CN107647412A - A kind of preparation method of longan nuclear protein polypeptide chewable tablets - Google Patents
A kind of preparation method of longan nuclear protein polypeptide chewable tablets Download PDFInfo
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- CN107647412A CN107647412A CN201710908522.5A CN201710908522A CN107647412A CN 107647412 A CN107647412 A CN 107647412A CN 201710908522 A CN201710908522 A CN 201710908522A CN 107647412 A CN107647412 A CN 107647412A
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- longan
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- nuclear protein
- protein polypeptide
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Links
- 235000000235 Euphoria longan Nutrition 0.000 title claims abstract description 105
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 42
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 42
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 42
- 102000007999 Nuclear Proteins Human genes 0.000 title claims abstract description 38
- 108010089610 Nuclear Proteins Proteins 0.000 title claims abstract description 38
- 239000007910 chewable tablet Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 244000139609 Euphoria longan Species 0.000 title 1
- 240000001008 Dimocarpus longan Species 0.000 claims abstract description 104
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 33
- 239000000243 solution Substances 0.000 claims abstract description 30
- 239000000284 extract Substances 0.000 claims abstract description 26
- 239000000843 powder Substances 0.000 claims abstract description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000012460 protein solution Substances 0.000 claims abstract description 15
- 102000011931 Nucleoproteins Human genes 0.000 claims abstract description 14
- 108010061100 Nucleoproteins Proteins 0.000 claims abstract description 14
- 238000004519 manufacturing process Methods 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 13
- 238000001914 filtration Methods 0.000 claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000000975 co-precipitation Methods 0.000 claims abstract description 9
- 238000000227 grinding Methods 0.000 claims abstract description 8
- 238000001291 vacuum drying Methods 0.000 claims abstract description 6
- 108091005804 Peptidases Proteins 0.000 claims description 18
- 239000004365 Protease Substances 0.000 claims description 18
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 18
- 239000003513 alkali Substances 0.000 claims description 13
- 150000001875 compounds Chemical class 0.000 claims description 13
- 239000003337 fertilizer Substances 0.000 claims description 10
- HQKMJHAJHXVSDF-UHFFFAOYSA-L magnesium stearate Chemical compound [Mg+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O HQKMJHAJHXVSDF-UHFFFAOYSA-L 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 239000002245 particle Substances 0.000 claims description 10
- 241001474374 Blennius Species 0.000 claims description 8
- 235000002722 Dioscorea batatas Nutrition 0.000 claims description 8
- 235000006536 Dioscorea esculenta Nutrition 0.000 claims description 8
- 240000001811 Dioscorea oppositifolia Species 0.000 claims description 8
- 235000003416 Dioscorea oppositifolia Nutrition 0.000 claims description 8
- 244000241838 Lycium barbarum Species 0.000 claims description 8
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 8
- 244000185386 Thladiantha grosvenorii Species 0.000 claims description 8
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims description 8
- 210000000582 semen Anatomy 0.000 claims description 8
- 239000002893 slag Substances 0.000 claims description 8
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 claims description 5
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 5
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 5
- 229920002774 Maltodextrin Polymers 0.000 claims description 5
- 239000005913 Maltodextrin Substances 0.000 claims description 5
- 229930195725 Mannitol Natural products 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
- 238000004061 bleaching Methods 0.000 claims description 5
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- HEBKCHPVOIAQTA-NGQZWQHPSA-N d-xylitol Chemical compound OC[C@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-NGQZWQHPSA-N 0.000 claims description 5
- 235000019359 magnesium stearate Nutrition 0.000 claims description 5
- 229940035034 maltodextrin Drugs 0.000 claims description 5
- 239000000594 mannitol Substances 0.000 claims description 5
- 235000010355 mannitol Nutrition 0.000 claims description 5
- 229940069338 potassium sorbate Drugs 0.000 claims description 5
- 235000010241 potassium sorbate Nutrition 0.000 claims description 5
- 239000004302 potassium sorbate Substances 0.000 claims description 5
- 239000011122 softwood Substances 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000000080 wetting agent Substances 0.000 claims description 5
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 4
- 238000001035 drying Methods 0.000 claims description 4
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 4
- 239000000811 xylitol Substances 0.000 claims description 4
- 235000010447 xylitol Nutrition 0.000 claims description 4
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 4
- 229960002675 xylitol Drugs 0.000 claims description 4
- 239000013049 sediment Substances 0.000 claims 1
- 238000005516 engineering process Methods 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 238000011084 recovery Methods 0.000 abstract description 3
- 230000009849 deactivation Effects 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 description 17
