CN107018956A - Standardized breeding method for aedes albopictus larvae - Google Patents
Standardized breeding method for aedes albopictus larvae Download PDFInfo
- Publication number
- CN107018956A CN107018956A CN201710167039.6A CN201710167039A CN107018956A CN 107018956 A CN107018956 A CN 107018956A CN 201710167039 A CN201710167039 A CN 201710167039A CN 107018956 A CN107018956 A CN 107018956A
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- aedes albopictus
- suspension feed
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- feeding
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- 241000256173 Aedes albopictus Species 0.000 title claims abstract description 27
- 238000009395 breeding Methods 0.000 title claims abstract description 11
- 239000000725 suspension Substances 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 24
- 230000007423 decrease Effects 0.000 claims abstract description 3
- 230000000384 rearing effect Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 230000001488 breeding effect Effects 0.000 claims description 9
- 239000000843 powder Substances 0.000 claims description 5
- 241000238557 Decapoda Species 0.000 claims description 4
- 235000021336 beef liver Nutrition 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 3
- 230000003203 everyday effect Effects 0.000 abstract 1
- 241000382353 Pupa Species 0.000 description 35
- 238000000926 separation method Methods 0.000 description 11
- 241000255925 Diptera Species 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 241000256113 Culicidae Species 0.000 description 1
- 208000001490 Dengue Diseases 0.000 description 1
- 206010012310 Dengue fever Diseases 0.000 description 1
- 201000006353 Filariasis Diseases 0.000 description 1
- 208000003152 Yellow Fever Diseases 0.000 description 1
- 235000021405 artificial diet Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 208000025729 dengue disease Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 238000004920 integrated pest control Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 230000001418 larval effect Effects 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000001932 seasonal effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000011425 standardization method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a standardized breeding method of aedes albopictus larvae, which comprises the following steps: when the temperature is 24-28 ℃ and the relative humidity is 60-90%, putting the hatched aedes albopictus larvae into a feeding tray for feeding according to the feeding density of 3000-8000 aedes albopictus larvae per tray, and quantitatively adding suspension feed according to the feeding density every day in the feeding process, wherein the suspension feed amount shows a rule that the time periods of the 4 th day and/or the 5 th day and more than two ends of the middle time period decrease within the feeding time of one week; the invention creates conditions for separating male and female of aedes albopictus pupae, provides powerful guarantee for large-scale production in laboratories and factories, and solves the problem of asynchronous development of aedes albopictus larvae in the feeding process.
Description
Technical field：
The present invention relates to aedes albopictus artificial diet technique field, and in particular to a kind of Aedes Albopictus Larva standardization is raised
Method.
Background technology：
It is one of basic fundamental for entomologizing manually to raise insect, can not be subject to seasonal restrictions to breed certain species
Purpose insect, for insect biology, physiology and integrated pest control measure formulate in terms of research.Mosquito
(mosquito) Insecta Diptera Dulicidae, is belonged to, the whole world there are about 3000 kinds.It is a kind of fine winged with sucking mouth parts
Worm.Generally female is using blood as food, and the juice of male then sucking plant.The female mosquito sucked blood is dengue fever, stockaded village's card, malaria
The vector of the other pathogens such as disease, yellow fever, filariasis, encephalitis B.At present, as mosquito is ecological and Prevention Technique
That studies deepens continuously, more particularly to carrying out in a deep going way, it is necessary to which a large amount of supply for the research work such as biological control and resistance to the action of a drug measure
Test worm source.Therefore, the artificial feeding larvae under laboratory and workshop mass production conditions, standard is provided for scientific experiment
Experiment larva highlighted its significance, and rarely have the correlative study report of the artificial feeding on mosquito larvae both at home and abroad.
With the rearing method of existing nonstandardized technique, survival rate of larvae develops irregular etc. compared with being significantly reduced under its native environmental conditions
The appearance of situation, does not reach the standard compliant larva needed for experiment or production, is not suitable for mass rearing, it is difficult to promote.
