CN107668722A - Date polysaccharide, and preparation method thereof and the food containing date polysaccharide - Google Patents
Date polysaccharide, and preparation method thereof and the food containing date polysaccharide Download PDFInfo
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Abstract
The date polysaccharide that the present invention provides a kind of preparation method of date polysaccharide, is prepared by the preparation method, and the food containing date polysaccharide.The food of date polysaccharide is prepared by date polysaccharide and Co-Q10 etc..Granule, capsule, tablet, drink, oral liquid, instant food etc. can be prepared into.Date polysaccharide has anticancer, anti-AIDS, anti-oxidant, decompression, regulation blood fat, antiallergy, raising immunity of organisms, mitigation poisonous substance to physiologically actives such as liver damages.Date polysaccharide can be used as sports tonica or health products.Food containing date polysaccharide has antiviral, raising immunity and other effects.
Description
Technical field
The invention provides a kind of preparation method of date polysaccharide, the date polysaccharide being prepared by the preparation method, and
Food containing date polysaccharide, belongs to field of food.
Background technology
Jujube, FRUCTUS JUJUBAE, for being dried to for Rhamnaceae deciduous tree jujube Ziziphus jujuba Mill.
Ripe fruit, it is a kind of nutrient excellent product, is described as " kings of all kinds of fruits ".Autumn harvests, and dries, raw to use.Used time breaks or is enucleated.Property
It is sweet, warm, returns spleen, stomach, there is tonifying middle-Jiao and Qi, nourishing blood and tranquilization, the function of relaxing the property of medicine；Jujube is mainly containing betulinic acid, olive
The organic acids such as acid, crataegolic acid, date polysaccharide.Its decoction have enhancing immune, increase muscular strength, reduce cholesterol, protect liver, it is anti-oxidant,
Suppress the effect such as cancer cell multiplication and anti-mutation.Often feeding jujube can treat in poor health, neurasthenia, incoordination between the spleen and the stomach, digestion not
Good, epersalgia cough, anaemia are become thin, and nourishing the liver preventing cancer function is especially prominent, " eating three jujube within 1st, beauty bears one's age well ".Yulin is good
County（Also known as Jia Zhou）Oily jujube is the king in jujube.《Beijing Tongrentang will》Record：" with the big jujubes of Jia Zhou, doctor's all kinds of diseases and ailments of being used as medicine ".
Co-Q10 (CoenzymeQ10) also known as Ubidecarenone, are a kind of fat-soluble antioxidants, are that human life can not
One of important element lacked.The nutrition of energy human activin cell and cellular energy, have and improve body immunity, enhancing antioxygen
The functions such as change, anti-aging and enhancing human activity.
N-octacosanol chemical name is n- n-octacosanols, English name l-Octa-cosanol；n-
Octacosanol；Polieosanol；Trade name is Montanylalcohol, and Japan is referred to as Ocatacosanol, and nature is typically in the form of wax ester
In the leaf, stem, fruit or the epidermis that many plants be present, in such as apple, grape, sugarcane, wheat and rice food, contain two
Octadecanol.The generally acknowledged function factor with clear and definite health-care effect of domestic and international medical field is obtained in recent years.N-octacosanol has
There are enhancing endurance, energy and muscle power, improve muscular strength and reaction is keen, strengthen heart body, eliminate myalgia, reduce muscle
Friction, strengthens the repellence to altitude effect, changes the ratio of metabolism, reduces necessary oxygen demand, stimulates sex hormone, drop
Lower shrinkage phase blood pressure and other effects, it is a kind of preferably sports food hardening agent.
At present, the research of Polyose extraction is concentrated mainly on conventional hot water's extraction, occurs ultrasound, microwave novel auxiliary in recent years
Help the research of extractive technique.Research is found：Date polysaccharide have improve immunity of organisms, it is anti-oxidant, suppress cancer cell multiplication and
The physiologically actives such as anticomplement.Big jujube products in the market are mainly：Chinese date juice, jujube acid using jujube as main component
The products such as milk, jujube effervescent tablet.The polysaccharide of great activity value in jujube is extracted, the example being applied in food is less,
It is and less as the food research of active component using date polysaccharide.
