CN107382604A - Organic microbial fertilizer of the analogies containing SOD and preparation method and application - Google Patents

Organic microbial fertilizer of the analogies containing SOD and preparation method and application Download PDF

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Publication number
CN107382604A
CN107382604A CN201710586980.1A CN201710586980A CN107382604A CN 107382604 A CN107382604 A CN 107382604A CN 201710586980 A CN201710586980 A CN 201710586980A CN 107382604 A CN107382604 A CN 107382604A
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sod
fertilizer
analogies
value
organic
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赵绘
慕向宾
高鹏
王古林
杜鹏飞
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Daqing High-Tech Zone Huamei Science & Technology Co Ltd
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Daqing High-Tech Zone Huamei Science & Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The present invention relates to organic microbial fertilizer of a kind of analogies containing SOD and preparation method and application, the raw material components of fertilizer include SOD analogies, and the mass ratio of SOD analogies and fertilizer is (0.001~50):100;SOD analogies include metalloproteinases, and metallic element includes:One or more in transition metal, alkali metal and alkali earth metal.Organic microbial fertilizer provided by the invention uses unconventional formula, and SOD analogies are applied in fertilizer, can not only effectively control the growing way of crops early stage, promotes crops restraining the growth of seedlings, takes root and bloom;Grow up the later stage in crop, can preferably promote the accumulation of dry in Fruit, colouring and fruit so that increasing crop yield;And the crops that cultivation obtains have the anti-adversity abilities such as excellent anti-aging, cold-resistant, drought resisting.In addition, method for preparing fertilizer provided by the invention is simple, cost is cheap, it is with a wide range of applications.

Description

Organic microbial fertilizer of the analogies containing SOD and preparation method and application
Technical field
The present invention relates to technical field of fertilizers, and in particular to the organic microbial fertilizer and its system of a kind of analogies containing SOD Preparation Method and application.
Background technology
As large agricultural country, China is while agricultural technology develops rapidly along with the sharp increase of fertilizer demand amount.In recent years Come, in agricultural production the administration of fertilizer play more and more important effect, the consumption figure of wherein composite fertilizer is very big, annual approximate number Ten million ton.At present, domestic fertilizer enterprises are given birth to mainly using nitrogen, phosphorus, potassium as raw material using physics or the method for chemical synthesis Produce three element compound fertilizer or complex fertilizer.But no matter producing composite fertilizer using physics or chemical method, all suffer from compound The shortcomings that fertile function is single, utilization rate is low.Research shows that China is low because of utilization rate of fertilizer every year, caused by economic loss nearly thousand Hundred million yuan.This not only causes a large amount of wastes of resource, and the environment for the survival of mankind such as air, water body soil are caused Serious pollution;Even fertilizer can indirectly produce the material to environment and human hazard, and the health and environment to people cause Very big injury.Therefore, scientific matching prepares chemical fertilizer, is effective and reasonable using chemical fertilizer, reduction applications of pesticide amount and reduction environment Pollution etc. has turned into an important topic of the pendulum in agricultural production.
Converted it is well known that superoxide dismutase (SOD) can be catalyzed superoxides as one kind by disproportionated reaction For oxygen and the enzyme of hydrogen peroxide, it is widely present in all kinds of animals, plant and microorganism and collects;It is as a kind of important antioxygen Agent, protection are exposed to the cell in oxygen.SOD enzymes have the oxygen radical of balance body, surpass so as to be avoided that to work as in body Caused adverse reaction during oxygen anion free radical excessive concentration, in radiation proof, anti-aging, anti-inflammatory, suppression tumour and cancer, certainly Body immunization therapy etc. can show the function of uniqueness.
Based on this, how SOD to be effectively applied to agriculture field, produce a kind of energy consumption is low, supplies consumption is small, raw material utilize Rate is high, environmental pollution is small, energy-conserving and environment-protective, production cost are low and can more effectively meet the new-type fertilizer of plant production demand into The Main way researched and developed and tackled key problems for industry.