The content of the invention：
It can guarantee that Aedes Albopictus Larva normal growth it is an object of the invention to provide a kind of and reach the white of preferable effect
Line albopictus larvae standardizes method for breeding, so that the male and female separation for aedes albopictus tumbler creates condition, it is laboratory and work
The large-scale production of factory provides powerful guarantee, solves the nonsynchronous problem of development in Aedes Albopictus Larva feeding process.
The present invention is achieved by the following technical programs：
A kind of Aedes Albopictus Larva standardizes method for breeding, and this method comprises the following steps：It is 24-28 DEG C, phase in temperature
During to humidity 60-90% conditions, the Aedes Albopictus Larva hatched is put into according to the stocking density of 3000-8000 bars/disk and raised
Support in disk and raise, in feeding process, suspension feed is quantitatively added according to stocking density daily, and suspension feed amount one
Presentation intermediary time period the 4th day and/or the rule that the two ends period successively decreases more than the 5th day in the feeding time in week；When in rearing tray
When the stocking density of Aedes Albopictus Larva is 3000-5000 bars/disk, first day addition 20ml suspension feed does not add on the 2nd day
Plus, the 3rd day addition 30-40ml suspension feed, the 4th day addition 40-60ml suspension feed, the 5th day addition 30-
60ml suspension feed, the 6th day addition 20-40ml suspension feed, the 7th day without；When aedes albopictus in rearing tray
When the stocking density of larva is 6000-8000 bars/disk, first day addition 25-30ml suspension feed, the 2nd day without the
Three days addition 40-50ml suspension feed, fourth, fifth day addition 70-80ml suspension feed, the 6th day addition 40-
50ml suspension feed, the 7th day without；The long 60cm of rearing tray, width 40cm, high 6cm, load the clean of 3-5L
Water, the depth of water about 1cm-2cm；The suspension feed, according to the mass fraction, including 60~84 parts of beef liver powder, 24~36 parts of shrimp med,
12~36 parts of dusty yeast, 2000 parts of water.
Beneficial effects of the present invention are as follows：
The present invention's can meet the normal growth of Aedes Albopictus Larva and reach optimal effect, so as to be aedes albopictus
The male and female separation of tumbler creates condition.Raised by standardizing, its larva survival rate is reached more than 95%；Larva exists
During pupa 45H, pupa rate reaches 60%-80%；The wide size of cephalothorax of male pupa is in 0.95-1.05mm, and the female wide size of pupa cephalothorax exists
1.15-1.30mm, its size for using male and female separation device separation male and female tumbler it is very crucial, its separation when state difference
Substantially, male and female tumbler of female, the male tumbler compartment away between substantially without transition；Very easily male and female tumbler is separated；
The mixed female rate in the male pupa separated by separator will be greatly reduced, the mixed female rate in its male pupa is (male after separation after measured
The ratio for being mixed into female pupa of pupa) also reduced less than the male pupa loss late in 0.1%, and female pupa；Male pupa output capacity is greatly improved, is
Large-scale production lays the foundation, and the present invention solves the technical difficulties such as development in Aedes Albopictus Larva feeding process is asynchronous
Topic, it is ensured that the normal growth and survival rate of larva, the large-scale production for laboratory and factory provide powerful guarantee.
Brief description of the drawings：
Fig. 1 is signal of the standardization method for breeding of embodiment 1 when being separated obtained male pupa and female pupa on the 8th day
Figure；
Fig. 2 is signal of the common method for breeding of comparative example 1 when being separated obtained male pupa and female pupa on the 8th day
Figure.
Embodiment：
Further illustrated the following is to the present invention, rather than limitation of the present invention.
