CN105766707A - Cultivation method for high temperature resistance sea urchins - Google Patents
Cultivation method for high temperature resistance sea urchins Download PDFInfo
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- CN105766707A CN105766707A CN201610070062.9A CN201610070062A CN105766707A CN 105766707 A CN105766707 A CN 105766707A CN 201610070062 A CN201610070062 A CN 201610070062A CN 105766707 A CN105766707 A CN 105766707A
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- 241000257465 Echinoidea Species 0.000 title abstract description 9
- 238000012364 cultivation method Methods 0.000 title abstract 3
- 238000009395 breeding Methods 0.000 claims abstract description 25
- 241000258129 Strongylocentrotus intermedius Species 0.000 claims abstract description 24
- 241000258128 Strongylocentrotus purpuratus Species 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 23
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 238000011534 incubation Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 38
- 241000258149 Hemicentrotus Species 0.000 claims description 30
- 241000258125 Strongylocentrotus Species 0.000 claims description 30
- 210000000232 gallbladder Anatomy 0.000 claims description 21
- 235000013372 meat Nutrition 0.000 claims description 21
- 210000004681 ovum Anatomy 0.000 claims description 18
- 239000007788 liquid Substances 0.000 claims description 13
- 102000002322 Egg Proteins Human genes 0.000 claims description 11
- 108010000912 Egg Proteins Proteins 0.000 claims description 11
- 241000199919 Phaeophyceae Species 0.000 claims description 9
- 235000015170 shellfish Nutrition 0.000 claims description 9
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 6
- 210000000625 blastula Anatomy 0.000 claims description 6
- 210000004392 genitalia Anatomy 0.000 claims description 6
- 210000004602 germ cell Anatomy 0.000 claims description 6
- 210000004907 gland Anatomy 0.000 claims description 6
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 230000032696 parturition Effects 0.000 claims description 6
- 239000013535 sea water Substances 0.000 claims description 6
- 230000012447 hatching Effects 0.000 claims description 5
- 238000010792 warming Methods 0.000 claims description 5
- 210000000476 body water Anatomy 0.000 claims description 4
- 238000009313 farming Methods 0.000 claims description 4
- 238000007667 floating Methods 0.000 claims description 4
- 230000000630 rising effect Effects 0.000 claims description 4
- 241000237536 Mytilus edulis Species 0.000 claims description 3
- 241000237509 Patinopecten sp. Species 0.000 claims description 3
- 238000011161 development Methods 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 210000003608 fece Anatomy 0.000 claims description 3
- 210000002149 gonad Anatomy 0.000 claims description 3
- 238000011084 recovery Methods 0.000 claims description 3
- 235000020637 scallop Nutrition 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 230000003442 weekly effect Effects 0.000 claims description 3
- 210000000582 semen Anatomy 0.000 claims description 2
- 230000004083 survival effect Effects 0.000 abstract description 5
- 230000009027 insemination Effects 0.000 abstract description 2
- 210000002257 embryonic structure Anatomy 0.000 abstract 1
- 230000006698 induction Effects 0.000 abstract 1
- 230000005070 ripening Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 2
- 241000512259 Ascophyllum nodosum Species 0.000 description 1
- 241000258127 Mesocentrotus nudus Species 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Farming Of Fish And Shellfish (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Animal Behavior & Ethology (AREA)
Abstract
The invention discloses a cultivation method for high temperature resistance sea urchins. Female purple sea urchins and male strongylocentrotus intermedius are used as breeding urchins, and hybrid urchins are obtained through the steps of breeding urchin ripening promotion, spawning induction, ova collection, sperm collection, insemination, incubation, selection, breeding and the like. The most important character of the method is reflected in the ova and sperm collection way, temperature adjustment treatment needs to be carried out on collected ova and sperms, a wave making pump is adopted in the incubation process so as to obtain hybrid embryos and improve the incubation rate, hybrid sea urchins having the better high temperature resistance compared with male and female parents are obtained, and the cultivation method has the important significance in enlarging the sea urchin cultivation area and increasing the survival rate of the sea urchins in the high temperature environment.
Description
Technical field
The present invention relates to the breeding method of a kind of Hemicentrotus seu Strongylocentrotus, particularly the breeding method of a kind of high temperature resistant Hemicentrotus seu Strongylocentrotus.
