CN215953147U - In-vitro incubation device for in-vivo brain slice/tissue slice - Google Patents
In-vitro incubation device for in-vivo brain slice/tissue slice Download PDFInfo
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- CN215953147U CN215953147U CN202122268477.6U CN202122268477U CN215953147U CN 215953147 U CN215953147 U CN 215953147U CN 202122268477 U CN202122268477 U CN 202122268477U CN 215953147 U CN215953147 U CN 215953147U
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Abstract
The utility model discloses an in-vitro incubation device for a live brain slice/tissue slice, which comprises an incubation platform, an incubation container and an incubation groove, wherein the incubation platform is provided with a first incubation groove and a second incubation groove; the incubation platform comprises an incubation platform base, an incubation platform back plate, a plurality of water tank type electric heating constant-temperature water baths and an air duct device, wherein the water tank type electric heating constant-temperature water baths are arranged above the incubation platform, and the air duct device is arranged on the incubation platform back plate and is used for ventilating incubation liquid in an incubation container in the water tank type electric heating constant-temperature water baths; the incubation container is fixed on the water tank type electric heating constant-temperature water bath kettle and is used for containing incubation liquid; the incubation groove is matched with the incubation container and used for placing the slices, and the incubation groove is used for ensuring that the slices are completely soaked in the incubation liquid. The utility model is beneficial to simultaneously executing the section incubation under multiple conditions, can be convenient for experimenters to improve the working efficiency, reduces the trouble caused by the lack of multiple incubation hardware conditions, and completes the incubation in a one-stop way so as to better perform the follow-up research.
Description
Technical Field
The utility model relates to the technical field of animal in-vitro experimental equipment, in particular to an in-vitro incubation device for a living brain slice/tissue slice.
Background
In animal in vitro experiments, incubation or pharmacological studies of brain slices/tissue sections are often involved. The cut brain slices/tissue sections need to be placed in nutrient solution, incubation solution or drug solution, which has certain pH value, osmotic pressure and concentration, maintains certain temperature and gives ventilation under certain conditions, and fully acts with the slices for certain time. In order to achieve the test conditions, the brain slice/tissue slice incubation groove becomes an indispensable single product, and can be widely applied to patch clamp and other experiments of electrophysiology, immunology, histochemistry and the like to incubate various brain slice/tissue slice specimens.
The following experimental conditions are often involved in brain/tissue slice incubation studies: (1) incubation under different mixed gas conditions, such as oxygen deficiency, high pressure oxygen, and the like; (2) incubation at different pH, osmolality or concentration; (3) incubation under different temperature conditions; (4) different incubation times; (5) incubating different tissue parts simultaneously, such as hippocampal brain slice, cortical brain slice, etc.; (6) incubating in dark; (7) expensive drug incubation, incubation of fine tissue sections such as spinal cord discs, dorsal root ganglia, and the like. At present, most of commercial brain slice/tissue slice incubation grooves (such as Warner company in America) in the market are designed as a single groove, only one incubation cup is provided, and if synchronous incubation of a plurality of tissue section slices under the same condition or different conditions is to be realized, a plurality of incubation grooves are required to be prepared; furthermore, the non-permeability of the incubation cup prevents the tissue section from being in sufficient contact with the incubation liquid. There are also many labs can pass through self-contained incubation devices such as beaker, water bath, breather pipe, incubation vat, but the condition of hatching can appear in a busy or disorderly condition, artificial incubation condition nonconformity that causes and press from both sides the structural damage that leads to when getting the section when hatching the condition many.
SUMMERY OF THE UTILITY MODEL
The utility model aims to overcome the defects of the prior art and provides an in-vitro incubation device for a live brain slice/tissue slice, which can realize synchronous incubation under a plurality of conditions including gas perfusion, incubation liquid, temperature, time and the like so as to complete the incubation research of the brain slice/tissue slice more scientifically and effectively.
