CN108040530A - The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method - Google Patents
The wild Paris polyphylla seed dormancy in Dabie Mountain breaks method Download PDFInfo
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- CN108040530A CN108040530A CN201711312282.9A CN201711312282A CN108040530A CN 108040530 A CN108040530 A CN 108040530A CN 201711312282 A CN201711312282 A CN 201711312282A CN 108040530 A CN108040530 A CN 108040530A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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Abstract
The invention discloses a kind of wild Paris polyphylla seed dormancies in Dabie Mountain to break method, it is related to Chinese medicine cultivation field, the wild Paris polyphylla seed dormancy in the Dabie Mountain breaks method using kind of skin processing and sterilizing and seed dormancy is broken step by step, its stratification duration is short, it shorten to handle for 6 months by processing in original 18 months, this method solves Chinese medicine farmer and plants Paris polyphylla time cost.Germination rate is high, and after this method processing, Paris polyphylla seed germination rate is up to more than 95%.Originally processing mode seed germination rate is very low, below 50%.
Description
Technical field
The present invention relates to Chinese medicine cultivation field, it is more particularly related to which a kind of wild Paris polyphylla seed in Dabie Mountain is stopped
Method is broken in dormancy.
Background technology
Paris polyphylla dormancy degree is high, and it is slow to sprout rate, and germination rate is low, it is necessary to could complete within 18 months dormancy period, of the invention
Crucial purpose be shorten dormancy time.The main reason for Paris polyphylla seed dormancy is that seed has germination inhibitor, embryo development
Not exclusively and physiology after-ripening, with reference to forefathers the study found that after high temperature Stratificated treatment seed i.e. with completion morphology after-ripening, but
Seed must still carry out a cool stratification, by physiology after-ripening, could promote the growth of plumular axis, be indicated above the sprouting of Paris polyphylla seed
Early period is limited by morphology after-ripening, and seed is sprouted and limited by physiology after-ripening after morphology maturation.
The content of the invention
Problem to be solved by this invention is to provide a kind of wild Paris polyphylla seed dormancy in Dabie Mountain and breaks method, according to current
Paris polyphylla Mechanism of Dormancy and the method used in the feature present invention can quickly break the wild Paris polyphylla seed dormancy phase in Dabie Mountain.
To achieve these goals, the technical solution taken of the present invention is：
The wild Paris polyphylla seed dormancy in the Dabie Mountain is broken method and is included the following steps：
(1) skin processing and sterilizing are planted：Exosper must remove, and can first be impregnated for 24 hours with 25 DEG C of warm water, then fresh seeds and
Wet sand mixes, and rubs red exosper with the hands, and Seed sterilization processing is first impregnated 1~2 minute with 75% ethyl alcohol, then with 0.2% mercuric chloride
Sterilizing 15 minutes, lamination add in 50% carbendazim and keep the inside long-term sterile in the process, and sand is gone out with 100 DEG C or more high-temperature steams
Bacterium；
(2) seed dormancy breaking steps：Paris polyphylla seed afterripening is totally divided at warm temperature-low temperature-warm warm three-level alternating temperature stratification
Reason, lamination was carried out with 180 days, is warmed up warm 60 days constant temperature processing times, is made of two stages, because 40 days be a material time section
Point, therefore it is divided into form after-ripening early period and later stage, by there is " secondary dormancy " characteristic because of Paris polyphylla seed, it is necessary to have at cryogenic thermostat
Reason, the low-temperature treatment time is 60 days, is then transferred to warm warm intermittent warming, it is necessary to be 20 DEG C and 15 DEG C intermittent warming 40 days round the clock,
Into final stage, warming up the vernalization of temperature constant temperature needs 20 days.
