CN107801628A - A kind of Chinese cabbage parent greenhouse adds for mating system - Google Patents
A kind of Chinese cabbage parent greenhouse adds for mating system Download PDFInfo
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- CN107801628A CN107801628A CN201711017653.0A CN201711017653A CN107801628A CN 107801628 A CN107801628 A CN 107801628A CN 201711017653 A CN201711017653 A CN 201711017653A CN 107801628 A CN107801628 A CN 107801628A
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- chinese cabbage
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- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 title claims abstract description 31
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 title claims abstract description 31
- 230000013011 mating Effects 0.000 title claims abstract description 16
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 title claims abstract 13
- 238000009395 breeding Methods 0.000 claims abstract description 10
- 230000001488 breeding effect Effects 0.000 claims abstract description 10
- 238000003306 harvesting Methods 0.000 claims abstract description 10
- 230000010152 pollination Effects 0.000 claims abstract description 8
- 238000009331 sowing Methods 0.000 claims abstract description 5
- 239000003337 fertilizer Substances 0.000 claims description 15
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
- 239000010455 vermiculite Substances 0.000 claims description 8
- 229910052902 vermiculite Inorganic materials 0.000 claims description 8
- 235000019354 vermiculite Nutrition 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000001963 growth medium Substances 0.000 claims description 6
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 4
- 229910052796 boron Inorganic materials 0.000 claims description 4
- 210000003608 fece Anatomy 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 239000010871 livestock manure Substances 0.000 claims description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 4
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 239000011591 potassium Substances 0.000 claims description 4
- 229910052700 potassium Inorganic materials 0.000 claims description 4
- 239000007921 spray Substances 0.000 claims description 4
- 230000008961 swelling Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen(.) Chemical compound [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000001850 reproductive effect Effects 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 244000221633 Brassica rapa subsp chinensis Species 0.000 description 19
- 238000000034 method Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 241001124076 Aphididae Species 0.000 description 2
- 241001232787 Epiphragma Species 0.000 description 2
- 241000233679 Peronosporaceae Species 0.000 description 2
- 241000255969 Pieris brassicae Species 0.000 description 2
- 208000036142 Viral infection Diseases 0.000 description 2
- 230000002631 hypothermal effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000027272 reproductive process Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
- A01H3/02—Processes for modifying phenotypes, e.g. symbiosis with bacteria by controlling duration, wavelength, intensity, or periodicity of illumination
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention relates to Chinese cabbage plus for raising technology field, and in particular to a kind of Chinese cabbage parent greenhouse adds for mating system, including following breeds step：By the first generation in late December sowing with greenhouse, vernalization is carried out under the conditions of greenhouse natural low temperature；It is colonized in open country net canopy mid-March and carries out artificial pollination, harvests parental seed mid-June；Seed greenhouse is soaked seed at the beginning of 7 months, 11~12h of vernalization；Vernalization treatment is carried out at low ambient temperatures, and August is sowed seed on 1st~5, and 5~6 true leaves are colonized in greenhouse by August on the 15th~20, artificial cabage-type rape after bolting；Seed maturity harvests by the end of November.The mating system of the present invention, utilize the characteristic of Chinese cabbage seed low temperature vernalization, under conditions of its natural growth-development law is not changed, artificial manufacture cryogenic conditions, Chinese cabbage optimum growh developing environment is created with reference to greenhouse, realize 1 year and blossom and have seeds twice, increase reproductive order of generation, accelerate Chinese cabbage breed breeding speed.
Description
Technical field
The present invention relates to Chinese cabbage plus for raising technology field, and in particular to a kind of Chinese cabbage parent greenhouse adds generation to breed
Method.
