CN109691370A - A kind of radish quickly adds the method in generation - Google Patents
A kind of radish quickly adds the method in generation Download PDFInfo
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- CN109691370A CN109691370A CN201811553502.1A CN201811553502A CN109691370A CN 109691370 A CN109691370 A CN 109691370A CN 201811553502 A CN201811553502 A CN 201811553502A CN 109691370 A CN109691370 A CN 109691370A
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- 235000006140 Raphanus sativus var sativus Nutrition 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 19
- 241000220259 Raphanus Species 0.000 title abstract description 24
- 241000196324 Embryophyta Species 0.000 claims abstract description 28
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 238000005286 illumination Methods 0.000 claims abstract description 13
- 241001290610 Abildgaardia Species 0.000 claims abstract description 10
- 230000010152 pollination Effects 0.000 claims abstract description 8
- 230000005059 dormancy Effects 0.000 claims abstract description 6
- 240000002853 Nelumbo nucifera Species 0.000 claims abstract description 4
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims abstract description 4
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 4
- 230000035800 maturation Effects 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 230000034303 cell budding Effects 0.000 claims description 3
- 230000003595 spectral effect Effects 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 2
- 244000088415 Raphanus sativus Species 0.000 claims 2
- 230000005562 seed maturation Effects 0.000 claims 1
- 238000001228 spectrum Methods 0.000 abstract description 5
- 230000002093 peripheral effect Effects 0.000 abstract 1
- 239000000463 material Substances 0.000 description 11
- 239000010977 jade Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 5
- 230000012010 growth Effects 0.000 description 4
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 150000002505 iron Chemical class 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000012266 salt solution Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 239000004254 Ammonium phosphate Substances 0.000 description 1
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- 229910000148 ammonium phosphate Inorganic materials 0.000 description 1
- 235000019289 ammonium phosphates Nutrition 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000407 epitaxy Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 230000010198 maturation time Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000014284 seed dormancy process Effects 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000009105 vegetative growth Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of methods that radish quickly adds generation, the following steps are included: seed sprout after 2-4 °C vernalization treatment 10-30 days, then it is transplanted to greenhouse, the whole day illumination cultivation under the LED lamp tube of full spectrum, plant controls plant type by way of removing peripheral old leaf and lotus throne leaf after buddingging, main a kind of sedge the flowers are in blossom put after pollinate to the mature bud of inflorescence outermost, the mature seeds removing to 25-30 days after pollination plants skins with breaking dormancy, after planting enters next generation.This method can greatly shorten the breeding time limit of radish, improve breeding efficiency, provide new method for radish high-efficient breeding.
Description
Technical field
The invention belongs to crop breeding fields, and in particular to a kind of to realize that radish quickly adds the new method in generation in greenhouse.
Background technique
Radish is biennial brassicaceous vegetable, can only breed a generation within 1 year under field conditions (factors).In breeding process
It cultivates a self-mating system and generally requires the 6-8 year, and cultivate a cenospecies and then need the longer time.In order to improve radish
Breeding efficiency explored quickly plus the method in generation there is an urgent need to shorten its growth cycle.Forefathers once attempted strange land and add generation, winter
Season greenhouse is collected seed and is bred radish material using means such as tissue cultures, but these methods can only achieve 1 year 1.5-2
The breeding in generation.Trace it to its cause be radish growth period it is longer, vegetative growth phase needs to experience the influence of vernalization, and the later period is also
Needing to incude higher temperature and long-day could normally bloom, or even mature seed also has stronger dormancy, so
The overlaying influence of these factors reproductive efficiency of radish.
Summary of the invention
In order to overcome the shortcomings and deficiencies of the prior art, the present invention provide it is a kind of using greenhouse carry out radish quickly add generation
Method, this method can greatly accelerate the breeding process of radish new varieties.Ring of the method using the seed sprouted at 2-4 DEG C
Vernalization treatment is carried out in border, is then transplanted under the LED lamp tube of the full spectrum in greenhouse and carries out continuous illumination in 24 hours, and temperature control exists
24-28 DEG C, accelerate the maturation time of radish seed in incubation by control plant type and Pollination Modes, by mature kind after pollination
The kind skin of son is removed with breaking dormancy, and next generation is after planting entered.
