CN107494354A - A kind of holothurian culture method - Google Patents
A kind of holothurian culture method Download PDFInfo
- Publication number
- CN107494354A CN107494354A CN201710824839.0A CN201710824839A CN107494354A CN 107494354 A CN107494354 A CN 107494354A CN 201710824839 A CN201710824839 A CN 201710824839A CN 107494354 A CN107494354 A CN 107494354A
- Authority
- CN
- China
- Prior art keywords
- parts
- powder
- culture method
- sea cucumber
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000251511 Holothuroidea Species 0.000 title claims abstract description 87
- 238000012136 culture method Methods 0.000 title claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 41
- 239000000284 extract Substances 0.000 claims abstract description 38
- 239000011669 selenium Substances 0.000 claims abstract description 28
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 25
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 24
- 244000276331 Citrus maxima Species 0.000 claims abstract description 19
- 235000001759 Citrus maxima Nutrition 0.000 claims abstract description 19
- 239000007788 liquid Substances 0.000 claims abstract description 15
- 238000012258 culturing Methods 0.000 claims abstract description 14
- 235000012054 meals Nutrition 0.000 claims abstract description 14
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims abstract description 12
- 239000011734 sodium Substances 0.000 claims abstract description 12
- 229910052708 sodium Inorganic materials 0.000 claims abstract description 12
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 11
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 11
- 235000008434 ginseng Nutrition 0.000 claims abstract description 11
- 239000004575 stone Substances 0.000 claims abstract description 7
- RJFAYQIBOAGBLC-UHFFFAOYSA-N 2-amino-4-methylselanyl-butanoic acid Chemical compound C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 241000512259 Ascophyllum nodosum Species 0.000 claims abstract description 6
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims abstract description 6
- 244000017020 Ipomoea batatas Species 0.000 claims abstract description 6
- 235000002678 Ipomoea batatas Nutrition 0.000 claims abstract description 6
- 241000276489 Merlangius merlangus Species 0.000 claims abstract description 6
- 235000019482 Palm oil Nutrition 0.000 claims abstract description 6
- 241000237509 Patinopecten sp. Species 0.000 claims abstract description 6
- 241000593522 Sargassum thunbergii Species 0.000 claims abstract description 6
- 241000196251 Ulva arasakii Species 0.000 claims abstract description 6
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims abstract description 6
- 239000002540 palm oil Substances 0.000 claims abstract description 6
- 235000020637 scallop Nutrition 0.000 claims abstract description 6
- 239000000052 vinegar Substances 0.000 claims abstract description 6
- 235000021419 vinegar Nutrition 0.000 claims abstract description 6
- 241000195474 Sargassum Species 0.000 claims abstract description 5
- 230000009278 visceral effect Effects 0.000 claims abstract description 4
- 241000238366 Cephalopoda Species 0.000 claims abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 51
- 238000002360 preparation method Methods 0.000 claims description 38
- 239000006041 probiotic Substances 0.000 claims description 27
- 235000018291 probiotics Nutrition 0.000 claims description 27
- 238000001035 drying Methods 0.000 claims description 19
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 15
- 230000033228 biological regulation Effects 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 13
- 239000002955 immunomodulating agent Substances 0.000 claims description 12
- 230000002584 immunomodulator Effects 0.000 claims description 12
- 229940121354 immunomodulator Drugs 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 11
- 239000000706 filtrate Substances 0.000 claims description 10
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 10
- 240000004371 Panax ginseng Species 0.000 claims description 9
- 230000008859 change Effects 0.000 claims description 8
- 241000765216 Hymeniacidon perlevis Species 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims description 7
- 230000001900 immune effect Effects 0.000 claims description 7
- 239000000243 solution Substances 0.000 claims description 7
- 241000317936 Siganus guttatus Species 0.000 claims description 6
- 239000003814 drug Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 5
- 239000004382 Amylase Substances 0.000 claims description 5
- 102000013142 Amylases Human genes 0.000 claims description 5
- 108010065511 Amylases Proteins 0.000 claims description 5
- 241000205585 Aquilegia canadensis Species 0.000 claims description 5
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims description 5
- 235000017491 Bambusa tulda Nutrition 0.000 claims description 5
- 241001647745 Banksia Species 0.000 claims description 5
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims description 5
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims description 5
- 235000009685 Crataegus X maligna Nutrition 0.000 claims description 5
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims description 5
- 235000009486 Crataegus bullatus Nutrition 0.000 claims description 5
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims description 5
- 235000009682 Crataegus limnophila Nutrition 0.000 claims description 5
- 240000000171 Crataegus monogyna Species 0.000 claims description 5
- 235000004423 Crataegus monogyna Nutrition 0.000 claims description 5
- 235000002313 Crataegus paludosa Nutrition 0.000 claims description 5
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims description 5
- 235000014375 Curcuma Nutrition 0.000 claims description 5
- 244000164480 Curcuma aromatica Species 0.000 claims description 5
- 240000009138 Curcuma zedoaria Species 0.000 claims description 5
- 235000003405 Curcuma zedoaria Nutrition 0.000 claims description 5
- 244000111489 Gardenia augusta Species 0.000 claims description 5
- 235000018958 Gardenia augusta Nutrition 0.000 claims description 5
- 239000009636 Huang Qi Substances 0.000 claims description 5
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 claims description 5
- 241000108664 Nitrobacteria Species 0.000 claims description 5
- 244000082204 Phyllostachys viridis Species 0.000 claims description 5
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims description 5
- 241000490453 Rorippa Species 0.000 claims description 5
- 241000220317 Rosa Species 0.000 claims description 5
- 235000019418 amylase Nutrition 0.000 claims description 5
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 5
- 239000011425 bamboo Substances 0.000 claims description 5
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 5
- 239000001812 curcuma zedoaria berg. rosc. Substances 0.000 claims description 5
- VFLDPWHFBUODDF-FCXRPNKRSA-N curcumin Chemical compound C1=C(O)C(OC)=CC(\C=C\C(=O)CC(=O)\C=C\C=2C=C(OC)C(O)=CC=2)=C1 VFLDPWHFBUODDF-FCXRPNKRSA-N 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 5
- 239000004310 lactic acid Substances 0.000 claims description 5
- 235000014655 lactic acid Nutrition 0.000 claims description 5
- 238000002386 leaching Methods 0.000 claims description 5
