CN107576683A - A kind of rapid identification method of Moringa cold resistance - Google Patents
A kind of rapid identification method of Moringa cold resistance Download PDFInfo
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Abstract
The present invention discloses a kind of rapid identification method of Moringa cold resistance, including the part for choosing some Moringa plant branch tip 10cm 20cm to be identified cuts off blade and side shoot and cleaned up, blot the moisture on examination material surface as examination material；Examination material is cut into the segment of 0.8 1.2cm length, is sufficiently mixed and at least 5 groups of samples are made；Taking one of which sample, each group sample in addition is low temperature stress sample as check sample；Each low temperature stress sample is positioned over the low temperature stress processing for carrying out 8h 16h in refrigeration plant by the low temperature stress temperature of different gradients after being packaged in polybag, then thaws at room temperature；Boiling preceding electric conductivity value and boiling rear electric conductivity value for the outer sepage of measure each group sample, calculates the cell damage rate of each low temperature stress sample, finally utilizes the half lethal low temperature LT of cell damage rate score, stress temperature and Logistic equations calculating Moringa50, LT50The cold resistance of the lower explanation kind of value is stronger.The present invention can rapid evaluation Moringa kind to be identified cold-resistant ability.
Description
Technical field
The present invention relates to the technical field of the cold-resistant ability characterization and evaluation of plant, especially a kind of quick mirror of Moringa cold resistance
Determine method.
Background technology
Moringa (Moringa spp.) is also known as drumstick tree, strange tree, category Moringaceae (Moringaceae) Moringa (Moringa
Adans.), it is a kind of torrid zone, subtropical zone multi-functional plant, rich in a variety of nutrition such as vegetable protein, vitamin, folic acid, calcium, iron
Composition, there is unique economic value and health-care efficacy.
Moringa is suitable for the torrid zone and South Subtropical Area of China plantation, and cold resistance is to restrict it to middle subtropical zone and more backlands area pushes away
Wide biggest obstacle.The research both at home and abroad to Moringa is concentrated mainly on medical value and feed conversion etc. at present, and to peppery
The research of wooden cold resistance yet there are no play-by-play, and the characterization and evaluation of Moringa cold resistance is not sought unity of standard also.Therefore carry out peppery
Wooden variety source cold resistance Rapid identification research, the popularization and cold-resistant breeding to Moringa are significant.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of rapid identification method of Moringa cold resistance, it can rapid evaluation
The cold-resistant ability of Moringa kind to be identified, be advantageous to the seed selection and popularization of the cold-resistant kind of Moringa.
To reach above-mentioned purpose, the technical scheme is that：A kind of rapid identification method of Moringa cold resistance, including with
Lower step：
Step 1: the part for choosing some Moringa plant branch tip 10cm-20cm to be identified cuts off blade as examination material
And side shoot；Then after being cleaned up with water, blot or dry the moisture on examination material surface；
Step 2: the examination material after blotting or drying be cut into 0.8-1.2cm length segment, be divided into after being sufficiently mixed quantity or
The roughly the same n group samples of quality, n >=5；
Step 3: one of which sample is taken not do low temperature stress as check sample；Other each group sample is coerced for low temperature
Compel sample, each low temperature stress sample is positioned over the low temperature stress that 8h-16h is carried out in refrigeration plant, each group after being packaged in polybag
The stress temperature of low temperature stress sample is followed successively by t2、t3……tn, tn-1-tn=t0, wherein t2For 3 DEG C -5 DEG C, t0For 3 DEG C -5 DEG C,
The low temperature stress time of each group low temperature stress sample is identical；
Step 4: after the low temperature stress time arrives, each group low temperature stress sample is taken out from refrigeration plant, then 20 DEG C-
25 DEG C of defrosting 1h-2h at room temperature；
Step 5: 2g-5g is taken respectively in each group low temperature stress sample and the check sample of non-Stress treatment after thawing
Sample, the sample quality taken out in each group sample is identical, is then respectively put into corresponding test tube, sample is added into each test tube
The deionized water of 8-12 times of quality, the deionized water added in each test tube it is identical in quality；Then each test tube is put into closed appearance