The content of the invention
For in the prior art the defects of, the present invention is intended to provide a kind of organic microbial fertilizer of analogies containing SOD and Its preparation method and application.The unconventional formula of Fertilizer application provided by the invention, SOD analogies are effectively incorporated into fertilizer, So that the fertilizer being prepared can effectively control Grain Growth Situation, promote crops restraining the growth of seedlings, take root and bloom, promote fruit In fact expand, paint and fruit in dry accumulation so that increasing crop yield;And the crops that cultivation obtains have excellent resist The anti-adversity abilities such as aging, cold-resistant, drought resisting.
Therefore, the present invention provides following technical scheme:
In a first aspect, the present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, the raw material components bag of fertilizer Include:SOD analogies, and the mass ratio of SOD analogies and fertilizer is (0.001~50):100.
In the further embodiment of the present invention, SOD analogies include metalloproteinases SODm;Wherein, in SODm Metallic element includes:One or more in transition metal, alkali metal and alkali earth metal;It is organic in SODm Part includes artificial synthesized homologous small peptide, and homologous small peptide is by amino acid monomer alanine, leucine, isoleucine, asparagus fern Propylhomoserin, glutamic acid, lysine, arginine, glycine, serine, threonine, asparagine or each auto polymerization of glutamine and Into.SODm of the present invention molecular weight is all possible between being in 1000~2000.
In the further embodiment of the present invention, metallic element in SODm include one kind in La, Nd, Eu and Yb or It is a variety of;Organic ligand includes L-Aspartic acid, Pidolidone or 1B.
The present invention further embodiment in, the raw material components of fertilizer by weight, in addition to:Organic matter 50~ 80 parts by weight, the parts by weight of PTPP 4~5, the parts by weight of three (3- hydroxypropyls) phosphine 6~8 and the parts by weight of potassium sorbate 5~9. Specifically, organic matter may be selected from one or more of in dregs of beans, the chaff dregs of rice, plant ash, chicken manure, humic acid, silkworm excrement and wormcast.
Second aspect, the present invention provide a kind of preparation method of the organic microbial fertilizer of the analogies containing SOD, including following Step:S101:Each raw material components in addition to SOD compounds are simulated are well mixed by the way of ball milling, add water simultaneously afterwards It is 4.8~5.2 to adjust pH value;S102:Simulation SOD compounds are added in the product that S101 is obtained and are well mixed, are adjusted simultaneously It is 6.3~6.5 to save pH value, obtains bacterial manure.It should be noted that the bacterial manure of the present invention can be prepared into solid according to being actually needed State or liquid form.
In the further embodiment of the present invention, in S101, the condition of ball milling is specially:Rotating speed be 350rpm~ 450rpm, Ball-milling Time are 5h~8h;The quality for adding water is 1~2 times of each raw material components gross mass.
In the further embodiment of the present invention, in S102, regulation pH value also includes after being 6.3~6.5:In pH value Fermented to add composite bacteria agent in 6.3~6.5 product;Wherein, composite bacteria agent includes bacillus subtilis and production protein is false Silk yeast.
In the further embodiment of the present invention, addition composite bacteria agent carries out fermentation and specifically included:PH value be 6.3~ Candida utili in 6.5 product after inoculation activation, and so that the content of candida utili is (3~5) × 106cfu/ M, ferment 50min~100min at 28 DEG C~30 DEG C afterwards;Then activation is inoculated with the product after the 50min~100min that ferments Bacillus subtilis afterwards, and so that the content of bacillus subtilis is (1~2) × 105Cfu/m, afterwards at 31 DEG C~32 DEG C Ferment 5h~10h.
In the further embodiment of the present invention, in S102, add simulation SOD compounds and be well mixed, adjust simultaneously Section pH value specifically includes for 6.3~6.5:1/2 simulation SOD compounds are added, regulation pH value is 6.30~6.38;Afterwards in pH value In 6.30~6.38 product, to add 1/4 simulation SOD compounds, regulation pH value is 6.45~6.48;Then it is in pH value In 6.45~6.48 product, remaining simulation SOD compounds are added, regulation pH value is 6.40~6.45.