The content of the invention
It is an object of the invention to provide a kind of extracting method of date polysaccharide, the date polysaccharide being prepared by this method,
And the function type health care food using date polysaccharide as main component.It has expanded the extraction and preparation technique of date polysaccharide, have developed
A kind of jujube food nutritious, that there is antiviral raising immunity function, so as to solve existing date polysaccharide application model
Enclose the problem of small, economic value is low.
The invention provides a kind of preparation method of date polysaccharide, it is characterised in that is prepared in accordance with the following steps：
80-100 DEG C of drying jujube, jujube crush, and dry powder adds water, solid-liquid ratio 1:6-12, Plus acidic albumen complex enzyme（Cellulose
Enzyme：Pectase：Acid protease=1:1:1）1.0-3.0%, adjust pH=4-5.5,40-50 DEG C of bath temperature, water bath time
100-150 minutes, water-bath are extracted 2 times, are warming up to 90 DEG C, keep 30min enzyme deactivations, are cooled down, and centrifugation, take upper strata extract solution, are merged
Extract solution, concentrated extracting solution, 60-100 DEG C of temperature are concentrated into half-three/mono- of former extract solution, and concentrate adds second
Alcohol, to determining alcohol 60-80%, 1-3 hours are stood, centrifugation, obtain date polysaccharide extract, are spray-dried, its EAT 120-
135 DEG C, 80-95 DEG C of leaving air temp ,-the 20ml of inlet amount 12/min, the 10-0.20 MPa of admission pressure 0..Wherein, it is acid
Albumen complex enzyme preferred content is 1.0%；PH is preferably 5.2；Preferably 50 DEG C of bath temperature；Water bath time preferably 120 minutes.
Precision measures extract solution 1ml, is diluted to 9ml, takes 50ul, the mL of phenol solution 1.8 for adding volume fraction to be 5%, shakes
It is even, enriching sulfuric acid 7mL, mix, stand 30min, it is 0.627 that its light absorption value is determined at maximum wavelength 494nm.Recovery rate is：
More than 58.10 %.
Present invention also offers a kind of active ingredient compositions of date polysaccharide extract, and it includes or by following component group
Into：
Date polysaccharide extract 30-60 parts by weight, Co-Q10 1-3 parts by weight.
Present invention also offers a kind of active ingredient compositions of extract containing date polysaccharide, it is consisted of the following composition：
Date polysaccharide extract 30-60 parts by weight, Co-Q10 1-3 parts by weight, n-octacosanol 0.3-1 parts by weight.
Above-mentioned composition can be further prepared into granule, capsule, tablet, drink, oral liquid, instant food, and its is equal
It can be prepared according to the conventional fabrication process method of this area.
Present invention also offers a kind of preparation method of date polysaccharide beverage：
（1）Weigh raw material date polysaccharide extract respectively, Co-Q10, n-octacosanol, Tween 80,
（2）The n-octacosanol of 1 parts by weight is added into 6 parts by weight Tween 80s and is prepared into n-octacosanol emulsion, by date polysaccharide
Extract presses 1：5 ratio, which is dissolved in pure water, is made date polysaccharide solution, then by n-octacosanol emulsion, Co-Q10 point
It is not added in date polysaccharide solution, mixes, filter, sterilizing, produces date polysaccharide beverage, wherein, date polysaccharide extract is
30-60 parts by weight, n-octacosanol emulsion are 3-7 parts by weight, Co-Q10 is 1-3 parts by weight.
Present invention also offers date polysaccharide, the active ingredient compositions containing date polysaccharide be used for it is antiviral, improve it is immune
The health-care effect of power, specific experiment content see below.
Advantages of the present invention.
（1）Date polysaccharide is soluble in water, and the organic solvent insoluble in high concentration, high temperature easily loses activity, peracid or meta-alkali
It will make polysaccharide that Partial digestion occur.The present invention is prepared height using the method for enzymolysis process and contained using jujube as raw material
The date polysaccharide of amount, important technical foundation is provided to deeply develop the polysaccharide of jujube, with huge consumption market and very
High economic value；
Date polysaccharide is jujube effect material base, have anticancer, anti-AIDS, it is anti-oxidant, decompression, regulation blood fat, antiallergy,
Improve immunity of organisms, mitigate poisonous substance to physiologically actives such as liver damages.Particularly, date polysaccharide also has resisting kinetic fatigue
Effect, locomitivity can be improved by increasing sugar deposit, energy source of the body in prolonged exercise is ensure that, prolong
The generation of slow sports fatigue, and be advantageous to slow down in motion process motion can be used as to the excessive use of protein, date polysaccharide
Tonic or health products.