- 229910052760 oxygen Inorganic materials 0.000 claims description 5
- 239000001301 oxygen Substances 0.000 claims description 5
- 239000002245 particle Substances 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- 239000003223 protective agent Substances 0.000 claims description 5
- 239000002994 raw material Substances 0.000 claims description 5
- -1 regulation pH Substances 0.000 claims description 5
- 235000019509 white turmeric Nutrition 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 4
- 244000274847 Betula papyrifera Species 0.000 claims description 3
- 235000009113 Betula papyrifera Nutrition 0.000 claims description 3
- 235000009109 Betula pendula Nutrition 0.000 claims description 3
- 235000010928 Betula populifolia Nutrition 0.000 claims description 3
- 235000002992 Betula pubescens Nutrition 0.000 claims description 3
- 230000008901 benefit Effects 0.000 claims description 3
- 238000005904 alkaline hydrolysis reaction Methods 0.000 claims description 2
- 239000011536 extraction buffer Substances 0.000 claims description 2
- 239000011780 sodium chloride Substances 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 241000193830 Bacillus <bacterium> Species 0.000 claims 1
- 244000025254 Cannabis sativa Species 0.000 claims 1
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 239000011814 protection agent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 5
- 230000036541 health Effects 0.000 abstract description 3
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 230000009977 dual effect Effects 0.000 abstract description 2
- 230000001988 toxicity Effects 0.000 abstract description 2
- 231100000419 toxicity Toxicity 0.000 abstract description 2
- 241000208340 Araliaceae Species 0.000 abstract 2
- 229940091258 selenium supplement Drugs 0.000 description 20
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 14
- 201000010099 disease Diseases 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 6
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 241000195493 Cryptophyta Species 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 230000001717 pathogenic effect Effects 0.000 description 5
- 244000063299 Bacillus subtilis Species 0.000 description 4
- 235000014469 Bacillus subtilis Nutrition 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- 241000243142 Porifera Species 0.000 description 4
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 4
- 239000002131 composite material Substances 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 244000052769 pathogen Species 0.000 description 4
- 241000238371 Sepiidae Species 0.000 description 3
- 239000003513 alkali Substances 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 235000008216 herbs Nutrition 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 230000004083 survival effect Effects 0.000 description 3
- 241001290610 Abildgaardia Species 0.000 description 2
- 241000728028 Kentrocapros rosapinto Species 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000012000 cholesterol Nutrition 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 2
- 239000003651 drinking water Substances 0.000 description 2
- 235000020188 drinking water Nutrition 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 210000000987 immune system Anatomy 0.000 description 2
- 230000007233 immunological mechanism Effects 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000008944 intestinal immunity Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000021368 organ growth Effects 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 229960001471 sodium selenite Drugs 0.000 description 2
- 239000011781 sodium selenite Substances 0.000 description 2
- 235000015921 sodium selenite Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 210000001835 viscera Anatomy 0.000 description 2
- 241000251730 Chondrichthyes Species 0.000 description 1
- 244000000626 Daucus carota Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000258955 Echinodermata Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003035 anti-peroxidant effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 235000005770 birds nest Nutrition 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 102000023852 carbohydrate binding proteins Human genes 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000036737 immune function Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000004347 intestinal mucosa Anatomy 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108010038196 saccharide-binding proteins Proteins 0.000 description 1
- 150000003342 selenium Chemical class 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- 235000005765 wild carrot Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/30—Culture of aquatic animals of sponges, sea urchins or sea cucumbers
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/30—Oligoelements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/742—Spore-forming bacteria, e.g. Bacillus coagulans, Bacillus subtilis, clostridium or Lactobacillus sporogenes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/66—Microorganisms or materials therefrom
- A61K35/74—Bacteria
- A61K35/741—Probiotics
- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/23—Apiaceae or Umbelliferae (Carrot family), e.g. dill, chervil, coriander or cumin
- A61K36/232—Angelica
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
- A61K36/285—Aucklandia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/35—Caprifoliaceae (Honeysuckle family)
- A61K36/355—Lonicera (honeysuckle)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/74—Rubiaceae (Madder family)
- A61K36/744—Gardenia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/752—Citrus, e.g. lime, orange or lemon
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/75—Rutaceae (Rue family)
- A61K36/756—Phellodendron, e.g. corktree
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
- A61K36/8998—Hordeum (barley)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
- A61K36/9066—Curcuma, e.g. common turmeric, East Indian arrowroot or mango ginger
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Polymers & Plastics (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Alternative & Traditional Medicine (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Marine Sciences & Fisheries (AREA)
- Environmental Sciences (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biochemistry (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of holothurian culture method, including culturing pool management, young ginseng period feeds sargassum thunbergii and grinds liquid, fed into the ginseng phase and add selenium feed, there is provided sea cucumber to grow required nutriment, without any toxicity and side effects, not only Se content is high for obtained sea cucumber, nutritive value is high simultaneously, meets modern to health and the dual requirementses of nutrition.Described plus selenium feed is made up of sodium humate, Hu pummelo peel extract, degumming Kelp Powder, sargassum powder, scallop digested tankage, shrimp shell meal, medical stone powder, squid visceral meal, oyster shell whiting, sweet potato leaves powder, sea lettuce, palm oil, light-coloured vinegar, selenium methionine.
