | 0 | 6 | 33 | 34 |
It can be seen that from upper table data, in the second season, C processing significantly reduces the incidence of bacterial wilt of tomato.In the third quarter
In, the incidence of CK processing, A processing and B processing is respectively that 76%, 68% and 51%, C are handled compared at CK processing, A processing and B
The disease rates that reason inhibits bacterial wilt are respectively 43%, 35% and 18%.In the fourth season, C processing exists than A processing and B processing
31% and 33% are reduced on bacterial wilt incidence.With the increase of continuous implantation time, bacterial wilt of tomato between each processing
Disease rates have significant difference, and C processing can the effectively native generation for passing bacterial wilt of prevention and control tomato.
To the first season, the Quality of Tomato Fruit of production is measured, and determination data is as shown in table 3.
3 biological organic fertilizer of table influences tables of data to Tomato Quality(Unit:)
Vc (mg/100g) | Titrable acidity(%) | Soluble sugar content(%) | |
CK processing | 7.35±1.51 | 0.3 | 7.8±0.3 |
A processing | 7.72±1.52 | 0.2 | 7.6±0.2 |
B processing | 7.78±1.35 | 0.3 | 7.8±0.5 |
C processing | 9.12±0.76 | 0.4 | 10.6±0.4 |
It can be seen that from upper table data, in fruit quality, C processing can improve Vc contents, titrable acidity and the solubility of fruit
Sugared content.This illustrates that C processing can improve fruit quality.
4th, conclusion (of pressure testing)
(1)Biological organic fertilizer is applied with apparent yield increasing effect, and sustainable developability is good during continuous plantation;
(2)With the increase of continuous implantation time, the disease rates of bacterial wilt of tomato have significant difference between each processing, and
Its incidence significantly raises during continuous plantation, and apply biological organic fertilizer has significant effect to prevention and control tomato soil biography bacterial wilt
Fruit;
(3)Vc contents, titrable acidity and the soluble sugar content for applying the tomato of biological organic fertilizer are above not applying biological organic
The tomato of fertilizer, this explanation, which applies biological organic fertilizer, can improve fruit quality.
Claims (2)
- A kind of 1. method that biological organic fertilizer is prepared using agricultural organic waste, it is characterised in that:Include the following steps:(1)Fresh chicken manure and rice husk are pressed(0.9-1.1):(0.8-1)Weight ratio mixing, stack 25-30 days, every 2 days to heap Body carries out turning ventilation, obtains Chicken dung;(2)Bacillus SQR9 is seeded in fluid nutrient medium ferment it is spare;(3)Take the raw material of following dry weight part:Step(1)75-79 parts of the Chicken dung of middle preparation, 5-7 parts of rapeseed meal, feather meal 7- 9 parts, 6-8 parts of cyanobacteria algal gel, 5-7 parts of soybean slag;Inoculation step after mixing(2)The bacillus SQR9 of middle preparation, inoculation Biological organic fertilizer is made in fermentation.
- 2. a kind of method that biological organic fertilizer is prepared using agricultural organic waste according to claim 1, feature are existed In:Include the following steps:(1)Fresh chicken manure and rice husk are pressed 1:0.9 weight ratio mixing, stacks 28 days under 25 DEG C of environment, every 2 days to heap body into Row turning is ventilated once, obtains Chicken dung;(2)Bacillus SQR9 is seeded in LB fluid nutrient mediums, fermentation condition 170r/min, is cultivated under the conditions of 30 DEG C 36h;5700 r/min centrifuge 7min after fermentation, and it is spare that thalline is resuspended with sterile water;(3)Take the raw material of following dry weight part:Step(1)77 parts of the Chicken dung of middle preparation, 8 parts of rapeseed meal, 8 parts of feather meal, indigo plant 7 parts of algae algal gel, 6 parts of soybean slag;Inoculation step after mixing(2)The bacillus SQR9 of middle preparation, inoculum concentration are 7.5% body Product ratio, ferments 7 days under the conditions of 25 DEG C, and twice, biological organic fertilizer is made in daily turning.
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CN110981633A (en) * | 2019-12-20 | 2020-04-10 | 鹏鹞环保股份有限公司 | Development method of functional composite microbial fertilizer special for edible olive trees |
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CN110981633A (en) * | 2019-12-20 | 2020-04-10 | 鹏鹞环保股份有限公司 | Development method of functional composite microbial fertilizer special for edible olive trees |
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