CN107896973B - The selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line - Google Patents
The selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line Download PDFInfo
- Publication number
- CN107896973B CN107896973B CN201711135915.3A CN201711135915A CN107896973B CN 107896973 B CN107896973 B CN 107896973B CN 201711135915 A CN201711135915 A CN 201711135915A CN 107896973 B CN107896973 B CN 107896973B
- Authority
- CN
- China
- Prior art keywords
- plant
- fertile
- ecotope
- temperature
- single plant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A kind of selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line, comprising: 1) it with strong cold-resistant Chinese cabbage type winter rape is female parent, using the temperature sensitive sterile strain of Chinese cabbage type as male parent, hybridization；2) mutual bagging pollination between fertile plant, presses single plant after mature and carries out threshing harvest；3) in the ecotope autumn sowing that cools, Field Screening is carried out to single plant winter resistance；4) after Fertility identification, the single plant of infertility is marked, and the single plant of infertility is transplanted to High aititude cools and carry out Fertility identification behind area, fertile plant is pollinated mutually；5) single plant autumn sowing is pressed in high temperature-induced sterility ecotope and the fertile ecotope of low temperature respectively, at the florescence in next year, only the fertile strain that shows as to the strain for being accredited as infertility in high temperature-induced sterility ecotope and in the fertile ecotope of low temperature carries out isolation pollination；6) step 5) is repeated, screening obtains stable Chinese cabbage type winter rape temperature sensitive type male sterile two-purpose line.The present invention can break the production restriction bottleneck of Chinese cabbage type winter rape in practical applications.
Description
Technical field
The invention belongs to crop breeding fields, are related to a kind of sterile line breeding method more particularly to a kind of strong winter habit
The selection of Chinese cabbage type winter rape thermosensitive type male sterility line.
Background technique
Chinese cabbage type winter rape belongs to heterogeneous allometrosis, has selfing not affinity characteristic.Existing Chinese cabbage type winter rape variety
Yield potentiality is limited, and can effectively improve crop yield using male sterility.Currently, male sterile application mainly has cytoplasm
Male sterility and nuclear male sterility.However, either cytoplasmic male sterility or nuclear male sterility, both of which
It is related to three series mating, needs seed farm and breeding field.The male sterile appearance of temperature sensitive type enables three-line breeding to be reduced to two
It is breeding, is worth with certain Commercial cultivation.TwoLine Method is that system will be kept to be combined into one with sterile line, not by Rescued virus system
About, combo is free, easily acquisition influential point set.In recent years, breeder is increasingly heavier to the research and application of Bilinear hybrid rape
Depending on, improved line 6S, H90S, 373S, 533st, 417S etc. in cabbage type rape, and in turnip type rape discovery compared with
It is few, such as 29A, Guangfeng A, it yet there are no the report in relation to temperature sensitive type infertility in Chinese cabbage type winter rape.
Summary of the invention
In order to solve the above technical problems existing in the prior art, the present invention provides one kind can be in practical applications
Break the selection of the strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line of the production restriction bottleneck of Chinese cabbage type winter rape.
To achieve the goals above, the present invention adopts the following technical scheme:
A kind of selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line, it is characterised in that: the method
The following steps are included:
1) it is female parent with strong cold-resistant Chinese cabbage type winter rape, using the temperature sensitive sterile strain of Chinese cabbage type as male parent, does and hybridize, obtain miscellaneous
A kind generation, is denoted as F1 generation；
2) by the obtained F1 generation of step 1), in the ecotope that cools mid-August by combination monoseeding, into the dead leaf phase
Plastic foil cover overwintering afterwards, and the florescence in next year observes and records single plant florescence fertility state, combines mutual bagging between interior fertile plant
Pollination presses single plant after mature and carries out threshing harvest, obtains F2 for seed, the height above sea level of the ecotope that cools is not less than 2200m, temperature
Degree is not higher than 22 DEG C；
3) by the obtained F2 of step 2) for seed, in the ecotope autumn sowing that cools, Wintering Period open field soil, in winter extremely
Temperature and in subzero 20 DEG C it is below under the conditions of to single plant winter resistance carry out Field Screening, the plant that next year overwintering can turn green
As strong cold-resistant single plant；
4) the obtained strong cold-resistant single plant of step 3) is subjected to Fertility identification, the specific implementation of the Fertility identification at the florescence
Mode is: in the case where florescence field temperature is not higher than 22 DEG C, passing through the observation same day open flower stamen, anther form, flower
Medicine is withered brown flat small, and extruding sheds without pollen as sterile plant, pulls out sterile plant, retains fertile plant；And by fertile plant from base portion to
It is wiped out at upper 1/3 plant height, while removing all buds of fertile plant, the height for the stubble left is not less than 35cm；Allow the fertile plant after wiping out
Continued growth postpones the breeding time of the fertile plant after wiping out not less than 20 days until the fertile plant after wiping out is reflorescened；?