Description
Technical field
The present invention relates to aquaculture of aquatic animal technical field, and in particular to a kind of holothurian culture method.
Background technology
Sea cucumber, belong to Holothuroidea, be the ocean echinoderm to 8000 meters by the sea that lives, gone through away from modern existing more than 600,000,000 years
History, sea cucumber is using seabed algae and planktonic organism as food.Sea cucumber whole body covers with corn, is widely distributed in each ocean in the world.South China Sea
Littoral species is more, and there are about more than 20 kind sea cucumbers can be edible.
The effect of sea cucumber, is very more with acting on, and not only mouthfeel is very well and nutritive value is higher for it, and sea cucumber contains protein, fat
Fat, carbohydrate, calcium, phosphorus, iron, and vitamin b1, vitamin b2, niacin etc..Sea cucumber is extremely low containing cholesterol, for one kind
Typical high protein, low fat, low cholesterol food.Plus its fine and tender taste, it is easy to digest, so, it is extremely suitable for old age
People, children and people of weak constitution eat.
The same ginseng of sea cucumber, bird's nest, shark's fin are equally celebrated for their achievements, are one of eight big treasures of the world.Sea cucumber is not only the food of preciousness, and
Rare medicinal material.According to《A Supplement to the Compendium of Materia Medica》Described in：Sea cucumber, sweet-salty, kidney tonifying, benefiting quintessence, urine is taken the photograph, impotence is treated in establishing-Yang, its
Warm-natured benefit, sufficient enemy's ginseng, therefore named sea cucumber.Sea cucumber, which has, improves memory, delays sexual gland aging, prevents artery sclerosis and resists swollen
Knurl etc. acts on.With the popularization of sea cucumber value knowledge, sea cucumber progresses into common people's dining table.Living environment determines sea cucumber quality.
With the continuous improvement of sea cucumber economic value, the area of China's holothruian cultures is consequently increased, and artificial at present foster
Grow and the problems such as cost is high, yields poorly be present.Nutrition arrangement is unbalanced in feed, it is impossible to meets the growth demand of sea cucumber, reduces
The yield of sea cucumber.In breeding process, many culturists are in order to improve sea cucumber survival rate, the addition added in holothurian feed
Agent contains different antibiotic and hormone, so not only influences the quality of sea cucumber, and also brings one to the body of eater
Fixed harm.In the market lacks the sea cucumber compound feed of high-quality and safety, and the feeding quality used in the raiser of part is inferior, influences sea
The quality of ginseng.
Selenium is a kind of fabulous anti-peroxidation material, to lacking albumen as one of 15 kinds of nutrients needed by human
It is malnutritive caused by matter also to there is preferable healing to act on, human body selenium intake deficiency, slight and moderate influence or unobvious, but
Severe, which lacks, easily causes the diseases such as angiocardiopathy, diabetes, hypertension.Conventional selenium-supply is mainly by with sodium selenite
Based on inorganic selenium supplemented, but sodium selenite safety using amount is not easy to hold, and easily causes human body to be poisoned, as people are to selenium
Progressively going deep into for research, by food selenium-supply, turns into a kind of most health and safety and the selenium-supply mode being easily accepted.
The content of the invention
In order to solve the above problems, the invention provides a kind of holothurian culture method, and sea is fed by using Se-enriched feedstuff
Ginseng, not only aquaculture cost is low for this method, yield is high, and nutritive value enriches, and whole breeding process is environmentally friendly, nuisanceless, production
The finished product selenium-enriched sea cucumber gone out can reach organic standard.
The present invention can be achieved through the following technical solutions：
A kind of holothurian culture method, comprises the following steps：
(1) water transparency 1-1.5m in culturing pool is controlled, 20-25 DEG C of water temperature, adjusts water body pH7.5-8.5, dissolved oxygen
More than 5mg/L, changes water every night 1 time, changes water 1/3-1/2 every time, and it is 5-7% to keep immunological regulation agent concentration in water body；
(2) young ginseng period, sargassum thunbergii is fed daily and grinds liquid 2 times, each ten thousand/ml of 20-30；
(3) sea cucumber forms later stage increase plus selenium feed, and day feeding volume 5-10% is daily 1-2 times.
Upper step (1) immunomodulator：Probiotics preparation 2-2.4 parts, CHAGA extract 0.2-0.4 parts, diseased young shoot of Omei mountain bamboo
15-17 parts, curcuma zedoary 16-18 Fen, indian rorippa herb 8-14 parts, banksia rose 10-14 parts, Radix Angelicae Sinensis 6-8 parts, malt 6-10 parts, hawthorn 9-13 parts, Cape jasmine
Sub- 10-16 parts, yellowish-white 17-19 parts, Radix Astragali 12-18 parts, honeysuckle 4-6 parts, dried orange peel 6-8 parts, radix glycyrrhizae 12-16 parts；It is described immune
The preparation method of conditioning agent is that each traditional Chinese medicine ingredients are boiled into 5-7h, and probiotics preparation and CHAGA extract are added after cooling, is filled
Divide mixing.
The probiotics preparation includes bacillus subtilis, lactic acid bacteria, nitrobacteria；The technique of the probiotics preparation
It is 150 DEG C, feed rate 50r/min in EAT, bacterium solution is 1 with protective agent proportioning to be:Sprayed under conditions of 1
Drying prepares probiotics preparation；The composite protectant is 10% beta-schardinger dextrin and 10% fructus hordei germinatus leaching powder by 1:1 ratio mixing.
