CN101663994A - Seed production method of hybridized summer-sown soybean - Google Patents
Seed production method of hybridized summer-sown soybean Download PDFInfo
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- CN101663994A CN101663994A CN200910145056A CN200910145056A CN101663994A CN 101663994 A CN101663994 A CN 101663994A CN 200910145056 A CN200910145056 A CN 200910145056A CN 200910145056 A CN200910145056 A CN 200910145056A CN 101663994 A CN101663994 A CN 101663994A
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Abstract
The invention relates to a seed production method of hybridized summer-sown soybean. Seed is produced between July and August at the average temperature of 20-25 DEG C with the rainfall below 120mm; acytoplasmic male sterile line obtained by soybean interspecific hybridization is cultivated to serve as a female parent, and the maintenance line or the restorer line of summer-sown soybean is cultivated to serve as a male parent. The method of the invention comprises the following steps: A. setting an isolation region; B. row direction selection: transmeridional row direction; C. sowing date: early sowing a sterile line on 15-25 May, and sowing the maintenance line or the restorer line planted on two sides of the sterile line by stages at the interval of 10-30 days; D. alternately planting with a narrow line and a wide line; E. thin planting; and F. parental row ratio: configuring the row ratio of the male parent to the female parent is 1:2. The seed production method of the invention can effectively improve the seed production yield by 20-30%.
Description
Technical field
The present invention relates to the producing method for seed of a kind of soybean.
Background technology
Soybean is the main source of human protein's nutrition.In recent years, 150 kilograms/mu of soybean world per unit area yields, China's per unit area yield has only about 120 kg/mu, and per unit area yield is starkly lower than world average level.Under the national conditions of having a large population and a few land at present, particularly along with the increase of population and the raising of living standards of the people, demand to soybean prod is more and more powerful, it is unpractical that simple dependence enlarged-area increases the soybean gross yield, and the new technology that must seek such as heterosis utilization improves soybean yields.Hybrid vigour is one of important channel of improving the crop yield prediction level, China paddy rice, corn are extensive use of crossbreed, made per unit area yield increase sharply 1-2 doubly, the volume increase speed of the hybrid maize of nearly more than the 20 years China's hybrid rices and the U.S., adopted the volume increase speed of conventional variety before more than 20 years, the breeding hybridized grain yield genetic progress of this explanation is bigger than conventional variety; Soybean heterosis is strong, and yield potential is big, and can solve tatoin self fixed nitrogen effectively increase production unconspicuous drawback under high fertilizer and water condition; Therefore, explore the hybrid soybean seeding technique, improve hybrid seed yield, quicken hybrid soybean and be used for producing, meaning is very great.
Soybean male is sterile to be proposed by Brim and Young (1971) the earliest; The research of soybean heterosis was just set about by the U.S. since 1975, the patent disclosure of 6 hybrid soybean seeds was just arranged from 1975 to 1996, and all because of immature in theory, operability is not strong, and can't implement for these patents, at present still low-level.China starts late in the research of soybean heterosis aspect utilizing, but makes much progress.Yu Jianzhang (1996) has applied for a patent of utilizing caryon sterile line to produce hybrid soybean seeds, because maternal row can only be isolated 1/4th sterile strain, the hybrid pick-up rate is too low, and can not remove before blooming and can educate strain, the soybean false hybrid easily takes place, and is difficult for utilizing; The academy of agricultural sciences, Jilin Province takes to this respect research from nineteen eighty-three, obtained the soya cells matter male sterile line and the homotype maintenance line of the cultivated soybean and wild soybean hybridization in 1993, realized that subsequently " three are " is supporting, and bred the first in the world hybridization spring soybean kind in 2002; The academy of agricultural sciences, Anhui Province has realized that soybean M type male sterility three line is supporting by 10 years of researches, has bred in the world first hybridization summer soybean varieties that can be directly used in production No. 1, assorted excellent beans " (Anhui beans 25) " in October, 2004; Agricultural University Of Nanjing bred cytoplasmic male sterile line and maintenance line in 1998; It is highly sterile that F1 also appears in 2 combinations of the Fuyang city institute of agricultural sciences (1995) report, the normal phenomenon of reciprocal cross fertility, and they think the genus cytoplasmic-nuclear male sterility, realize also that at present " three are " is supporting.The summer soybean crossbreed that spring soybean crossbreed that Jilin Province breeds the academy of agricultural sciences and academy of agricultural sciences, Anhui Province breed significantly is better than conventional soybean at aspects such as resistance, lodging resistance and yielding abilities, and to improving the soybean per unit area yield, the development soybean produces and is of great importance.
