CN106396829A - Production method for biofertilizer with SOD enzyme activity - Google Patents
Production method for biofertilizer with SOD enzyme activity Download PDFInfo
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- CN106396829A CN106396829A CN201510464187.5A CN201510464187A CN106396829A CN 106396829 A CN106396829 A CN 106396829A CN 201510464187 A CN201510464187 A CN 201510464187A CN 106396829 A CN106396829 A CN 106396829A
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Abstract
The invention discloses a production method for biofertilizer with SOD enzyme activity. The production method comprises the following steps: preparation of SOD bacterial liquid of bacillus; batching; fertilizer granulation; etc. The prepared biofertilizer has the advantages of strong heat resistance, substantial improvement of the utilization rate of fertilizer, reduction in production cost, capacity of overcoming the problems of ageing root attenuation, poor anti-adversity ability and the like of plants, enhancement of resistance of plants, and improvement of product quality and output.
Description
Technical field
The present invention relates to a kind of production method of bio-fertilizer, specifically one kind have SOD enzyme
The production method of the bio-fertilizer of activity, belongs to bio-fertilizer production field.
Background technology
Erythrocuprein (SuperoxideDismutase abbreviation SOD) is a kind of novel enzyme system
Agent.It is wide in the distributed pole of living nature, almost from animal to plant, or even from people to unicellular
Biology, has its presence.SOD is considered the enzyme of most magical magic power, people in Life Science
Internal rubbish street cleaner.SOD is the natural enemy of oxygen radical, is oxygen radical in body
Number one killer, be life and health this.SOD level height in vivo means to decline
Old with dead index directly perceived；Now it has proven convenient that the disease being caused by oxygen radical is up to more than 60
Kind.It can resist and block infringement cell being caused because of oxygen radical, and repairs impaired in time
Cell, restore because free radical cause to cell damage.Due to modern life pressure, environment is dirty
Dye, various radiation and excess motion all can cause oxygen radical to be formed in a large number；Therefore, biological anti-
In oxidation mechanism, the status of SOD is more and more important.
Content of the invention
It is an object of the invention to, devise a kind of production of the bio-fertilizer with SOD enzyme activity
Method, has stronger temperature capacity, and chemical fertilizer utilization ratio has both been significantly increased, and reduces and produces into
This, overcome the problems such as plant senescence, root system reduction, anti-adversity ability difference, strengthen plant resistant power,
Also improve product quality, improve yield.
The technical scheme is that：
A kind of production method of the bio-fertilizer with SOD enzyme activity, comprises the following steps：
(1) preparation of bud pole bacterium SOD bacterium solution：Bud pole bacterium is trained through first order seed successively
Support, secondary seed spread cultivation and three-level thalline fermented and cultured after, then through 10 tons of fermentation tanks 2-3 days
Culture, 5% fineness then adding bacterium solution weight in the bacterium solution after fermented and cultured is
The turf of 0.1mm or humic acid, and 5% SOD analogies, after sufficiently mixing, utilize
Plate and frame filter press carries out squeezing filter, then to squeeze the solid bacterium material that obtains of filter carry out low temperature drying so that
Final water content is less than or equal to 7%, finally by dried bacterium material through ultra-fine pulverizer disintegrating,
And cross 0.1mm mesh sieve, prepared bacterial content is 5.0 × 1011The SOD preparation of cfu/g；
Wherein, described SOD analogies are selected from：Poly-aspartate iron, poly-aspartate is violent,
L-amino acid-copper (II)-o-phenanthroline type SOD analogies, glycine-nickel-benzo miaow
Azoles type SOD analogies, L-Aspartic acid-nickel-benzimidazole type SOD analogies and L-
One or more of glutamic acid-nickel-benzimidazole type SOD analogies.
(2) dispensing：By npk fertilizer, organic matter, middle trace element, calcium magnesium phosphate and
Fill material is pulverized after being mixed, and can improve mixed effect by pulverizing, when reducing mixing
Between, improve operating efficiency, then 100 mesh sieve, the bud then prepared with step (1) again
Pole bacterium SOD bacterium solution mixes, and controls the pH value of mixed material in 6-6.5；
(3) granulation of fertilizer：Mixed raw material is granulated, obtains bio-fertilizer of the present invention
Finished product.
Described organic matter is selected from：Dregs of beans, the chaff dregs of rice, plant ash, chicken manure, humic acid, silkworm excrement and
In wormcast one or more.