- 230000007062 hydrolysis Effects 0.000 description 13
- 238000006460 hydrolysis reaction Methods 0.000 description 13
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- 102000004169 proteins and genes Human genes 0.000 description 8
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- 108010082495 Dietary Plant Proteins Proteins 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 235000019441 ethanol Nutrition 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 230000036541 health Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
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- 210000004369 blood Anatomy 0.000 description 3
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- 230000003301 hydrolyzing effect Effects 0.000 description 3
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- 229940045997 vitamin a Drugs 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 206010013774 Dry eye Diseases 0.000 description 1
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 1
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000014171 carbonated beverage Nutrition 0.000 description 1
- 230000008131 children development Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- NKLPQNGYXWVELD-UHFFFAOYSA-M coomassie brilliant blue Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=C1 NKLPQNGYXWVELD-UHFFFAOYSA-M 0.000 description 1
- 238000011161 development Methods 0.000 description 1
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- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 238000012869 ethanol precipitation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 239000008236 heating water Substances 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000009777 vacuum freeze-drying Methods 0.000 description 1
- 235000015192 vegetable juice Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/03—Products from fruits or vegetables; Preparation or treatment thereof consisting of whole pieces or fragments without mashing the original pieces
- A23L19/07—Fruit waste products, e.g. from citrus peel or seeds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/10—Products from fruits or vegetables; Preparation or treatment thereof of tuberous or like starch containing root crops
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
- A23L5/34—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using microwaves
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention relates to a kind of preparation method of longan nuclear protein polypeptide chewable tablets, belong to food technology field.The preparation method of the longan nuclear protein polypeptide chewable tablets, comprises the following steps：S1. crush：Longan seed is dried, ultramicro grinding, longan seed Ultramicro-powder is made；S2. extract：According to mass volume ratio it is 1 by longan seed Ultramicro-powder obtained by step S1 and water:8 12 dissolvings, adjust pH to 9 11, using ultrasonic microwave combined extracting, filtering, obtain extract solution, extract solution is precipitated using isoelectric point ethanol co precipitation method, vacuum drying, obtains longan nucleoprotein；S3. activated carbon decolorizing；S4. digest：The longan seed protein solution regulation pH value that step S3 is obtained is 7.2 7.6, enzymolysis, enzyme deactivation, filters, concentration, dries and longan nuclear protein polypeptide is made；S5. dispensing tabletting.The method of the invention recovery rate is high, and technique is simple, easy to operate, and cost is cheap, is adapted to industrialized production.
Description
【Technical field】
The present invention relates to food technology field, and in particular to a kind of preparation method of longan nuclear protein polypeptide chewable tablets.
【Background technology】
With the development of people's living standards continue to improve and modern nutriology, people recognize to take in excessive richness further
Food containing animal protein may bring the health problems such as high fat of blood, high cholesterol.By contrast, vegetable protein has
The advantages that low cholesterol and high protein, it is relatively beneficial to health.Vegetable protein beverage is nutritious, can provide needed by human body
Proteins,vitamins,and minerals etc., also tend to that there is certain health-care effect due to functional components specific to raw material.
Analysis display, the coming five years, China will adjust beverage industry structure, while continuing to improve production, give priority to fruit
The products such as vegetable juice beverage, vegetable protein beverage and tea beverage, the ratio of the carbonated beverages such as cola is reduced, develop simultaneously standard function
The production of property beverage.
Longan nucleoprotein is a kind of higher vegetable protein of nutritive value, and it contains 8 kinds of essential amino acids of needed by human body,
Easily digested and assimilated for human body, to safeguarding that health and early children development play an important roll.And longan nucleoprotein is made through digesting
Longan seed polypeptide can directly be absorbed without degraded by enteron aisle, infiltration rate and absorptivity ratio protein and amino acid are all high, and also
With health cares such as the growth metabolisms of profitable strain such as reducing blood lipid, norcholesterol, anti-oxidant, raising immunity, promotion Bifidobacterium
Effect.
Therefore, prepare a kind of longan nuclear protein polypeptide chewable tablets using longan seed and will be provided with good market prospects.