Background technology
Hemicentrotus seu Strongylocentrotus due to delicious flavour, be of high nutritive value and deeply by the liking of the people of other countries, Hemicentrotus seu Strongylocentrotus naturally fished can not meet the demand in market already, therefore, interior cultivation interest and the cultured area to Hemicentrotus seu Strongylocentrotus of world wide just increases year by year.Northern China Hemicentrotus seu Strongylocentrotus economic species mainly has Strongylocentrotus intermedius, horsedung sea urchin, Strongylocentrotus nudus and sea Rrinaceus earopaeus etc., and the main aquaculture method of these kinds is for broadcasting propagation at the bottom of marine suspended longline farming and seabed.When carrying out the cultivation of Hemicentrotus seu Strongylocentrotus in the north, its marine suspended longline farming area is less, its main cause is that the high water temperature (often reaching more than 30 DEG C) in mariculture area summer is considerably beyond any north Hemicentrotus seu Strongylocentrotus the highest tolerant water temperature, the survival of Hemicentrotus seu Strongylocentrotus in serious threat, therefore, a kind of resistant to elevated temperatures Hemicentrotus seu Strongylocentrotus new varieties are cultivated very necessary for the cultured area expanding Hemicentrotus seu Strongylocentrotus.
Summary of the invention
The present invention is to solve the above-mentioned deficiency existing for prior art, it is proposed to one can obtain heat-resisting ability, to improve the breeding method of the high temperature resistant Hemicentrotus seu Strongylocentrotus of the survival rate of Hemicentrotus seu Strongylocentrotus raft culture.
The technical solution of the present invention is: the breeding method of a kind of high temperature resistant Hemicentrotus seu Strongylocentrotus, it is characterised in that: described method carries out according to following steps:
A. using female purple sea urchin and male Strongylocentrotus intermedius as kind of a gallbladder, the quality of purple sea urchin is be more than or equal to 60g, and the quality of Strongylocentrotus intermedius, be more than or equal to 50g, is planted gallbladder and required anosis, body surface not damaged,
B. respectively purple sea urchin is placed in male Strongylocentrotus intermedius support 3-10 days temporary with under locality identical water temperature, then breeding water body is progressively heated up, every day, increasing extent of temperature was 0.5 DEG C, the breeding water body water temperature of Strongylocentrotus intermedius stops heating up and being maintained at this temperature after rising to 15 ± 1 DEG C, the breeding water body water temperature of purple sea urchin stops heating up and being maintained at this temperature after rising to 22 ± 1 DEG C
C. purple sea urchin and Strongylocentrotus intermedius is raised using Brown algae and the shellfish meat boiled as bait, the time scale thrown something and fed for after three days Brown algaes of often throwing something and feeding, one day shellfish meat of throwing something and feeding, Brown algae is fresh Thallus Laminariae (Thallus Eckloniae) or Thallus Laminariae, shellfish meat is Mytilus edulis meat, clam son's meat, scallop meat or partially pushes up clam meat
D., breeding process removes Hemicentrotus seu Strongylocentrotus feces and dead individuals at any time, keeps water quality pure and fresh, within every 1-2 days, change water once, fall weekly pond once, and change water and fall the water temperature temperature difference before and after pond lower than 1 DEG C,
E. from support temporarily terminate after start at 1 month after, randomly selected purple sea urchin and Strongylocentrotus intermedius 3-5 every 7 days only, measure liveweight, and measure gonad weight in wet base after dissecting, calculate genital gland indices, when genital gland indices reaches more than 20%, it is believed that it is ripe to plant gallbladder,
Kind of a gallbladder is hastened parturition by the mode f. adopting injection Klorvess Liquid, and the concentration of Klorvess Liquid used is 0.5mol/L, and the injection dosage of every kind gallbladder is 1-2mL,
G. the anti-actinal surface of female purple sea urchin starting to lay eggs is individually placed in down the taper bottle mouth position filling sea water, recovery of ova, and the water temperature collected of ovum be 25 ± 1 DEG C,
H. in desiccation culture ware, lay 1-2 metafiltration paper, the anti-actinal surface of male Strongylocentrotus intermedius starting to produce essence be individually placed in culture dish down, collect sperm, and the temperature of culture dish is 18 ± 1 DEG C, and the sperm collected at the uniform velocity is warming up to 22 ± 1 DEG C in 30min,
I. being put in the ovum liquid after g step process by the sperm after h step process, and stir 1-3 minute, it is thus achieved that germ cell, the ratio between pure seminal fluid and ovum liquid is 1:1000,
J. germ cell being poured in the sea water of 20-40L, hatching density is 50/mL, hatches water temperature 25 ± 1 DEG C, and what configure 1-3W in incubation vessel makes wave pump, makes wave pump and is always on, close and make wave pump after blastula stage in the process of development of fertilized ova to blastula stage,
K. after germling floats, utilizing the bolting silk net of 300 orders to be pulled out by the germling of floating, and be invested in nursery pond and cultivate, cultivating water temperature is 26 ± 1 DEG C, and culture density is 0.2-0.5/mL,
L. the shell Diameter growth of seed is to 1cm, carries out pond culture or suspended longline farming, the high temperature resistant Hemicentrotus seu Strongylocentrotus of final acquisition.
