CN107361028A - A kind of dilution method for breeding of aphidius gifuensis - Google Patents
A kind of dilution method for breeding of aphidius gifuensis Download PDFInfo
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- CN107361028A CN107361028A CN201710823098.4A CN201710823098A CN107361028A CN 107361028 A CN107361028 A CN 107361028A CN 201710823098 A CN201710823098 A CN 201710823098A CN 107361028 A CN107361028 A CN 107361028A
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- aphid
- honeybee
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- tobacco seedlings
- dilution
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- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 39
- 238000009395 breeding Methods 0.000 title claims abstract description 22
- 230000001488 breeding effect Effects 0.000 title claims abstract description 21
- 238000003113 dilution method Methods 0.000 title claims abstract description 15
- 241001124076 Aphididae Species 0.000 claims abstract description 82
- 241000208125 Nicotiana Species 0.000 claims abstract description 78
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims abstract description 78
- 241000256844 Apis mellifera Species 0.000 claims abstract description 58
- 238000010790 dilution Methods 0.000 claims abstract description 31
- 239000012895 dilution Substances 0.000 claims abstract description 31
- 241000196324 Embryophyta Species 0.000 claims abstract description 29
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 21
- 238000009331 sowing Methods 0.000 claims abstract description 8
- 238000007865 diluting Methods 0.000 claims abstract description 7
- 241000282326 Felis catus Species 0.000 claims abstract description 6
- 238000007726 management method Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 16
- 239000003337 fertilizer Substances 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 7
- 239000005794 Hymexazol Substances 0.000 claims description 6
- KGVPNLBXJKTABS-UHFFFAOYSA-N hymexazol Chemical compound CC1=CC(O)=NO1 KGVPNLBXJKTABS-UHFFFAOYSA-N 0.000 claims description 6
- 210000003608 fece Anatomy 0.000 claims description 4
- 239000010871 livestock manure Substances 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- 201000010099 disease Diseases 0.000 claims description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 3
- 238000009792 diffusion process Methods 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 241000238631 Hexapoda Species 0.000 abstract description 2
- 235000019994 cava Nutrition 0.000 description 11
- 230000000384 rearing effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 241000422846 Sequoiadendron giganteum Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000032669 eclosion Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000024241 parasitism Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of dilution method for breeding of aphidius gifuensis, belong to cultured insect technical field.The dilution method for breeding includes cultivating host plant and aphidius gifuensis raising, it is characterised in that：Described cultivation host plant and aphidius gifuensis raising are made up of the step of following orders：（1）Host's tobacco seedlings are cultivated, cultivate host's tobacco seedlings using floating seedlings stamper, every square metre is cultivated 1500 plants, and the thickness of sowing of floating seedlings stamper is 300 400 plants/disk；（2）Tobacco seedlings dilute, and when tobacco seedlings length to the cat ear phase, the strong sprout falsity in tobacco seedlings are diluted into floating seedlings sub-disk, dilution density is 160-320 plants every square metre；(3) management of tobacco seedlings after diluting；(4) aphid is connect；(5) honeybee is connect；(6) feeding management after honeybee is connect.The present invention has the beneficial effect for improving the effective rate of utilization of cigarette strain, improving raising quality, reduce feeding cost, reducing raising risk.
Description
Technical field
The invention belongs to cultured insect technical field, and in particular to a kind of dilution method for breeding of aphidius gifuensis.
Background technology
At present, aphidius gifuensis biological control cigarette aphid is in large-scale promotion application stage, the popularization and application master of the technology
To include Scale dependency and dissipate two parts with field, aphidius gifuensis is raised using the numerous honeybee mode of seedling by floating seedlings.Passing
Unite in floating seedlings raising aphidius gifuensis method, to ensure tobacco seedlings quality, ensure the living space of cigarette aphid and aphidius gifuensis, tobacco seedlings
Cigarette kind is not typically broadcast in 100-150 strains/disk, so about 400-600 Zhu/㎡, part without full seeding, thickness of sowing during cultivation
Hole matrix be wasted.When being sowed using the big porose disc such as 160 caves, density is suitable, but matrix usage amount can be multiplied,
The cost of raising aphidius gifuensis increases therewith.
In addition, either entered using the apertures such as 504 caves drift disk or 160 caves etc. macropore drift disk, tobacco seedlings after the cat ear phase
Fast growing, competing with one another between tobacco seedlings, mutually block, some weak seedlings can not grow, and cause waste, meanwhile, it have compressed cigarette
Aphid and the living space of aphidius gifuensis, the breeding of cigarette aphid is caused to decline, aphidius gifuensis parasitism efficiency declines, and the raising later stage, which easily causes, posts
Raw aphid comes off and has a strong impact on raise benefit.
