CN107348229A

CN107348229A - A kind of catfish feed eating belt agent and preparation method thereof

Info

Publication number: CN107348229A
Application number: CN201611217954.3A
Authority: CN
Inventors: 田岗; 邢晓慧
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-12-26
Filing date: 2016-12-26
Publication date: 2017-11-17

Abstract

The invention discloses a kind of catfish feed eating belt agent and preparation method thereof, belong to feed addictive preparing technical field, based on catfish nutritional need and water body environment, with reference to its characteristic of ingesting, optimizing raw material formula, science compounding is greatly improved palatability and efficiency of feed utilization, has the composite flavor peptide for compared with strong anti-oxidation effect, eliminating free radical；Cultures of S. cerevisiae with functional characteristics such as powerful trophism, bioactivity and inoxidizability；Calcium, iron and Soluble Fiber, the calcium berry extract that there is powerful embedding effect, extend the shelf life can effectively be supplemented；Cryoprotective effects and stability are remarkably improved, improve water resistance and glossiness, enhancing and promotion palatability, reduce the wormwood seed powder of the rate containing powder；Abundant nutrition material, bioactive substance, fragrance matter and the Wheat Sprout Powder in phytoenzyme source can be provided；Finally prepare the catfish feed eating belt agent that a kind of palatability is strong, production performance is good.

Description

A kind of catfish feed eating belt agent and preparation method thereof

Technical field

The present invention relates to feed addictive, and in particular to a kind of catfish feed eating belt agent and preparation method thereof.

Background technology

The feeding behaviour of fish as other animal populations, by search for, position, receive, obtain etc. it is a series of Quality of food evaluation procedure, then by the process such as swallowing, digesting, absorbing and assimilating, this is directly connected to energy requirement, again The growing of fish, ripe and egg laying amount existence extrusion are decide, the feeding behaviour of fish is related to many sensory systems, still What is played a major role is two big sensory systems：Olfactory system and gustatory system, the feeding behaviour of fish can be divided into 2 stages： 1) perception of the olfactory system to the direction and position of food, it is produced close after interest；2) gustatory system is to the food sense of taste Evaluation, and finally decide whether to feed, in addition, various physical stimulation sense organs also play crucial work to the palatability of feed With.In a word, smell and gustatory system feel to play main function to the food calling composition of fish in the feeding process of fish, exploitation with It can reasonably be played an important role with protection water body environment using growth of the Feeding-attractants of Fish to aquatic fish.

Catfish is animal feeding fish, is the improved seeds in fresh-water fishes, and its is nutritious, aqueous in every 100 grams of flesh of fish Point 64.1 grams, 14.4 grams of protein, and contain several mineral materials and trace element, be particularly suitable for it is weak it is deficient, malnutritive it People eats.In addition to the roe of catfish has miscellaneous taste inedibility, whole body is precious, and catfish is rare nutrient excellent product, early in history records In just it is on the books, can be compared favourably with shark's fin, wild soft-shelled turtle, be fish in treasure.Its dietary function and medical value is other Not available for fish, strong able-bodied bone and the effect of lengthening one's life are the characteristics of it are unique.Simultaneously catfish be lactagogue good merchantable brand, its sweet property Temperature, and play the role of nourishing yin and nourishing blood, bowl spares gas, appetizing, diuresis, suitable common people eat, especially with old, young, women's postpartum and The bad people of digestive function is applicable the most, has wide market prospects and development potentiality, turns into raiser's concern in recent years Focus.

At present, patent disclosed in related catfish feed eating belt agent is more：The B of Chinese patent CN 103053842 disclose one Kind catfish food calling nutritional agents, its composition include fresh fish slurry, spiral shell freshwater mussel meat slurry, earthworm powder, yellow meal worm powder, spirulina powder, dusty yeast, pig Liver xeraphium, EM bacterium, wheat-middlings, wheat-middlings are matrix, and various solid materials crush respectively, and trash fish is milled to fish slurry, and spiral shell freshwater mussel, which is shelled, to be taken Meat wears into meat slurry, and various raw material powder, slurry are sieved with 100 mesh sieve tulles, according to proportioning be put into fodder mixing machine stirring 30- 50 minutes, puddle uniformly, stored after air-drying, made an addition in 15% -25% ratio in catfish feed during use；The invention is matched somebody with somebody Fang Xuanyong a variety of fresh animal raw material, animal powder, spirulina, yeast and microorganism formulations, smell is unique, attractant is strong, right In the conversion feed of catfish, catfish domestication food calling, newly putting the feedstuff feeding of pond catfish in a suitable place to breed has good attractant effect, and also High-quality animal protein and various nutritional ingredients can be provided for catfish.

A kind of catfish baits of the A of Chinese patent CN 104430233 and preparation method thereof.Specifically include：The bait is by Nian Fish meal, pluck, sucrose, sheep blood are combined and formed through everfermentation.Raw material easily obtains used by the invention, and valency Lattice are cheap, and the bait prepared has one special fragrance, can be good at attracting catfish to take bait, then reach what is risen to the bait Purpose.Meanwhile the prepared bait cost out of the invention is low, application effect is notable, is worth promoting.

The A of Chinese patent CN 104543450 disclose a kind of fermentation catfish feed, are made from the following raw materials in parts by weight： Soy noodle 80-90, sunflower meal 20-25, corn protein powder 6-8, red date powder 10-14, oyster meat 10-15, squid chip 5-10, bean curd Newborn 13-17, peach kernel 5-7, corn stem leaf 10-12, lemon 13-16, flower of Panax ginseng 1-2, palma 2-3, leek 2-3, yolk powder 7-9, butter 3-4, saccharomycete 2-3, phagostimulant 3-4, appropriate amount of water；The fermentation catfish feed of the invention, the oyster meat of addition, squid Piece is rich in multiple nutritional components such as the several mineral materials such as protein, calcium.The lemon of addition can play antibacterial, heat-clearing, promote appetite Effect.Obtained catfish feed is rich in various protein, fat, vitamin and mineral needed for catfish, and proportioning is suitable, accelerates The growth of catfish, improve immunity of organisms and tissue metabolism's ability.

The A of Chinese patent CN 103704485 disclose a kind of clarias fuscus feed and preparation method thereof, by following parts by weight Raw material be made：Ripe pig blood 45-50, minced fillet 12-15, fish meal 9-12, wheat bran 24-28, bean dregs 12-15, vinasse 5-6, rapeseed Cake 12-15, apple leaf 8-10, gynostemma pentaphylla 3-4, spermacoce latifolia 1-2, dandelion 2-3, twocolor sealavander herb 2-3, folium lycii 4-5, rhizoma zingiberis 1-2, root of kudzu vine 10-12, phagostimulant 3-4, feed fermentation agent 1-2.Contain phagostimulant in the invention feed formula, the food of fish can be improved It is intended to, adds appetite, quick weightening, meanwhile, the composition such as dandelion, gynostemma pentaphylla in formula, can heat-clearing solution beneficial to health Poison, after clarias fuscus fishes are edible, human body is passed into by food chain, also beneficial to health.

The B of Chinese patent CN 103598443 disclose a kind of catfish feed, the feed by following parts by weight raw material system ：Corn 55-60, soybean slag 20-25, dregs of beans 15-18, maize straw 12-15, black bean powder 10-12, wheat-middlings 12-15, water earthworm Powder 6-8 parts, sheep liver powder 5-6, shrimp shell meal 4-5, laver powder 5-6, dry ferment 4-5, it is dehydrated clover powder 6-8, asparagus lettuce leaf 6-8, food Appropriate salt, phagostimulant 4-5.The catfish feed that the invention is prepared, from the omnivorous characteristic of catfish, it with the addition of water earthworm, sheep The raw materials such as liver, seaweed, asparagus lettuce, the nutritional ingredient needed for the development of catfish fast-growth is not only met, while add feed Palatability, avoid the waste of feed.

The A of Chinese patent CN 104082583 disclose the catfish feed that a kind of duckweed/corn must be compound, and its feature exists In being made from the following raw materials in parts by weight：Black rice face 17-19, small rice and flour 18-20, sorghum flour 16-18, potato juice 11-13, Glucose 4-6, duckweed 17-21, corn must 12-14, dried small shrimp 13-15, quail meat 8-10, soya bean 14-16, sesame 4-8, Moschus 0.02-0.04, monosodium glutamate 0.1-0.2, grape-kernel oil 1.2-3.2, string of bells hung round the neck grass 0.1-0.3, Radix Isatidis 0.2-0.4, ginkgo leaf 0.2- 0.5th, auxiliary agent 3-6, suitable quantity of water；Feed described in the invention is effectively compound by various ingredients, and nutrition is balanced, and addition duckweed, sweet tea are beautiful Rice must can excite the appetite of fish, have poly- fish fast, eat the characteristics of not dissipating and easy to use long, easily storage has considerable Market application foreground.

Although catfish phagostimulant species disclosed above is various, various informative, dispensing is complicated, and flavor raw material does not have mostly Extracted and reprocessed again, wastage of material is serious, and phagostimulant addition is big, and flavor active principle utilization rate is low, and catfish Palatability, digestibility, feedstuff-meat ratio, the production performance such as disease resistance it is still very undesirable.

Therefore, ingest the factors such as characteristic, digestion characteristics, nutritional need and water body environment, prepare a kind of agreeable to the taste with reference to catfish The catfish feed eating belt agent strong, that production performance is good of property is necessary.

The content of the invention

Technical problem solved by the invention is the defects of overcoming existing catfish feed eating belt agent, with catfish nutritional need and Based on water body environment, with reference to its characteristic of ingesting, optimizing raw material formula, science compounding is greatly improved palatability and feed conversion Rate, there is the composite flavor peptide for compared with strong anti-oxidation effect, eliminating free radical；With powerful trophism, bioactivity and anti-oxidant The Cultures of S. cerevisiae of the functional characteristics such as property；Calcium, iron and Soluble Fiber can be effectively supplemented, there is powerful embedding effect, prolong The calcium berry extract of long shelf-life；Cryoprotective effects and stability are remarkably improved, improve water resistance and glossiness, enhancing and promotion Palatability, reduce the wormwood seed powder of the rate containing powder；Abundant nutrition material, bioactive substance, fragrance matter and phytoenzyme can be provided The Wheat Sprout Powder in source；Finally prepare the catfish feed eating belt agent that a kind of palatability is strong, production performance is good.

In order to achieve the above object, the present invention uses following technical scheme：

A kind of catfish feed eating belt agent, mainly prepared by the raw material of following parts by weight：

100 parts of composite flavor peptide, calcium berry extract 8-12 parts, Wheat Sprout Powder 6-12 parts, glycine betaine 5-9 parts, tangerine peel powder 4-6 Part, wormwood seed powder 2-6 parts, yeast culture 2-6 parts, seawood meal 2-4 parts, EM bacteria powder 0.5-1.5 parts；

Further, the composite flavor peptide is science compounding earthworm, sheep blood and soybean protein, through high-pressure pulse electric, The low temperature processing techniques such as complex enzyme subsection enzymolysis, ultrafiltration, segmentation reaction, freeze-drying and prepare have earthworm, sheep blood and big concurrently The composite flavor flavor peptide of beans flavor, is not only greatly improved the palatability and efficiency of feed utilization of catfish feed, and with compared with Strong antioxidant effect, eliminate free radical；

Preferably, the preparation method of the composite flavor peptide, comprises the following steps：4-6 in mass ratio:2-4:1-3 is weighed Fresh earthworm, sheep blood and soybean protein, mix, rub to obtain compound, add the solion of 2-5 times of its quality, mix, regulation PH value is 6-8, at room temperature in electric-field intensity 25-35kV/cm, burst length 300-500 μ s, pulse frequency 200-300Hz bars High-pressure pulse electric processing 20-30min is carried out under part；Then mixture quality 0.04-0.08% complex enzyme is added, in 50- 60 DEG C of enzymolysis 30-50min, 35-45 DEG C of enzymolysis 40-60min is then cooled to, 90 DEG C of enzyme deactivation 10min, is down to room temperature, degerming mistake Enzymolysis liquid is filtered to obtain, enzymolysis liquid is taken the permeate between two films, be concentrated under reduced pressure into by 1000Da and 5000Da ultrafiltration membrance filter Solid content is that 18-22% must digest concentrate；Enzymolysis concentrate quality 5-7% reduced sugar is added, successively in 112-118 DEG C reaction 30-50min and 135-145 DEG C reaction 45-65min after；The milipore filter mistake that reaction solution is passed through into 1000Da and 5000Da Filter, takes the permeate between two films, and concentrate must be reacted for 26-30% by being concentrated under reduced pressure into solid content；To reaction concentrate The middle sericin peptide taken for adding its quality 0.2-0.4%, mixes, is freeze-dried and produces composite flavor peptide.

Preferably, the solion is to contain sodium ion 17-22mg/L, zinc ion 15-20mg/L, potassium ion 12- 14mg/L, calcium ion 9-11mg/L and magnesium ion the 6-10mg/L aqueous solution；

Preferably, the complex enzyme is that earthworm serine protease, alkali protease, flavor protease, lipase press matter Amount compares 4-6:2-4:0.2-0.4:0.1-0.3 is uniformly mixed；

Preferably, the reduced sugar is the one or more in xylose, ribose, glucose, fructose, galactolipin；

It is highly preferred that the quality group of the reduced sugar turns into：Xylose:Ribose:Glucose:Fructose:Galactolipin=3-5:2- 4:1-3:1-3:1-2。

Further, the calcium berry extract is with calcium, iron and higher calcium stem end, leaf of GAIGUO and the chrysanthemum of dietary fiber content Powder is raw material, through ultrasonic cleaning, biology enzyme enzymolysis, microwave drying, extrusion, ultramicro grinding and be made；Its Soluble Fiber Cellulose content is high, bioactivity is strong, has important, positive role fiber to beneficial flora in catfish body, can particularly supplement calcium Iron, the shelf-life for extending product, compared with full diet fiber, its feature, bioactivity are more powerful；

Preferably, the preparation method of the calcium berry extract, comprises the following steps：Calcium stem end, leaf of GAIGUO and inulin are pressed Mass ratio 5-9:3-6:1-3 is uniformly mixed, and is placed in supersonic wave cleaning machine and is cleaned 3-5min in 200W, 30KHz, drains, and is crushed To particle diameter 0.3-0.7mm, the water of 0.5-1.5 times of fragment quality is added, regulation pH value is 5-7, adds mixture quality 0.1- 0.3% biology enzyme, 10-15min is digested in 50-60 DEG C；It is 40-60% that enzymolysis liquid, which is concentrated under reduced pressure into solid content, is put into Microwave dryer carries out intermittent drying in 2000W, 130-150 DEG C, is allowed to moisture and reaches 4-6%, is then crushed to particle diameter 0.4-0.6mm, crushed material quality 0.2-0.4% sodium acid carbonate is added, uniformly mixing, adjustment mixture moisture is 12- 14%, room temperature, sealing and standing 2-4h, in 140-160 DEG C of screw speed 105-115r/min, temperature condition extrusion, then Ultramicro grinding produces calcium berry extract to particle diameter 0.1-0.3mm；

The biology enzyme is cellulase, laccase, dextranase, seminase, pectase 2-4 in mass ratio:1-3:1- 3:0.5-1.5:0.4-1 is uniformly mixed.

Further, the preparation method of the Wheat Sprout Powder, comprises the following steps：By wheat be placed on temperature for 10-18 DEG C, PH value is that 20-40min is soaked in 6-8 soak, and soak contains the sodium acid carbonate that mass percent concentration is 0.1-0.3% With 0.2-0.4% compound enzyme, 28-32 DEG C then is warming up to 3-5 DEG C/min speed, continues to soak 3-5h, finally with 5-7 DEG C/min speed is warming up to 50-60 DEG C of immersion 5-8min, whole immersion process is divulged information once every 15-20min, ventilation pressure 0.13-0.15MPa, while carried out in electric-field intensity 3-5kV/cm, burst length 100-300 μ s, pulse frequency 60-80Hz conditions High-pressure pulse electric processing, until wheat water content content is 40-45%；Wheat after immersion drains, and is carried out in growing floor dark Germination, dark germination temperature are kept for 20-24 DEG C, and the dark germination time is 20-24h, and wheat drying to the moisture after germination is 5-8%, low-temperature grinding, cross 60-80 mesh sieves and produce Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase, pentosanase 2-4 in mass ratio:2-4:1-3: 1-2 is uniformly mixed.

Further, the wormwood seed powder is using wormwood seed as raw material, and bigcatkin willow is aided in through high-pressure electrostatic, high-pressure pulse electric Crushed after acid soak, refrigerating processing, freeze and be made；

Preferably, the preparation method of the wormwood seed powder, comprises the following steps：By wormwood seed sabot, first in electric-field strength Spend 1-3kV/cm high-pressure electrostatics processing 5-7min；Then the soaking at room temperature 2-5h in the salicylic acid solution that concentration is 3-5mg/L, together When in electric-field intensity 2-6kV/cm, in burst length 50-100 μ s, carry out high-pressure pulse electric under the conditions of pulse frequency 100-150Hz Processing；Rinse, drain, in 3-5 DEG C stand 18-24h, then successively 1-3 DEG C refrigerate 2-4d, -3--5 DEG C freezing 1-3d, - 15--18 DEG C of freezing 10-15h, outdoor natural light is immediately placed at according to being crushed immediately after 2-4h, freezes, adds freeze-dried powder quality 3-8% sodium bicarbonate, uniformly mixing, cross 80-100 mesh sieves and produce wormwood seed powder.

Further, the yeast culture is with saccharomyces cerevisiae (Saccharomyces cerevisiae) tlj2016 Prepared to go out bacterium germination through spreading cultivation, fermenting step by step；

Preferably, the preparation method of the yeast culture, comprises the following steps：Take the saccharomyces cerevisiae after the activation of 1 ring Tlj2016 slant strains, be seeded in the 500m L triangular flasks equipped with 100m L seed culture mediums and cultivate, 28~30 DEG C, shake 25~30h is cultivated under the conditions of 150~200r/min of bed rotating speed, obtains primary seed solution；Primary seed solution is pressed into 5~10% (v/ V) inoculum concentration, it is seeded in the 500m L triangular flasks equipped with 100m L fermentation mediums and carries out fermented and cultured, fermentation time 25 ~30h, 28~30 DEG C of temperature, 150~200r/min of shaking speed, obtains secondary seed solution；By secondary seed solution with 5~10% (v/v) inoculum concentration is linked into the 5L fermentation tank ferment at constant temperature of dress 3L fermentation mediums, 28~30 DEG C of temperature, 4~6L/ of ventilation Min, 150~200r/min of rotating speed, fermentation full adjustment zymotic fluid pH value are 6.0~6.5, when fermentation is to 25~30h, with every The addition for rising 25~30mmol of zymotic fluid disposably adds Cys into zymotic fluid, continues fermentation 20-30h and obtains most Whole zymotic fluid；It is 18-26% that final zymotic fluid, which is concentrated under reduced pressure into solid content, is freeze-dried and produces yeast culture；

GSH final concentrations reach 3268-3308mg/L in the final zymotic fluid；

Viable bacteria content is in the yeast culture：7×10¹²-9×10¹²cfu/g；

The quality group of the seed culture medium turns into：(NH₄)₂SO₄6g/L, glucose 35g/L, K₂HPO₄·3H₂O 3g/ L, KH₂PO₄0.5g/L, dusty yeast 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH 6.0；

The quality group of the fermentation medium turns into：(NH₄)₂SO₄10g/L, molasses 150g/L, K₂HPO₄·3H₂O 8g/ L, KH₂PO₄0.5g/L, dusty yeast 5g/L, corn steep liquor 10g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH 6.0.

The saccharomyces cerevisiae is specially saccharomyces cerevisiae tlj2016, and it is micro- that the bacterial strain has been preserved in China on July 15th, 2016 Biological inoculum preservation administration committee common micro-organisms center, deposit number are CGMCC No.12789, preservation address：Beijing The institute 3 of Chaoyang District North Star West Road 1, Institute of Microorganism, Academia Sinica, postcode 100101；

The saccharomyces cerevisiae tlj2016 has following characteristic：1) 300g/L is reached to the tolerance of glucose, beneficial to it GSH is produced under the conditions of high concentration glucose；2) 3308mg/L is reached in 5L fermentation cylinder for fermentation production GSH final concentrations；3) it is resistance to By the ability extra-heavy of Cys, remain to slowly grow under the effect of 5mmol/L Cys, in 40mmol/L L- half Remain to keep GSH largely to synthesize under cystine effect；4) salt resistance ability reaches 18%, is advantageous to extend its application field.

Another object of the present invention is to provide the preparation method of above-mentioned catfish feed eating belt agent, comprises the following steps：According to Side, first successively by EM bacteria powders, yeast culture, Wheat Sprout Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, Glycine betaine, the 20-30% of calcium berry extract quality add V-type blending tank and uniformly mix 10-20min, speed of agitator 20-40r/ Min, then add remaining calcium berry extract and uniformly mix 20-40min, speed of agitator 40-60r/min, sterile filling, sealing, Pack and produce catfish feed eating belt agent.

Beneficial effect：

Catfish feed eating belt agent of the present invention is excellent with reference to its characteristic of ingesting based on catfish nutritional need and water body environment Change composition of raw materials, science compounding is greatly improved palatability and efficiency of feed utilization, has compared with strong anti-oxidation effect, eliminates free radical Composite flavor peptide；Cultures of S. cerevisiae with functional characteristics such as powerful trophism, bioactivity and inoxidizability；Can have Effect supplement calcium, iron and Soluble Fiber, the calcium berry extract that there is powerful embedding effect, extend the shelf life；It is remarkably improved anti- Freeze effect and stability, improve water resistance and glossiness, enhancing and promotion palatability, reduce the wormwood seed powder of the rate containing powder；It can carry For abundant nutrition material, bioactive substance, fragrance matter and the Wheat Sprout Powder in phytoenzyme source；Finally prepare a kind of palatability By force, the good catfish feed eating belt agent of production performance.1) feeding experiment shows：With same feeding and acclimation method, same day Catfish, same addition are fed when often management, same fish pond condition and same native catfish fingerling and injected volume In the case of, add that catfish feed palatability prepared by phagostimulant of the present invention is strong, and adult fish specification gap is small, and neatly, and then catfish is torn Sting, to compete the situation of looking for food slight, the low death rate is only 3%, and 75% is reduced than control group；Disease resistance is strong, and the incidence of disease is only 3%, 75% is reduced than control group；Digestibility is high, and tail increases weight by force, and 61.97% is improved than control group；Feed coefficient is low, and feedstuff-meat ratio is low, 39.47% is reduced than control group；Culture benefit is notable, output increased 65.51% in the case of same injected volume, greatly reduces foster Cost is grown, there is preferable practicality and advance.2) catfish feed prepares experiment and shown：Add catfish feed food calling of the present invention Catfish Moisture in Feed content and water activity prepared by agent reduces by 1.72% and 1.73% respectively, not easy infection miscellaneous bacteria, the shelf-life Long, stability is good；Rate containing powder reduces by 83.3%, and efficiency of feed utilization is high, and loss is small, is advantageous to maintain excellent aquatic environment；Powder Rate reduces by 80.88%, and feed caking property is high, and particle is neat, and glossiness is strong, is not easy in transport and storage process friction efflorescence, Raw material availability is improved, promotes the palatability of feed；Hardness improves 32.13%, and water resistance and durability degree are strong, are advantageous to Catfish is looked for food, and extension is looked for food the time, improves efficiency of feed utilization and digestibility；Gelatinization degree is high, improves 79.2%, is easy to catfish Digestion, absorb, considerably improve the digestive utilization ratio of catfish.Particular technique effect is shown in embodiment 7-11；Particular technique principle It is as follows：

1. saccharomyces cerevisiae tlj2016 culture viable bacteria contents of the present invention are up to 7 × 10¹²-9×10¹²Cfu/g, final fermentation GSH final concentrations reach 3268-3308mg/L in liquid, not only increase the protein level and enzymatic activity of catfish feed, Er Qieti The high bioactivity and inoxidizability of catfish feed, has greatly shown the functional characteristic of catfish feed eating belt agent, ferment of making wine Female tlj2016 except possess common yeast it is probiotic in addition to also there is following functions characteristic：1) tolerance of glucose is reached 300g/L, GSH is produced under the conditions of high concentration glucose beneficial to it；2) reach in 5L fermentation cylinder for fermentation production GSH final concentrations 3308mg/L；3) the ability extra-heavy of Cys is resistant to, remains to slowly grow under the effect of 5mmol/L Cys, Remain to keep GSH largely to synthesize under the effect of 40mmol/L Cys；4) salt resistance ability reaches 18%；Its science is compounded Can be further in catfish feed：(1) poisonous substances such as free radical, peroxide, heavy metal and aflatoxin are removed；(2) participate in The transhipment of amino acid (glutaminase, cysteine and other neutral amino acids)；(3) it is beneficial to the absorption of iron, the absorption of selenium, calcium Absorb, glutathione can also make the peroxidating aliphatic acid in feed revert to normal fat when absorbing or after absorbing；(4) Gastrointestinal tract mucosa epithelium is protected, is prevented because of the damage to intestinal mucosa such as inflammation, ischaemic, oxidation material；(5) store and provide It forms amino acid (especially cysteine)；(6) protein and DNA synthesis are participated in；(7) reducing substances is used as, beneficial to dimension Raw plain E, ascorbic reduction, maintain sulfydryl enzymatic activity.

2. wormwood seed powder prepared by the present invention is to be prepared using the seed wormwood seed of halophytes wormwood as raw material, contain Abundant dimethyl-β DMPTs (DMPT), are the good methyl donors of catfish, promote the Nutrition and Metabolism of catfish, are had excellent Food calling and growth promoting function, compounded with the raw material science such as composite flavor peptide, Wheat Sprout Powder, seawood meal, glycine betaine, tangerine peel powder, It is remarkably improved the palatability of catfish feed.Also, with stronger caking property while abundant nutrition material is provided, very Be adapted to prepare catfish pellet, be greatly improved pellet into graininess and integrality, the catfish feed water resistance of preparation Good, glossiness is strong, and rate containing powder is low, and efficiency of feed utilization is high；Wormwood cold resistance is strong simultaneously, and (wormwood seed) wormwood seed is rich in freeze proof egg In vain, handled through high-pressure electrostatic, high-pressure pulse electric auxiliary Induced by Salicylic Acid, low temperature are segmented Stress treatment and the organic knot of natural lighting Close so that under the stress and induction of outer boundary's environment, freeze proof matrix components obtain active seed containing freeze proof matrix itself Most comprehensively, most abundant synthesis and accumulation, improves the content of antifreeze protein, and then improve nutrition and biology in catfish feed The cryoprotective effects and stability of active material, extend the shelf-life of feed.

3. calcium berry extract prepared by the present invention is with calcic, iron and the higher calcium stem end of dietary fiber content, leaf of GAIGUO Be raw material with inulin, through ultrasonic cleaning, biology enzyme enzymolysis, microwave drying, extrusion, ultramicro grinding and be made；It is soluble Content of cellulose is high, bioactivity is strong, has important, positive role fiber to beneficial flora in catfish body, can particularly mend Fill calcium iron, extend the shelf-life of feed, compared with full diet fiber, its feature, bioactivity are more powerful；Simultaneously through ultra micro After crushing, add a certain amount of sodium acid carbonate, sealing and standing after adjusted moisture, can significantly increase dietary fiber puffed degree and The content of soluble dietary fiber, the feature of dietary fiber is improved, gained calcium berry extract thickening property is stronger, than single side Modified dietary fiber prepared by method improves 40-50%, and is not influenceed by acid, alkali, salt, significantly improves the water resistance of feed； Soluble Fiber cellulose content is high, improves 20-40% than modified dietary fiber prepared by single method, it is easier to by lactic acid bacteria profit With, growth and fertility of the raising lactic acid bacteria in animal body enteron aisle, the type and quantity of increase probiotics flora, reduction animal Body gut pH, improve animal body intestine microenvironment；Adsorption capacity is strong, after modified, the specific surface area increase of cellulose, Network is enriched, absorption affinity enhancing, chelating, absorption cholesterol and bile acids organic molecule ability it is stronger, suppress animal Absorption of the body to them；Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is stronger, effectively Prevent the weight of animals metal poisoning；Regulation and the resident time for maintaining gut flora, strengthen digestion and the absorbability of enteron aisle, Improve immunity of organisms；Gastrointestinal peristalsis is effectively facilitated, slows down and eliminates gasteremphraxis, the adverse reaction such as abdominal distension；Powerful embedding effect Influence of environment (oxygen, temperature, the illumination, water activity etc.) factor to product quality can be prevented, further stabilizes product Bioactivity and stability, extend the shelf-life of product.

4. composite flavor peptide prepared by the present invention is science compounding earthworm, sheep blood and soybean protein, through high-pressure pulse electric, The low temperature processing techniques such as complex enzyme subsection enzymolysis, ultrafiltration, segmentation reaction, freeze-drying and prepare have earthworm, sheep blood and big concurrently The composite flavor flavor peptide of beans flavor, taste compound enrich, and are particularly suitable for catfish taste, can not only improve the suitable of catfish feed Mouth property, but also the digestion and absorption of catfish feed nutrient can be promoted comprehensively, efficiency of feed utilization is improved, so as to subtract significantly The discharge rate of the nutriments such as organic matter, nitrogen phosphorus in few excreta, reduces pollution of the undigested material to water environment in feed. Whole process use low temperature processing techniques, avoid because high temperature and caused by albuminous degeneration and other bioactive substances and fragrance matter Loss, using high-voltage pulse electric field technology, it can not only prevent extraction process living contaminants, and significantly loose sheep blood and earthworm Structure between albumen and tissue, adds the permeability of tissue, is advantageous to the infiltration and contact of follow-up complex enzyme, improves enzymolysis The recovery rate of efficiency and flavor peptides, while also can directly increase the dissolution of soluble flavor peptides, pre-processed relative to existing high temperature It is more efficient, more environmentally friendly；Using subsection enzymolysis and Maillard reaction is segmented, further increases the yield of composite flavor peptide；Ion The science of solution and complex enzyme compounds the activity for further increasing complex enzyme, improves enzymolysis efficiency, reduces enzyme preparation Usage amount, the acquisition of composite flavor peptide is improved to greatest extent；Using freeze-drying, especially tool has been compounded to reaction solution science There is the sericin peptide taken of excellent cryoprotective effects, the oxidational losses and freezing for greatly reducing composite flavor peptide are lost, and improve compound wind The inoxidizability of gustin, a kind of palatability for being not only greatly improved catfish feed is finally made, improves efficiency of feed utilization, and With the composite flavor peptide for compared with strong anti-oxidation effect, eliminating free radical.

It effectively prevent 5. Wheat Sprout Powder prepared by the present invention carries out high-pressure pulse electric processing to the wheat of immersion process Soak pollution microbes, avoid generation stink and penetrate into Wheat Sprout Powder product, meanwhile, it can effectively strengthen cereal kind chrotoplast The relative permeability of wall and cell membrane, the water absorption rate for improving full cereal seed, promote seed to sprout in advance, improve superoxide anion certainly By generation speed and atriphos (ATP) content of base, the activation of a variety of biology enzymes of stratification wheat seed early stage and release Put, endosperm dissolving and functional nutrient composition, the synthesis of bioactive ingredients, antioxidant content, promote breathing generation of seed The effect of thanking, nutrition and functional materials, bioactive ingredients, the enrichment process of antioxidant content are accelerated, when shortening enrichment Between, nutrition and functional materials, bioactive ingredients, the content of antioxidant content are improved, soak with segmentation, rapidly heat up, Biological enzymolysis technique organically combines, and can further degrade kind of hide fiber element and a hemicellulose structure, increase kind skin permeability, activation Various bioactive substance (endogenous enzymes etc.) vigor are most strong, and enriching quantity is bigger, and Soluble Fiber cellulose content increases therewith, is wheat Catfish feed in bud powder provides trophic factors, better notable；By segmentation immersion, high-voltage pulse electric field technology and biology Enzymolysis organically combines, and shortens soak time, improves germination percentage and germination uniformity；Wheat Sprout Powder can be kept hot to greatest extent Quick property content of material, especially polyphenoils (glutathione, phospho phytate, vitamin C, polyphenol etc.), to greatest extent Ensure natural colored, the taste and flavor of Wheat Sprout Powder, while may also function as bactericidal action；Particularly moderately killed through high temperature, short time After embryo, in the case where not influenceing the bioactivity of wheat malt seed, with the absorption of moisture, wheat embryo is suppressed but not shadow Every enzyme and the growth of bioactive substance and the dissolving of endosperm are rung, bud growth is very short, and respiration is weak, and sprout damage is low, carries The high yield and quality of Wheat Sprout Powder, significantly improve the functional characteristic and digestion characteristics of phagostimulant.

6. the preparation method technique of catfish feed eating belt agent of the present invention is simple, low for equipment requirements, there will be powerful embedding The calcium berry extract of effect is added, mixed at twice, can be embedded other nutrition and bioactive substance wherein completely, maximum limit Reduce loss of the phagostimulant in catfish feed granulating, storage degree, improve the bioactivity and quality of phagostimulant Stability, effectively extend the shelf-life of phagostimulant.

It should be noted that catfish feed eating belt agent of the present invention has the technical effect that each component technical characteristic and method characteristic Mutually cooperate with, the result of interaction, the superposition of not simple technical characteristic (component function), each component technical characteristic has Machine with reference to and collaboration caused by effect considerably beyond the superposition of each monotechnics feature functionality and effect, have preferably advanced And practicality.

Embodiment

The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention It is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, it is not intended to limit the present invention Scope, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this The various changes carried out on the premise of invention spirit and scope to the material component in these embodiments and dosage or change Belong to protection scope of the present invention.

It is prepared by the raw material of embodiment 1

1. the preparation of yeast culture

The preparation method of the yeast culture, comprises the following steps：Take the saccharomyces cerevisiae tlj2016 after the activation of 1 ring oblique Face strain, it is seeded in the 500m L triangular flasks equipped with 100m L seed culture mediums and cultivates, in 30 DEG C, shaking speed 150r/min Under the conditions of cultivate 28h, obtain primary seed solution；Primary seed solution is pressed to 8% (v/v) inoculum concentration, is seeded to equipped with 100m L Carry out fermented and cultured in the 500m L triangular flasks of fermentation medium, fermentation time 28h, 30 DEG C, shaking speed 180r/min of temperature, Obtain secondary seed solution；Secondary seed solution is linked into the 5L fermentation tank constant temperature of dress 3L fermentation mediums with 8% (v/v) inoculum concentration Fermentation, 30 DEG C, ventilation 5L/min, rotating speed 200r/min of temperature, fermentation full adjustment zymotic fluid pH value is 6.0~6.5, works as hair When ferment is to 30h, Cys are disposably added into zymotic fluid with every liter of zymotic fluid 28mmol addition, continue to ferment 25h obtains final zymotic fluid；It is 22% that final zymotic fluid, which is concentrated under reduced pressure into solid content, is freeze-dried and produces Yeast Cultivation Thing；

GSH final concentrations reach 3308mg/L in the final zymotic fluid；

Viable bacteria content is in the yeast culture：9×10¹²cfu/g；

2. the preparation of composite flavor peptide

The preparation method of the composite flavor peptide, comprises the following steps：In mass ratio 5:3:2 weigh fresh earthworm, sheep blood And soybean protein, mix, rub to obtain compound, add the solion of 4 times of its quality, mix, regulation pH value is 7, in room temperature Under under the conditions of electric-field intensity 30kV/cm, burst length 400 μ s, pulse frequency 250Hz carry out high-pressure pulse electric processing 25min；Then the complex enzyme of mixture quality 0.06% is added, 40min is digested in 55 DEG C, is then cooled to 40 DEG C of enzymolysis 50min, 90 DEG C of enzyme deactivation 10min, are down to room temperature, and aseptic filtration obtains enzymolysis liquid, the ultrafiltration that enzymolysis liquid passes through 1000Da and 5000Da Membrane filtration, the permeate between two films is taken, concentrate must be digested for 20% by being concentrated under reduced pressure into solid content；Add enzymolysis concentration The reduced sugar of liquid quality 6%, successively after 40min and 140 DEG C of reaction 55min of 115 DEG C of reactions；By reaction solution by 1000Da and 5000Da ultrafiltration membrance filter, the permeate between two films is taken, concentrate must be reacted for 28% by being concentrated under reduced pressure into solid content； The sericin peptide taken of its quality 0.3% is added into reaction concentrate, is mixed, is freeze-dried and produces composite flavor peptide.

The solion is to contain sodium ion 20mg/L, zinc ion 18mg/L, potassium ion 13mg/L, calcium ion 10mg/L With the magnesium ion 8mg/L aqueous solution；

The complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase in mass ratio 5:3: 0.3:0.2 uniformly mixing；

The quality group of the reduced sugar turns into：Xylose:Ribose:Glucose:Fructose:Galactolipin=4:3:2:2:1.5.

3. the preparation of calcium berry extract

The preparation method of the calcium berry extract, comprises the following steps：By calcium stem end, leaf of GAIGUO and inulin in mass ratio 7: 5:2 uniformly mixing, are placed in supersonic wave cleaning machine and clean 4min in 200W, 30KHz, drain, be crushed to particle diameter 0.5mm, add The water that 1 times of fragment quality, regulation pH value are 6, add the biology enzyme of mixture quality 0.2%, and 12min is digested in 55 DEG C；Enzyme It is 50% that solution liquid, which is concentrated under reduced pressure into solid content, is put into microwave dryer in 2000W, 140 DEG C of progress intermittent dryings, is allowed to Moisture reaches 5%, is then crushed to particle diameter 0.5mm, adds the sodium acid carbonate of crushed material quality 0.3%, uniformly mixing, and adjustment is mixed Compound moisture is 13%, room temperature, sealing and standing 3h, in 150 DEG C of screw speed 110r/min, temperature condition extrusions, Then ultramicro grinding produces calcium berry extract to particle diameter 0.2mm；

The biology enzyme is cellulase, laccase, dextranase, seminase, pectase in mass ratio 3:2:2:1:0.7 Uniformly mixing.

4. the preparation of Wheat Sprout Powder

The preparation method of the Wheat Sprout Powder, comprises the following steps：Wheat is placed on the leaching that temperature is 15 DEG C, pH value is 7 30min is soaked in bubble liquid, soak contains the sodium acid carbonate and 0.3% compound enzyme that mass percent concentration is 0.2%, then 30 DEG C are warming up to 4 DEG C/min speed, continues to soak 4h, being finally warming up to 55 DEG C with 6 DEG C/min speed soaks 7min, whole Individual immersion process is divulged information once every 18min, ventilation pressure 0.14MPa, while in electric-field intensity 4kV/cm, the μ of burst length 200 S, pulse frequency 70Hz condition carry out high-pressure pulse electric processing, until wheat water content content is 42%；Wheat drip after immersion It is dry, carry out dark germination in growing floor, dark germination temperature is kept for 22 DEG C, and the dark germination time is 22h, the wheat drying after germination It is 6% to moisture, low-temperature grinding, crosses 80 mesh sieves and produce Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase, pentosanase in mass ratio 3:3:2:1.5 Even mixing.

5. the preparation of wormwood seed powder

The preparation method of the wormwood seed powder, comprises the following steps：By wormwood seed sabot, first in electric-field intensity 2kV/cm High-pressure electrostatic handles 6min；Then the soaking at room temperature 4h in the salicylic acid solution that concentration is 4mg/L, while in electric-field intensity 4kV/ High-pressure pulse electric processing is carried out under the conditions of cm, burst length 80 μ s, pulse frequency 120Hz；Rinse, drain, in 4 DEG C of standings 21h, 3d is then refrigerated at 2 DEG C successively, -4 DEG C of freezing 2d, -16 DEG C of freezing 12h, outdoor natural light is immediately placed at and shines after 3h immediately Crushed, freezed, add the sodium bicarbonate of freeze-dried powder quality 5%, uniformly mixing, crossed 80 mesh sieves and produce wormwood seed powder.

Yeast culture, composite flavor peptide, calcium berry extract, Wheat Sprout Powder, wormwood seed used in example 2 below -6 Powder is prepared by embodiment 1, and other raw materials are purchased in market.

Embodiment 2

100 parts of composite flavor peptide, 10 parts of calcium berry extract, 8 parts of Wheat Sprout Powder, 7 parts of glycine betaine, 5 parts of tangerine peel powder, wormwood seed 4 parts of powder, 4 parts of yeast culture, 3 parts of seawood meal, 1 part of EM bacteria powders；

Preparation method, comprise the following steps：According to formula, first successively by EM bacteria powders, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, the 25% of calcium berry extract quality add V-type blending tank Uniformly mixing 15min, speed of agitator 30r/min, then adds remaining calcium berry extract and uniformly mixes 30min, speed of agitator 50r/min, sterile filling, sealing, pack and produce catfish feed eating belt agent.

Embodiment 3

100 parts of composite flavor peptide, 8 parts of calcium berry extract, 6 parts of Wheat Sprout Powder, 5 parts of glycine betaine, 4 parts of tangerine peel powder, wormwood seed 2 parts of powder, 2 parts of yeast culture, 2 parts of seawood meal, 0.5 part of EM bacteria powders；

Preparation method, comprise the following steps：According to formula, first successively by EM bacteria powders, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, the 20% of calcium berry extract quality add V-type blending tank Uniformly mixing 10min, speed of agitator 20r/min, then adds remaining calcium berry extract and uniformly mixes 20min, speed of agitator 40r/min, sterile filling, sealing, pack and produce catfish feed eating belt agent.

Embodiment 4

100 parts of composite flavor peptide, 12 parts of calcium berry extract, 12 parts of Wheat Sprout Powder, 9 parts of glycine betaine, 6 parts of tangerine peel powder, wormwood 6 parts of seed powder, 6 parts of yeast culture, 4 parts of seawood meal, 1.5 parts of EM bacteria powders；

Preparation method, comprise the following steps：According to formula, first successively by EM bacteria powders, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, the 30% of calcium berry extract quality add V-type blending tank Uniformly mixing 20min, speed of agitator 40r/min, then adds remaining calcium berry extract and uniformly mixes 40min, speed of agitator 60r/min, sterile filling, sealing, pack and produce catfish feed eating belt agent.

Embodiment 5

100 parts of composite flavor peptide, 8 parts of calcium berry extract, 12 parts of Wheat Sprout Powder, 5 parts of glycine betaine, 6 parts of tangerine peel powder, wormwood seed 2 parts of powder, 6 parts of yeast culture, 2 parts of seawood meal, 1.5 parts of EM bacteria powders；

Preparation method, comprise the following steps：According to formula, first successively by EM bacteria powders, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, the 20% of calcium berry extract quality add V-type blending tank Uniformly mixing 20min, speed of agitator 20r/min, then adds remaining calcium berry extract and uniformly mixes 40min, speed of agitator 40r/min, sterile filling, sealing, pack and produce catfish feed eating belt agent.

Embodiment 6

100 parts of composite flavor peptide, 12 parts of calcium berry extract, 6 parts of Wheat Sprout Powder, 9 parts of glycine betaine, 4 parts of tangerine peel powder, wormwood seed 6 parts of powder, 2 parts of yeast culture, 4 parts of seawood meal, 0.5 part of EM bacteria powders；

Preparation method, comprise the following steps：According to formula, first successively by EM bacteria powders, yeast culture, wheat malt Powder, composite flavor peptide, seawood meal, wormwood seed powder, tangerine peel powder, glycine betaine, the 30% of calcium berry extract quality add V-type blending tank Uniformly mixing 10min, speed of agitator 40r/min, then adds remaining calcium berry extract and uniformly mixes 20min, speed of agitator 60r/min, sterile filling, sealing, pack and produce catfish feed eating belt agent.

The culture efficiency experiment of the catfish feed eating belt agent of the present invention of embodiment 7

1. fingerling and its source：It is purchased from the native catfish of certain aquaculture base；

2. test the selection in fish pond：It is respectively experimental group and control group to have selected two mouthfuls of fish ponds in certain production of catfish, fish pond Area is 3 mu, and the cultivation depth of water is 2.0-2.5 rice.The cultivation abundant water in fish pond is tested, water quality is pure and fresh pollution-free.Fish pond is set Independent culvert system, water inlet gate-valve chamber set three banister grooves, water jacket installation coarse strainer, inside groove installation conic net, rhone peace Fill anti-escape network.

3. the test period：2015.3.15-2015.11.15

4. cultural method：Test group and control group use following cultural method：

(1) pond preparation

1. pond is leveled：Including the dry pool, desilting and shine pond.

2. drug disinfection：7 days before fingerling stocking, change water with quick lime 100~130,000 gram/acre and splash sterilization of cleaning up the pond, it is desirable to Splash uniformly, pond Bu Liu dead angles.

3. pond water filling：Water filling on the 5th before fingerling stocking, fill level is 1.2 meters before fingerling stocking, after fingerling stocking Peak level is gradually filled to again.

(2) fingerling stocking：The experiment cultivation fingerling dispensing date is on March 15th, 2015, when selecting fingerling, it is desirable to fingerling body Mass color is sliding, without disability, and fingerling enters embathes sterilization 3~5 minutes before pond with 5% saline solution.It is thrown to test group and control at random Group fish pond, input situation are shown in Table 1.

The native Nian input situations of table 1

Fish pond	Kind	Specification (g/ tails)	Total mantissa	Average mu mantissa	Mu amount (Kg)	Total amount (Kg)
							Test group	Native catfish	50-100	9000	3000	225	675
Control group	Native catfish	50-100	9060	3020	226.5	679.5

(3) tame：Sit in the north facing the south in fishpond direction, with a distance from 2.5 meters from pond bank, 60 centimetres away from bottom of pond, taken with asbestos shingle If 3~5 food platforms.The same day that fingerling enters pond starts domestication and fed, and people sends sound before bait throwing in, makes fish Production conditions anti- Penetrate, when sound pauses, granular bait for fish is fed with wisp near bait table.2 times a day, every time 30~40 minutes, each bait throwing in Interval 10 seconds or so, so adheres to domestication 7~10 days, fingerling ingests granular bait for fish quantity from less to more, and the intensity of feeding is by weak To strong transformation, until taming successfully.

(4) raise：Test group is identical with control group fish pond inventory, adds this hair that mass ratio is 5% in feed respectively Catfish feed eating belt agent and commercially available phagostimulant prepared by bright embodiment 2, phagostimulant type, specification, date of manufacture all same.Daily Feed 2 times, with 9:00―10:00、15:00―16:00 respectively feeds once.Bait should be thrown on food platform after fish are tamed.Fish The feeding rate of class is 2%~6%, and daily ration of feeding should regard cultivation concrete condition and flexibly grasp, and be spent every time with eating up in 1 hour. " four is fixed " principle should be also followed when feeding.

(5) daily management

1. regulating and controlling water quality：The preceding pond water level of fingerling stocking filled new water after fingerling stocking at 1.2 meters or so every 5~7 days Once.Add 10~15 centimetres of water every time.The high temperature cultivation season water of 7-September adds to 2.0~2.5 meters, and former commonly using EM Dew improvement water quality, water transparency are 30~40 centimetres.

2. patrol the pool：It is daily patrol the pool and should follow early, middle and late patrol pool system.Check food situation, the variation of water of fish Deng pinpointing the problems and take measures in time.Particularly weather is sultry or has the situation of floating head omen to open aerator in time, keeps It is more than the mg/litre of water dissolved oxygen 5.

3. fish disease prevention and cure：Fish disease prevention and cure according to " put prevention first, prevent and treat combination " policy.Accomplish it is disease-free first prevent, cultivating Chlorine dioxide or lime disinfection water body is commonly used in period.

5. pilot project and result：

Observed in the whole test period, detect catfish incidence, death condition and note down, statistical experiment group and control The group fish pond incidence of disease and the death rate；Statistical experiment group and control group yield when going out the pool (mantissa, cabrage, gross weight, tail increase weight)； As a result such as table 2：

Table 2：Native catfish feeding experiment result

Above result of the test shows：With same feeding and acclimation method, same daily management, same fish pond condition With feed catfish when same native catfish fingerling and injected volume, in the case of same addition, add food calling of the present invention Catfish feed palatability prepared by agent is strong, and adult fish specification gap is small, and neatly, and then catfish baits, to compete the situation of looking for food slight, extremely It is only 3% that it is low, which to die rate, and 75% is reduced than control group；Disease resistance is strong, and the incidence of disease is only 3%, and 75% is reduced than control group；Digestibility Height, tail are increased weight by force, and 61.97% is improved than control group；Feed coefficient is low, and feedstuff-meat ratio is low, and 39.47% is reduced than control group；Support Grow remarkable benefit, output increased 65.51% in the case of same injected volume, greatly reduce aquaculture cost, have preferably practical Property and advance.

It should be noted that：Catfish feed eating belt agent prepared by 3-6 of the embodiment of the present invention equally has above-mentioned experiment effect, It is between each embodiment and little with above-mentioned experiment effect otherness.

Embodiment 8 adds catfish feed primary quality measure detection prepared by phagostimulant of the present invention

Catfish feed eating belt agent and commercially available same size, type and date of manufacture prepared by the embodiment of the present invention 2 is taken to lure Food agent is added in catfish feed with the addition of mass ratio 5% uniformly to be mixed, pelletizes, drying, respectively as test group and control Group, according to national aquatic granulated feed relevant criterion, detection moisture, water activity, pulverization rate, rate containing powder, hardness, gelatinization degree etc. Leading indicator, testing result such as table 3：

Table 3：Catfish feed primary quality measure testing result

Project	Moisture (%)	Water activity	Pulverization rate (%)	Rate containing powder (%)	Hardness (g)	Gelatinization degree (%)
							Test group	5.7	0.283	1.3	0.3	8360.77	86
Control group	5.8	0.288	6.8	1.8	6327.65	48
							Difference	- 1.72%	- 1.73%	- 80.88%	- 83.3%	32.13%	79.2%

Result above shows：Compared with commercially available, add catfish Moisture in Feed prepared by catfish feed eating belt agent of the present invention and contain Amount and water activity reduce by 1.72% and 1.73% respectively, and easy infection miscellaneous bacteria, long shelf-life, stability is not good；Rate containing powder reduces 83.3%, efficiency of feed utilization is high, and loss is small, is advantageous to maintain excellent aquatic environment；Pulverization rate reduces by 80.88%, and feed glues Knot property is high, and particle is neat, and glossiness is strong, is not easy, in transport and storage process friction efflorescence, to improve raw material availability, promote The palatability of feed；Hardness improves 32.13%, and water resistance and durability degree are strong, is advantageous to catfish and looks for food, and extension is looked for food the time, Improve efficiency of feed utilization and digestibility；Gelatinization degree is high, improves 79.2%, is easy to catfish digestion, absorbs, considerably improves The digestive utilization ratio of catfish.

Saccharomyces cerevisiae tlj2016 fermentations production GSH capacity experimentals under the conditions of the high sugar of embodiment 9

(1) Shaking culture

The ring of tlj2016 slant strains one is taken, accesses and 150rpm in the 250mL shaking flasks of 30mL Shake flask mediums is housed, 30 DEG C Culture 30h obtains seed liquor；

The Shake flask medium quality group turns into：(NH4)₂SO₄6g/L, glucose 35g/L, K₂HPO4·3H₂O 3g/L, KH₂PO₄0.5g/L, dusty yeast 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO₄0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH 6.0；

(2) 5L fermentation tank cultures

Seed liquor is pressed into 10% inoculum concentration, access is equipped with the fermentation tank of 3L fermentation mediums, 30 DEG C, throughput 6L/ Min, tank press 0.03MPa, 500rpm, carry out fermented and cultured under the conditions of permanent pH6.0, when fermenting to 30h, disposably add final concentration For 25mmol/L Cys, total fermentation time 50h；

The fermentation medium quality group turns into：(NH₄)₂SO₄10g/L, glucose 100g/L, K₂HPO₄·3H₂O 8g/ L, KH₂PO₄0.5g/L, dusty yeast 11g/L, MnSO₄0.1g/L, KCL 0.1g/L, FeSO4 0.1g/L, MgSO₄·7H₂O 0.1g/L, surplus are water, pH6.0；

After fermentation ends, the content for determining GSH in zymotic fluid is 3308mg/L.

The saccharomyces cerevisiae tlj2016L- cysteines tolerance of embodiment 10 is tested

By starting strain and each ring of tlj2016 slant strains, the 250mL equipped with 30mL Shake flask mediums is respectively connected to 150rpm in shaking flask, 30 DEG C are cultivated, and when culture is to 12h, the Guang ammonia of L- half of different final concentrations is added into shaking flask Acid, 10h is further cultured for, determines dry cell weight, as a result table 4,5；

Shake flask medium (g/L)：(NH₄)₂SO₄6th, glucose 20, K₂HPO₄·3H₂O 3、KH₂PO₄0.5th, dusty yeast 11, MnSO₄ 0.1、KCL 0.1、FeSO4 0.1、MgSO₄·7H₂O 0.1, pH6.0；

Table 4：Starting strain Cys tolerance

Cys concentration mmol/L	0	5	10	15	20	40
							Starting strain dry weight g/L	22.6	15.7	10.2	4.3	2.2	0.8
GSH concentration (mg/L)	35.6	46.7	43.2	40.7	37.9	25.3

The tlj2016L- cysteine tolerances of table 5

Cys concentration mmol/L	0	5	10	15	20	40
							Tlj2016 dry weights g/L	25.7	28.5	23.6	21.2	20.6	18.7
GSH concentration (mg/L)	73.2	98.3	113.5	121.7	127.5	135.8

It is can be seen that from the result of table 4,5 for starting strain, Cys added in culture medium, cell stops life It is long, and start self-dissolving, cause GSH growth rates to be reduced with the rise of Cys concentration；And the Guang ammonia of low concentration L- half Under acid, tlj2016 still is able to slowly grow, and with the raising of Cys concentration, the dry cell weight of tlj2016 bacterial strains is slow Decline, and GSH concentration sustainable growths, this result are beneficial in GSH production processes by adding the Guangs of precusor amino acids-L- half Propylhomoserin proposes the production for promoting GSH.

The saccharomyces cerevisiae tlj2016 salt resistance abilities of embodiment 11 are tested

Take tlj2016 bacterium solutions 1mL inoculation strain in containing different NaCl concentrations (concentration gradients 0%, 2%, 5%, 10%th, 15%, 10mL YPD fluid nutrient mediums (pH=6.5) 18%), are placed at 30 DEG C and cultivate 24h respectively, each handle 3 Individual repetition.Respectively take 1ml samples bacterium solution to be mixed in 9ml physiological saline, prepare dilution factor solution, take 0.1ml dilutions to consolidate in YPD Be coated with body flat board, be inverted in 30 DEG C of biochemical cultivation cases culture 36 hours (each dilution factor do 3 parallel) record calculate it is flat Bacterium number number on plate.It the results are shown in Table 6, it is known that the resistance to salinity of the bacterium is 18%, illustrates that saccharomyces cerevisiae tlj2016 not only can be with Survived in conventional environment, still there is vigor under high salt conditions, can be applied to the high salt food processing such as soy sauce, curing food Sugar production glutathione is consumed in journey.

The salt resistance ability of table 6 detection (× 10⁷cfu/ml)

Claims

1. a kind of catfish feed eating belt agent, mainly prepared by the raw material of following parts by weight：100 parts of composite flavor peptide, calcium fruit carries Take thing 8-12 parts, Wheat Sprout Powder 6-12 parts, glycine betaine 5-9 parts, tangerine peel powder 4-6 parts, wormwood seed powder 2-6 parts, yeast culture 2-6 Part, seawood meal 2-4 parts, EM bacteria powder 0.5-1.5 parts.

2. catfish feed eating belt agent as claimed in claim 1, it is characterised in that the preparation method of the composite flavor peptide, bag Include following steps：4-6 in mass ratio:2-4:1-3 weighs fresh earthworm, sheep blood and soybean protein, mixes, rubs to obtain compound, The solion of 2-5 times of its quality is added, is mixed, regulation pH value is 6-8, at room temperature in electric-field intensity 25-35kV/cm, arteries and veins Time 300-500 μ s are rushed, high-pressure pulse electric processing 20-30min is carried out under the conditions of pulse frequency 200-300Hz；Then add Mixture quality 0.04-0.08% complex enzyme, 30-50min is digested in 50-60 DEG C, be then cooled to 35-45 DEG C of enzymolysis 40- 60min, 90 DEG C of enzyme deactivation 10min, are down to room temperature, and aseptic filtration obtains enzymolysis liquid, the ultrafiltration that enzymolysis liquid passes through 1000Da and 5000Da Membrane filtration, the permeate between two films is taken, concentrate must be digested for 18-22% by being concentrated under reduced pressure into solid content；Add enzymolysis Concentrate quality 5-7% reduced sugar, successively after 112-118 DEG C is reacted 30-50min and 135-145 DEG C of reaction 45-65min； Ultrafiltration membrance filter by reaction solution by 1000Da and 5000Da, takes the permeate between two films, is concentrated under reduced pressure into solid content and contains Concentrate must be reacted for 26-30% by measuring；Its quality 0.2-0.4% sericin peptide taken is added into reaction concentrate, is mixed, freezing is dry It is dry to produce composite flavor peptide；

The complex enzyme is earthworm serine protease, alkali protease, flavor protease, lipase 4-6 in mass ratio:2-4: 0.2-0.4:0.1-0.3 is uniformly mixed.

3. catfish feed eating belt agent as claimed in claim 2, it is characterised in that the solion is to contain sodium ion 17- 22mg/L, zinc ion 15-20mg/L, potassium ion 12-14mg/L, calcium ion 9-11mg/L and magnesium ion 6-10mg/L it is water-soluble Liquid.

4. catfish feed eating belt agent as claimed in claim 2, it is characterised in that the quality group of the reduced sugar turns into：Xylose: Ribose:Glucose:Fructose:Galactolipin=3-5:2-4:1-3:1-3:1-2.

5. catfish feed eating belt agent as claimed in claim 1, it is characterised in that the preparation method of the calcium berry extract, bag Include following steps：By calcium stem end, leaf of GAIGUO and inulin 5-9 in mass ratio:3-6:1-3 is uniformly mixed, and is placed in supersonic wave cleaning machine In in 200W, 30KHz clean 3-5min, drain, be crushed to particle diameter 0.3-0.7mm, add 0.5-1.5 times of fragment quality Water, regulation pH value are 5-7, add mixture quality 0.1-0.3% biology enzyme, and 10-15min is digested in 50-60 DEG C；Enzymolysis liquid It is 40-60% to be concentrated under reduced pressure into solid content, is put into microwave dryer and carries out intermittent drying in 2000W, 130-150 DEG C, It is allowed to moisture and reaches 4-6%, be then crushed to particle diameter 0.4-0.6mm, adds crushed material quality 0.2-0.4% sodium acid carbonate, Uniformly mixing, adjustment mixture moisture is 12-14%, room temperature, sealing and standing 2-4h, in screw speed 105-115r/ 140-160 DEG C of min, temperature condition extrusion, then ultramicro grinding to particle diameter 0.1-0.3mm produce calcium berry extract；

The biology enzyme is cellulase, laccase, dextranase, seminase, pectase 2-4 in mass ratio:1-3:1-3: 0.5-1.5:0.4-1 is uniformly mixed.

6. catfish feed eating belt agent as claimed in claim 1, it is characterised in that the preparation method of the Wheat Sprout Powder, including Following steps：Wheat is placed in the soak that temperature is 10-18 DEG C, pH value is 6-8 and soaks 20-40min, soak contains matter Percent concentration is measured as 0.1-0.3% sodium acid carbonate and 0.2-0.4% compound enzyme, then with 3-5 DEG C/min speed liter Temperature continues to soak 3-5h to 28-32 DEG C, is finally warming up to 50-60 DEG C of immersion 5-8min, whole leaching with 5-7 DEG C/min speed Bubble process is divulged information once every 15-20min, ventilation pressure 0.13-0.15MPa, while when electric-field intensity 3-5kV/cm, pulse Between 100-300 μ s, pulse frequency 60-80Hz condition carry out high-pressure pulse electric processing, until wheat water content content is 40- 45%；Wheat after immersion is drained, and carries out dark germination in growing floor, and dark germination temperature is kept for 20-24 DEG C, and the dark germination time is 20-24h, wheat drying to the moisture after germination are 5-8%, low-temperature grinding, cross 60-80 mesh sieves and produce Wheat Sprout Powder；

The compound enzyme is cellulase, 1,4 beta-glucanase, zytase, pentosanase 2-4 in mass ratio:2-4:1-3:1-2 Uniformly mixing.

7. catfish feed eating belt agent as claimed in claim 1, it is characterised in that the preparation method of the wormwood seed powder, including Following steps：By wormwood seed sabot, 5-7min is handled in electric-field intensity 1-3kV/cm high-pressure electrostatics first；Then it is 3- in concentration Soaking at room temperature 2-5h in 5mg/L salicylic acid solution, while in electric-field intensity 2-6kV/cm, burst length 50-100 μ s, pulse High-pressure pulse electric processing is carried out under the conditions of frequency 100-150Hz；Rinse, drain, stand 18-24h in 3-5 DEG C, then successively 2-4d is refrigerated at 1-3 DEG C, -3--5 DEG C of freezing 1-3d, -15--18 DEG C of freezing 10-15h, outdoor natural light is immediately placed at and shines 2-4h Crushed, freezed immediately afterwards, add freeze-dried powder quality 3-8% sodium bicarbonate, uniformly mixing, crossed 80-100 mesh sieves and produce wormwood Seed powder.

8. catfish feed eating belt agent as claimed in claim 1, it is characterised in that the preparation method of the yeast culture, bag Include following steps：The saccharomyces cerevisiae tlj2016 slant strains after the activation of 1 ring are taken, are seeded to equipped with 100mL seed culture mediums Cultivated in 500mL triangular flasks, 25~30h is cultivated under the conditions of 28~30 DEG C, 150~200r/min of shaking speed, obtains one-level Seed liquor；Primary seed solution is pressed to 5~10% (v/v) inoculum concentration, is seeded to the 500mL tri- equipped with 100mL fermentation mediums Fermented and cultured is carried out in the bottle of angle, 25~30h of fermentation time, 28~30 DEG C of temperature, 150~200r/min of shaking speed, obtains two Level seed liquor；The 5L fermentation tanks constant temperature that secondary seed solution is linked into dress 3L fermentation mediums with 5~10% (v/v) inoculum concentrations is sent out Ferment, 28~30 DEG C, 4~6L/min of ventilation, 150~200r/min of rotating speed of temperature, fermentation full adjustment zymotic fluid pH value are 6.0 ~6.5, when fermentation is to 25~30h, L- is disposably added into zymotic fluid with every liter of 25~30mmol of zymotic fluid addition Cysteine, continue fermentation 20-30h and obtain final zymotic fluid；It is 18- that final zymotic fluid, which is concentrated under reduced pressure into solid content, 26%, it is freeze-dried and produces yeast culture；

The saccharomyces cerevisiae tlj2016 deposit numbers are CGMCC No.12789.

9. catfish feed eating belt agent as claimed in claim 8, it is characterised in that GSH final concentrations reach in the final zymotic fluid To 3268-3308mg/L；Viable bacteria content is in the yeast culture：7×10¹²-9×10¹²cfu/g。

10. the preparation method of catfish feed eating belt agent as described in claim 1-9 is any, it is characterised in that comprise the following steps： According to formula, first successively by EM bacteria powders, yeast culture, Wheat Sprout Powder, composite flavor peptide, seawood meal, wormwood seed powder, old Pi Fen, glycine betaine, the 20-30% of calcium berry extract quality add V-type blending tank and uniformly mix 10-20min, speed of agitator 20- 40r/min, then add remaining calcium berry extract and uniformly mix 20-40min, speed of agitator 40-60r/min is sterile filling, close Seal, pack and produce catfish feed eating belt agent.