In the case that outdoor temperature is not less than 28 DEG C, Fertility identification carried out again to regeneration single plant of blooming, sterile single plant listing mark,
And the single plant of infertility is transplanted to High aititude and is cooled area, it carries out Fertility identification again after 7 days, pulls out sterile plant, by fertile plant
Pollination mutually, obtains a new generation, is denoted as F3 generation；The High aititude cool area height above sea level not less than 2200m, the High aititude cools
The temperature in area is not more than 22 DEG C；
5) it is divided to two parts to plant respectively step 4) obtained F3 generation, a copy of it presses the single plant autumn in high temperature-induced sterility ecotope
It broadcasts, the florescence in next year carries out Fertility identification to single plant, and to the strain listing mark for being accredited as infertility；Another is in the fertile life of low temperature
State area press single plant autumn sowing, the florescence in next year, only to high temperature-induced sterility ecotope be accredited as infertility strain and in the fertile ecology of low temperature
The fertile strain that shows as in area carries out isolation pollination, obtains a new generation, is denoted as F4 generation, the high temperature-induced sterility ecotope is the florescence
The temperature day highest temperature in field is not less than 28 DEG C；The fertile ecotope of low temperature is the florescence field temperature highest temperature not higher than 22 DEG C；
6) in F4 generation obtained to step 5), repeats step 5) 1-2 times, and combines Breeding objective, and screening is stablized
Chinese cabbage type winter rape temperature sensitive type male sterile two-purpose line.
The present invention has the following advantages compared with conventional method:
The present invention provides a kind of selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line, this method with
Strong cold-resistant Chinese cabbage type winter rape is maternal (♀), and the temperature sensitive sterile strain of the Chinese cabbage type of screening and identification is male parent (♂), does and hybridizes, obtains
To the first generation of hybrid (F1)；F1 generation breeding, F2 for cold hardness evaluation and Fertility identification, F3 generation and F4 for Planting in the different location, thus
Stable temperature sensitive type male sterile two-purpose line F5 generation is filtered out in hybrid generation.The invention enables the utilizations of northern Chinese cabbage type winter rape
Two line method crossbreeding is possibly realized to solve yield limitation problem.Specifically, the present invention has the advantage that
1) the environmental male sterile plants for obtaining screening and identification are cultivated into environmental sterile dual-purpose；
2) sterile dual-purpose by sterile line and keeps system to be combined into one, and need to only plant in fertile ecotope in breeding dual-purpose
System, brother and sister hand over and raise up seed, without separately plantation, therefore time and labour saving；
3) what temperature sensitive type was sterile assembles freedom, is easier to obtain influential point set；
4) recovery resource of temperature sensitive type infertility is extensive.
Specific embodiment
The present invention provides a kind of selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line, this method packets
Include following steps:
1) it is female parent with strong cold-resistant Chinese cabbage type winter rape, using the temperature sensitive sterile strain of Chinese cabbage type as male parent, does and hybridize, obtain miscellaneous
A kind generation, is denoted as F1 generation；
2) by the obtained F1 generation of step 1), in the ecotope that cools mid-August by combination monoseeding, into the dead leaf phase
Plastic foil cover overwintering afterwards, and the florescence in next year observes and records single plant florescence fertility state, combines mutual bagging between interior fertile plant
Pollination presses single plant after mature and carries out threshing harvest, obtains F2 for seed, the height above sea level for the ecotope that cools is not less than 2200m, and temperature is not
Higher than 22 DEG C；
3) by the obtained F2 of step 2) for seed, in the ecotope autumn sowing that cools, Wintering Period open field soil, in winter extremely
Temperature and in subzero 20 DEG C it is below under the conditions of to single plant winter resistance carry out Field Screening, the plant that next year overwintering can turn green
As strong cold-resistant single plant；
4) the obtained strong cold-resistant single plant of step 3) is subjected to Fertility identification, the specific embodiment of Fertility identification at the florescence
Be: in the case where florescence field temperature is not higher than 22 DEG C, by the observation same day open flower stamen, anther form, anther is withered
Brown flat small, extruding sheds without pollen as sterile plant, pulls out sterile plant, retains fertile plant；And by fertile plant from base portion upward 1/3
It is wiped out at plant height, while removing all buds of fertile plant, the height for the stubble left is not less than 35cm；The fertile plant after wiping out is allowed to continue to give birth to
It is long, until the fertile plant after wiping out is reflorescened, postpone the breeding time of the fertile plant after wiping out not less than 20 days；In outdoor temp