Background technology
Chinese cabbage (Brassica campestris ssp.chinensis Makino) originates in China, is southerner
The most popular staple vegetable of people's eating throughout the year, is gradually expanded to the state such as northern area, Japan, the U.S., Southeast Asia also in recent years
Introducing and planting.Chinese cabbage is biennial plant, and being transferred to reproductive growth by nutrient growth needs low temperature vernalization and long-day to induce,
In breeding process, the screening and innovation of material need to pass through the breeding links such as backcrossing, selfing and hybridization, typically be selfed for 5~6 generations
Homozygous breeding material can be obtained.A generation can only be bred within 1 year under field conditions (factors), so as to have impact on the process of breeding work, be
Accelerate Chinese cabbage breed breeding speed, it is necessary to which carrying out adding generation, (adding generation is 1 year under conditions of manual control
Breed more generations).Add therefore, the present invention provides a kind of time short Chinese cabbage parent greenhouse of breeding for mating system.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of Chinese cabbage parent greenhouse to add generation to breed
Method, it is the characteristic of seed low temperature vernalization using Chinese cabbage, under conditions of its natural growth-development law is not changed, profit
With equipment such as refrigerator, incubators, cryogenic conditions are artificially manufactured, Chinese cabbage seed is handled by artificial hypothermia, with reference to temperature
Chinese cabbage optimum growh developing environment is created in room, realizes 1 year and blossoms and has seeds twice, increases reproductive order of generation.
The purpose of the present invention is achieved through the following technical solutions：
A kind of Chinese cabbage parent greenhouse adds for mating system, including following breeds step：
S1. by the first generation in the sowing of First Year late December and greenhouse, using 1:1 garden mould and vermiculite is as culture
Base, 18~22 DEG C of daytime in greenhouse, vernalization is carried out under the conditions of 3~8 DEG C of night；
S2. it is colonized in open country net canopy mid-March in Second Year, bolting is bloomed between Second Year 18 to April 22 April,
Then artificial pollination is carried out, terminates pollination after one month；
S3. in Second Year harvest mid-June parental seed；
S4. at the beginning of Second Year 7 months, seed soaking 4~4.5h in parental seed greenhouse after the abundant water swelling of seed, will be planted
Son is positioned in the culture dish for being covered with double-layer filter paper, adds water to be sufficiently humidified so as to by filter paper, is placed in 20~25 DEG C of illumination boxs and is urged
11~12h of bud；
S5. after Seed sprouting, seed is placed under 0~5 DEG C of environment and carries out vernalization treatment 30~35 days；
S6. after vernalization terminates, seed was seeded into Second Year August to August in greenhouse in 5 within 1st, using 1:1 garden
Soil and vermiculite are as culture medium；
S7. 5~6 true leaves were colonized in Second Year August to August in greenhouse in 20 within 15th, in Second Year September 5 to September
Bolting between 10 days, start artificial cabage-type rape；
S8. Second Year early November be capped film to improve warm indoor temperature, Second Year by the end of November seed fully into
It is ripe, harvest.
Further, in the step S2 and S7, base fertilizer is used sufficient before field planting, per 66.7m2Apply high-quality farm manure
300kg, NPK 5kg, 0.1~0.2kg of boron fertilizer.
Further, chase after an instant nitrogenousfertilizer within 7~10 days after field planting and carry seedling.
Further, after bolting and before full blossom, per 66.7m2Nitrogen, phosphorus, potassium 1~1.5kg of NPK are chased after, applies 1~2
It is secondary, spray foliar fertilizer extra-nutrition.
Further, the foliar fertilizer is 0.3% potassium dihydrogen phosphate.
Further, the hole tray that 32 or 50 holes are respectively adopted is used to sow the first generation and parental seed.
Further, disease control is periodically carried out in reproductive process.
The beneficial effects of the invention are as follows：Chinese cabbage parent greenhouse of the present invention adds for mating system, white using not balling
Dish is the characteristic of seed low temperature vernalization, under conditions of its natural growth-development law is not changed, artificially manufactures cryogenic conditions, makes
Chinese cabbage seed is handled by artificial hypothermia, is created Chinese cabbage optimum growh developing environment with reference to greenhouse, is realized one
Year blossoms and has seeds twice, increases reproductive order of generation, accelerates Chinese cabbage breed breeding speed.
Embodiment
Technical scheme is described in further detail with reference to specific embodiment, but protection scope of the present invention is not
It is confined to as described below.