Five stages in above-mentioned seed dormancy breaking steps are respectively：
(1) first stage is form after-ripening early period (0-40 days) (humiture promotes seed morphology after-ripening)：First 40 days
Warm layer product is to reduce endogenous aba content, and causing embryo development, and during this, it is invalid that first 40 days, which apply exogenous GA 3,
, but can have antagonism to ABA, while complete that there is facilitation to the after-ripening of embryo form, it can apply on a small quantity, contract
Short form ripening time, exogenous GA 3 concentration are 10ppm, this stage needs to be that warm layer accumulates 20 DEG C of degrees Celsius of processing needs
Time is 60 days；
(2) second stage is form after-ripening later stage (40-60 days) (humiture promotes seed morphology after-ripening)：Take out warm temperature
Then seed of the lamination after 40 days carries out Seed sterilization processing, this is that Paris polyphylla seed switchs to abolish from dormant state within 40-60 days
One critical period of dormant state, in form After-ripening, strictly by 20 DEG C of completion morphology after-ripening relative humidity requirements
It is that 60-90% is kept with humidifier, while 3-5 days will stir in time, help oxygen supply and divulge information, funginert growth,
But during 40-60 days, apply exogenous GA 3, GA3 concentration for the treatment of is unsuitable excessively high, it should maintain a reduced levels, concentration
For 100ppm, Paris polyphylla seed after processing in 60 days is accumulated by warm layer, develop from subsphaeroidalization for cylindrical embryo, lower embryo by embryo
Axis expands overstriking, gradually forms rhizome, completion morphology after-ripening；
(3) phase III is physiological after-ripening stage (60-120 days) (hormone control completes physiological after-ripening)：Cryosphere
Product induction be again need in this stage of key point be the processing of 5 DEG C degrees Celsius of cool stratification, it is necessary to time be 60 days, apply
Exogenous GA 3, concentration for the treatment of 200mg/l impregnate for 24 hours, further take out to admix in 1: 5 ratio after drying and treated through high-temperature sterilization
Wet sand in, be put into growth cabinet and carry out stratification, the condition of growth cabinet is set as：18 DEG C of temperature, humidity 70%,
Apply external source AC (activated carbon) simultaneously to be adsorbed, AC and wet sand volume ratio are 500: 1；
(4) fourth stage is the alternately alternating temperature stratification processing (120-160 days) of warm temperature：With 20 DEG C of daytime, night is at 15 DEG C
Reason, it is necessary to time be 40 days or so.And applying exogenous GA 3, concentration for the treatment of 200mg/l is impregnated for 24 hours, further taken out after drying
It is admixed in 1: 5 ratio and carries out stratification, growth cabinet through in the processed wet sand of high-temperature sterilization, being put into growth cabinet
Condition be set as：18 DEG C of temperature, humidity 70%；
(5) the 5th stages were vernalization stage (120-160 days)：This stage needs to be warm warm 20 DEG C degrees Celsius of constant temperature lamination
, it is necessary to which the time is 20 days or so, wet sand processing is same as above for processing, and Inhibiting germination substance exists unrelated with physiological after-ripening but easy
Endogenous Inhibiting germination substance is discharged, it is necessary to apply external source AC (activated carbon), AC and wet sand volume ratio are 500: 1, can improve and sprout
Hair rate.
Advantageous effect：The stratification duration that the wild Paris polyphylla seed dormancy in the Dabie Mountain breaks method is short, by original 18
Moon processing shorten to handle for 6 months, and this method solves Chinese medicine farmer and plants Paris polyphylla time cost.Germination rate is high, this method
After processing, Paris polyphylla seed germination rate is up to more than 95%.Originally processing mode seed germination rate is very low, below 50%.
Specific embodiment
Break the preferred embodiment of method the following detailed description of the wild Paris polyphylla seed dormancy in Dabie Mountain of the present invention.
The wild Paris polyphylla seed dormancy in the Dabie Mountain is broken method and is included the following steps：
(1) skin processing and sterilizing are planted：Exosper must remove, and can first be impregnated for 24 hours with 25 DEG C of warm water, then fresh seeds and
Wet sand mixes, and rubs red exosper with the hands, and Seed sterilization processing is first impregnated 1~2 minute with 75% ethyl alcohol, then with 0.2% mercuric chloride
Sterilizing 15 minutes, lamination add in 50% carbendazim and keep the inside long-term sterile in the process, and sand is gone out with 100 DEG C or more high-temperature steams
Bacterium；There is experiment to show seed there is no water suction obstacle, outer seed does not have Inhibiting germination substance, but prevents Inhibiting germination substance row
Go out, it is necessary to carry out outer seed processing.
(2) seed dormancy breaking steps：Paris polyphylla seed afterripening is totally divided at warm temperature-low temperature-warm warm three-level alternating temperature stratification
Reason, lamination was carried out with 180 days, is warmed up warm 60 days constant temperature processing times, is made of two stages, because 40 days be a material time section
Point, therefore it is divided into form after-ripening early period and later stage, by there is " secondary dormancy " characteristic because of Paris polyphylla seed, it is necessary to have at cryogenic thermostat
Reason, the low-temperature treatment time is 60 days, is then transferred to warm warm intermittent warming, it is necessary to be 20 DEG C and 15 DEG C intermittent warming 40 days round the clock,
Into final stage, warming up the vernalization of temperature constant temperature needs 20 days.
