CN107981046A - A kind of method of comprehensive utilization of pineapple peel - Google Patents
A kind of method of comprehensive utilization of pineapple peel Download PDFInfo
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- CN107981046A CN107981046A CN201711217393.1A CN201711217393A CN107981046A CN 107981046 A CN107981046 A CN 107981046A CN 201711217393 A CN201711217393 A CN 201711217393A CN 107981046 A CN107981046 A CN 107981046A
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- pineapple
- pineapple peel
- yeast
- comprehensive utilization
- juice
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
- A23L31/10—Yeasts or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/22—Cysteine endopeptidases (3.4.22)
- C12Y304/22004—Bromelain (3.4.22.4)
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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Abstract
The invention discloses a kind of method of comprehensive utilization of pineapple peel, the method separates pineapple peel juice and pineapple bran by will squeeze the juice after the cleaning removal of impurities of fresh pineapple skin, pineapple bran access probiotics fermention is probiotic feed, pineapple peel juice prepares bromelain semifinished product and the carbon source material as Yeast Cultivation, also can be as the carbon source material of probiotic's culture, the yeast cultivated can be used as food yeast or yeast for animal feeds use, and the probiotics cultivated can use as the ammonia nitrogen antidote of aquaculture system, ammonia nitrogen degradation agent.The method takes full advantage of the various composition in pineapple peel, realizes a good circulation, easy to operate, is adapted to large-scale production, during will not produce pollution to environment, and avoid discarded pineapple peel pollution on the environment.
Description
Technical field
The present invention relates to technical field of biological fermentation, more particularly to a kind of method of comprehensive utilization of pineapple peel.
Background technology
Pineapple, also known as pineapple, are one of immensely popular tropical fruit (tree)s, the main plantation area in China have Guangdong, Hainan,
The provinces such as Guangxi, Taiwan, Fujian, Yunnan.The flavor of original fresh fruit is still maintained after being processed due to pineapple, thus in fruit process
It is popular in industry；But pineapple fruit is not easy to store, outside depolarization small part direct marketing, be mostly used in be processed into can and
Preserved fruit.
Pineapple peel is the leftover bits and pieces of pineapple processing, contains abundant dietary fiber, sugar, organic acid, protein etc., moisture content
85% or so, easily putrid and deteriorated, processing the problems such as causing environmental pollution not in time；The Technical Board of industrialized utilization pineapple peel
It is limited to make cattle and sheep greenfeed and extracts bromelain and pectin etc., in Chinese patent CN 101617737A, by spinach
After trailing plants skin juice, slag separation, pineapple bran adds corn flour, strain, complex enzyme, carries out prolonged fermentation manufacture feed, pineapple peel
Juice produces active dry yeast, but this method zymotechnique is complicated, and the aerobic fermentation time is too long, and mycotoxin is produced in fermentation process
Etc. harmful substance, while the product after fermentation is used for direct nutrition purposes, especially for feeding animals, and the preservation effect of product is bad, easily putrid and deteriorated.
As it can be seen that the prior art could be improved and improve.
The content of the invention
In view of in place of above-mentioned the deficiencies in the prior art, it is an object of the invention to provide a kind of comprehensive utilization side of pineapple peel
Method, it is intended to solve the limitation of industrialized utilization pineapple peel in the prior art, pineapple peel is fully changed into available thing
Matter, avoids discarded pineapple peel pollution on the environment.
In order to achieve the above object, this invention takes following technical scheme：
A kind of method of comprehensive utilization of pineapple peel, including：
A. fresh pineapple peel is cleaned into removal of impurities, with squeezer extrusion dehydration, collects pineapple peel juice and pineapple bran respectively；
B. toward addition bacillus subtilis, bacillus coagulans, candida utili in dewatered pineapple bran in step A
Bacterium, is uniformly mixed, and carries out aerobic fermentation；
C. it is close toward adding Lactobacillus acidophilus, saccharomyces cerevisiae as strain in the pineapple bran in step B after aerobic fermentation
Envelope preserves 7 days, carries out anaerobic fermentation, obtains active probiotic feed；
D. the pineapple peel juice collected in step A is centrifuged, takes supernatant, add kaolin, when stirring 1 is small, centrifugation；Sediment is
Bromelain semifinished product, in 4-6 DEG C of preservation；The sterilizing storage of pineapple peel juice clear liquid；
E. the pineapple peel juice continuous stream after the sterilizing in step D is added in the bubble type yeast culture tank containing yeast strain,
After the nutrient solution in culture tank after subsequent fermentation tank culture maturation, yeast is centrifuged, it is dry, obtain dusty yeast.
