CN107723237B - Multipurpose culture dish for auxiliary reproduction test - Google Patents
Multipurpose culture dish for auxiliary reproduction test Download PDFInfo
- Publication number
- CN107723237B CN107723237B CN201711182159.XA CN201711182159A CN107723237B CN 107723237 B CN107723237 B CN 107723237B CN 201711182159 A CN201711182159 A CN 201711182159A CN 107723237 B CN107723237 B CN 107723237B
- Authority
- CN
- China
- Prior art keywords
- sperm
- ovum
- cup
- placing
- dish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000004681 ovum Anatomy 0.000 claims abstract description 77
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 73
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 73
- 230000000694 effects Effects 0.000 abstract description 16
- 230000009182 swimming Effects 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 235000013601 eggs Nutrition 0.000 description 18
- 210000001161 mammalian embryo Anatomy 0.000 description 7
- 238000000338 in vitro Methods 0.000 description 4
- 230000004720 fertilization Effects 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 238000002054 transplantation Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 238000004115 adherent culture Methods 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Abstract
The invention discloses a multipurpose culture dish for auxiliary reproduction test; comprises a culture dish and a dish cover, wherein the culture dish comprises a dish main body, an ovum placing cup, a plurality of sperm placing cups and a plurality of sperm channels; a columnar supporting table is arranged in the middle part of the dish main body, and an ovum placing cup is fixed on the columnar supporting table; the plurality of sperm placing cups are arranged in the dish main body around the ovum placing cup, the heights of the cup mouths of the plurality of sperm placing cups are slightly lower than the heights of the cup bottoms of the ovum placing cups, the outer walls of the plurality of sperm placing cups are respectively provided with a sperm outlet close to the cup mouths, and the outer walls of the ovum placing cups are provided with a plurality of sperm inlets close to the cup bottoms; the sperm channels are correspondingly fixed between the sperm outlet and the sperm inlet which are opposite. The beneficial effects are that: the sperm channels are all obliquely arranged, so that the winner and the obsolete of sperm swimming can be reflected, and only the sperm with the best activity can swim to the ovum placing cup through the sperm channels to be combined with the high-quality ovum.
Description
Technical Field
The invention relates to the technical field of medical equipment, in particular to a multipurpose culture dish for auxiliary reproduction test.
Background
A culture dish, as its name implies, is a laboratory dish for the cultivation of microorganisms or cells, and a conventional culture dish consists of a disk-shaped dish body and a lid, which is generally made of plastic or glass. Plastics are suitable for laboratory inoculation, streaking and bacterial isolation operations and can also be used for cultivation of plant material. The glass can be used for plant materials, microbial culture and adherent culture of animal cells.
For the traditional in vitro fertilization culture dish, a sperm placing cavity and an ovum placing cavity are generally directly arranged in the corresponding culture dish, and a sperm guiding channel is horizontally connected in the middle of the outer wall between the sperm placing cavity and the ovum placing cavity, so that the active sperm can run to the ovum placing cavity through the corresponding sperm guiding channel to be combined with the ovum placed in the ovum placing cavity, and the purpose of in vitro fertilization of the ovum is achieved.
Specifically, on one hand, the sperm with good activity cannot find the sperm guiding channel, the lost direction still stays in the corresponding sperm placing cavity, but the sperm with poor activity can run to the corresponding sperm placing cavity through the corresponding sperm guiding channel to be combined with the placed sperm in the sperm placing cavity, so that the combination of the sperm with good activity or the sperm with poor activity cannot be ensured to be combined with the sperm with high efficiency, the quality of the bred offspring can be influenced, the development rate of fertilized embryos and the success rate of embryo transplantation are more likely to be reduced, on the other hand, the sperm with good activity is suspended at the inner upper part of the corresponding sperm placing cavity, the high-quality ovum is submerged at the bottom of the corresponding sperm placing cavity, the poor-quality ovum is suspended at the inner upper part of the corresponding ovum placing cavity, and the outlet of the corresponding sperm guiding channel is positioned in the middle of the corresponding ovum placing cavity, so that the combination of the sperm with good activity or the poor-quality is also not ensured to be combined with high efficiency, and the conventional in vitro culture dish with single function cannot be used as an auxiliary fertility test sperm.
Thus, existing in vitro fertilization dishes are in need of improvement.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a multipurpose culture dish for auxiliary reproduction test.