Correlative study and the report of aphidius gifuensis dilution method for breeding are yet there are no in the prior art.
The content of the invention
For deficiencies of the prior art, it is an object of the invention to provide a kind of dilution raising side of aphidius gifuensis
Method, its technical problems to be solved improve the effective rate of utilization of cigarette strain, improve raising quality, reduce feeding cost, reduce raising
Risk.
To realize upper art goal of the invention, the present invention uses following technical proposals：A kind of dilution method for breeding of aphidius gifuensis,
Including cultivating host plant and aphidius gifuensis raising, it is characterised in that：Described cultivation host plant and aphidius gifuensis raising are
It is made up of the step of following orders：
（1）Cultivate host's tobacco seedlings：Host's tobacco seedlings are cultivated using floating seedlings stamper, every square metre is cultivated 1500 plants, floating seedlings
The thickness of sowing of stamper is 300-400 strains/disk；
（2）Tobacco seedlings dilute：When tobacco seedlings length to the cat ear phase, the strong sprout falsity in tobacco seedlings is diluted to floating seedlings sub-disk, diluted
Density is 160-320 plants every square metre；
(3) management of tobacco seedlings after diluting：A shelters from heat or light：The management of sheltering from heat or light for a few days ago carrying out whole day after dilution, the root since the 3rd day
Shelter from heat or light the time according to weather condition adjustment, avoid tobacco seedlings from being shone and wilt；B water and fertilizer managements:During dilution apply Special compound fertilizer for tobacco or
Tobacco proprietary formula seed manure, amount of application are 2kg/ ton water, are in advance uniformly applied fertilizer to after being completely dissolved again with bubble during use
In the water for entering nursery pond;C disease controls：Transplanting spirit or hymexazol are applied in the water of nursery pond, transplants the amount of application of spirit
For 30ml/ ton water, hymexazol amount of application is 10ml/ ton water；
(4) aphid is connect：2-3 days after dilution, aphid is connect in tobacco seedlings also after seedling, aphid is connect and is connect using honeybee aphid tap or honeybee aphid are same；
(5) honeybee is connect:Honeybee is connect to connect using honeybee aphid tap or honeybee aphid are same；
(6) feeding management after honeybee is connect：To see that based on light, noon temperature is higher, and the situation that tobacco seedlings have wilting is hidden during numerous aphid
It is cloudy；During numerous honeybee based on sheltering from heat or light, except the cloudy, rainy day does not take management of sheltering from heat or light, fine day daily 10:00-18:Shade net is covered when 00.
The present invention is in step（2）After the tobacco seedlings dilution, remaining weak seedling can continue to raise cigarette aphid on floating seedlings stamper
Cocoon honeybee.
The present invention is in step（4）Connecing after the dilution described in aphid honeybee aphid the is adopted within 2-3 days after tobacco seedlings dilution with the method connect
With connection is put, carry out connecing aphid according to 7-10 heads/plant, collected after the transfer of cigarette aphid and connect aphid material.
The present invention is in step（4）Connecing the method that honeybee aphid taps after the dilution described in aphid the is adopted within 2-3 days after tobacco seedlings dilution
With natural diffusion method or connection is put, carries out connecing aphid according to 2-5 heads/plant.
The present invention is in step（5）It is when aphid amount is to 50 heads/plant, according to 1 that honeybee, which is connect, using the method for honeybee aphid tap:50 honeybee
Aphid ratio connects honeybee.
Compared with prior art, beneficial effects of the present invention have：
1st, the method can significantly improve the effective rate of utilization of cigarette strain.Using the method heeled in, by unit area tobacco seedlings from high density
It is diluted to low-density.Such as heeled in from 504 caves by seedling to 160 caves, a disk can heel in 4-6 disks, and raising carrier adds 4-6
Times, the increase of rear tobacco seedlings living space is heeled in, tobacco seedlings growing way is preferable, and each strain can act as dissipating material, the cigarette strain after diluting,
Growing way is more preferable, individual plant raising aphidius gifuensis quantity increase.Tobacco seedlings weaker on stamper are stayed in when heeling in can continue to raise cigarette aphid cocoon
Honeybee, the utilization rate of tobacco seedlings further improve, and following table is that the feeding efficiency of conventional high density raising and present invention dilution raising contrasts.