Embodiment
A kind of Chinese cabbage parent greenhouse adds for mating system, including following breeds step：
S1. by the first generation in the sowing of First Year late December and greenhouse, using 1:1 garden mould and vermiculite is as culture
Base, 18~22 DEG C of daytime in greenhouse, vernalization is carried out under the conditions of 3~8 DEG C of night；
S2. it is colonized in open country net canopy mid-March in Second Year, bolting is bloomed between Second Year 18 to April 22 April,
Then artificial pollination is carried out, terminates pollination after one month；
S3. in Second Year harvest mid-June parental seed；
S4. at the beginning of Second Year 7 months, seed soaking 4~4.5h in parental seed greenhouse after the abundant water swelling of seed, will be planted
Son is positioned in the culture dish for being covered with double-layer filter paper, adds water to be sufficiently humidified so as to by filter paper, is placed in 20~25 DEG C of illumination boxs and is urged
11~12h of bud；
S5. after Seed sprouting, seed is placed under 0~5 DEG C of environment and carries out vernalization treatment 30~35 days；
S6. after vernalization terminates, seed was seeded into Second Year August to August in greenhouse in 5 within 1st, using 1:1 garden
Soil and vermiculite are as culture medium；
S7. 5~6 true leaves were colonized in Second Year August to August in greenhouse in 20 within 15th, in Second Year September 5 to September
Bolting between 10 days, start artificial cabage-type rape；
S8. Second Year early November be capped film to improve warm indoor temperature, Second Year by the end of November seed fully into
It is ripe, harvest.
In a preferred embodiment, in the step S2 and S7, base fertilizer is used sufficient before field planting, per 66.7m2Apply high-quality
Farm manure 300kg, NPK 5kg, 0.1~0.2kg of boron fertilizer.
In a preferred embodiment, chase after an instant nitrogenousfertilizer within 7~10 days after field planting and carry seedling, florescence should not chase after nitrogen again
Fertilizer, in order to avoid the remaining green when it is due to become yellow and ripe delay seed maturity of plant.
In a preferred embodiment, after bolting and before full blossom, per 66.7m2Chase after nitrogen, phosphorus, potassium NPK 1~
1.5kg, apply 1~2 time, spray foliar fertilizer extra-nutrition.
In a preferred embodiment, the foliar fertilizer is 0.3% potassium dihydrogen phosphate.
In a preferred embodiment, the hole tray that 32 or 50 holes are respectively adopted is used to sow the first generation and parental seed.
In a preferred embodiment, disease control is periodically carried out in reproductive process.It is high in canopy after warmhouse booth epiphragma
Warm high humidity, aphid, cabbage caterpillar, virosis, downy mildew, soft rot etc. easily occurs, need to prevent and treat in time.
Test example
Carried out using the present invention's plus for mating system in Shijiazhuang District plus generation breeds, concrete operation method is：First
Late December generation is sowed, and the hole tray for being seeded into 32 or 50 holes is positioned over greenhouse, with 1:1 garden mould and vermiculite makees culture medium, profit
Vernalization is carried out with greenhouse natural low temperature condition, is colonized in open country net canopy mid-March, on April 20 or so, bolting was bloomed, and was carried out artificial
Cabage-type rape, on May 20 or so, pollination terminated, and mid-June harvests seed, and the yield and plumpness of parental seed are fine；Second
At the beginning of 7 months from generation to generation, the seed room temperature of harvest is soaked seed 4~4.5h, after the abundant water swelling of seed, seed is positioned over be covered with it is double
In the culture dish of layer filter paper, add a small amount of water to be sufficiently humidified so as to by filter paper, be placed in vernalization 11-12h in 20~25 DEG C of illumination boxs；Treat
After Seed sprouting, seed is placed in progress 30~35d of vernalization treatment in 0~5 DEG C of refrigerator, after vernalization terminates, August 1~5 day will
The hole tray that seed is seeded into 32 or 50 holes is positioned over greenhouse, with 1:1 garden mould and vermiculite makees culture medium, August 15~20 days 5~6
Piece true leaf is colonized in greenhouse, and September bolting on the 5th~10 starts artificial cabage-type rape, and early November is capped film to improve greenhouse
Interior temperature, ensure to blossom and bear fruit is normally carried out；Seed is fully ripe by the end of November, harvest.