The third aspect, the organic microbial fertilizer of the analogies provided by the invention containing SOD add in Crop fertilizer or fertilizer Add the application in agent.
Above-mentioned technical proposal provided by the invention has advantages below:
(1) applicant has found by numerous studies:The unconventional formula of Fertilizer application provided by the invention, by SOD analogies Effectively it is incorporated into fertilizer, so that the fertilizer being prepared can effectively control Grain Growth Situation, promote crops to restrain the growth of seedlings, Take root and bloom, the accumulation of dry in promotion Fruit, colouring and fruit so that increasing crop yield;And cultivate what is obtained Crops have the anti-adversity abilities such as excellent anti-aging, cold-resistant, drought resisting.
(2) using the organic microbial fertilizer of the analogies provided by the invention containing SOD, plant growth mistake can effectively be supplemented Various elements and organic matter needed for journey, so that leaf dark green, thickening, strengthen the resistance of plant, robust plant;And The organic microbial fertilizer of the analogies provided by the invention containing SOD can efficient prevention and control continuous cropping soil-borne disease, it can penetrate into soil, Pernicious bacteria and crop contact are blocked, forms protection wall, and kills harmful bacteria, purification soil, avoids soil-borne disease, simultaneously Strengthen immunity of the plant in developmental process.In addition, fertilizer provided by the invention can chesson, break soil hardening, So as to play permeation, promote root growth.
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description Obtain substantially, or recognized by the practice of the present invention.
Embodiment
The embodiment of technical solution of the present invention will be described in detail below.Following examples are only used for clearer Explanation technical scheme, therefore be only used as example, and can not be limited the scope of the invention with this.
Experimental method in following embodiments, it is conventional method unless otherwise specified.
Test material used, is to be commercially available from conventional reagent shop unless otherwise specified in following embodiments.
Quantitative test in following examples, it is respectively provided with and repeats to test three times, data is to repeat the average value of experiment three times Or mean+SD.
Species Bacillus subtilis used in the present invention is that Bacillus subtilis subsp.subtilis (protect by strain Tibetan number:CGMCC 1.1700), strain candida utili is Candida utilis (culture presevation number:CGMCC 2.1005).
The present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, and the raw material components of fertilizer include:SOD is simulated Thing, and the mass ratio of SOD analogies and fertilizer is (0.001~50):100.Wherein, SOD analogies include metalloproteinases SODm;Wherein, the metallic element in SODm includes:One kind in transition metal, alkali metal and alkali earth metal It is or a variety of;Organic ligand in SODm includes artificial synthesized homologous small peptide, and homologous small peptide is by amino acid monomer alanine, bright Propylhomoserin, isoleucine, aspartic acid, glutamic acid, lysine, arginine, glycine, serine, threonine, asparagine or paddy Glutamine is each polymerized.Specifically, SOD analogies of the present invention are prepared using conventional method of the prior art.
Preferably, the raw material components of fertilizer by weight, in addition to:The parts by weight of organic matter 50~80, PTPP 4 ~5 parts by weight, the parts by weight of three (3- hydroxypropyls) phosphine 6~8 and the parts by weight of potassium sorbate 5~9.Specifically, organic matter is selected from beans It is one or more of in the dregs of rice, the chaff dregs of rice, plant ash, chicken manure, humic acid, silkworm excrement and wormcast.
In addition, the present invention specially devises the method for preparing fertilizer, comprise the following steps:
S101:Each raw material components in addition to SOD compounds are simulated are well mixed by the way of ball milling, add water afterwards And pH value is adjusted as 4.8~5.2.Wherein, the condition of ball milling is specially:Rotating speed is 350rpm~450rpm, Ball-milling Time 5h ~8h;The quality for adding water is 1~2 times of each raw material components gross mass.