（2）The product of the present invention is easy to carry, and conveniently takes, is the pure of the modern that rhythm of life is fast, operating pressure is big
Natural health invigorant.
（3）Preparation technology is simple, and production cost is low, is easy to batch production to produce in batches.
（4）Present invention firstly provides preparing active ingredient compositions by main component of date polysaccharide, by date polysaccharide and
With functional material n-octacosanol, Co-Q10 conjunctive use, compounded in specific proportions, there is excellent collaboration
Effect, can antiviral, raising immunity.
（5）Date polysaccharide beverage is effectively to combine date polysaccharide and beverage, and product gives full play to every kind of group in preparation
Point the characteristics of, product is larger played healthcare function, as n-octacosanol dissolubility is poor, then using emulsification form, increase by two
The dissolubility of octadecanol.
Embodiment
Embodiment 1：The preparation of date polysaccharide：
80 DEG C of drying jujubes, jujube crush, and dry powder adds water, solid-liquid ratio 1:6, Plus acidic albumen complex enzyme（Cellulase：Pectin
Enzyme：Acid protease=1:1:1）3.0%, adjust pH=5.2,50 DEG C of bath temperature, water bath time 100 minutes, water-bath extraction 2
It is secondary, 90 DEG C are warming up to, keeps 30min enzyme deactivations, is cooled down, centrifugation, takes upper strata extract solution, merges extract solution, concentrated extracting solution, temperature
60 DEG C, 1/3rd of former extract solution is concentrated into, concentrate adds ethanol, to determining alcohol 80%, stands 3 hours, centrifugation, obtains jujube
Polyoses extract, spray drying, 120 DEG C of its EAT, 80 DEG C, inlet amount 12ml/min of leaving air temp, admission pressure 0.
10MPa。
Embodiment 2：The preparation of date polysaccharide：
90 DEG C of drying jujubes, jujube crush, and dry powder adds water, solid-liquid ratio 1:10, Plus acidic albumen complex enzyme（Cellulase：Pectin
Enzyme：Acid protease=1:1:1）1.0%, adjust pH=4.5,40 DEG C of bath temperature, water bath time 120 minutes, water-bath extraction 2
It is secondary, 90 DEG C are warming up to, keeps 30min enzyme deactivations, is cooled down, centrifugation, takes upper strata extract solution, merges extract solution, concentrated extracting solution, temperature
80 DEG C, 1/3rd of former extract solution is concentrated into, concentrate adds ethanol, to determining alcohol 75%, stands 3 hours, centrifugation, obtains jujube
Polyoses extract, spray drying, 130 DEG C of its EAT, 80 DEG C, inlet amount 20ml/min of leaving air temp, admission pressure
0.20MPa。
Embodiment 3：A kind of active ingredient compositions containing date polysaccharide, said composition are prepared into functional health-care food,
It includes following component composition：The parts by weight of date polysaccharide extract 40 prepared by embodiment 2, the parts by weight of Co-Q10 3.
Embodiment 4：A kind of active ingredient compositions containing date polysaccharide, said composition are prepared into functional health-care food,
It is consisted of the following composition：The parts by weight of date polysaccharide extract 60 prepared by embodiment 2, the parts by weight of Co-Q10 3, octacosane
The parts by weight of alcohol 0.6.
Embodiment 5：The preparation of date polysaccharide beverage：
（1）Weigh raw material date polysaccharide extract respectively, Co-Q10, n-octacosanol, Tween 80,
（2）The n-octacosanol of 1 parts by weight is added into 6 parts by weight Tween 80s and is prepared into n-octacosanol emulsion, by date polysaccharide
Extract presses 1：5 ratio, which is dissolved in pure water, is made date polysaccharide solution, then by n-octacosanol emulsion, Co-Q10 point
It is not added in date polysaccharide solution, mixes, filter, sterilizing, produces date polysaccharide beverage, wherein, date polysaccharide extract is
30 parts by weight, n-octacosanol emulsion are 3 parts by weight, Co-Q10 is 3 parts by weight.