To achieve the goals above, the present invention is achieved through the following technical solutions:
A kind of radish quickly adds the method in generation, comprising the following steps:
1) seed sprouting and vernalization treatment
Selecting full seed adds water to sprout, and under the conditions of the seed sprouted after processing is put into 2-4 DEG C, carries out vernalization
Processing 10-30 days；
2) the plant high temperature long-day cultivates
It will complete the seed of vernalization treatment and carry out the culture of high temperature long-day in greenhouse, the LED lamp tube of Quan Guangpu lower 24 hours
Continuous illumination culture, spectral band is between 340-800nm, and temperature controls during 24-28 DEG C, culture, when at interval of two days
Between supplement a Hoagland ' s nutrient solution；
3) buds dwarfing
After plant buddings, the old leaf of periphery is removed, removes whole lotus throne leaves after bolting, inhibits side a kind of sedge to grow, most
Make plant only have main a kind of sedge to bloom eventually, accelerates plant maturation and bloom；
4) pollination is handled
When the main a kind of sedge of plant the flowers are in blossom put 3-4 after, to the mature bud of inflorescence outermost carry out artificial stripping flower bud selfing or
Remove stamen hybridization, so that it is normally set seeds, remove on inflorescence after the mature bud of inflorescence outermost can generate the silique of elongation
Immature bud；
5) seed harvest and breaking dormancy
When successfully silique of pollinating starts elongation, blade all on plant is destroyed, only retains silique, to accelerate
The maturation of seed is simultaneously stopped the supply of Hoagland ' s nutrient solution, and interval keeps the skin wet, and takes mature (after pollination 25-30 days)
Seed remove kind of a skin, and be seeded on culture dish, start the breeding of next generation.
Preferably, the vernalization treatment time is 20-30 days in step 1).
Compared with prior art, the present invention has the following advantages and beneficial effects:
The present invention comprehensively utilizes multiple technologies means and overcomes vernalization, illumination, temperature, strain according to the habit of radish
Influence of the factors such as type, seed dormancy to plant development finally realizes the quick adding generation of radish, greatly improves and educate
Kind efficiency.In the material for examination, material ' white jade spring ' the completion breeding time of strong winter habit only needs 105 days, the material of weak winter habit
' Duanye 13 ' then needs the less time, reaches 76 days.Therefore, radish will be realized extremely using method provided by the invention in greenhouse
The propagation generation in few 1 year 3-4 generation.
Specific embodiment
Below with reference to embodiment, the invention will be further described.
Radish material used in the present embodiment has two parts: portion is self-mating system ' white jade spring ' (big east gate in Wuhan of strong winter habit
Market purchase), another is the kind ' Duanye 13 ' (the big Dongmen Market purchase in Wuhan) of weak winter habit.
1. seed treatment
1.1 germinations: each 100, seed for selecting radish material ' white jade spring ' and ' Duanye 13 ' full seed use yarn
Cloth, which encases, is put into 50 DEG C of water the 15min that soaks seed, then seed is put into 6-7h in 25 DEG C of water, and seed is all exposed the bud of white, but son
Leaf is not yet unfolded.
The test of 1.2 vernalization treatment times: under the conditions of the seed sprouted is put into 2-4 DEG C, vernalization treatment is carried out
10d, 20d and 30d do not need illumination during processing.As a result, it has been found that the vernalization treatment time is longer, material bolting, needs of blooming
Time is shorter.In conjunction with the time of vernalization treatment, we show that the 20 days time of vernalization treatment can farthest shorten plant
Breeding time (table 1).
The breeding time of radish under the different vernalization time-triggered protocols of table 1.
2. plant is planted
2.1 sowings: after the completion of vernalization treatment, the seed of ' white jade spring ' and ' Duanye 13 ' reveals because of the reason of low-temperature epitaxy
Two panels cotyledon out, but be not fully extended.The seed of two materials is seeded into Nutrition Soil (purchased from the clear biological section in Wuhan day respectively
Skill company) in, each nutritive cube puts a seed, and watering makes its normal growth.Nutritive cube is placed on tissue culture frame, is made at its
Under the irradiation of fluorescent tube.