Described middle trace element is selected from：In calcium, magnesium, boron, molybdenum, manganese, zinc, copper, iron and selenium
One or more.
Described filler is selected from：One of diatomite, bentonite, attapulgite and vermiculite or
Several.
Bio-fertilizer of the present invention can make the SOD content of crop significantly improve, and has stronger resistance to
Heat energy power, processes 30min under the conditions of 70 DEG C -100 DEG C, and enzyme activity remains to reach what protoenzyme was lived
30%-45%；Crop alimentary enriches, and has the function of improving body immunity and anti-aging.
It is an advantage of the current invention that：There is stronger temperature capacity, chemical fertilizer profit has both been significantly increased
With rate, reduce production cost, overcome the problems such as plant senescence, root system reduction, anti-adversity ability difference,
Strengthen plant resistant power, also improve product quality, improve yield.
With reference to specific embodiment, the invention will be further described.
Specific embodiment
Hereinafter the preferred embodiments of the present invention are illustrated it will be appreciated that described herein
Preferred embodiment is merely to illustrate and explains the present invention, is not intended to limit the present invention.
Unless otherwise stated, the percentage employed in the present invention is percetage by weight.
Embodiment 1
A kind of production method of the bio-fertilizer with SOD enzyme activity, comprises the following steps：
(1) preparation of bud pole bacterium SOD bacterium solution：Bud pole bacterium is trained through first order seed successively
Support, secondary seed spread cultivation and three-level thalline fermented and cultured after, then through 10 tons of fermentation tanks 2-3 days
Culture, 5% fineness then adding bacterium solution weight in the bacterium solution after fermented and cultured is
The turf of 0.1mm or humic acid, and 5% SOD analogies, after sufficiently mixing, utilize
Plate and frame filter press carries out squeezing filter, then to squeeze the solid bacterium material that obtains of filter carry out low temperature drying so that
Final water content is less than or equal to 7%, finally by dried bacterium material through ultra-fine pulverizer disintegrating,
And cross 0.1mm mesh sieve, prepared bacterial content is 5.0 × 1011The SOD preparation of cfu/g；
Wherein, described SOD analogies are selected from：Poly-aspartate iron, poly-aspartate is violent,
L-amino acid-copper (II)-o-phenanthroline type SOD analogies, glycine-nickel-benzo miaow
Azoles type SOD analogies, L-Aspartic acid-nickel-benzimidazole type SOD analogies and L-
One or more of glutamic acid-nickel-benzimidazole type SOD analogies.
(2) dispensing：By npk fertilizer, organic matter, middle trace element, calcium magnesium phosphate and
Fill material is pulverized after being mixed, and can improve mixed effect by pulverizing, when reducing mixing
Between, improve operating efficiency, then 100 mesh sieve, the bud then prepared with step (1) again
Pole bacterium SOD bacterium solution mixes, and controls the pH value of mixed material in 6-6.5；
(3) granulation of fertilizer：Mixed raw material is granulated, obtains bio-fertilizer of the present invention
Finished product.
Described organic matter is selected from：Dregs of beans, the chaff dregs of rice, plant ash, chicken manure, humic acid, silkworm excrement and
In wormcast one or more.
Described middle trace element is selected from：In calcium, magnesium, boron, molybdenum, manganese, zinc, copper, iron and selenium
One or more.
Described filler is selected from：One of diatomite, bentonite, attapulgite and vermiculite or
Several.
Bio-fertilizer of the present invention can make the SOD content of crop significantly improve, and has stronger resistance to
Heat energy power, processes 30min under the conditions of 70 DEG C -100 DEG C, and enzyme activity remains to reach what protoenzyme was lived
30%-45%；Crop alimentary enriches, and has the function of improving body immunity and anti-aging.
In the present invention, raw materials used being during this area produces commonly uses raw material, all can obtain from market
Arrive, and impact will not be produced for producing result；Various equipment employed in the present invention, all
Conventional equipment used in the production technology of this area, and the operation of each equipment, parameter etc. all press
More solito operation is carried out, and has no special feature.