In device, pumping process 10min-20min, the pumping process time phase of each test tube then are carried out to closed container using vavuum pump
Together, followed by each test tube is taken out, preceding electric conductivity value is boiled with conductivity gauge measure each test tube China and foreign countries sepage；It is put into followed by each test tube
10min-20min is boiled in 100 DEG C of boiling water baths, the time that each test tube boils in 100 DEG C of boiling water baths is identical, and it is cold to then take out each test tube
But to 20 DEG C -25 DEG C of room temperature, recycle conductivity gauge measure each test tube China and foreign countries sepage boils rear electric conductivity value；Wherein set L11To be right
Preceding electric conductivity value, L are boiled in this outer sepage in the same old way21Rear electric conductivity value is boiled for sepage outside check sample, outside other each low temperature stress samples
The boil preceding electric conductivity value and the rear electric conductivity value that boils of outer sepage of sepage are respectively L12、L22, L13、L23……L1n、L2n；
Step 6: utilize the electric conductivity value of said determination and calculation formula y (%)=[1- (1-L of cell damage rate1n/
L2n)/(1-L11/L21)] × 100% calculate each group sample through low temperature stress cell damage rate, obtain through each of low temperature stress
The cell damage rate of group sample is y2,y3……yn；
Step 7: utilize the above-mentioned cell damage rate y calculated2,y3……ynWith Logistic equations " y=k/ (1+
a·e-bt) " calculate Moringa half lethal low temperature LT50, in Logistic equations, y is the above-mentioned cell damage calculated
Rate, that is, correspond to y2,y3……yn, k is the saturation value of cell damage rate, and cell damage rate eliminates background interference k values in this method
It is stress temperature for 100%, t, that is, corresponds to t2、t3……tn, a, b are parameter；Logistic equations are first converted into ln (k-
Y)/y=lna-bt, it is the cell damage rate after conversion to make y '=ln (k-y)/y=lna-bt, y ', corresponding using temperature t is coerced
Cell damage rate y ' values after conversion obtain linear regression equation and parameter a, b according to the method for linear regression equation, then right
Linear regression equation carries out second dervative and becomes the knee value lna/b, as semilethal low temperature LT for getting equation curve in return50=lna/b；
According to the half lethal low temperature LT calculated50, it can be determined that the cold resistance of the Moringa kind, low temperature semilethal temperature
Spend LT50The cold resistance of the lower explanation kind is stronger.
Further preferably, in the step 1, some Moringa plant branch tip 10cm-15cm to be identified part is chosen
As examination material, use running water, deionized water rinsing clean successively after cutting off blade and side shoot, then blot selection examination with blotting paper
The moisture on material surface.Identification speed can so be improved.
It is preferred that the refrigeration plant is refrigerator, in step 3, each group of low temperature stress sample is placed in a refrigerator.Choosing
Equipment investment can be reduced with refrigerator.
It is preferred that described room temperature is 25 DEG C.
Further preferably, in the step 2, n=7, that is, it is divided into quantity or roughly the same 7 groups of quality after being sufficiently mixed
Sample.Select 7 groups of sample number to play preferable balance to the accuracy of identification speed and identification, both reduced input, and can improves
Efficiency, it is ensured that the accuracy of identification.
Still further preferably, in step 3, each low temperature stress sample is positioned in refrigeration plant after being packaged in polybag
Row 8h low temperature stress.
Still further preferably, in step 3, t2For 4 DEG C, t0For 4 DEG C, the stress temperature of each group low temperature stress sample is followed successively by
4 DEG C, 0 DEG C, -4 DEG C, -8 DEG C, -12 DEG C and -16 DEG C.
Further, the refrigeration plant from 20 DEG C of -25 DEG C of room temperatures be down to it is described stress temperature rate of temperature fall for 4 DEG C/
h。
Further preferably, in step 5, each group low temperature stress sample and the check sample of non-Stress treatment after thawing
The middle sample for taking 2g respectively, then it is respectively put into corresponding test tube, the deionized water of 10 times of sample quality is added into each test tube,
20ml deionized water is added, the capacity of each test tube is 50ml, and each test tube is put into sealing container and carried out at 10min pumping
Reason.
Further preferably, in step 3, check sample once it is determined that, i.e., according to step 5 method measure check sample outside
Preceding electric conductivity value L is boiled in sepage11Rear electric conductivity value L is boiled with outer sepage21.The speed of identification can so be accelerated.
