CN107926675A - A kind of implantation methods of selenium-rich water spinach - Google Patents
A kind of implantation methods of selenium-rich water spinach Download PDFInfo
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- CN107926675A CN107926675A CN201711343910.XA CN201711343910A CN107926675A CN 107926675 A CN107926675 A CN 107926675A CN 201711343910 A CN201711343910 A CN 201711343910A CN 107926675 A CN107926675 A CN 107926675A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention discloses a kind of implantation methods of selenium-rich water spinach, using water planting mode, utilize ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, potassium dihydrogen phosphate, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, sodium selenate, copper sulphate, ferrous sulfate, disodium ethylene diamine tetraacetate, sodium selenite, glycine, Tyiamine Hd element, pyridoxine hydrochloride, Yan acid, inositol, folic acid, biotin, sucrose, agar prepares culture medium, stem tuber is inserted into culture medium and is bred, by water spinach in growth period Additional nutrient solution, meet the requirement of each different stages of growth of water spinach, so as to obtain high yield, good selenium-rich water spinach.
Description
Technical field
The present invention relates to growing vegetables technical field, and in particular to a kind of implantation methods of selenium-rich water spinach.
Background technology
Water spinach, original name water spinach, also known as rattan vine vegetable, burdock, logical heart dish, it is not in the mood for dish, urn dish, empty cylinder dish, bengal dayflower herb, successively
Dish.Belong to the system of fibrous root, eggplant mesh, Angiospermae, Dicotyledoneae, Convolvulaceae, is sweet potato race.The higher air of hollow dish happiness is wet
The illumination of degree, damp soil and abundance, but it is also relatively strong to the adaptability of dense planting, and water spinach is not stringent to edaphic condition requirement,
But because of its manurial-philous happiness water, still preferably the strong soil of relatively more viscous weight, retain water and nutrients power.The blade tip of water spinach is a large amount of and promptly gives birth to
Long, fertilizer requirement is big, and resistance to fertility is strong, especially big to the requirement of nitrogenous fertilizer.
Water spinach contain abundant provitamin A, B family vitamin, vitamin C and nicotinic acid and protein, fat, calcium, phosphorus,
Iron etc., its vitamin B1 content are 8 times of tomato, and calcium content is 12 times of tomato.Water spinach, which has, promotes enterocinesia, defaecation solution
The effect of malicious；The acidity of enteron aisle can be reduced after food, prevents the bacterial flora imbalance in enteron aisle, it is beneficial to giving protection against cancer；Can also clean the teeth preventing decayed tooth
Except halitosis, body building and beauty treatment, can be rated as cosmetic good merchantable brand.In addition, infection can be prevented, sunstroke prevention is antipyretic, cool blood toxin expelling, prevention dysentery.
Selenium is the trace element that a kind of human body needs.Selenium and vitamin E are both that (peroxide is to human body for anti-peroxidation material
It is harmful), there is therapeutic effect to lacking underfed children caused by protein, there is resistance to some possible carcinogens
Effect.The issuable disease of human body selenium deficiency：Energy deficiency malnutrition, blood hemolytic anemia, Keshan disease, Kaschin-Beck disease, height
Blood pressure, ischemic heart disease, hepatic sclerosis, pancreatitis, fibroma, cancer, myomata, not pregnant disease, diabetes, cataract etc..
Chinese patent《A kind of cultural method rich in Organic Chromium water spinach》(number of patent application：CN201610504459.4)
Disclose a kind of implantation methods of water spinach, including presoaking and germinating；Selection of land site preparation；Sowing；Field management；Harvesting.This method can be with
Effectively improve the content of Organic Chromium in water spinach, add the nutritive value of water spinach, while the hollow dish leaf tender stem planted is crisp,
Eating mouth feel is good.Research report at present to trace elements of selenium beneficial to human body in water spinach is less, opposite plantation lack of standardization
Easily cause soil pollution.