Embodiment 6：The preparation of date polysaccharide beverage：
（1）Weigh raw material date polysaccharide extract respectively, Co-Q10, n-octacosanol, Tween 80,
（2）The n-octacosanol of 1 parts by weight is added into 6 parts by weight Tween 80s and is prepared into n-octacosanol emulsion, by date polysaccharide
Extract presses 1：5 ratio, which is dissolved in pure water, is made date polysaccharide solution, then by n-octacosanol emulsion, Co-Q10 point
It is not added in date polysaccharide solution, mixes, filter, sterilizing, produces date polysaccharide beverage, wherein, date polysaccharide extract is
40 parts by weight, n-octacosanol emulsion are 6 parts by weight, Co-Q10 is 3 parts by weight.
Present invention also offers date polysaccharide, active ingredient compositions containing date polysaccharide be used for it is antiviral, improve immunity
Health-care effect.
Antivirus test.
50 Kunming mouses, male and female half and half, 20 ± 2g of body weight, are randomly divided into 5 groups, are respectively normal per big group 10
Control group (normal group), virus control group（Model group）, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment.2 groups of embodiment, reality
Applying 3 groups of example, the component of embodiment 4 also known as takes the product of 10g embodiments 2, the product of 10g embodiments 3, the product of 10g embodiments 4 to add
Distilled water is stirred and evenly mixed, every m L of 20 g body weight gavage 0. 4 to 200mL, 1 time a day, continuous 3 d；Control group is (normal
Group), virus control group（Model group）Isometric physiological saline is given, 1 time a day, continuous 3 d.It is 4th day, slight with ether
After anesthetized mice, normal group is with physiological saline collunarium, the remaining group of H1N1virus mouse lung adapted strain collunarium with 10LD50
Infection, every 25 μ L, 4h starts to continue gastric infusion, one time a day, the 2d after virus infection after collunarium modeling（6d）,
6d is administered altogether.7th day mouse is broken after neck execution, is taken right side lungs, is entered in the 1.5m L Eppendof pipes that autoclaving is crossed,
1m L Trizol are added, extraction -70 DEG C of Liquid nitrogen storages of viral RNA are standby.
Above-mentioned viral RNA is taken to carry out one-step method real-time fluorescent quantitative polyase chain reaction（RT-PCR）, reaction volume is
The μ L of 20 uL, reaction system 2uL containing sample rna, One Step SYBR CREEN 16. 4,10 pmol H1N1 primers（On
Swim 5'-CACCAATCCTCTCACCTCTCAC-3 ' and downstream 5'-CCCATTTCCACAAACCTCTACC-3'）The μ L of primer 0. 8,
10 pmol house-keeping gene CAPDH primers（5'CCTCAACCTCCCTCTCAACC-3 ' and 5'-TCCCTCCTCCAACATCCTC-
3’）The μ L of primer 0. 8.Reaction condition is:42 DEG C of 5 min, 95 DEG C of 10 s, 95 DEG C of 5 s, 60 DEG C of 34 s, totally 40 are followed
Ring.Reaction carries out melting curve analysis after terminating, to identify the specificity of PCR primer.Use Sequence Detection
System software analysis PCR processes respectively detect the Ct values of sample.
Virus load computational methods:The method that this experiment uses relative quantification, using CAPDH as internal reference, select one
Sample (Con) is used as Calibrator, and computational methods are as follows:
Conk △Ct=Con Ct - Con GAPDH Ct
Sample △ Ct=sample Ct-sample GAPDH Ct
Sample △ △ Ct=sample △ Ct-Conk △ Ct
2- sample △ △ CtIt is that multiple of each treatment group gene expression with respect to Calibrator changes, is referred to as relative virus load
Virus load inhibiting rate=（The embodiment group virus load average of model group virus load average one)/model group virus load
Average x 100%.