During test example progress, after Chinese cabbage seed vernalization processing, carry out adding generation in Shijiazhuang District autumn
Breeding is feasible, it is important to selects appropriate vernalization number of days and sowing time；Warmhouse booth late fall culture underglass, it more can guarantee that and add
What foundry was made is smoothed out；In terms of the cultivation technique for adding generation, base fertilizer is used sufficient before field planting, per 66.7m2Apply high-quality farm manure
300kg, NPK 5kg, 0.1~0.2kg of boron fertilizer, chase after within 7-10 days after field planting an instant nitrogenousfertilizer and carry seedling, florescence is unsuitable
Nitrogen dressing again, in order to avoid the remaining green when it is due to become yellow and ripe delay seed maturity of plant；If soil fertility is poor, after bolting and before full blossom, per 66.7m2Chase after nitrogen,
Phosphorus, potassium 1~1.5kg of NPK, are applied 1~2 time, spray the foliar fertilizers such as 0.3% potassium dihydrogen phosphate extra-nutrition.It is in addition, warm
It is hot and humid in canopy after the greenhouse epiphragma of room, aphid, cabbage caterpillar, virosis, downy mildew, soft rot etc. easily occurs, need to prevent and treat in time.
Described above is only the preferred embodiment of the present invention, it should be understood that the present invention is not limited to described herein
Form, the exclusion to other embodiment is not to be taken as, and can be used for various other combinations, modification and environment, and can be at this
In the text contemplated scope, it is modified by the technology or knowledge of above-mentioned teaching or association area.And those skilled in the art are entered
Capable change and change does not depart from the spirit and scope of the present invention, then all should be in the protection domain of appended claims of the present invention
It is interior.
Claims (7)
1. a kind of Chinese cabbage parent greenhouse adds for mating system, it is characterised in that breeds step including following：
S1. by the first generation in the sowing of First Year late December and greenhouse, using 1:1 garden mould and vermiculite as culture medium,
18~22 DEG C of daytime in greenhouse, vernalization is carried out under the conditions of 3~8 DEG C of night；
S2. it is colonized in open country net canopy mid-March in Second Year, bolting is bloomed between Second Year 18 to April 22 April, then
Artificial pollination is carried out, terminates pollination after one month；
S3. in Second Year harvest mid-June parental seed；
S4. at the beginning of Second Year 7 months, after the abundant water swelling of seed, seed is put by seed soaking 4~4.5h in parental seed greenhouse
It is placed in the culture dish for being covered with double-layer filter paper, adds water to be sufficiently humidified so as to by filter paper, is placed in vernalization 11 in 20~25 DEG C of illumination boxs
~12h；
S5. after Seed sprouting, seed is placed under 0~5 DEG C of environment and carries out vernalization treatment 30~35 days；
S6. after vernalization terminates, seed was seeded into Second Year August to August in greenhouse in 5 within 1st, using 1:1 garden mould and
Vermiculite is as culture medium；
S7. 5~6 true leaves were colonized in Second Year August to August in greenhouse in 20 within 15th, in Second Year September 5 to September 10 days
Between bolting, start artificial cabage-type rape；
S8. film is capped in Second Year early November to improve warm indoor temperature, seed is fully ripe by the end of November in Second Year, receives
Obtain.
2. a kind of Chinese cabbage parent greenhouse according to claim 1 adds for mating system, it is characterised in that the step
In rapid S2 and S7, base fertilizer is used sufficient before field planting, per 66.7m2Apply high-quality farm manure 300kg, NPK 5kg, boron fertilizer 0.1
~0.2kg.
3. a kind of Chinese cabbage parent greenhouse according to claim 2 adds for mating system, it is characterised in that is being colonized
An instant nitrogenousfertilizer is chased after within 7~10 days afterwards carries seedling.