The technical scheme adopted for solving the problems in the prior art is as follows: the multipurpose culture dish comprises a culture dish and a dish cover for covering the culture dish, wherein the culture dish comprises a dish main body, an ovum placing cup, a plurality of sperm placing cups and a plurality of sperm channels;
wherein, a columnar saddle is fixedly arranged in the middle part of the dish main body, and the ovum placing cup is fixedly arranged on the columnar saddle; the plurality of sperm placing cups are uniformly fixed in the dish main body around the ovum placing cup, the heights of the cup mouths of the sperm placing cups are slightly lower than the cup bottom height of the ovum placing cup, the outer walls of the sperm placing cups are respectively provided with a sperm outlet which faces the ovum placing cup near the cup mouths, and the outer walls of the ovum placing cups are provided with a plurality of sperm inlets which face the sperm placing cup at corresponding positions near the cup bottom; the sperm channels are correspondingly and fixedly arranged between the sperm outlet and the sperm inlet which are opposite in position; an ovum filter screen is fixedly arranged at each sperm leading-in port.
The following further describes the above technical scheme:
preferably, the mesh aperture of the ovum filter screen is smaller than the diameter of the ovum and larger than the diameter of the sperm.
Preferably, the cup mouth of the ovum placing cup is lower than the open top of the dish main body.
Preferably, the upper end surface of the columnar support is provided with a fixing groove downwards; the outer wall of the lower end of the ovum placing cup is clamped and fixed in the fixing groove.
Preferably, the top walls of the sperm channels are respectively and uniformly provided with a plurality of vent holes along the length direction.
Preferably, a protection ring which is integrally formed with the dish main body and surrounds the outer wall of the dish main body is fixedly arranged outside the dish main body;
the inner wall of the protection ring and the outer wall of the dish main body form an overflow groove, and the height of the upper edge of the protection ring is higher than the height of the upper end opening of the dish main body.
Preferably, a label groove for attaching the indication label is formed in the outer wall of the protection ring.
Preferably, the outer wall of the protection ring is provided with a plurality of circles of anti-skid lines in a surrounding way.
Preferably, the top walls of the sperm channels are respectively and fixedly provided with a T-shaped switch valve upwards near the sperm guide inlet at the corresponding position.
The beneficial effects of the invention are as follows:
in the implementation, on one hand, the egg placing cups are fixedly arranged in the inner middle part of the dish main body, the plurality of the egg placing cups are uniformly distributed around the egg placing cups, so that the sperms in the plurality of the sperm placing cups can be effectively kept in fair competition and combined with the eggs in the corresponding egg placing cups, the superior and inferior stages of the sperms can be ensured, on the other hand, the sperm channels are all obliquely arranged, the height of the sperm leading-out port is lower than the height of the sperm leading-in port, the superior and inferior stages of the sperms can be reflected more, only the sperms with the best activity can be combined with the eggs through the upstream of the corresponding sperm channel to the corresponding egg placing cup, and combined, the sperms with the good activity are mostly suspended at the upper part in the corresponding sperm placing cup, the high-quality eggs are mostly submerged at the bottom of the corresponding egg placing cup, the sperm leading-out port is arranged on the outer wall of the sperm placing cup and close to the leading-in port, the sperm leading-out port is better in the sperm channel, the high-quality embryo can be achieved, the high-quality sperm leading-out cup is positioned close to the inner cavity of the sperm cup, and the high-quality embryo can be fully transferred, and the high-quality embryo can be achieved, and the success rate of the sperm can be improved, and the high-quality embryo can be achieved, and the high-quality embryo can be finally and fully transferred through the sperm cup.
Secondly, in this technical scheme, on the one hand, the mesh aperture of ovum filter screen is less than the ovum diameter and is greater than the sperm diameter, so, even make it can guarantee that the sperm passes through and separate the ovum and pass through, make avoid ovum loss, on the other hand, the rim of a cup height of ovum placing cup is less than the open height in dish main part upper end, make can avoid the overflow of the culture solution that holds in it, and a plurality of air vents have been seted up respectively along its length direction to the roof of a plurality of sperm passageway evenly, so, then make the culture solution in the sperm passageway be difficult for producing the bubble and hinder the swimming of sperm, and then, the invention uses the reliability strong.