Beneficial effects of the present invention are：
1st, the present invention is using Moringa branch tip 10cm-20cm part as examination material, because this is partially in semi-lignified
State, its plant cell is more sensitive to cold environment, and by low temperature stress, it is more objective and accurate to determine its cold-resistant ability, so
Make afterwards by rational sample and the determination of sample size, electric conductivity value and outer sepage before the boiling of outer sepage by determining sample
Boil rear electric conductivity value, can comparatively fast calculate its cell damage rate, then utilize Logistic equations " y=k/ (1+ae-bt)”、
Cell damage rate and corresponding stress temperature can quickly calculate the half lethal low temperature LT of Moringa50.It is low according to what is calculated
Warm semilethal low temperature LT50, it can be determined that the cold resistance of the Moringa kind, half lethal low temperature LT50The lower explanation kind
Cold resistance is stronger.This authentication method is capable of the cold-resistant ability of rapid evaluation Moringa kind to be identified, is advantageous to the cold-resistant product of Moringa
The seed selection and popularization of kind.
2nd, in electric conductivity value before determining the boiling of outer sepage, each test tube is put into closed container, then utilizes vavuum pump pair
Closed container carries out pumping process, to extract space between cells air out, moisture is easily infiltrated into space between cells, intracellular solute is easy
In oozing out, effectively shorten detection time, be also beneficial to the accuracy for improving detection.
3rd, this method is easy to operate, and the cycle is short, and accuracy is high, and measure can be repeated several times, can provide objective, quantized data,
It is adapted to the quick screening of lot of materials.
4th, this method equipment is simple, and in addition to refrigeration plant, other equipment is relatively cheap, and appraisal cost is low, beneficial to popularization.
Embodiment
The present invention is described in further detail with reference to specific embodiment.
The lifes in 4 years that present embodiment chooses ' oil Drumstick Tree powder ', ' XIABANLAMU ', ' PKM1 ' this 3 kind Moringas are good for
Health plant carries out identification operation three times, confession examination plant is colonized in Fujian Tropical crop science and ground as identification object
Study carefully institute's Moringa Germplasm Resources.
Embodiment one, 4 years raw healthy plants of two ' oil Drumstick Tree powder ' are randomly selected as identification object.A kind of Moringa is resistance to
The rapid identification method of cold property, comprises the following steps：
Step 1, some Moringa plant branch tips to be identified are randomly selected in the plant of two ' oil Drumstick Tree powder '
10cm-15cm part is as examination material, and the branch of selection must be healthy branch, and the part particularly chosen is not by disease pest case shadow
Ring, then cut off blade and side shoot；It is clean with running water, deionized water rinsing successively, blot selection examination material surface with blotting paper
Moisture.
Step 2, the examination material after blotting is cut into the segment of 0.8-1.2cm length, quantity is divided into after being sufficiently mixed or quality is big
Cause 7 groups of samples of identical.
Step 3: one of which sample is taken not do low temperature stress as check sample；6 groups of other samples are coerced for low temperature
Compel sample, each low temperature stress sample is positioned over the low temperature stress that 8h is carried out in corresponding refrigerator, each group low temperature after being packaged in polybag
The stress temperature of stress sample is followed successively by 4 DEG C, 0 DEG C, -4 DEG C, -8 DEG C, -12 DEG C and -16 DEG C, the low temperature of each group low temperature stress sample
Stress time is identical；And refrigerator can be set and be down to the rate of temperature fall of the stress temperature as 4 DEG C/h from 25 DEG C of room temperatures.
Step 4: after the low temperature stress time arrives, each group low temperature stress sample is taken out from each refrigerator, then 25 DEG C of room
The lower defrosting 1h-2h of temperature；
Step 5: 2g sample is taken in each group low temperature stress sample and the check sample of non-Stress treatment after thawing respectively
This, is then respectively put into corresponding test tube, and the deionized water of 10 times of sample quality is added into each test tube, that is, adds going for 20ml
Ionized water, the capacity from each test tube are 50ml；Then each test tube is put into closed container, then using vavuum pump to close
Close container and carry out pumping process 10min, the pumping process time of each test tube is identical, followed by each test tube is taken out, uses conductivity gauge
Preceding electric conductivity value is boiled in each test tube China and foreign countries sepage of (DDS-12 types) measure；Boiled followed by each test tube is put into 100 DEG C of boiling water baths
10min, the time that each test tube boils in 100 DEG C of boiling water baths is identical, then takes out the room temperature that each test tube is cooled to 25 DEG C, recycles
Rear electric conductivity value is boiled in conductivity gauge (DDS-12 types) measure each test tube China and foreign countries sepage；Wherein set L11Boiled for sepage outside check sample
Preceding electric conductivity value, L21Rear electric conductivity value is boiled for sepage outside check sample, and the outer sepage of other each low temperature stress samples boils preceding electric conductivity value
The rear electric conductivity value that boils with outer sepage is respectively L12、L22, L13、L23……L1n、L2n；
Above-mentioned check sample electric conductivity value measure, check sample once it is determined that, you can according to step 5 method measure pair
Preceding electric conductivity value L is boiled in this outer sepage in the same old way11Rear electric conductivity value L is boiled with outer sepage21.The speed of identification can so be accelerated.