S102:Simulation SOD compounds are added in the product that S101 is obtained and are well mixed, while adjust pH value as 6.3 ~6.5.Wherein, adjusting after pH value is 6.3~6.5 also includes:Composite bacteria agent is added in the product that pH value is 6.3~6.5 Fermented;Composite bacteria agent includes bacillus subtilis and candida utili.Addition composite bacteria agent carries out fermentation and specifically included: Candida utili in the product that pH value is 6.3~6.5 after inoculation activation, and so that the content of candida utili is (3 ~5) × 106Cfu/m, ferment 50min~100min at 28 DEG C~30 DEG C afterwards;Then after the 50min~100min that ferments Bacillus subtilis in product after inoculation activation, and so that the content of bacillus subtilis is (1~2) × 105Cfu/m, it Ferment 5h~10h at 31 DEG C~32 DEG C afterwards.In addition, adding simulation SOD compounds and being well mixed, while pH value is adjusted as 6.3 ~6.5 specifically include:1/2 simulation SOD compounds are added, regulation pH value is 6.30~6.38;Afterwards pH value be 6.30~ In 6.38 product, 1/4 simulation SOD compounds are added, regulation pH value is 6.45~6.48;Then it is 6.45~6.48 in pH value Product in, add remaining simulation SOD compounds, regulation pH value is 6.40~6.45, obtains fertilizer.
Illustrated with reference to embodiment:
Embodiment one
The present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, and the raw material components of fertilizer include:The weight of dregs of beans 50 Part, the parts by weight of PTPP 5, the parts by weight of three (3- hydroxypropyls) phosphine 6 and the parts by weight of potassium sorbate 9 and SOD analogies are measured, And the mass ratio of SOD analogies and fertilizer is 0.001:100.Wherein, SOD analogies are metalloproteinases SODm, and SODm In metallic element be La, organic ligand is the homologous small peptide that Pidolidone auto polymerization forms.
By above-mentioned raw materials component, using the preparation method of fertilizer provided by the invention, the organic micro- of the analogies containing SOD is prepared Bio-bacterial manure:
S101:By each raw material components in addition to SOD compounds are simulated rotating speed be 350rpm under conditions of ball milling 8h, it 2 times of water for accounting for each raw material components gross mass is added afterwards, and adjusts pH value as 4.8.
S102:Simulation SOD compounds are added in the product that S101 is obtained and are well mixed, while adjust pH value as 6.3; Candida utili in the product that pH value is 6.3 after inoculation activation, and cause the content of candida utili for 5 × 106Cfu/m, ferment 100min at 28 DEG C afterwards;Then the withered grass gemma in the product after the 100min that ferments after inoculation activation Bacillus, and so that the content of bacillus subtilis is 2 × 105Cfu/m, ferment 5h at 32 DEG C afterwards, obtains fertilizer.
Embodiment two
The present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, and the raw material components of fertilizer include:The weight of dregs of beans 80 Part, the parts by weight of PTPP 4, the parts by weight of three (3- hydroxypropyls) phosphine 8 and the parts by weight of potassium sorbate 5 and SOD analogies are measured, And the mass ratio of SOD analogies and fertilizer is 1:100.Wherein, SOD analogies are metalloproteinases SODm, and in SODm Metallic element is Eu, and organic ligand is the homologous small peptide that 1B auto polymerization forms.
By above-mentioned raw materials component, using the preparation method of fertilizer provided by the invention, the organic micro- of the analogies containing SOD is prepared Bio-bacterial manure:
S101:By each raw material components in addition to SOD compounds are simulated rotating speed be 450rpm under conditions of ball milling 5h, it 1 times of water for accounting for each raw material components gross mass is added afterwards, and adjusts pH value as 5.2.
S102:Simulation SOD compounds are added in the product that S101 is obtained and are well mixed, while adjust pH value as 6.5; Candida utili in the product that pH value is 6.5 after inoculation activation, and cause the content of candida utili for 3 × 106Cfu/m, ferment 50min at 30 DEG C afterwards;Then the bacillus subtilis in the product after the 50min that ferments after inoculation activation Bacterium, and so that the content of bacillus subtilis is 1 × 105Cfu/m, ferment 10h at 31 DEG C afterwards, obtains fertilizer.