The content of the invention
To solve the above-mentioned problems, the present invention provides a kind of implantation methods of selenium-rich water spinach, using the mode of water planting,
By adding the nutriment needed for plant growth, to obtain high yield, good selenium-rich water spinach product.
The present invention can be achieved through the following technical solutions：
A kind of implantation methods of selenium-rich water spinach, comprise the following steps：
(1) slot device is planted：Build long 45-50cm, width 30-40cm, the planting groove of high 15-17cm, paving plastic film in groove,
Make field planting plate with the plastic foam plate more smaller than planting groove, prick hole on field planting plate, embark on journey, into column distribution；
(2) culturing and transplanting seedlings：Water spinach tender stem is taken, is cut into small pieces after cleaning water suction, the small of 0.2-0.5cm long is cut into after disinfection
Section, breeds on culture medium, keeps 25 DEG C of temperature, and propagation goes to and culture of rootage is carried out on root media after 1 month, 1 month
After take out under natural lighting, indoor placement 3d washes the culture medium on root system off, then cuttage will be colonized plate on field planting plate
It is placed in planting groove, cuttings base portion is immersed in water；
(3) seedling management：Recirculating nutrient solution 2-3 times daily, circulates 20-30min, water spinach vigorous period 7- every time
10d supplements a moisture and nutrient solution, and every batch of replacement one time of nutrition liquid, changes liquid measure as 50%；
(4) prevention and control of plant diseases, pest control：1 Buddhist nun is sprayed per 7d and imitates spirit 1:1:200 times of liquid, it is 3-4 times continuous.
The water spinach kind is that the big bone in Guangdong is clear, leafiness obstructs in vain, spire leads to one kind in dish.
Upper step (2) culture medium is：Ammonium nitrate 720-750mg/L, potassium nitrate 950-1000mg/L, calcium chloride 166-
200mg/L, magnesium sulfate 185-200mg/L, potassium dihydrogen phosphate 68-80mg/L, boric acid 10-20mg/L, manganese sulfate 25-40mg/L,
Zinc sulfate 10-20mg/L, sodium molybdate 0.25-0.5mg/L, sodium selenate 0.25-0.5mg/L, copper sulphate 0.025-0.05mg/L, sulphur
Sour ferrous iron 27.8-35mg/L, disodium ethylene diamine tetraacetate 37.3-45mg/L, sodium selenite 392-550 μ g/ml, glycine 2-
5mg/L, Tyiamine Hd element 0.5-1mg/L, pyridoxine hydrochloride 0.5-1mg/L, Yan acid 0.5-1mg/L, inositol 100-120mg/L,
Folic acid 0.5-1mg/L, biotin 0.05-0.1mg/L, sucrose 20-22g/L, agar 7.8-8.5g/L.
The pH of the culture medium is 5.5-6.0.
Upper step (2) root media is：Potassium nitrate 1900-2000mg/L, ammonium nitrate 1650-1800mg/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 170-200mg/L, magnesium sulfate 370-400mg/L, calcium chloride 440-460mg/L, potassium iodide 0.83-1mg/L, boric acid 6.3-
8.5mg/L, manganese sulfate 22.3-28mg/L, zinc sulfate 8.6-10mg/L, sodium molybdate 0.25-0.5mg/L, copper sulphate 0.025-
0.05mg/L, cobalt chloride 0.025-0.05mg/L, disodium ethylene diamine tetraacetate 37.3-40mg/L, ferrous sulfate 27.8-33mg/
L, inositol 100-120mg/L, glycine 2-5mg/L, Tyiamine Hd element 0.1-0.5mg/L, puridoxine hydrochloride 0.5-1mg/L, Yan
Sour 0.5-1mg/L, sucrose 30-40g/L, agar 7-8.2g/L, IBA2.0-5.0mg/L, NAA0.05-0.1mg/L, AC0.002-
0.005%.
The pH of the root media is 5.8-6.3.