In order to achieve the purpose, the utility model adopts the following technical scheme:
an in vitro incubation device for brain slices/tissue slices of a living body comprises an incubation platform, an incubation container and an incubation groove;
the incubation platform comprises an incubation platform base, an incubation platform back plate, a plurality of water tank type electric heating constant-temperature water baths and an air duct device, wherein the water tank type electric heating constant-temperature water baths are arranged above the incubation platform, and the air duct device is arranged on the incubation platform back plate and is used for ventilating incubation liquid in an incubation container in the water tank type electric heating constant-temperature water baths;
the incubation container is fixed on the water tank type electric heating constant-temperature water bath kettle and is used for containing incubation liquid;
the incubation groove is matched with the incubation container and used for placing the slices, and the incubation groove is used for ensuring that the slices are completely soaked in the incubation liquid.
Further, basin formula electric heat thermostat water bath is the cuboid structure, and it includes inner bag and shell, the inner bag adopts stainless steel material, and the shell adopts cold-rolled sheet plastic blasting to form, and inner bag and shell intermediate layer are with the cotton adiabatic of glass, and the inner bag bottom is equipped with copper electrothermal tube and bracket, has wire connection temperature controller.
Further, the incubation platform base and the incubation platform back plate are both made of cold-rolled sheet panels and can absorb a magnetic timer, a constant-temperature digital control regulator is arranged in the incubation platform base and is connected with the copper electric heating tube through a wire.
Furthermore, the front of the water tank type electric heating constant temperature water bath kettle is embedded with an erasable and writable white board, the outer side of the bottom of the water tank type electric heating constant temperature water bath kettle is provided with a water drain valve, and the two sides of the top of the water tank type electric heating constant temperature water bath kettle are provided with inserting grooves.
Further, the ventilation pipeline device comprises a flow rate regulator, an adjustable three-way pipe, a ventilation pipe and a sand filtering porous stone, wherein the ventilation pipe is fixed on the back plate through a clamping groove, the flow rate regulator is used for regulating the flow rate of gas in the ventilation pipe, and the sand filtering porous stone is arranged at the bottom of the ventilation pipe.
Further, the incubation container comprises a large container and a small container, wherein the large container and the small container are made of polystyrene plastic materials, and if the large container and the small container need to be protected from light, the large container and the small container are made of black light-proof materials.
Furthermore, the large container is divided into a plurality of compartments by partition plates, scales are arranged in every other compartment, numbers are sequentially marked on the outer side wall of the large container to mark each compartment, an upper concave small clamping groove and a lower concave small clamping groove are arranged on the inner side surface of every other compartment close to the partition plates and used for fixing the vent pipe, and a hanging lug is arranged on the inner side of the large container.
Furthermore, the small container is composed of a row of a plurality of cylindrical small cups, the inner wall of the small container is provided with scales and a small clamping groove for fixing the small ventilation tube, the outer side wall of the small container is sequentially marked with numbers to mark each incubation cup, and two sides of the small container are provided with hangers.
Further, the incubation groove includes big incubation groove and little incubation groove, big incubation groove is single couple discoid, and couple length is adjustable, and its bottom is equipped with the screen cloth, big incubation inslot activity sets up the adjustable spacer of "ten" font, the adjustable spacer of "ten" font separates angularly adjustable, can imbed fan-shaped microgroove in the adjustable spacer of "ten" font divided compartment.
Further, the small incubation groove is in a shape of a double-ear-hanging disc and can be hung in each incubation cup of the small container, and a cross-shaped partition plate and a fan-shaped small groove can also be inserted into the small incubation groove.
Compared with the prior art, the utility model at least comprises the following beneficial effects:
the living brain slice/tissue slice incubation groove device is beneficial to simultaneously executing slice incubation under multiple conditions, can be convenient for experimenters to improve the working efficiency, reduces the trouble caused by the lack of various incubation hardware conditions, and completes incubation in a one-stop way so as to better perform subsequent research.