The CHAGA extract：It is 1 to take CHAGA powder to add solid-liquid ratio:50 TBS is as Extraction buffer, regulation
PH is 8, extracts 20h, filters to take filtrate drying；The TBS is pH7.2,0.01mol/L Tris-HCl, containing 0.15mol/L
NaCl。
Upper step (3) plus selenium feed：Sodium humate, Hu pummelo peel extract 11-13 parts, degumming Kelp Powder 31-39 parts, horse hair
Algae powder 15-16 parts, scallop digested tankage 6-7 parts, shrimp shell meal 9-11 parts, medical stone powder 11-13 parts, squid visceral meal 2-4 parts, oyster shell whiting
0.8-1.2 parts, sweet potato leaves powder 12-18 parts, sea lettuce 1.2-1.8 parts, palm oil 0.3-0.5 parts, light-coloured vinegar 1-1.5 parts, selenium methionine
0.06-0.08 parts；The sodium humate usage amount is 5-10g/66700m3。
The Hu pummelo peel extract：Hu shaddock ped drying and crushing is taken, crosses 60 mesh sieves, adds 0.4% amylase, regulation pH is 6,
55min is handled in 60 DEG C of water bath chaders, 1mol/L NaOH, the alkaline hydrolysis in 40 DEG C of water bath chaders are added after enzymolysis
60min, then washed with clear water to neutrality, take filtrate to dry after filtering.
The processing technology of described plus selenium feed is：By each raw material meal is broken be sufficiently mixed after, be put into powder in micronizer
80 mesh are broken to, particle is made through aquatic feeds grit granulator, 200-300 mesh is crushed again after curing, drying；The feeding
Material and sea cucumber weight ratio are 1:50-100.
1000 tails of siganus guttatus/66700m is cultivated in culturing pool3, suspend 20-22kg/66700m in midair3Marine sponge Hymeniacidon perleve.
The present invention possesses following beneficial effect：
The present invention relates to a kind of holothurian culture method, the probiotics preparation in immunomodulator can improve sea cucumber body and exempt from
Epidemic disease power, strengthen digestive system enzymatic activity, prevent and cure diseases and promote to grow；Water body environment can be improved, suppresses the breeding of water extraction harmful bacteria
Growth, improve sea cucumber body antibacterial defence activity；The colony balance of aquatic products sea cucumber enteron aisle can be effectively adjusted, adjusts body intestinal mucosa
Immunocompetence, improve survival rate of sea cucumber when being attacked by disease；It is common that sea cucumber side rot disease, rotten stomach trouble etc. can effectively be reduced
The incidence of disease of disease；Probiotics preparation after spray drying more stable can enduringly be played effectiveness；
Immunomodulator of the present invention adds CHAGA extract, and sea cucumber foreign immunologic point is used as by the use of CHAGA extract
Son, the function of its immune system can be strengthened, avoid the generation of pest and disease damage；
The present invention adds the organic macromolecule polymer that the sodium humate in selenium feed is alkalescence, can be inhaled in holothruian cultures
Poisonous and harmful substance in attached water environment, purifying aquaculture environment, moreover it is possible to as nutriment, containing abundant protein, have
The bioactive substances such as machine calcium, trace element, disease-resistant factor, be advantageous to conversion of the sea cucumber to nutriment and absorb and new old generation
Thank, it is possible to reduce the generation of intestines problem；
The addition of Hu pummelo peel extract has facilitation to sea cucumber production in feed of the present invention, is produced in sea cucumber body each
Kind digestive ferment and vitamin, significantly improve effective nutrient content of feed；Containing bioactive substance, food conversion can be improved
Rate, strengthen the Nutrition and Metabolism in sea cucumber body；It is good immunoactivator, intestine immunity organ growth can be stimulated to develop, enhancing
Body's immunity；Promote absorbing for calcium, phosphorus and trace element；
Basket fish is raised together in holothurian cultivation pond of the present invention, both can control the procreation growth of the algae such as sedge, and can prevention
Cultivate species disease to occur, play a part of " street cleaner ", reduce the use of medicine, supported material is thus formed nuisanceless, healthy
The new model grown, greatly promote the yield and quality of the aquaculture organisms such as sea cucumber；
Marine sponge Hymeniacidon perleve is added in sea cucumber culture pond of the present invention, filter feeder sponge has special immunologic mechanism,
Pathogen in drinking water environment can be largely filtered, therefore sponge can effectively control pathogen contamination in breeding water body；
The present invention uses this selenium-rich cultural method, and without any toxicity and side effects, not only Se content is high, while nutrition for the sea cucumber of production
Value is high, meets modern to health and the dual requirementses of nutrition, in addition, improving the disease resistance ability of sea cucumber, survival rate is high.
Embodiment
In order that the technical means, the inventive features, the objects and the advantages of the present invention are easy to understand, tie below
Specific embodiment is closed, the present invention is expanded on further.
Embodiment 1
A kind of holothurian culture method, is mainly included the following steps that：
(1) control culturing pool in water transparency 1.25m, 22.5 DEG C of water temperature, adjust water body pH=8, dissolved oxygen 5mg/L with
On, change water every night 1 time, change water 5/12 every time, it is 6% to keep immunological regulation agent concentration in water body；
(2) young ginseng period, sargassum thunbergii is fed daily and grinds liquid 2 times, every time 250,000/mL；
(3) sea cucumber form the later stage increase plus selenium feed, day feeding volume 7.5%, 2 times a day.