Thirdly, in this embodiment, on the one hand, a protection ring which is integrally formed and surrounds the outer wall of the dish body is fixedly arranged outside the dish body, an overflow groove is formed between the inner wall of the protection ring and the outer wall of the dish body, and the height of the upper edge of the protection ring is higher than the height of the upper end opening of the dish body, so that when excessive overflow occurs in the dish body, the culture solution only overflows to the overflow groove and does not flow out to a workbench to cause pollution, on the other hand, a plurality of T-shaped switching valves are fixedly arranged on the top wall of the sperm channel close to the sperm guiding inlet at the corresponding position, and when the T-shaped switching valves are manually closed, a plurality of sperm channels are closed, so that the egg placing cup and the sperm placing cup are mutually separated, and other auxiliary test culture solutions can be placed in the egg placing cup, and the egg placing cup is used as an auxiliary reproduction culture dish, and the egg placing device has the best overall use effect.
Drawings
FIG. 1 is a schematic view of the overall structure of a multipurpose petri dish for assisted reproduction test according to the present invention;
FIG. 2 is an enlarged view of the connection of one of the sperm placement cups to the egg placement cup via the sperm channel of FIG. 1;
the achievement of the objects, functional features and advantages of the present invention will be further described with reference to the accompanying drawings, in conjunction with the embodiments.
Reference numerals:
a culture dish 10;
a dish body 101; a columnar stand 1011; an ovum placement cup 102; a sperm placement cup 103; sperm channel 104; an ovum filter screen 1041; vent 1042; a protective ring 105; a tag slot 1051; anti-slip texture 1052; an overflow trough 106; t-shaped on-off valve 107.
Detailed Description
The following detailed description of the present invention will be given with reference to the accompanying drawings and specific embodiments, so as to more clearly and intuitively understand the essence of the present invention.
As shown in connection with fig. 1 and 2;
the invention provides a multipurpose culture dish for auxiliary reproduction test, which comprises a culture dish 10 and a dish cover for covering the culture dish 10.
The improvement of the invention is that: the culture dish 10 comprises a dish main body 101, an ovum placing cup 102, a plurality of sperm placing cups 103 and a plurality of sperm channels 104;
wherein, a column-shaped supporting stand 1011 is fixedly arranged in the inner middle part of the dish main body 101, and the ovum placing cup 102 is fixedly arranged on the column-shaped supporting stand 1011; the plurality of sperm placing cups 103 are uniformly fixed in the dish main body 101 around the ovum placing cup 102, the heights of the cup mouths of the sperm placing cups 103 are slightly lower than the cup bottom height of the ovum placing cup 102, the outer walls of the sperm placing cups 103 close to the cup mouths are respectively provided with a sperm outlet facing the ovum placing cup 102, and the outer walls of the ovum placing cups 102 close to the cup bottoms are provided with sperm inlets facing the sperm placing cups 103 corresponding to the corresponding positions; the sperm channels 104 are correspondingly and fixedly arranged between the sperm outlet and the sperm inlet which are opposite; an ovum filter screen 1041 is fixedly arranged at each sperm leading-in port.
Based on the foregoing, it is clear that the multipurpose petri dish 10 for assisted reproduction test according to the present invention, when embodied, is:
on the one hand, the egg placing cup 102 is fixedly arranged in the inner middle part of the dish main body 101, and the plurality of sperm placing cups 103 are uniformly distributed around the egg placing cup 102, so that the sperm in the plurality of sperm placing cups 103 can be effectively kept to fairly compete to be combined with the eggs in the corresponding egg placing cup 102, and the winnings and the obsolescence of the sperm can be ensured.
On the other hand, the heights of the cup openings of the sperm placing cups 103 are slightly lower than the cup bottom height of the ovum placing cup 102, the outer walls of the sperm placing cups 103 close to the cup openings are respectively provided with a sperm guiding opening facing the ovum placing cup 102, and the outer walls of the ovum placing cups 102 close to the cup bottom are provided with a plurality of sperm guiding openings facing the sperm placing cup 103 at corresponding positions; the sperm channels 104 are correspondingly and fixedly arranged between the sperm outlet and the sperm inlet which are opposite; therefore, the heights of the sperm outlets are lower than the heights of the sperm inlets, so that the sperm channels 104 are obliquely arranged, the upward swimming of the sperm can be better reflected, and only the sperm with the best activity can be combined with the ovum through the upstream of the corresponding sperm channel 104 to the corresponding ovum placing cup 102.
