CN107736283B - Energy-saving emission-reduction seedling raising method for blue robe sea urchins - Google Patents
Energy-saving emission-reduction seedling raising method for blue robe sea urchins Download PDFInfo
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- CN107736283B CN107736283B CN201710865106.1A CN201710865106A CN107736283B CN 107736283 B CN107736283 B CN 107736283B CN 201710865106 A CN201710865106 A CN 201710865106A CN 107736283 B CN107736283 B CN 107736283B
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- 241000257465 Echinoidea Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 36
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 50
- 239000013535 sea water Substances 0.000 claims abstract description 24
- 241000894006 Bacteria Species 0.000 claims abstract description 17
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 17
- 230000008569 process Effects 0.000 claims abstract description 17
- 241000091751 Chaetoceros muellerii Species 0.000 claims abstract description 12
- 238000005286 illumination Methods 0.000 claims abstract description 9
- 235000013601 eggs Nutrition 0.000 claims description 26
- 210000000232 gallbladder Anatomy 0.000 claims description 24
- 230000003203 everyday effect Effects 0.000 claims description 16
- 241000334163 Amphiprion percula Species 0.000 claims description 10
- 150000003839 salts Chemical class 0.000 claims description 9
- 230000029052 metamorphosis Effects 0.000 claims description 8
- 241000206761 Bacillariophyta Species 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 230000002354 daily effect Effects 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 230000001720 vestibular Effects 0.000 claims description 6
- 238000010521 absorption reaction Methods 0.000 claims description 4
- 230000007547 defect Effects 0.000 claims description 4
- 241000196252 Ulva Species 0.000 claims description 3
- 238000003314 affinity selection Methods 0.000 claims description 3
- 210000000941 bile Anatomy 0.000 claims description 3
- 230000001939 inductive effect Effects 0.000 claims description 3
- 210000002784 stomach Anatomy 0.000 claims description 3
- 238000009395 breeding Methods 0.000 abstract description 17
- 230000001488 breeding effect Effects 0.000 abstract description 17
- 238000005265 energy consumption Methods 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 4
- 239000002351 wastewater Substances 0.000 abstract description 4
- 230000009471 action Effects 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 23
- 241000251468 Actinopterygii Species 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 241000227752 Chaetoceros Species 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000003337 fertilizer Substances 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000009182 swimming Effects 0.000 description 2
- 210000000707 wrist Anatomy 0.000 description 2
- 241000700104 Brachionus plicatilis Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000258955 Echinodermata Species 0.000 description 1
- 235000001715 Lentinula edodes Nutrition 0.000 description 1
- 240000000599 Lentinula edodes Species 0.000 description 1
- 241000416015 Orthomorpha Species 0.000 description 1
- 241000258128 Strongylocentrotus purpuratus Species 0.000 description 1
- 241000677635 Tuxedo Species 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003238 esophagus Anatomy 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000037406 food intake Effects 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000035929 gnawing Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/30—Culture of aquatic animals of sponges, sea urchins or sea cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/06—Arrangements for heating or lighting in, or attached to, receptacles for live fish
- A01K63/065—Heating or cooling devices
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for breeding blue robe sea urchins, which can breed a large amount of blue robe sea urchin seedlings under the conditions of extremely low energy consumption and culture wastewater discharge so as to achieve the purposes of reducing the pressure of natural resource collection and catching, solving the problems of high transportation cost, low survival rate and the like. Firstly, the breeding environment is placed in a greenhouse, the water temperature of the breeding water body is kept in a relatively high range under the action of the greenhouse, artificial temperature rise seawater is not used for breeding seedlings, and the pollution of a boiler to the environment is avoided on the premise of saving the cost; meanwhile, in the cultivation process, the larvae are cultivated by adopting illumination within the range of 1500-2500 lx, and Chaetoceros muelleri and seawater photosynthetic bacteria are put in, so that the purpose of purifying water quality can be achieved, and the cost problem caused by a large amount of frequent water change is avoided.
Description
Technical Field
The invention relates to the field of sea urchin breeding, in particular to an energy-saving and emission-reduction seedling raising method for blue robe sea urchins.