The present invention compared with the existing technology, has the advantage that
Hemicentrotus seu Strongylocentrotus breeding method disclosed in this invention, using female purple sea urchin and male Strongylocentrotus intermedius as kind of a gallbladder, through kind of a gallbladder accelerating, hasten parturition, ova collection, sperm collection, insemination, hatching, selection-breeding, the steps such as larval rearing, obtain the Hemicentrotus seu Strongylocentrotus of a kind of hybridization, what the method was maximum is characterized in that the collection method of sperm and ovum and needs it is carried out homoiothermic process after collecting sperm and ovum, and hatching process adopts and makes wave pump, so can obtain hybridization embryo, and improve incubation rate, obtain relatively its male parent, female parent is respectively provided with the hybridization Hemicentrotus seu Strongylocentrotus of relatively high temperature tolerance, for expanding Hemicentrotus seu Strongylocentrotus cultured area, increase Hemicentrotus seu Strongylocentrotus survival rate in high temperature environments, have great importance.
Detailed description of the invention
The specific embodiment of the present invention is described below.
In December, 2014, fishes purple sea urchin from ALONG COASTAL FUJIAN, and selects female as planting gallbladder, locality water temperature 18 DEG C from which, and male Strongylocentrotus intermedius kind gallbladder is from north marine culture and stock enhancement key lab of the Ministry of Agriculture of Dalian Ocean University, temperature of cultivation 12 DEG C.Purple sea urchin more than liveweight 65g, Strongylocentrotus intermedius more than weight 55g, two kinds of Hemicentrotus seu Strongylocentrotuss are all anosis, body surface not damaged, and pipe foot is strong.
Above-mentioned kind of gallbladder collection is supported and accelerating temporarily to north marine culture and stock enhancement key lab of the Ministry of Agriculture of Dalian Ocean University:
Respectively purple sea urchin is placed in Strongylocentrotus intermedius kind gallbladder support 3 ~ 10 day temporary with under locality identical temperature environment；Supporting temporarily after terminating, breeding water is progressively heated up, every day, increasing extent of temperature was 0.5 DEG C, Strongylocentrotus intermedius breeding water stops heating up after being warming up to 15 ± 1 DEG C, being maintained at 15 ± 1 DEG C, purple sea urchin breeding water stops heating up after being warming up to 22 ± 1 DEG C, is maintained at 22 ± 1 DEG C；
The shellfish meat that fresh Brown algae throwing something and feeding in breeding process and boils, the time scale thrown something and fed for after three days Brown algaes of often throwing something and feeding, one day shellfish meat of throwing something and feeding, wherein Brown algae is the kelps such as fresh Thallus Laminariae (Thallus Eckloniae) or Thallus Laminariae, shellfish meat is Mytilus edulis meat, clam son's meat, scallop meat or partially pushes up clam meat
Breeding process removes Hemicentrotus seu Strongylocentrotus feces and dead individuals at any time, keeps water quality pure and fresh, within every 1-2 days, change water once, fall weekly pond once, and change water and fall the water temperature temperature difference before and after pond lower than 1 DEG C,
From support temporarily terminate after start at 1 month after, randomly selected purple sea urchin and Strongylocentrotus intermedius 3-5 every 7 days extremely, measure its liveweight, and measure gonad weight in wet base after dissecting, calculate genital gland indices, when genital gland indices reaches more than 20%, it is believed that it is ripe to plant gallbladder,
Kind gallbladder accelerating is hastened parturition in May, 2015 after completing.Its abactinal Spina jujubae, before hastening parturition, is cut short to about 1cm by purple sea urchin kind gallbladder with shears, adopts the method for injection Klorvess Liquid that kind of a gallbladder is hastened parturition, and Klorvess Liquid concentration used is 0.5mol/L, and the injection measurement of every kind gallbladder is 1-2mL,
The anti-actinal surface of female Hemicentrotus seu Strongylocentrotus starting to lay eggs is individually placed in taper bottleneck recovery of ova down that fill sea water, and purple sea urchin ovum collects water temperature 25 ± 1 DEG C；