Five stages in above-mentioned seed dormancy breaking steps are respectively：
(1) first stage is form after-ripening early period (0-40 days) (humiture promotes seed morphology after-ripening)：First 40 days
Warm layer product is to reduce endogenous aba content, and causing embryo development, and during this, it is invalid that first 40 days, which apply exogenous GA 3,
, but can have antagonism to ABA, while complete that there is facilitation to the after-ripening of embryo form, it can apply on a small quantity, contract
Short form ripening time, exogenous GA 3 concentration are 10ppm, this stage needs to be that warm layer accumulates 20 DEG C of degrees Celsius of processing needs
Time is 60 days；
(2) second stage is form after-ripening later stage (40-60 days) (humiture promotes seed morphology after-ripening)：Take out warm temperature
Then seed of the lamination after 40 days carries out Seed sterilization processing, this is that Paris polyphylla seed switchs to abolish from dormant state within 40-60 days
One critical period of dormant state, in form After-ripening, strictly by 20 DEG C of completion morphology after-ripening relative humidity requirements
It is that 60-90% is kept with humidifier, while 3-5 days will stir in time, help oxygen supply and divulge information, funginert growth,
But during 40-60 days, apply exogenous GA 3, GA3 concentration for the treatment of is unsuitable excessively high, it should maintain a reduced levels, concentration
For 100ppm, Paris polyphylla seed after processing in 60 days is accumulated by warm layer, develop from subsphaeroidalization for cylindrical embryo, lower embryo by embryo
Axis expands overstriking, gradually forms rhizome, completion morphology after-ripening；This period removal exosper may remove Inhibiting germination substance,
It helps and carries out form after-ripening with seed, thus germination inhibitor may be unrelated with physiology after-ripening, the influence to seed dormancy
Mainly in the later stage of morphology latter stage of ripening.
(3) phase III is physiological after-ripening stage (60-120 days) (hormone control completes physiological after-ripening)：Cryosphere
Product induction be again need in this stage of key point be the processing of 5 DEG C degrees Celsius of cool stratification, it is necessary to time be 60 days, apply
Exogenous GA 3, concentration for the treatment of 200mg/l impregnate for 24 hours, further take out to admix in 1: 5 ratio after drying and treated through high-temperature sterilization
Wet sand in, be put into growth cabinet and carry out stratification, the condition of growth cabinet is set as：18 DEG C of temperature, humidity 70%,
Apply external source AC (activated carbon) simultaneously to be adsorbed, AC and wet sand volume ratio are 500: 1；GA3 contributes to the physiology after-ripening of seed,
The hormone control in this stage is to complete physiological after-ripening, and the presence of germination inhibitor may inhibit embryo development, and with life
Rationality After-ripening is unrelated.But it is easily drained endogenous Inhibiting germination substance and is adsorbed, it is necessary to apply external source AC (activated carbon).
(4) fourth stage is the alternately alternating temperature stratification processing (120-160 days) of warm temperature：With 20 DEG C of daytime, night is at 15 DEG C
Reason, it is necessary to time be 40 days or so.And applying exogenous GA 3, concentration for the treatment of 200mg/l is impregnated for 24 hours, further taken out after drying
It is admixed in 1: 5 ratio and carries out stratification, growth cabinet through in the processed wet sand of high-temperature sterilization, being put into growth cabinet
Condition be set as：18 DEG C of temperature, humidity 70%；
(5) the 5th stages were vernalization stage (120-160 days)：This stage needs to be warm warm 20 DEG C degrees Celsius of constant temperature lamination
, it is necessary to which the time is 20 days or so, wet sand processing is same as above for processing, and Inhibiting germination substance exists unrelated with physiological after-ripening but easy
Endogenous Inhibiting germination substance is discharged, it is necessary to apply external source AC (activated carbon), AC and wet sand volume ratio are 500: 1, can improve and sprout
Hair rate.
The foregoing is merely the embodiment of the present invention, are not intended to limit the scope of the invention, every to utilize this hair
It is related to be directly or indirectly used in other for the equivalent structure or equivalent flow shift that bright specification and embodiment content are made
Technical field, be included within the scope of the present invention.
Claims (2)
1. a kind of wild Paris polyphylla seed dormancy in Dabie Mountain breaks method, which is characterized in that includes the following steps：
(1) skin processing and sterilizing are planted：Exosper removes, and is first impregnated for 24 hours with 25 DEG C of warm water, then fresh seeds and wet sand mixing,
Rub red exosper with the hands, Seed sterilization processing is first impregnated 1~2 minute with 75% ethyl alcohol, then sterilized 15 points with 0.2% mercuric chloride
Clock, lamination add in 50% carbendazim and keep long-term sterile, 100 DEG C or more the high-temp steam sterilizings of sand in the inside in the process；
(2) seed dormancy breaking steps：Paris polyphylla seed afterripening is totally divided into warm temperature-low temperature-warm warm three-level alternating temperature stratification processing, uses
It carries out lamination within 180 days, warms up 60 days constant temperature processing times of temperature, be made of two stages, because 40 days be a material time node, therefore
It is divided into form after-ripening early period and later stage, by there is " secondary dormancy " characteristic because of Paris polyphylla seed, it is necessary to have cryogenic thermostat processing, low temperature
Processing time is 60 days, is then transferred to warm warm intermittent warming, it is necessary to be 20 DEG C and 15 DEG C intermittent warming 40 days round the clock, into last
In the stage, warming up the vernalization of temperature constant temperature needs 20 days.