Step 6: utilize the electric conductivity value of said determination and calculation formula y (%)=[1- (1-L of cell damage rate1n/
L2n)/(1-L11/L21)] × 100% calculate each group sample through low temperature stress cell damage rate, obtain through each of low temperature stress
The cell damage rate of group sample is y2,y3……yn；
Step 7: utilize the above-mentioned cell damage rate y calculated2,y3……ynWith Logistic equations " y=k/ (1+
a·e-bt) " calculate Moringa half lethal low temperature LT50, in Logistic equations, y is the above-mentioned cell damage calculated
Rate, that is, correspond to y2,y3……yn, k is the saturation value of cell damage rate, and cell damage rate eliminates background interference k values in this method
It is stress temperature for 100%, t, that is, corresponds to t2、t3……tn, a, b are parameter；Logistic equations are first converted into ln (k-
Y)/y=lna-bt, it is the cell damage rate after conversion to make y '=ln (k-y)/y=lna-bt, y ', corresponding using temperature t is coerced
Cell damage rate y ' values after conversion obtain linear regression equation and parameter a, b according to the method for linear regression equation, then right
Linear regression equation carries out second dervative and becomes the knee value lna/b, as semilethal low temperature LT for getting equation curve in return50=lna/b；
According to the half lethal low temperature LT calculated50, it can be determined that the cold resistance of the Moringa kind, low temperature semilethal temperature
Spend LT50The cold resistance of the lower explanation kind is stronger.
On Logistic equations " y=k/ (1+ae-bt) " specific conversion process it is as follows：
Y=k/ (1+ae-bt)
=>y(1+a·e-bt)=k
=>y+y·a·e-bt=k
=>y·a·e-bt=k-y
=>a·e-bt=(k-y)/y
Both sides take natural logrithm to obtain
=>ln(a·e-bt)=ln (k-y)/y
=>lna+lne-bt=ln (k-y)/y
=>Lna-bt=ln (k-y)/y
=>Ln (k-y)/y=lna-bt
Y '=ln (k-y)/y is made, then：(in formula, y ' is transformed cells injury rate to y '=lna-bt, and t is the stress temperature of sample
Degree), y ' and t forms linear relationship.
Above-mentioned identification, which can also choose other low temperature stress samples and be packaged in after polybag to be positioned in corresponding refrigerator, to be carried out
16h low temperature stress, half lethal low temperature LT is then calculated again50。
Embodiment two, 4 years raw healthy plants of two ' XIABANLAMU ' are randomly selected as identification object.A kind of Moringa is resistance to
The rapid identification method of cold property, its identify the step of it is identical with embodiment one.
Embodiment three, 4 years raw healthy plants of two ' PKM1 ' are randomly selected as identification object.A kind of Moringa cold resistance
Rapid identification method, its identify the step of it is identical with embodiment one.
The Logistic equations and half lethal low temperature LT of three embodiments50It is as shown in the table：
Table：The Logistic equations and half lethal low temperature (LT of 3 different Moringa kinds50)
As can be seen from the above table, the cold-resistant ability of XIABANLAMU is most strong, next to that PKM1 Moringas, are thirdly that heavy wool is peppery
Wood.Illustrate its cold-resistant ability by being followed successively by ' XIABANLAMU ' to weak by force>‘PKM1’>' oil Drumstick Tree powder '.
In addition, as seen from table, the degree of fitting R of three kind Moringa Logistic equations2It is when low temperature stress handles 8h
0.9195~0.9270, it is 0.8159~0.8600 when low temperature stress handles 8h, each fit equation has preferably fitting knot
Fruit.The degree of fitting of equation is higher than low temperature stress processing 16h degree of fitting during certain low temperature stress processing 8h, the accuracy of identification
More preferably.