Embodiment three
The present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, and the raw material components of fertilizer include:The weight of dregs of beans 80 Part, the parts by weight of PTPP 4, the parts by weight of three (3- hydroxypropyls) phosphine 8 and the parts by weight of potassium sorbate 5 and SOD analogies are measured, And the mass ratio of SOD analogies and fertilizer is 1:100.Wherein, SOD analogies are metalloproteinases SODm, and in SODm Metallic element is Eu, and organic ligand is the homologous small peptide that 1B auto polymerization forms.
By above-mentioned raw materials component, using the preparation method of fertilizer provided by the invention, the organic micro- of the analogies containing SOD is prepared Bio-bacterial manure:
S101:By each raw material components in addition to SOD compounds are simulated rotating speed be 450rpm under conditions of ball milling 5h, it 1 times of water for accounting for each raw material components gross mass is added afterwards, and adjusts pH value as 5.2.
S102:Simulation SOD compounds are added in the product that S101 is obtained and are well mixed, while it is 6.5 to adjust pH value, Obtain fertilizer.
Example IV
The present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, and the raw material components of fertilizer include:The weight of dregs of beans 80 Part, the parts by weight of PTPP 4, the parts by weight of three (3- hydroxypropyls) phosphine 8 and the parts by weight of potassium sorbate 5 and SOD analogies are measured, And the mass ratio of SOD analogies and fertilizer is 1:100.Wherein, SOD analogies are metalloproteinases SODm, and in SODm Metallic element is Eu and La, and organic ligand is the homologous small peptide that 1B auto polymerization forms.
By above-mentioned raw materials component, using the preparation method of fertilizer provided by the invention, the organic micro- of the analogies containing SOD is prepared Bio-bacterial manure:
S101:By each raw material components in addition to SOD compounds are simulated rotating speed be 450rpm under conditions of ball milling 5h, it 1 times of water for accounting for each raw material components gross mass is added afterwards, and adjusts pH value as 5.2.
S102:Simulation SOD compounds are added in the product that S101 is obtained and are well mixed, while adjust pH value as 6.5; Candida utili in the product that pH value is 6.5 after inoculation activation, and cause the content of candida utili for 3 × 106Cfu/m, ferment 50min at 30 DEG C afterwards;Then the bacillus subtilis in the product after the 50min that ferments after inoculation activation Bacterium, and so that the content of bacillus subtilis is 1 × 105Cfu/m, ferment 10h at 31 DEG C afterwards, obtains fertilizer.
Embodiment five
The present invention provides a kind of organic microbial fertilizer of the analogies containing SOD, and the raw material components of fertilizer include:The weight of dregs of beans 80 Part, the parts by weight of PTPP 4, the parts by weight of three (3- hydroxypropyls) phosphine 8 and the parts by weight of potassium sorbate 5 and SOD analogies are measured, And the mass ratio of SOD analogies and fertilizer is 1:100.Wherein, SOD analogies are metalloproteinases SODm, and in SODm Metallic element is Eu, and organic ligand is the homologous small peptide that 1B auto polymerization forms.
By above-mentioned raw materials component, using the preparation method of fertilizer provided by the invention, the organic micro- of the analogies containing SOD is prepared Bio-bacterial manure:
S101:By each raw material components in addition to SOD compounds are simulated rotating speed be 450rpm under conditions of ball milling 5h, it 1 times of water for accounting for each raw material components gross mass is added afterwards, and adjusts pH value as 5.2.
S102:1/2 simulation SOD compounds are added in the product that S101 is obtained, regulation pH value is 6.35;Afterwards in pH value In 6.35 product, to add 1/4 simulation SOD compounds, regulation pH value is 6.48;Then in the product that pH value is 6.48, Remaining simulation SOD compounds are added, regulation pH value is 6.45.Candida utilis in the product that pH value is 6.45 after inoculation activation Yeast, and so that the content of candida utili is 3 × 106Cfu/m, ferment 50min at 30 DEG C afterwards;Then fermenting Bacillus subtilis in product after 50min after inoculation activation, and so that the content of bacillus subtilis is 1 × 105cfu/ M, ferment 10h at 31 DEG C afterwards, obtains fertilizer.