Table 1：Conventional high density is raised to be contrasted with the feeding efficiency of dilution raising
| Project contrasts | High-density rearing's method | Dilute method for breeding | Remarks |
| Thickness of sowing | 100-150 strains/disk | 300-400 strains/disk | |
| Rearing tray number | 1 disk | 4 disks | Stamper is added depending on thickness of sowing, during statistics. |
| Effective tobacco seedlings number | 60-80 strains | 300-400 strains | Effective strain number about 60-80 strains in conventinal breeding, remaining 40 plants or so tobacco seedlings are too weak, and it is few that aphidius gifuensis quantity is raised in tobacco seedlings, it is impossible to As material is dissipated, the cigarette strain after dilution can be carried out dissipating. |
| Unit area feeding quantity | 24600 Tou/㎡ | 24480 Tou/㎡ | Every square metre of dilution raising higher density raising difference is little. |
2nd, the present invention can significantly reduce feeding cost.Exemplified by sowing 324 plants/disk using 504 caves drift disk, dilution raising is first will
Tobacco seedlings educate the floating seedlings stamper to 504 caves, are heeled in again after tobacco seedlings length to cat phase to the floating seedlings sub-disk in 160 caves, whole
Matrix and the dosage of seed are saved during individual, can heel in 4 disk tobacco seedlings by 1 disk mother seedling calculates, plus stamper, i.e., using dilution
Method for breeding, 5 disk aphidius gifuensis can be raised by cultivating 1 disk tobacco seedlings, and table 2, which calculates, has raised 1000 disk aphidius gifuensis conventinal breeding methods
With dilution method for breeding needed for feeding cost.
Table 2：Conventinal breeding aphidius gifuensis contrasts with dilution raising aphidius gifuensis feeding cost
| Project | Conventinal breeding | Dilution raising |
| Rearing tray number | 1000 disks | 1000 disks |
| Raise goods and materials | 7676.77 | 4959.30 |
| Cost of labor | 2900.00 | 2100.00 |
| It is total | 10576.80 | 7059.30 |
According to table 2 as can be seen that the disk aphidius gifuensis of conventinal breeding 1000 needs 10576.80 yuan, dilution 1000 disk cigarette aphid cocoons of raising
Honeybee needs 7059.30 yuan, and more conventional raising is raised in dilution can save 3517.50 yuan.
3rd, the method can significantly improve raising quality.Tobacco seedlings are heeled in 160 cave sub-disks from 504 cave stampers, and often disk is only heeled in
40 plants, preferable living space is created for tobacco seedlings, tobacco seedlings growing way is numerous significantly better than the tobacco seedlings that the numerous honeybee of traditional floating seedlings is cultivated
The increase of honeybee leaf area.Vertical space increase after heeling in, solid space is more open, is more beneficial for the life of cigarette aphid and aphidius gifuensis
Long development, the stiff aphid raised out is bigger, fuller, and the aphidius gifuensis head of sprouting wings is bigger, and activity is stronger.
4th, the present invention can significantly reduce raising risk.When heeling in 160 caves drift disk, it is empty that tobacco seedlings obtain preferably existence
Between, tobacco seedlings growing way is substantially better than the tobacco seedlings that the numerous honeybee of conventional floating seedlings is cultivated, the enhancing of tobacco seedlings resistance, is not easy susceptible, there is foot
Enough nutrition supply cigarette aphids, the dead probability of parasitic aphid reduce, and raising risk decreases.
Brief description of the drawings
Fig. 1 is to heel in tobacco seedlings distribution schematic diagram in 160 cave floating seedlings sub-disks,
In figure, to heel in tobacco seedlings hole, white hole is heeled in without tobacco seedlings in the hole of black.
Embodiment
Below by embodiment, the present invention is further illustrated, but is not limited to embodiment.
Embodiment 1
A kind of dilution method for breeding of aphidius gifuensis, including host's tobacco seedlings and aphidius gifuensis raising are cultivated, before cultivating host's tobacco seedlings
First to choose raising place and build up Rearing facility, aphidius gifuensis raising includes connecing aphid, connect honeybee and connecing feeding management after honeybee, has
Body step is as follows：
1. raising farm selects：（1）Physical features is flat, leeward to face south, around without tall and big trees, building sunshade；（2) water source is convenient
And it is pollution-free, while away from pollution sources, such as vegetable plot, lavatory, plant place；（3）Have a good transport service, be easy to management and cigarette aphid cocoon
Honeybee field dissipates.