【The content of the invention】
The goal of the invention of the present invention is：For above-mentioned problem, there is provided a kind of longan nuclear protein polypeptide chewable tablets
Preparation method, methods described recovery rate is high, and technique is simple, easy to operate, and cost is cheap, is adapted to industrialized production.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of preparation method of longan nuclear protein polypeptide chewable tablets, comprises the following steps：
S1. crush：Longan seed is dried, ultramicro grinding, longan seed Ultramicro-powder is made；
S2. extract：According to mass volume ratio it is 1 by longan seed Ultramicro-powder obtained by step S1 and water:8-12 dissolves, and adjusts pH
To 9-11, using ultrasonic-microwave combined extracting, filtering, extract solution is obtained, extract solution uses isoelectric point-ethanol co precipitation
Method precipitates, and vacuum drying, obtains longan nucleoprotein；
S3. activated carbon decolorizing：The longan nucleoprotein that step S2 is obtained is added into water, regulation pH value to 4.0 is made into quality volume
Fraction is 8-12% solution, adds activated carbon, and the addition of activated carbon needs 1g activated carbons to calculate by every 100ml solution, filtering,
Obtain longan seed protein solution；
S4. digest：It is 7.2-7.6 by the longan seed protein solution regulation pH value that step S3 is obtained, enzymolysis, enzymolysis finishes
Afterwards, enzyme deactivation, filter, concentration, dry and longan nuclear protein polypeptide is made；
S5. dispensing tabletting：Take sorosis slag 50-60 parts by weight, purple Chinese yam 5-15 parts by weight, longan seed starch 5-15 weight
Part, step S4 longan nuclear protein polypeptide 0.5-0.8 parts by weight, seaweed 5-15 parts by weight, matrimony vine 5-10 parts by weight, Momordica grosvenori 2-
5 parts by weight, Semen Cuscutae 2-5 parts by weight, crush, cross 200-300 mesh sieves, mixing, obtain mixing Ultramicro-powder, then add maltodextrin
5-10 parts by weight, xylo-oligosaccharide 3-6 parts by weight, xylitol 5-10 parts by weight, mannitol 10-15 parts by weight, FOS 2-5 weights
Part, potassium sorbate 0.5-1 parts by weight and wetting agent 150-200 parts by weight are measured, softwood processed, 20 mesh sieves is crossed and particle is made, particle dries
Magnesium stearate 2-3 parts by weight are added after dry to mix, and tabletting, produce the longan nuclear protein polypeptide chewable tablets.
Further, in step S1, the ultramicro grinding is that the average grain diameter of longan seed Ultramicro-powder after crushing is 10~20 μ
m。
Further, in step S2, the ultrasonic-microwave combined extracting is to be in 60-70 DEG C of temperature, ultrasonic power
6-8min is extracted under 150-200W, microwave power 300w.
Further, in step S3, the condition of the activated carbon decolorizing is：80 DEG C of temperature, bleaching time 40min.
Further, in step S4, the enzymolysis is is 55-60 DEG C in temperature, under conditions of ultrasonic wave 70W, by 0.8g/
100ml-1g/100ml adds alkali protease, is hydrolyzed by 0.5g/100ml addition calcium chloride into the longan seed protein solution
After 2h-3h, then by 0.4g/100ml-0.6g/100ml addition compound fertilizer production hydrolysis 2h-3h.
Further, in step S4, the weight proportion of the alkali protease and the compound fertilizer production is 2:1.
Present invention additionally comprises the longan nuclear protein polypeptide chewable tablets that the above method is prepared.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
(1) present invention uses ultrasonic-microwave combined extracting, can not only retain longan nucleoprotein to greatest extent, and
And the recovery rate of longan nucleoprotein can be improved, while shorten extraction time.
(2) extract solution of the present invention is using isoelectric point-ethanol co precipitation method precipitation, and rate of deposition is higher, the time required to precipitation
It is shorter.
(3) present invention is under ultrasonic wave and zymoexciter synergy, using two kinds of protease (alkali protease and compound
Flavor protease) double enzymolysis method, protein hydrolysis degree and polypeptide yield are high.