In addition, by combining with common knowledge, the active sperms are mostly suspended at the upper part in the corresponding sperm placing cup 103, the high-quality ova mostly sink at the bottom of the corresponding ovum placing cup 102, the sperm outlet is arranged on the outer wall of the sperm placing cup 103 and close to the cup mouth of the sperm placing cup, and the sperm inlet is arranged on the outer wall of the ovum placing cup 102 and close to the cup bottom of the sperm placing cup, so that the sperm channel 104 can preferentially guide the sperm with the best activity to the ovum placing cup 102 along the upper part of the inner cavity of the sperm channel, and finally ensure that the fastest sperm with the best activity and swimming can be combined with the high-quality ova in the ovum placing cup 102, thereby comprehensively ensuring the quality of the propagated offspring and greatly improving the development rate of fertilized embryos and the success rate of embryo transplantation.
Furthermore, the invention has strong practicability and good use effect.
In practice, the diameter of sperm is much smaller than that of an ovum. Therefore, in the present embodiment, the mesh diameter of the ovum filter 1041 is set smaller than the ovum diameter and larger than the sperm diameter.
In this way, it is possible to ensure passage of sperm while blocking passage of ova, so that loss of ova is avoided.
It should be noted that the diameter of the human ovum is about 0.1 mm-0.2 mm, and the length of the human mature sperm is about 60 microns, so that the diameter of the human mature sperm is less than 60 microns (0.06 mm).
Therefore, in the present embodiment, the mesh diameter of the ovum filter screen 1041 is preferably set to be 0.06-0.08 mm.
Preferably, in this technical solution, the mesh diameter of the ovum filter screen 1041 is set to be 0.07 mm.
In this embodiment, the cup mouth of the ovum placement cup 102 is lower than the upper opening of the dish body 101.
In this way, it is possible to avoid the overflow of the culture medium contained therein.
And when in implementation, a fixing groove (not shown) is formed on the upper end surface of the columnar supporting stand 1011 downwards; the lower outer wall of the ovum placement cup 102 is fastened to the fixing groove (not shown).
Preferably, in this embodiment, the top walls of the sperm channels 104 are respectively and uniformly provided with a plurality of ventilation holes 1042 along the length direction thereof.
Thus, the culture medium in the sperm channel 104 is less likely to generate bubbles, which inhibit the migration of sperm.
Furthermore, the invention has strong use reliability.
Further, in the specific implementation, a protecting ring 105 integrally formed with the dish body 101 and surrounding the outer wall of the dish body is fixedly arranged outside the dish body;
the lower edge of the inner wall of the protecting ring 105 is integrally connected with the lower edge of the outer wall of the dish body 101, so that an overflow groove 106 is formed between the inner wall of the protecting ring 105 and the outer wall of the dish body 101, and the upper edge of the protecting ring 105 is higher than the upper opening of the dish body 101.
In this way, when the culture solution in the dish body 101 overflows excessively, the culture solution overflows only to the overflow tank 106 and does not flow out to the workbench to cause pollution,
still further, in the embodiment, a label slot 1051 for attaching an indication label is provided on the outer wall of the protection ring 105.
In this manner, the tab slot 1051 is provided to facilitate on-body labeling to indicate the use of the present invention and the culture broth or the like placed therein, so as to facilitate easy identification at a glance.
Still further, in the embodiment, the outer wall of the protecting ring 105 is provided with a plurality of anti-slip lines 1052.
Thus, when the outer wall of the protecting ring 105 is held by hand, the hands are abutted against the anti-slip lines 1052, so that the anti-slip and anti-slip effects can be effectively achieved.
Further, in the implementation, a T-shaped switch valve 107 is fixed upward to the top wall of the sperm channels 104 near the sperm inlets at the corresponding positions.
Thus, when the plurality of T-shaped switch valves 107 are manually closed, the plurality of sperm passages 104 are closed, so that the ovum placement cup 102 and the plurality of sperm placement cups 103 are separated from each other, and other culture liquids for auxiliary reproduction test can be placed in the ovum placement cup 102 and the plurality of sperm placement cups 103, so that the culture dish 10 for auxiliary reproduction test is used as a culture dish 10, and has multiple uses and comprehensive functions.