2. Rearing facility requires：Frame is made by the composite of steel pipe, thin-walled lightweight pipe or high intensity；Canopy film requirement is saturating
Light rate is high, extremely efficient in preserving heat, tension stress, and the ability of anti-agriculture chemical is strong.It is general to select the polyvinyl chloride plastic that thickness is 0.10 millimeter
Expect film or without the long-lived film of drop.Canopy both sides set vent window, and vent window is isolated with 60 mesh fly nets.
3. cultivate host's tobacco seedlings：
（1）Host's tobacco seedlings select, and select disease resistance strong, the non-aphid resistance kind of suitable cigarette aphid feeding, such as MSK326, Yunyan202
Deng.
（2）Sowing, using 504 caves, 360 caves or 325 cave floating seedling trays as stamper, sowed according to 1500 Zhu/㎡, list
Disk density adjusts according to dish-type.
（3）Management after planting：After planting based on heat and moisture preserving, light is seen sooner or later when tobacco seedlings start to grow young leaves, daily
9:00-18:00 shelters from heat or light, and starting whole day when tobacco seedlings length to 31 heart of leaf sees light.
（4）Tobacco seedlings dilute：When tobacco seedlings length to the cat ear phase, tobacco seedlings are diluted in 160 cave floating seedlings sub-disks, density
160-320 Zhu/㎡, such as strong sprout is heeled in into 160 cave floating seedlings sub-disks by 40 plants/disk.
Tobacco seedlings distribution schematic diagram, which is heeled in, in 160 cave floating seedlings sub-disks sees Fig. 1.
(5) tobacco seedlings management after diluting:The management of sheltering from heat or light for a few days ago carrying out whole day after dilution, according to weather feelings after two days
Condition adjustment is sheltered from heat or light the time, daily 10 during fine day:00-16:00 carries out management of sheltering from heat or light, and cloudy day, rainy day are reduced in good time shelters from heat or light the time,
Avoid tobacco seedlings from being shone to wilt.
(6) water and fertilizer management:Special compound fertilizer for tobacco or tobacco proprietary formula seed manure are applied during dilution, is 2kg/ using standard
Ton water, fertilizer is applied in pond with bubble is uniform again to after being completely dissolved in advance.
(7) disease control:The application transplanting spirit of pool water within or hymexazol, the use standard for transplanting spirit are after dilution
30ml/ ton water, hymexazol are 10ml/ ton water using standard.
4th, aphid is connect：Connect aphid after the dilution, connect aphid mode be divided into honeybee aphid with connect and honeybee aphid tap.
Same connect of honeybee aphid connects aphid using honeybee aphid seedling, and method is to carry out within 2-3 days connecing aphid after diluting, and connects aphid amount as 7-10 heads/plant, adopts
Aphid is connect with connection is put, receipts go to connect aphid material after treating the transfer of cigarette aphid.
Honeybee aphid tap connects aphid from pure aphid, and method is to carry out within 2-3 days connecing aphid after diluting, and using natural diffuseness or puts connection,
Aphid is connect by 2-5 heads/plant.
5th, honeybee is connect：Honeybee is connect to connect using honeybee aphid tap or honeybee aphid are same.
The method of honeybee aphid tap is when aphid amount is to 50 heads/plant, according to 1:50 honeybee aphid ratio connects honeybee.
Honeybee aphid is same to be connect：It is when kind of bee eclosion, according to the situation and Aphed population and quality of kind of honeybee activity, judges whether to need
Mend honeybee；
5th, managed after connecing honeybee：
（1）Shelter from heat or light management：To see that based on light, noon temperature is higher, and the situation that tobacco seedlings have wilting can suitably shelter from heat or light during numerous aphid；It is numerous
During honeybee based on sheltering from heat or light, except the cloudy, rainy day does not take management of sheltering from heat or light, under bright day gas, daily 10:00-18:Covering is sheltered from heat or light when 00
Net, to reduce temperature and reduce illumination.
（2）Water and fertilizer management：Need to monitor EC values in real time after connecing honeybee（Soluble-salt concentration）, according to the appropriate supplementary fertilizer of EC values, avoid
The few fertilizer of water shortage, ensure that liquid manure is sufficient during raising.
（3）Leaf-cutting management：According to the appropriate leaf-cutting of tobacco seedlings size, but leaf-cutting number is usually no more than 3 times, during having honeybee activity
General not leaf-cutting.
（4）Medicine management：Collect connect aphid material and leaf-cutting after squirt 1000 times of carbendazim, prevent fungus disease.
Host's tobacco seedlings of the present invention can change other host plants, such as radish, rape into.The technology be also applied for except
Raise the aphid beyond cigarette aphid and aphidius gifuensis and aphid parasite.