(4) sorosis slag, purple Chinese yam and Semen Cuscutae nourish eyes in the raw material that chewable tablets of the present invention uses, and alleviate eyes and dry
Ache；Seaweed can maintain twenty-twenty vision rich in vitamin A, prevent eyes dry and astringent with it is tired；Matrimony vine is rich in carrotene, vitamin
A, B1, B2, C, calcium, iron etc., it is the required nutrition of healthy eye；Momordica grosvenori clearing heat and cooling blood, clear liver and improve vision；Longan nuclear protein polypeptide
Eye microcirculation can be improved, alleviate visual fatigue, Six-element natural plant composition acts synergistically with longan nuclear protein polypeptide, changed significantly
It has been apt to eyesight, has alleviated eye strain, safeguards glasses health.
【Embodiment】
Embodiment 1
A kind of preparation method of longan nuclear protein polypeptide chewable tablets, comprises the following steps：
S1. crush：Longan seed is dried, ultramicro grinding, the longan seed Ultramicro-powder that average grain diameter is 10~20 μm is made；
S2. extract：According to mass volume ratio it is 1 by longan seed Ultramicro-powder obtained by step S1 and water:10 dissolving, regulation pH to
10, using ultrasonic-microwave combined extracting, extracted at 65 DEG C, ultrasonic power 150-200W, microwave power 300w of temperature
6min, filtering obtain extract solution, and extract solution vacuum drying, obtains longan seed using isoelectric point-ethanol co precipitation method precipitation
Albumen；
S3. activated carbon decolorizing：The longan nucleoprotein that step S2 is obtained is added into water, regulation pH value to 4.0 is made into quality volume
Fraction is 10% solution, adds activated carbon, the addition of activated carbon needs 1g activated carbons to calculate by every 100ml solution, in temperature
Under conditions of 80 DEG C, bleaching time 40min, filtering, longan seed protein solution is obtained；
S4. digest：It is 7.2-7.6 by the longan seed protein solution regulation pH value that step S3 is obtained, is 55-60 in temperature
DEG C, under conditions of ultrasonic wave 70W, alkali protease is added by 0.8g/100ml, calcium chloride is added to described by 0.5g/100ml
After hydrolyzing 2h in longan seed protein solution, then by 0.4g/100ml addition compound fertilizer production hydrolysis 2h, after enzymolysis, go out
Enzyme, filter, longan nuclear protein polypeptide is made in concentration, freeze-drying；
S5. dispensing tabletting：Take the parts by weight of sorosis slag 50, the parts by weight of purple Chinese yam 5, longan seed starch 5 parts by weight, step S4
The parts by weight of longan nuclear protein polypeptide 0.5, the parts by weight of seaweed 5, the parts by weight of matrimony vine 5, the parts by weight of Momordica grosvenori 2, the parts by weight of Semen Cuscutae 2,
Crush, cross 200 mesh sieves, mixing, obtain mixing Ultramicro-powder, then add the parts by weight of maltodextrin 5, the parts by weight of xylo-oligosaccharide 3, xylose
The parts by weight of alcohol 5, the parts by weight of mannitol 10, the parts by weight of FOS 2, the parts by weight of potassium sorbate 0.5 and the parts by weight of wetting agent 150,
Softwood processed, cross 20 mesh sieves and particle is made, adding the parts by weight of magnesium stearate 2 after particle drying mixes, and tabletting, produces the longan seed
Polypeptide chewable tablets.