Influence of each example composition to H1N1virus carrying capacity
| Group | Normal group | Model group | 2 groups of embodiment | 3 groups of embodiment | 4 groups of embodiment |
| With respect to virus load | - | 5589±1327 | 2763±1057* | 2068±981* | 849±762* |
| Virus load inhibiting rate | - | - | 50.56 | 63.01 | 84.8 |
Note:Each group is compared with model group, * P<0.05.
Conclusion：4 groups of 2 groups of embodiment, 3 groups of embodiment, embodiment compositions can significantly reduce mouse Influenza A H1N1
Virus load, 2 groups of virus load inhibiting rates of embodiment are 50.56%, and 3 groups of virus load inhibiting rates of embodiment are 63.01%, are implemented
4 groups of virus load inhibiting rates of example are 84.8%.It can be seen that 4 groups of 2 groups of embodiment, 3 groups of embodiment, embodiment compositions have it is antiviral
Effect.
Improve immunity test.
50 Kunming mouses, male and female half and half, 20 ± 2g of body weight, are randomly divided into 5 groups, are respectively normal per big group 10
Control group (normal group), cyclophosphamide-a control group（Model group）, 2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment.Model group, reality
4 groups of 2 groups of example, 3 groups of embodiment, embodiment endoxan physiological saline for 75mg/kg being subcutaneously injected daily in the 1-3 days are applied, normally
The 1-3 days physiological saline that equivalent is subcutaneously injected daily of control group.2 groups of embodiment, 3 groups of embodiment, 4 groups of embodiment 1-7 days are every
Natural gift also known as take the product of 10g embodiments 2, the product of 10g embodiments 3, the product of 10g embodiments 4 to add distilled water to 200mL,
Stir and evenly mix, every m L of 20 g body weight gavage 0. 4,1 time a day；Normal group (normal group), cyclophosphamide-a control group（Mould
Type group）Isometric physiological saline is given, 1 time a day, continuous 7d.
Intraperitoneal injection in 8th day 5% chicken red blood cell normal saline suspension 0.5ml, 8h put to death mouse, sterilize belly, abdominal cavity
The mL of Han Shi liquid 2.5 is injected, mouse web portion is gently rubbed, takes peritoneal fluid 0.5ml to be placed on slide, 30 are incubated in 37 DEG C of incubators
Min, physiological saline wash away the cell floated, Wright's stain dyeing, rinse, dry, micro- 200 macrophages of Microscopic observation,
And count the number of macrophages of phagocytosis chicken red blood cell, by phagocytosis chicken red blood cell number, every group of mouse peritoneal is calculated as follows
Phagocytic rate and phagocytic index:
Macrophage/200 macrophage the * 100% of phagocytic rate=phagocytosis phagocytosis chicken red blood cell
Phagocytic index=by phagocytosis chicken red blood cell number/200 macrophage ÷ 2
Separately take the spleen of each group mouse and thymus gland to weigh, and calculate within 8th day：
Index and spleen index=spleen weight（mg）/ body weight（g）
Thymus index=thymic weight（mg）/ body weight（g）.
Influence of each example composition to immunologic function
| Group | Normal group | Model group | 2 groups of embodiment | 3 groups of embodiment | 4 groups of embodiment |
| Phagocytic rate | 44.2±3.4 | 25.8±1.7△ | 32.4±1.8△* | 33.1±2.4△* | 35.6±2.2△* |
| Phagocytic index | 0.69±0.13 | 0.28±0.1△ | 0.38±0.16△* | 0.40±0.21△* | 0.46±0.18△* |
| Index and spleen index | 5.3±0.6 | 2.1±0.3△ | 3.5±0.5△* | 3.4±0.6△* | 4.1±0.6△* |
| Thymus index | 3.6±0.5 | 1.1±0.3△ | 1.4±0.3△* | 1.5±0.4△* | 1.8±0.4△* |
Note:Each group is compared with normal group, △ P<0.05；Each group is compared with model group, * P<0.05
Model group Turnover of Mouse Peritoneal Macrophages phagocytic rate, phagocytic index, index and spleen index, thymus index more normally significantly reduce,
Illustrate modeling success.