Claims (4)
1. a kind of production method of the bio-fertilizer with SOD enzyme activity is it is characterised in that wrap
Include following steps：
(1) preparation of bud pole bacterium SOD bacterium solution：Bud pole bacterium is trained through first order seed successively
Support, secondary seed spread cultivation and three-level thalline fermented and cultured after, then through 10 tons of fermentation tanks 2-3 days
Culture, 5% fineness then adding bacterium solution weight in the bacterium solution after fermented and cultured is
The turf of 0.1mm or humic acid, and 5% SOD analogies, after sufficiently mixing, utilize
Plate and frame filter press carries out squeezing filter, then to squeeze the solid bacterium material that obtains of filter carry out low temperature drying so that
Final water content is less than or equal to 7%, finally by dried bacterium material through ultra-fine pulverizer disintegrating,
And cross 0.1mm mesh sieve, prepared bacterial content is 5.0 × 1011The SOD preparation of cfu/g；
Wherein, described SOD analogies are selected from：Poly-aspartate iron, poly-aspartate is violent,
L-amino acid-copper (II)-o-phenanthroline type SOD analogies, glycine-nickel-benzo miaow
Azoles type SOD analogies, L-Aspartic acid-nickel-benzimidazole type SOD analogies and L-
One or more of glutamic acid-nickel-benzimidazole type SOD analogies.
(2) dispensing：By npk fertilizer, organic matter, middle trace element, calcium magnesium phosphate and
Fill material is pulverized after being mixed, and can improve mixed effect by pulverizing, when reducing mixing
Between, improve operating efficiency, then 100 mesh sieve, the bud then prepared with step (1) again
Pole bacterium SOD bacterium solution mixes, and controls the pH value of mixed material in 6-6.5；
(3) granulation of fertilizer：Mixed raw material is granulated, obtains bio-fertilizer of the present invention
Finished product.
2. the production of a kind of bio-fertilizer with SOD enzyme activity according to claim 1
Method it is characterised in that：Described organic matter is selected from：Dregs of beans, the chaff dregs of rice, plant ash, chicken manure,
In humic acid, silkworm excrement and wormcast one or more.
3. the production of a kind of bio-fertilizer with SOD enzyme activity according to claim 1
Method it is characterised in that：Described middle trace element is selected from：Calcium, magnesium, boron, molybdenum, manganese, zinc,
One or more of copper, iron and selenium.
4. the production of a kind of bio-fertilizer with SOD enzyme activity according to claim 1
Method it is characterised in that：Described filler is selected from：Diatomite, bentonite, attapulgite and
One or more of vermiculite.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510464187.5A CN106396829A (en) | 2015-07-31 | 2015-07-31 | Production method for biofertilizer with SOD enzyme activity |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510464187.5A CN106396829A (en) | 2015-07-31 | 2015-07-31 | Production method for biofertilizer with SOD enzyme activity |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107382604A (en) * | 2017-07-18 | 2017-11-24 | 大庆高新区华美科技有限公司 | Organic microbial fertilizer of the analogies containing SOD and preparation method and application |
| CN109574762A (en) * | 2018-09-05 | 2019-04-05 | 陈德茂 | A kind of SOD soil-repairing agent and preparation method thereof and application method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060154821A1 (en) * | 2005-01-13 | 2006-07-13 | National Taiwan University | Bio-fertilizer composition for promoting growth or orchid plants and application |
| CN102557798A (en) * | 2011-12-31 | 2012-07-11 | 山东宝源生物有限公司 | Organic-inorganic compound fertilizer added with SOD (Super Oxygen Dehydrogenise) analogue and production method thereof |
| CN104686270A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Production method for Korla bergamot pears with SOD enzymatic activity |
-
2015
- 2015-07-31 CN CN201510464187.5A patent/CN106396829A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060154821A1 (en) * | 2005-01-13 | 2006-07-13 | National Taiwan University | Bio-fertilizer composition for promoting growth or orchid plants and application |
| CN102557798A (en) * | 2011-12-31 | 2012-07-11 | 山东宝源生物有限公司 | Organic-inorganic compound fertilizer added with SOD (Super Oxygen Dehydrogenise) analogue and production method thereof |
| CN104686270A (en) * | 2015-02-03 | 2015-06-10 | 新疆天物生态科技股份有限公司 | Production method for Korla bergamot pears with SOD enzymatic activity |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107382604A (en) * | 2017-07-18 | 2017-11-24 | 大庆高新区华美科技有限公司 | Organic microbial fertilizer of the analogies containing SOD and preparation method and application |
| CN109574762A (en) * | 2018-09-05 | 2019-04-05 | 陈德茂 | A kind of SOD soil-repairing agent and preparation method thereof and application method |
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Application publication date: 20170215 |