CN107840689A - The preparation method of shellfish chestnut pumpkin special nutrient fluid - Google Patents
The preparation method of shellfish chestnut pumpkin special nutrient fluid Download PDFInfo
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- CN107840689A CN107840689A CN201711272465.2A CN201711272465A CN107840689A CN 107840689 A CN107840689 A CN 107840689A CN 201711272465 A CN201711272465 A CN 201711272465A CN 107840689 A CN107840689 A CN 107840689A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F1/00—Fertilisers made from animal corpses, or parts thereof
- C05F1/005—Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, comprise the following steps:Step 1: dried peppermint leaf, cordate houttuynia, capsicum leaf are cut into fragment adds the hydrolyzate such as cellulase, enzymolysis filtering, it is standby to leave and take filtrate, then that filter residue drying is made into filter residue powder is standby;Step 2: oyster shell, Animal Bone are milled into powder and soaked with vinegar, then with deionized water dilute dilution is standby;It is Step 3: peat, filter residue powder etc. is well mixed, add dusty yeast fermentation, then the mixture after squeezing fermentation extracts zymotic fluid therein, while remaining mixture after squeezing is disperseed and carries out saponification after adding sodium hydroxide solution, saponification liquor is filtered to obtain, obtained dilution in step 2 is mixed into obtain prefabricated solution with saponification liquor;Step 4: filtrate made from step 1 mix producing with zymotic fluid made from step 3 and prefabricated solution.The present invention can improve shellfish chestnut pumpkin yield and quality.
Description
Technical field
The present invention relates to the field of planting of shellfish chestnut pumpkin.A kind of it is more particularly related to special battalion of shellfish chestnut pumpkin
The preparation method of nutrient solution.
Background technology
Shellfish chestnut pumpkin is small melon type early pumpkin new varieties, has the fast speed of growth, strong adaptability, pest and disease damage few, easy
The advantages of plantation, and because its water content is few, dry matter content is high, more resistance to transport, it is also deep to be liked by planting household, and Bei Linan
Containing abundant protein, vitamin A, vitamin B1/B2, vitamin C in melon, at the same it is rich in the pulp of every kilogram of shellfish chestnut pumpkin
Zinc containing 18g, long-term consumption can treat the illnesss such as juvenile hypoevolutism, adult's sexual hypoesthesia, skin acne, anorexia and food preference, shellfish
The content of cobalt is also to occupy first of five cereals and vegetables in the pulp of chestnut pumpkin, therefore edible shellfish chestnut pumpkin can also prevent and treat height
The illness as caused by lacking cobalt such as blood pressure, coronary heart disease, cerebrovascular disease, diabetes.But because shellfish chestnut pumpkin is newly developed comes out
Pumpkin variety, the liquid fertilizer of lack of targeted coordinate plantation, therefore are not also fully up to expectations in terms of yield, quality.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of shellfish chestnut pumpkin that can improve shellfish chestnut pumpkin yield and quality is special
With the preparation method of nutrient solution.
