CN107836466A - A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof - Google Patents
A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof Download PDFInfo
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- CN107836466A CN107836466A CN201711103968.7A CN201711103968A CN107836466A CN 107836466 A CN107836466 A CN 107836466A CN 201711103968 A CN201711103968 A CN 201711103968A CN 107836466 A CN107836466 A CN 107836466A
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- parts
- tea tree
- ethereal oil
- tree ethereal
- animals
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/28—Myrtaceae [Myrtle family], e.g. teatree or clove
Abstract
The invention discloses a kind of natural disinfectant agent for animals of plant source, include following component：3 12 parts of tea tree ethereal oil, 5 20 parts of Tween 80,40 5 20 parts of PEG, 10 30 parts of ethanol, 20 80 parts of deionized water, multiclass animal, including the disinfection of the body surface of livestock and poultry, aquatic biological and pet etc., environment, medical space and apparatus, process units and pipeline and animal colony house can be properly applied to, has widened application of the plants essential oil in disinfectant for animals.The disinfectant has significant microorganism killing to act on, and has the characteristics that short sterilizing time, sterilization of high efficiency, stability are good, has no toxic side effect.
Description
Technical field
The present invention relates to Chemistry for Chinese Traditional Medicine and chemistry of micro-organisms field, more particularly to a kind of natural disinfectant agent for animals of tea tree ethereal oil
And preparation method thereof.
Background technology
Bacterium can cause a large amount of diseases of livestock and poultry and pet, and the thimerosal used at present is mostly chemical products, although
Can be with effective sterilizing, but the health of the production and pet on livestock and poultry has certain influence.With animal husbandry and pet industry
Fast development, also increasingly payes attention to how effectively sterilizing and not for the disease people of bacterial pet and livestock and poultry
The upgrowth situation of animal is influenceed, becomes an important research direction.For example, bird flu can cause extremely to people and poultry simultaneously
Threat is died, its morbidity and mortality is high, and the chicken group of infection usually " is annihilated ", and being set to Class A by International Office of Epizootics infects
Disease.Majority causes death with a variety of organ failures such as serious pneumonia, severe patient's heart, kidney after human infection, and case fatality rate is very high.
And pathogenic microorganisms can be propagated by number of ways such as alimentary canal, respiratory tract, skin injury and eye conjunctivas.In addition, such as milk cow
The sterilization of body surface breast and conveyance conduit of milking, it cannot get sufficiently sterilised or have chemical residues, can all cause dairy products
Quality decline, it is same the problem of also often cause food security and health major reason.
The disinfectant sold at present on domestic and international market is mainly chemical classes disinfectant, but usually produces drug resistance and medicine
The problems such as residual, it is impossible to convenient use, or even because of medicament residue and caused by more economic losses.
Australia tea tree also known as narrow leaved tea tree, it is Myrtaceae paperbark, its tea tree ethereal oil (TTO) has wide spectrum
Antibacterium and antifungal activity, and its persister to many bacteriums and fungi also show that well suppress and kill make
With.Tea tree ethereal oil has been developed into polytype product such as preservative, antiseptic and aromatic, being mainly used in abroad
The numerous areas such as cosmetic, medicine, food flavor and beauty industry.China is not filled also to the antibacterial action and mechanism of tea tree ethereal oil
The understanding divided so that the research and development of its corresponding product relatively lag behind, even if also there is certain exploitation on product, but still are only confined to
Perfumed soap, bath foam, conditioning liquid, toothpaste, mildy wash etc., in pharmaceuticals industry, especially veterinary drug industry, do not formed really also
Tea tree ethereal oil product market.
The Main Ingredients and Appearance of tea tree ethereal oil is pine tar -4- alcohol and 1-8 cineoles, will not stimulate skin and eyes, it is nontoxic simultaneously
It is biodegradable.Tea tree ethereal oil disinfectant of the present invention is a kind of environment-friendly disinfectant, fast degradation and pollution-free be ensure that
It is environmentally safe, it is pollution-free to underground water.