Background
Blue full dress sea urchinMespilia globulus) Belonging to Echinodermata, Hemicentridae, Orthomorpha, Arcostaphanidae, the English name Blue Tuxedo Urchin. Distributed in the Indian ocean, the Pacific ocean, and the south China sea up to the south Japan sea area. The shell of the blue ceremonial sea urchin has five wide blue or black textures, the spines are red, brown or black, the color matching is very beautiful, the ornamental value is high, and the sea urchin can help gnawing the algae in the aquarium, so that the sea urchin is widely cultured in the seawater aquarium.
The market demand of blue full-dress sea urchins is very large, the blue full-dress sea urchins sold in the domestic aquarium market at present are naturally fished in hot zone sea areas such as Indian ocean and the like and transported to the destination in an air mode, and the cost of fishing and transporting is high, so that the selling price of one blue full-dress sea urchin can reach dozens of yuan or even more than one hundred yuan. And long-distance transportation reduces the survival rate of the fish, and brings great economic loss to consumers. The most effective method for solving the problems is to carry out artificial breeding of the blue full-dress sea urchins in China. However, there is no report on artificial breeding of blue robe sea urchins, and although the existing cold water-based sea urchin (such as shiitake sea urchins) breeding technology and tropical sea urchins (such as purple sea urchins) breeding technology can be used as references, the conditions of water temperature, bait and the like required by different types of sea urchins are greatly different, and the fact that the blue robe sea urchin breeding is still not successful by using other types of existing technologies is found in practice. In addition, blue robe sea urchins are tropical, high-temperature seawater (25-30 ℃) is needed in the seedling cultivation process, if artificial breeding is carried out in the north and breeding facilities and water changing methods (1/3-1/2 for each time, 1-2 times a day) adopted by other sea urchins are adopted, a large amount of energy needs to be consumed to heat the seawater, so that the energy consumption and the cost in the seedling cultivation process are greatly increased, and the environment is polluted by boiler burning or waste water discharge and the like.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a seedling culture method which can culture a large number of blue formal sea urchin seedlings under the conditions of extremely low energy consumption and culture wastewater discharge so as to reduce the pressure of natural resource collection and capture and solve the problems of high transportation cost, low survival rate and the like.
The technical solution of the invention is as follows: an energy-saving emission-reduction seedling raising method for blue robe sea urchins is characterized by comprising the following steps: the method comprises the steps of parent gall selection, fertilized egg obtaining, planktonic larva cultivation, larva metamorphosis and attachment, larva cultivation, larva peeling and intermediate cultivation, wherein the steps are all carried out in a greenhouse, the top of the greenhouse is provided with a movable sunshade net, the side surface of the greenhouse is provided with an openable or closable vent, in the operation process of the method, the temperature range in the greenhouse is 25-40 ℃, the water temperature range in a larva cultivation pool in the greenhouse is 25-30 ℃,
the steps of gallbladder affinity selection are as follows: mature sea urchin with shell diameter of more than 3cm, good vitality and no surface defect is selected as parent sea urchin,
the fertilized egg obtaining step comprises: inducing the parent bile to lay eggs and discharge sperms, mixing the sperms and the sperms to obtain fertilized eggs, putting the fertilized eggs into seawater at the temperature of 25-28 ℃ for incubation, wherein the incubation density is 20-30/mL, the eggs are washed once when the fertilized eggs are fertilized for 1 hour, the eggs are washed once again when the fertilized eggs are fertilized for 2 hours, and the selection is preferably carried out,