In order to realize according to object of the present invention and further advantage, there is provided a kind of shellfish chestnut pumpkin special nutrient fluid
Preparation method, comprise the following steps:
Step 1: by after dried peppermint leaf, cordate houttuynia, capsicum leaf washing and sterilizing, 3.5~4.5cm is cut into2Fragment be placed in quality
5~6h is soaked in the hydrochloric acid solution that fraction is 12~15%, hydrochloric acid solution is kept in immersion process at 40~50 DEG C, then will leaching
The hydrochloric acid solution that steeping has fragment is down to room temperature, then addition mass fraction is molten for 8%~10% sodium hydroxide into hydrochloric acid solution
Liquid stops up to pH value for 6.3~7.2, obtains mixed liquor, and cellulase, papain, bromelain are added into mixed liquor
Enzyme and pectin enzyme hydrolyzate, it is placed in digesting 2~3h in 38~40 DEG C of water-bath, the mixed liquor after enzymolysis is placed in reactor
In, holding pressure is 200~220kPa, and temperature is 80~90 DEG C, carry out cellulase, papain, bromelain and
The inactivation treatment of pectase, the mixed liquor after inactivation treatment is subjected to vacuum distillation concentration, pressure is kept in vacuum distillation process
For 70~80kPa, temperature is 70~80 DEG C, is filtered after continuing 40~50min, and it is standby to leave and take filtrate, then filter residue drying is made
Filter residue powder is standby;
Step 2: oyster shell, Animal Bone are milled into powder and soaked 6~10 days with vinegar, it is then dilute with deionized water
Release 500 times dilution it is standby;
Step 3: peat, filter residue powder, pig manure, cow dung, chicken manure are pressed 3.5:2:1:1:0.5 ratio is well mixed, then
Dusty yeast is added thereto and is placed in temperature as 28~38 DEG C, and humidity is to be fermented 20~30 days in 80~90% environment, then
Peat, filter residue powder, pig manure, cow dung and chicken manure mixture after squeezing fermentation extract zymotic fluid therein, while by after squeezing
Remaining peat, filter residue powder, pig manure, cow dung and chicken manure mixture disperse and add the hydroxide that mass fraction is 7~9%
It is 80~90 DEG C of progress saponifications that temperature is adjusted after sodium solution, filters to obtain saponification liquor, and the reaction time is 50~60min, then will
Obtained dilution presses 1 with saponification liquor in step 2:1 volume ratio mixes to obtain prefabricated solution;
Step 4: zymotic fluid made from filtrate made from step 1 and step 3 and prefabricated solution are pressed 3:2:1 volume ratio
Mix producing.
Preferably, it is to dry block, difference before peat, filter residue powder, pig manure, cow dung, chicken manure mixing in step 3
Particle is made with the sieve of 200~300 mesh after crushing, then is mixed.
Preferably, peat, filter residue powder, pig manure, cow dung, the mixture of chicken manure and dusty yeast press 300 in step 3:1
Mass ratio carry out mixed fermentation.
Preferably, dried peppermint leaf, cordate houttuynia, the mass ratio of capsicum leaf are 1 in step 1:0.7:1.5.
Preferably, the quality of the hydrochloric acid solution of dried peppermint leaf in step 1, cordate houttuynia, capsicum leaf total amount with soaking its fragment
Than for 1:55~60.
Preferably, dried peppermint leaf, cordate houttuynia, capsicum leaf total amount and cellulase, papain, pineapple egg in step 1
The mass ratio of white enzyme and pectin enzyme hydrolyzate is 1:22~25.
Preferably, the powder and the mass ratio of vinegar that oyster shell, Animal Bone are milled into step 2 are 1:200~250.
Preferably, remaining peat, filter residue powder, pig manure, cow dung and chicken manure mixture are with adding after being squeezed in step 3
The mass ratio of the sodium hydroxide solution entered is 1:40~50.
The present invention comprises at least following beneficial effect:
First, be prepared into nutrient solution and sprayed to shellfish chestnut pumpkin, directly absorbed by the epidermal cell of blade and stem nutrition into
Component efficiency is higher, while will not remain in soil the pollution problem for causing soil property and water quality.
Second, it can improve shellfish chestnut pumpkin yield and result from the extract compatibility in dried peppermint leaf, cordate houttuynia, capsicum leaf
Rate, shorten growth cycle, while using hydrochloric acid immersion corrosion, enzymolysis, the reservation for being evaporated under reduced pressure the means energy high degrees such as concentration
Extract activity, and dried peppermint leaf, cordate houttuynia, capsicum leaf are plant, and the extract therefrom obtained is easier by shellfish chestnut pumpkin
Absorb.
3rd, it is being obtained after vinegar soaks and easily planted rich in elements such as potassium, phosphorus in oyster shell, Animal Bone
Potassium, the phosphorus solution of thing absorption.
4th, containing abundant humic acid composition in peat, it is equipped with a certain amount of filter residue powder, pig manure, cow dung and chicken manure
Containing active ingredients such as organic fertilizer, yellow humic acid in the extract solution obtained after being jointly processed by, shellfish chestnut pumpkin can be preferably promoted to open
Flower result.
5th, by the extract in dried peppermint leaf, cordate houttuynia, capsicum leaf, the extract in oyster shell, Animal Bone, Yi Jini
Mixotrophism composition is more balanced by a certain percentage for extract in charcoal, filter residue powder, pig manure, cow dung and chicken manure mixture, effect
Fruit is more preferably.