In the method for comprehensive utilization of the pineapple peel, this method, which further includes, adds the pineapple peel juice after sterilizing in step D
2000CFU/ milliliters of Lactobacillus acidophilus, 2000CFU/ milliliters of enterococcus faecalis, 5 are accessed after adding 0.5% yeast extract, 0.5% sodium chloride
Ten thousand CFU/ milliliters of bacillus coagulans, Anaerobic culturel 7 days, detection total bacteria count are packed in 2,000,000,000 CFU/ milliliter, pH value 2.8-3.2,
Ammonia nitrogen antidote in aquaculture system, ammonia nitrogen degradation agent.
In the method for comprehensive utilization of the pineapple peel, the pineapple peel after being cleaned in the step A is set by series connection respectively
Some screw extrusion dewaterers put, the dewatered moisture content of pineapple peel are 70%.
In the method for comprehensive utilization of the pineapple peel, the strain amount accessed in the step B is with the weight of pineapple bran
Amount is calculated as：1000000 CFU/ grams of bacillus subtilises, 400,000 CFU/ grams of bacillus coagulanses, 1,000,000 CFU/ grams of Candida utilis ferment
Female bacterium.
In the method for comprehensive utilization of the pineapple peel, the temperature of aerobic fermentation is 25-50 DEG C in the step B, hair
The ferment time is 10-16h.
In the method for comprehensive utilization of the pineapple peel, the strain amount that is accessed in the anaerobic fermentation in the step C with
The weight of pineapple bran is calculated as：2000CFU/ grams of S. cervisiae, 4000CFU/ grams of Lactobacillus acidophilus.
In the method for comprehensive utilization of the pineapple peel, the environment temperature of the anaerobic fermentation in the step C is 25-35
℃。
In the method for comprehensive utilization of the pineapple peel, anaerobic fermentation occurs with air bleeding valve in the step C
In sealed plastic bag.
In the method for comprehensive utilization of the pineapple peel, mixing speed in the step D turns/min for 50-60, and two
The speed of secondary centrifugation is that 3000-4000 turns/min.
In the method for comprehensive utilization of the pineapple peel, yeast is spray-dried by spray drying tower in the step E,
Temperature of charge is at 80-100 DEG C when control is dry.
Beneficial effect：
The present invention provides a kind of method of comprehensive utilization of pineapple peel, the method after the cleaning removal of impurities of fresh pineapple skin by will squeeze
Juice separates pineapple peel juice and pineapple bran, and pineapple bran access probiotics fermention is probiotic feed, and pineapple peel juice is prepared as spinach
Trailing plants protease semifinished product and the carbon source production dusty yeast as Yeast Cultivation or the carbon source material as probiotic's culture, are cultivated
Yeast food yeast or yeast for animal feeds can be used as to use, the probiotics cultivated can be as the ammonia nitrogen solution of aquaculture system
Toxic agent, ammonia nitrogen degradation agent use.The method takes full advantage of the various composition in pineapple peel, realizes a good circulation,
It is easy to operate, be adapted to large-scale production, during will not produce pollution to environment, and avoid discarded pineapple peel and environment made
Into pollution.
Brief description of the drawings
Fig. 1 is the flow chart of the method for comprehensive utilization of the pineapple peel provided by the invention.