the cultivation steps of the planktonic larvae are as follows: putting the selected larvae into a culture pond for culture, wherein the culture density is 0.5-0.8/mL, feeding bait after the prism period, feeding Chaetoceros muelleri 5000-10000 cells/mL and seawater photosynthetic bacteria 2000 cells/mL every day before the four-wrist larvae are finished, feeding Chaetoceros muelleri 10000-20000 cells/mL, seawater photosynthetic bacteria 2000 cells/mL and Rotenoides plicatilis 20 cells/mL every day during the six-wrist larvae period, feeding Chaetoceros muelleri 20000-30000 cells/mL, seawater photosynthetic bacteria 2000 cells/mL and Rotenoides plicatilis 40 cells/mL every day during the eight-wrist larvae period, adding water after bottom suction by a siphon tube every 2 days, wherein the water absorption amount is one tenth of the water amount of the whole pond,
the metamorphosis and attachment steps of the larvae are as follows: when the larvae grow to the later stage of the eight-wrist larvae, a vestibular complex appears on the stomach side, when 5-10% of individual tuba protrudes out of the vestibular cavity wall, all the larvae are collected and then moved into a culture pond with corrugated plates, and the culture density is 0.2-0.4/cm2The corrugated plate is inoculated with benthic diatoms in advance, the illumination is 1500-2500 lx in the cultivation process, the water temperature is 25-30 ℃, the water is changed for 1 time in 5-7 days, and 5-8 clown fishes with the body length of 2-3 cm are thrown in the cultivation process3When larvae are thrown in, the corrugated plate is horizontally placed, the corrugated plate is turned 180 degrees after 1-2 days and placed, the corrugated plate is vertically placed after 5 days, benthic diatom begins to be fed into the culture pond after the corrugated plate is vertically placed, the benthic diatom is fed once every day, 600 cells/mL are fed every time for 10 days continuously,
in the step of cultivating the young gall bladder, nutrient salt is added into the cultivating pool, the amount of the added nutrient salt is N: P: Si: Fe = 2: 0.1: 0.01ppm daily, the illumination intensity is controlled below 3000Lux, the natural photoperiod,
the steps of the detachment and the intermediate cultivation of the young gall bladder are as follows: after the young gall bladder grows on the corrugated plate for 1 month, peeling the young gall bladder into a water tank which is provided with a black corrugated plate and a temperature control device by using a soft brush for intermediate cultivation, feeding ulva into the water tank once every 2-3 days, adding seawater photosynthetic bacteria according to the amount of 2000 cells/mL every day, and when the diameter of the shell of the young gall bladder is less than 0.5cm, cultivating the young gall bladder at the density of 5000 cells/m2When the diameter of the young gallbladder shells is more than 0.5cm, the cultivation density is 2000 pieces/m2During the intermediate cultivation period, the water temperature is controlled at 26-30 ℃, the salinity is 29-30 per mill, and the pH value is 7.5-8.0.
Compared with the prior art, the invention has the following advantages:
compared with the traditional sea urchin seedling culture method, the method for culturing the blue robe sea urchin seedlings disclosed by the invention can be used for culturing a large number of blue robe sea urchin seedlings under the conditions of extremely low energy consumption and culture wastewater discharge so as to achieve the purposes of reducing the pressure of natural resource collection and capture, solving the problems of high transportation cost, low survival rate and the like. Firstly, the breeding environment is placed in a greenhouse, the water temperature of the breeding water body is kept in a relatively high range under the action of the greenhouse, artificial temperature rise seawater is not used for breeding seedlings, and the pollution of a boiler to the environment is avoided on the premise of saving the cost; meanwhile, in the cultivation process, the larvae are cultivated by adopting illumination within the range of 1500-2500 lx, and Chaetoceros muelleri and seawater photosynthetic bacteria are put in, so that the purpose of purifying water quality can be achieved, and the cost problem caused by a large amount of frequent water change is avoided; and the clown fish is put into the culture pond in the metamorphosis and attachment process of the larva, the applied nutritive salt is uniformly distributed in the water body by utilizing the swimming of the fish, meanwhile, the clown fish can eat protozoa eating the benthic diatom plate, and the produced excrement can also be used as the fertilizer of the benthic diatom; in conclusion, the method has multiple advantages, is particularly suitable for popularization and application in the field, and has a very wide market prospect.
Detailed Description
The following will explain specific embodiments of the present invention.