A kind of holothurian culture method of the present embodiment, step (1) immunomodulator are：2.2 parts of probiotics preparation, white birch
Fine and soft 0.3 part of extract, 16 parts of diseased young shoot of Omei mountain bamboo, 11 parts of the Fen, indian rorippa herbs of curcuma zedoary 17,12 parts of the banksia rose, 7 parts of Radix Angelicae Sinensis, 8 parts of malt, hawthorn 11
Part, 13 parts of cape jasmine, yellowish-white 18 parts, 15 parts of the Radix Astragali, 5 parts of honeysuckle, 7 parts of dried orange peel, 14 parts of radix glycyrrhizae；The preparation of the immunomodulator
Method is that each traditional Chinese medicine ingredients are boiled into 6h, and probiotics preparation and CHAGA extract are added after cooling, is sufficiently mixed.
Above-mentioned probiotics preparation includes bacillus subtilis, lactic acid bacteria, nitrobacteria；The technique of the probiotics preparation
It is 150 DEG C, feed rate 50r/min in EAT, bacterium solution is 1 with protective agent proportioning to be:Sprayed under conditions of 1
Drying prepares probiotics preparation；The composite protectant is 10% beta-schardinger dextrin and 10% fructus hordei germinatus leaching powder by 1:1 ratio mixing.
The preparation of above-mentioned CHAGA extract：It is 1 to take CHAGA powder to add solid-liquid ratio:50 TBS buffers as extraction
Liquid, regulation pH are 8, extract 20h, filter to take filtrate drying；The TBS is pH=7.2,0.01mol/L Tris-HCl, is contained
0.15mol/LNaCl。
A kind of holothurian culture method of the present embodiment, the step (3) plus selenium feed are：Sodium humate, Hu pummelo peel extract
12 parts, 35 parts of degumming Kelp Powder, 15.5 parts of sargassum powder, 6.5 parts of scallop digested tankage, 10 parts of shrimp shell meal, 12 parts of medical stone powder, cuttlefish
3 parts of visceral meal, 1 part of oyster shell whiting, 15 parts of sweet potato leaves powder, 1.5 parts of sea lettuce, 0.4 part of palm oil, 1.25 parts of light-coloured vinegar, selenium methionine
0.07 part；The sodium humate usage amount is 7.5g/66700m3。
The preparation of above-mentioned Hu pummelo peel extract：Hu shaddock ped drying and crushing is taken, crosses 60 mesh sieves, adds 0.4% amylase, regulation
PH is 6, and 55min is handled in 60 DEG C of water bath chaders, and 1mol/L NaOH, the alkali in 40 DEG C of water bath chaders are added after enzymolysis
60min is solved, is then washed with clear water to neutrality, takes filtrate to dry after filtering.
The processing technology of above-mentioned plus selenium feed is：By each raw material meal is broken be sufficiently mixed after, be put into powder in micronizer
80 mesh are broken to, particle is made through aquatic feeds grit granulator, 250 mesh are crushed again after curing, drying；The feed and
Sea cucumber weight ratio is 1:75.
A kind of holothurian culture method of the present embodiment, including cultivated in culturing pool siganus guttatus 1000 tail/
66700m3, suspend 21kg/66700m in midair3Marine sponge Hymeniacidon perleve.
Embodiment 2
A kind of holothurian culture method, is mainly included the following steps that：
(1) water transparency 1m in culturing pool is controlled, 20 DEG C of water temperature, adjusts water body pH=7.5, more than dissolved oxygen 5mg/L,
Change water every night 1 time, change water 1/3 every time, it is 5% to keep immunological regulation agent concentration in water body；
(2) young ginseng period, sargassum thunbergii is fed daily and grinds liquid 2 times, every time 200,000/mL；
(3) sea cucumber form the later stage increase plus selenium feed, day feeding volume 5%, 1 time a day.
A kind of holothurian culture method of the present embodiment, step (1) immunomodulator are：2 parts of probiotics preparation, CHAGA
0.2 part of extract, 15 parts of diseased young shoot of Omei mountain bamboo, 8 parts of the Fen, indian rorippa herbs of curcuma zedoary 16,10 parts of the banksia rose, 6 parts of Radix Angelicae Sinensis, 6 parts of malt, 9 parts of hawthorn, Cape jasmine
10 parts of son, yellowish-white 17 parts, 12 parts of the Radix Astragali, 4 parts of honeysuckle, 6 parts of dried orange peel, 12 parts of radix glycyrrhizae；The preparation method of the immunomodulator
It is that each traditional Chinese medicine ingredients are boiled into 5h, probiotics preparation and CHAGA extract is added after cooling, is sufficiently mixed.
Above-mentioned probiotics preparation includes bacillus subtilis, lactic acid bacteria, nitrobacteria；The technique of the probiotics preparation
It is 150 DEG C, feed rate 50r/min in EAT, bacterium solution is 1 with protective agent proportioning to be:Sprayed under conditions of 1
Drying prepares probiotics preparation；The composite protectant is 10% beta-schardinger dextrin and 10% fructus hordei germinatus leaching powder by 1:1 ratio mixing.
The preparation of above-mentioned CHAGA extract：It is 1 to take CHAGA powder to add solid-liquid ratio:50 TBS buffers as extraction
Liquid, regulation pH are 8, extract 20h, filter to take filtrate drying；The TBS is pH=7.2,0.01mol/L Tris-HCl, is contained
0.15mol/LNaCl。
A kind of holothurian culture method of the present embodiment, the step (3) plus selenium feed are：Sodium humate, Hu pummelo peel extract
11 parts, 31 parts of degumming Kelp Powder, 15 parts of sargassum powder, 6 parts of scallop digested tankage, 9 parts of shrimp shell meal, 11 parts of medical stone powder, cuttlefish internal organ
2 parts of powder, 0.8 part of oyster shell whiting, 12 parts of sweet potato leaves powder, 1.2 parts of sea lettuce, 0.3 part of palm oil, 1 part of light-coloured vinegar, 0.06 part of selenium methionine；
The sodium humate usage amount is 5g/66700m3。
The preparation of above-mentioned Hu pummelo peel extract：Hu shaddock ped drying and crushing is taken, crosses 60 mesh sieves, adds 0.4% amylase, regulation
PH is 6, and 55min is handled in 60 DEG C of water bath chaders, and 1mol/L NaOH, the alkali in 40 DEG C of water bath chaders are added after enzymolysis
60min is solved, is then washed with clear water to neutrality, takes filtrate to dry after filtering.