Further advantage, target and the feature of the present invention embodies part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be noted that experimental method described in following embodiments, is conventional method unless otherwise specified, institute
Reagent and material are stated, unless otherwise specified, is commercially obtained.
<Embodiment 1>
A kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, comprises the following steps:
Step 1: by after dried peppermint leaf, cordate houttuynia, capsicum leaf washing and sterilizing, 3.5cm is cut into2Fragment be placed in mass fraction
To soak 5h in 12% hydrochloric acid solution, hydrochloric acid solution is kept in immersion process at 40 DEG C, then by immersed with the hydrochloric acid of fragment
Solution is down to room temperature, then adds into hydrochloric acid solution sodium hydroxide solution that mass fraction is 8% until pH value is 6.3 to stop, and obtains
To mixed liquor, cellulase, papain, bromelain and pectin enzyme hydrolyzate are added into mixed liquor, is placed in 38
DEG C water-bath in digest 2~3h, the mixed liquor after enzymolysis is placed in reactor, holding pressure is 200kPa, temperature 80
DEG C, the inactivation treatment of cellulase, papain, bromelain and pectase is carried out, by the mixed liquor after inactivation treatment
Vacuum distillation concentration is carried out, it is 70kPa that pressure is kept in vacuum distillation process, and temperature is 70 DEG C, filters, stays after continuing 40min
Take filtrate standby, then that filter residue powder is made is standby by filter residue drying;
Step 2: oyster shell, Animal Bone are milled into powder and soaked 6 days with vinegar, then 500 are diluted with deionized water
It is standby that dilution is obtained again;
Step 3: peat, filter residue powder, pig manure, cow dung, chicken manure are pressed 3.5:2:1:1:0.5 ratio is well mixed, then
Dusty yeast is added thereto and is placed in temperature as 28 DEG C, and humidity is to be fermented 20 days in 80% environment, then after squeezing fermentation
Peat, filter residue powder, pig manure, cow dung and chicken manure mixture extract zymotic fluid therein, at the same will remaining peat after squeezing,
Filter residue powder, pig manure, cow dung and chicken manure mixture disperse and adjust temperature after adding the sodium hydroxide solution that mass fraction is 7%
Spend for 80 DEG C of progress saponifications, filter to obtain saponification liquor, reaction time 50min, then by obtained dilution in step 2 with
Saponification liquor presses 1:1 volume ratio mixes to obtain prefabricated solution;
Step 4: zymotic fluid made from filtrate made from step 1 and step 3 and prefabricated solution are pressed 3:2:1 volume ratio
Mix producing.
In the step 3, it is to dry bulk before peat, filter residue powder, pig manure, cow dung, chicken manure mixing, passes through respectively
Cross after crushing and particle is made with the sieve of 200 mesh, then mixed.
In the step 3, peat, filter residue powder, pig manure, cow dung, the mixture of chicken manure and dusty yeast press 300:1
Mass ratio carries out mixed fermentation.
In the step 1, dried peppermint leaf, cordate houttuynia, the mass ratio of capsicum leaf are 1:0.7:1.5.
In the step 1, the mass ratio of the hydrochloric acid solution of dried peppermint leaf, cordate houttuynia, capsicum leaf total amount with soaking its fragment
For 1:55.
In the step 1, dried peppermint leaf, cordate houttuynia, capsicum leaf total amount and cellulase, papain, bromelain
The mass ratio of enzyme and pectin enzyme hydrolyzate is 1:22.
In the step 2, powder and the mass ratio of vinegar that oyster shell, Animal Bone are milled into are 1:200.
In the step 3, remaining peat, filter residue powder, pig manure, cow dung and chicken manure mixture are with adding after squeezing
Sodium hydroxide solution mass ratio be 1:40.