Drawings
FIG. 1 is a schematic diagram of the front structure of the incubation platform of the present invention;
FIG. 2 is a schematic front view of a large container and a small container according to the present invention;
FIG. 3 is a schematic view of the structure of a large incubation groove and a small incubation groove of the present invention;
FIG. 4 is a schematic view of a cross-shaped adjustable separator of the present invention;
FIG. 5 is a schematic view of an embedded fan-shaped cell according to the present invention;
in the figure: 1-water tank type electric heating constant temperature water bath, 1.1-bracket, 1.2-copper electric heating tube, 1.3-water drain valve, 1.4-erasable and writable white board, 1.5-slot;
2-incubation platform base, 2.1-constant temperature digital control regulator;
3-incubation platform backboard, 3.1-incubation platform power line jack;
4-ventilating pipe device, 4.1-flow rate regulator, 4.2-adjustable three-way pipe, 4.3-clamping groove, 4.4-ventilating pipe and 4.5-sand filtering porous stone;
5-incubation container, 5.1-large container, 5.2-small container, 5.3-clapboard, 5.4-hanging lug and 5.5-hanging hook;
6-incubation groove, 6.1-big incubation groove, 6.2-small incubation groove, 6.3-cross-shaped adjustable separation sheet and 6.4-fan-shaped small groove.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It is to be noted that the experimental methods described in the following embodiments are all conventional methods unless otherwise specified, and the reagents and materials, if not otherwise specified, are commercially available; in the description of the present invention, the terms "lateral", "longitudinal", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Furthermore, the terms "horizontal", "vertical", "overhang" and the like do not imply that the components are required to be absolutely horizontal or overhang, but may be slightly inclined. For example, "horizontal" merely means that the direction is more horizontal than "vertical" and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.
In the description of the present application, it is further noted that, unless expressly stated or limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
As shown in fig. 1-5, the main body of the incubation platform is composed of two water tank type electric heating constant temperature water bath pots 1, the main body is of a rectangular structure, the inner container is made of stainless steel materials, the outer shell is made of cold-rolled sheet by plastic spraying, and the interlayer of the inner container and the outer shell is insulated by glass wool, so that the heating is fast and electricity is saved. The bottom of the inner container is provided with a copper electric heating tube 1.2 and a bracket 1.1 which are connected with a constant temperature digital control regulator 2.1 by a lead. The front surface of the water tank type electric heating constant temperature water bath kettle 1 is embedded with an erasable and writable white board 1.4, the platform back board is fixed with a ventilation pipeline device 4, the flow of the introduced gas can be adjusted, and the diameter of the ventilation pipe 4.4 can be adjusted according to the requirement. Incubation platform base 2 is equipped with constant temperature digital control regulator 2.1, and incubation platform base 2 and incubation platform backplate 3 all adopt cold-rolled sheet panel, adsorbable magnetic timer, can set up incubation temperature and time according to experimental needs. The water drain valves 1.3 are arranged on the outer sides of the two water baths, and water can be emptied when ice cubes are needed for incubation. Be equipped with on hatching platform backplate 3 and hatch platform power cord jack 3.1 to be connected with external power supply and provide the electric energy for hatching the device. The ventilation pipeline device 4 is arranged on the back plate of the incubation platform and ventilates the incubation liquid in the incubation container 5 in the water tank type electric heating constant temperature water bath 1.
In a further preferred embodiment, the vent pipe device 4 comprises a flow rate regulator 4.1, an adjustable three-way pipe 4.2, a vent pipe 4.4 and a sand filtering porous stone 4.5, wherein the vent pipe 4.4 is fixed on the back plate through a clamping groove 4.3, the flow rate regulator 4.1 is used for regulating the gas flow rate in the vent pipe 4.4, and the sand filtering porous stone 4.5 is arranged at the bottom of the vent pipe 4.4.