Furthermore, the use effect of the invention can be optimized.
It should be noted that, in the implementation, the number of the sperm placement cups 103 is preferably 4, and the 4 sperm placement cups 103 are uniformly distributed on the front, back, left and right sides of the ovum placement cup 102, and in the implementation, two gripping lugs (not shown) are disposed on the outer wall of the protection ring 105 outwards and oppositely, so as to facilitate the holding.
Other embodiments, etc., are not illustrated herein.
In summary, the invention has the advantages of simple integral structure, easy implementation, easy operation, strong practicability, strong specificity and low manufacturing cost, and does not need to increase too much cost in the improvement of the structure and the improvement of the technology, so that the invention has very good market popularization value, and can be very popular and can be effectively popularized.
The foregoing description is only of the preferred embodiments of the present invention, and is not intended to limit the scope of the invention, and all changes in the equivalent structure or equivalent flow, or direct or indirect application in other related technical fields, are included in the scope of the invention.
Claims (7)
1. The utility model provides an auxiliary reproduction test is with multipurpose culture dish, includes the culture dish and is used for the lid to close the dish lid of culture dish, its characterized in that: the culture dish comprises a dish main body, an ovum placing cup, a plurality of sperm placing cups and a plurality of sperm channels;
wherein, a columnar saddle is fixedly arranged in the middle part of the dish main body, and the ovum placing cup is fixedly arranged on the columnar saddle; the plurality of sperm placing cups are uniformly fixed in the dish main body around the ovum placing cup, the heights of the cup mouths of the sperm placing cups are slightly lower than the cup bottom height of the ovum placing cup, the outer walls of the sperm placing cups are respectively provided with a sperm outlet which faces the ovum placing cup near the cup mouths, and the outer walls of the ovum placing cups are provided with a plurality of sperm inlets which face the sperm placing cup at corresponding positions near the cup bottom; the sperm channels are correspondingly and fixedly arranged between the sperm outlet and the sperm inlet which are opposite in position; an ovum filter screen is fixedly arranged at each sperm guide inlet;
the upper end surface of the columnar support is provided with a fixing groove downwards; the outer wall of the lower end of the ovum placing cup is clamped and fixed in the fixing groove;
the top walls of the sperm channels are respectively and uniformly provided with a plurality of vent holes along the length direction.
2. The multipurpose petri dish for assisted reproduction test according to claim 1, wherein: the mesh aperture of the ovum filter screen is smaller than the diameter of the ovum and larger than the diameter of the sperm.
3. The multipurpose petri dish for assisted reproduction test according to claim 1, wherein: the cup mouth of the ovum placing cup is lower than the open top of the dish main body.
4. The multipurpose petri dish for assisted reproduction test according to claim 1, wherein: the outer side of the dish main body is fixedly provided with a protection ring which is integrally formed with the dish main body and surrounds the outer wall of the dish main body;
the inner wall of the protection ring and the outer wall of the dish main body form an overflow groove, and the height of the upper edge of the protection ring is higher than the height of the upper end opening of the dish main body.
5. The multipurpose petri dish for assisted reproduction test according to claim 4, wherein: the outer wall of the protection ring is provided with a label groove for attaching the indication label.
6. The multipurpose petri dish for assisted reproduction test according to claim 4, wherein: the outer wall of the protection ring is surrounded by a plurality of circles of anti-skid lines.
