CN107987177A - 一种降血糖龙眼肉多糖的分离纯化方法 - Google Patents
一种降血糖龙眼肉多糖的分离纯化方法 Download PDFInfo
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- CN107987177A CN107987177A CN201711234594.2A CN201711234594A CN107987177A CN 107987177 A CN107987177 A CN 107987177A CN 201711234594 A CN201711234594 A CN 201711234594A CN 107987177 A CN107987177 A CN 107987177A
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- arillus longan
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- 235000000235 Euphoria longan Nutrition 0.000 title claims abstract description 165
- 150000004676 glycans Chemical class 0.000 title claims abstract description 118
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 118
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 118
- 230000002218 hypoglycaemic effect Effects 0.000 title claims abstract description 35
- 238000000746 purification Methods 0.000 title claims abstract description 17
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000002955 isolation Methods 0.000 title claims abstract description 12
- 244000139609 Euphoria longan Species 0.000 title 1
- 240000001008 Dimocarpus longan Species 0.000 claims abstract description 164
- 239000000284 extract Substances 0.000 claims abstract description 103
- 235000009508 confectionery Nutrition 0.000 claims abstract description 28
- 238000003756 stirring Methods 0.000 claims abstract description 19
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 11
- 239000012528 membrane Substances 0.000 claims abstract description 9
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- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 89
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 75
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 74
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 54
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- 239000007788 liquid Substances 0.000 claims description 23
- 239000008367 deionised water Substances 0.000 claims description 16
- 229910021641 deionized water Inorganic materials 0.000 claims description 16
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- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
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- 150000001720 carbohydrates Chemical group 0.000 description 2
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- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
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- 241001465754 Metazoa Species 0.000 description 1
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- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
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- 210000004027 cell Anatomy 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
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- 210000004292 cytoskeleton Anatomy 0.000 description 1
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
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- 230000002708 enhancing effect Effects 0.000 description 1
- 229940125532 enzyme inhibitor Drugs 0.000 description 1
- 239000002532 enzyme inhibitor Substances 0.000 description 1
- 239000000469 ethanolic extract Substances 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
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- 206010022437 insomnia Diseases 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- ZDGGJQMSELMHLK-UHFFFAOYSA-N m-Trifluoromethylhippuric acid Chemical compound OC(=O)CNC(=O)C1=CC=CC(C(F)(F)F)=C1 ZDGGJQMSELMHLK-UHFFFAOYSA-N 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
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- 235000016709 nutrition Nutrition 0.000 description 1
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- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
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- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Sustainable Development (AREA)
- Medicines Containing Plant Substances (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
样品 | IC50/ug·mL-1 |
总提取物 | 1559.7 |
石油醚萃取物 | 2236.2 |
乙酸乙酯萃取物 | 1827.7 |
正丁醇萃取物 | 1345.8 |
水萃取物 | 705.2 |
阿卡波糖(阳性对照) | 677.4 |
Claims (1)
Priority Applications (1)
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CN201711234594.2A CN107987177B (zh) | 2017-11-30 | 2017-11-30 | 一种降血糖龙眼肉多糖的分离纯化方法 |
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CN201711234594.2A CN107987177B (zh) | 2017-11-30 | 2017-11-30 | 一种降血糖龙眼肉多糖的分离纯化方法 |
Publications (2)
Publication Number | Publication Date |
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CN107987177A true CN107987177A (zh) | 2018-05-04 |
CN107987177B CN107987177B (zh) | 2020-06-30 |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110433220A (zh) * | 2019-07-30 | 2019-11-12 | 浙江师范大学 | 从龙眼核中提取α-葡萄糖苷酶抑制剂的方法 |
CN111234046A (zh) * | 2020-03-23 | 2020-06-05 | 广西壮族自治区农业科学院 | 茉莉花渣活性多糖的制备方法及其应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102070724A (zh) * | 2010-12-24 | 2011-05-25 | 广东省农业科学院农业生物技术研究所 | 一种中性龙眼多糖级分的分离方法 |
-
2017
- 2017-11-30 CN CN201711234594.2A patent/CN107987177B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102070724A (zh) * | 2010-12-24 | 2011-05-25 | 广东省农业科学院农业生物技术研究所 | 一种中性龙眼多糖级分的分离方法 |
Non-Patent Citations (2)
Title |
---|
郭立达等: "《动物细胞分离培养》", 31 August 2015, 重庆大学出版社 * |
陈彦林: ""不同干制方式龙眼果肉主要活性物质的比较"", 《"不同干制方式龙眼果肉主要活性物质的比较"》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110433220A (zh) * | 2019-07-30 | 2019-11-12 | 浙江师范大学 | 从龙眼核中提取α-葡萄糖苷酶抑制剂的方法 |
CN111234046A (zh) * | 2020-03-23 | 2020-06-05 | 广西壮族自治区农业科学院 | 茉莉花渣活性多糖的制备方法及其应用 |
CN111234046B (zh) * | 2020-03-23 | 2021-10-15 | 广西壮族自治区农业科学院 | 茉莉花渣活性多糖的制备方法及其应用 |
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