When low temperature stress processing time is 8h, the LT of ' XIABANLAMU '50For -7.62 DEG C, the LT of ' PKM1 '50For -7.25
DEG C, the LT of ' oil Drumstick Tree powder '50For -6.57 DEG C；When low temperature stress processing time is 16h, the LT of ' XIABANLAMU '50For -5.67
DEG C, the LT of ' PKM1 '50For -5.21 DEG C, the LT of ' oil Drumstick Tree powder '50For -4.15 DEG C.Although same kind under the different disposal time
The half lethal low temperature that calculates is different, but between the kind drawn half lethal low temperature height sequence it is consistent,
The repeatability of result of the test is preferably.
This authentication method can also handle the accuracy for demonstrating Moringa examination material half lethal low temperature, low temperature stress from recovery
Handle 8h Moringa branch sample it is recovered after, 4 DEG C, 0 DEG C and -4 DEG C processing electrolyte osmotic rates slightly reduce, illustrate carefully
After birth still has recovery capability, and the electrolyte osmotic rate of -8 DEG C, -12 DEG C and -16 DEG C processing slightly raises, and illustrates that cell membrane is damaged
It has been unable to full recovery.Wherein electrolyte osmotic rate (%)=(L1/L2) × 100%, L1 boils preceding electric conductivity value, L2 for outer sepage
Rear electric conductivity value is boiled for outer sepage.The half lethal low temperature LT that this result is calculated with upper table50Match, further test
Demonstrate,prove the feasibility of above-mentioned authentication method.
Embodiment above is not the concrete restriction to the present invention merely to the more preferable explanation present invention, this area
Technical staff is made the protection domain that equivalent change both falls within this case by claim.
Claims (10)
1. a kind of rapid identification method of Moringa cold resistance, comprises the following steps：
Step 1: the part for choosing some Moringa plant branch tip 10cm-20cm to be identified cuts off blade and side as examination material
Branch；Then after being cleaned up with water, blot or dry the moisture on examination material surface；
Step 2: the examination material after blotting or drying is cut into the segment of 0.8-1.2cm length, quantity or quality are divided into after being sufficiently mixed
Roughly the same n group samples, n >=5；
Step 3: one of which sample is taken not do low temperature stress as check sample；Other each group sample is low temperature stress sample
This, each low temperature stress sample is positioned over the low temperature stress that 8h-16h is carried out in refrigeration plant, each group low temperature after being packaged in polybag
The stress temperature of stress sample is followed successively by t2、t3……tn, tn-1-tn=t0, wherein t2For 3 DEG C -5 DEG C, t0For 3 DEG C -5 DEG C, each group
The low temperature stress time of low temperature stress sample is identical；
Step 4: after the low temperature stress time arrives, each group low temperature stress sample is taken out from refrigeration plant, then at 20 DEG C -25 DEG C
Defrosting 1h-2h at room temperature；
Step 5: 2g-5g sample is taken in each group low temperature stress sample and the check sample of non-Stress treatment after thawing respectively
This, the sample quality taken out in each group sample is identical, is then respectively put into corresponding test tube, sample quality is added into each test tube
8-12 times of deionized water, the deionized water added in each test tube it is identical in quality；Then each test tube is put into closed container,
Then pumping process 10min-20min is carried out to closed container using vavuum pump, the pumping process time of each test tube is identical, then connects
And take out each test tube, preceding electric conductivity value is boiled with conductivity gauge measure each test tube China and foreign countries sepage；100 DEG C of boilings are put into followed by each test tube
10min-20min is boiled in water-bath, the time that each test tube boils in 100 DEG C of boiling water baths is identical, then takes out each test tube and is cooled to 20
DEG C -25 DEG C of room temperature, recycle conductivity gauge measure each test tube China and foreign countries sepage boils rear electric conductivity value；Wherein set L11For check sample
Preceding electric conductivity value, L are boiled in outer sepage21Rear electric conductivity value is boiled for sepage outside check sample, the outer sepage of other each low temperature stress samples
The rear electric conductivity value that boils for boiling preceding electric conductivity value and outer sepage is respectively L12、L22, L13、L23……L1n、L2n；
Step 6: utilize the electric conductivity value of said determination and calculation formula y (%)=[1- (1-L of cell damage rate1n/L2n)/(1-
L11/L21)] × 100% calculate each group sample through low temperature stress cell damage rate, obtain each group sample through low temperature stress
Cell damage rate be y2,y3……yn；
Step 7: utilize the above-mentioned cell damage rate y calculated2,y3……ynWith Logistic equations " y=k/ (1+ae-bt) " calculate Moringa half lethal low temperature LT50, in Logistic equations, y is the above-mentioned cell damage rate calculated, i.e.,
Correspond to y2,y3……yn, k is the saturation value of cell damage rate, and cell damage rate eliminates background interference k values and is in this method
100%, t are stress temperature, that is, correspond to t2、t3……tn, a, b are parameter；First Logistic equations be converted into ln (k-y)/
Y=lna-bt, it is the cell damage rate after conversion to make y '=ln (k-y)/y=lna-bt, y ', and using coercing, temperature t is corresponding to be turned
Cell damage rate y ' values after change obtain linear regression equation and parameter a, b according to the method for linear regression equation, then to straight
Line regression equation carries out second dervative and becomes the knee value lna/b, as semilethal low temperature LT for getting equation curve in return50=lna/b；
According to the half lethal low temperature LT calculated50, it can be determined that the cold resistance of the Moringa kind, half lethal low temperature
LT50The cold resistance of the lower explanation kind is stronger.