Embodiment 2
A kind of preparation method of longan nuclear protein polypeptide chewable tablets, comprises the following steps：
S1. crush：Longan seed is dried, ultramicro grinding, the longan seed Ultramicro-powder that average grain diameter is 10~20 μm is made；
S2. extract：According to mass volume ratio it is 1 by longan seed Ultramicro-powder obtained by step S1 and water:8 dissolving, regulation pH to
9, using ultrasonic-microwave combined extracting, extracted under temperature 60 C, ultrasonic power 150-200W, microwave power 300w
8min, filtering obtain extract solution, and extract solution vacuum drying, obtains longan seed using isoelectric point-ethanol co precipitation method precipitation
Albumen；
S3. activated carbon decolorizing：The longan nucleoprotein that step S2 is obtained is added into water, regulation pH value to 4.0 is made into quality volume
Fraction is 8% solution, adds activated carbon, and the addition of activated carbon needs 1g activated carbons to calculate, is in temperature by every 100ml solution
Under conditions of 80 DEG C, bleaching time 40min, filtering, longan seed protein solution is obtained；
S4. digest：It is 7.2-7.6 by the longan seed protein solution regulation pH value that step S3 is obtained, is 55-60 in temperature
DEG C, under conditions of ultrasonic wave 70W, alkali protease is added by 1g/100ml, calcium chloride is added to the dragon by 0.5g/100ml
After hydrolyzing 3h in eye nucleoprotein solution, then by 0.4g/100ml addition compound fertilizer production hydrolysis 2h, after enzymolysis, go out
Enzyme, filter, longan nuclear protein polypeptide is made in concentration, freeze-drying；
S5. dispensing tabletting：Take the parts by weight of sorosis slag 60, the parts by weight of purple Chinese yam 15, the parts by weight of longan seed starch 15, step S4
The parts by weight of longan nuclear protein polypeptide 0.8, the parts by weight of seaweed 15, the parts by weight of matrimony vine 10, the parts by weight of Momordica grosvenori 5, the weight of Semen Cuscutae 5
Part, crush, cross 300 mesh sieves, mixing, obtain mixing Ultramicro-powder, then add maltodextrin 10 parts by weight, the parts by weight of xylo-oligosaccharide 6,
The parts by weight of xylitol 10, the parts by weight of mannitol 15, the parts by weight of FOS 5, the parts by weight of potassium sorbate 1 and the weight of wetting agent 200
Part, softwood processed, cross 20 mesh sieves and particle is made, adding the parts by weight of magnesium stearate 3 after particle drying mixes, and tabletting, produces the dragon
Eye nuclear protein polypeptide chewable tablets.
Embodiment 3
A kind of preparation method of longan nuclear protein polypeptide chewable tablets, comprises the following steps：
S1. crush：Longan seed is dried, ultramicro grinding, the longan seed Ultramicro-powder that average grain diameter is 10~20 μm is made；
S2. extract：According to mass volume ratio it is 1 by longan seed Ultramicro-powder obtained by step S1 and water:12 dissolving, regulation pH to
11, using ultrasonic-microwave combined extracting, extracted under temperature 70 C, ultrasonic power 150-200W, microwave power 300w
7min, filtering obtain extract solution, and extract solution vacuum drying, obtains longan seed using isoelectric point-ethanol co precipitation method precipitation
Albumen；
S3. activated carbon decolorizing：The longan nucleoprotein that step S2 is obtained is added into water, regulation pH value to 4.0 is made into quality volume
Fraction is 12% solution, adds activated carbon, the addition of activated carbon needs 1g activated carbons to calculate by every 100ml solution, in temperature
Under conditions of 80 DEG C, bleaching time 40min, filtering, longan seed protein solution is obtained；
S4. digest：It is 7.2-7.6 by the longan seed protein solution regulation pH value that step S3 is obtained, is 55-60 in temperature
DEG C, under conditions of ultrasonic wave 70W, alkali protease is added by 0.8g/100ml, calcium chloride is added to described by 0.5g/100ml
After hydrolyzing 2h in longan seed protein solution, then by 0.6g/100ml addition compound fertilizer production hydrolysis 3h, after enzymolysis, go out
Enzyme, filter, longan nuclear protein polypeptide is made in concentration, freeze-drying；
S5. dispensing tabletting：Take the parts by weight of sorosis slag 55, the parts by weight of purple Chinese yam 10, the parts by weight of longan seed starch 10, step S4
The parts by weight of longan nuclear protein polypeptide 0.6, the parts by weight of seaweed 10, the parts by weight of matrimony vine 7, the parts by weight of Momordica grosvenori 3, the weight of Semen Cuscutae 3
Part, crush, cross 200 mesh sieves, mixing, obtain mixing Ultramicro-powder, then add maltodextrin 8 parts by weight, the parts by weight of xylo-oligosaccharide 5,
The parts by weight of xylitol 7, the parts by weight of mannitol 12, the parts by weight of FOS 3, the parts by weight of potassium sorbate 0.7 and the weight of wetting agent 180
Part, softwood processed, cross 20 mesh sieves and particle is made, adding the parts by weight of magnesium stearate 2.5 after particle drying mixes, and tabletting, produces described
Longan nuclear protein polypeptide chewable tablets.
Comparative example 1
This comparative example longan nuclear protein polypeptide chewable tablets, with embodiment 1, the difference with embodiment 1 is its preparation method：
Its raw material components used is not added with sorosis slag, purple Chinese yam, seaweed, Semen Cuscutae, matrimony vine, Momordica grosvenori Six-element natural plant composition,
Other component proportions are the same as embodiment 1.