The processing technology of above-mentioned plus selenium feed is：By each raw material meal is broken be sufficiently mixed after, be put into powder in micronizer
80 mesh are broken to, particle is made through aquatic feeds grit granulator, 200 mesh are crushed again after curing, drying；The feed and
Sea cucumber weight ratio is 1:50.
A kind of holothurian culture method of the present embodiment, including cultivated in culturing pool siganus guttatus 1000 tail/
66700m3, suspend 20kg/66700m in midair3Marine sponge Hymeniacidon perleve.
Embodiment 3
A kind of holothurian culture method, is mainly included the following steps that：
(1) control culturing pool in water transparency 1.5m, 25 DEG C of water temperature, adjust water body pH=8.5, dissolved oxygen 5mg/L with
On, change water every night 1 time, change water 1/2 every time, it is 7% to keep immunological regulation agent concentration in water body；
(2) young ginseng period, sargassum thunbergii is fed daily and grinds liquid 2 times, every time 300,000/mL；
(3) sea cucumber form the later stage increase plus selenium feed, day feeding volume 10%, 2 times a day.
A kind of holothurian culture method of the present embodiment, step (1) immunomodulator are：2.4 parts of probiotics preparation, white birch
Fine and soft 0.4 part of extract, 17 parts of diseased young shoot of Omei mountain bamboo, 14 parts of the Fen, indian rorippa herbs of curcuma zedoary 18,14 parts of the banksia rose, 8 parts of Radix Angelicae Sinensis, 10 parts of malt, hawthorn 13
Part, 16 parts of cape jasmine, yellowish-white 19 parts, 18 parts of the Radix Astragali, 6 parts of honeysuckle, 8 parts of dried orange peel, 16 parts of radix glycyrrhizae；The preparation of the immunomodulator
Method is that each traditional Chinese medicine ingredients are boiled into 7h, and probiotics preparation and CHAGA extract are added after cooling, is sufficiently mixed.
Above-mentioned probiotics preparation includes bacillus subtilis, lactic acid bacteria, nitrobacteria；The technique of the probiotics preparation
It is 150 DEG C, feed rate 50r/min in EAT, bacterium solution is 1 with protective agent proportioning to be:Sprayed under conditions of 1
Drying prepares probiotics preparation；The composite protectant is 10% beta-schardinger dextrin and 10% fructus hordei germinatus leaching powder by 1:1 ratio mixing.
The preparation of above-mentioned CHAGA extract：It is 1 to take CHAGA powder to add solid-liquid ratio:50 TBS buffers as extraction
Liquid, regulation pH are 8, extract 20h, filter to take filtrate drying；The TBS is pH=7.2,0.01mol/L Tris-HCl, is contained
0.15mol/LNaCl。
A kind of holothurian culture method of the present embodiment, the step (3) plus selenium feed are：Sodium humate, Hu pummelo peel extract
13 parts, 39 parts of degumming Kelp Powder, 16 parts of sargassum powder, 7 parts of scallop digested tankage, 11 parts of shrimp shell meal, 13 parts of medical stone powder, cuttlefish internal organ
4 parts of powder, 1.2 parts of oyster shell whiting, 18 parts of sweet potato leaves powder, 1.8 parts of sea lettuce, 0.5 part of palm oil, 1.5 parts of light-coloured vinegar, selenium methionine 0.08
Part；The sodium humate usage amount is 10g/66700m3。
The preparation of above-mentioned Hu pummelo peel extract：Hu shaddock ped drying and crushing is taken, crosses 60 mesh sieves, adds 0.4% amylase, regulation
PH is 6, and 55min is handled in 60 DEG C of water bath chaders, and 1mol/L NaOH, the alkali in 40 DEG C of water bath chaders are added after enzymolysis
60min is solved, is then washed with clear water to neutrality, takes filtrate to dry after filtering.
The processing technology of above-mentioned plus selenium feed is：By each raw material meal is broken be sufficiently mixed after, be put into powder in micronizer
80 mesh are broken to, particle is made through aquatic feeds grit granulator, 300 mesh are crushed again after curing, drying；The feed and
Sea cucumber weight ratio is 1:100.
A kind of holothurian culture method of the present embodiment, including cultivated in culturing pool siganus guttatus 1000 tail/
66700m3, suspend 22kg/66700m in midair3Marine sponge Hymeniacidon perleve.
Comparative example 1
Difference with embodiment 1 is, does not increase CHAGA extract in immunomodulator.
Comparative example 2
Difference with embodiment 1 is, not in the pond culture siganus guttatus of culturing sea cucumbers.Comparative example 3
Difference with embodiment 1 is, marine sponge Hymeniacidon perleve is not suspended in midair carried out in the culturing pool water body of sea cucumber cofferdam it is auxiliary
Help to foster and grow.
Comparative example 4
Difference with embodiment 1 is, adds and does not add Hu pummelo peel extract in selenium feed.