Embodiment 1：
A kind of Aedes Albopictus Larva standardizes method for breeding, and this method comprises the following steps：It is 24-28 DEG C, phase in temperature
During to humidity 60-90% conditions, the Aedes Albopictus Larva hatched is put into according to the stocking density of 3000-8000 bars/disk and raised
Support in disk and raise, in feeding process, suspension feed, and the addition of suspension feed are quantitatively added according to stocking density daily
In the feeding time of one week as shown in table 1, the long 60cm of rearing tray, width 40cm, high 6cm, loading 3-5L clean water,
The depth of water about 1cm-2cm；The suspension feed, according to the mass fraction, including 60~84 parts of beef liver powder, 24~36 parts of shrimp med, ferment
12~36 parts of female powder, 2000 parts of water.Raised by standardizing, larva survival rate can reach more than 95%；Larva is in pupa 45H
When, pupa rate reaches 60%-80%；The wide size of cephalothorax of male pupa is in 0.95-1.05mm, and the female wide size of pupa cephalothorax is in 1.15-
1.30mm, its size is for very crucial using male and female separation device separation male and female tumbler, at the 8th day just very easily by male and female
Tumbler separates, as shown in figure 1, male and female tumbler of female, the male tumbler compartment away between substantially without transition；After measured in its male pupa
The male pupa loss late that is less than in 0.1%, and female pupa of mixed female rate (ratio for being mixed into female pupa of male pupa after separation) also reduce；Significantly
Male pupa output capacity is improved, the requirement of large-scale production and application is reached.
Comparative example 1：
A kind of Aedes Albopictus Larva standardizes method for breeding, and this method comprises the following steps：By Aedes Albopictus Larva in length
Raised in 60cm, wide 40cm, high 6cm cuboid rearing tray；Add the clear water 4L for standing one day in rearing tray.It will hatch
Larval counts and be positioned over according to 6000/disk in rearing tray, in feeding process first day addition 10ml suspension feed,
2nd day addition 10ml suspension feed, the 3rd day addition 30ml suspension feed, the 4th day addition 50ml suspension is raised
Material, five days addition 60ml suspension feed, the 6th day addition 60ml suspension feed, the 7th day without.
The suspension feed, according to the mass fraction, including 60~84 parts of beef liver powder, 24~36 parts of shrimp med, dusty yeast 12
~36 parts, 2000 parts of water.More than 95% can be reached by breeding grub survival rate；Larva is in pupa 45H, and pupa rate reaches
60%-80%；The wide size of cephalothorax of male pupa in 0.95-1.15mm, the female wide size of pupa cephalothorax in 1.05-1.30mm, referring to Fig. 2,
The Individual Size difference of male and female pupa is intersected many in the wide size of cephalothorax, and separating effect of the male and female pupa on separator be not obvious, its
Compartment female, male tumbler of transition away between is more, is difficult to determine the separation point position of female, male tumbler；After measured in its male pupa
Mixed female rate (ratio for being mixed into female pupa of male pupa after separation) is far longer than 2%, and the loss late of male pupa is very high, is unfavorable for big rule
Mould production application.