Comparative example 2
This comparative example longan nuclear protein polypeptide chewable tablets, with embodiment 1, the difference with embodiment 1 is its preparation method：
Its raw material components used is not added with the longan nuclear protein polypeptide of the present invention, and other component proportions are the same as embodiment 1.
Experimental example 1：The comparison of longan nucleoprotein ultrasonic-microwave combined extracting and other method
Ultrasonic-microwave combined extracting and microwave radiation exaraction, ultrasound assisted extraction, the extraction of conventional heating water bath are carried out
Compare, as a result such as table 1.
The comparison of the Different Extraction Method of table 1
As can be seen from the above table, ultrasonic-microwave combined extracting of the invention and other extracting method ratios, there is extraction effect
The advantages that rate is high, extraction time is short and DNA purity is high.
The quality of the quality of protein/longan seed Ultramicro-powder total protein in longan seed protein extracting ratio (%)=extract solution ×
100%.
By extract solution protein precipitation, after freeze-drying, the quality of albumen in extract is determined with Coomassie Brilliant Blue, is calculated
Longan seed purity of protein, quality × 100% of quality/extract of albumen in purity of protein (%)=extract.
Experimental example 2：The selection of longan seed albumen precipitation method
Extract solution obtained by ultrasonic-microwave combined extracting is respectively taken into 5ml, isoelectric point precipitation, tetraploid is respectively adopted
After the product absolute ethyl alcohol precipitation method and isoelectric point ethanol co precipitation method precipitation, vacuum freeze drying, longan seed albumen precipitation is calculated
Rate, it is compared, is shown in Table 2.
The selection of the longan seed albumen precipitation method of table 2
| Project | The time required to precipitation (min) | Rate of deposition (%) |
| Isoelectric point precipitation | 5-8 | 80.3 |
| Ethanol precipitation | 120-220 | 93.5 |
| Isoelectric point-ethanol co precipitation method | 5-8 | 98.6 |
As can be seen from the above table, the intermediate processing of present invention selection selection isoelectric point ethanol synergy, the electricity such as combine
The advantages of point settling velocity is fast, and alcohol precipitation is complete, rate of deposition can be made to reach 98.6%, be only 5 minutes the time required to precipitation.
Albumen quality × 100% in albumen quality/extract solution of longan seed albumen precipitation rate (%)=precipitation.
Experimental example 3：The screening of protease
On the basis of alkali protease and the single enzyme hydrolysis of compound fertilizer production, in order to reach higher degree of hydrolysis,
Using ultrasonic wave and the lower alkali protease of zymoexciter synergy and compound fertilizer production double enzymolysis method, first basic protein
Enzyme digests, rear compound fertilizer production enzymolysis, as a result such as table 3.
3 single enzyme of table and double enzyme enzymolysis process effects compare
As can be seen from the above table, alkali protease enzymolysis gained longan nuclear protein polypeptide degree of hydrolysis is minimum, composite flavor egg
The longan nuclear protein polypeptide degree of hydrolysis of white enzyme enzymolysis gained is 26.7%, higher than alkali protease.But through alkali protease and again
The degree of hydrolysis for closing sequential hydrolysis after flavor protease compounds significantly improves, and through ultrasonic wave and zymoexciter lower pair of enzyme of synergy
The degree of hydrolysis highest of method of double crossing enzymolysis.Therefore this experiment determine longan seed proteolysis final technique be defined as ultrasonic wave with
Under zymoexciter synergy, first with the double enzyme method of double crossing hydrolyzed after hydrolysis by novo with compound fertilizer production.
The measure of degree of hydrolysis (DH) of the present invention uses pH-Star methods.
Compliance test result
To verify the effect of product of the present invention, 100 volunteers of random selection, the observation of taking carried out 60 days by a definite date tries
Test, the product truly has curative effect to eyesight improving, is first reported as follows：
100 volunteers are equally divided into 5 groups, respectively using embodiment 1-3 and comparative example 1-2 chewable tablets, continuously made
With (application method after 60 days：Orally, everyone 2 times a day, each one), count curative effect, wherein, eye fatigue disappear, depending on thing
Clearly, and with 5 meters of logarithmic visual acuity charts of standard test, eyesight lifts 1 row or 1 row above person, is effective；Eye fatigue, regarding thing
Definition has improvement, and is tested with 5 meters of logarithmic visual acuity charts of standard, and eyesight is lifted less than 1 passerby, is effective；Eye fatigue, regarding thing
Definition is without any improvement, is invalid.Result of the test is shown in Table 1.
