CN212077079U

CN212077079U - Cell culture bottle

Info

Publication number: CN212077079U
Application number: CN201922394125.8U
Authority: CN
Inventors: 陈廷涛
Original assignee: Nanchang University
Current assignee: Nanchang University
Priority date: 2019-12-27
Filing date: 2019-12-27
Publication date: 2020-12-04
Anticipated expiration: 2029-12-27

Abstract

The utility model discloses a cell culture bottle, including bottle and cork, be provided with the cavity in the bottle, the bottom evenly is provided with a plurality of cell culture boards in the bottle, the inside cavity of cell culture board, cell culture board bottom and cavity intercommunication, bottle upper portion are provided with two bottlenecks, bottleneck and cork cooperate, are provided with permeability cell and pipette on the cork of left side, are provided with on the cork of right side and annotate liquid pipe and permeability cell, and this cell culture bottle can effectively solve the cell culture process and receive the limited problem of pollution, single cell culture bottle culture cell volume easily, creates comfortable ambient temperature for the cell of cultivateing.

Description

Cell culture bottle

Technical Field

The utility model relates to a cell culture equipment especially relates to a cell culture bottle.

Background

Cell culture refers to a method of simulating in vivo environment (sterile, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain the main structure and function.

The cell culture bottle in the prior art has a simple structure and cannot create comfortable environment temperature for cultured cells. In the process of culture solution addition and waste liquid discharge, the container cover is usually required to be opened, so that the container cover is easily influenced by the external environment, the cell culture operation is failed, cells can only grow on the inner wall of the cell culture bottle in the cell culture bottle, the amount of the cells cultured in a single cell culture bottle at one time is limited, and a large number of cell culture bottles are required to be used for culturing mammalian cells, and the mammalian cell culture is not facilitated.

SUMMERY OF THE UTILITY MODEL

The utility model discloses aim at providing a cell culture bottle to prior art's technical defect, can effectively solve the cell culture process and receive easily and pollute, the limited problem of single cell culture bottle culture cell volume, create comfortable ambient temperature for the cell of cultivateing.

In order to achieve the above object, the utility model provides a following technical scheme:

a cell culture bottle comprises a bottle body and a cork, wherein a cavity is arranged in the bottle body, a drain pipe is arranged at the bottom of the bottle body, a drain valve is arranged on the drain pipe, a water inlet pipe is arranged at the upper left part of the bottle body, a water inlet valve is arranged on the water inlet pipe, a plurality of cell culture plates are uniformly arranged at the bottom in the bottle body, the cell culture plates are hollow, the bottoms of the cell culture plates are communicated with the cavity, a heat preservation layer is arranged at the outer side of the bottle body, two bottle openings are arranged at the upper part of the bottle body, the bottle openings are matched with the cork, a vent pipe and a pipette are arranged on the cork plug at the left side, the vent pipe is positioned at the left side of the pipette, a liquid injection pipe and a vent pipe are arranged on the left side of the vent pipe, the upper portion of the liquid suction pipe is provided with a small water pump, the bottom of the liquid injection pipe is communicated with the inside of the bottle body, the upper portion of the liquid injection pipe is provided with a liquid injection valve, and the top of the liquid injection pipe is fixedly connected with a conical opening.

In this embodiment: the material of the heat insulation layer is polyurethane foam.

In this embodiment: the vent pipe, the pipette, the liquid injection pipe and the cork are detachably connected.

The utility model has the advantages that: (1) the inner surface of the bottle body and the outer surface of the cell culture plate are subjected to modification treatment, so that the cell attachment growth is supported, and the culture area is increased; (2) cell fluid and culture fluid are injected through the liquid injection pipe, and the hydrophobic breathable film is used for ventilation, so that the bottle cap is not opened, and external bacterial pollution is prevented; (3) after the cells grow for a certain time, the waste liquid is extracted through the pipette, and the cells attached to the inner surface of the bottle body and the outer surface of the cell culture plate are not easy to fall off; (4) when the temperature is higher, cold water is injected into the cavity through the water inlet pipe, and when the temperature is lower, warm water is injected into the cavity through the water inlet pipe, so that a comfortable temperature is created for cell propagation.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a top view of FIG. 1A-A;

reference numerals: the device comprises a bottle body 1, a cell culture plate 2, a cavity 3, a heat preservation layer 4, a pipette 5, a small water pump 6, a hydrophobic breathable film 7, a breathable pipe 8, a liquid injection pipe 9, a liquid injection valve 10, a conical opening 11, a cork 12, a water inlet pipe 13, a water inlet valve 14, a water outlet pipe 15, a water outlet valve 16 and a bottle opening 17.