7. The multipurpose petri dish for assisted reproduction test according to any one of claims 1 to 6, wherein: the top walls of the sperm channels are close to the sperm inlets at the corresponding positions, and T-shaped switch valves are respectively and fixedly arranged upwards.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711182159.XA CN107723237B (en) | 2017-11-23 | 2017-11-23 | Multipurpose culture dish for auxiliary reproduction test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711182159.XA CN107723237B (en) | 2017-11-23 | 2017-11-23 | Multipurpose culture dish for auxiliary reproduction test |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107723237A CN107723237A (en) | 2018-02-23 |
| CN107723237B true CN107723237B (en) | 2023-12-08 |
Family
ID=61218176
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711182159.XA Active CN107723237B (en) | 2017-11-23 | 2017-11-23 | Multipurpose culture dish for auxiliary reproduction test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107723237B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109207364A (en) * | 2018-10-11 | 2019-01-15 | 广州洁特生物过滤股份有限公司 | A kind of cell culture apparatus |
| CN109251860A (en) * | 2018-10-17 | 2019-01-22 | 北京大学深圳医院 | Reproductive center laboratory embryo breeding utensil |
| CN109609358A (en) * | 2019-01-29 | 2019-04-12 | 浙江大学 | A kind of the micromanipulation ware and sperm preferred method of preferred sperm |
Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5512476A (en) * | 1991-05-14 | 1996-04-30 | Mount Sinai School Of Medicine Of The City University Of New York | Method for in vitro fertilization of oocytes using microchambers |
| US5691194A (en) * | 1991-05-14 | 1997-11-25 | Mount Sinai School Of Medicine Of The City University Of New York | Method and apparatus for in vitro fertilization |
| KR20050014087A (en) * | 2003-07-30 | 2005-02-07 | 재단법인서울대학교산학협력재단 | Methods of In Vitro Fertilization Showing Reduced Polyspermy and Tubes therefor |
| CN201343531Y (en) * | 2008-12-17 | 2009-11-11 | 陈静 | Test tube rack capable of facilitating sperm to swim upward or downward |
| JP2009273385A (en) * | 2008-05-13 | 2009-11-26 | Nipro Corp | Petri dish for in vitro fertilization and method for in vitro fertilization |
| WO2012019436A1 (en) * | 2010-08-10 | 2012-02-16 | Capitalbio Corporation | Microfluidic device for cell motility screening and chemotaxis testing |
| CN202322835U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | In vitro fertilization culture dish for optimizing sperms |
| WO2013110237A1 (en) * | 2012-01-27 | 2013-08-01 | Peng Jianjun | Culture dish |
| CN204342767U (en) * | 2014-12-02 | 2015-05-20 | 张丽红 | Cultivation sieve in vitro fertilization |
| CN105154320A (en) * | 2015-10-12 | 2015-12-16 | 深圳市博锐德生物科技有限公司 | Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device |
| JP2016007179A (en) * | 2014-06-25 | 2016-01-18 | 大日本印刷株式会社 | Cell culture vessel |
| CN205603602U (en) * | 2016-01-28 | 2016-09-28 | 北京大学深圳医院 | Embryo culture dish |
| CN206486508U (en) * | 2017-02-13 | 2017-09-12 | 瑞柏生物（中国）股份有限公司 | A kind of passage fertilization culture dish |
| CN206486509U (en) * | 2017-02-13 | 2017-09-12 | 瑞柏生物（中国）股份有限公司 | A kind of high-quality sperm screening injection ware |
| CN207525253U (en) * | 2017-11-23 | 2018-06-22 | 北京大学深圳医院 | Supplementary reproduction experiment multipurpose culture dish |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6929945B2 (en) * | 2002-12-09 | 2005-08-16 | Advanced Fluidix Laboratories Llc | Male fertility assay method and device |
| EP1655363A1 (en) * | 2003-06-20 | 2006-05-10 | Nitto Denko Corporation | Cell microchip |
| TW201040524A (en) * | 2009-05-15 | 2010-11-16 | Nat Univ Tsing Hua | Method of using microfluidic chip to sort high motility sperm |
| JP2018512116A (en) * | 2015-03-04 | 2018-05-17 | バークレー ライツ，インコーポレイテッド | In vitro embryo generation and selection |
| CN206319014U (en) * | 2016-12-30 | 2017-07-11 | 四川启域科技咨询有限公司 | A kind of Embryo Culture Multifunctional petri dish |
-
2017
- 2017-11-23 CN CN201711182159.XA patent/CN107723237B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5512476A (en) * | 1991-05-14 | 1996-04-30 | Mount Sinai School Of Medicine Of The City University Of New York | Method for in vitro fertilization of oocytes using microchambers |