The using effect of table 1 is evaluated
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | |
| It is effective | 11 | 13 | 10 | 6 | 7 |
| Effectively | 7 | 6 | 8 | 9 | 7 |
| It is invalid | 2 | 1 | 2 | 5 | 6 |
| Total effective rate | 90% | 95% | 90% | 75% | 70% |
As seen from the above table, chewable tablets of the invention, i.e., including sorosis slag, purple Chinese yam, seaweed, Semen Cuscutae, matrimony vine, Momordica grosvenori
The effect of Six-element natural plant composition and longan nuclear protein polypeptide will be substantially than only adding Six-element natural plant composition or longan seed egg
The chewable tablets effect of white polypeptide will get well, therefore the synergy of Six-element natural plant composition and longan nuclear protein polypeptide is obvious, energy
Enough it is effectively improved eyesight.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of preparation method of longan nuclear protein polypeptide chewable tablets, it is characterised in that comprise the following steps：
S1. crush：Longan seed is dried, ultramicro grinding, longan seed Ultramicro-powder is made；
S2. extract：According to mass volume ratio it is 1 by longan seed Ultramicro-powder obtained by step S1 and water:8-12 dissolves, and adjusts pH to 9-
11, using ultrasonic-microwave combined extracting, filtering, extract solution is obtained, extract solution is sunk using isoelectric point-ethanol co precipitation method
Form sediment, vacuum drying, obtain longan nucleoprotein；
S3. activated carbon decolorizing：The longan nucleoprotein that step S2 is obtained is added into water, regulation pH value to 4.0 is made into quality volume fraction
For 8-12% solution, activated carbon is added, the addition of activated carbon needs 1g activated carbons to calculate, filtering, obtained by every 100ml solution
Longan seed protein solution；
S4. digest：It is 7.2-7.6 by the longan seed protein solution regulation pH value that step S3 is obtained, digests, after enzymolysis, go out
Enzyme, filter, concentration, dry and longan nuclear protein polypeptide is made；
S5. dispensing tabletting：Take sorosis slag 50-60 parts by weight, purple Chinese yam 5-15 parts by weight, longan seed starch 5-15 parts by weight, step
Rapid S4 longan nuclear protein polypeptide 0.5-0.8 parts by weight, seaweed 5-15 parts by weight, matrimony vine 5-10 parts by weight, Momordica grosvenori 2-5 weight
Part, Semen Cuscutae 2-5 parts by weight, crush, cross 200-300 mesh sieves, mixing, obtain mixing Ultramicro-powder, then add maltodextrin 5-10
Parts by weight, xylo-oligosaccharide 3-6 parts by weight, xylitol 5-10 parts by weight, mannitol 10-15 parts by weight, FOS 2-5 weight
Part, potassium sorbate 0.5-1 parts by weight and wetting agent 150-200 parts by weight, softwood processed, cross 20 mesh sieves and particle, particle drying is made
Magnesium stearate 2-3 parts by weight are added afterwards to mix, and tabletting, produce the longan nuclear protein polypeptide chewable tablets.
2. according to the method for claim 1, it is characterised in that in step S1, the ultramicro grinding is longan seed after crushing
The average grain diameter of Ultramicro-powder is 10~20 μm.
3. according to the method for claim 1, it is characterised in that in step S2, the ultrasonic-microwave combined extracting be
60-70 DEG C of temperature, 6-8min is extracted under ultrasonic power 150-200W, microwave power 300w.
4. according to the method for claim 1, it is characterised in that in step S3, the condition of the activated carbon decolorizing is：Temperature
80 DEG C, bleaching time 40min.
5. according to the method for claim 1, it is characterised in that it is described to digest to be 55-60 DEG C in temperature in step S4,
Under conditions of ultrasonic wave 70W, alkali protease is added by 0.8g/100ml-1g/100ml, calcium chloride is added by 0.5g/100ml
After 2h-3h being hydrolyzed into the longan seed protein solution, then by 0.4g/100ml-0.6g/100ml addition compound fertilizer productions
Hydrolyze 2h-3h.
6. according to the method for claim 5, it is characterised in that in step S4, the alkali protease and the compound wind
The weight proportion of taste protease is 2:1.
7. the longan nuclear protein polypeptide chewable tablets that any one of claim 1-6 methods described is prepared.
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