Embodiment
The present invention provides a kind of method of comprehensive utilization of pineapple peel, to make the purpose of the present invention, technical solution and effect more
Add clear, clear and definite, the present invention is described in more detail for the embodiment that develops simultaneously referring to the drawings.It is it should be appreciated that described herein
Specific embodiment only to explain the present invention, be not intended to limit the present invention.
Referring to Fig. 1, the present invention provides a kind of method of comprehensive utilization of pineapple peel, including：
A. fresh pineapple peel is cleaned into removal of impurities, with squeezer extrusion dehydration, collects pineapple peel juice and pineapple bran respectively；Specifically
Ground, fresh pineapple peel first dispose the wood on surface, metal impurities, and the impurity such as silt are washed in sink is put into；
B. toward addition bacillus subtilis, bacillus coagulans, candida utili in dewatered pineapple bran in step A
Bacterium, is uniformly mixed, and carries out aerobic fermentation；
C. it is close toward adding Lactobacillus acidophilus, saccharomyces cerevisiae as strain in the pineapple bran in step B after aerobic fermentation
Envelope preserves 7 days, carries out anaerobic fermentation, obtains active probiotic feed；
D. the pineapple peel juice collected in step A is centrifuged, takes supernatant, add kaolin, when stirring 1 is small, centrifugation；Sediment is
Bromelain semifinished product, in 4-6 DEG C of preservation；The sterilizing storage of pineapple peel juice clear liquid；Kaolin has good suspension, absorption
Property and caking property, are dispersed in water and are difficult to precipitate, pineapple peel juice clear liquid can effectively be adsorbed by being suspended in pineapple peel juice clear liquid
In bromelain, after centrifugation as precipitation separated；The pineapple peel juice clear liquid for isolating bromelain goes out by Pasteur
Stored after bacterium, can be as the carbon source material of Yeast Cultivation.
E. it is added to the drum containing yeast strain using the pineapple peel juice after the sterilizing in step D as culture medium carbon source continuous stream
In bubble yeast culture tank, after the nutrient solution in culture tank after subsequent fermentation tank culture maturation, yeast is centrifuged, it is dry,
Obtain dusty yeast；Yeast also needs to nutriment, the obtained dusty yeasts such as addition urea, phosphoric acid in incubation can conduct
Food yeast or fodder yeast use, and supplemented with the required strain of probiotic feed is prepared, realize pineapple peel component
Recycle, reduce and extra additive is relied on, reduce the cost of production；Waste water after centrifugation is through biochemical treatment heel row
Put, avoid pollution of the waste water to environment.
Preferably, the method for comprehensive utilization of the pineapple peel, which further includes, adds the pineapple peel juice after sterilizing in step D
2000CFU/ milliliters of Lactobacillus acidophilus, 2000CFU/ milliliters of enterococcus faecalis, 50,000 are accessed after 0.5% yeast extract, 0.5% sodium chloride
CFU/ milliliters of bacillus coagulanses, Anaerobic culturel 7 days, detection total bacteria count are packed, obtained in 2,000,000,000 CFU/ milliliter, pH value 2.8-3.2
Ammonia nitrogen antidote, ammonia nitrogen degradation agent in aquaculture system.
Specifically, the pineapple peel after being cleaned in the step A is dehydrated by some screw extrusions being arranged in series respectively
Machine, the dewatered moisture content of pineapple peel are 70%；In the present embodiment, the pineapple peel after cleaning continues through 3 screw extrusions
Dewaterer, and the pineapple peel juice deviate from every time is collected, under normal circumstances, by the pineapple peel after 3 screw extrusion dewaterers
Moisture can reach 70% or so, the moisture is adapted to the fermentation of follow-up probiotic feed, and moisture is excessive, can disappear
More strains are consumed, cause unnecessary waste；Moisture is too low, there is provided not strain fermentation required nutritional ingredient.
Specifically, the strain amount accessed in the step B is calculated as with the weight of pineapple bran：1000000 CFU/ grams it is withered
Careless bacillus, 400,000 CFU/ grams of bacillus coagulanses, 1,000,000 CFU/ grams of candida utili bacterium；Above-mentioned strain miscellaneous bacteria contains
Measure low, dosage is few, and enzymolysis and fermentation can degrade anti-nutritional factors and poisonous and hazardous material in feedstuff, improve feed
Quality, reduce feed-weight ratio；The growth of effective bacterium in animal intestinal tract can be effectively inhibited after being fed in vivo by animal, is changed
Kind intestine microenvironment, promotes intestinal growth, strengthens function of intestinal canal, promotes digesting and assimilating for feed, can effectively improve disconnected
Milk piglet due to stress, caused by allergic reaction diarrhoea with dislocation of bacillary groups phenomenon, the quantity of above-mentioned access strain can allow bacterium
Kind quickly bred under aerobic environment, competitively suppress the breeding of harmful bacteria, at the same can shorten aerobic fermentation when
Between, avoid the growth of mould；For dosage excessively it will cause waste, dosage is very few, attenuation degree deficiency, enzymolysis be not thorough, it is necessary to
Fermentation time length, easily produce the harmful bacteria such as mould.
Preferably, the temperature of aerobic fermentation is 25-50 DEG C in the step B, fermentation time 10-16h；In 25-50
DEG C environmental condition under, most useful for the growth of the probiotics in material, but in the fermentation process of probiotics can heat release, therefore,
Also want the temperature of control material should not be excessive, temperature is excessive to inactivate strain；Environment temperature is too low, bacterial activity deficiency, meeting
Cause attenuation degree too low.
Specifically, the strain amount accessed in the anaerobic fermentation in the step C is calculated as with the weight of pineapple bran：
2000CFU/ grams of S. cervisiae, 4000CFU/ grams of Lactobacillus acidophilus；Material carries out anaerobism hair under the action of the strain
Ferment, produces lactic acid, is being acidified material, can be preserved for a long time in the environment of anaerobic.
Preferably, the environment temperature of the anaerobic fermentation in the step C is 25-35 DEG C；It is most suitable for detesting at the temperature
The growth of the strain in aerobe fermentation, shortens the time of fermentation.
Preferably, anaerobic fermentation occurs in the sealed plastic bag with air bleeding valve in the step C；Hermetic bag is favourable
In the formation of anaerobic environment, so as to be conducive to growth and breeding and the metabolism of lactic acid bacteria, the metabolism such as more lactic acid and enzyme production is produced
Thing；Hermetic bag can be expanded according to the gas that fermentation process produces, and air bleeding valve can discharge the gas produced in fermentation process,
Prevent gas excessive and burst hermetic bag；External environment can be neutralized by air bleeding valve elder generation balanced seal bag after fermentation
Air pressure, be conducive to material long-time preserve.
Preferably, the mixing speed in the step D turns/min for 50-60, and the speed centrifuged twice is 3000-
4000 turns/min, it is highly preferred that mixing speed is 60 turns/min, this rotating speed is uniformly dispersed beneficial to kaolin, adsorbs pineapple egg
White enzyme, the too fast easily blistering, and waste the unnecessary energy of rotating speed, rotating speed is excessively slow, and kaolin can settle, it is impossible to fully inhale
Bromelain in attached pineapple peel juice.
Preferably, yeast is spray-dried by using spray drying tower in the step E, temperature of charge when control is dry
80-100℃。
Embodiment 1
Fresh pineapple peel is cleaned out into wood, metal impurities, then is put into sink and washes the impurity such as silt；Use conveyer belt
It is sent in 3 screw extrusion being used in series dewaterers, the pineapple peel juice deviate from dewaterer is collected respectively, after collecting dehydration
Pineapple bran, weigh, access 1,000,000 CFU/ grams of bacillus subtilises, 400,000 CFU/ grams of bacillus coagulanses, 1,000,000 CFU/
Gram production protein vacation saccharomycete, be uniformly mixed, be placed in aerobic fermentation 16h in 25 DEG C of environment；Material after aerobic fermentation accesses again
4000CFU/ grams of Lactobacillus acidophilus, 2000CFU/ grams of saccharomyces cerevisiae, are uniformly mixed, and are fitted into the hermetic bag with air bleeding valve,
It is 25 DEG C to control environment temperature, and temperature of charge is less than 40 DEG C, ferments 7 days, obtains probiotic feed；The pineapple that pineapple peel dehydration is collected
Skin juice is centrifuged with horizontal centrifuge, takes the clear liquid after centrifugation, alukalin is added, with mixer with the rotating speed of 60 turns/min
1h, 4000 turns/min centrifugations are stirred, obtained sediment, as bromelain semifinished product, is produced in 4 DEG C of refrigerations for purifying
Bromenzyme；Sterilized with pasteurization to the clear liquid after centrifugation, the clear liquid continuous stream after sterilizing is added to bubbling
In the yeast culture tank of formula, as the carbon source of Yeast Cultivation, flow acceleration is to contain in the zymotic fluid ensured in yeast culture tank
0.5% glucose, and be used as nutrient solution toward urea, phosphoric acid etc. is added in zymotic fluid, after yeast fermenting-ripening, centrifugation,
The lotion separated is spray-dried, and 80 DEG C of temperature of charge when control is dry, obtains dried yeast powder, the dried yeast powder of gained can be made
Used for food yeast or fodder yeast；
The strain amount of above-mentioned access is calculated with the weight of pineapple bran.
Embodiment 2
Fresh pineapple peel is cleaned out into wood, metal impurities, then is put into sink and washes the impurity such as silt；Use conveyer belt
It is sent in 3 screw extrusion being used in series dewaterers, the pineapple peel juice deviate from dewaterer is collected respectively, after collecting dehydration
Pineapple bran, weigh, access 1,000,000 CFU/ grams of bacillus subtilises, 400,000 CFU/ grams of bacillus coagulanses, 1,000,000 CFU/
Gram production protein vacation saccharomycete, be uniformly mixed, be placed in aerobic fermentation 15h in 50 DEG C of environment；Material after aerobic fermentation accesses again
4000CFU/ grams of Lactobacillus acidophilus, 2000CFU/ grams of saccharomyces cerevisiae, are uniformly mixed, and are fitted into the hermetic bag with air bleeding valve,
It is 35 DEG C to control environment temperature, and temperature of charge is less than 40 DEG C, ferments 7 days, obtains probiotic feed；The pineapple that pineapple peel dehydration is collected
Skin juice is centrifuged with horizontal centrifuge, takes the clear liquid after centrifugation, alukalin is added, with mixer with the rotating speed of 50 turns/min
1h, 4000 turns/min centrifugations are stirred, obtained sediment, as bromelain semifinished product, is produced in 5 DEG C of refrigerations for purifying
Bromenzyme；Sterilized with pasteurization to the clear liquid after centrifugation, the clear liquid continuous stream after sterilizing is added to bubbling
In the yeast culture tank of formula, as the carbon source of Yeast Cultivation, flow acceleration is to contain in the zymotic fluid ensured in yeast culture tank
0.5% glucose, and be used as nutrient solution toward urea, phosphoric acid etc. is added in zymotic fluid, after yeast fermenting-ripening, centrifugation,
Centrifuge lotion out to be dried with spray drying tower, 100 DEG C of temperature of charge when control is dry, obtains dried yeast powder, gained
Dried yeast powder can be used as food yeast or fodder yeast to use.
The strain amount of above-mentioned access is calculated with the weight of pineapple bran.