The preparation method of upper step (2) culture medium is：Agar, sucrose are added to the water, heating stirring is dissolved to liquid and is in
Translucent, it is uniform to add other material mixings, then adds distilled water to be settled to 1000ml, with 1mol/LNaOH solution adjust pH to
5.5。
The preparation method of the root media, is made minimal medium, adds IBA, NAA, AC and stir in aforementioned manners
Mix uniformly, be settled to 1000ml, pH to 5.8 is adjusted with 1mol/LNaOH solution.
Upper step (3) nutrient solution is：Calcium nitrate 1122-1200mg/L, potassium nitrate 910-950mg/L, potassium dihydrogen phosphate
272-300mg/L, ammonium nitrate 40-50mg/L, magnesium sulfate 247-280mg/L, boric acid 1.2-1.5mg/L, manganese sulfate 0.8-1mg/
L, zinc sulfate 0.09-1.2mg/L, copper sulphate 0.1-0.3mg/L, chelant state iron 4.2-6.8mg/L, ferrous sulfate 27.8-35mg/
L, disodium ethylene diamine tetraacetate 37.3-45mg/L, sodium selenate 46-58 μ g/L；
The nutrient solution pH is 5.6-7.0.
The present invention possesses following beneficial effect：
The present invention relates to a kind of implantation methods of selenium-rich water spinach, cultivate no clay contaminated using water culture, do not apply
Fertilizer, is also less likely to occur pest and disease damage propagation, has the characteristics that easy to operate, growth cycle is short, yield is high, nuisanceless.
The present invention plants water spinach using the mode of water planting, by adjusting nutrient solution prescription, concentration, various good nutrients
Ratio, acid-base value etc. meet the requirement of each different stages of growth of water spinach, so as to be conducive to the growth of water spinach, reach fast
Speed, high yield, high-quality and other effects.
The present invention utilizes the mode of tissue cultivating and seedling, adds selenium element in the medium, the callus of tender stem passes through
The nutritional ingredient in culture medium is absorbed to be enriched with selenium source, and plant hormone-auxin, callus induction are with the addition of in root media
The significant effect that roots is organized, and it is fast to take root, rooting rate is high, and during plantlet of transplant, root system damage is small, is survived after plantlet of transplant
It hurry up, production cost can be reduced by being cultivated using culture medium, reduce loss caused by seed seedling-raising.
Embodiment
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention easy to understand, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of implantation methods of selenium-rich water spinach of the present embodiment, mainly include the following steps that：
(1) slot device is planted：Build long 47.5cm, width 35cm, the planting groove of high 16cm, paving plastic film in groove, with than kind
Plant the smaller plastic foam plate of groove and make field planting plate, prick hole on field planting plate, embark on journey, into column distribution；
(2) culturing and transplanting seedlings：Water spinach tender stem is taken, is cut into small pieces after cleaning water suction, the segment of 0.35cm long is cut into after disinfection,
Breed on culture medium, keep 25 DEG C of temperature, propagation goes to and culture of rootage is carried out on root media, after 1 month after 1 month