The content of the invention
The technical problems to be solved by the invention are to overcome the deficiencies in the prior art, there is provided when one kind has sterilization
Between short, sterilization of high efficiency, stability is good, the tea tree ethereal oil natural disinfectant agent for animals for the features such as having no toxic side effect and its preparation side
Method, suitable prolonged application in multiclass animal (including livestock and poultry, aquatic biological and pet etc.) body surface, environment, medical space and apparatus,
Disinfection of process units and pipeline and animal colony house etc. etc..
The present invention is to be achieved by the following technical programs：
A kind of tea tree ethereal oil natural disinfectant agent for animals, it contains following component by its volume parts ratio：Tea tree ethereal oil 3-12
Part, Tween-80 5-20 parts, PEG-40 5-20 parts, ethanol 10-30 parts, deionized water 20-80 parts.
The tea tree ethereal oil is that 4- pine tars alcohol content is more than 30%, and 1,8 cineole content is less than 5%.
The volume parts ratio is：5 parts of tea tree ethereal oil, 10 parts of Tween-80,10 parts of PEG-40,15 parts of ethanol, water 60
Part.
A kind of preparation method of tea tree ethereal oil natural disinfectant agent for animals, comprises the following steps：
(1) each component is weighed in parts by weight：Tea tree ethereal oil 3-12 parts, Tween-80 5-20 parts, PEG-40 5-20 parts,
Ethanol 10-30 parts, deionized water 20-80 parts.
(2) tea tree ethereal oil, Tween-80, the PEG-40 that are weighed in step (1) are heated to 35-37 DEG C, mixed, carried out
Stirring, makes it fully dissolve；
(3) into step (2), gained mixed liquor is cooled to less than 25 DEG C after stirring, and adds in step (1) and is weighed
Ethanol and deionized water, stir and evenly mix and produce.
The beneficial effects of the invention are as follows：
This disinfectant has to common pathogens such as staphylococcus aureus, Escherichia coli and Candida albicans significantly to kill
Bacterium effect and stability, the plants essential oil disinfectant belong to actual nontoxic level material, and preparation method is simple, is easy to industrialize, and fits
Preferably it is applied to multiclass animal, including the body surface of livestock and poultry, aquatic biological and pet etc., environment, medical space and apparatus, process units
With pipeline and the disinfection of animal colony house.
Embodiment
In order that those skilled in the art more fully understand technical scheme, implement with reference to optimal
The present invention is described in further detail for example.
Embodiment 1
Its component and its mass fraction are：
3 parts of tea tree ethereal oil, 5 parts of Tween-80,5 parts of PEG-40,10 parts of ethanol, 20 parts of deionized water.
Its preparation method is as follows：
(1) each component is weighed in parts by weight：3 parts of tea tree ethereal oil, 5 parts of Tween-80, PEG-405 parts, 10 parts of ethanol, go
20 parts of ionized water；
(2) tea tree ethereal oil, Tween-80, the PEG-40 weighed into step (1) is heated to 35-37 DEG C, mixes, and carries out
Stirring, makes it fully dissolve；
(3) into step (2), gained mixed liquor is cooled to less than 25 DEG C after stirring, and adds in step (1) and is weighed
Ethanol and deionized water, stir and evenly mix and produce.
Embodiment 2
Its component and its mass fraction are：
12 parts of tea tree ethereal oil, 20 parts of Tween-80,20 parts of PEG-40,30 parts of ethanol, 80 parts of deionized water.
Its preparation method is as follows：
(1) each component is weighed in parts by weight：12 parts of tea tree ethereal oil, 20 parts of Tween-80,20 parts of PEG-40, ethanol 30
Part, 80 parts of deionized water；
(2) tea tree ethereal oil, Tween-80, the PEG-40 weighed into step (1) is heated to 35-37 DEG C, mixes, and carries out
Stirring, makes it fully dissolve；
(3) into step (2), gained mixed liquor is cooled to less than 25 DEG C after stirring, and adds in step (1) and is weighed
Ethanol and deionized water, stir and evenly mix and produce.
Embodiment 3
Its component and its mass fraction are：
5 parts of tea tree ethereal oil, 10 parts of Tween-80,10 parts of PEG-40,15 parts of ethanol, 60 parts of deionized water.