Claims (1)
1. a kind of Aedes Albopictus Larva standardizes method for breeding, it is characterised in that this method comprises the following steps：It is in temperature
24-28 DEG C, during relative humidity 60-90% conditions, by raising of the Aedes Albopictus Larva hatched according to 3000-8000 bars/disk
Raised in density input rearing tray, in feeding process, suspension feed is quantitatively added according to stocking density daily, and suspension is raised
The rule that intermediary time period the 4th day is presented in the amount of material in the feeding time of one week and/or the two ends period successively decreases more than the 5th day；
When the stocking density of Aedes Albopictus Larva in rearing tray is 3000-5000 bars/disk, first day addition 20ml suspension is raised
Material, the 2nd day without the 3rd day addition 30-40ml suspension feed, the 4th day addition 40-60ml suspension feed, the
Five days addition 30-60ml suspension feed, the 6th day addition 20-40ml suspension feed, the 7th day without；Work as raising
When the stocking density of Aedes Albopictus Larva is 6000-8000 bars/disk in disk, first day addition 25-30ml suspension feed, the
2 days without the 3rd day addition 40-50ml suspension feed, fourth, fifth day addition 70-80ml suspension feed, the 6th
It addition 40-50ml suspension feed, the 7th day without；The long 60cm of rearing tray, width 40cm, high 6cm, load 3-
5L clean water, the depth of water about 1cm-2cm；The suspension feed, according to the mass fraction, including 60~84 parts of beef liver powder, shrimp med
24~36 parts, 12~36 parts of dusty yeast, 2000 parts of water.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710167039.6A CN107018956B (en) | 2017-03-20 | 2017-03-20 | Standardized breeding method for aedes albopictus larvae |
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|---|---|---|---|
| CN201710167039.6A CN107018956B (en) | 2017-03-20 | 2017-03-20 | Standardized breeding method for aedes albopictus larvae |
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| Publication Number | Publication Date |
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| CN107018956A true CN107018956A (en) | 2017-08-08 |
| CN107018956B CN107018956B (en) | 2019-06-21 |
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| CN201710167039.6A Active CN107018956B (en) | 2017-03-20 | 2017-03-20 | Standardized breeding method for aedes albopictus larvae |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372371A (en) * | 2017-09-08 | 2017-11-24 | 广州威佰昆生物科技有限公司 | Breeding method of aedes albopictus oviposition female mosquito |
| CN107372370A (en) * | 2017-09-08 | 2017-11-24 | 广州威佰昆生物科技有限公司 | Hatching solution for hatching aedes albopictus eggs and hatching method of aedes albopictus eggs |
| CN108094768A (en) * | 2017-12-07 | 2018-06-01 | 吉林农业大学 | It cultivates wriggler stalk fermentation matrix and its cultivates wriggler method |
| WO2019041208A1 (en) * | 2017-08-31 | 2019-03-07 | 广州威佰昆生物科技有限公司 | Culex quinquefasciatus and method for obtaining same |
| CN113712137A (en) * | 2021-08-11 | 2021-11-30 | 广州威佰昆生物科技有限公司 | Artificial feed for culex larvae and artificial feeding method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106212855A (en) * | 2016-08-04 | 2016-12-14 | 广州威佰昆生物科技有限公司 | Artificial feed for aedes albopictus larvae and preparation method |
-
2017
- 2017-03-20 CN CN201710167039.6A patent/CN107018956B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106212855A (en) * | 2016-08-04 | 2016-12-14 | 广州威佰昆生物科技有限公司 | Artificial feed for aedes albopictus larvae and preparation method |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019041208A1 (en) * | 2017-08-31 | 2019-03-07 | 广州威佰昆生物科技有限公司 | Culex quinquefasciatus and method for obtaining same |
| CN107372371A (en) * | 2017-09-08 | 2017-11-24 | 广州威佰昆生物科技有限公司 | Breeding method of aedes albopictus oviposition female mosquito |
| CN107372370A (en) * | 2017-09-08 | 2017-11-24 | 广州威佰昆生物科技有限公司 | Hatching solution for hatching aedes albopictus eggs and hatching method of aedes albopictus eggs |
| CN107372371B (en) * | 2017-09-08 | 2020-05-12 | 广州威佰昆生物科技有限公司 | Breeding method of aedes albopictus oviposition female mosquito |
| CN108094768A (en) * | 2017-12-07 | 2018-06-01 | 吉林农业大学 | It cultivates wriggler stalk fermentation matrix and its cultivates wriggler method |
| CN108094768B (en) * | 2017-12-07 | 2021-05-28 | 吉林农业大学 | Wiggler straw fermentation substrate and wiggler cultivation method thereof |
| CN113712137A (en) * | 2021-08-11 | 2021-11-30 | 广州威佰昆生物科技有限公司 | Artificial feed for culex larvae and artificial feeding method |
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| Publication number | Publication date |
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| CN107018956B (en) | 2019-06-21 |
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