An energy-saving emission-reduction seedling method for sea urchins in blue robe clothes comprises the steps of parent urchin selection, germ cell obtaining, cultivation of floating larvae, metamorphosis and attachment of larvae, cultivation of young urchins, peeling of young urchins and intermediate cultivation, wherein the steps are all carried out in a greenhouse, the top of the greenhouse is provided with a movable sunshade net, meanwhile, the side surface of the greenhouse is provided with an openable or closable vent, in the operation process of the method, the temperature range in the greenhouse is 25-40 ℃, the water temperature range in a larva cultivation pool in the greenhouse is 25-30 ℃,
the steps of gallbladder affinity selection are as follows: mature sea urchin with shell diameter of more than 3cm, good vitality and no surface defect is selected as parent sea urchin,
the fertilized egg obtaining step comprises: inducing the parent bile to lay eggs and discharge sperms, mixing the sperms and the sperms to obtain fertilized eggs, putting the fertilized eggs into seawater at the temperature of 25-28 ℃ for incubation, wherein the incubation density is 20-30/mL, the eggs are washed once when the fertilized eggs are fertilized for 1 hour, the eggs are washed once again when the fertilized eggs are fertilized for 2 hours, and the selection is preferably carried out,
the cultivation steps of the planktonic larvae are as follows: putting the selected larvae into a culture pond for culture, wherein the culture density is 0.5-0.8/mL, feeding bait after the prism period, feeding Chaetoceros muelleri 5000-10000 cells/mL and seawater photosynthetic bacteria 2000 cells/mL every day before the four-wrist larvae are finished, feeding Chaetoceros muelleri 10000-20000 cells/mL, seawater photosynthetic bacteria 2000 cells/mL and Rotenoides plicatilis 20 cells/mL every day during the six-wrist larvae period, feeding Chaetoceros muelleri 20000-30000 cells/mL, seawater photosynthetic bacteria 2000 cells/mL and Rotenoides plicatilis 40 cells/mL every day during the eight-wrist larvae period, adding water after bottom suction by a siphon tube every 2 days, wherein the water absorption amount is one tenth of the water amount of the whole pond,
the metamorphosis and attachment steps of the larvae are as follows: when the larvae grow to the later stage of the eight-wrist larvae, a vestibular complex appears on the stomach side, when 5-10% of individual tuba protrudes out of the vestibular cavity wall, all the larvae are collected and then moved into a culture pond with corrugated plates, and the culture density is 0.2-0.4/cm2The corrugated plate is inoculated with benthic diatoms in advance, the illumination is 1500-2500 lx in the cultivation process, the water temperature is 25-30 ℃, the water is changed for 1 time in 5-7 days, and the cultivation process is implemented5-8 clown fishes with the body length of 2-3 cm are put in the fish culture pond3When larvae are thrown in, the corrugated plate is horizontally placed, the corrugated plate is turned 180 degrees after 1-2 days and placed, the corrugated plate is vertically placed after 5 days, benthic diatom begins to be fed into the culture pond after the corrugated plate is vertically placed, the benthic diatom is fed once every day, 600 cells/mL are fed every time for 10 days continuously,
in the step of cultivating the young gall bladder, nutrient salt is added into the cultivating pool, the amount of the added nutrient salt is N: P: Si: Fe = 2: 0.1: 0.01ppm daily, the illumination intensity is controlled below 3000Lux, the natural photoperiod,
the steps of the detachment and the intermediate cultivation of the young gall bladder are as follows: after the young gall bladder grows on the corrugated plate for 1 month, peeling the young gall bladder into a water tank which is provided with a black corrugated plate and a temperature control device by using a soft brush for intermediate cultivation, feeding ulva into the water tank once every 2-3 days, adding seawater photosynthetic bacteria according to the amount of 2000 cells/mL every day, and when the diameter of the shell of the young gall bladder is less than 0.5cm, cultivating the young gall bladder at the density of 5000 cells/m2When the diameter of the young gallbladder shells is more than 0.5cm, the cultivation density is 2000 pieces/m2During the intermediate cultivation period, the water temperature is controlled at 26-30 ℃, the salinity is 29-30 per mill, and the pH value is 7.5-8.0.
If the traditional method is adopted for breeding the blue formal sea urchins, energy consumption and environment pollution can be increased due to the fact that a large amount of water needs to be changed, in addition, the long-wrist larvae, the back wrists and the back wrists of the blue formal sea urchins are wide, the water changing or pond pouring operation is easier to be damaged mechanically than other sea urchins, and once the damage is caused, the blue formal sea urchins die quickly, and the breeding failure is caused. Aiming at the problem, the method adopts light within the range of 1500-2500 lx to culture the larvae, and puts in Chaetoceros muelleri and seawater photosynthetic bacteria. On one hand, the chaetoceros and the photosynthetic bacteria can be used as baits, and on the other hand, under the illumination, the chaetoceros and the photosynthetic bacteria can carry out photosynthesis by using inorganic and organic nutritive salts generated by the degradation of excrement and residual baits, thereby achieving the purpose of purifying water quality. Practice proves that under the conditions, water can not be changed for a long time, or only a siphon is used for absorbing bottom every day, the water absorption amount only needs to reach about one tenth, and all water quality indexes in the seedling raising pond can be maintained at normal level.
In addition, in the cultivation process of planktonic larvae, when blue formal sea urchin larvae grow to six-wrist larvae, the esophagus of the larvae is increased to 20-30 um, so that Brachionus plicatilis is added into the bait in the step, the ingestion effect of the larvae is improved, and the nutritional requirements of the larvae are guaranteed;
in the whole process of the method, the temperature of the greenhouse is kept at 25-40 ℃ by utilizing the opening and closing of the sunshade net and the ventilation opening in the greenhouse, so that the artificial temperature rise of seawater for seedling culture is avoided, and the cost is saved; the pollution to the environment caused by burning the boiler is also avoided;
in the metamorphosis and attachment steps of the larvae, the corrugated plates need to be inoculated with benthic diatoms in advance, and the culture conditions are different from the conventional conditions mainly by the following points: the illumination is 1500-2500 lx, the water temperature is 25-30 ℃, water is not flowed, the water is changed for 1 time in 5-7 days, and 5-8 clown fishes with the body length of 2-3 cm are thrown in the culture process3. The purpose of throwing the clown fish is that the applied nutrient salt is uniformly distributed in the water body through the swimming of the fish, the clown fish can eat protozoa on the benthic diatom plate, the excrement produced by the clown fish can be used as fertilizer of the benthic diatom, and the benthic diatom can grow well and quickly by throwing the clown fish;
after the corrugated plate for collecting the seedlings is vertically placed, feeding other benthic diatoms which are not thrown onto the corrugated plate of the seedling pool and washed off into water is started, the benthic diatoms are fed once a day, 500-1000 cells/mL are fed every time, and the benthic diatoms are continuously fed for 10 days.
Claims (1)
1. An energy-saving emission-reduction seedling raising method for blue robe sea urchins is characterized by comprising the following steps: the method comprises the steps of parent gall selection, fertilized egg obtaining, planktonic larva cultivation, larva metamorphosis and attachment, larva cultivation, larva peeling and intermediate cultivation, wherein the steps are all carried out in a greenhouse, the top of the greenhouse is provided with a movable sunshade net, the side surface of the greenhouse is provided with an openable or closable vent, in the operation process of the method, the temperature range in the greenhouse is 25-40 ℃, the water temperature range in a larva cultivation pool in the greenhouse is 25-30 ℃,
the steps of gallbladder affinity selection are as follows: mature sea urchin with shell diameter of more than 3cm, good vitality and no surface defect is selected as parent sea urchin,
the fertilized egg obtaining step comprises: inducing the parent bile to lay eggs and discharge sperms, mixing the sperms and the sperms to obtain fertilized eggs, putting the fertilized eggs into seawater at the temperature of 25-28 ℃ for incubation, wherein the incubation density is 20-30/mL, the eggs are washed once when the fertilized eggs are fertilized for 1 hour, the eggs are washed once again when the fertilized eggs are fertilized for 2 hours, and the selection is preferably carried out,
the cultivation steps of the planktonic larvae are as follows: putting the selected larvae into a culture pond for culture, wherein the culture density is 0.5-0.8/mL, feeding bait after the prism period, feeding Chaetoceros muelleri 5000-10000 cells/mL and seawater photosynthetic bacteria 2000 cells/mL every day before the four-wrist larvae are finished, feeding Chaetoceros muelleri 10000-20000 cells/mL, seawater photosynthetic bacteria 2000 cells/mL and Rotenoides plicatilis 20 cells/mL every day during the six-wrist larvae period, feeding Chaetoceros muelleri 20000-30000 cells/mL, seawater photosynthetic bacteria 2000 cells/mL and Rotenoides plicatilis 40 cells/mL every day during the eight-wrist larvae period, adding water after bottom suction by a siphon tube every 2 days, wherein the water absorption amount is one tenth of the water amount of the whole pond,
the metamorphosis and attachment steps of the larvae are as follows: when the larvae grow to the later stage of the eight-wrist larvae, a vestibular complex appears on the stomach side, when 5-10% of individual tuba protrudes out of the vestibular cavity wall, all the larvae are collected and then moved into a culture pond with corrugated plates, and the culture density is 0.2-0.4/cm2The corrugated plate is inoculated with benthic diatoms in advance, the illumination is 1500-2500 lx in the cultivation process, the water temperature is 25-30 ℃, the water is changed for 1 time in 5-7 days, and 5-8 clown fishes with the body length of 2-3 cm are thrown in the cultivation process3When larvae are thrown in, the corrugated plate is horizontally placed, the corrugated plate is turned 180 degrees after 1-2 days and placed, the corrugated plate is vertically placed after 5 days, benthic diatom begins to be fed into the culture pond after the corrugated plate is vertically placed, the benthic diatom is fed once every day, 600 cells/mL are fed every time for 10 days continuously,
in the step of cultivating the young gall bladder, nutrient salt is added into the cultivating pool, and the amount of the added nutrient salt is as follows: n =2ppm daily, P =0.1ppm daily, Si =0.1ppm daily, Fe =0.01ppm daily, light intensity controlled to 3000Lux or less, natural light cycle,
the steps of the detachment and the intermediate cultivation of the young gall bladder are as follows: after the young gall bladder grows on the corrugated plate for 1 month, peeling the young gall bladder into a water tank which is provided with a black corrugated plate and a temperature control device by using a soft brush for intermediate cultivation, feeding ulva into the water tank once every 2-3 days, adding seawater photosynthetic bacteria according to the amount of 2000 cells/mL every day, and when the diameter of the shell of the young gall bladder is less than 0.5cm, cultivating the young gall bladder at the density of 5000 cells/m2When the diameter of the young gallbladder shells is more than 0.5cm, the cultivation density is 2000 pieces/m2During the intermediate cultivation period, the water temperature is controlled at 26-30 ℃, the salinity is 29-30 per mill, and the pH value is 7.5-8.0.
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| WO2008074084A1 (en) * | 2006-12-20 | 2008-06-26 | Macquarie University | Production of sea urchin roe |
| CN101940171A (en) * | 2010-07-26 | 2011-01-12 | 大连太平洋海珍品有限公司 | Breeding method of glyptocidaris crenularis offspring seed |
| CN105766707A (en) * | 2016-02-01 | 2016-07-20 | 大连海洋大学 | Cultivation method for high temperature resistance sea urchins |
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2017
- 2017-09-22 CN CN201710865106.1A patent/CN107736283B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008074084A1 (en) * | 2006-12-20 | 2008-06-26 | Macquarie University | Production of sea urchin roe |
| CN101940171A (en) * | 2010-07-26 | 2011-01-12 | 大连太平洋海珍品有限公司 | Breeding method of glyptocidaris crenularis offspring seed |
| CN105766707A (en) * | 2016-02-01 | 2016-07-20 | 大连海洋大学 | Cultivation method for high temperature resistance sea urchins |
Non-Patent Citations (1)
| Title |
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| 中间球海胆"大金";常亚青;《中国水产》;20151231(第12期);第58-59页 * |
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