<Embodiment 2>
A kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, comprises the following steps:
Step 1: by after dried peppermint leaf, cordate houttuynia, capsicum leaf washing and sterilizing, 4.5cm is cut into2Fragment be placed in mass fraction
To soak 6h in 15% hydrochloric acid solution, hydrochloric acid solution is kept in immersion process at 50 DEG C, then by immersed with the hydrochloric acid of fragment
Solution is down to room temperature, then adds into hydrochloric acid solution sodium hydroxide solution that mass fraction is 10% until pH value is 7.2 to stop, and obtains
To mixed liquor, cellulase, papain, bromelain and pectin enzyme hydrolyzate are added into mixed liquor, is placed in 40
DEG C water-bath in digest 3h, the mixed liquor after enzymolysis is placed in reactor, holding pressure is 220kPa, and temperature is 90 DEG C,
The inactivation treatment of cellulase, papain, bromelain and pectase is carried out, the mixed liquor after inactivation treatment is carried out
Concentration is evaporated under reduced pressure, it is 80kPa that pressure is kept in vacuum distillation process, and temperature is 80 DEG C, is filtered after continuing 50min, leaves and takes filter
Liquid is standby, then that filter residue powder is made is standby by filter residue drying;
Step 2: oyster shell, Animal Bone are milled into powder and soaked 10 days with vinegar, then diluted with deionized water
500 times dilution it is standby;
Step 3: peat, filter residue powder, pig manure, cow dung, chicken manure are pressed 3.5:2:1:1:0.5 ratio is well mixed, then
Dusty yeast is added thereto and is placed in temperature as 38 DEG C, and humidity is to be fermented 30 days in 80~90% environment, then squeezing fermentation
Rear peat, filter residue powder, pig manure, cow dung and chicken manure mixture extract zymotic fluid therein, while will remaining mud after squeezing
Charcoal, filter residue powder, pig manure, cow dung and chicken manure mixture disperse and adjusted after adding the sodium hydroxide solution that mass fraction is 9%
Temperature is 90 DEG C of progress saponifications, filters to obtain saponification liquor, reaction time 60min, then by obtained dilution in step 2
1 is pressed with saponification liquor:1 volume ratio mixes to obtain prefabricated solution;
Step 4: zymotic fluid made from filtrate made from step 1 and step 3 and prefabricated solution are pressed 3:2:1 volume ratio
Mix producing.
In the step 3, it is to dry bulk before peat, filter residue powder, pig manure, cow dung, chicken manure mixing, passes through respectively
Cross after crushing and particle is made with the sieve of 300 mesh, then mixed.
In the step 3, peat, filter residue powder, pig manure, cow dung, the mixture of chicken manure and dusty yeast press 300:1
Mass ratio carries out mixed fermentation.
In the step 1, dried peppermint leaf, cordate houttuynia, the mass ratio of capsicum leaf are 1:0.7:1.5.
In the step 1, the mass ratio of the hydrochloric acid solution of dried peppermint leaf, cordate houttuynia, capsicum leaf total amount with soaking its fragment
For 1:60.
In the step 1, dried peppermint leaf, cordate houttuynia, capsicum leaf total amount and cellulase, papain, bromelain
The mass ratio of enzyme and pectin enzyme hydrolyzate is 1:25.
In the step 2, powder and the mass ratio of vinegar that oyster shell, Animal Bone are milled into are 1:250.
In the step 3, remaining peat, filter residue powder, pig manure, cow dung and chicken manure mixture are with adding after squeezing
Sodium hydroxide solution mass ratio be 1:50.
