CN107384902B - 一种麦芽糖转化率提高的海藻糖合酶及其制备方法和应用 - Google Patents
一种麦芽糖转化率提高的海藻糖合酶及其制备方法和应用 Download PDFInfo
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- CN107384902B CN107384902B CN201710776826.0A CN201710776826A CN107384902B CN 107384902 B CN107384902 B CN 107384902B CN 201710776826 A CN201710776826 A CN 201710776826A CN 107384902 B CN107384902 B CN 107384902B
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
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- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/01—Hexosyltransferases (2.4.1)
- C12Y204/01245—Alpha,alpha-trehalose synthase (2.4.1.245)
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- C12Y504/00—Intramolecular transferases (5.4)
- C12Y504/99—Intramolecular transferases (5.4) transferring other groups (5.4.99)
- C12Y504/99016—Maltose alpha-D-glucosyltransferase (5.4.99.16)
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Abstract
Description
酶 | 转化率(%) |
野生酶 | 62.2% |
E289G | 69.7% |
H295N | 70.5% |
M344K | 70.3% |
M367L | 69.6% |
H295N/E289G | 70.4% |
H295N/M344K | 70.9% |
H295N/M367L | 71.3% |
H295N/M344K/M367L | 73.7% |
Claims (7)
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CN201710776826.0A CN107384902B (zh) | 2015-04-28 | 2015-04-28 | 一种麦芽糖转化率提高的海藻糖合酶及其制备方法和应用 |
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CN201510210023.XA CN104789539B (zh) | 2015-04-28 | 2015-04-28 | 一种海藻糖合酶的突变体及其制备方法和应用 |
CN201710776826.0A CN107384902B (zh) | 2015-04-28 | 2015-04-28 | 一种麦芽糖转化率提高的海藻糖合酶及其制备方法和应用 |
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CN107384902A CN107384902A (zh) | 2017-11-24 |
CN107384902B true CN107384902B (zh) | 2019-05-17 |
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CN201510210023.XA Active CN104789539B (zh) | 2015-04-28 | 2015-04-28 | 一种海藻糖合酶的突变体及其制备方法和应用 |
CN201710776826.0A Active CN107384902B (zh) | 2015-04-28 | 2015-04-28 | 一种麦芽糖转化率提高的海藻糖合酶及其制备方法和应用 |
CN201710776847.2A Active CN107384903B (zh) | 2015-04-28 | 2015-04-28 | 一种海藻糖合酶突变体及其在制备海藻糖中的应用 |
CN201710776849.1A Active CN107446900B (zh) | 2015-04-28 | 2015-04-28 | 一种海藻糖合酶及其制备方法和应用 |
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CN201710776849.1A Active CN107446900B (zh) | 2015-04-28 | 2015-04-28 | 一种海藻糖合酶及其制备方法和应用 |
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Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105524936B (zh) * | 2016-02-02 | 2018-11-06 | 齐鲁工业大学 | 一种突变的海藻糖合酶及其表达基因与应用 |
CN106399426B (zh) * | 2016-10-10 | 2021-04-30 | 长沙理工大学 | 一种利用镉米生产海藻糖的方法 |
CN110628741B (zh) * | 2017-09-13 | 2021-01-29 | 江南大学 | 麦芽寡糖基海藻糖合成酶突变体及其应用 |
CN108753747B (zh) * | 2018-06-05 | 2021-02-26 | 江南大学 | 一种热稳定性和海藻糖产量提高的MTSase突变体 |
CN108753746B (zh) * | 2018-06-05 | 2021-03-30 | 江南大学 | 一种热稳定性提高的麦芽寡糖基海藻糖合成酶突变体 |
CN111500566B (zh) * | 2019-01-28 | 2022-02-15 | 江南大学 | 一种海藻糖合成酶突变体及其制备方法和应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101629166A (zh) * | 2009-08-20 | 2010-01-20 | 南宁中诺生物工程有限责任公司 | 谷氨酸棒杆菌海藻糖合成酶突变体及其应用 |
CN103421761A (zh) * | 2013-08-23 | 2013-12-04 | 南宁中诺生物工程有限责任公司 | 一种耐热海藻糖合成酶融合酶突变体基因及其应用 |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101629166A (zh) * | 2009-08-20 | 2010-01-20 | 南宁中诺生物工程有限责任公司 | 谷氨酸棒杆菌海藻糖合成酶突变体及其应用 |
CN103421761A (zh) * | 2013-08-23 | 2013-12-04 | 南宁中诺生物工程有限责任公司 | 一种耐热海藻糖合成酶融合酶突变体基因及其应用 |
Non-Patent Citations (1)
Title |
---|
谷氨酸棒杆菌(Corynebacterium glutamicum)合成酶的定点突变及其酶学性质研究;朱绮霞 等;《广西科学》;20121231;第19卷(第2期);第169-173页 |
Also Published As
Publication number | Publication date |
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CN107384903B (zh) | 2019-05-17 |
CN104789539B (zh) | 2017-10-31 |
CN107446900B (zh) | 2019-05-17 |
CN107446900A (zh) | 2017-12-08 |
CN104789539A (zh) | 2015-07-22 |
CN107384903A (zh) | 2017-11-24 |
CN107384902A (zh) | 2017-11-24 |
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Inventor after: Wu Jing Inventor after: Xie Yanping Inventor before: Wu Jing Inventor before: Su Lingqia Inventor before: Zhang Yue |
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Denomination of invention: A Trehalose Synthase with Improved Maltose Conversion and Its Preparation and Application Effective date of registration: 20221008 Granted publication date: 20190517 Pledgee: Agricultural Bank of China Limited by Share Ltd. Leiyang branch Pledgor: Hunan Jindai Technology Development Co.,Ltd. Registration number: Y2022980017598 |
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