2. the wild Paris polyphylla seed dormancy in a kind of Dabie Mountain described in accordance with the claim 1 breaks method, it is characterised in that：The step
Suddenly five stages in (2) are respectively：
(1) first stage is form after-ripening early period (0-40 days)：Preceding 40 days warm layers product be to reduce endogenous aba content, and
Cause embryo development, application exogenous GA 3 has ABA antagonism during this, while the after-ripening of embryo form is completed to have and is promoted
It into effect, can apply on a small quantity, shorten form ripening time, exogenous GA 3 concentration is 10ppm, this stage needs to be warm layer
The time that 20 DEG C of degrees Celsius of processing of product need is 60 days；
(2) second stage is the form after-ripening later stage (40-60 days)：The seed after warm layer accumulates 40 days is taken out, then carries out seed
Sterilization treatment, this is the critical period that Paris polyphylla seed switchs to Dormancy breaking state from dormant state within 40-60 days, in form
It is strictly that 60-90% is kept with humidifier by 20 DEG C of completion morphology after-ripening relative humidity requirements in After-ripening, while 3-
It to stir in time within 5 days, help oxygen supply and ventilation, funginert growth, but during 40-60 days, apply exogenous GA 3,
Its GA3 concentration for the treatment of is unsuitable excessively high, it should maintain a reduced levels, concentration 100ppm, Paris polyphylla seed is by warm layer
After product processing in 60 days, embryo is cylindrical embryo from subsphaeroidalization development, and hypocotyl expands overstriking, gradually forms rhizome, completes
Form after-ripening；
(3) phase III is the physiological after-ripening stage (60-120 days)：Cool stratification induction is again this stage of key point
Need be the processing of 5 DEG C degrees Celsius of cool stratification, it is necessary to time be 60 days, apply exogenous GA 3, concentration for the treatment of 200mg/l, leaching
Bubble for 24 hours, further takes out and is admixed after drying in 1: 5 ratio through in the processed wet sand of high-temperature sterilization, being put into growth cabinet progress
Stratification, the condition of growth cabinet are set as：18 DEG C of temperature, humidity 70%, while apply external source AC (activated carbon) progress
Absorption, AC and wet sand volume ratio are 500: 1；
(4) fourth stage is the alternately alternating temperature stratification processing (120-160 days) of warm temperature：With 20 DEG C of daytime, night is handled with 15 DEG C, is needed
The time taken is 40 days or so.And applying exogenous GA 3, concentration for the treatment of 200mg/l is impregnated for 24 hours, further taken out after drying by 1: 5
Ratio admix through in the processed wet sand of high-temperature sterilization, be put into growth cabinet carry out stratification, the item of growth cabinet
Part is set as：18 DEG C of temperature, humidity 70%；
(5) the 5th stages were vernalization stage (120-160 days)：This stage needs to be at warm warm 20 DEG C degrees Celsius of constant temperature lamination
, it is necessary to the time is 20 days or so, wet sand processing is same as above reason, and Inhibiting germination substance has, but easily row unrelated with physiological after-ripening
Go out endogenous Inhibiting germination substance, it is necessary to apply external source AC (activated carbon), AC and wet sand volume ratio are 500: 1, can improve sprouting
Rate.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089378A (en) * | 2019-05-24 | 2019-08-06 | 成都市农林科学院 | A kind of efficient Paris polyphylla seed seedling-raising method |
| CN110268907A (en) * | 2019-07-29 | 2019-09-24 | 天津泰达盐碱地绿化研究中心有限公司 | A kind of loguat leaf Jia Opulus is in beach saline land introduction and acclimatization and mating system |
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| CN110089378A (en) * | 2019-05-24 | 2019-08-06 | 成都市农林科学院 | A kind of efficient Paris polyphylla seed seedling-raising method |
| CN110268907A (en) * | 2019-07-29 | 2019-09-24 | 天津泰达盐碱地绿化研究中心有限公司 | A kind of loguat leaf Jia Opulus is in beach saline land introduction and acclimatization and mating system |
| CN110268907B (en) * | 2019-07-29 | 2021-11-23 | 天津泰达盐碱地绿化研究中心有限公司 | Method for introducing, domesticating and breeding viburnum komarovii in coastal saline-alkali soil |
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