Its preparation method is as follows：
(1) each component is weighed in parts by weight：5 parts of tea tree ethereal oil, 10 parts of Tween-80,10 parts of PEG-40, ethanol 15
Part, 60 parts of water；
(2) tea tree ethereal oil, Tween-80, the PEG-40 weighed into step (1) is heated to 35-37 DEG C, mixes, and carries out
Stirring, makes it fully dissolve；
(3) into step (2), gained mixed liquor is cooled to less than 25 DEG C after stirring, and adds in step (1) and is weighed
Ethanol and deionized water, stir and evenly mix and produce.
Germicidal test is carried out to the tea tree ethereal oil disinfectant for animals in above-described embodiment, step is as follows：
(1) the tea tree ethereal oil disinfectant for animals being respectively adopted in above-described embodiment 1-3 prepares 3%, 5%, 12% tea tree
Essential oil disinfection liquid.
(2) preparation experiment bacteria suspension, concentration are 1 × 107Cfu/ml~5 × 107cfu/ml。
(3) the sterile Boiling tube of sterilizing test is taken, 0.5ml experiment bacteria suspensions is first added, adds the organic interference of 0.5ml
Material, mix, put in 20 DEG C of ± 1 DEG C of water-baths after 5min, above-mentioned concentration thimerosal 4.0ml injections are drawn wherein with aseptic straw,
It is rapid to mix and clock immediately.
(4) bacterium to be tested interacts to each scheduled time, Escherichia coli, the thimerosal of Pseudomonas aeruginosa 2% with disinfectant
20min is acted on, staphylococcus aureus 3.5% acts on 60min, the thimerosal effect 60min of Candida albicans 5%, inhaled respectively
Take 0.5ml test organisms to be added on disinfectant mixed liquor in the sterilized nertralizers of 4.5ml, mix.
(5) each pipe test organisms is drawn 1.0ml sample liquids, pressed respectively with disinfectant mixed liquor after adding nertralizer effect 10min
Viable bacteria culture method of counting measure survival bacterium number, 2 plates of often pipe sample liquid inoculation.As the clump count grown on flat board it is more when,
After 10 times of dilutions of series can be carried out, then carry out viable bacteria culture and count.
(6) thimerosal is replaced with PBS dilutions, parallel test is carried out, as positive controls.
(7) Ai Jiajie plant sterilization sprays are used, are produced by Beijing PuRuiHuaKang Biology Science and Technology Co., Ltd., as medicine pair
According to group.
(8) bacteria test sample standard deviation is cultivated in 37 DEG C of incubators, and final result is observed to bacterial propagule culture 48h；It is right
Candida albicans need to cultivate 72h, observe final result.
(9) experiment is repeated 3 times, and calculates the viable bacteria concentration (cfu/ml) of each group, calculates sterilizing rate.
The concrete outcome of Suspension quantitative bactericidal test is as shown in table 1：
Table 1
Described above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (4)
1. a kind of tea tree ethereal oil natural disinfectant agent for animals, it is characterised in that it contains following component by its volume parts ratio：Tea tree
Essential oil 3-12 parts, Tween-80 5-20 parts, PEG-40 5-20 parts, ethanol 10-30 parts, deionized water 20-80 parts.
2. a kind of tea tree ethereal oil natural disinfectant agent for animals according to claim 1, it is characterised in that the tea tree ethereal oil is
4- pine tars alcohol content is more than 30%, and 1,8 cineole content is less than 5%.
A kind of 3. tea tree ethereal oil natural disinfectant agent for animals according to claim 1, it is characterised in that the volume parts ratio
For：5 parts of tea tree ethereal oil, 10 parts of Tween-80,10 parts of PEG-40,15 parts of ethanol, 60 parts of water.
A kind of 4. preparation method of tea tree ethereal oil natural disinfectant agent for animals according to claim 1, it is characterised in that including
Following steps：
(1) each component is weighed in parts by weight：Tea tree ethereal oil 3-12 parts, Tween-80 5-20 parts, PEG-40 5-20 parts, ethanol
10-30 parts, deionized water 20-80 parts.