Though, the seed selection of hybrid soybean kind is in the ascendant, being limited to soybean is strict self pollination crop, natural outcrossing rate is low, utilize the wind pollination difficulty big, increased many difficulties for the production of hybrid seeds of hybrid soybean, have not yet to see simple and easy, a soybean cross-breeding technology efficiently, it has become the restriction hybrid soybean and has been directly used in the bottleneck that the land for growing field crops produces.
Summary of the invention
The objective of the invention is the soybean ecotope by selecting to suit, insect that screening pollination efficient is high and the cultivation management technology that matches improve hybrid seed yield rapidly, reduce the breeding cost of hybrid soybean.
Concrete technical measures of the present invention are as follows:
Production of hybrid seeds environmental selection: about 25 ℃ of mean temperature of air, the following environment that is fit to soybean production of rainfall 120mm during the selection 7-8 month soybean blossoming; As the NORTHWEST CHINA area, insect pollinator is based on yellow wild bee and black wild bee, and chrysomelid with the bigger firefly of colony's worm amount is auxiliary insect pollinator, becomes colored insect for replenishing with thrips, white butterfly etc.The cytoplasmic nuclear male sterile line that obtains with the cultivated soybean intervarietal cross is a female parent, and the maintenance line or the recovery of cultivation summer soybean are male parent;
Concrete sowing operation may further comprise the steps:
A, set up isolated area: the seed farm circumference must not be planted other soybean varieties for 200 meters, and the male parent kind should be planted in the protection zone around the seed farm, pulls out different strain according to proterties such as soybean varieties pattern, fine hair look, the ripe look of pod and pod bearing habits, the raising seed production purity.
B, row are to selection: thing capable to.In the Northwest that China's west wind blows hard, thing in the ranks air-flow connects, and is dry nice and cool, help insect flying, and the flower beanpod in the strain can be avoided the sunlight of south direct projection, is beneficial to guarantor's flower and protects pod.
C, date of seeding: male sterile line (female parent) early sowing, adopt the mode of wheat cover beans or select precocious big wheat stubble for use, sow in 15-25 day in May, because male sterile line derives from the Yellow River and Huai He River southern areas, postpone flowering stage in the Northwest's production of hybrid seeds, early sowing in good time makes soybean can bloom in the 15-20 day high temperature and little rainfall time in July in advance in production of hybrid seeds base, maintenance line or recover system's (male parent) and determine suitable sowing date with maternal flower synchronization according to the morning and evening at kind self florescence; Maintenance line or recovery system in the male sterile line planted on two sides adopt the interval to sow in 10-15 days by stages, effectively prolong the pollen supply of male parent.
D, narrow wide row plantation: narrow wide row is plantation alternately; Be narrow row line-spacing 40cm, wide every trade is apart from 60cm.The narrow row closing, be convenient to glimmering chrysomelid cover to perch with the flower thrips short distance pollinate; Spacious between wide capable strain, be convenient to shuttle flights pollination such as yellow wild bee, black wild bee, white butterfly, also be beneficial to aeration-cooling and flowering, pollen bulk storage.
E, thin planting: at medium rich water field piece, density is 10000-12000 strain/mu, fertilizer and water condition preferably field piece density be 8000-10000 strain/mu; The field envelope is controlled at the whole florescence on the departure date, is convenient to the pollination of circling in the air of honeybee, butterfly, glimmering insect such as chrysomelid.
F, father and mother's one's own profession ratio: male parent: female parent is 1: 2 row ratio configuration.
G, florescence pour water in good time: initial bloom stage keeps soil moisture content 40-60%, guarantees that abundant bulk storage of pollen and insect actively pollinate, but to the whole florescence be lower than at 70% o'clock as the signs of drought or field capacity occurring, in time pour water, protect flower and protect pod, improve hybrid seed yield.
The prevention and elimination of disease and pests of use highly toxic pesticide is prohibited in field damage by disease and insect prevention and control, duration of flowering, in order to avoid accidentally injure insect pollinator.Therefore before blooming, should conscientiously carry out the disease insect protected work of looking into, based on efficient physical (as light trap) technology prevention damage by disease and insect.