<Embodiment 3>
A kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, comprises the following steps:
Step 1: by after dried peppermint leaf, cordate houttuynia, capsicum leaf washing and sterilizing, 4.0cm is cut into2Fragment be placed in mass fraction
To soak 5.5h in 14% hydrochloric acid solution, hydrochloric acid solution is kept in immersion process at 45 DEG C, then by immersed with the salt of fragment
Acid solution is down to room temperature, then adds into hydrochloric acid solution sodium hydroxide solution that mass fraction is 9% until pH value is 6.8 to stop,
Mixed liquor is obtained, cellulase, papain, bromelain and pectin enzyme hydrolyzate are added into mixed liquor, is placed in
2.5h is digested in 39 DEG C of water-bath, the mixed liquor after enzymolysis is placed in reactor, holding pressure is 210kPa, temperature 85
DEG C, the inactivation treatment of cellulase, papain, bromelain and pectase is carried out, by the mixed liquor after inactivation treatment
Vacuum distillation concentration is carried out, it is 75kPa that pressure is kept in vacuum distillation process, and temperature is 75 DEG C, filters, stays after continuing 45min
Take filtrate standby, then that filter residue powder is made is standby by filter residue drying;
Step 2: oyster shell, Animal Bone are milled into powder and soaked 8 days with vinegar, then 500 are diluted with deionized water
It is standby that dilution is obtained again;
Step 3: peat, filter residue powder, pig manure, cow dung, chicken manure are pressed 3.5:2:1:1:0.5 ratio is well mixed, then
Dusty yeast is added thereto and is placed in temperature as 33 DEG C, and humidity is to be fermented 25 days in 80~90% environment, then squeezing fermentation
Rear peat, filter residue powder, pig manure, cow dung and chicken manure mixture extract zymotic fluid therein, while will remaining mud after squeezing
Charcoal, filter residue powder, pig manure, cow dung and chicken manure mixture disperse and adjusted after adding the sodium hydroxide solution that mass fraction is 8%
Temperature is 85 DEG C of progress saponifications, filters to obtain saponification liquor, reaction time 55min, then by obtained dilution in step 2
1 is pressed with saponification liquor:1 volume ratio mixes to obtain prefabricated solution;
Step 4: zymotic fluid made from filtrate made from step 1 and step 3 and prefabricated solution are pressed 3:2:1 volume ratio
Mix producing.
In the step 3, it is to dry bulk before peat, filter residue powder, pig manure, cow dung, chicken manure mixing, passes through respectively
Cross after crushing and particle is made with the sieve of 250 mesh, then mixed.
In the step 3, peat, filter residue powder, pig manure, cow dung, the mixture of chicken manure and dusty yeast press 300:1
Mass ratio carries out mixed fermentation.
In the step 1, dried peppermint leaf, cordate houttuynia, the mass ratio of capsicum leaf are 1:0.7:1.5.
In the step 1, the mass ratio of the hydrochloric acid solution of dried peppermint leaf, cordate houttuynia, capsicum leaf total amount with soaking its fragment
For 1:58.
In the step 1, dried peppermint leaf, cordate houttuynia, capsicum leaf total amount and cellulase, papain, bromelain
The mass ratio of enzyme and pectin enzyme hydrolyzate is 1:24.
In the step 2, powder and the mass ratio of vinegar that oyster shell, Animal Bone are milled into are 1:230.
In the step 3, remaining peat, filter residue powder, pig manure, cow dung and chicken manure mixture are with adding after squeezing
Sodium hydroxide solution mass ratio be 1:45.
<Comparative example 1>
A kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, implementation process is roughly the same with embodiment 3, and difference is not
Comprising step 1, filtrate and filter residue powder are free of accordingly in remaining step, other processes are constant.
<Comparative example 2>
A kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, implementation process is roughly the same with embodiment 3, and difference is not
Comprising step 3, filtrate and dilution are pressed 3 in step 4:0.5 ratio mixing, other processes are constant.
Embodiment 1, embodiment 2, embodiment 3 and nutrient solution made from comparative example 1, comparative example 2 are subjected to planting experiment, will
Each nutrient solution sprays 100 plants of shellfish chestnut pumpkin seedling, and spraying method is identical, is to spray one every 3 days after diluting 300 times
Secondary, every plant of the amount of spraying is 400ml, the gross weight of the shellfish chestnut pumpkin of each harvest is recorded after 2 months, from seedling to result
The average production cycle, the average result rate of individual plant, the results are shown in Table 1.
Table 1, the gross weight of shellfish chestnut pumpkin, growth cycle, the average result rate contrast table of individual plant
Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | |
Gross weight (kg) | 536 | 540 | 542 | 496 | 507 |
Growth cycle (d) | 24 | 25 | 23 | 30 | 39 |
Average result rate | 97.5% | 96.3% | 98.6% | 86.2% | 88.7% |
It to be not difficult to find out from table 1, the shellfish chestnut pumpkin that embodiment 1 to embodiment 3 obtains increases in yield, while from children
Seedling to the growth cycle of result also shortens, and the average result rate of individual plant also illustrates the nutrition using embodiment 1 to embodiment 3
Liquid energy improves the upgrowth situation of flower Pistil And Stamen, improves pollination success rate, reduces the situation of " colored and unreal ".