(2) tea tree ethereal oil, Tween-80, the PEG-40 that are weighed in step (1) are heated to 35-37 DEG C, mixing, are stirred,
It is set fully to dissolve；
(3) into step (2), gained mixed liquor is cooled to less than 25 DEG C after stirring, and adds in step (1) second weighed
Alcohol and deionized water, stir and evenly mix and produce.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711103968.7A CN107836466A (en) | 2017-11-10 | 2017-11-10 | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711103968.7A CN107836466A (en) | 2017-11-10 | 2017-11-10 | A kind of tea tree ethereal oil natural disinfectant agent for animals and preparation method thereof |
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| CN107836466A true CN107836466A (en) | 2018-03-27 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109805038A (en) * | 2019-03-05 | 2019-05-28 | 天津中澳嘉喜诺生物科技有限公司 | A kind of Australia tea tree essential oil disinfection agent and its preparation method and application |
| CN110583702A (en) * | 2019-09-18 | 2019-12-20 | 禹州市天源生物科技有限公司 | Preparation method of tea tree essential oil disinfectant for animals |
| CN111214398A (en) * | 2020-03-19 | 2020-06-02 | 潍坊富邦药业有限公司 | Clear and transparent water-soluble tea tree essential oil and preparation method thereof |
| CN113144154A (en) * | 2021-03-06 | 2021-07-23 | 潍坊诺达药业有限公司 | Composite traditional Chinese medicine essential oil for cold dispelling and cough relieving of livestock |
| CN116440015A (en) * | 2023-02-13 | 2023-07-18 | 大德(浙江)生物科技有限公司 | Plant component disinfectant and self-provided process thereof |
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| CN103385926A (en) * | 2013-07-09 | 2013-11-13 | 武汉烺祺生物科技有限公司 | Antibiosis gel for curing cervical and vaginal diseases of gynaecology |
| CN104224923A (en) * | 2014-07-07 | 2014-12-24 | 海南华拓天涯制药有限公司 | Tea tree essential oil antimicrobial gel for treating vaginal disease in gynecology |
| CN106973892A (en) * | 2017-05-10 | 2017-07-25 | 天津中澳嘉喜诺生物科技有限公司 | A kind of plants essential oil disinfectant for public place |
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2017
- 2017-11-10 CN CN201711103968.7A patent/CN107836466A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103385926A (en) * | 2013-07-09 | 2013-11-13 | 武汉烺祺生物科技有限公司 | Antibiosis gel for curing cervical and vaginal diseases of gynaecology |
| CN104224923A (en) * | 2014-07-07 | 2014-12-24 | 海南华拓天涯制药有限公司 | Tea tree essential oil antimicrobial gel for treating vaginal disease in gynecology |
| CN106973892A (en) * | 2017-05-10 | 2017-07-25 | 天津中澳嘉喜诺生物科技有限公司 | A kind of plants essential oil disinfectant for public place |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109805038A (en) * | 2019-03-05 | 2019-05-28 | 天津中澳嘉喜诺生物科技有限公司 | A kind of Australia tea tree essential oil disinfection agent and its preparation method and application |
| CN110583702A (en) * | 2019-09-18 | 2019-12-20 | 禹州市天源生物科技有限公司 | Preparation method of tea tree essential oil disinfectant for animals |
| CN111214398A (en) * | 2020-03-19 | 2020-06-02 | 潍坊富邦药业有限公司 | Clear and transparent water-soluble tea tree essential oil and preparation method thereof |
| CN113144154A (en) * | 2021-03-06 | 2021-07-23 | 潍坊诺达药业有限公司 | Composite traditional Chinese medicine essential oil for cold dispelling and cough relieving of livestock |
| CN113144154B (en) * | 2021-03-06 | 2022-03-01 | 潍坊诺达药业有限公司 | Composite traditional Chinese medicine essential oil for cold dispelling and cough relieving of livestock |
| CN116440015A (en) * | 2023-02-13 | 2023-07-18 | 大德(浙江)生物科技有限公司 | Plant component disinfectant and self-provided process thereof |
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Application publication date: 20180327 |