The incubation container 5 can be divided into a large container 5.1 and a small container 5.2 according to the volume of the incubation liquid, is in a hanging ear type and can be fixed in slots 1.5 at two sides of the water bath kettle to prevent the side turning over due to floating. The big container and the small container are made of polystyrene plastics, and if the big container and the small container need to be protected from light, the big container and the small container are made of black opaque materials. The large container 5.1 can be divided into four partitions by the partition board 5.3, and can be used for slice incubation under four different conditions at the same temperature or sequential incubation under four different conditions of the same batch of slices, wherein the incubation liquid can be contained to 300 ml at intervals, scales are arranged in the incubation liquid, and the outer side wall is sequentially marked with numbers 1-4 to mark the intervals. An upper concave small clamping groove and a lower concave small clamping groove 4.3 are arranged at the positions of every other inner side surface, which are close to the partition plates 5.3, and the vent pipe can be embedded from the notch and used for fixing the vent pipe 4.4; the small container 5.2 comprises four cylindrical small cups in a row, can hold 30 ml incubation liquid at most, and the inner wall is equipped with scale and little draw-in groove 4.3 and is used for the fixed tubule of ventilating, marks number 1 ~ 4 in proper order on the lateral wall and incubate the cup in order to the sign every, also can realize multiple incubation condition with the same principle. Both sides of the large container and the small container are provided with hangers 5.4, and the side wall of the incubation container 5 is provided with a hook 5.5 for fixing the incubation groove 6. In addition, the large and small containers are provided with corresponding cover plates at intervals, and the cover plates can keep warm, keep out of the sun or prevent dust from falling (the cover plates are not drawn on the drawing).
The incubation well 6 is matched with the incubation container 5 and is also divided into a large incubation well 6.1 and a small incubation well 6.2. Big incubation groove 6.1 is single couple of formula discoid, can hang on 5.1 every inside walls of big container, conveniently takes off in order to change to another incubation condition, according to the adjustable big incubation groove handle length of incubation liquid measure. The bottom of the incubation groove is designed by adopting a screen to ensure that the slices are completely soaked in the incubation liquid, and the periphery of the incubation groove is surrounded by plastic materials with the height of 2 cm. If tissue sections of different sources or types need to be incubated simultaneously, cross-shaped adjustable separation sheets 6.3 can be added into the incubation groove to divide the incubation groove into four small partitions, fan-shaped small grooves 6.4 are embedded into every other small groove, the side walls of the small grooves are provided with capitalized English letter numbers, and the small grooves can be clamped by tweezers to change incubation conditions. The large incubation well 6.1 is equipped with a small lid (not shown in the figure) which can be used to protect from light or to prevent the section from drifting out; the small incubation groove 6.2 is in a shape of a double-ear-hanging disc and can be hung and embedded in each incubation cup of the small container 5.2, and a cross-shaped partition plate and a fan-shaped small groove can also be inserted into the small incubation groove.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention and do not limit the spirit and scope of the present invention. Various modifications and improvements of the technical solutions of the present invention may be made by those skilled in the art without departing from the design concept of the present invention, and the technical contents of the present invention are all described in the claims.
Claims (10)
1. An in vitro incubation device for brain slices/tissue slices of a living body is characterized by comprising an incubation platform, an incubation container and an incubation groove;
the incubation platform comprises an incubation platform base, an incubation platform back plate, a plurality of water tank type electric heating constant-temperature water baths and an air duct device, wherein the water tank type electric heating constant-temperature water baths are arranged above the incubation platform, and the air duct device is arranged on the incubation platform back plate and is used for ventilating incubation liquid in an incubation container in the water tank type electric heating constant-temperature water baths;
the incubation container is fixed on the water tank type electric heating constant-temperature water bath kettle and is used for containing incubation liquid;
the incubation groove is matched with the incubation container and used for placing the slices, and the incubation groove is used for ensuring that the slices are completely soaked in the incubation liquid.