Take out under natural lighting, indoor placement 3d, wash the culture medium on root system off, then cuttage puts field planting plate on field planting plate
In planting groove, cuttings base portion is set to be immersed in water；
(3) seedling management：Recirculating nutrient solution 2 times daily, circulates 25min, water spinach vigorous period 8.5d supplements every time
Moisture and nutrient solution, every batch of replacement one time of nutrition liquid, change liquid measure as 50%；
(4) prevention and control of plant diseases, pest control：1 Buddhist nun is sprayed per 7d and imitates spirit 1:1:200 times of liquid, continuous 3 times.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the water spinach kind be the big bone in Guangdong is clear, leafiness obstructs in vain,
Spire leads to one kind in dish.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the root media described in step (2) are：Potassium nitrate
1950mg/L, ammonium nitrate 1725mg/L, potassium dihydrogen phosphate 185mg/L, magnesium sulfate 385mg/L, calcium chloride 450mg/L, potassium iodide
0.915mg/L, boric acid 7.4mg/L, manganese sulfate 22.3-28mg/L, zinc sulfate 9.3mg/L, sodium molybdate 0.375mg/L, copper sulphate
0.0375mg/L, cobalt chloride 0.0375mg/L, disodium ethylene diamine tetraacetate 38.65mg/L, ferrous sulfate 30.4mg/L, inositol
110mg/L, glycine 3.5mg/L, Tyiamine Hd element 0.3mg/L, puridoxine hydrochloride 0.75mg/L, Yan acid 0.75mg/L, sucrose
35g/L, agar 7.6g/L, IBA3.5mg/L, NAA0.075mg/L, AC0.0035%；The pH of the root media is 6.0.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the culture medium described in step (2) are：Ammonium nitrate 735mg/L,
Potassium nitrate 975mg/L, calcium chloride 183mg/L, magnesium sulfate 192.5mg/L, potassium dihydrogen phosphate 74mg/L, boric acid 15mg/L, sulfuric acid
Manganese 32.5mg/L, zinc sulfate 15mg/L, sodium molybdate 0.375mg/L, sodium selenate 0.375mg/L, copper sulphate 0.0375mg/L, sulfuric acid
Ferrous 31.4mg/L, disodium ethylene diamine tetraacetate 41.15mg/L, 471 μ g/mL of sodium selenate, glycine 3.5mg/L, Tyiamine Hd
Plain 0.75mg/L, pyridoxine hydrochloride 0.75mg/L, Yan acid 0.75mg/L, inositol 110mg/L, folic acid 0.75mg/L, biotin
0.075mg/L, sucrose 21g/L, agar 8.15g/L；The pH of the culture medium is 5.7.
The preparation method of above-mentioned culture medium is：Agar, sucrose are added to the water, heating stirring is dissolved to liquid and is translucent
Shape, it is uniform to add other material mixings, then adds distilled water to be settled to 1000mL, and pH to 5.5 is adjusted with 1mol/LNaOH solution；Institute
The preparation method of root media is stated, minimal medium is made in aforementioned manners, adds IBA, NAA, AC and stirs evenly, constant volume
To 1000mL, pH to 5.8 is adjusted with 1mol/LNaOH solution.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the nutrient solution described in step (3) are：Calcium nitrate 1161mg/
L, potassium nitrate 930mg/L, potassium dihydrogen phosphate 286mg/L, ammonium nitrate 45mg/L, magnesium sulfate 263.5mg/L, boric acid 1.35mg/L,
Manganese sulfate 0.9mg/L, zinc sulfate 0.645mg/L, copper sulphate 0.2mg/L, chelant state iron 55mg/L, ferrous sulfate 31.4mg/L,
Disodium ethylene diamine tetraacetate 41.15mg/L, 52 μ g/L of sodium selenate；The nutrient solution pH is 5.8.
Embodiment 2
A kind of implantation methods of selenium-rich water spinach of the present embodiment, mainly include the following steps that：
(1) slot device is planted：Build long 45cm, width 30cm, the planting groove of high 15cm, paving plastic film in groove, with than plantation
The smaller plastic foam plate of groove makees field planting plate, pricks hole on field planting plate, embarks on journey, into column distribution；
(2) culturing and transplanting seedlings：Water spinach tender stem is taken, is cut into small pieces after cleaning water suction, the segment of 0.2cm long is cut into after disinfection,
Breed on culture medium, keep 25 DEG C of temperature, propagation goes to and culture of rootage is carried out on root media, after 1 month after 1 month
Take out under natural lighting, indoor placement 3d, wash the culture medium on root system off, then cuttage puts field planting plate on field planting plate
In planting groove, cuttings base portion is set to be immersed in water；
(3) seedling management：Recirculating nutrient solution 2 times daily, circulates 20min, water spinach vigorous period 7d supplements one every time
Secondary moisture and nutrient solution, every batch of replacement one time of nutrition liquid, change liquid measure as 50%；
(4) prevention and control of plant diseases, pest control：1 Buddhist nun is sprayed per 7d and imitates spirit 1:1:200 times of liquid, it is 3-4 times continuous.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the water spinach kind be the big bone in Guangdong is clear, leafiness obstructs in vain,
Spire leads to one kind in dish.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the root media described in step (2) are：Potassium nitrate
1900mg/L, ammonium nitrate 1650mg/L, potassium dihydrogen phosphate 170mg/L, magnesium sulfate 370mg/L, calcium chloride 440mg/L, potassium iodide
0.83mg/L, boric acid 6.3mg/L, manganese sulfate 22.3mg/L, zinc sulfate 8.6mg/L, sodium molybdate 0.25mg/L, copper sulphate
0.025mg/L, cobalt chloride 0.025mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, ferrous sulfate 27.8mg/L, inositol
100mg/L, glycine 2mg/L, Tyiamine Hd element 0.1mg/L, puridoxine hydrochloride 0.5mg/L, Yan acid 0.5mg/L, sucrose 30g/
L, agar 7g/L, IBA2.0mg/L, NAA0.05mg/L, AC0.002%；The pH of the root media is 5.8.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the culture medium described in step (2) are：Ammonium nitrate 720mg/L,
Potassium nitrate 950mg/L, calcium chloride 166mg/L, magnesium sulfate 185mg/L, potassium dihydrogen phosphate 68mg/L, boric acid 10mg/L, manganese sulfate
25mg/L, zinc sulfate 10mg/L, sodium molybdate 0.25mg/L, sodium selenate 0.25mg/L, copper sulphate 0.025mg/L, ferrous sulfate
27.8mg/L, disodium ethylene diamine tetraacetate 37.3mg/L, 392 μ g/mL of sodium selenate, glycine 2mg/L, Tyiamine Hd element 0.5mg/
L, pyridoxine hydrochloride 0.5mg/L, Yan acid 0.5mg/L, inositol 100mg/L, folic acid 0.5mg/L, biotin 0.05mg/L, sucrose
20g/L, agar 7.8g/L；The pH of the culture medium is 5.5.
The preparation method of above-mentioned culture medium is：Agar, sucrose are added to the water, heating stirring is dissolved to liquid and is translucent
Shape, it is uniform to add other material mixings, then adds distilled water to be settled to 1000mL, and pH to 5.5 is adjusted with 1mol/LNaOH solution；Institute
The preparation method of root media is stated, minimal medium is made in aforementioned manners, adds IBA, NAA, AC and stirs evenly, constant volume
To 1000mL, pH to 5.8 is adjusted with 1mol/LNaOH solution.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the nutrient solution described in step (3) are：Calcium nitrate 1122mg/
L, potassium nitrate 910mg/L, potassium dihydrogen phosphate 272mg/L, ammonium nitrate 40mg/L, magnesium sulfate 247mg/L, boric acid 1.2mg/L, sulfuric acid
Manganese 0.8mg/L, zinc sulfate 0.09mg/L, copper sulphate 0.1mg/L, chelant state iron 4.2mg/L, ferrous sulfate 27.8mg/L, second two
Amine tetraacethyl disodium 37.3mg/L, 46 μ g/L of sodium selenate；The nutrient solution pH is 5.6.