Producing method for seed of the present invention can effectively improve hybrid seed yield 20-30%.
Embodiment
Below in conjunction with embodiment, the present invention is done to describe further.
Embodiment 1
Continuous 3 years of 2006-2008 has carried out the hybrid soybean male sterile line W931A production of hybrid seeds (numerous kind) in Baoji, Shaanxi province city, NORTHWEST CHINA area, the this area belongs to the continental monsoon subhumid climate in warm temperate zone, 25.9 ℃ of 7-8 month mean temperature of air, weather is arid, and 7-8 month average precipitation is 96.6mm.
Female parent is selected cytoplasmic nuclear male sterile line W931A for use, and it is female parent that cytoplasmic nuclear male sterile line W931A comes from middle beans 20, and illiteracy 86-6 is a male parent, species hybriding, and be that the male parent continuous backcross is bred with covering 86-6; Male parent selects for use maintenance line to cover 86-6.
Concrete technical measures are: the maternal sowing 22-25 day in May, sowing 12-16 day in the June male parent first phase, sowing 25-30 day in the June second phase.The planting patterns thing is capable, and the narrow row line-spacing is 40cm to the capable alternately plantation of, width, and wide every trade is apart from being 60cm; Adopted 1 (maintenance line, male parent): 1 (male sterile line, female parent) and 1 (maintenance line, male parent): the row of 2 (male sterile line, female parents) is than configuration, and at medium rich water field piece, planting density is 10000-12000 strain/mu; Initial bloom stage keeps soil moisture content 40-60%, and the whole florescence keeps soil moisture content to be not less than 70%; The prevention and elimination of disease and pests of use highly toxic pesticide is prohibited in field damage by disease and insect prevention and control, duration of flowering, in order to avoid accidentally injure insect pollinator.Therefore before blooming, should conscientiously carry out the disease insect protected work of looking into, based on efficient physical (as light trap) technology prevention damage by disease and insect.
The result shows, adopts 1: 1 row than configuration, the average planting density 11526 strain/mus of seed farm, male sterile line 6079 strain/mus wherein, the male sterile line individual plant on average bears pods 12.3,2.2 of seeds per pods, 100-grain weight 20.1 grams are amounted to every mu and can be obtained 32.7 kilograms of sterile lines seed making output; And adopt 1: 2 row than configuration, and average planting density 11878 strain/mus, male sterile line 8209 strain/mus wherein, the male sterile line individual plant on average bears pods 11.2,2.1 of seeds per pods, 100-grain weight 20.4 restrains, and amounts to every mu can obtain 39.3 kilograms of sterile lines seed making output; The latter improves 20.18% than the former hybrid seed yield.
2006-2008 male sterile line W931A (W931A * illiteracy 86-6) hybrid seed yield table (Baoji)
Embodiment 2:2006-2008 carries out the production of hybrid seeds (crossbreed) of hybrid soybean " No. 1, assorted excellent beans " in the Baoji, climatic factor is with example 1.
Concrete technical measures are: sowing time and other condition are with example 1.Female parent is selected cytoplasmic nuclear male sterile line W931A (source is with embodiment 1) for use, and male parent is selected for use and recovered the illiteracy 98-16 of system; The planting patterns thing is capable capable to, width, planting patterns adopts 1 (recovering system, male parent): 1 (male sterile line, female parent) and 1 (recovering system, male parent): the row of 2 (male sterile line, female parents) is than configuration, at fertilizer and water condition field piece preferably, planting density is 8000-10000 strain/mu; The prevention and elimination of disease and pests of use highly toxic pesticide is prohibited in field damage by disease and insect prevention and control, duration of flowering, in order to avoid accidentally injure insect pollinator.Therefore before blooming, should conscientiously carry out the disease insect protected work of looking into, based on efficient physical (as light trap) technology prevention damage by disease and insect.
The result shows: the configuration of 1: 1 row ratio, and average planting density 10728 strain/mus (wherein male sterile line 6106 strain/mus), the male sterile line individual plant on average bears pods 12.0,2.1 of seeds per pods, 100-grain weight 20.6 grams are amounted to every mu can obtain 32.2 kilograms of crossbreed output; And the configuration of 1: 2 row ratio, average planting density 11575 strain/mus (wherein male sterile line 8120 strain/mus), the male sterile line individual plant on average bears pods 11.7,2.1 of seeds per pods, 100-grain weight 20.3 grams are amounted to every mu can obtain 41.1 kilograms of crossbreed output; The latter improves 27.64% than the former hybrid seed yield.