In addition, the effect of in order to preferably evaluate each embodiment fertilizer, carry out following test:
First, in each embodiment fertilizer active ingredient measure
The content of active ingredient in each embodiment fertilizer is determined according to NY 884-2012.Specifically, each implementation is detected respectively Living bacteria count and the content of organic matter feelings when fertilizer places (normal temperature avoid light place) 1 year and 2 years in example and comparative example Condition, detection data difference are as shown in table 1.
Living bacteria count and the content of organic matter in each embodiment fertilizer of table 1
2nd, effect detection of each embodiment fertilizer to corn growth situation
Specifically, it is experimental subjects from corn.According to the suitable Experimental Base of corn growth environmental selection, 10m is selected 6 experiment sample prescriptions are chosen altogether, each sample prescription chooses 100 corns in × 10m field as experiment sample prescription.Test sample prescription 1~5 Using the fertilizer in embodiment one to embodiment five, experiment sample prescription 6 applies commercially available corn fertilizer, as a control group.Observation is beautiful The upgrowth situation of rice, concrete outcome are as shown in table 2.
Influence list of each embodiment fertilizer of table 2 to corn growth situation
3rd, effect detection of each embodiment fertilizer to fruit tree growth situation
Specifically, it is experimental subjects from apple tree.According to the suitable Experimental Base of Apple Growth environmental selection, selection 6 experiment sample prescriptions are chosen altogether, each sample prescription chooses 10 fruit trees in 10m × 10m field as experiment sample prescription.Experiment sample prescription 1~ 5 apply the fertilizer in embodiment one to embodiment five, and experiment sample prescription 6 applies commercially available special fertilizer for fruit trees, as a control group.For Each experiment sample prescription, 100 apples are randomly selected same day as experimental subjects in ripe apples season, count its size, It is specific as shown in table 3:
Influence of each embodiment fertilizer of table 3 to apple growing way
Certainly, except the situation that embodiment one is enumerated to embodiment five, the species and percentage by weight of other raw material components, Fermentation condition etc. is also possible.
The unconventional formula of Fertilizer application provided by the invention, SOD analogies are effectively incorporated into fertilizer, so that system Standby obtained fertilizer can effectively control Grain Growth Situation, promote crops restraining the growth of seedlings, take root and bloom, promote Fruit, on The accumulation of dry in color and fruit so that increasing crop yield;And the crops that cultivation obtains have excellent anti-aging, resisted The anti-adversity abilities such as cold, drought resisting.
In the description of the invention, it is to be understood that term " first ", " second " are only used for describing purpose, and can not It is interpreted as indicating or implies relative importance or imply the quantity of the technical characteristic indicated by indicating.Thus, define " the One ", one or more this feature can be expressed or be implicitly included to the feature of " second ".In the description of the invention, " multiple " are meant that two or more, unless otherwise specifically defined.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area Art personnel can be tied the different embodiments or example and the feature of different embodiments or example described in this specification Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned Embodiment is changed, changed, replacing and modification.

Claims (10)

  1. A kind of 1. organic microbial fertilizer of analogies containing SOD, it is characterised in that:
    The raw material components of the fertilizer include:SOD analogies, and the mass ratio of the SOD analogies and the fertilizer is (0.001~50):100.
  2. 2. the organic microbial fertilizer of the analogies according to claim 1 containing SOD, it is characterised in that:
    The SOD analogies include metalloproteinases SODm;
    Wherein, the metallic element in the SODm includes:One in transition metal, alkali metal and alkali earth metal Kind is a variety of;
    Organic ligand in the SODm includes artificial synthesized homologous small peptide, and the homologous small peptide is by the ammonia of amino acid monomer third Acid, leucine, isoleucine, aspartic acid, glutamic acid, lysine, arginine, glycine, serine, threonine, asparagus fern acyl Amine or glutamine are each polymerized.
  3. 3. the organic microbial fertilizer of the analogies according to claim 2 containing SOD, it is characterised in that
    Metallic element in the SODm includes the one or more in La, Nd, Eu and Yb;The organic ligand includes L- asparagus ferns Propylhomoserin, Pidolidone or 1B.
