CN107475253A - 微卫星不稳定性位点-bat26位点的检测引物、扩增体系及检测试剂盒 - Google Patents
微卫星不稳定性位点-bat26位点的检测引物、扩增体系及检测试剂盒 Download PDFInfo
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- CN107475253A CN107475253A CN201610403299.4A CN201610403299A CN107475253A CN 107475253 A CN107475253 A CN 107475253A CN 201610403299 A CN201610403299 A CN 201610403299A CN 107475253 A CN107475253 A CN 107475253A
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- 208000032818 Microsatellite Instability Diseases 0.000 title claims abstract description 26
- 230000003321 amplification Effects 0.000 title claims abstract description 15
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 15
- 238000001514 detection method Methods 0.000 title abstract description 38
- 238000000034 method Methods 0.000 claims abstract description 52
- 238000002844 melting Methods 0.000 claims abstract description 23
- 230000008018 melting Effects 0.000 claims abstract description 23
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 230000004087 circulation Effects 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 206010028980 Neoplasm Diseases 0.000 description 19
- 206010009944 Colon cancer Diseases 0.000 description 15
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 11
- 238000012360 testing method Methods 0.000 description 11
- 239000012634 fragment Substances 0.000 description 9
- 108020004414 DNA Proteins 0.000 description 8
- 230000000875 corresponding effect Effects 0.000 description 5
- 108091092878 Microsatellite Proteins 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011529 RT qPCR Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 239000012188 paraffin wax Substances 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000000973 chemotherapeutic effect Effects 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000000205 computational method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000011337 individualized treatment Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000036210 malignancy Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002271 resection Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
组分 | 用量(μL) |
10×Buffer | 2-2.5 |
dNTP | 2-2.5 |
EvaGreen | 1-1.25 |
SEQ ID NO:1引物 | 1-1.25 |
SEQ ID NO:2引物 | 1-1.25 |
Taq酶 | 0.2-0.25 |
无酶水 | 11.8-15 |
DNA模板 | 1-5 |
组分 | 用量(μL) |
10×Buffer | 2.5 |
dNTP | 2.5 |
EvaGreen | 1.25 |
SEQ ID NO:1引物 | 1.25 |
SEQ ID NO:2引物 | 1.25 |
Taq酶 | 0.25 |
无酶水 | 15 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610403299.4A CN107475253B (zh) | 2016-06-08 | 2016-06-08 | 微卫星不稳定性位点-bat26位点的检测引物、扩增体系及检测试剂盒 |
Applications Claiming Priority (1)
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CN201610403299.4A CN107475253B (zh) | 2016-06-08 | 2016-06-08 | 微卫星不稳定性位点-bat26位点的检测引物、扩增体系及检测试剂盒 |
Publications (2)
Publication Number | Publication Date |
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CN107475253A true CN107475253A (zh) | 2017-12-15 |
CN107475253B CN107475253B (zh) | 2020-12-29 |
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CN201610403299.4A Active CN107475253B (zh) | 2016-06-08 | 2016-06-08 | 微卫星不稳定性位点-bat26位点的检测引物、扩增体系及检测试剂盒 |
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CN (1) | CN107475253B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108998553A (zh) * | 2018-08-14 | 2018-12-14 | 西北大学 | 一种快速筛选多态性微卫星位点目标引物的方法及引物 |
CN114182011A (zh) * | 2020-09-14 | 2022-03-15 | 中山大学附属第六医院 | 检测微卫星bat25位点稳定性的引物对、试剂盒及方法 |
CN114182012A (zh) * | 2020-09-14 | 2022-03-15 | 中山大学附属第六医院 | 检测微卫星mono27位点稳定性的引物对、试剂盒及方法 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2005100816A4 (en) * | 2005-09-30 | 2005-11-24 | Gregory Capern | Pacer Remote Heart Rate Monitor |
CN102230004A (zh) * | 2011-06-08 | 2011-11-02 | 北京阅微基因技术有限公司 | 肿瘤细胞微卫星不稳定状态的复合扩增体系及检测试剂盒 |
CN103555843A (zh) * | 2013-11-05 | 2014-02-05 | 上海赛安生物医药科技有限公司 | 结直肠癌微卫星不稳定性扩增体系及其检测试剂盒 |
-
2016
- 2016-06-08 CN CN201610403299.4A patent/CN107475253B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2005100816A4 (en) * | 2005-09-30 | 2005-11-24 | Gregory Capern | Pacer Remote Heart Rate Monitor |
CN102230004A (zh) * | 2011-06-08 | 2011-11-02 | 北京阅微基因技术有限公司 | 肿瘤细胞微卫星不稳定状态的复合扩增体系及检测试剂盒 |
CN103555843A (zh) * | 2013-11-05 | 2014-02-05 | 上海赛安生物医药科技有限公司 | 结直肠癌微卫星不稳定性扩增体系及其检测试剂盒 |
Non-Patent Citations (1)
Title |
---|
RAMUNAS JANAVICIUS等: "Microsatellite Instability Detection by High-Resolution Melting Analysis", 《CLINICAL CHEMISTRY》 * |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108998553A (zh) * | 2018-08-14 | 2018-12-14 | 西北大学 | 一种快速筛选多态性微卫星位点目标引物的方法及引物 |
CN114182011A (zh) * | 2020-09-14 | 2022-03-15 | 中山大学附属第六医院 | 检测微卫星bat25位点稳定性的引物对、试剂盒及方法 |
CN114182012A (zh) * | 2020-09-14 | 2022-03-15 | 中山大学附属第六医院 | 检测微卫星mono27位点稳定性的引物对、试剂盒及方法 |
CN114182012B (zh) * | 2020-09-14 | 2023-09-15 | 中山大学附属第六医院 | 检测微卫星mono27位点稳定性的引物对、试剂盒及方法 |
CN114182011B (zh) * | 2020-09-14 | 2023-09-15 | 中山大学附属第六医院 | 检测微卫星bat25位点稳定性的引物对、试剂盒及方法 |
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CN107475253B (zh) | 2020-12-29 |
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Effective date of registration: 20190521 Address after: 510655 No. 19, Erheng Road, Yuancun, Tianhe District, Guangzhou City, Guangdong Province Applicant after: The Sixth Affiliated Hospital of Sun Yat-sen University Address before: 510655 Sixth Hospital Affiliated to Sun Yat-sen University, 26 Yuancun Erheng Road, Tianhe District, Guangzhou City, Guangdong Province Applicant before: Wang Jianping Applicant before: Wang Lei Applicant before: Fu Xinhui Applicant before: Huang Jinglin Applicant before: Chen Zhiting Applicant before: Lin Hanjie Applicant before: Wang Jingxuan |
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