Effect assessment
Culturing pool makes：Using breadth 8m, after the woven cloth sewing in flakes that length is equal with pond, tile bottom of pond, uses stone
Block is pushed down；The fritter that 5cm × 8cm is cut into waste foam does buoy and floats one side of woven cloth, turns into wall, height 1m,
Wall and wall distance 4m, the direction of wall are parallel with drainage gate；In wall spacing, it is each to make profile with woven cloth
The artificial reef of different " heaving of the sea ", bottom dropstone.
Method according to above-described embodiment 1~3, comparative example 1~4 is cultivated, and the data recorded are needed in breeding process
Represented with form, it is as a result as follows：
As can be known from the above table, the gap between embodiment 1~3 is not very big, and the data that each side is shown are equal
It is better than comparative example；
In above-mentioned project, the infection disease rate of descent of sea cucumber has close relationship with four below, and infection rate of descent is higher,
Rate of body weight gain, protein content, calcium content are also higher, and death and culling rate is just smaller, illustrates that sea cucumber is more healthy, and development is better in vivo；
Comparing embodiment 1 and comparative example 1~4, CHAGA extract in embodiment 1 as sea cucumber foreign immunologic molecule,
By being combined instead of autologous immune molecule with the carbohydrate-binding protein of phage surface, strengthen the function of its immune system, so as to
Reduce disease pest infection；Unlike comparative example 2, embodiment 1 raises together the algae such as basket fish, control sedge in holothurian cultivation pond
The procreation growth of class, prevents algae from rotting to influence water quality, occurs so as to prevent to cultivate species disease, greatly promotes the cultivation such as sea cucumber
The yield and quality of biology；Compare comparative example 3, the filter feeder sponge in embodiment 1 has special immunologic mechanism, can be with
Pathogen such as kinds of pathogenic vibrio and excrement colibacillus group etc. in a large amount of filter drinking water environment, therefore sponge effectively can be controlled in breeding water body
Pathogen contamination；And finally except providing nutrient and active material, it is still immunized the Hu pummelo peel extract of the shortage of comparative example 4
Activator, intestine immunity organ growth can be stimulated to develop, improve sea cucumber antibody level and macrophage activity, strengthen body body
Liquid is immunized and cellular immune function, nips in the bud and immerses internal pathogenic bacteria, prevents the generation of disease, be remarkably decreased the dead of sea cucumber
The rate of washing in a pan.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more specific and detailed, but simultaneously
Therefore the limitation to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the guarantor of the present invention
Protect scope.
Claims (8)
1. a kind of holothurian culture method, it is characterised in that comprise the following steps：
(1) water transparency 1-1.5m in culturing pool is controlled, 20-25 DEG C of water temperature, adjusts water body pH7.5-8.5, dissolved oxygen 5mg/L
More than, change water every night 1 time, change water 1/3-1/2 every time, it is 5-7% to keep immunological regulation agent concentration in water body；
(2) young ginseng period, sargassum thunbergii is fed daily and grinds liquid 2 times, each ten thousand/ml of 20-30；
(3) sea cucumber forms later stage increase plus selenium feed, and day feeding volume 5-10% is daily 1-2 times.
A kind of 2. holothurian culture method according to claim 1, it is characterised in that step (1) immunomodulator：Benefit
Probiotics preparation 2-2.4 parts, CHAGA extract 0.2-0.4 parts, diseased young shoot of Omei mountain bamboo 15-17 parts, curcuma zedoary 16-18 Fen, indian rorippa herb 8-14 parts,
Banksia rose 10-14 parts, Radix Angelicae Sinensis 6-8 parts, malt 6-10 parts, hawthorn 9-13 parts, cape jasmine 10-16 parts, yellowish-white 17-19 parts, Radix Astragali 12-18
Part, honeysuckle 4-6 parts, dried orange peel 6-8 parts, radix glycyrrhizae 12-16 parts；The preparation method of the immunomodulator is, by each traditional Chinese medicine ingredients
5-7h is boiled, probiotics preparation and CHAGA extract are added after cooling, is sufficiently mixed.
3. a kind of holothurian culture method according to claim 2, it is characterised in that the probiotics preparation includes withered grass bud
Spore bacillus, lactic acid bacteria, nitrobacteria；The technique of the probiotics preparation is, is 150 DEG C in EAT, feed rate is
50r/min, bacterium solution are 1 with protective agent proportioning:Spray drying is carried out under conditions of 1 and prepares probiotics preparation；The complex protection
Agent is 10% beta-schardinger dextrin and 10% fructus hordei germinatus leaching powder by 1:1 ratio mixing.
A kind of 4. holothurian culture method according to claim 2, it is characterised in that the CHAGA extract：Take white birch
It is 1 that fine and soft powder, which adds solid-liquid ratio,:50 TBS is 8 as Extraction buffer, regulation pH, extracts 20h, filters to take filtrate drying；Institute
It is pH7.2,0.01mol/L Tris-HCl, NaCl containing 0.15mol/L to state TBS.
5. a kind of holothurian culture method according to claim 1, it is characterised in that the step (3) plus selenium feed：Corruption is planted
Sour sodium, Hu pummelo peel extract 11-13 parts, degumming Kelp Powder 31-39 parts, sargassum powder 15-16 parts, scallop digested tankage 6-7 parts, shrimp shell
Powder 9-11 parts, medical stone powder 11-13 parts, squid visceral meal 2-4 parts, oyster shell whiting 0.8-1.2 parts, sweet potato leaves powder 12-18 parts, sea lettuce
1.2-1.8 parts, palm oil 0.3-0.5 parts, light-coloured vinegar 1-1.5 parts, selenium methionine 0.06-0.08 parts；The sodium humate usage amount
For 5-10g/66700m3。
A kind of 6. holothurian culture method according to claim 5, it is characterised in that the Hu pummelo peel extract：Take Hu shaddock
Skin drying and crushing, 60 mesh sieves are crossed, add 0.4% amylase, regulation pH is 6, and 55min, enzyme are handled in 60 DEG C of water bath chaders
1mol/L NaOH, the alkaline hydrolysis 60min in 40 DEG C of water bath chaders is added after solution, is then washed with clear water to neutrality, after filtering
Filtrate is taken to dry.