  4. 4. the organic microbial fertilizer of the analogies according to claim 3 containing SOD, it is characterised in that the original of the fertilizer Expect component by weight, in addition to:
    The parts by weight of organic matter 50~80, the parts by weight of PTPP 4~5, the parts by weight of three (3- hydroxypropyls) phosphine 6~8 and sorb The sour parts by weight of potassium 5~9.
  5. 5. the preparation method of the organic microbial fertilizer of the analogies containing SOD described in any one of Claims 1 to 4, it is characterised in that Comprise the following steps:
    S101:Each raw material components in addition to SOD compounds are simulated are well mixed by the way of ball milling, adds water afterwards and adjusts It is 4.8~5.2 to save pH value;
    S102:Simulation SOD compounds are added in the product that the S101 is obtained and are well mixed, while adjust pH value as 6.3 ~6.5, obtain the bacterial manure.
  6. 6. the preparation method of the organic microbial fertilizer of the analogies according to claim 5 containing SOD, it is characterised in that:
    In the S101, the condition of the ball milling is specially:Rotating speed is 350rpm~450rpm, and Ball-milling Time is 5h~8h;Add The quality for entering water is 1~2 times of each raw material components gross mass.
  7. 7. the preparation method of the organic microbial fertilizer of the analogies according to claim 5 containing SOD, it is characterised in that:
    In the S102, the regulation pH value also includes after being 6.3~6.5:
    Composite bacteria agent is added in the product that the pH value is 6.3~6.5 to be fermented;Wherein, the composite bacteria agent includes withered Careless bacillus and candida utili.
  8. 8. the preparation method of the organic microbial fertilizer of the analogies according to claim 7 containing SOD, it is characterised in that:
    The addition composite bacteria agent carries out fermentation and specifically included:
    Candida utili in the product that the pH value is 6.3~6.5 after inoculation activation, and cause the Candida utilis ferment Female content is (3~5) × 106Cfu/m, ferment 50min~100min at 28 DEG C~30 DEG C afterwards;Then in the fermentation Bacillus subtilis in product after 50min~100min after inoculation activation, and cause the content of the bacillus subtilis For (1~2) × 105Cfu/m, ferment 5h~10h at 31 DEG C~32 DEG C afterwards.
  9. 9. the preparation method of the organic microbial fertilizer of the analogies according to claim 5 containing SOD, it is characterised in that:
    In the S102, add simulation SOD compounds and be well mixed, while adjust pH value and specifically included for 6.3~6.5:
    The 1/2 simulation SOD compounds are added, regulation pH value is 6.30~6.38;It is afterwards 6.30~6.38 in the pH value Product in, add the 1/4 simulation SOD compounds, regulation pH value is 6.45~6.48;Then the pH value be 6.45~ In 6.48 product, the remaining simulation SOD compounds are added, regulation pH value is 6.40~6.45.
  10. 10. the organic microbial fertilizer of the analogies containing SOD described in any one of Claims 1 to 4 is in Crop fertilizer or fertilizer Application in additive.
CN201710586980.1A 2017-07-18 2017-07-18 Organic microbial fertilizer of the analogies containing SOD and preparation method and application Pending CN107382604A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476643A (en) * 2019-09-05 2019-11-22 天津核生科技生物工程有限公司 A method of fruits and vegetables are planted using SOD fruit vegetable nutrient agent

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CN102351611A (en) * 2011-06-28 2012-02-15 蔡明� Soil improvement regulator and its preparation method
CN106396829A (en) * 2015-07-31 2017-02-15 湖北宏全生物科技有限公司 Production method for biofertilizer with SOD enzyme activity

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Publication number Priority date Publication date Assignee Title
CN102351611A (en) * 2011-06-28 2012-02-15 蔡明� Soil improvement regulator and its preparation method
CN106396829A (en) * 2015-07-31 2017-02-15 湖北宏全生物科技有限公司 Production method for biofertilizer with SOD enzyme activity

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476643A (en) * 2019-09-05 2019-11-22 天津核生科技生物工程有限公司 A method of fruits and vegetables are planted using SOD fruit vegetable nutrient agent

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Inventor after: Zhao Hui

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Application publication date: 20171124