Detailed Description

The present invention will be described in detail with reference to the accompanying drawings, as shown in fig. 1-2, a cell culture bottle comprises a bottle body 1 and a cork 12, a cavity 3 is provided in the bottle body 1, a drain pipe 15 is provided at the bottom of the bottle body 1, a drain valve 16 is provided on the drain pipe 15, a water inlet pipe 13 is provided at the upper left portion of the bottle body 1, a water inlet valve 14 is provided on the water inlet pipe 13, and the water inlet pipe 13 and the drain pipe 15 facilitate the replacement of liquid in the cavity 3 and the control of the volume of liquid in the cavity 3.

In this embodiment: the bottom evenly is provided with a plurality of cell culture boards 2 in bottle 1, the inside cavity of

cell culture board

2, 2 bottoms and the 3 intercommunications of cavity of cell culture board, and 1 internal surface of bottle and 2 external surfaces of cell culture board have all made modification treatment, and the cell can adhere to the wall, divide and grow.

In this embodiment: the outer side of the bottle body 1 is provided with a heat preservation layer 4, the upper part of the bottle body 1 is provided with two bottle openings 17, the cork 12 is matched with the bottle openings 17, the cork 12 on the left side is provided with a vent pipe 8 and a pipette 5, the vent pipe 8 is positioned on the left side of the pipette 5, the cork 12 on the right side is provided with an injection pipe 9 and a vent pipe 8, the injection pipe 9 is positioned on the left side of the vent pipe 8, the vent pipe 8 is provided with a hydrophobic breathable film 7, one end of the vent pipe 8 is communicated with the atmosphere, the other end is communicated with the inside of the bottle body 1, oxygen and carbon dioxide are conveniently exchanged with the outside, the lower part of the pipette 5 is directly communicated with the bottom of the bottle body 1, the upper part of the pipette 5 is provided with a small water pump 6 which can absorb waste liquid in a culture bottle, the bottom of the injection pipe 9, is not easy to spill outside and pollutes the laboratory environment.

In this embodiment: the heat-insulating layer 4 is made of polyurethane foam and has a good heat-insulating effect.

In this embodiment: the vent pipe 8, the pipette 5, the liquid injection pipe 9 and the cork 12 are detachably connected, so that the later-stage regular cleaning, sterilization and disinfection are facilitated.

The utility model discloses a theory of operation is: the internal surface of bottle 1 and the external surface of cell culture board 2 are through the modification treatment, support cell adhesion growth, the culture area has been increased, pour into cell sap and culture solution through annotating liquid pipe 9, take a breath through hydrophobic ventilated membrane 7, need not open the bottle lid, prevent external bacterial contamination, after the cell growth certain time, extract the waste liquid through pipette 5, the cell of attaching to internal surface of bottle 1 and the external surface of cell culture board 2 is difficult to drop, when temperature is higher, pour into cavity 3 with cold water through inlet tube 13, when temperature is on the low side, pour into cavity 3 with the warm water through inlet tube 13, create comfortable temperature for cell reproduction.

The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included within the protection scope of the present invention.

Claims

1. A cell culture flask, characterized in that: comprises a bottle body (1) and a cork (12), wherein a cavity (3) is arranged in the bottle body (1), a drain pipe (15) is arranged at the bottom of the bottle body (1), a drain valve (16) is arranged on the drain pipe (15), a water inlet pipe (13) is arranged at the upper left part of the bottle body (1), a water inlet valve (14) is arranged on the water inlet pipe (13), a plurality of cell culture plates (2) are uniformly arranged at the bottom in the bottle body (1), the cell culture plates (2) are hollow, the bottoms of the cell culture plates (2) are communicated with the cavity (3), a heat preservation layer (4) is arranged at the outer side of the bottle body (1), two bottle openings (17) are arranged at the upper part of the bottle body (1), the bottle openings (17) are matched with the cork (12), a vent pipe (8) and a liquid suction pipe (5) are arranged on the cork (, be provided with on right side cork (12) and annotate liquid pipe (9) and permeability cell (8), it is located permeability cell (8) left side to annotate liquid pipe (9), be provided with hydrophobic ventilated membrane (7) on permeability cell (8), inside permeability cell (8) one end intercommunication atmosphere, other end intercommunication bottle (1), pipette (5) lower part straight-through bottle (1) bottom, pipette (5) upper portion is provided with small water pump (6), annotate inside liquid pipe (9) bottom intercommunication bottle (1), upper portion is provided with annotates liquid valve (10), annotates liquid pipe (9) top fixed connection bell (11).

2. A cell culture flask according to claim 1, wherein: the heat-insulating layer (4) is made of polyurethane foam.

3. A cell culture flask according to claim 1, wherein: the vent pipe (8), the pipette (5), the liquid injection pipe (9) and the cork (12) are detachably connected.