Embodiment 3
Fresh pineapple peel is cleaned out into wood, metal impurities, then is put into sink and washes the impurity such as silt；Use conveyer belt
It is sent in 3 screw extrusion being used in series dewaterers, the pineapple peel juice deviate from dewaterer is collected respectively, after collecting dehydration
Pineapple bran, weigh, access 1,000,000 CFU/ grams of bacillus subtilises, 400,000 CFU/ grams of bacillus coagulanses, 1,000,000 CFU/
Gram production protein vacation saccharomycete, be uniformly mixed, be placed in aerobic fermentation 14h in 30 DEG C of environment；Material after aerobic fermentation accesses again
4000CFU/ grams of Lactobacillus acidophilus, 2000CFU/ grams of saccharomyces cerevisiae, are uniformly mixed, and are fitted into the hermetic bag with air bleeding valve,
It is 32 DEG C to control environment temperature, and temperature of charge is less than 40 DEG C, ferments 7 days, obtains probiotic feed；The pineapple that pineapple peel dehydration is collected
Skin juice is centrifuged with horizontal centrifuge, takes the clear liquid after centrifugation, alukalin is added, with mixer with the rotating speed of 55 turns/min
1h, 3000 turns/min centrifugations are stirred, obtained sediment, as bromelain semifinished product, is produced in 4 DEG C of refrigerations for purifying
Bromenzyme；Sterilized with pasteurization to the clear liquid after centrifugation, the clear liquid continuous stream after sterilizing is added to bubbling
In the yeast culture tank of formula, as the carbon source of Yeast Cultivation, flow acceleration is to contain in the zymotic fluid ensured in yeast culture tank
0.5% glucose, and be used as nutrient solution toward urea, phosphoric acid etc. is added in zymotic fluid, after yeast fermenting-ripening, centrifugation,
It is spray-dried to separate lotion, obtains dried yeast powder, the dried yeast powder of gained can be used as food yeast or fodder yeast to use.
The strain amount of above-mentioned access is calculated with the weight of pineapple bran.
Embodiment 4
Fresh pineapple peel is cleaned out into wood, metal impurities, then is put into sink and washes the impurity such as silt；Use conveyer belt
It is sent in 3 screw extrusion being used in series dewaterers, the pineapple peel juice deviate from dewaterer is collected respectively, after collecting dehydration
Pineapple bran, weigh, access 1,000,000 CFU/ grams of bacillus subtilises, 400,000 CFU/ grams of bacillus coagulanses, 1,000,000 CFU/
Gram production protein vacation saccharomycete, be uniformly mixed, be placed in aerobic fermentation 12h in 40 DEG C of environment；Material after aerobic fermentation accesses again
4000CFU/ grams of Lactobacillus acidophilus, 20,000 CFU/ grams of saccharomyces cerevisiaes, are uniformly mixed, and are fitted into the hermetic bag with air bleeding valve, control
Environment temperature processed is 30 DEG C, and temperature of charge is less than 40 DEG C, ferments 7 days, obtains probiotic feed；The pineapple peel that pineapple peel dehydration is collected
Juice is centrifuged with horizontal centrifuge, takes the clear liquid after centrifugation, is added alukalin, is stirred with mixer with the rotating speed of 55 turns/min
1h, 3500 turns/min centrifugations are mixed, obtained sediment, as bromelain semifinished product, spinach is produced for purifying in 6 DEG C of refrigerations
Trailing plants protease preparation；Sterilized with pasteurization to the clear liquid after centrifugation, the clear liquid continuous stream after sterilizing is added to bubble type
Yeast culture tank in, as the carbon source of Yeast Cultivation, flow acceleration for ensure yeast culture tank in zymotic fluid in contain
0.5% carbon source, and be used as nutrient solution toward urea, phosphoric acid etc. is added in zymotic fluid, after yeast fermenting-ripening, centrifuge, divide
From lotion through roller drier drying, 80 °C of control material temperature, dry ferment is obtained, dusty yeast, the dry ferment of gained are obtained after crushing
Powder can be used as food yeast or fodder yeast to use.
The strain amount of above-mentioned access is calculated with the weight of pineapple bran.