Embodiment 3
A kind of implantation methods of selenium-rich water spinach of the present embodiment, mainly include the following steps that：
(1) slot device is planted：Build long 50cm, width 40cm, the planting groove of high 17cm, paving plastic film in groove, with than plantation
The smaller plastic foam plate of groove makees field planting plate, pricks hole on field planting plate, embarks on journey, into column distribution；
(2) culturing and transplanting seedlings：Water spinach tender stem is taken, is cut into small pieces after cleaning water suction, the segment of 0.5cm long is cut into after disinfection,
Breed on culture medium, keep 25 DEG C of temperature, propagation goes to and culture of rootage is carried out on root media, after 1 month after 1 month
Take out under natural lighting, indoor placement 3d, wash the culture medium on root system off, then cuttage puts field planting plate on field planting plate
In planting groove, cuttings base portion is set to be immersed in water；
(3) seedling management：Recirculating nutrient solution 2-3 times daily, circulates 30min, water spinach vigorous period 10d supplements every time
Moisture and nutrient solution, every batch of replacement one time of nutrition liquid, change liquid measure as 50%；
(4) prevention and control of plant diseases, pest control：1 Buddhist nun is sprayed per 7d and imitates spirit 1:1:200 times of liquid, continuous 4 times.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the water spinach kind be the big bone in Guangdong is clear, leafiness obstructs in vain,
Spire leads to one kind in dish.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the root media described in step (2) are：Potassium nitrate
2000mg/L, ammonium nitrate 1800mg/L, potassium dihydrogen phosphate 200mg/L, magnesium sulfate 400mg/L, calcium chloride 460mg/L, potassium iodide
1mg/L, boric acid 8.5mg/L, manganese sulfate 28mg/L, zinc sulfate 10mg/L, sodium molybdate 0.5mg/L, copper sulphate 0.05mg/L, chlorination
Cobalt 0.05mg/L, disodium ethylene diamine tetraacetate 40mg/L, ferrous sulfate 33mg/L, inositol 120mg/L, glycine 5mg/L, hydrochloric acid
Thiamine 0.5mg/L, puridoxine hydrochloride 1mg/L, Yan acid 1mg/L, sucrose 40g/L, agar 8.2g/L, IBA5.0mg/L,
NAA0.1mg/L, AC0.005%；The pH of the root media is 6.3.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the culture medium described in step (2) are：Ammonium nitrate 750mg/L,
Potassium nitrate 1000mg/L, calcium chloride 200mg/L, magnesium sulfate 200mg/L, potassium dihydrogen phosphate 80mg/L, boric acid 20mg/L, manganese sulfate
40mg/L, zinc sulfate 20mg/L, sodium molybdate 0.5mg/L, sodium selenate 0.5mg/L, copper sulphate 0.05mg/L, ferrous sulfate 35mg/
L, disodium ethylene diamine tetraacetate 45mg/L, 550 μ g/mL of sodium selenate, glycine 5mg/L, Tyiamine Hd element 1mg/L, hydrochloric acid pyrrole are trembled
Plain 1mg/L, Yan acid 1mg/L, inositol 120mg/L, folic acid 1mg/L, biotin 0.1mg/L, sucrose 22g/L, agar 8.5g/L；Institute
The pH for stating culture medium is 6.0.
The preparation method of above-mentioned culture medium is：Agar, sucrose are added to the water, heating stirring is dissolved to liquid and is translucent
Shape, it is uniform to add other material mixings, then adds distilled water to be settled to 1000mL, and pH to 5.5 is adjusted with 1mol/LNaOH solution；Institute
The preparation method of root media is stated, minimal medium is made in aforementioned manners, adds IBA, NAA, AC and stirs evenly, constant volume
To 1000mL, pH to 5.8 is adjusted with 1mol/LNaOH solution.
A kind of implantation methods of selenium-rich water spinach of the present embodiment, the nutrient solution described in step (3) are：Calcium nitrate 1200mg/
L, potassium nitrate 950mg/L, potassium dihydrogen phosphate 300mg/L, ammonium nitrate 50mg/L, magnesium sulfate 280mg/L, boric acid 1.5mg/L, sulfuric acid
Manganese 1mg/L, zinc sulfate 1.2mg/L, copper sulphate 0.3mg/L, chelant state iron 6.8mg/L, ferrous sulfate 35mg/L, ethylenediamine tetrem
Acid disodium 45mg/L, 58 μ g/L of sodium selenate；The nutrient solution pH is 7.0.