A kind of 2. rapid identification method of Moringa cold resistance according to claim 1, it is characterised in that：The step 1
In, some Moringa plant branch tip 10cm-15cm to be identified part is chosen as examination material, is cut off after blade and side shoot successively
It is clean with running water, deionized water rinsing, the moisture for choosing examination material surface is then blotted with blotting paper.
A kind of 3. rapid identification method of Moringa cold resistance according to claim 1, it is characterised in that：The refrigeration plant
For refrigerator, in step 3, each group of low temperature stress sample is placed in a refrigerator.
A kind of 4. rapid identification method of Moringa cold resistance according to claim 1, it is characterised in that：Described room temperature is
25℃。
A kind of 5. rapid identification method of Moringa cold resistance according to claim 1, it is characterised in that：The step 2
In, n=7, that is, it is divided into quantity or the roughly the same 7 groups of samples of quality after being sufficiently mixed.
A kind of 6. rapid identification method of Moringa cold resistance according to claim 5, it is characterised in that：In step 3, respectively
Low temperature stress sample is positioned over progress 8h low temperature stress in refrigeration plant after being packaged in polybag.
A kind of 7. rapid identification method of Moringa cold resistance according to claim 5, it is characterised in that：In step 3, t2For
4 DEG C, t0For 4 DEG C, the stress temperature of each group low temperature stress sample is followed successively by 4 DEG C, 0 DEG C, -4 DEG C, -8 DEG C, -12 DEG C and -16 DEG C.
A kind of 8. rapid identification method of Moringa cold resistance according to claim 7, it is characterised in that：The refrigeration plant
The rate of temperature fall that the stress temperature is down to from 20 DEG C of -25 DEG C of room temperatures is 4 DEG C/h.
A kind of 9. rapid identification method of Moringa cold resistance according to claim 1, it is characterised in that：In step 5,
Each group low temperature stress sample after defrosting and the sample for taking 2g in the check sample of non-Stress treatment respectively, are then respectively put into pair
Answer in test tube, the deionized water of 10 times of sample quality is added into each test tube, that is, add 20ml deionized water, the appearance of each test tube
Measure and be put into the pumping process that sealing container carries out 10min for 50ml, each test tube.
A kind of 10. rapid identification method of Moringa cold resistance according to any one of claim 1 to 9, it is characterised in that：Step
In rapid three, check sample once it is determined that, i.e., according to electric conductivity value L before the boiling of sepage outside the method measure check sample of step 511With
Rear electric conductivity value L is boiled in outer sepage21。
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Cited By (3)
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| CN108645893A (en) * | 2018-04-26 | 2018-10-12 | 北京林业大学 | A method of evaluation plum blossom winter resistance |
| CN109521128A (en) * | 2019-01-15 | 2019-03-26 | 中国农业科学院特产研究所 | A kind of method of the overwintering winter resistance index of correlation analysis of V. amurensis |
| CN114009326A (en) * | 2021-11-05 | 2022-02-08 | 河北省农林科学院滨海农业研究所 | Method for rapidly identifying salt tolerance of in-vitro water culture of plant |
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