2.2 exposure experiments to light:
In order to determine the optimum illumination condition that can promote flowering of plant, we carry out test for light quality and illumination length,
The seed of ' white jade spring ' and ' Duanye 13 ' after vernalization 20 days is put into 4 incandescent lamp tubes (power 36W) respectively and 4 LED are complete
Spectrum fluorescent tube (from Shandong Gui Xiang Photoelectric Co., Ltd. customize, and adjust light quality be Quan Guangpu, spectral band 340-800nm it
Between, power 36W) composition tissue culture frame on.Then under two kinds of illumination conditions, light application time is set as 3 gradients, respectively
12 hours, 16 hours and 24 hours, the environment temperature in greenhouse was controlled at 25 DEG C.As a result, it has been found that (table 2) ' white jade spring ' and ' short leaf
Characteristic is experienced in 13 ' illumination having the same, meanwhile, under the conditions of identical light quality, light application time is longer, and the breeding time of plant gets over
It is short.Therefore, we determined that plant must can utmostly shorten radish under the conditions of continuous illumination in 24 hours of LED full spectrum fluorescent tube
Breeding time.
The breeding time of radish under 2. different illumination conditions of table (from the time spent is seeded into out)
The culture of 2.3 plant: it after germination is completed, puts it on the culturing rack of the full spectrum fluorescent tube composition of LED, carries out
Continuous illumination in 24 hours, until seed harvests.During culture, a Hoagland ' s nutrient solution was supplemented at interval of two day time
(formula are as follows: calcium nitrate 945mg/L, potassium nitrate 607mg/L, ammonium phosphate 115mg/L, magnesium sulfate 493mg/L, iron salt solutions
2.5ml/L, microelement 5ml/L, pH=6.0, wherein iron salt solutions: ferrous sulfate heptahydrate 2.78g, disodium ethylene diamine tetraacetate
3.73g, distilled water 500ml adjust pH=5.5；Liquid microelement: potassium iodide 0.83mg/l, boric acid 6.2mg/L, manganese sulfate
22.3mg/L, zinc sulfate 8.6mg/L, sodium molybdate 0.25mg/L, copper sulphate 0.025mg/L, cobalt chloride 0.025mg/L), greenhouse
Environment temperature is controlled at 25 DEG C.
3. buds dwarfing
After plant buddings, the old leaf of periphery is removed, to prevent its excessive growth, while showing that plant has been introduced into life
Generative growth phase.Remove whole lotus throne leaves of plant in the bolting stage, inhibits side a kind of sedge to grow, plant is finally made to only have main a kind of sedge to open
Flower.
4. pollination is handled
When the main a kind of sedge of plant the flowers are in blossom put 3-4 after, artificial stripping flower bud selfing is carried out to the mature bud of inflorescence outermost, makes it
Normally it can set seeds and mark.Retain immature bud on inflorescence simultaneously, to prevent the mature bud of periphery from cannot generate
Seed.After 5-7 days, remove all immature flowers on inflorescence after the mature bud of inflorescence outermost can generate the silique of elongation
Flower bud.
5. seed harvest and breaking dormancy
When successfully silique of pollinating starts elongation, blade all on plant is destroyed, only retains silique, to accelerate
The maturation of seed is simultaneously stopped the supply of Hoagland ' s nutrient solution, supplements a small amount of moisture every 5 days, and plant is made to be in opposite
In the environment of arid.After seed is mature (pollination 25 days), silique is removed, the kind skin of seed is removed, and is seeded on culture dish.
After 2-3 days, seed is sprouted, and cotyledon starts to grow, is placed into refrigerator and carries out vernalization treatment, starts the numerous of next generation
It grows.
In the material for examination, material ' white jade spring ' the completion breeding time of strong winter habit only needs 105 days, the material of weak winter habit
' Duanye 13 ' then needs the less time, reaches 76 days.Therefore, radish will be realized extremely using method provided by the invention in greenhouse
The propagation generation in few 1 year 3-4 generation.