Degree carries out Fertility identification not less than in the case where 28 DEG C, to regeneration single plant of blooming again, sterile single plant listing mark, and will not
The single plant educated is transplanted to High aititude and cools area, carries out Fertility identification again after 7 days, pulls out sterile plant, fertile plant is awarded mutually
Powder obtains a new generation, is denoted as F3 generation；High aititude cool area height above sea level not less than 2200m, the cool temperature in area of High aititude is not more than
22℃；
5) it is divided to two parts to plant respectively step 4) obtained F3 generation, a copy of it presses the single plant autumn in high temperature-induced sterility ecotope
It broadcasts, the florescence in next year carries out Fertility identification to single plant, and to the strain listing mark for being accredited as infertility；Another is in the fertile life of low temperature
State area press single plant autumn sowing, the florescence in next year, only to high temperature-induced sterility ecotope be accredited as infertility strain and in the fertile ecology of low temperature
Area shows as fertile strain and carries out isolation pollination, obtains a new generation, is denoted as F4 generation, and high temperature-induced sterility ecotope is florescence field temperature
The highest temperature of subsisting is not less than 28 DEG C；The fertile ecotope of low temperature is the florescence field temperature highest temperature not higher than 22 DEG C；
6) in F4 generation obtained to step 5), repeats step 5) 1-2 times, and combines Breeding objective, and screening is stablized
Chinese cabbage type winter rape temperature sensitive type male sterile two-purpose line.
In the following, will in conjunction with specific embodiments, not to strong winter habit Chinese cabbage type winter rape temperature sensitive type male provided by the present invention
The selection for being is educated to be described in detail:
Embodiment 1: the breeding of temperature sensitive type dual purpose lines PK3-12S
1) the temperature sensitive sterile strain PK3-12 of the Chinese cabbage type of screening and identification in the Chinese cabbage type winter rape experimental plot of sky and water Qinzhou District
For male parentNo. 7 are maternal (♀) with strong cold-resistant Chinese cabbage type winter rape Gansu Province oil, do and hybridize, obtain the first generation of hybrid (F1 generation)；
2) by the obtained F1 generation of step 1), in Lanzhou, Qin Wangchuan (cool ecotope) is broadcast mid-August by combination simple grain
Kind, into the dead leaf phase after plastic foil cover overwintering, the florescence in next year observes and records single plant florescence fertility state, fertile in combination
Mutually bagging pollination between strain, presses single plant after mature and carries out threshing harvest, obtain F2 for seed.
3) by the obtained F2 of step 2) for seed, in Lanzhou, Qin Wangchuan (cool ecotope) autumn sowing, Wintering Period open country are got over
Winter, in winter extreme temperature and in subzero 20 DEG C it is below under the conditions of to single plant winter resistance carry out Field Screening, next year can
The overwintering plant to turn green is strong cold-resistant single plant；
4) the obtained strong cold-resistant single plant of step 3) is subjected to Fertility identification, the specific embodiment of Fertility identification at the florescence
Be: in the case where florescence field temperature is not higher than 22 DEG C, by the observation same day open flower stamen, anther form, anther is withered
Brown flat small, extruding sheds without pollen as sterile plant, pulls out sterile plant, retains fertile plant；And by fertile plant from base portion upward 1/3
It is wiped out at plant height, while removing all buds of fertile plant, the height for the stubble left is not less than 35cm；The fertile plant after wiping out is allowed to continue to give birth to
It is long, until the fertile plant after wiping out is reflorescened, postpone the breeding time of the fertile plant after wiping out not less than 20 days；In outdoor temp
Degree carries out Fertility identification not less than in the case where 28 DEG C, to regeneration single plant of blooming again, sterile single plant listing mark, and will not
The single plant educated is transplanted to Lanzhou Qin Wangchuan (High aititude cool area), carries out Fertility identification again after 7 days, pulls out sterile plant, will
Fertile plant is pollinated mutually, and a new generation is obtained, and is denoted as F3 generation；
5) in F3 generation, is divided into three parts, portion is reserved, and a autumn sowing is in sky and water Qinzhou District (high temperature-induced sterility ecotope), Yi Fenqiu
It broadcasts in Lanzhou Qin Wangchuan (the fertile ecotope of low temperature), single plant sowing, the florescence in next year carries out the single plant planted in the Qinzhou District of the sky and water
Fertility identification, to the strain listing mark for being accredited as infertility；To sky and water Qinzhou District be accredited as infertility strain and in the Lanzhou Qin
Wang Chuan shows as fertile strain and carries out bagging brother and sister friendship, records single plant florescence fertility state, and harvest time carries out single plant threshing, obtains
To F4 generation；
6) 5) step 2 time is repeated to F4 generation, therefrom selects growth potential strong, the high Chinese cabbage type winter rape of yield, as Chinese cabbage
Type winter rape thermosensitive type male sterility line PK3-12S.