| US5691194A (en) * | 1991-05-14 | 1997-11-25 | Mount Sinai School Of Medicine Of The City University Of New York | Method and apparatus for in vitro fertilization |
| KR20050014087A (en) * | 2003-07-30 | 2005-02-07 | 재단법인서울대학교산학협력재단 | Methods of In Vitro Fertilization Showing Reduced Polyspermy and Tubes therefor |
| JP2009273385A (en) * | 2008-05-13 | 2009-11-26 | Nipro Corp | Petri dish for in vitro fertilization and method for in vitro fertilization |
| CN201343531Y (en) * | 2008-12-17 | 2009-11-11 | 陈静 | Test tube rack capable of facilitating sperm to swim upward or downward |
| WO2012019436A1 (en) * | 2010-08-10 | 2012-02-16 | Capitalbio Corporation | Microfluidic device for cell motility screening and chemotaxis testing |
| CN202322835U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | In vitro fertilization culture dish for optimizing sperms |
| WO2013110237A1 (en) * | 2012-01-27 | 2013-08-01 | Peng Jianjun | Culture dish |
| JP2016007179A (en) * | 2014-06-25 | 2016-01-18 | 大日本印刷株式会社 | Cell culture vessel |
| CN204342767U (en) * | 2014-12-02 | 2015-05-20 | 张丽红 | Cultivation sieve in vitro fertilization |
| CN105154320A (en) * | 2015-10-12 | 2015-12-16 | 深圳市博锐德生物科技有限公司 | Device for selecting sperms coated with hyaluronic acid and hyaluronates or hyaluronic acid derivatives, and application of device |
| CN205603602U (en) * | 2016-01-28 | 2016-09-28 | 北京大学深圳医院 | Embryo culture dish |
| CN206486508U (en) * | 2017-02-13 | 2017-09-12 | 瑞柏生物（中国）股份有限公司 | A kind of passage fertilization culture dish |
| CN206486509U (en) * | 2017-02-13 | 2017-09-12 | 瑞柏生物（中国）股份有限公司 | A kind of high-quality sperm screening injection ware |
| CN207525253U (en) * | 2017-11-23 | 2018-06-22 | 北京大学深圳医院 | Supplementary reproduction experiment multipurpose culture dish |
Non-Patent Citations (6)
| Title |
|---|
| .Rheotaxis facilitates upstream navigation of mammalian sperm cells.《ELIFE》.2014,第03卷第1-12页. * |
| Fertilization signalling and protein-tyrosine kinases;Ken-ichi Sato 等;;《Comparati6e Biochemistry and Physiology》;第126卷(第02期);第129-148页 * |
| Ken-ichi Sato 等 * |
| Vasily Kantsler 等 * |
| 用于***筛选及体外受精的微流控芯片的构建及应用;谢兰;《中国优秀博硕士论文全文数据库（博士） 医药卫生科技辑》》(第06期);第E068-4页 * |
| 短时受精后受精率高与受精完全失败后早补救患者的临床结局和***参数分析;周亮 等;《中国男科学杂志》;第28卷(第10期);第26-30页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107723237A (en) | 2018-02-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107723237B (en) | Multipurpose culture dish for auxiliary reproduction test | |
| JP6349847B2 (en) | Cell culture vessel | |
| CN214088530U (en) | Culture dish | |
| CN111793562A (en) | Culture dish and application thereof | |
| CN202482319U (en) | External fertilization culture dish | |
| CN201469382U (en) | Culture dish for in vitro fertilization | |
| CN207525253U (en) | Supplementary reproduction experiment multipurpose culture dish | |
| CN206418141U (en) | A kind of Embryo Culture ware | |
| CN215953147U (en) | In-vitro incubation device for in-vivo brain slice/tissue slice | |
| CN115851435A (en) | Constant temperature equipment with partition multislot temperature regulation structure | |
| EP1970433B1 (en) | An algae microbe photosynthesis reaction system and method thereof | |
| CN211921518U (en) | Liquid exchanger for cell culture | |
| CN212077079U (en) | Cell culture bottle | |
| CN203788929U (en) | Separating device for separating eggs and larva of brine shrimps | |
| CN209359113U (en) | A kind of rose planting unit that can be poured up and down | |
| CN212212037U (en) | Carbon dioxide supply device for vegetable planting incubator | |
| CN211471441U (en) | Nursing apparatus for assisting reproduction | |
| CN204342767U (en) | Cultivation sieve in vitro fertilization | |
| CN213153343U (en) | Batch appraisal wheat seedling stage salt tolerance device | |
| CN215440490U (en) | Embryo operation and culture dish | |
| CN216982975U (en) | Soilless planting device for motherwort | |
| CN214593209U (en) | Plant disease-resistant identification culture observation box | |
| CN220056816U (en) | Culture dish | |
| CN201168020Y (en) | External fertilization dish | |
| CN219628552U (en) | Agricultural seedling tray |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |