CN107488078A - A kind of organic fertilizer and preparation method with insect-expelling function based on biological bacteria - Google Patents
A kind of organic fertilizer and preparation method with insect-expelling function based on biological bacteria Download PDFInfo
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- CN107488078A CN107488078A CN201710679181.9A CN201710679181A CN107488078A CN 107488078 A CN107488078 A CN 107488078A CN 201710679181 A CN201710679181 A CN 201710679181A CN 107488078 A CN107488078 A CN 107488078A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of organic fertilizer and preparation method with insect-expelling function based on biological bacteria, it is related to crop technical field.The organic fertilizer with insect-expelling function based on biological bacteria includes the mixture that is formed by biological bacteria and substrate fermentation, and biological expelling parasite medicament, wherein, matrix includes string and microbial nutrition composition, biological bacteria, and the weight ratio of biological expelling parasite medicament and matrix is：(1~5)：(10~20)：(20~50).The present invention is used to make agricultural organic fertilizer while have biological insect-expelling function, can promote agricultural development, but not grain-production and environment are negatively affected.
Description
Technical field
The present invention relates to biological technical field, is related to a kind of organic fertilizer with insect-expelling function based on biological bacteria and preparation
Method.
Background technology
The insect pest of crops, disease are that the generation of pest and disease damage can make crops tight an important factor for influenceing its yield all the time
The underproduction again, or even total crop failure, therefore, the effectively control to pest and disease damage is extremely important.China's agricultural is with chemical pesticide, change for a long time
Pest and disease damage is prevented and treated based on bactericide, in the past 20 years, the administration of chemical pesticide plays for the safety in production of guarantee China agricultural
Important effect, but some negative effects are thus also brought, for example, being too dependent on chemical fertilizer and agricultural chemicals for a long time, cause
The waste of a large amount of non-renewable resources, farmland soil qualitative change is bad, and fertility declines, crop products poisoning residual, quality reduce and
The problem of food and environmental pollution, becomes increasingly conspicuous.
The content of the invention
It is an object of the invention to provide a kind of organic fertilizer and preparation method with insect-expelling function based on biological bacteria, uses
In making organic fertilizer while there is insect-expelling function, agricultural development can be promoted, but not negative shadow is brought to grain-production and environment
Ring.
To reach above-mentioned purpose, first aspect present invention provides a kind of organic fertilizer with insect-expelling function based on biological bacteria
Material, is adopted the following technical scheme that：
The organic fertilizer with insect-expelling function based on biological bacteria includes the mixture formed by biological bacteria and substrate fermentation, with
And biological expelling parasite medicament, wherein, matrix includes string and microbial nutrition composition, biological bacteria, biological expelling parasite medicament and base
The weight ratio of matter is：(1~5)：(10~20)：(20~50).
Alternatively, biological expelling parasite medicament is included through one in the treated tobacco of biological bacteria, sweet wormwood, fennel, garlic, pepper
Kind is a variety of.
Alternatively, biological bacteria is the one or more in bacillus subtilis, bacillus megaterium, bacillus licheniformis.
Alternatively, the string in matrix includes the one or more in wood chip, rice husk, rice chaff, stalk.
Alternatively, the microbial nutrition composition in matrix include animal wastes, animal-based protein, plant source protein,
One or more in single cell protein.
Because the organic fertilizer with insect-expelling function provided by the invention based on biological bacteria includes being sent out by biological bacteria and matrix
The mixture that ferment is formed, and biological expelling parasite medicament, wherein, matrix includes string and microbial nutrition composition, biological bacteria,
Biological expelling parasite medicament and the weight ratio of matrix are：(1~5)：(10~20)：(20~50), so that the organic fertilizer is promoting
While entering agricultural development, it will not be negatively affected.
Second aspect of the present invention provides a kind of preparation method of the organic fertilizer with insect-expelling function based on biological bacteria, uses
Following technical scheme：
The preparation method of the organic fertilizer with insect-expelling function based on biological bacteria includes：
Step S1, biological bacteria, biological expelling parasite medicament and matrix are weighed, wherein, biological bacteria, biological expelling parasite medicament and matrix
Weight ratio is：(1~5)：(10~20)：(20~50)；
Step S2, the biological bacteria weighed and matrix are mixed, and fermented；
Step S3, in the mixture for forming biological expelling parasite medicament added to fermentation, the organic fertilizer based on biological bacteria is obtained
Material.
Alternatively, the biological bacteria in step S1 cultivates to be formed in the following way：
Raw material is weighed and mixed, and raw material includes the one or more in corn, soya bean, potato, glutinous millet；
Mixed raw material is handled；
The strain of biological bacteria is implanted into raw material after treatment, and is cultivated；
After the completion of cultivation, biological bacteria is extracted.
Alternatively, the biological expelling parasite medicament in step S1 obtains in the following way：
Tobacco is soaked with the liquid comprising biological bacteria, and is crushed, and/or, sweet wormwood is soaked with the liquid comprising biological bacteria, and
It is broken, and/or, fennel is soaked with the liquid comprising biological bacteria, and crush, and/or, soaked with the liquid comprising biological bacteria big
Garlic, and crush, and/or, pepper is soaked with the liquid comprising biological bacteria, and crush.
Alternatively, preparation method is additionally included between step S1 and step S2, is carried out high-temperature process to matrix, is killed matrix
In bacterium after, matrix is cooled to the fermentation temperature needed for step S2.
Alternatively, preparation method is additionally included in after step S3, and granulation processing is carried out to obtained organic fertilizer.
The preparation method of the organic fertilizer with insect-expelling function based on biological bacteria in the present invention has and as described above
The organic fertilizer identical beneficial effect with insect-expelling function based on biological bacteria, herein without limiting.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, embodiment will be described below
In the required accompanying drawing used be briefly described, it should be apparent that, drawings in the following description be only the present invention some
Embodiment, for those of ordinary skill in the art, on the premise of not paying creative work, can also be attached according to these
Figure obtains other accompanying drawings.
Fig. 1 is the flow of the preparation method of the organic fertilizer with insect-expelling function based on biological bacteria in the embodiment of the present invention
Figure；
The flow chart of the breeding method of biological bacteria in Fig. 2 embodiment of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is part of the embodiment of the present invention, rather than whole embodiments.Based on this hair
Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, belongs to the scope of protection of the invention.
Embodiment one
The embodiment of the present invention provides a kind of organic fertilizer with insect-expelling function based on biological bacteria, specifically, should be based on life
The organic fertilizer with insect-expelling function of thing bacterium includes the mixture formed by biological bacteria and substrate fermentation, and biological anthelmintic
Agent, wherein, matrix includes string and microbial nutrition composition, biological bacteria, and the weight ratio of biological expelling parasite medicament and matrix is：
(1~5)：(10~20)：(20~50).
In above-mentioned each composition, the effect of matrix is to adhere to biological bacteria, is uniformly dispersed, and is provided for the breeding of biological bacteria
Nutriment, and as the fertilizer needed for crop growth.Preferably, matrix passes through sterilization treatment, such as high-temperature sterilization processing,
And then it can effectively kill the effect that the organic fertilizer in the application is improved except the harmful bacteria in matrix.Plant is fine in preferred substrate
Weight ratio between dimension and microbial nutrition composition is (0.1~10):1, optimal is 1:1.Alternatively, the string in matrix
Including the one or more in wood chip, rice husk, rice chaff, stalk；Microbial nutrition composition in matrix includes animal wastes, animal
One or more in endogenous binding protein, plant source protein, single cell protein.Wherein, inventor has found, single cell protein helps
In the quantity in stock of raising biological bacteria, therefore contain single cell protein in the embodiment of the present invention in preferred substrate.Most preferably matrix is
Rice husk, cow dung, fish meal, the mixture of soy meal and single cell protein.
The effect of biological expelling parasite medicament is to walk quickly and keep away insect for crops.Alternatively, biological expelling parasite medicament is included through biology
One or more in the treated tobacco of bacterium, sweet wormwood, fennel, garlic, pepper, above-mentioned each composition is common plant, is entered
And can be while effectively insect is walked quickly and kept away, growth that will not be to crops produces harmful effect, will not also cause harmful substance
Residual.It should be noted that the mode of biological bacteria processing is to be soaked with the liquid comprising biological bacteria, liquid can be water, herein
It can be same or not with the biological bacteria in organic fertilizer to soak tobacco, sweet wormwood, fennel, garlic, the biological bacteria of pepper etc.
Of the same race, herein without limiting, those skilled in the art can be selected according to being actually needed.
The effect of biological bacteria is according to the different and different of its species.Alternatively, biological bacteria is bacillus subtilis, huge bud
One or more in spore bacillus, bacillus licheniformis.The species of certain biological bacteria can also be other, such as：Su Yun gold buds
Spore bacillus, bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, mycorrhizal fungi, azotobacter vinelandii, photosynthetic bacteria group, condensation
One or more in bacillus, aspergillus oryzae, Paecilomyces lilacinus.
The effect of each biological bacteria is as follows above：Bacillus subtilis：Increase crop anti-adversity, fixed nitrogen；Bacillus megaterium：
Discharge micro- in solvable phosphorus potassium element and calcium, sulphur, magnesium, iron, zinc, molybdenum, manganese etc.；Bacillus licheniformis：Disease-resistant, killing has
Evil bacterium；Bacillus thuringiensis：Desinsection, there is specific cytotoxicity to arthropods such as Lepidopteras；Bacillus laterosporus：Promote
Root, sterilization and degraded heavy metal；Colloid bacillus cereus：There are Soluble phosphorus, Potassium release and fixed nitrogen function, secrete a variety of enzymes, strengthen crop pair
The resistance of some diseases；Streptomyces jingyangensis：With the ability for strengthening soil fertility, stimulating plant growth；Mycorrhizal fungi：Expand
Root Absorption face, increase the absorbability to the element (particularly phosphorus) outside primitive root hair absorption region；Azotobacter vinelandii：It is fixed empty
Nomadic nitrogen in gas, volume increase；Photosynthetic bacteria group：It is the main force of rich soil and promotion animal and plant growth；Bacillus coagulans：
The pernicious gases such as ammonia in environment, hydrogen sulfide can be reduced, improve the content of amino acid in fruit；Aspergillus oryzae：Make in stalk
Organic matter turns into the nutrition needed for plant growth, improves the soil organism, improves soil texture；Paecilomyces lilacinus：To a variety of lines
Worm has preventing and treating efficiency, is the preventing and treating most promising biological prevention and control agent of root-knot nematode.
In addition, the use of biological germ fertilizer of the present invention can promote plant growth healthy and strong, the immunity of crop is significantly greatly increased,
Effectively defence crop disease.
Organic fertilizer in the embodiment of the present invention can also include sugar residue and plant ash, wherein, sugar residue can be beet sugar
Any of slag, corn sugar residue, sugarcane sugar residue, watermelon sugar residue, sweet sorghum slag or the mixture more than any two.Sugar residue can
Nutrition is provided for biological bacteria and crops, plant ash can also play a part of expelling parasite.
Because the organic fertilizer with insect-expelling function provided by the invention based on biological bacteria includes being sent out by biological bacteria and matrix
The mixture that ferment is formed, and biological expelling parasite medicament, wherein, matrix includes string and microbial nutrition composition, biological bacteria,
Biological expelling parasite medicament and the weight ratio of matrix are：(1~5)：(10~20)：(20~50), so that the organic fertilizer is promoting
While entering agricultural development, it will not be negatively affected.
Embodiment two
The embodiment of the present invention provides a kind of preparation method of the organic fertilizer with insect-expelling function based on biological bacteria, the preparation
Method is to prepare the organic fertilizer with insect-expelling function based on biological bacteria described in embodiment one, specifically, such as Fig. 1 institutes
Show, being somebody's turn to do the preparation method of the organic fertilizer with insect-expelling function based on biological bacteria includes：
Step S1, biological bacteria, biological expelling parasite medicament and matrix are weighed, wherein, biological bacteria, biological expelling parasite medicament and matrix
Weight ratio is：(1~5)：(10~20)：(20~50).
Wherein, the biological bacteria in step S1 be able to can also be made by oneself by buying acquisition, this hair in subsequent embodiment three
Bright embodiment carries out citing description to the method for voluntarily cultivating biological bacteria.Alternatively, the biological expelling parasite medicament in step S1 passes through
Following manner obtains：Tobacco is soaked with the liquid comprising biological bacteria, and is crushed, soaks sweet wormwood with the liquid comprising biological bacteria, and
It is broken, and/or, fennel is soaked with the liquid comprising biological bacteria, and crush, and/or, soaked with the liquid comprising biological bacteria big
Garlic, and crush, and/or, pepper is soaked with the liquid comprising biological bacteria, and crush.
Step S2, the biological bacteria weighed and matrix are mixed, and fermented.
Exemplarily, fermentation process can include：Obtained mixture is warming up to 60~70 DEG C, is preferably warming up to 65
DEG C, fermented；The tunning insulation for making to obtain is fermented 5~18 days at 60~70 DEG C, is preferably fermented 7~15 days, more preferably
Fermentation 10 days；The tunning insulation for making to obtain is fermented 7~20 days at 45~55 DEG C, is preferably incubated at 50 ± 2 DEG C, is more preferably protected
Temperature is at 50 DEG C.During the fermentation, can often be stirred, for example, can every two days or threeway turn over once.
It is as follows the reasons why each temperature preferred value during the fermentation：Because during the fermentation, fermentation condition can not be accomplished
Food or the clean level of industrial goods fermentation, are easily polluted by parasite and miscellaneous bacteria, it is therefore desirable to using caused by fermentation
High temperature is killed and suppressed, and still, inventor has found that the high temperature higher than 70 DEG C can influence the life of most of biological bacterias in the present invention
Long, breeding and fermentation, or even some biological bacterias can be thoroughly killed, fermentation is had adverse effect on, so that organic fertilizer
Quality is greatly reduced.Therefore, temperature should be controlled below 70 DEG C in the preparation method of organic fertilizer of the invention, and should be protected
Hold appropriate high temperature (50 DEG C or so).
Preparation method in the embodiment of the present invention is additionally included between step S1 and step S2, and high-temperature process is carried out to matrix
(for example, treatment temperature is set in 95~100 DEG C, processing time is 1~2 hour), and after killing the bacterium in matrix, by matrix
It is cooled to the fermentation temperature needed for step S2.Wherein, the miscellaneous bacteria in matrix can be effectively killed to matrix progress high-temperature process,
Matrix is cooled to the fermentation temperature needed for step S2 can prevent will be follow-up mixed because substrate temperature is too high
Biological bacteria is also killed.
Step S3, in the mixture for forming biological expelling parasite medicament added to fermentation, the organic fertilizer based on biological bacteria is obtained
Material.
Should be after the mixture cooling that fermentation is formed, such as room temperature is cooled to, biological expelling parasite medicament is added to fermentation shape
Into mixture in, and being sufficiently stirred makes it well mixed.Further, it is also possible to add other things in the mixture that fermentation is formed
Matter, such as sugar residue and plant ash.
In addition, in order to improve the convenience used of the organic fertilizer in the present invention, the preparation side in the embodiment of the present invention
Method is additionally may included in after step S3, and granulation processing is carried out to obtained organic fertilizer.Granulation processing can select special
Comminutor is carried out, and is not be described in detail herein., can also be in outermost layer Paris white for being granulated the organic fertilizer completed
Face powder, makes particle surface more smooth, prevents from luming, and the organic fertilizer after granulation is entered into dryer low temperature drying, through cold
But machine cools down.
Because the organic fertilizer with insect-expelling function based on biological bacteria prepared using above-mentioned preparation method is included by biology
The mixture that bacterium and substrate fermentation are formed, and biological expelling parasite medicament, wherein, matrix include string and microbial nutrition into
Divide, biological bacteria, the weight ratio of biological expelling parasite medicament and matrix is：(1~5)：(10~20)：(20~50), so that this has
Machine fertilizer will not be negatively affected while agricultural development is promoted.
Embodiment three
The breeding method of biological bacteria is described in detail the embodiment of the present invention.Specifically, as shown in Fig. 2 the biology
The breeding method of bacterium includes：
Step S1 ', raw material are weighed and mixed, and raw material includes the one or more in corn, soya bean, potato, glutinous millet.
Wherein, above-mentioned each raw material can not only provide the protein and polysaccharide of abundance in the cultivating process of biological bacteria,
And cultivate after caused slag charge can also directly dispenser into soil, improved soil structure.It is and of the prior art using high
In the method for sugared raw material cultivation biological bacteria, caused slag charge is easily degenerated, and can not be recycled.
Alternatively, raw material includes corn and soya bean, exemplarily, weighs 10~20 parts of corn in step S1 ', and soya bean 25~
35 parts, above-mentioned number is by weight.
Step S2 ', mixed raw material is handled.
After being handled in step S2 ' mixed raw material, carbon, nitrogen in raw material separate out, and the polysaccharide in raw material
Also it is decomposed.Alternatively, step S2 ' is specifically included：Mixed raw material is steamed or boiled, and it is cool to be dried in the air.Further
Ground, will steam or well-done raw material dries in the air cool to less than 40 DEG C, such as dry in the air to room temperature.Further, to weigh corn in step S1 '
10~20 parts, exemplified by 25~35 parts of soya bean, when being boiled to mixed raw material, the fraction of added water is 50~70 parts.
Exemplarily, the operating process boiled to mixed raw material is as follows：By raw material and water according to certain weight
It is uniformly mixed and heats into heating container (such as heating kettle) than adding, mixing speed is 80~100r/min, heating
Temperature is 90~96 DEG C, and the heat time is 10~20min.
In addition, in the embodiment of the present invention, between step S1 ' and step S2 ', powder can also be carried out to mixed raw material
It is broken, to cause the particle diameter of raw material to reduce, Particle Breakage, the efficiency and effect of subsequent treatment can be effectively improved.Exemplarily, originally
Selected in inventive embodiments, the particle diameter of corn and soya bean after crushing is 40~100 mesh.Alternatively, by raw material with ultramicro grinding
Machine is crushed, and the amount of the raw material crushed every time can be selected according to the capacity of micronizer, and micronizer rotating speed is
5000~10000r/min, grinding time are 1~5min, obtain material powder.
In the embodiment of the present invention, between step S1 ' and step S2 ', ultraviolet kill can also be carried out to mixed raw material
Bacterium is handled, and eliminates the miscellaneous bacteria in raw material, can be advantageous to improve the quality for the biological bacteria cultivated, and will not also during sterilization
Infringement is produced to the nutrient of the nutriments such as polysaccharide.Alternatively, raw material (for example, soya bean, corn) is placed in and dries sealing appearance
Ultraviolet-sterilization is carried out in device, sterilization temperature is 15~25 DEG C, and sterilizing time is 20~40min.
You need to add is that between step S1 ' and step S2 ', mixed raw material is crushed and to mixing
Rear raw material carries out the step of ultraviolet-sterilization processing, can select an execution, can also both be carried out, when both are carried out,
The sequencing of the two preferably first crushes without limiting in the embodiment of the present invention to mixed raw material, then to mixing
Raw material afterwards carries out ultraviolet-sterilization processing, so that ultraviolet-sterilization processing has more preferable effect.
The strain of biological bacteria is implanted into step S3 ', raw material after treatment, and is cultivated.
The strain of the biological bacteria of implantation can be selected according to the effect of the service condition and biological bacteria of biological bacteria, can
The strain of the biological bacteria of choosing can be：Bacillus subtilis, bacillus megaterium, bacillus licheniformis, bacillus thuringiensis,
Bacillus laterosporus, colloid bacillus cereus, Streptomyces jingyangensis, mycorrhizal fungi, azotobacter vinelandii, photosynthetic bacteria group, condensation gemma bar
One or more in bacterium, aspergillus oryzae, Paecilomyces lilacinus.
In above-mentioned each biological bacteria, a variety of biological bacterias are mutually promoted, are complementary to one another, and crop can be made to reach the effect of high yield high yield
Fruit.Exemplarily, bacillus megaterium, bacillusmusilaginosiengineering, which coexist, can wait the generation in metabolic process substantial amounts of plant endogenous
Enzyme, absorptivity of the crop to nutrients such as nitrogen, phosphorus, potassium can be significantly improved；Bacillusmusilaginosiengineering, bacillus laterosporus,
Clothing bacillus, which coexists, can promote crop root growth, and fibrous root increases so that plant endogenous enzyme and plant caused by biological bacteria metabolism
Thing growth regulator enters in plant via root system, promotes leaf photosynthesis, and regulation nutrient flows toward fruit, swollen fruit
Effect of increasing production is obvious, compared with applying chemical fertilizer, can increase production 15% -30% in the case where equivalence is put into；Bacillus laterosporus,
Bacillus subtilis, bacillus coagulans, which coexist, can make Vc contents in fruit improve more than 30%, and soluble sugar improves 2-4 degree；
Lactic acid bacteria, lactobacillus acidophilus, bacillus coagulans, bacillus subtilis, which coexist, can improve essential amino acid (lysine in fruit
And methionine), the content of vitamin B complex and unrighted acid etc., fruit is in good taste, storage endurance, and selling price is high；Rice aspergillus,
The decomposition that can accelerate organic substance coexists in clothing bacillus, bacillus subtilis, manufactures available nutrient for crop, provides power,
Continuous cropping poisonous and harmful substance can be decomposed, prevents continuous cropping.
Exemplarily, when the biological bacteria in the embodiment of the present invention is used in seed treatment processes, withered grass gemma can be selected
One or more in bacillus, bacillus megaterium, bacillus licheniformis etc..
Step S4 ', after the completion of cultivating, extract biological bacteria.
Exemplarily, biological bacteria can be extracted by the mode such as fishing for.Slag charge after extraction biological bacteria can be used for producing
Organic fertilizer or direct dispenser the improved soil structure into soil.
The raw material used during cultivating biological bacteria using the breeding method of above-mentioned biological bacteria is plant material, such as
One or more in corn, soya bean, potato, glutinous millet, so as to avoid using chemical agent in the prior art as former
A series of problems expected and brought, you can to reduce the cultivation cost of biological bacteria, be not likely to produce living contaminants, effectively lifting is cultivated
The quality of the biological bacteria of gained.
In addition, the strain of the biological bacteria in step S3 ' in the embodiment of the present invention is will obtain from air or soil
Flora, and obtained by being cultivated in the culture medium of specific environment.
In one example, cultivate bacillus subtilis and the concrete mode of bacillus licheniformis is：Respectively by withered grass bud
Spore bacillus, bacillus licheniformis are inserted in respective nutrient agar, under the conditions of temperature is 37 DEG C, inclined-plane culture 16h；
By two media transfers into respective eggplant bottle, the condition of culture is the inclined-plane culture 16h under the conditions of 37 DEG C of temperature；By eggplant
Strain after bottle culture, which is inoculated into the seeding tank containing corresponding bacterium culture medium, to be cultivated, and the condition of culture is that temperature is 37 DEG C,
Throughput is 30m3/ h, seeding tank rotating speed are 175r/min, cultivate 12~16h；By the bacillus subtilis turned out and lichens
Bacillus inserts fermentation cylinder for fermentation, and the temperature of fermentation is 37 DEG C, throughput 350m3/ h, fermentation tank rotating speed are 96r/
Min, fermentation time are 16~20h；Press filtration is carried out to obtained zymotic fluid, collects bacillus subtilis filter cake and lichens bud respectively
Spore bacillus filter cake；The filter cake being collected into is subjected to fluidized drying respectively, dry inlet temperature is 80 DEG C, is consolidated after drying
The bacillus subtilis and bacillus licheniformis of body.
In another example, the concrete mode for cultivating bacillus megaterium is：Bacillus megaterium is inserted into potato
In glucose agar medium, under the conditions of temperature is 33 DEG C, inclined-plane culture 16h；Obtained single bacterium colony is transferred in eggplant bottle,
The temperature for cultivating bacillus megaterium is 33 DEG C, and continuation incubation time is 16h；Strain after eggplant bottle culture is inoculated into containing corresponding
Cultivated in the seeding tank of bacterium culture medium, cultivation temperature is 28 DEG C, throughput 30m3/ h, seeding tank rotating speed are 175r/min,
Cultivate 10~15h；The bacillus megaterium turned out is inserted into fermentation cylinder for fermentation, the temperature of fermentation is 28~30 DEG C, ventilation
Measure as 350m3/ h, fermentation tank rotating speed are 96r/min, and fermentation time is 40~48h；Zymotic fluid is collected by filter press technique, obtained huge
Bacterium anthracoides filter cake；The filter cake being collected into is subjected to fluidized drying respectively, dry inlet temperature is 80 DEG C, after drying
To bacillus megaterium powder.
The foregoing is only a specific embodiment of the invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all be contained
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of organic fertilizer with insect-expelling function based on biological bacteria, it is characterised in that including by biological bacteria and substrate fermentation
The mixture of formation, and biological expelling parasite medicament, wherein, matrix includes string and microbial nutrition composition, and biological bacteria is raw
The weight of thing expelling parasite medicament and matrix ratio is：(1~5)：(10~20)：(20~50).
2. organic fertilizer according to claim 1, it is characterised in that biological expelling parasite medicament includes what is treated through biological bacteria
One or more in tobacco, sweet wormwood, fennel, garlic, pepper.
3. organic fertilizer according to claim 1, it is characterised in that biological bacteria is bacillus subtilis, huge gemma bar
One or more in bacterium, bacillus licheniformis.
4. organic fertilizer according to claim 1, it is characterised in that the string in matrix includes wood chip, rice husk, rice
One or more in chaff, stalk.
5. organic fertilizer according to claim 1, it is characterised in that the microbial nutrition composition in matrix includes animal excreta
Just, the one or more in animal-based protein, plant source protein, single cell protein.
A kind of 6. preparation method of the organic fertilizer with insect-expelling function based on biological bacteria, it is characterised in that including：
Step S1, biological bacteria, biological expelling parasite medicament and matrix are weighed, wherein, biological bacteria, the weight of biological expelling parasite medicament and matrix
Than for：(1~5)：(10~20)：(20~50)；
Step S2, the biological bacteria weighed and matrix are mixed, and fermented；
Step S3, in the mixture for forming biological expelling parasite medicament added to fermentation, the organic fertilizer based on biological bacteria is obtained.
7. preparation method according to claim 6, it is characterised in that the biological bacteria in step S1 is cultivated in the following way
Formed：
Raw material is weighed and mixed, and raw material includes the one or more in corn, soya bean, potato, glutinous millet；
Mixed raw material is handled；
The strain of biological bacteria is implanted into raw material after treatment, and is cultivated；
After the completion of cultivation, biological bacteria is extracted.
8. preparation method according to claim 6, it is characterised in that the biological expelling parasite medicament in step S1 passes through such as lower section
Formula obtains：
Tobacco is soaked with the liquid comprising biological bacteria, and is crushed, and/or, sweet wormwood is soaked with the liquid comprising biological bacteria, and break
It is broken, and/or, fennel is soaked with the liquid comprising biological bacteria, and crush, and/or, garlic is soaked with the liquid comprising biological bacteria,
And crush, and/or, pepper is soaked with the liquid comprising biological bacteria, and crush.
9. preparation method according to claim 6, it is characterised in that be additionally included between step S1 and step S2, to base
Matter carries out high-temperature process, and after killing the bacterium in matrix, matrix is cooled into the fermentation temperature needed for step S2.
10. preparation method according to claim 6, it is characterised in that it is additionally included in after step S3, it is organic to what is obtained
Fertilizer carries out granulation processing.
Priority Applications (1)
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| CN110577423A (en) * | 2019-10-10 | 2019-12-17 | 福建绿洲生化有限公司 | Organic fertilizer starter and preparation method thereof |
| CN111747796A (en) * | 2020-06-10 | 2020-10-09 | 昆明学院 | Functional organic fertilizer for preventing and treating tobacco root knot nematode disease and preparation method thereof |
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Application publication date: 20171219 |