Claims (5)
1. a kind of dilution method for breeding of aphidius gifuensis, including cultivate host plant and aphidius gifuensis raising, it is characterised in that：Institute
The cultivation host plant stated and aphidius gifuensis raising are made up of the step of following orders：
（1）Cultivate host's tobacco seedlings：Host's tobacco seedlings are cultivated using floating seedlings stamper, every square metre is cultivated 1500 plants, floating seedlings
The thickness of sowing of stamper is 300-400 strains/disk；
（2）Tobacco seedlings dilute：When tobacco seedlings length to the cat ear phase, the strong sprout falsity in tobacco seedlings is diluted to floating seedlings sub-disk, diluted
Density is 160-320 plants every square metre；
(3) management of tobacco seedlings after diluting：A shelters from heat or light：The management of sheltering from heat or light for a few days ago carrying out whole day after dilution, the root since the 3rd day
Shelter from heat or light the time according to weather condition adjustment, avoid tobacco seedlings from being shone and wilt；B water and fertilizer managements:During dilution apply Special compound fertilizer for tobacco or
Tobacco proprietary formula seed manure, amount of application are 2kg/ ton water, are in advance uniformly applied fertilizer to after being completely dissolved again with bubble during use
In the water for entering nursery pond;C disease controls：Transplanting spirit or hymexazol are applied in the water of nursery pond, transplants the amount of application of spirit
For 30ml/ ton water, hymexazol amount of application is 10ml/ ton water；
(4) aphid is connect：2-3 days after dilution, aphid is connect in tobacco seedlings also after seedling, aphid is connect and is connect using honeybee aphid tap or honeybee aphid are same；
(5) honeybee is connect:Honeybee is connect to connect using honeybee aphid tap or honeybee aphid are same；
(6) feeding management after honeybee is connect：To see that based on light, noon temperature is higher, and the situation that tobacco seedlings have wilting is hidden during numerous aphid
It is cloudy；During numerous honeybee based on sheltering from heat or light, except the cloudy, rainy day does not take management of sheltering from heat or light, fine day daily 10:00-18:Shade net is covered when 00.
A kind of 2. dilution method for breeding of aphidius gifuensis according to claim 1, it is characterised in that：In step（2）It is described
After tobacco seedlings dilution, remaining weak seedling can continue to raise aphidius gifuensis on floating seedlings stamper.
A kind of 3. dilution method for breeding of aphidius gifuensis according to claim 1, it is characterised in that：In step（4）Connect aphid
Honeybee aphid is to use within the 2-3 days after tobacco seedlings dilution to put connection with the method connect after described dilution, is connect according to 7-10 heads/plant
Aphid, collected after the transfer of cigarette aphid and connect aphid material.
A kind of 4. dilution method for breeding of aphidius gifuensis according to claim 1, it is characterised in that：In step（4）Connect aphid
The method that honeybee aphid taps after described dilution is to use natural diffusion method or put connection for the 2-3 days after tobacco seedlings dilution, according to 2-
5 heads/plant carry out connecing aphid.
A kind of 5. dilution method for breeding of aphidius gifuensis according to claim 1, it is characterised in that：In step（5）Connect honeybee
The method tapped using honeybee aphid is when aphid amount is to 50 heads/plant, according to 1:50 honeybee aphid ratio connects honeybee.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN204776833U (en) * | 2015-07-13 | 2015-11-18 | 陈治锋 | Specialized transportation tray of tobacco seedling |
| CN205143033U (en) * | 2015-11-10 | 2016-04-13 | 四川省烟草公司攀枝花市公司（攀枝花市烟草公司） | Tobacco floats special dish of growing seedlings |
| CN104126551B (en) * | 2014-08-15 | 2016-06-29 | 云南省烟草公司红河州公司 | A kind of method of floating tobacco seedlings breeding aphidius gifuensis |
-
2017
- 2017-09-13 CN CN201710823098.4A patent/CN107361028A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| CN104126551B (en) * | 2014-08-15 | 2016-06-29 | 云南省烟草公司红河州公司 | A kind of method of floating tobacco seedlings breeding aphidius gifuensis |
| CN204776833U (en) * | 2015-07-13 | 2015-11-18 | 陈治锋 | Specialized transportation tray of tobacco seedling |
| CN205143033U (en) * | 2015-11-10 | 2016-04-13 | 四川省烟草公司攀枝花市公司（攀枝花市烟草公司） | Tobacco floats special dish of growing seedlings |
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