Desiccation culture ware is laid 1-2 metafiltration paper, the anti-actinal surface of male Strongylocentrotus intermedius starting to produce essence is individually placed in culture dish down, collects sperm, and the temperature of culture dish is 18 ± 1 DEG C, and the sperm collected at the uniform velocity is warming up to 22 ± 1 DEG C in 30min,
Sperm after above-mentioned steps processes is put in the ovum liquid after above-mentioned steps processes, and stirs 1-3 minute, it is thus achieved that germ cell, and the ratio between pure liquid and ovum liquid is 1:1000,
Germ cell being poured in the sea water of 40L, hatching density is 50/mL, hatches water temperature 25 ± 1 DEG C, and what configure 3W in incubation vessel makes wave pump, makes wave pump and is always on, close and make wave pump after blastula stage in the process of development of fertilized ova to blastula stage,
Germling utilizes the bolting silk net of 300 orders to be pulled out by the germling of floating after floating, and is invested in nursery pond and cultivates, and cultivating water temperature is 26 ± 1 DEG C, and culture density is 0.3/mL, and the breeding method of germling is consistent with conventional method for culturing seedlings,
When the average shell Diameter growth of seed is to 1cm, seed is carried out high temperature resistant test, result shows, this kind of hybridization Hemicentrotus seu Strongylocentrotus survival rate under 16-28 DEG C of water temperature raises along with temperature and raises, the speed of growth raises along with temperature and accelerates, and purple sea urchin the most suitable growth water temperature is 24 DEG C, Strongylocentrotus intermedius the most suitable growth water temperature is 16 DEG C.Hybridization Hemicentrotus seu Strongylocentrotus has higher resistance to elevated temperatures compared with its Parent.
Claims (1)
1. the breeding method of a high temperature resistant Hemicentrotus seu Strongylocentrotus, it is characterised in that: described method carries out according to following steps:
A. using female purple sea urchin and male Strongylocentrotus intermedius as kind of a gallbladder, the quality of purple sea urchin is be more than or equal to 60g, and the quality of Strongylocentrotus intermedius, be more than or equal to 50g, is planted gallbladder and required anosis, body surface not damaged,
B. respectively purple sea urchin is placed in male Strongylocentrotus intermedius support 3-10 days temporary with under locality identical water temperature, then breeding water body is progressively heated up, every day, increasing extent of temperature was 0.5 DEG C, the breeding water body water temperature of Strongylocentrotus intermedius stops heating up and being maintained at this temperature after rising to 15 ± 1 DEG C, the breeding water body water temperature of purple sea urchin stops heating up and being maintained at this temperature after rising to 22 ± 1 DEG C
C. purple sea urchin and Strongylocentrotus intermedius is raised using Brown algae and the shellfish meat boiled as bait, the time scale thrown something and fed for after three days Brown algaes of often throwing something and feeding, one day shellfish meat of throwing something and feeding, Brown algae is fresh Thallus Laminariae (Thallus Eckloniae) or Thallus Laminariae, shellfish meat is Mytilus edulis meat, clam son's meat, scallop meat or partially pushes up clam meat
D., breeding process removes Hemicentrotus seu Strongylocentrotus feces and dead individuals at any time, keeps water quality pure and fresh, within every 1-2 days, change water once, fall weekly pond once, and change water and fall the water temperature temperature difference before and after pond lower than 1 DEG C,
E. from support temporarily terminate after start at 1 month after, randomly selected purple sea urchin and Strongylocentrotus intermedius 3-5 every 7 days only, measure liveweight, and measure gonad weight in wet base after dissecting, calculate genital gland indices, when genital gland indices reaches more than 20%, it is believed that it is ripe to plant gallbladder,