Claims (1)
1. a kind of selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line, it is characterised in that: the method packet
Include following steps:
1) it is female parent with strong cold-resistant Chinese cabbage type winter rape, using the temperature sensitive sterile strain of Chinese cabbage type as male parent, does and hybridize, obtain hybrid one
In generation, is denoted as F1 generation；
2) by the obtained F1 generation of step 1), in the ecotope that cools mid-August by combination monoseeding, into the dead leaf phase after mould
Material film cover overwintering, and the florescence in next year observes and records single plant florescence fertility state, combine in mutually bagging pollination between fertile plant,
Threshing harvest is carried out by single plant after maturation, obtains F2 for seed, the height above sea level of the ecotope that cools is not less than 2200m, and temperature is not
Higher than 22 DEG C；
3) by the obtained F2 of step 2) for seed, in the ecotope autumn sowing that cools, Wintering Period open field soil, extreme temperature in winter
And in subzero 20 DEG C it is below under the conditions of Field Screening is carried out to single plant winter resistance, the plant that next year overwintering can turn green is
Strong cold-resistant single plant；
4) the obtained strong cold-resistant single plant of step 3) is subjected to Fertility identification, the specific embodiment of the Fertility identification at the florescence
Be: in the case where florescence field temperature is not higher than 22 DEG C, by the observation same day open flower stamen, anther form, anther is withered
Brown flat small, extruding sheds without pollen as sterile plant, pulls out sterile plant, retains fertile plant；And by fertile plant from base portion upward 1/3
It is wiped out at plant height, while removing all buds of fertile plant, the height for the stubble left is not less than 35cm；The fertile plant after wiping out is allowed to continue to give birth to
It is long, until the fertile plant after wiping out is reflorescened, postpone the breeding time of the fertile plant after wiping out not less than 20 days；In outdoor temp
Degree carries out Fertility identification not less than in the case where 28 DEG C, to regeneration single plant of blooming again, sterile single plant listing mark, and will not
The single plant educated is transplanted to High aititude and cools area, carries out Fertility identification again after 7 days, pulls out sterile plant, fertile plant is awarded mutually
Powder obtains a new generation, is denoted as F3 generation；The High aititude cool area height above sea level not less than 2200m, the High aititude cools the temperature in area
Degree is not more than 22 DEG C；
5) it is divided to two parts to plant respectively step 4) obtained F3 generation, a copy of it presses single plant autumn sowing in high temperature-induced sterility ecotope,
Florescence in next year carries out Fertility identification to single plant, and to the strain listing mark for being accredited as infertility；Another is in the fertile ecology of low temperature
Area press single plant autumn sowing, the florescence in next year, only to high temperature-induced sterility ecotope be accredited as infertility strain and in the fertile ecotope of low temperature
It shows as fertile strain and carries out isolation pollination, obtain a new generation, be denoted as F4 generation, the high temperature-induced sterility ecotope is florescence field
The temperature day highest temperature is not less than 28 DEG C；The fertile ecotope of low temperature is the florescence field temperature highest temperature not higher than 22 DEG C；
6) in F4 obtained to step 5) generation, repeats step 5) 1-2 time, and combines Breeding objective, screen obtain it is stable white
Dish type winter rape temperature sensitive type male sterile two-purpose line.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711135915.3A CN107896973B (en) | 2017-11-16 | 2017-11-16 | The selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711135915.3A CN107896973B (en) | 2017-11-16 | 2017-11-16 | The selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107896973A CN107896973A (en) | 2018-04-13 |
| CN107896973B true CN107896973B (en) | 2019-09-24 |
Family
ID=61845663
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711135915.3A Expired - Fee Related CN107896973B (en) | 2017-11-16 | 2017-11-16 | The selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107896973B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112931193A (en) * | 2021-04-25 | 2021-06-11 | 贵州省油料研究所(贵州省香料研究所) | Field identification method for distinguishing Moli cytoplasm sterile plants and fertile plants of rape |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2303712A1 (en) * | 1996-12-24 | 1998-07-02 | Pioneer Hi-Bred International, Inc. | Oilseed brassica containing an improved fertility restorer gene for ogura cytoplasmic male sterility |