Comparative example 1
With embodiment 1 difference lies in when, seedling management, not comprising sodium selenate in the nutrient solution of application.
Comparative example 2
With embodiment 1 difference lies in when, seedling management, the pH of the nutrient solution of application is 5.5.
Comparative example 3
With embodiment 1 difference lies in when, seedling management, the pH of the nutrient solution of application is 7.1.
Compliance test result
Same green water cress is planted by above-described embodiment 1~3, comparative example 1~3 this six kinds of methods, plantation comes out
Effect it is as shown in the table：
As seen from the above table, three results that embodiment 1~3 is drawn have no too big difference, are planted using this method hollow
Dish, the nutritional ingredient of vegetables are improved, and selenium fertilizer can effectively improve absorption of the vegetables to element in fertilizer, improve photosynthetic work
With producing beneficial organic matter and mineral matter, planting effect is good；Embodiment is in the physics and chemistry product such as carrotene, selenium content, protein
Comparative example is better than in matter, illustrates that each component deal and step of this and adding procedure of the present invention containing selenium fertilizer cease
Manner of breathing closes；Comparative example 1 is compared with 2,3, because comparative example 2 and 3 is all to have the process containing selenium fertilizer of application, therefore in component quality
On all can be better than not adding the comparative example 3 of any selenium element fertilizer；Compared with Example 1, quality effect has difference to comparative example 2 and 3
It is different, illustrate that the pH of the nutrient solution of culture will also result in the absorption efficiency of plant pair nutritional ingredient；The present invention plants out hollow
Dish complete stool is smooth, and underground is without root tuber, and stem is hollow, leaf alternate, and ellipticity is avette or Yangtze River Delta shape.
Embodiment described above only expresses the several embodiments of the present invention, its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.
Claims (6)
1. a kind of implantation methods of selenium-rich water spinach, it is characterised in that comprise the following steps：
(1) slot device is planted：Build long 45-50cm, width 30-40cm, the planting groove of high 15-17cm, paving plastic film in groove, with than
The smaller plastic foam plate of planting groove makees field planting plate, pricks hole on field planting plate, embarks on journey, into column distribution；
(2) culturing and transplanting seedlings：Water spinach tender stem is taken, is cut into small pieces after cleaning water suction, the segment of 0.2-0.5cm long is cut into after disinfection,
Breed on culture medium, keep 25 DEG C of temperature, propagation goes to and culture of rootage is carried out on root media, after 1 month after 1 month
Take out under natural lighting, indoor placement 3d, wash the culture medium on root system off, then cuttage puts field planting plate on field planting plate
In planting groove, cuttings base portion is set to be immersed in water；
(3) seedling management：Recirculating nutrient solution 2-3 times daily, circulates 20-30min every time, and water spinach vigorous period 7-10d is mended
A moisture and nutrient solution are filled, every batch of replacement one time of nutrition liquid, changes liquid measure as 50%；
(4) prevention and control of plant diseases, pest control：1 Buddhist nun is sprayed per 7d and imitates spirit 1:1:200 times of liquid, it is 3-4 times continuous.
2. the implantation methods of a kind of selenium-rich water spinach according to claim 1, it is characterised in that the water spinach kind is
The big bone in Guangdong is clear, leafiness obstructs in vain, spire leads to one kind in dish.
A kind of 3. implantation methods of selenium-rich water spinach according to claim 1, it is characterised in that step (2) culture
Base is：Ammonium nitrate 720-750mg/L, potassium nitrate 950-1000mg/L, calcium chloride 166-200mg/L, magnesium sulfate 185-200mg/
L, potassium dihydrogen phosphate 68-80mg/L, boric acid 10-20mg/L, manganese sulfate 25-40mg/L, zinc sulfate 10-20mg/L, sodium molybdate
0.25-0.5mg/L, sodium selenate 0.25-0.5mg/L, copper sulphate 0.025-0.05mg/L, ferrous sulfate 27.8-35mg/L, second two
Amine tetraacethyl disodium 37.3-45mg/L, sodium selenate 392-550 μ g/ml, glycine 2-5mg/L, Tyiamine Hd element 0.5-1mg/L,
Pyridoxine hydrochloride 0.5-1mg/L, Yan acid 0.5-1mg/L, inositol 100-120mg/L, folic acid 0.5-1mg/L, biotin 0.05-
0.1mg/L, sucrose 20-22g/L, agar 7.8-8.5g/L；The pH of the culture medium is 5.5-6.0.