Conclusion：2 groups of embodiment, 3 groups of embodiment, embodiment 4 groups of Turnover of Mouse Peritoneal Macrophages phagocytic rates, phagocytic index, spleens
Dirty index, thymus index significantly raise compared with model group.It can be seen that 2 groups of embodiment, 3 groups of embodiment, the set product of embodiment 4 have
Improve efficacy of immunity.Embodiment 4 groups of Turnover of Mouse Peritoneal Macrophages phagocytic rates, phagocytic index, index and spleen index, thymus indexs compared with
2 groups of embodiment significantly raises, and illustrates that 4 groups of embodiment has more excellent raising efficacy of immunity.
Claims (8)
1. a kind of date polysaccharide, it is characterised in that be prepared in accordance with the following steps：
80-100 DEG C of drying jujube, jujube crush, and dry powder adds water, solid-liquid ratio 1:6-12, Plus acidic albumen complex enzyme（Cellulose
Enzyme：Pectase：Acid protease=1:1:1）1.0-3.0%, adjust pH=4-5.5,40-50 DEG C of bath temperature, water bath time
100-150 minutes, water-bath are extracted 2 times, are warming up to 90 DEG C, keep 30min enzyme deactivations, are cooled down, and centrifugation, take upper strata extract solution, are merged
Extract solution, concentrated extracting solution, 60-100 DEG C of temperature are concentrated into half-three/mono- of former extract solution, and concentrate adds second
Alcohol, to determining alcohol 60-80%, 1-3 hours are stood, centrifugation, obtain date polysaccharide extract, are spray-dried, its EAT 120-
135 DEG C, 80-95 DEG C of leaving air temp ,-the 20ml of inlet amount 12/min, the 10-0.20 MPa of admission pressure 0..
2. date polysaccharide according to claim 1, it is characterised in that：Acidic protein complex enzyme preferred content is 1.0%；pH
Preferably 5.2；Preferably 50 DEG C of bath temperature；Water bath time preferably 120 minutes.
3. a kind of active ingredient compositions containing date polysaccharide, it includes or consisted of the following composition：
As the date polysaccharide 30-60 parts by weight described in claim 1, Co-Q10 1-3 parts by weight.
4. a kind of active ingredient compositions containing date polysaccharide, it is consisted of the following composition：
As the date polysaccharide 30-60 parts by weight described in claim 1, Co-Q10 1-3 parts by weight, n-octacosanol 0.3-1 weights
Measure part.
5. according to date polysaccharide or active ingredient compositions described in claim any one of 1-4, it is characterised in that：According to ability
Prepared by the conventional fabrication process method in domain, can be prepared into granule, capsule, tablet, drink, oral liquid, instant food.
6. a kind of date polysaccharide beverage, it is characterised in that be prepared in accordance with the following steps：：
（1）Weigh raw material date polysaccharide extract respectively, Co-Q10, n-octacosanol, Tween 80,
（2）The n-octacosanol of 1 parts by weight is added into 6 parts by weight Tween 80s and is prepared into n-octacosanol emulsion, by date polysaccharide
Extract presses 1：5 ratio, which is dissolved in pure water, is made date polysaccharide solution, then by n-octacosanol emulsion, Co-Q10 point
It is not added in date polysaccharide solution, mixes, filter, sterilizing, produces date polysaccharide beverage.
7. date polysaccharide beverage according to claim 6, it is characterised in that：Date polysaccharide extract be 30-60 parts by weight,
N-octacosanol emulsion is 3-7 parts by weight, Co-Q10 is 1-3 parts by weight.
8. the active ingredient compositions according to claim any one of 3-4 are in antiviral, raising immunity food is prepared
Application.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108850747A (en) * | 2018-07-26 | 2018-11-23 | 芜湖职业技术学院 | A kind of Poria cocos/jujube composite beverage and preparation method thereof |
| CN110101689A (en) * | 2019-05-24 | 2019-08-09 | 江西省科学院生物资源研究所 | N-octacosanol is preparing the application in preventing liver injury drug |
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| CN108850747A (en) * | 2018-07-26 | 2018-11-23 | 芜湖职业技术学院 | A kind of Poria cocos/jujube composite beverage and preparation method thereof |
| CN110101689A (en) * | 2019-05-24 | 2019-08-09 | 江西省科学院生物资源研究所 | N-octacosanol is preparing the application in preventing liver injury drug |
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