2. The in vitro incubation device for the brain slice/tissue slice of the living body of claim 1, wherein the water tank type electric heating constant temperature water bath kettle is of a cuboid structure and comprises an inner container and a shell, the inner container is made of stainless steel materials, the shell is formed by spraying cold rolled sheets, an interlayer between the inner container and the shell is insulated by glass wool, a copper electric heating tube and a bracket are arranged at the bottom of the inner container, and a wire is connected with a temperature controller.
3. The in vitro incubation device for live brain slices/tissue slices of claim 2, wherein the incubation platform base and the incubation platform back plate are cold rolled plates and can absorb a magnetic timer, and a constant temperature digital control regulator is arranged in the incubation platform base and is connected with the copper electric heating tube through a wire.
4. The in vitro incubation device for the brain slice/tissue slice of the living body of claim 1, wherein the front surface of the water tank type electric heating thermostat water bath kettle is embedded with an erasable and writable white board, the outer side of the bottom is provided with a water drain valve, and the two sides of the top are provided with slots.
5. An in vitro incubation device for brain slices/tissue slices of a living body according to claim 1, wherein the ventilation pipe means comprises a flow rate regulator, an adjustable tee, a ventilation pipe fixed on the back plate by a clamping groove, and a sand filter porous stone for regulating the flow rate of gas in the ventilation pipe, wherein the sand filter porous stone is arranged at the bottom of the ventilation pipe.
6. The in vitro incubation device for the brain slice/tissue slice of the living body of claim 1, wherein the incubation container comprises a large container and a small container, the large container and the small container are made of polystyrene plastic, and if the large container and the small container need to be protected from light, the large container and the small container are made of black opaque materials.
7. The in vitro incubation device according to claim 6, wherein the large container is divided into a plurality of compartments by partitions, scales are arranged in every other compartment, numbers are sequentially marked on the outer side wall of the large container to identify each compartment, an upper concave small clamping groove and a lower concave small clamping groove are arranged on the inner side surface of every other compartment close to the partitions for fixing the ventilating pipes, and the inner side of the large container is provided with a hanging lug.
8. The in vitro incubation device according to claim 6, wherein the small container comprises a row of a plurality of cylindrical small cups, the inner wall of the small container is provided with scales and small slots for fixing the small ventilation tubes, the outer side wall of the small container is sequentially marked with numbers to identify each incubation cup, and the small container is provided with lugs at two sides.
9. The in vitro incubation device for brain/tissue slices of a living body of claim 1, wherein the incubation groove comprises a large incubation groove and a small incubation groove, the large incubation groove is in a shape of a single hook disc, the length of the hook is adjustable, a screen is arranged at the bottom of the large incubation groove, a cross-shaped adjustable partition plate is movably arranged in the large incubation groove, the separation angle of the cross-shaped adjustable partition plate is adjustable, and a fan-shaped small groove can be embedded in a compartment separated by the cross-shaped adjustable partition plate.
10. An in vitro incubation device for brain/tissue slices of a living body according to claim 9, wherein the small incubation groove is a double-ear disc shape, which can be hung in each incubation cup of a small container, wherein a cross-shaped partition plate and a fan-shaped small groove can also be inserted.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122268477.6U CN215953147U (en) | 2021-09-18 | 2021-09-18 | In-vitro incubation device for in-vivo brain slice/tissue slice |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122268477.6U CN215953147U (en) | 2021-09-18 | 2021-09-18 | In-vitro incubation device for in-vivo brain slice/tissue slice |
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| CN215953147U true CN215953147U (en) | 2022-03-04 |
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| CN202122268477.6U Active CN215953147U (en) | 2021-09-18 | 2021-09-18 | In-vitro incubation device for in-vivo brain slice/tissue slice |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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2021
- 2021-09-18 CN CN202122268477.6U patent/CN215953147U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114252324A (en) * | 2022-03-02 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
| CN114252324B (en) * | 2022-03-02 | 2022-05-13 | 中国人民解放军军事科学院军事医学研究院 | Device is hatched to porous brain piece that facilitates use |
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