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to various suitable the field of the invention completely, can be easily for those skilled in the art
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, it is of the invention and unlimited
In specific details and shown here as the embodiment with description.
Claims (8)
1. a kind of preparation method of shellfish chestnut pumpkin special nutrient fluid, it is characterised in that comprise the following steps:
Step 1: by after dried peppermint leaf, cordate houttuynia, capsicum leaf washing and sterilizing, 3.5~4.5cm is cut into2Fragment be placed in mass fraction
Hydrochloric acid solution is kept to soak 5~6h in 12~15% hydrochloric acid solution, in immersion process at 40~50 DEG C, then will be immersed with
The hydrochloric acid solution of fragment is down to room temperature, then addition mass fraction is straight for 8%~10% sodium hydroxide solution into hydrochloric acid solution
Be 6.3~7.2 to stop to pH value, obtain mixed liquor, added into mixed liquor cellulase, papain, bromelain and
Pectin enzyme hydrolyzate, it is placed in digesting 2~3h in 38~40 DEG C of water-bath, the mixed liquor after enzymolysis is placed in reactor,
Holding pressure is 200~220kPa, and temperature is 80~90 DEG C, carries out cellulase, papain, bromelain and pectin
The inactivation treatment of enzyme, the mixed liquor after inactivation treatment is subjected to vacuum distillation concentration, it is 70 that pressure is kept in vacuum distillation process
~80kPa, temperature are 70~80 DEG C, are filtered after continuing 40~50min, and it is standby to leave and take filtrate, then filter residue drying is made into filter residue
Powder is standby;
Step 2: oyster shell, Animal Bone are milled into powder and soaked 6~10 days with vinegar, then 500 are diluted with deionized water
It is standby that dilution is obtained again;
Step 3: peat, filter residue powder, pig manure, cow dung, chicken manure are pressed 3.5:2:1:1:0.5 ratio is well mixed, then to its
Middle addition dusty yeast is placed in temperature as 28~38 DEG C, and humidity is to be fermented 20~30 days in 80~90% environment, is then squeezed
Peat, filter residue powder, pig manure, cow dung and chicken manure mixture after fermentation extract zymotic fluid therein, while will be remaining after squeezing
Peat, filter residue powder, pig manure, cow dung and chicken manure mixture disperse and add mass fraction be 7~9% sodium hydroxide it is molten
It is 80~90 DEG C of progress saponifications that temperature is adjusted after liquid, filters to obtain saponification liquor, and the reaction time is 50~60min, then by step
Obtained dilution presses 1 with saponification liquor in two:1 volume ratio mixes to obtain prefabricated solution;
Step 4: zymotic fluid made from filtrate made from step 1 and step 3 and prefabricated solution are pressed 3:2:1 volume ratio is carried out
Mix and produce.
2. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 1, it is characterised in that peat in step 3,
It is to dry bulk before filter residue powder, pig manure, cow dung, chicken manure mixing, respectively with the sieve mistake of 200~300 mesh after crushing
Sieve series obtains particle, then is mixed.
3. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 2, it is characterised in that peat in step 3,
Filter residue powder, pig manure, cow dung, the mixture of chicken manure and dusty yeast press 300:1 mass ratio carries out mixed fermentation.
4. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 1, it is characterised in that peppermint in step 1
Leaf, cordate houttuynia, the mass ratio of capsicum leaf are 1:0.7:1.5.
5. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 1, it is characterised in that peppermint in step 1
The mass ratio of the hydrochloric acid solution of leaf, cordate houttuynia, capsicum leaf total amount with soaking its fragment is 1:55~60.
6. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 1, it is characterised in that peppermint in step 1
Leaf, cordate houttuynia, capsicum leaf total amount and the mass ratio of cellulase, papain, bromelain and pectin enzyme hydrolyzate are
1:22~25.
7. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 1, it is characterised in that oyster in step 2
The powder and the mass ratio of vinegar that shell, Animal Bone are milled into are 1:200~250.
8. the preparation method of shellfish chestnut pumpkin special nutrient fluid as claimed in claim 1, it is characterised in that after being squeezed in step 3
The mass ratio of the sodium hydroxide solution of remaining peat, filter residue powder, pig manure, cow dung and chicken manure mixture with adding is 1:40
~50.
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