2006-2008 hybridization summer soybean " No. 1, assorted excellent beans " (W931A * illiteracy 98-16) hybrid seed yield table (Baoji)
Claims (3)
1, seed production method of hybridized summer-sown soybean is characterized in that: the environmental condition of the production of hybrid seeds is 20-25 ℃ of 7-8 month mean temperature of air, below the rainfall 120mm; The cytoplasmic nuclear male sterile line that obtains with the cultivated soybean intervarietal cross is a female parent, and cultivation summer soybean maintenance line or recovery are male parent;
Concrete production of hybrid seeds operation may further comprise the steps:
A, set up isolated area: the seed farm circumference must not be planted other soybean varieties for 200 meters, and the male parent kind should be planted in the protection zone around the seed farm;
B, row are to selection: thing capable to;
C, date of seeding: male sterile line early sowing, adopt the mode of wheat cover beans or select precocious big wheat stubble for use, sow in 15-25 day in May, in the maintenance line or the recovery system of male sterile line planted on two sides, sowed in 10-30 days at interval by stages;
D, narrow wide row plantation: narrow wide row is plantation alternately; The narrow row line-spacing is 40-45cm, and wide every trade distance is 60-65cm;
E, thin planting: at medium rich water field piece, planting density is 10000-12000 strain/mu; At fertilizer and water condition field piece preferably, planting density is 8000-10000 strain/mu;
F, father and mother's one's own profession ratio: male parent: female parent is 1: 2 row ratio configuration;
G, florescence pour water in good time: initial bloom stage keeps soil moisture content 40-60%, and the whole florescence keeps soil moisture content to be not less than 70%;
Field damage by disease and insect prevention and control: duration of flowering is prohibited the prevention and elimination of disease and pests of use highly toxic pesticide; Before blooming, should conscientiously carry out the disease insect protected work of looking into, based on efficient physical (as light trap) technology prevention damage by disease and insect.
2, according to the described seed production method of hybridized summer-sown soybean of claim 1, it is characterized in that: described cytoplasmic nuclear male sterile line is W931A, coming from middle beans 20 is female parent, illiteracy 86-6 is a male parent, species hybriding, and be that the male parent continuous backcross is bred with covering 86-6, filter out maintenance line simultaneously and cover 86-6; And the hybridization summer soybean recovery of carrying out the production of hybrid seeds is to cover 98-16.
3, according to the described seed production method of hybridized summer-sown soybean of claim 1, it is characterized in that: the concrete operations of by stages sowing maintenance line or recovery system in described interval 10-30 days are: be to sow every the second stage of maintenance line or recovery system more at interval in 10-15 days at interval, male sterile line both sides sowing in 10-15 days first phase maintenance lines or recovery.
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104106353A (en) * | 2013-04-26 | 2014-10-22 | 吉林省农业科学院 | Male and female parent planting method for increasing yield of hybrid soybean seeds |
CN106489720A (en) * | 2016-10-27 | 2017-03-15 | 南京农业大学 | Soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection |
CN108522195A (en) * | 2018-04-23 | 2018-09-14 | 张立君 | A kind of implantation methods improving soybean yields |
CN114223538A (en) * | 2021-12-31 | 2022-03-25 | 安徽农业大学 | Method for improving yield of soybean hybrid seed production |
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2009
- 2009-09-24 CN CN200910145056A patent/CN101663994A/en active Pending
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104106353A (en) * | 2013-04-26 | 2014-10-22 | 吉林省农业科学院 | Male and female parent planting method for increasing yield of hybrid soybean seeds |
CN104106353B (en) * | 2013-04-26 | 2016-05-18 | 吉林省农业科学院 | A kind of Parent implantation methods that improves hybrid soybean hybrid seed yield |
CN106489720A (en) * | 2016-10-27 | 2017-03-15 | 南京农业大学 | Soya bean mutually-acting nucleus male-sterile line NJCMS4A and its selection |
CN108522195A (en) * | 2018-04-23 | 2018-09-14 | 张立君 | A kind of implantation methods improving soybean yields |
CN114223538A (en) * | 2021-12-31 | 2022-03-25 | 安徽农业大学 | Method for improving yield of soybean hybrid seed production |
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Application publication date: 20100310 |