Embodiment 5
Fresh pineapple peel is cleaned out into wood, metal impurities, then is put into sink and washes the impurity such as silt；Use conveyer belt
It is sent in 3 screw extrusion being used in series dewaterers, the pineapple peel juice deviate from dewaterer is collected respectively, after collecting dehydration
Pineapple bran, weigh, 10,000,000 CFU/ grams of bacillus subtilises of access, 1,000,000 CFU/ grams of production protein vacation saccharomycete, mixing is equal
It is even, it is placed in aerobic fermentation 10h in 45 DEG C of environment；Material after aerobic fermentation access again 4000CFU/ grams of Lactobacillus acidophilus,
2000CFU/ grams of saccharomyces cerevisiae, is uniformly mixed, and is fitted into the hermetic bag with air bleeding valve, and it is 28 DEG C to control environment temperature, material
Temperature is less than 40 DEG C, ferments 7 days, obtains probiotic feed；The pineapple peel juice that pineapple peel dehydration is collected is centrifuged with horizontal centrifuge, is taken
Clear liquid after centrifugation, adds alukalin, stirs 1h with mixer with the rotating speed of 60 turns/min, 4000 turns/min centrifugations, obtain
The sediment arrived, as bromelain semifinished product, Bromenzyme is produced for purifying in 4 DEG C of refrigerations；Gone out with Pasteur
Bacterium method sterilizes the clear liquid after centrifugation, and the clear liquid continuous stream after sterilizing is added in the yeast culture tank of bubble type, as ferment
The carbon source of mother's culture, flow acceleration is ensures in zymotic fluid containing 0.5% carbon source, and toward addition urea, phosphoric acid in zymotic fluid
Deng being used as nutrient solution, after yeast fermenting-ripening, centrifuge, separation lotion is dried with roller drier, 90 ° of control material temperature
C, after crushed, obtains dried yeast powder, the dried yeast powder of gained can be used as food yeast or fodder yeast to use.
The strain amount of above-mentioned access is calculated with the weight of pineapple bran.
Embodiment 6
Fresh pineapple peel is cleaned out into wood, metal impurities, then is put into sink and washes the impurity such as silt；Use conveyer belt
It is sent in 3 screw extrusion being used in series dewaterers, the pineapple peel juice deviate from dewaterer is collected respectively, after collecting dehydration
Pineapple bran, weigh, 10,000,000 CFU/ grams of bacillus subtilises of access, 1,000,000 CFU/ grams of production protein vacation saccharomycete, mixing is equal
It is even, it is placed in aerobic fermentation 12h in 40 DEG C of environment；Material after aerobic fermentation access again 4000CFU/ grams of Lactobacillus acidophilus,
2000CFU/ grams of saccharomyces cerevisiae, is uniformly mixed, and is fitted into the hermetic bag with air bleeding valve, and it is 30 DEG C to control environment temperature, material
Temperature is less than 40 DEG C, ferments 7 days, obtains probiotic feed；The pineapple peel juice that pineapple peel dehydration is collected is centrifuged with horizontal centrifuge, is taken
Clear liquid after centrifugation, adds alukalin, stirs 1h with mixer with the rotating speed of 60 turns/min, 4000 turns/min centrifugations, obtain
The sediment arrived, as bromelain semifinished product, Bromenzyme is produced for purifying in 4 DEG C of refrigerations；Gone out with Pasteur
Bacterium method sterilizes the clear liquid after centrifugation, accesses and presses after 0.5% yeast extract of pineapple peel juice addition, 0.5% sodium chloride after sterilizing
According to the 2000CFU/ milliliters Lactobacillus acidophilus of the volume calculating of pineapple peel juice, 2000CFU/ milliliters of enterococcus faecalis, 50,000 CFU/ millis
Bacillus coagulans, Anaerobic culturel 7 days are risen, detection total bacteria count is packed, aquatic products is prepared and supports in 2,000,000,000 CFU/ milliliter, PH 3
Grow ammonia nitrogen antidote in water body, ammonia nitrogen degradation agent.
The strain amount of above-mentioned access is calculated with the weight of pineapple bran（Except special instruction）.