4. the implantation methods of a kind of selenium-rich water spinach according to claim 1, it is characterised in that the step (2) is taken root
Culture medium is：Potassium nitrate 1900-2000mg/L, ammonium nitrate 1650-1800mg/L, potassium dihydrogen phosphate 170-200mg/L, magnesium sulfate
370-400mg/L, calcium chloride 440-460mg/L, potassium iodide 0.83-1mg/L, boric acid 6.3-8.5mg/L, manganese sulfate 22.3-
28mg/L, zinc sulfate 8.6-10mg/L, sodium molybdate 0.25-0.5mg/L, copper sulphate 0.025-0.05mg/L, cobalt chloride 0.025-
0.05mg/L, disodium ethylene diamine tetraacetate 37.3-40mg/L, ferrous sulfate 27.8-33mg/L, inositol 100-120mg/L, sweet ammonia
Sour 2-5mg/L, Tyiamine Hd element 0.1-0.5mg/L, puridoxine hydrochloride 0.5-1mg/L, Yan acid 0.5-1mg/L, sucrose 30-40g/
L, agar 7-8.2g/L, IBA 2.0-5.0mg/L, NAA 0.05-0.1mg/L, AC 0.002-0.005%；The culture of rootage
The pH of base is 5.8-6.3.
A kind of 5. implantation methods of selenium-rich water spinach according to claim 1, it is characterised in that step (2) culture
The preparation method of base is：Agar, sucrose are added to the water, heating stirring is dissolved to liquid and is translucent shape, adds other materials
It is uniformly mixed, then adds distilled water to be settled to 1000ml, pH to 5.5 is adjusted with 1mol/L NaOH solutions；The root media
Preparation method, is made minimal medium, adds IBA, NAA, AC and stir evenly, be settled to 1000ml in aforementioned manners, uses
1mol/L NaOH solutions adjust pH to 5.8.
A kind of 6. implantation methods of selenium-rich water spinach according to claim 1, it is characterised in that step (3) nutrition
Liquid is：Calcium nitrate 1122-1200mg/L, potassium nitrate 910-950mg/L, potassium dihydrogen phosphate 272-300mg/L, ammonium nitrate 40-
50mg/L, magnesium sulfate 247-280mg/L, boric acid 1.2-1.5mg/L, manganese sulfate 0.8-1mg/L, zinc sulfate 0.09-1.2mg/L,
Copper sulphate 0.1-0.3mg/L, chelant state iron 4.2-6.8mg/L, ferrous sulfate 27.8-35mg/L, disodium ethylene diamine tetraacetate
37.3-45mg/L, sodium selenate 46-58 μ g/L；The nutrient solution pH is 5.6-7.0.
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| CN109220747A (en) * | 2018-10-22 | 2019-01-18 | 湖北文理学院 | A kind of water spinach ciltivating process |
| CN111004071A (en) * | 2019-11-26 | 2020-04-14 | 广西中农富玉国际农业科技有限公司 | Nutrient solution and water culture method for static water culture of water spinach |
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| CN115812541A (en) * | 2021-09-17 | 2023-03-21 | 中国农业科学院油料作物研究所 | Production method and application of plant source ultrahigh organic selenium |
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| CN112753513A (en) * | 2021-02-05 | 2021-05-07 | 湖北恩施中国南方马铃薯研究中心 | Water culture rapid propagation method of selenium-rich leaf vegetable type sweet potatoes |
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