| WO2001022805A1 (en) * | 1999-09-28 | 2001-04-05 | University Of Delhi, South Campus | Stable cytoplasmic male sterile brassica campestris plant which contain 'polima' cytoplasm and method for obtaining such plants |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101326897B (en) * | 2008-07-09 | 2011-11-09 | 西北农林科技大学 | Method for breeding celery cabbage temperature-sensitive male sterile line and temperature-insensitive male sterile line |
| CN102210263B (en) * | 2011-04-20 | 2013-04-10 | 华中农业大学 | Method for breeding rape temperature-sensitive polima cytoplasmic male sterile AB line in artificial auxiliary pollination selfing way |
| CN102884976B (en) * | 2012-08-14 | 2014-02-05 | 武汉大学 | Method for breeding hybrid rice photosensitive (thermosensitive) male sterile line |
| CN106613912A (en) * | 2016-12-23 | 2017-05-10 | 甘肃农业大学 | Breeding method of brassica napus winter rape variety with high cold resistance, high yield and good quality |
-
2017
- 2017-11-16 CN CN201711135915.3A patent/CN107896973B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2303712A1 (en) * | 1996-12-24 | 1998-07-02 | Pioneer Hi-Bred International, Inc. | Oilseed brassica containing an improved fertility restorer gene for ogura cytoplasmic male sterility |
| WO2001022805A1 (en) * | 1999-09-28 | 2001-04-05 | University Of Delhi, South Campus | Stable cytoplasmic male sterile brassica campestris plant which contain 'polima' cytoplasm and method for obtaining such plants |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107896973A (en) | 2018-04-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106386465B (en) | Method for breeding polyploid two-line hybrid rice | |
| CN109042292A (en) | A kind of hybrid rice seed breeding process of same period rice transplanting parent | |
| CN105104168A (en) | Hybrid rice breeding method using parents with close duration from seeding to heading | |
| CN107896973B (en) | The selection of strong winter habit Chinese cabbage type winter rape thermosensitive type male sterility line | |
| CN101124886B (en) | Hybrid seed production method using cotton sterile male line and recovery line to processing ratoon regeneration | |
| CN101124885B (en) | Insect pollination seed production method of cotton nuclear sterile male two-purpose lines under the condition of ratoon | |
| CN102100175A (en) | Breeding and hybrid seed-producing method of onion male sterile line | |
| CN102301946B (en) | Method for creating yellow-seeded brassica napus germplasm | |
| CN104472340A (en) | Breeding method of flax male sterility line with high stigma exertion rate and outcrossing rate | |
| CN1034848C (en) | Wild cabbage type low mustard, middle sulphur rape three-way crossbreeding technology | |
| CN102668975A (en) | Method for producing seed by using three-line hybrid rice sterile line with purple leaf marker | |
| CN105724244A (en) | Method for conducting cross breeding through homozygous parent prepared through selfing of bisexual plant and supermale | |
| CN103190336A (en) | Method for transforming wild cabbage into male sterility line of kohlrabi and breeding hybrids | |
| CN102550397B (en) | Method for cultivating dwarf stock for grafting jatropha | |
| CN103109739B (en) | Propagation method of oil sunflower Xinkui No. 14 sterile line 1666A | |
| CN101904299A (en) | Method for breeding hot pepper germ plasm resource (gene) | |
| CN101663994A (en) | Seed production method of hybridized summer-sown soybean | |
| CN101822208B (en) | Cultivation and application of hologynic South China-type cucumber South China hologynic 09 | |
| CN107593435B (en) | The identification of strong cold-resistant Chinese cabbage type winter rape study on temperature sensitive male sterility mutant and screening technique | |
| CN103229711B (en) | Breeding method of sulforaphane-rich common head cabbages | |
| CN103609434B (en) | High-yield crossbreeding method of two-line super rice parent GuiKe-2S stock seeds | |
| CN106416993A (en) | Novel seed-used summer squash breeding method | |
| CN101606484A (en) | A kind of method of utilizing colored cotton specialized character seed selection hybrid cotton | |
| CN104145806B (en) | A kind of producing method for seed of triple crossing wheat, made seed and application thereof | |
| CN103688845A (en) | Breeding method for 50% male sterile line of oat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190924 Termination date: 20201116 |