Claims (2)
1. a kind of radish quickly adds the method in generation, which comprises the following steps:
1) seed sprouting and vernalization treatment
Selecting full seed adds water to sprout, and under the conditions of the seed sprouted after processing is put into 2-4 DEG C, carries out vernalization treatment
10-30 days；
2) the plant high temperature long-day cultivates
The seed for completing vernalization treatment is subjected to the culture of high temperature long-day in greenhouse, the LED lamp tube of Quan Guangpu lower 24 hours continuous
Illumination cultivation, between 340-800nm, temperature controls during 24-28 DEG C, culture spectral band, mends at interval of two day time
Fill a Hoagland ' s nutrient solution；
3) buds dwarfing
After plant buddings, the old leaf of periphery is removed, removes whole lotus throne leaves after bolting, inhibits side a kind of sedge to grow, finally makes
Plant only has main a kind of sedge to bloom, and accelerates plant maturation and blooms；
4) pollination is handled
When the main a kind of sedge of plant the flowers are in blossom put 3-4 after, artificial stripping flower bud selfing is carried out to the mature bud of inflorescence outermost or is removed
Stamen hybridization, makes it normally set seeds, remove on inflorescence after the mature bud of inflorescence outermost can generate the silique of elongation not at
Ripe bud；
5) seed harvest and breaking dormancy
When successfully silique of pollinating starts elongation, blade all on plant is destroyed, retains silique, only to accelerate seed
Maturation, be simultaneously stopped the supply of Hoagland ' s nutrient solution, interval keeps the skin wet, and silique is removed after seed is mature, by seed
Kind skin remove, and be seeded on culture dish, start the breeding of next generation.
2. radish according to claim 1 quickly adds the method in generation, which is characterized in that the vernalization treatment time is in step 1)
20-30 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811553502.1A CN109691370A (en) | 2018-12-18 | 2018-12-18 | A kind of radish quickly adds the method in generation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811553502.1A CN109691370A (en) | 2018-12-18 | 2018-12-18 | A kind of radish quickly adds the method in generation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109691370A true CN109691370A (en) | 2019-04-30 |
Family
ID=66231786
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811553502.1A Pending CN109691370A (en) | 2018-12-18 | 2018-12-18 | A kind of radish quickly adds the method in generation |
Country Status (1)
| Country | Link |
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| CN (1) | CN109691370A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583407A (en) * | 2019-10-18 | 2019-12-20 | 青岛市农业科学研究院 | Radish seed-replacing and reserving method |
| CN112450021A (en) * | 2020-12-07 | 2021-03-09 | 江苏沿海地区农业科学研究所 | Method for rapidly detecting purity of radish sterile line |
| CN114402924A (en) * | 2022-01-20 | 2022-04-29 | 中国农业科学院都市农业研究所 | Method for shortening whole growth period of lettuce by using LED illumination |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1484945A (en) * | 2002-09-27 | 2004-03-31 | 梅时勇 | Rapid breeding selection method of novel radish variety |
| CN107801628A (en) * | 2017-10-27 | 2018-03-16 | 胡月占 | A kind of Chinese cabbage parent greenhouse adds for mating system |
-
2018
- 2018-12-18 CN CN201811553502.1A patent/CN109691370A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1484945A (en) * | 2002-09-27 | 2004-03-31 | 梅时勇 | Rapid breeding selection method of novel radish variety |
| CN1214708C (en) * | 2002-09-27 | 2005-08-17 | 梅时勇 | Rapid breeding selection method of novel radish variety |
| CN107801628A (en) * | 2017-10-27 | 2018-03-16 | 胡月占 | A kind of Chinese cabbage parent greenhouse adds for mating system |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583407A (en) * | 2019-10-18 | 2019-12-20 | 青岛市农业科学研究院 | Radish seed-replacing and reserving method |
| CN112450021A (en) * | 2020-12-07 | 2021-03-09 | 江苏沿海地区农业科学研究所 | Method for rapidly detecting purity of radish sterile line |
| CN114402924A (en) * | 2022-01-20 | 2022-04-29 | 中国农业科学院都市农业研究所 | Method for shortening whole growth period of lettuce by using LED illumination |
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