4. a kind of Chinese cabbage parent greenhouse according to claim 1 adds for mating system, it is characterised in that in bolting
Afterwards with before full blossom, per 66.7m2Nitrogen, phosphorus, potassium 1~1.5kg of NPK are chased after, is applied 1~2 time, sprays foliar fertilizer extra-nutrition.
5. a kind of Chinese cabbage parent greenhouse according to claim 4 adds for mating system, it is characterised in that the leaf
Leaven is 0.3% potassium dihydrogen phosphate.
6. a kind of Chinese cabbage parent greenhouse according to claim 1 adds for mating system, it is characterised in that adopts respectively
It is used to sow the first generation and parental seed with the hole tray in 32 or 50 holes.
7. a kind of Chinese cabbage parent greenhouse according to claim 1 adds for mating system, it is characterised in that is breeding
During periodically carry out disease control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711017653.0A CN107801628B (en) | 2017-10-27 | 2017-10-27 | A kind of Chinese cabbage parent greenhouse adds for mating system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711017653.0A CN107801628B (en) | 2017-10-27 | 2017-10-27 | A kind of Chinese cabbage parent greenhouse adds for mating system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107801628A true CN107801628A (en) | 2018-03-16 |
| CN107801628B CN107801628B (en) | 2019-07-26 |
Family
ID=61582218
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711017653.0A Expired - Fee Related CN107801628B (en) | 2017-10-27 | 2017-10-27 | A kind of Chinese cabbage parent greenhouse adds for mating system |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362505A (en) * | 2018-12-18 | 2019-02-22 | 武汉市农业科学院 | A kind of Chinese cabbage quickly adds the method in generation |
| CN109691370A (en) * | 2018-12-18 | 2019-04-30 | 武汉市农业科学院 | A kind of radish quickly adds the method in generation |
| CN111990103A (en) * | 2020-08-05 | 2020-11-27 | 江苏省农业科学院 | Method for adjusting flowering synchronization of hybrid seed production of non-heading Chinese cabbages |
| CN113597994A (en) * | 2021-08-19 | 2021-11-05 | 南京新创蔬菜分子育种研究院有限公司 | Vernalization treatment method of white stem vegetables |
-
2017
- 2017-10-27 CN CN201711017653.0A patent/CN107801628B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109362505A (en) * | 2018-12-18 | 2019-02-22 | 武汉市农业科学院 | A kind of Chinese cabbage quickly adds the method in generation |
| CN109691370A (en) * | 2018-12-18 | 2019-04-30 | 武汉市农业科学院 | A kind of radish quickly adds the method in generation |
| CN111990103A (en) * | 2020-08-05 | 2020-11-27 | 江苏省农业科学院 | Method for adjusting flowering synchronization of hybrid seed production of non-heading Chinese cabbages |
| CN113597994A (en) * | 2021-08-19 | 2021-11-05 | 南京新创蔬菜分子育种研究院有限公司 | Vernalization treatment method of white stem vegetables |
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| CN107801628B (en) | 2019-07-26 |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Song Juhong Inventor after: Xun Jiayu Inventor after: Wang Haishan Inventor after: Feng Zhiming Inventor after: Liu Yuqin Inventor before: Song Juhong Inventor before: Xun Jiayu |
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Effective date of registration: 20180904 Address after: 050021 No. 420, Ching yuan street, Yuhua District, Shijiazhuang, Hebei. Applicant after: SHIJIAZHUANG ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES Address before: 050021 No. 420, Ching yuan street, Yuhua District, Shijiazhuang, Hebei. Applicant before: Hu Yuezhan |
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Address after: 050041 479 Shengli North Street, Shijiazhuang, Hebei Applicant after: SHIJIAZHUANG ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES Address before: 050021 No. 420, Ching yuan street, Yuhua District, Shijiazhuang, Hebei. Applicant before: Shijiazhuang Academy of Agriculture and Forestry Sciences |
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Granted publication date: 20190726 Termination date: 20211027 |