Kind of a gallbladder is hastened parturition by the mode f. adopting injection Klorvess Liquid, and the concentration of Klorvess Liquid used is 0.5mol/L, and the injection dosage of every kind gallbladder is 1-2mL,
G. the anti-actinal surface of female purple sea urchin starting to lay eggs is individually placed in down the taper bottle mouth position filling sea water, recovery of ova, and the water temperature collected of ovum be 25 ± 1 DEG C,
H. in desiccation culture ware, lay 1-2 metafiltration paper, the anti-actinal surface of male Strongylocentrotus intermedius starting to produce essence be individually placed in culture dish down, collect sperm, and the temperature of culture dish is 18 ± 1 DEG C, and the sperm collected at the uniform velocity is warming up to 22 ± 1 DEG C in 30min,
I. being put in the ovum liquid after g step process by the sperm after h step process, and stir 1-3 minute, it is thus achieved that germ cell, the ratio between pure seminal fluid and ovum liquid is 1:1000,
J. germ cell being poured in the sea water of 20-40L, hatching density is 50/mL, hatches water temperature 25 ± 1 DEG C, and what configure 1-3W in incubation vessel makes wave pump, makes wave pump and is always on, close and make wave pump after blastula stage in the process of development of fertilized ova to blastula stage,
K. after germling floats, utilizing the bolting silk net of 300 orders to be pulled out by the germling of floating, and be invested in nursery pond and cultivate, cultivating water temperature is 26 ± 1 DEG C, and culture density is 0.2-0.5/mL,
L. the shell Diameter growth of seed is to 1cm, carries out pond culture or suspended longline farming, the high temperature resistant Hemicentrotus seu Strongylocentrotus of final acquisition.
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| CN106259067A (en) * | 2016-08-05 | 2017-01-04 | 山东省海洋资源与环境研究院 | A kind of selection of high temperature resistant Radix Morinae Bulleyanae strain |
| CN106804488A (en) * | 2016-12-29 | 2017-06-09 | 大连海洋大学 | It is adapted to the sea urchin offspring seed cultivation method that northern many algae sea cucumber stable breedings are grown |
| CN106957913A (en) * | 2017-03-28 | 2017-07-18 | 大连海洋大学 | A kind of detection method of the strong vibrios of sea urchin pathogenic bacteria |
| CN107736283A (en) * | 2017-09-22 | 2018-02-27 | 大连海洋大学 | The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin |
| CN109182540A (en) * | 2018-10-10 | 2019-01-11 | 大连海洋大学 | Screen the Strongylocentrotus intermedius primer and method with high-temperature stability |
| CN111109168A (en) * | 2020-03-03 | 2020-05-08 | 大连海洋大学 | Cultivation method of sea urchin Strongylocentrotus intermedius with acidation resistance |
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| CN106259067B (en) * | 2016-08-05 | 2019-03-01 | 山东省海洋资源与环境研究院 | A kind of selection of high temperature resistant stichopus japonicus strain |
| CN106259067A (en) * | 2016-08-05 | 2017-01-04 | 山东省海洋资源与环境研究院 | A kind of selection of high temperature resistant Radix Morinae Bulleyanae strain |
| CN106804488A (en) * | 2016-12-29 | 2017-06-09 | 大连海洋大学 | It is adapted to the sea urchin offspring seed cultivation method that northern many algae sea cucumber stable breedings are grown |
| CN106957913A (en) * | 2017-03-28 | 2017-07-18 | 大连海洋大学 | A kind of detection method of the strong vibrios of sea urchin pathogenic bacteria |
| CN106957913B (en) * | 2017-03-28 | 2020-06-16 | 大连海洋大学 | Detection method of sea urchin pathogenic bacteria robust vibrio |
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| CN107736283A (en) * | 2017-09-22 | 2018-02-27 | 大连海洋大学 | The energy-saving and emission-reduction method for culturing seedlings of blue full dress sea urchin |
| CN109182540A (en) * | 2018-10-10 | 2019-01-11 | 大连海洋大学 | Screen the Strongylocentrotus intermedius primer and method with high-temperature stability |
| CN109182540B (en) * | 2018-10-10 | 2021-06-15 | 大连海洋大学 | Primer and method for screening high-temperature-resistant strongylocentrotus intermedius |
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| CN111109168B (en) * | 2020-03-03 | 2021-07-30 | 大连海洋大学 | Cultivation method of sea urchin Strongylocentrotus intermedius with acidation resistance |
| CN112471014A (en) * | 2020-11-25 | 2021-03-12 | 大连海洋大学 | Method for breeding sea urchin seedlings through medium ball of high-density attached plates |
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