A kind of 7. holothurian culture method according to claim 5, it is characterised in that described plus selenium feed processing technology
It is：By each raw material meal is broken be sufficiently mixed after, be put into micronizer and be crushed to 80 mesh, through aquatic feeds grit granulator
Particle is made, 200-300 mesh is crushed again after curing, drying；The feed and sea cucumber weight ratio are 1:50-100.
8. a kind of holothurian culture method according to claim 1, it is characterised in that siganus guttatus is cultivated in culturing pool
1000 tails/66700m3, suspend 20-22kg/66700m in midair3Marine sponge Hymeniacidon perleve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710824839.0A CN107494354A (en) | 2017-09-12 | 2017-09-12 | A kind of holothurian culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710824839.0A CN107494354A (en) | 2017-09-12 | 2017-09-12 | A kind of holothurian culture method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107494354A true CN107494354A (en) | 2017-12-22 |
Family
ID=60695407
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710824839.0A Withdrawn CN107494354A (en) | 2017-09-12 | 2017-09-12 | A kind of holothurian culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107494354A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114631596A (en) * | 2020-12-15 | 2022-06-17 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Special paste bait for sea cucumber auricular larvae |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102084837A (en) * | 2010-12-24 | 2011-06-08 | 营口市实发参蜇产业有限公司 | Sea cucumber seedling culture method |
| CN102100195A (en) * | 2010-12-20 | 2011-06-22 | 山东海益宝水产股份有限公司 | Method for breeding apostichopus japonicus selenka |
| CN102771674A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Organic selenium-rich sea cucumber feed |
| CN106259066A (en) * | 2016-08-05 | 2017-01-04 | 山东省海洋资源与环境研究院 | A kind of cultural method of selenium-enriched sea cucumber |
| CN106332814A (en) * | 2016-08-19 | 2017-01-18 | 青岛润物源生物科技有限公司 | Fully-ecological holothurian cultivation method |
-
2017
- 2017-09-12 CN CN201710824839.0A patent/CN107494354A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100195A (en) * | 2010-12-20 | 2011-06-22 | 山东海益宝水产股份有限公司 | Method for breeding apostichopus japonicus selenka |
| CN102084837A (en) * | 2010-12-24 | 2011-06-08 | 营口市实发参蜇产业有限公司 | Sea cucumber seedling culture method |
| CN102771674A (en) * | 2012-08-15 | 2012-11-14 | 苏州硒谷科技有限公司 | Organic selenium-rich sea cucumber feed |
| CN106259066A (en) * | 2016-08-05 | 2017-01-04 | 山东省海洋资源与环境研究院 | A kind of cultural method of selenium-enriched sea cucumber |
| CN106332814A (en) * | 2016-08-19 | 2017-01-18 | 青岛润物源生物科技有限公司 | Fully-ecological holothurian cultivation method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114631596A (en) * | 2020-12-15 | 2022-06-17 | 大连鑫玉龙海洋生物种业科技股份有限公司 | Special paste bait for sea cucumber auricular larvae |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102150757B (en) | Aquatic product feed additive composition, preparation method and application thereof | |
| CN103704530B (en) | A kind of loach seedling special compound feed | |
| CN104857337A (en) | Fermented traditional Chinese medicine microbial preparation for parent grass carp and preparation method of microbial preparation | |
| CN107439437A (en) | A kind of Urechis uniconctus cultural method | |
| CN103891653A (en) | Pond ecological aquaculture technology of new species of Murray cods | |
| CN104522416B (en) | A kind of the juvenile fish pellet and preparation method of China snakehead | |
| CN102783439A (en) | Takifugu obscurus three-stage type culture process | |
| CN108201025A (en) | A kind of multifilament cray feed and preparation method thereof | |
| CN105284675A (en) | Biological bait preparation method for culturing mud fishes | |
| CN107348229A (en) | A kind of catfish feed eating belt agent and preparation method thereof | |
| CN105210938A (en) | A kind of loach microorganism cultivation method | |
| CN109362983A (en) | A kind of selenium-rich chicken feed and preparation method thereof | |
| KR20110099872A (en) | A feed additive for raising fishes and a method of manufacturing for a feed for raising fishes | |
| CN108739562A (en) | A kind of abalone breeding method | |
| CN107361243A (en) | A kind of feed addictive for promoting cold water fish growth and application thereof | |
| CN104585516A (en) | Production method of immunocompetence feed for river crabs | |
| CN106721525A (en) | A kind of digestive utilization ratio catfish feed high | |
| CN106805007A (en) | A kind of high yield catfish feed eating belt agent and preparation method thereof | |
| CN107494354A (en) | A kind of holothurian culture method | |
| CN105192311A (en) | Livestock and poultry compound feed additive | |
| CN104757311B (en) | A kind of fish feed additive and preparation method thereof | |
| CN103875991B (en) | A kind of gosling mixed feed and preparation method thereof | |
| CN106819617A (en) | A kind of rheum officinale fish meal of growth promotion diseases prevention and its application | |
| CN106072088A (en) | Thallus Laminariae (Thallus Eckloniae) Ganoderma mycelium is prepared for culture medium with Thallus Laminariae (Thallus Eckloniae) | |
| CN105248317A (en) | Method for improving water quality in loach cultivation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171222 |
|
| WW01 | Invention patent application withdrawn after publication |