Method described in above-described embodiment 1-6 realizes the comprehensive utilization of pineapple peel, and pineapple bran is used as preparing probiotics
Feed, the palatability of prepared probiotic feed is good, can prevent the breeding of harmful bacteria, improves animal gastrointestinal environment；Pineapple
Bromelain semifinished product prepared by skin juice can be used for purifying production Bromenzyme, and pineapple juice divides in embodiment 1-5
Clear liquid from after is used as the carbon source of Yeast Cultivation, and obtained yeast may be used as food yeast or fodder yeast uses, for system
Standby probiotic feed provides strain, realizes a good circulation, reduces the dependence to extra additive, change give up into
Treasured, reduces the waste of resource；Pineapple peel juice after sterilizing in embodiment 6 is used for ammonia nitrogen antidote, the ammonia for producing aquaculture
Nitrogen degradation agent, takes full advantage of the value of pineapple peel juice；The upgrowth situation for the strain cultivated is good, and survival rate is high.
It is understood that for those of ordinary skills, can be with technique according to the invention scheme and its hair
Bright design is subject to equivalent substitution or change, and all these changes or replacement should all belong to the guarantor of appended claims of the invention
Protect scope.
Claims (10)
- A kind of 1. method of comprehensive utilization of pineapple peel, it is characterised in that including：A. fresh pineapple peel is cleaned into removal of impurities, with squeezer extrusion dehydration, collects pineapple peel juice and pineapple bran respectively；B. toward addition bacillus subtilis, bacillus coagulans, candida utili in dewatered pineapple bran in step A Bacterium, is uniformly mixed, and carries out aerobic fermentation；C. it is close toward adding Lactobacillus acidophilus, saccharomyces cerevisiae as strain in the pineapple bran in step B after aerobic fermentation Envelope preserves 7 days, carries out anaerobic fermentation, obtains active probiotic feed；D. the pineapple peel juice collected in step A is centrifuged, takes supernatant, add kaolin, when stirring 1 is small, centrifugation；Sediment is Bromelain semifinished product, in 4-6 DEG C of preservation；The sterilizing storage of pineapple peel juice clear liquid；E. the pineapple peel juice continuous stream after sterilizing in step D is added in the bubble type yeast culture tank containing yeast strain, treated Nutrient solution in culture tank centrifuges yeast after subsequent fermentation tank culture maturation, dry, obtains dusty yeast.
- 2. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that this method is further included step D Pineapple peel juice after middle sterilizing add access after 0.5% yeast extract, 0.5% sodium chloride 2000CFU/ milliliters of Lactobacillus acidophilus, 2000CFU/ milliliters of enterococcus faecalis, 50,000 CFU/ milliliters of bacillus coagulans, Anaerobic culturel 7 days, detection total bacteria count is in 2,000,000,000 CFU/ Milliliter, pH value 2.8-3.2, packaging, obtains ammonia nitrogen antidote in aquaculture system, ammonia nitrogen degradation agent.
- 3. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that after being cleaned in the step A For pineapple peel respectively by some screw extrusion dewaterers being arranged in series, the dewatered moisture content of pineapple peel is 70%.
- 4. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that accessed in the step B Strain amount is calculated as with the weight of pineapple bran：1000000 CFU/ grams of bacillus subtilises, 400,000 CFU/ grams of bacillus coagulanses, 1000000 CFU/ grams of candida utili bacterium.
- 5. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that aerobic hair in the step B The temperature of ferment is 25-50 DEG C, fermentation time 10-16h.
- 6. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that the anaerobism in the step C The strain amount accessed in fermentation is calculated as with the weight of pineapple bran：2000CFU/ grams of S. cervisiae, 4000CFU/ grams of acidophilus Bacillus acidi lactici.
- 7. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that the anaerobism in the step C The environment temperature of fermentation is 25-35 DEG C.
- 8. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that anaerobism is sent out in the step C Ferment occurs in the sealed plastic bag with air bleeding valve.
- 9. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that the stirring in the step D Speed turns/min for 50-60, and the speed centrifuged twice is that 3000-4000 turns/min.
- 10. the method for comprehensive utilization of pineapple peel according to claim 1, it is characterised in that yeast leads in the step Spray drying tower spray drying is crossed, temperature of charge is at 80-100 DEG C when control is dry.
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Application publication date: 20180504 |
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| RJ01 | Rejection of invention patent application after publication |